CmaCh01G020460 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G020460
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionsplicing factor U2af small subunit B-like
LocationCma_Chr01: 13006431 .. 13007261 (-)
RNA-Seq ExpressionCmaCh01G020460
SyntenyCmaCh01G020460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAACACTTGGCCTCAATATTTGGCACCGAGAAGGACCGTGTCAACTGCCCTTTCTATTTCAAGATCGGCGCCTGCCGTCATGGAGATCGTTGCTCTCGCCTCCACAACCGCCCTACTATTTCCCCCACTCTTCTCTTATCGAACATGTACCAGCGCCCCGACATGATCACCCCTGGTGTCGATGCTCAGGGTCAGCCCATAGATCCCCGCAAGATTCAGGAGCACTTCGAGGATTTCTACGAAGACATTTACGAGGAACTCGGAAAGTTCGGTGAGATCGAGAGCCTCAACATCTGTGATAACCTCGCCGACCACATGATCGGCAATGTTTACGTTCAGTTTAGGGAGGAGGACCAGGCTGCTGCCGCACTTCAAGCGTTGCAAGGGCGATTCTACTCTGGTCGACCCATAATCGCAGATTTTTCTCCGGTGACAGATTTCCGCGAGGCCACGTGCCGCCAGTACGAGGAGAATAATTGTAATCGAGGTGGATATTGCAATTTTATGCATGTTAAGATGATTGGGAAGGACTTGAGGAGGAAGCTGTTCGGTAGTAGGTTCCGGGGTTATAGGCGGAGCCGTAGCAGGAGTAGAAGTTTGAGTCCGCGGAACCGGAAGGACTACGATAGGCGTGAGAGGGATTATAGAGATCGTGACCATCGTGGAAACGGACGGAGCAAGGAGCGTCCAGATAGGTATGACAGAGATGGGGGAAGGAGAAGGCATGGAAGCCCCAGGCGAAGCAGAAGTCCTGTCAGAGAAGGAAGCGAAGAGCGCCGAGCCCGTATTGAACAGTGGAACAGAGAGAGGGAGGAGAAGCAGTGA

mRNA sequence

ATGGCTGAACACTTGGCCTCAATATTTGGCACCGAGAAGGACCGTGTCAACTGCCCTTTCTATTTCAAGATCGGCGCCTGCCGTCATGGAGATCGTTGCTCTCGCCTCCACAACCGCCCTACTATTTCCCCCACTCTTCTCTTATCGAACATGTACCAGCGCCCCGACATGATCACCCCTGGTGTCGATGCTCAGGGTCAGCCCATAGATCCCCGCAAGATTCAGGAGCACTTCGAGGATTTCTACGAAGACATTTACGAGGAACTCGGAAAGTTCGGTGAGATCGAGAGCCTCAACATCTGTGATAACCTCGCCGACCACATGATCGGCAATGTTTACGTTCAGTTTAGGGAGGAGGACCAGGCTGCTGCCGCACTTCAAGCGTTGCAAGGGCGATTCTACTCTGGTCGACCCATAATCGCAGATTTTTCTCCGGTGACAGATTTCCGCGAGGCCACGTGCCGCCAGTACGAGGAGAATAATTGTAATCGAGGTGGATATTGCAATTTTATGCATGTTAAGATGATTGGGAAGGACTTGAGGAGGAAGCTGTTCGGTAGTAGGTTCCGGGGTTATAGGCGGAGCCGTAGCAGGAGTAGAAGTTTGAGTCCGCGGAACCGGAAGGACTACGATAGGCGTGAGAGGGATTATAGAGATCGTGACCATCGTGGAAACGGACGGAGCAAGGAGCGTCCAGATAGGTATGACAGAGATGGGGGAAGGAGAAGGCATGGAAGCCCCAGGCGAAGCAGAAGTCCTGTCAGAGAAGGAAGCGAAGAGCGCCGAGCCCGTATTGAACAGTGGAACAGAGAGAGGGAGGAGAAGCAGTGA

Coding sequence (CDS)

