CmaCh01G019290 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G019290
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbifunctional nuclease 1-like
LocationCma_Chr01: 12535565 .. 12537055 (-)
RNA-Seq ExpressionCmaCh01G019290
SyntenyCmaCh01G019290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCAAAAGGACAATATCTGCTAGTAGTGGGTTTGAGCCGTTACGTGACTTTGTTTTTGTGTTCCCTGTCAATTCTTCATCTTTTGCAGTGGAAATGCCAAGCGTTCTGCTCATGGCTGCTGTGCGCAATGTTCCAATTGTATGTTTTCTTTGTCTTCTCTTGTGATCAGATATAATTCAAATTTCTCTTGAATTTGAATTATATTCATTGAAAGGGACTTTCTAACATATTATCTGTTTCCAGGCCAGACCAACCATGTATCAAGTGGTGAAAGAGATGATTGACAAGATGGGTTATGAAGTATGTTTTGCATTATTTCTCCATCTTTCCACCTTGTTTTTTGTAAGCTTAAATGGGATGTGGGCTCTTTCTAATGTTAATTACGAAGCAGGTCAAACTTGTAAGGGTTACCAAGAGAGTGCATGAGGCATATTTTGCCCAGTTATACCTCTCAAAGGCATGTTTTAGTTTCTCTCTTTGGATTCTTTACCCTTCTCTTAGGTAAGTTTAAGAGATTGAAATTTTATACGTTTCAGATTGGTTGTGAGAATGACTGCCTCAGCTTTGATCTTCGACCTTCAGATGCAATAAACATTGCAGTCCGATGCAAGGTAACTTAAATTTTTCGTGCTTAGTAGAGACTTCGGTCAAGTGCCTCTTTAAGTGGCCAAAGTATTGTTGAAATATGGGCTTTTGGAGAATTTGTTCATTTGCTATTGGTTATAGAATGTCTGGTAGTCATTTGTGTAAAGCTGTATTTGGATGTTTTTCATATTTTGGGCATGTGCTGTGTGTGCTGAATCATGTTACTTTTTGCAATTGTTGCAGGTCCCAATTCAAGTGAACAAGTACCTTGCATACAGCGACGGTATGCGAGTTGTTGAAACTGGGAAGTTGTCAACCCAGACCCCTGCTACGGACGGTTTACTATTCACCGAACTGGATAGGTAATAAGATATTTTTAGTGTATATTCGAGGAATAGGGGGAACGTTGATCTTTCAACAGCCATTTGAAACTTCTGCTTCATTTGATGAGAGGAAAGGGATAATTGATTTTTTTTTTGTTTTCAGGCCCAGTGGTCAGCCTTGTGTGGAAGCCAAAGAGTTTGATCTGGTGAGGAATATGCTGATTGCTGCTGTTGAAGAGCGCTACAGAGATGCTGGTATGGATTTCGTTGCTCAATTAACATTTTTCAATTCTGAATGTTCCAAATCAATTTTATTTCTCTGATTTAGAACAGAAACTTGCGTACTTAAGAATACCAAGAATAATTAATTGCTAATATGGGATGACCTTGTGGTGTTATGGATAGTTCTAAAAGTTCTTCCAAAGAATCCCTCTGTTTAGGACAAAAAATAAGCCCCATTTTCCCTTTTTGGTTATTCAATGTTTGAGCTACTTACGTTTAGTTATCATGGTTGGTCTCTATTTCAGCTCAGTGGAGGGACAAGCTCAATCTGCTTAGGGCTAGGAAGAATTTGGCGTAA

mRNA sequence

ATGTCCAAAAGGACAATATCTGCTAGTAGTGGGTTTGAGCCGTTACGTGACTTTGTTTTTGTGTTCCCTGTCAATTCTTCATCTTTTGCAGTGGAAATGCCAAGCGTTCTGCTCATGGCTGCTGTGCGCAATGTTCCAATTGCCAGACCAACCATGTATCAAGTGGTGAAAGAGATGATTGACAAGATGGGTTATGAAGTCAAACTTGTAAGGGTTACCAAGAGAGTGCATGAGGCATATTTTGCCCAGTTATACCTCTCAAAGATTGGTTGTGAGAATGACTGCCTCAGCTTTGATCTTCGACCTTCAGATGCAATAAACATTGCAGTCCGATGCAAGGTCCCAATTCAAGTGAACAAGTACCTTGCATACAGCGACGGTATGCGAGTTGTTGAAACTGGGAAGTTGTCAACCCAGACCCCTGCTACGGACGGTTTACTATTCACCGAACTGGATAGGCCCAGTGGTCAGCCTTGTGTGGAAGCCAAAGAGTTTGATCTGGTGAGGAATATGCTGATTGCTGCTGTTGAAGAGCGCTACAGAGATGCTGCTCAGTGGAGGGACAAGCTCAATCTGCTTAGGGCTAGGAAGAATTTGGCGTAA