ATGGCTGAACACTTGGCCTCAATATTTGGCACCGAGAAGGACCGTGTCAACTGCCCTTTCTATTTCAAGATCGGCGCCTGCCGTCATGGAGATCGTTGCTCTCGCCTCCACAACCGCCCTACTATTTCCCCCACTCTTCTCTTATCGAACATGTACCAGCGCCCCGACATGATCACCCCTGGTGTCGATGCTCAGGGTCAGCCCATAGATCCCCGCAAGATTCAGGAGCACTTCGAGGATTTCTACGAAGACATTTACGAGGAACTCGGAAAGTTCGGTGAGATCGAGAGCCTCAACATCTGTGATAACCTCGCCGACCACATGATCGGCAATGTTTACGTTCAGTTTAGGGAGGAGGACCAGGCTGCTGCCGCACTTCAAGCGTTGCAAGGGCGATTCTACTCTGGTCGACCCATAATCGCAGATTTTTCTCCGGTGACAGATTTCCGCGAGGCCACGTGCCGCCAGTACGAGGAGAATAATTGTAATCGAGGTGGATATTGCAATTTTATGCATGTTAAGATGATTGGGAAGGACTTGAGGAGGAAGCTGTTCGGTAGTAGGTTCCGGGGTTATAGGCGGAGCCGTAGCAGGAGTAGAAGTTTGAGTCCGCGGAACCGGAAGGACTACGATAGGCGTGAGAGGGATTATAGAGATCGTGACCATCGTGGAAACGGACGGAGCAAGGAGCGTCCAGATAGGTATGACAGAGATGGGGGAAGGAGAAGGCATGGAAGCCCCAGGCGAAGCAGAAGTCCTGTCAGAGAAGGAAGCGAAGAGCGCCGAGCCCGTATTGAACAGTGGAACAGAGAGAGGGAGGAGAAGCAGTGA

Protein sequence

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDLRRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGGRRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ
Homology
BLAST of CmaCh01G020460 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 3.8e-127
Identity = 237/281 (84.34%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVD QGQP+DP KIQ+HFEDFYEDI+EEL KFGE+ESLN+CDNLADHMIGNVYV F+EED
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
            AAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEEN+CNRGGYCNFMHVK I ++L
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRKLFGSRFRGYRR-SRSRSRSLSPRNRKDYDR-RER-DYRDRDHRGNG-RSKERPDRYD 240
           RRKLFG   R YRR SRSRSRS+SPR ++++ R RER D RDRD  GNG RS +R +R+D
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 RD-GGRRRHGSPRRSRSP--VREGSEERRARIEQWNREREE 275
           RD GGRRRHGSP+RSRSP  VREGSEERRARIEQWNRER+E
Sbjct: 241 RDGGGRRRHGSPKRSRSPRNVREGSEERRARIEQWNRERDE 281

BLAST of CmaCh01G020460 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 5.5e-126
Identity = 237/292 (81.16%), Postives = 257/292 (88.01%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQP+DPRKIQEHFEDF+ED++EELGKFGEIESLNICDNLADHMIGNVYVQF+EED
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVK++ ++L
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RRKLFGSRFRGYR---RSRSRSRSLSPRNRKDYDRRERDYRDRDHR---------GNG-R 240
           RRKLFG   R YR   RSRSRSRS+SPRN++D DRR+  +R+  HR         G+G R
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

Query: 241 SKERPDRYDRDGGR-RRHGSPRRSRSP--VREGSEERRARIEQWNREREEKQ 277
           S ER +R +RDG R RR  SP+R  SP   REGSEERRARIEQWNREREEK+
Sbjct: 241 SSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNREREEKE 292

BLAST of CmaCh01G020460 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 1.8e-116
Identity = 227/306 (74.18%), Postives = 248/306 (81.05%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPT+SPT++L+NMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDP K+QEHFEDFYEDIYEEL KFGE+E+LN+CDNLADHMIGNVYVQFREE+
Sbjct: 61  GVDAQGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEE 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QA AA  ALQGRFYSGRPII ++SPVTDFREATCRQ+EEN+CNRGGYCNFMHVK IG++L
Sbjct: 121 QAVAAHNALQGRFYSGRPIIVEYSPVTDFREATCRQFEENSCNRGGYCNFMHVKQIGREL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNR-----KDYDRRERD------------------- 240
           RRKL+G R    RRS  RSRS SPR+R     +D  RRERD                   
Sbjct: 181 RRKLYGGR---SRRSHGRSRSPSPRHRRGNRDRDDFRRERDGYRGGGDGYRGGGGGGGGD 240

Query: 241 -YRDRD-HRGNGRSKER--PDRYDR--DGGRRRHGS-PRRSRSPVREGSEERRARIEQWN 276
            YR  D +RG G    R    RYDR  DGGRRRHGS PRR+RSPVRE SEERRA+IEQWN
Sbjct: 241 GYRGGDSYRGGGGGGRRGGGSRYDRYDDGGRRRHGSPPRRARSPVRESSEERRAKIEQWN 300

BLAST of CmaCh01G020460 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 8.5e-111
Identity = 216/293 (73.72%), Postives = 236/293 (80.55%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHN+P++SPTLLLSNMY RPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           G+DAQG PIDP KIQ  FEDFYEDI+EEL K+GEIESL++CDN ADHMIGNVYVQFREED
Sbjct: 61  GIDAQGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAA ALQAL GR+YSGRPII +FSPV+DFREATCRQYEEN+CNRGGYCNFMHVK IG+DL
Sbjct: 121 QAARALQALTGRYYSGRPIIVEFSPVSDFREATCRQYEENSCNRGGYCNFMHVKEIGRDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSP---RNRKDYDRRERDYRDRDH-------------RG 240
           R++LFG   R  RRS S  RS SP     R+DYDRR    R RDH              G
Sbjct: 181 RKRLFGHLHRS-RRSHSHGRSRSPSPYHYRRDYDRRSSS-RSRDHDDYYRGGSHDYYRGG 240

Query: 241 NGRSKER-PDRYDRDGGRRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           + RS ER    YD DG RRRH S  R+RSPVR+GSEERRA+IEQWNRERE  Q
Sbjct: 241 SRRSSERHRSSYDSDGSRRRHRS--RTRSPVRDGSEERRAQIEQWNREREAAQ 289

BLAST of CmaCh01G020460 vs. ExPASy Swiss-Prot
Match: A1A4K8 (Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.2e-65
Identity = 133/258 (51.55%), Postives = 172/258 (66.67%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAE+LASIFGTEKD+VNC FYFKIGACRHGDRCSRLHN+PT S T+ L N+Y+ P   + 
Sbjct: 1   MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQ 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEEL-GKFGEIESLNICDNLADHMIGNVYVQFREE 120
             D     +   ++QEH+++F+E+++ E+  K+GE+E +N+CDNL DH++GNVYV+FR E
Sbjct: 61  SADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRRE 120

Query: 121 DQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKD 180
           + A  A+  L  R+++G+PI A+ SPVTDFREA CRQYE   C RGG+CNFMH+K I ++
Sbjct: 121 EDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRE 180

Query: 181 LRRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDG 240
           LRR+L+G R R   RSRSRSR          +RR R  RDR   G G            G
Sbjct: 181 LRRELYGRR-RKKHRSRSRSR----------ERRSRS-RDRGRGGGG-----------GG 235

Query: 241 GRRRHGSPRRSRSPVREG 258
           G  R    RRSR   R G
Sbjct: 241 GGGRERDRRRSRDRERSG 235

BLAST of CmaCh01G020460 vs. ExPASy TrEMBL
Match: A0A6J1IV82 (splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111480904 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 4.9e-154
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG
Sbjct: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 276

BLAST of CmaCh01G020460 vs. ExPASy TrEMBL
Match: A0A6J1FSM7 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111446512 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 4.9e-154
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG
Sbjct: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 276

BLAST of CmaCh01G020460 vs. ExPASy TrEMBL
Match: A0A6J1CN17 (splicing factor U2af small subunit B-like OS=Momordica charantia OX=3673 GN=LOC111012985 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.4e-148
Identity = 269/275 (97.82%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFG R+RGYRRSRSRSRSLSPRNRKDYDRRERD+RDRD RGNGRSKER DRYDRDGG
Sbjct: 181 RRKLFG-RYRGYRRSRSRSRSLSPRNRKDYDRRERDFRDRDFRGNGRSKERYDRYDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEK 276
           RRRHGSPRRSRSPVREGSEERRARIEQWNREREEK
Sbjct: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEK 274