Coding sequence (CDS)

ATGTCCAAAAGGACAATATCTGCTAGTAGTGGGTTTGAGCCGTTACGTGACTTTGTTTTTGTGTTCCCTGTCAATTCTTCATCTTTTGCAGTGGAAATGCCAAGCGTTCTGCTCATGGCTGCTGTGCGCAATGTTCCAATTGCCAGACCAACCATGTATCAAGTGGTGAAAGAGATGATTGACAAGATGGGTTATGAAGTCAAACTTGTAAGGGTTACCAAGAGAGTGCATGAGGCATATTTTGCCCAGTTATACCTCTCAAAGATTGGTTGTGAGAATGACTGCCTCAGCTTTGATCTTCGACCTTCAGATGCAATAAACATTGCAGTCCGATGCAAGGTCCCAATTCAAGTGAACAAGTACCTTGCATACAGCGACGGTATGCGAGTTGTTGAAACTGGGAAGTTGTCAACCCAGACCCCTGCTACGGACGGTTTACTATTCACCGAACTGGATAGGCCCAGTGGTCAGCCTTGTGTGGAAGCCAAAGAGTTTGATCTGGTGAGGAATATGCTGATTGCTGCTGTTGAAGAGCGCTACAGAGATGCTGCTCAGTGGAGGGACAAGCTCAATCTGCTTAGGGCTAGGAAGAATTTGGCGTAA

Protein sequence

MSKRTISASSGFEPLRDFVFVFPVNSSSFAVEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTELDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA
Homology
BLAST of CmaCh01G019290 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.5e-72
Identity = 132/169 (78.11%), Postives = 154/169 (91.12%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVK+M+DKMGYEV+LVRVT RVHEAYFA+LYLSK+G
Sbjct: 157 LEMPSVLLMAAMTNVQIARPTMYQVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVG 216

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
            ++DC+SFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV+++GKLS QTPA+DGLLFTE
Sbjct: 217 DKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTE 276

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
           LDRP+GQPC + KEFDLVRNM+ A  EERY +AA+WRDKL   +A++ L
Sbjct: 277 LDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKFQAKRKL 325

BLAST of CmaCh01G019290 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.0e-69
Identity = 126/169 (74.56%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVKEM+DKMGYEV+LVRVTKRVHEAYFAQL+LSK+G
Sbjct: 153 LEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVG 212

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
             ++C+SFDLRPSDAINIAVRCK+PIQVNKYLAYSDGMRV+E+GK+ST  PA+DGLLFTE
Sbjct: 213 NASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTE 272

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
            DRP+GQ C++ KEF+++  M+ A  EERY +AA+WRDKL   RA++NL
Sbjct: 273 QDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFRAKRNL 321

BLAST of CmaCh01G019290 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.1e-65
Identity = 125/171 (73.10%), Postives = 148/171 (86.55%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+RNV IARPT+YQVVKEMIDKMGYEVKLVR+ KR+ EAY A+L+L+K+G
Sbjct: 155 LEMPSVLLMAAIRNVHIARPTIYQVVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVG 214

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
              + ++FDLRPSDAINIAVRCKVPIQV++ LAYSDG+R VE  +++     +DGLLFTE
Sbjct: 215 DHTESITFDLRPSDAINIAVRCKVPIQVHRSLAYSDGIRSVEPARMAIAAGMSDGLLFTE 274

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRA-RKNLA 201
           LDRP GQPCVEA+EF L+RNMLIAAVEERY+DAA WRDKL LLR+ RKN A
Sbjct: 275 LDRPDGQPCVEAQEFGLIRNMLIAAVEERYKDAATWRDKLMLLRSKRKNWA 325

BLAST of CmaCh01G019290 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 7.6e-53
Identity = 100/167 (59.88%), Postives = 137/167 (82.04%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +E PS++L+AA+RN+ I RPT+Y VVKEM ++MGY V+LVR+T+ VH+AY+++LYL+KIG
Sbjct: 162 METPSIMLLAALRNIRIPRPTIYNVVKEMTERMGYAVRLVRITEMVHDAYYSRLYLAKIG 221