BLAST of CmaCh01G020460 vs. ExPASy TrEMBL
Match: A0A6J1F6R3 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111442843 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.1e-144
Identity = 266/278 (95.68%), Postives = 269/278 (96.76%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIE LN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIECLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYR--RSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRD 240
           RRKLFG R RGYR  RSRSRSRSLSPRNRK+ DRRERDYRDR+ RGNGRSKER DRYDRD
Sbjct: 181 RRKLFG-RHRGYRRSRSRSRSRSLSPRNRKELDRRERDYRDRESRGNGRSKERHDRYDRD 240

Query: 241 GGRRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           GGRRRHGSPRRSRSPVREGS ERRARIEQWNREREEKQ
Sbjct: 241 GGRRRHGSPRRSRSPVREGSVERRARIEQWNREREEKQ 277

BLAST of CmaCh01G020460 vs. ExPASy TrEMBL
Match: A0A5A7VFL1 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00280 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 7.4e-142
Identity = 264/280 (94.29%), Postives = 271/280 (96.79%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFG R+RGYR SRSRSRSLSPRNRK+YDRRERDYR+R++RGNGRSKE   R+DRDGG
Sbjct: 181 RRKLFG-RYRGYRPSRSRSRSLSPRNRKEYDRRERDYREREYRGNGRSKE---RHDRDGG 240

Query: 241 RRRHGSPR--RSRSPV--REGSEERRARIEQWNREREEKQ 277
           RRRHGSPR  RSRSPV  REGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRSRSPVIAREGSEERRARIEQWNREREEKQ 276

BLAST of CmaCh01G020460 vs. NCBI nr
Match: XP_022941119.1 (splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022941120.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022941121.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022981902.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022981903.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022981905.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >KAG7037979.1 Splicing factor U2af small subunit A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 553.5 bits (1425), Expect = 1.0e-153
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG
Sbjct: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 276

BLAST of CmaCh01G020460 vs. NCBI nr
Match: XP_023525325.1 (splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023525326.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023525327.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 275/276 (99.64%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDR+DRDGG
Sbjct: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRFDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 276

BLAST of CmaCh01G020460 vs. NCBI nr
Match: XP_022142994.1 (splicing factor U2af small subunit B-like [Momordica charantia])

HSP 1 Score: 535.4 bits (1378), Expect = 2.9e-148
Identity = 269/275 (97.82%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFG R+RGYRRSRSRSRSLSPRNRKDYDRRERD+RDRD RGNGRSKER DRYDRDGG
Sbjct: 181 RRKLFG-RYRGYRRSRSRSRSLSPRNRKDYDRRERDFRDRDFRGNGRSKERYDRYDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEK 276
           RRRHGSPRRSRSPVREGSEERRARIEQWNREREEK
Sbjct: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEK 274

BLAST of CmaCh01G020460 vs. NCBI nr
Match: XP_023521110.1 (splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >KAG6591187.1 Splicing factor U2af small subunit A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024073.1 Splicing factor U2af small subunit A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526.6 bits (1355), Expect = 1.3e-145
Identity = 266/276 (96.38%), Postives = 269/276 (97.46%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIE LN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIECLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240
           RRKLFG R RGYRRSRSRSRSLSPRNRK+ DRRERDYRDR+ RGNGRSKER DRYDRDGG
Sbjct: 181 RRKLFG-RHRGYRRSRSRSRSLSPRNRKELDRRERDYRDRESRGNGRSKERHDRYDRDGG 240

Query: 241 RRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           RRRHGSPRRSRSPVREGS ERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRSPVREGSVERRARIEQWNREREEKQ 275

BLAST of CmaCh01G020460 vs. NCBI nr
Match: XP_022936161.1 (splicing factor U2af small subunit B-like [Cucurbita moschata])

HSP 1 Score: 521.5 bits (1342), Expect = 4.3e-144
Identity = 266/278 (95.68%), Postives = 269/278 (96.76%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIE LN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIECLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGSRFRGYR--RSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRD 240
           RRKLFG R RGYR  RSRSRSRSLSPRNRK+ DRRERDYRDR+ RGNGRSKER DRYDRD
Sbjct: 181 RRKLFG-RHRGYRRSRSRSRSRSLSPRNRKELDRRERDYRDRESRGNGRSKERHDRYDRD 240

Query: 241 GGRRRHGSPRRSRSPVREGSEERRARIEQWNREREEKQ 277
           GGRRRHGSPRRSRSPVREGS ERRARIEQWNREREEKQ
Sbjct: 241 GGRRRHGSPRRSRSPVREGSVERRARIEQWNREREEKQ 277

BLAST of CmaCh01G020460 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 455.7 bits (1171), Expect = 2.7e-128
Identity = 237/281 (84.34%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVD QGQP+DP KIQ+HFEDFYEDI+EEL KFGE+ESLN+CDNLADHMIGNVYV F+EED
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
            AAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEEN+CNRGGYCNFMHVK I ++L
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRKLFGSRFRGYRR-SRSRSRSLSPRNRKDYDR-RER-DYRDRDHRGNG-RSKERPDRYD 240
           RRKLFG   R YRR SRSRSRS+SPR ++++ R RER D RDRD  GNG RS +R +R+D
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 RD-GGRRRHGSPRRSRSP--VREGSEERRARIEQWNREREE 275
           RD GGRRRHGSP+RSRSP  VREGSEERRARIEQWNRER+E
Sbjct: 241 RDGGGRRRHGSPKRSRSPRNVREGSEERRARIEQWNRERDE 281

BLAST of CmaCh01G020460 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 455.7 bits (1171), Expect = 2.7e-128
Identity = 237/281 (84.34%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVD QGQP+DP KIQ+HFEDFYEDI+EEL KFGE+ESLN+CDNLADHMIGNVYV F+EED
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
            AAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEEN+CNRGGYCNFMHVK I ++L
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRKLFGSRFRGYRR-SRSRSRSLSPRNRKDYDR-RER-DYRDRDHRGNG-RSKERPDRYD 240
           RRKLFG   R YRR SRSRSRS+SPR ++++ R RER D RDRD  GNG RS +R +R+D
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 RD-GGRRRHGSPRRSRSP--VREGSEERRARIEQWNREREE 275
           RD GGRRRHGSP+RSRSP  VREGSEERRARIEQWNRER+E
Sbjct: 241 RDGGGRRRHGSPKRSRSPRNVREGSEERRARIEQWNRERDE 281

BLAST of CmaCh01G020460 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 451.8 bits (1161), Expect = 3.9e-127
Identity = 237/292 (81.16%), Postives = 257/292 (88.01%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120
           GVDAQGQP+DPRKIQEHFEDF+ED++EELGKFGEIESLNICDNLADHMIGNVYVQF+EED
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVK++ ++L
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RRKLFGSRFRGYR---RSRSRSRSLSPRNRKDYDRRERDYRDRDHR---------GNG-R 240
           RRKLFG   R YR   RSRSRSRS+SPRN++D DRR+  +R+  HR         G+G R
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

Query: 241 SKERPDRYDRDGGR-RRHGSPRRSRSP--VREGSEERRARIEQWNREREEKQ 277
           S ER +R +RDG R RR  SP+R  SP   REGSEERRARIEQWNREREEK+
Sbjct: 241 SSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNREREEKE 292

BLAST of CmaCh01G020460 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 343.2 bits (879), Expect = 1.9e-94
Identity = 187/242 (77.27%), Postives = 207/242 (85.54%), Query Frame = 0

Query: 51  MYQRPDMITPGVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIG 110
           MYQRPDMITPGVDAQGQP+DPRKIQEHFEDF+ED++EELGKFGEIESLNICDNLADHMIG
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQFREEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 170
           NVYVQF+EEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 120

Query: 171 MHVKMIGKDLRRKLFGSRFRGYR---RSRSRSRSLSPRNRKDYDRRERDYRDRDHR---- 230
           MHVK++ ++LRRKLFG   R YR   RSRSRSRS+SPRN++D DRR+  +R+  HR    
Sbjct: 121 MHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDR 180

Query: 231 -----GNG-RSKERPDRYDRDGGR-RRHGSPRRSRSP--VREGSEERRARIEQWNREREE 277
                G+G RS ER +R +RDG R RR  SP+R  SP   REGSEERRARIEQWNREREE
Sbjct: 181 EFYRHGSGKRSSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNREREE 240

BLAST of CmaCh01G020460 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 119.8 bits (299), Expect = 3.5e-27
Identity = 98/304 (32.24%), Postives = 150/304 (49.34%), Query Frame = 0

Query: 9   FGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDAQGQP 68
           FGTE+D+ +CPF+ K GACR G RCSR+H  P  S TLL+ NMY  P  IT   D +G  
Sbjct: 237 FGTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYNGPG-ITWEQD-EGLE 296