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
            E + +S DL+PSDAINIA RCKVPIQVN+ +AY++G++VV+    S    ++D   +T 
Sbjct: 222 NEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQPTP-SESYVSSDQFQYTR 281

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARK 198
           LDRP  QPC EA+EFDLVRNML+AAVEERY+DAAQ+RD+L + RA+K
Sbjct: 282 LDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFMFRAKK 327

BLAST of CmaCh01G019290 vs. ExPASy Swiss-Prot
Match: Q09LL3 (Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 7.6e-53
Identity = 100/167 (59.88%), Postives = 137/167 (82.04%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +E PS++L+AA+RN+ I RPT+Y VVKEM ++MGY V+LVR+T+ VH+AY+++LYL+KIG
Sbjct: 162 METPSIMLLAALRNIRIPRPTIYNVVKEMTERMGYAVRLVRITEMVHDAYYSRLYLAKIG 221

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
            E + +S DL+PSDAINIA RCKVPIQVN+ +AY++G++VV+    S    ++D   +T 
Sbjct: 222 NEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQPTP-SESYVSSDQFQYTR 281

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARK 198
           LDRP  QPC EA+EFDLVRNML+AAVEERY+DAAQ+RD+L + RA+K
Sbjct: 282 LDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFMFRAKK 327

BLAST of CmaCh01G019290 vs. ExPASy TrEMBL
Match: A0A6J1IXM9 (bifunctional nuclease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480857 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.2e-88
Identity = 169/170 (99.41%), Postives = 170/170 (100.00%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE
Sbjct: 218 CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA
Sbjct: 278 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 327

BLAST of CmaCh01G019290 vs. ExPASy TrEMBL
Match: A0A6J1FM73 (bifunctional nuclease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446486 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.3e-87
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           C+ DCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE
Sbjct: 218 CDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA
Sbjct: 278 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 327

BLAST of CmaCh01G019290 vs. ExPASy TrEMBL
Match: A0A1S3CRI0 (bifunctional nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103503917 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 7.1e-86
Identity = 163/170 (95.88%), Postives = 168/170 (98.82%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEM+DKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CE DCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVE+GKLSTQ PATDGLLFTE
Sbjct: 218 CETDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEF+LVRNMLIAAVEERYRDAAQWRDKLNLLRAR+NLA
Sbjct: 278 LDRPSGQPCVEAKEFNLVRNMLIAAVEERYRDAAQWRDKLNLLRARRNLA 327

BLAST of CmaCh01G019290 vs. ExPASy TrEMBL
Match: A0A5D3D874 (Bifunctional nuclease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G001050 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 7.1e-86
Identity = 163/170 (95.88%), Postives = 168/170 (98.82%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEM+DKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CE DCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVE+GKLSTQ PATDGLLFTE
Sbjct: 218 CETDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEF+LVRNMLIAAVEERYRDAAQWRDKLNLLRAR+NLA
Sbjct: 278 LDRPSGQPCVEAKEFNLVRNMLIAAVEERYRDAAQWRDKLNLLRARRNLA 327

BLAST of CmaCh01G019290 vs. ExPASy TrEMBL
Match: A0A6J1CCX9 (bifunctional nuclease 1 OS=Momordica charantia OX=3673 GN=LOC111010511 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.7e-85
Identity = 163/170 (95.88%), Postives = 167/170 (98.24%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLL+AAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLLAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLS Q PATDGLLFTE
Sbjct: 218 CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSIQAPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPC EAKEF+LVRNMLIAAVEERYRDAAQWRDKLNLLRAR+NLA
Sbjct: 278 LDRPSGQPCDEAKEFNLVRNMLIAAVEERYRDAAQWRDKLNLLRARRNLA 327

BLAST of CmaCh01G019290 vs. NCBI nr
Match: XP_022981828.1 (bifunctional nuclease 1-like [Cucurbita maxima] >XP_022981829.1 bifunctional nuclease 1-like [Cucurbita maxima] >XP_022981830.1 bifunctional nuclease 1-like [Cucurbita maxima] >XP_022981831.1 bifunctional nuclease 1-like [Cucurbita maxima])

HSP 1 Score: 335.9 bits (860), Expect = 2.4e-88
Identity = 169/170 (99.41%), Postives = 170/170 (100.00%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE
Sbjct: 218 CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA
Sbjct: 278 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 327

BLAST of CmaCh01G019290 vs. NCBI nr
Match: XP_022941089.1 (bifunctional nuclease 1-like [Cucurbita moschata])

HSP 1 Score: 332.4 bits (851), Expect = 2.7e-87
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           C+ DCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE
Sbjct: 218 CDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA
Sbjct: 278 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 327

BLAST of CmaCh01G019290 vs. NCBI nr
Match: XP_023525215.1 (bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525216.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525218.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525219.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.4 bits (851), Expect = 2.7e-87
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           C+ DCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE
Sbjct: 218 CDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA
Sbjct: 278 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 327

BLAST of CmaCh01G019290 vs. NCBI nr
Match: XP_008466529.1 (PREDICTED: bifunctional nuclease 1 [Cucumis melo] >XP_008466530.1 PREDICTED: bifunctional nuclease 1 [Cucumis melo] >KAA0041551.1 bifunctional nuclease 1 [Cucumis melo var. makuwa] >TYK19703.1 bifunctional nuclease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 326.6 bits (836), Expect = 1.5e-85
Identity = 163/170 (95.88%), Postives = 168/170 (98.82%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAAVRNVPIARPTMYQVVKEM+DKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLMAAVRNVPIARPTMYQVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CE DCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVE+GKLSTQ PATDGLLFTE
Sbjct: 218 CETDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPCVEAKEF+LVRNMLIAAVEERYRDAAQWRDKLNLLRAR+NLA
Sbjct: 278 LDRPSGQPCVEAKEFNLVRNMLIAAVEERYRDAAQWRDKLNLLRARRNLA 327

BLAST of CmaCh01G019290 vs. NCBI nr
Match: XP_022139665.1 (bifunctional nuclease 1 [Momordica charantia] >XP_022139672.1 bifunctional nuclease 1 [Momordica charantia] >XP_022139679.1 bifunctional nuclease 1 [Momordica charantia] >XP_022139685.1 bifunctional nuclease 1 [Momordica charantia] >XP_022139693.1 bifunctional nuclease 1 [Momordica charantia])

HSP 1 Score: 324.7 bits (831), Expect = 5.6e-85
Identity = 163/170 (95.88%), Postives = 167/170 (98.24%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLL+AAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG
Sbjct: 158 LEMPSVLLLAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 217

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
           CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLS Q PATDGLLFTE
Sbjct: 218 CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSIQAPATDGLLFTE 277

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNLA 201
           LDRPSGQPC EAKEF+LVRNMLIAAVEERYRDAAQWRDKLNLLRAR+NLA
Sbjct: 278 LDRPSGQPCDEAKEFNLVRNMLIAAVEERYRDAAQWRDKLNLLRARRNLA 327

BLAST of CmaCh01G019290 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.0e-73
Identity = 132/169 (78.11%), Postives = 154/169 (91.12%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVK+M+DKMGYEV+LVRVT RVHEAYFA+LYLSK+G
Sbjct: 157 LEMPSVLLMAAMTNVQIARPTMYQVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVG 216

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
            ++DC+SFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV+++GKLS QTPA+DGLLFTE
Sbjct: 217 DKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTE 276

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
           LDRP+GQPC + KEFDLVRNM+ A  EERY +AA+WRDKL   +A++ L
Sbjct: 277 LDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKFQAKRKL 325

BLAST of CmaCh01G019290 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.0e-73
Identity = 132/169 (78.11%), Postives = 154/169 (91.12%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVK+M+DKMGYEV+LVRVT RVHEAYFA+LYLSK+G
Sbjct: 157 LEMPSVLLMAAMTNVQIARPTMYQVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVG 216

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
            ++DC+SFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV+++GKLS QTPA+DGLLFTE
Sbjct: 217 DKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTE 276

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
           LDRP+GQPC + KEFDLVRNM+ A  EERY +AA+WRDKL   +A++ L
Sbjct: 277 LDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKFQAKRKL 325

BLAST of CmaCh01G019290 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-70
Identity = 126/169 (74.56%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVKEM+DKMGYEV+LVRVTKRVHEAYFAQL+LSK+G
Sbjct: 153 LEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVG 212

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
             ++C+SFDLRPSDAINIAVRCK+PIQVNKYLAYSDGMRV+E+GK+ST  PA+DGLLFTE
Sbjct: 213 NASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTE 272

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
            DRP+GQ C++ KEF+++  M+ A  EERY +AA+WRDKL   RA++NL
Sbjct: 273 QDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFRAKRNL 321

BLAST of CmaCh01G019290 vs. TAIR 10
Match: AT1G75380.2 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-70
Identity = 126/169 (74.56%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVKEM+DKMGYEV+LVRVTKRVHEAYFAQL+LSK+G
Sbjct: 153 LEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVG 212

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
             ++C+SFDLRPSDAINIAVRCK+PIQVNKYLAYSDGMRV+E+GK+ST  PA+DGLLFTE
Sbjct: 213 NASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTE 272

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
            DRP+GQ C++ KEF+++  M+ A  EERY +AA+WRDKL   RA++NL
Sbjct: 273 QDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFRAKRNL 321

BLAST of CmaCh01G019290 vs. TAIR 10
Match: AT1G75380.3 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-70
Identity = 126/169 (74.56%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 31  VEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIG 90
           +EMPSVLLMAA+ NV IARPTMYQVVKEM+DKMGYEV+LVRVTKRVHEAYFAQL+LSK+G
Sbjct: 153 LEMPSVLLMAAMTNVQIARPTMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVG 212

Query: 91  CENDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTE 150
             ++C+SFDLRPSDAINIAVRCK+PIQVNKYLAYSDGMRV+E+GK+ST  PA+DGLLFTE
Sbjct: 213 NASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTE 272

Query: 151 LDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKLNLLRARKNL 200
            DRP+GQ C++ KEF+++  M+ A  EERY +AA+WRDKL   RA++NL
Sbjct: 273 QDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDKLGQFRAKRNL 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VH21.5e-7278.11Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1[more]
Q9FWS62.0e-6974.56Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1[more]
Q6YZM65.1e-6573.10Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV... [more]
Q5N8J37.6e-5359.88Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV... [more]
Q09LL37.6e-5359.88Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IXM91.2e-8899.41bifunctional nuclease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480857 PE=3 SV... [more]
A0A6J1FM731.3e-8798.24bifunctional nuclease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446486 PE=3 ... [more]
A0A1S3CRI07.1e-8695.88bifunctional nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103503917 PE=3 SV=1[more]
A0A5D3D8747.1e-8695.88Bifunctional nuclease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1CCX92.7e-8595.88bifunctional nuclease 1 OS=Momordica charantia OX=3673 GN=LOC111010511 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022981828.12.4e-8899.41bifunctional nuclease 1-like [Cucurbita maxima] >XP_022981829.1 bifunctional nuc... [more]
XP_022941089.12.7e-8798.24bifunctional nuclease 1-like [Cucurbita moschata][more]
XP_023525215.12.7e-8798.24bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525216.1 bifunc... [more]
XP_008466529.11.5e-8595.88PREDICTED: bifunctional nuclease 1 [Cucumis melo] >XP_008466530.1 PREDICTED: bif... [more]
XP_022139665.15.6e-8595.88bifunctional nuclease 1 [Momordica charantia] >XP_022139672.1 bifunctional nucle... [more]
Match NameE-valueIdentityDescription
AT1G19660.11.0e-7378.11Wound-responsive family protein [more]
AT1G19660.21.0e-7378.11Wound-responsive family protein [more]
AT1G75380.11.4e-7074.56bifunctional nuclease in basal defense response 1 [more]
AT1G75380.21.4e-7074.56bifunctional nuclease in basal defense response 1 [more]
AT1G75380.31.4e-7074.56bifunctional nuclease in basal defense response 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003729Bifunctional nuclease domainPFAMPF02577DUF151coord: 38..125
e-value: 9.5E-17
score: 60.8
IPR003729Bifunctional nuclease domainPROSITEPS51658BFNcoord: 1..130
score: 25.15608
IPR036104Bifunctional nuclease superfamilyGENE3D3.10.690.10Bifunctional nuclease domaincoord: 31..136
e-value: 8.4E-28
score: 98.7
IPR036104Bifunctional nuclease superfamilySUPERFAMILY103256Hypothetical protein TM0160coord: 32..134
NoneNo IPR availablePANTHERPTHR15160:SF3BIFUNCTIONAL NUCLEASE 1coord: 31..199
NoneNo IPR availablePANTHERPTHR15160VON HIPPEL-LINDAU PROTEINcoord: 31..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G019290.1CmaCh01G019290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005634 nucleus
cellular_component GO:0030891 VCB complex
molecular_function GO:0004518 nuclease activity
molecular_function GO:0046983 protein dimerization activity