Query: 69  IDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREEDQAAAALQA 128
               + +  +E+FYED++ E  K+GE+ +  +C N + H+ GNVYV +R  + A  A Q+
Sbjct: 297 YTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAYQS 356

Query: 129 LQGRFYSGRPIIADFSPVTDFREATCRQYEEN---NCNRGGYCNFMH-VKMIGKDLR--- 188
           + GR+++G+ +  +F  ++ ++ A C +Y ++    C+RG  CNF+H  +  G D     
Sbjct: 357 INGRYFAGKQVNCEFVNISRWKVAICGEYMKSRLKTCSRGSACNFIHCFRNPGGDYEWAD 416

Query: 189 -------------RKLFG---SRFRGYRRSRSRSRS---LSPRNRKDYDRRER------- 248
                          LFG    +   +  S S + S   LS  + +   RR R       
Sbjct: 417 HDRPPPRFWIHKMTSLFGYSDEKHMEHESSGSLNDSISDLSTDSHRQPSRRSRSRDHDHA 476

Query: 249 ------DYRDRDHRGNGRSKERPDRYDRDGGRRRHG--SPRRSRSPVREGSEERRARIEQ 272
                  YR R + G+ +   R D+  R       G  SP R  S  RE  +ERR   + 
Sbjct: 477 NVGSTPSYRSRKYHGDTQDSTREDKLRRHAENCHDGDDSPSRDGSLEREMYKERRYAKDT 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMY53.8e-12784.34Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ... [more]
Q9S7095.5e-12681.16Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ... [more]
Q6AUG01.8e-11674.18Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9ZQW88.5e-11173.72Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
A1A4K89.2e-6551.55Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IV824.9e-154100.00splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FSM74.9e-154100.00splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1CN171.4e-14897.82splicing factor U2af small subunit B-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1F6R32.1e-14495.68splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5A7VFL17.4e-14294.29Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
XP_022941119.11.0e-153100.00splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022941120.1 s... [more]
XP_023525325.13.0e-15399.64splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_02352... [more]
XP_022142994.12.9e-14897.82splicing factor U2af small subunit B-like [Momordica charantia][more]
XP_023521110.11.3e-14596.38splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >KAG65911... [more]
XP_022936161.14.3e-14495.68splicing factor U2af small subunit B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G42820.22.7e-12884.34Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT5G42820.12.7e-12884.34Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT1G27650.13.9e-12781.16U2 snRNP auxiliary factor small subunit, putative [more]
AT1G27650.21.9e-9477.27U2 snRNP auxiliary factor small subunit, putative [more]
AT1G10320.13.5e-2732.24Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009145U2 auxiliary factor small subunitPRINTSPR01848U2AUXFACTORcoord: 165..177
score: 63.29
coord: 75..90
score: 54.26
coord: 103..125
score: 65.61
coord: 18..37
score: 85.68
coord: 37..57
score: 62.34
coord: 130..154
score: 63.64
IPR009145U2 auxiliary factor small subunitPANTHERPTHR12620U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNITcoord: 1..276
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 148..174
e-value: 0.0087
score: 25.3
coord: 13..39
e-value: 0.0068
score: 25.6
IPR000571Zinc finger, CCCH-typePFAMPF00642zf-CCCHcoord: 150..174
e-value: 1.4E-4
score: 21.7
coord: 14..38
e-value: 2.8E-7
score: 30.3
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 148..175
score: 11.413015
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 12..40
score: 13.065497
IPR003954RNA recognition motif domain, eukaryoteSMARTSM00361rrm2_1coord: 73..142
e-value: 1.7E-4
score: 30.9
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 43..145
e-value: 1.4E-30
score: 107.6
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 83..139
e-value: 1.0E-7
score: 31.6
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 44..146
score: 11.093571
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..243
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..276
NoneNo IPR availablePANTHERPTHR12620:SF35SPLICING FACTOR U2AF SMALL SUBUNIT Acoord: 1..276
NoneNo IPR availableCDDcd12539RRM_U2AF35Bcoord: 44..145
e-value: 6.88888E-61
score: 186.031
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 77..149

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G020460.1CmaCh01G020460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
cellular_component GO:0089701 U2AF complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030628 pre-mRNA 3'-splice site binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding