CmaCh01G014110 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G014110
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSerine hydroxymethyltransferase
LocationCma_Chr01: 9966020 .. 9970659 (+)
RNA-Seq ExpressionCmaCh01G014110
SyntenyCmaCh01G014110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTCTTCACTGGAATGCTCTTCTCCGATTAAACTGCATTTCGTTATTTTATTATAAATCTGTTTCGTTTTCATTTTTCATCTCTATTTCTATCTGAATCCGGATTTCCGCTCCTTTGATGGAAGCTTCTTCTTCGTCTACAACCACTCCTCGCCGGTCGTTCTTCCGATACTGTCACTGTAGTTTTTTCAGGCGTTGTTGTCGCTGTTTCTGCTGTTTCTACTCCTATCCTTAGGTGTTCGTTGAATTCGTTTTGTTCATCGTCTTCTTCTGCTGCTGATTTTCGGGATTATGGATTTTCGCCATGCCCAGTCGGATCTTTCTTTACGATTTAGTTCTCATGCCACATCTTCGATATCTAGAAGCTCGATTGGGGATAACTCGAGCTATTTGTGTTCGACGGCGTCGAATTCGTCGTACTTGTTAGAACTCCGTGAGCAAAAGGACGAGAAATTCAATTTTGTGAAAGGGGACCCGGATGATCATAGCGATGATGAGAAGTTCAGCCTTCTAGGGCAACCCTTGCGTGTGAAGAGGCAGAGAGACAGTCAATCGTTTCTCAACCAGGATTCTGCGAAACGGTTAGCGATTATGGATGAATCGAGCCTTGAGGCTAGACGAGCGTCGGTTAGGGCATGGGGGAATCAGTCGCTTCGAATTGCCGACCCGGAAATTCATGATATTATGGCGAAGGAGAAGCAGAGACAATATAGGGGTATTGAATTGGTTGCTTCGGAGAATTTTGTGTGCAGGGCTGTTATGGAAGCCCTAGGTAGCCATTTGACGAATAAGTACTCTGAGGGAATGCCTGGCGCTAGATACTATACGGGTAATCAGCACATAGATGAGATTGAATTGCTTTGTTGTGAGAGAGCGTTGGCCGCTTTTGATCTAGATCCTGAGAAATGGGGAGTGAATGTGCAGCCTTACTCTTGTACATCAGCAAACTTCGCTGTGTACACCGGTCTTTTGCACCCAAAGGATCGAATTATGGGTTTGGATTCAGCATCGGGAGGACATCTTAGTCATGGGTATTATACTCCGGGTGGGAAAAAGATTTCAGCGGCTTCTATATTCTTTCAAAGTCTACCTTATAAAGTGAATCCCCTAACTGGGTACGTTGATTATGATAAGCTTGAAGAGAAGGCGCTTGATTATCGTCCGAAAATACTAATCTGTGGAGGAAGCTCGTATCCACGCGAATGGGACTATGGTAGATGTAGGCAGATCGCAGACAAATGCGGAGCAATTCTGATGTCCGACATGTCCCATATAAGCGGTCTTGTGGCGGCCAAGGTGTGTGGATTCTTCTTATAACATGTTTCTTTAGAATTGTGATTGAATTCATCATGTCAGATTTTAAGATTTCTGTAAGATAATGCCTTTTCTTATTGTATTTGCCAGCATTAATTTTAATTTGTTATGTTAAAGTCTGATTGGGGTGGGATTAGTTTAGCCATTAGGATAGCGATTTGAACCTTCCTGCCATTTTTGCTACTCATTTATGTCCTTATGACTTTGTCTCTGCTGCAGGAATGTGTTAGTCCGTTTCAATATTGTGATATAGTAACTTCAACAACACACAAAAGTCTCAGAGGGCCCAGAGGAGGTATTATCTTTTTCAGGAGGGGTCTAAAATCGAGGAAGCAAGGCGTGCTTCTAAATCATGGAGATGAAACTGGTACTTATGACTTTGAGGACAGGATAAACTTTTCCGTTTTCCCGTCGTTACAAGGGGGTCCCCATAATAATCATATTGCTGCACTCGCCATAGCGTTAAAGCAAGTGGCTTCTCTTGAGTACAGAGTATATATTCGACAGGTGAAGAAAAATGCGCAGGCTTTAGCATCAGCTTTATTGAGAAGAAAATGTAGATTGGTAACTGATGGTACAGACAATCATTTGGTGCTGTGGGATCTTATTCCTTTGGGACTAACAGGTACTGCACTCAGATATTTGCCTCCTTCTGGATATAAATTGTTTAATTGCACACTAGATTTTAATGGTTCTCTGATATGATAAGACCCTTGAGCAAATATTGTTAGATATACTAACTTTTGGTGATTATTTTTGTGCTTCCTGAGCTGTCATTAATCGTTTATGCTGGATTATTGAGTTAGCTTTTATGGGTTATCTCTGGGTTTGATTTGATTGCCTTGTCCCTAAGACATAAGACGTAAGGGCTAATAGCATAGCTGTTCCCCTCTTATGTAACCTGGCTTCTCATGCCATGTAATCGTAGGTTCATTGCTTCTCAATTATTGGGGATGTCCACTTGAGTTATTCTTTGAGAAAGAAATTTCTAACCTTCTTTCCTCTTTATTTATAATTCATGTTATTTGTAAAAGCTCAAACCCACTAGCAGATATTGTCTTCTTTGGACTTCCCCTTAGGGTTTTTAAAACGCTTTTGTCATGGAGAGGTTTTCACATGCTTATAAAAATGTTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTTCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCTTCGTTCCTCTCTCCAACTGATGTGAGATCTCACAATCCACCCCCTTCGTTCCTTTCTTCAATCAATGTGGGATCTCACACAGCTCATCGCCATAGTAGGGTTCAAAAAATGTGATTTTCACTCTAATTAGTATTAATTTCTACTTTAACCTTCTATGGATTTTCAAGTCCGCAGATGAGGAAGTGCTTAAAGTATTATATATATGATCGAATTTGTCATAGCTTATCAACTTTAAGCTTTTAAATTGAGGGGTGGCTCTGCTAATTCTTATACTTTTGTTGTTTATTTTATTTTGCAGCCAAGAACTATGAGAAGATCTGTGAGCTATGTCATATTACAGTGAACAAGTGTGCCATCTATGGTGACAATGGAGCCATTTCTCCTGGAGGAGTGAGAATAGGTGCGTACTTGTATATAGAGTACTCACTCAGCTGATTTTGCTCTTGAGATAAACTTATAGAAACTTATTTTCTTGTTCAGGCACTCCTGCAATGACAACCAGAGGTTGCCTAGAGGCTGATTTCGAGACGATTGCTGAGATCCTTCTTAAAGCAGTTCAGATCACAAAAATGGTCCTTCATAGGGAACACGGAAAACTACATAAAGATATAATGAAGAATTTACAAAACAACAAAGATATTATGGAGCTTCGAAACCGCGTTGAGGCCTTTGCTGCTTCATTCGCAATGCCAGGATTCGATATATGAATGTATGAGCCTCTGCTTTTACACCACAGCTTCACTTGGTAACCTCAAACACGAACAAATTCATGCCCCCCCACTTGTTTGCCTGTAAATTATAGAAAAAAAGGTTGAAGATAGACATGAATGAATCATTCATATACTTGTAACTGTGAATCCCAGATTACAATAGGGAGGTTTTTACCTCAAATATCTCCCATGTTTGGTGCCACTGTTTTGTTTTGTTTTGTTCGAGTATGTTTAATCATCACTGTTCATATACCTAATATTGGTACTTCGAAGAAAAAGAGAGAAAAAAAAGGAGGAAGCTTTTGATGCAAGCAAAATCTACTTTTGGAACATTATAGTGGGGTTTTACCTTTTATTATGAAGATGATGACCCCC

mRNA sequence

TTCTCTTCACTGGAATGCTCTTCTCCGATTAAACTGCATTTCGTTATTTTATTATAAATCTGTTTCGTTTTCATTTTTCATCTCTATTTCTATCTGAATCCGGATTTCCGCTCCTTTGATGGAAGCTTCTTCTTCGTCTACAACCACTCCTCGCCGGTCGTTCTTCCGATACTGTCACTGTAGTTTTTTCAGGCGTTGTTGTCGCTGTTTCTGCTGTTTCTACTCCTATCCTTAGGTGTTCGTTGAATTCGTTTTGTTCATCGTCTTCTTCTGCTGCTGATTTTCGGGATTATGGATTTTCGCCATGCCCAGTCGGATCTTTCTTTACGATTTAGTTCTCATGCCACATCTTCGATATCTAGAAGCTCGATTGGGGATAACTCGAGCTATTTGTGTTCGACGGCGTCGAATTCGTCGTACTTGTTAGAACTCCGTGAGCAAAAGGACGAGAAATTCAATTTTGTGAAAGGGGACCCGGATGATCATAGCGATGATGAGAAGTTCAGCCTTCTAGGGCAACCCTTGCGTGTGAAGAGGCAGAGAGACAGTCAATCGTTTCTCAACCAGGATTCTGCGAAACGGTTAGCGATTATGGATGAATCGAGCCTTGAGGCTAGACGAGCGTCGGTTAGGGCATGGGGGAATCAGTCGCTTCGAATTGCCGACCCGGAAATTCATGATATTATGGCGAAGGAGAAGCAGAGACAATATAGGGGTATTGAATTGGTTGCTTCGGAGAATTTTGTGTGCAGGGCTGTTATGGAAGCCCTAGGTAGCCATTTGACGAATAAGTACTCTGAGGGAATGCCTGGCGCTAGATACTATACGGGTAATCAGCACATAGATGAGATTGAATTGCTTTGTTGTGAGAGAGCGTTGGCCGCTTTTGATCTAGATCCTGAGAAATGGGGAGTGAATGTGCAGCCTTACTCTTGTACATCAGCAAACTTCGCTGTGTACACCGGTCTTTTGCACCCAAAGGATCGAATTATGGGTTTGGATTCAGCATCGGGAGGACATCTTAGTCATGGGTATTATACTCCGGGTGGGAAAAAGATTTCAGCGGCTTCTATATTCTTTCAAAGTCTACCTTATAAAGTGAATCCCCTAACTGGGTACGTTGATTATGATAAGCTTGAAGAGAAGGCGCTTGATTATCGTCCGAAAATACTAATCTGTGGAGGAAGCTCGTATCCACGCGAATGGGACTATGGTAGATGTAGGCAGATCGCAGACAAATGCGGAGCAATTCTGATGTCCGACATGTCCCATATAAGCGGTCTTGTGGCGGCCAAGGAATGTGTTAGTCCGTTTCAATATTGTGATATAGTAACTTCAACAACACACAAAAGTCTCAGAGGGCCCAGAGGAGGTATTATCTTTTTCAGGAGGGGTCTAAAATCGAGGAAGCAAGGCGTGCTTCTAAATCATGGAGATGAAACTGGTACTTATGACTTTGAGGACAGGATAAACTTTTCCGTTTTCCCGTCGTTACAAGGGGGTCCCCATAATAATCATATTGCTGCACTCGCCATAGCGTTAAAGCAAGTGGCTTCTCTTGAGTACAGAGTATATATTCGACAGGTGAAGAAAAATGCGCAGGCTTTAGCATCAGCTTTATTGAGAAGAAAATGTAGATTGGTAACTGATGGTACAGACAATCATTTGGTGCTGTGGGATCTTATTCCTTTGGGACTAACAGCCAAGAACTATGAGAAGATCTGTGAGCTATGTCATATTACAGTGAACAAGTGTGCCATCTATGGTGACAATGGAGCCATTTCTCCTGGAGGAGTGAGAATAGGCACTCCTGCAATGACAACCAGAGGTTGCCTAGAGGCTGATTTCGAGACGATTGCTGAGATCCTTCTTAAAGCAGTTCAGATCACAAAAATGGTCCTTCATAGGGAACACGGAAAACTACATAAAGATATAATGAAGAATTTACAAAACAACAAAGATATTATGGAGCTTCGAAACCGCGTTGAGGCCTTTGCTGCTTCATTCGCAATGCCAGGATTCGATATATGAATGTATGAGCCTCTGCTTTTACACCACAGCTTCACTTGGTAACCTCAAACACGAACAAATTCATGCCCCCCCACTTGTTTGCCTGTAAATTATAGAAAAAAAGGTTGAAGATAGACATGAATGAATCATTCATATACTTGTAACTGTGAATCCCAGATTACAATAGGGAGGTTTTTACCTCAAATATCTCCCATGTTTGGTGCCACTGTTTTGTTTTGTTTTGTTCGAGTATGTTTAATCATCACTGTTCATATACCTAATATTGGTACTTCGAAGAAAAAGAGAGAAAAAAAAGGAGGAAGCTTTTGATGCAAGCAAAATCTACTTTTGGAACATTATAGTGGGGTTTTACCTTTTATTATGAAGATGATGACCCCC

Coding sequence (CDS)

ATGGATTTTCGCCATGCCCAGTCGGATCTTTCTTTACGATTTAGTTCTCATGCCACATCTTCGATATCTAGAAGCTCGATTGGGGATAACTCGAGCTATTTGTGTTCGACGGCGTCGAATTCGTCGTACTTGTTAGAACTCCGTGAGCAAAAGGACGAGAAATTCAATTTTGTGAAAGGGGACCCGGATGATCATAGCGATGATGAGAAGTTCAGCCTTCTAGGGCAACCCTTGCGTGTGAAGAGGCAGAGAGACAGTCAATCGTTTCTCAACCAGGATTCTGCGAAACGGTTAGCGATTATGGATGAATCGAGCCTTGAGGCTAGACGAGCGTCGGTTAGGGCATGGGGGAATCAGTCGCTTCGAATTGCCGACCCGGAAATTCATGATATTATGGCGAAGGAGAAGCAGAGACAATATAGGGGTATTGAATTGGTTGCTTCGGAGAATTTTGTGTGCAGGGCTGTTATGGAAGCCCTAGGTAGCCATTTGACGAATAAGTACTCTGAGGGAATGCCTGGCGCTAGATACTATACGGGTAATCAGCACATAGATGAGATTGAATTGCTTTGTTGTGAGAGAGCGTTGGCCGCTTTTGATCTAGATCCTGAGAAATGGGGAGTGAATGTGCAGCCTTACTCTTGTACATCAGCAAACTTCGCTGTGTACACCGGTCTTTTGCACCCAAAGGATCGAATTATGGGTTTGGATTCAGCATCGGGAGGACATCTTAGTCATGGGTATTATACTCCGGGTGGGAAAAAGATTTCAGCGGCTTCTATATTCTTTCAAAGTCTACCTTATAAAGTGAATCCCCTAACTGGGTACGTTGATTATGATAAGCTTGAAGAGAAGGCGCTTGATTATCGTCCGAAAATACTAATCTGTGGAGGAAGCTCGTATCCACGCGAATGGGACTATGGTAGATGTAGGCAGATCGCAGACAAATGCGGAGCAATTCTGATGTCCGACATGTCCCATATAAGCGGTCTTGTGGCGGCCAAGGAATGTGTTAGTCCGTTTCAATATTGTGATATAGTAACTTCAACAACACACAAAAGTCTCAGAGGGCCCAGAGGAGGTATTATCTTTTTCAGGAGGGGTCTAAAATCGAGGAAGCAAGGCGTGCTTCTAAATCATGGAGATGAAACTGGTACTTATGACTTTGAGGACAGGATAAACTTTTCCGTTTTCCCGTCGTTACAAGGGGGTCCCCATAATAATCATATTGCTGCACTCGCCATAGCGTTAAAGCAAGTGGCTTCTCTTGAGTACAGAGTATATATTCGACAGGTGAAGAAAAATGCGCAGGCTTTAGCATCAGCTTTATTGAGAAGAAAATGTAGATTGGTAACTGATGGTACAGACAATCATTTGGTGCTGTGGGATCTTATTCCTTTGGGACTAACAGCCAAGAACTATGAGAAGATCTGTGAGCTATGTCATATTACAGTGAACAAGTGTGCCATCTATGGTGACAATGGAGCCATTTCTCCTGGAGGAGTGAGAATAGGCACTCCTGCAATGACAACCAGAGGTTGCCTAGAGGCTGATTTCGAGACGATTGCTGAGATCCTTCTTAAAGCAGTTCAGATCACAAAAATGGTCCTTCATAGGGAACACGGAAAACTACATAAAGATATAATGAAGAATTTACAAAACAACAAAGATATTATGGAGCTTCGAAACCGCGTTGAGGCCTTTGCTGCTTCATTCGCAATGCCAGGATTCGATATATGA

Protein sequence

MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Homology
BLAST of CmaCh01G014110 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 7.0e-232
Identity = 403/592 (68.07%), Postives = 471/592 (79.56%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFS-SHAT---SSISRSSIGDNS-------SYLCSTASNSSYLLELRE 60
           MD   +Q++  L F  SHA+   +   R+ I D+S       S+     + S   L+L E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  QKDEKFNFVK------GDPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDE 120
           QK EK   V       G   D  +DE F +LG  + +KRQRD    L Q    + + + +
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGD 120

Query: 121 SSLEARRASVRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSH 180
           S LE+RRA+VRAWG+Q + +ADP+IH++M KEKQRQ RGIEL+ASENFVCRAVMEALGSH
Sbjct: 121 SDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSH 180

Query: 181 LTNKYSEGMPGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVY 240
           LTNKYSEGMPGARYYTGNQ+ID+IE LC ERAL AF L+ +KWGVNVQPYSCTSANFAVY
Sbjct: 181 LTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVY 240

Query: 241 TGLLHPKDRIMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLE 300
           TGLL P +RIMGLDS SGGH+SHGY TPGGKKISAASIFF+S PYKVNP TGY+DYDKLE
Sbjct: 241 TGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLE 300

Query: 301 EKALDYRPKILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQY 360
           +KALDYRPKILICGGSSYPR+WD+ R RQIADKCGA+LM DM+HISGLVA KEC +PF +
Sbjct: 301 DKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDH 360

Query: 361 CDIVTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQG 420
           CDIVTSTTHK LRGPRGGIIF+RRG K RKQG   +H D +  YD E++INF+VFPSLQG
Sbjct: 361 CDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQG 420

Query: 421 GPHNNHIAALAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWD 480
           GPHNNHIAALAIALKQVA+ EY+ YI+Q+KKNAQALA+ALLRRKCRLVT GTDNHL+LWD
Sbjct: 421 GPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWD 480

Query: 481 LIPLGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIA 540
           L P+GLT K YEK+CE+CHIT+NK AI+GDNG ISPGGVRIGTPAMTTRGC+E+DFET+A
Sbjct: 481 LTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMA 540

Query: 541 EILLKAVQITKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMP 576
           + L+KA QIT   L REHGK HK+ +K+L  NKDI ELRNRVEAFA  + MP
Sbjct: 541 DFLIKAAQITS-ALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of CmaCh01G014110 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 8.8e-227
Identity = 400/598 (66.89%), Postives = 474/598 (79.26%), Query Frame = 0

Query: 4   RHAQSDLSLRF-SSHA--TSSISRSSIGD-------NSSYLCSTASNSSYLLELREQK-- 63
           R AQSDLSL F SSHA       R  I D       +SS+  S+       L+L EQ+  
Sbjct: 3   RIAQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFD 62

Query: 64  --------DEKFNFVKG--DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIM 123
                    E+ + V G  D DD  ++E+F LLG P+++KR R   S+    S+     +
Sbjct: 63  VTGSCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFV 122

Query: 124 DESSLEARRASVRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALG 183
            +S +E+RRA VRAWGNQS+  ADPEIH+ M KEKQRQ+RGIEL+ASENFVCRAVMEALG
Sbjct: 123 VDSGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALG 182

Query: 184 SHLTNKYSEGMPGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFA 243
           SHLTNKYSEGMPGARYYTGNQ+ID+IE+LC ERALAAF L+ EKWGVNVQPYSCTSANFA
Sbjct: 183 SHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFA 242

Query: 244 VYTGLLHPKDRIMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDK 303
           V+TGLL P +RIMGLDS SGGH+SHGYYTPGGKK+S ASIFF+S PYKV+P TGY+DYDK
Sbjct: 243 VFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDK 302

Query: 304 LEEKALDYRPKILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPF 363
           LEEKALDYRPKILICGGSSYPR+W++ R R IADKCGA+LM DM+ ISGLVAAKE  +PF
Sbjct: 303 LEEKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPF 362

Query: 364 QYCDIVTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSL 423
            YCDIVTSTTHKSLRGPRGGIIF++RGLK +KQ + LNH +    YDFE++INFSVFPSL
Sbjct: 363 DYCDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSL 422

Query: 424 QGGPHNNHIAALAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVL 483
           QGGPHNNHIAALAIALKQ AS EY++Y+RQVKKNA+ALASAL+ RKC+L+T GTDNHL+L
Sbjct: 423 QGGPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLL 482

Query: 484 WDLIPLGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFET 543
           WDL PLGLT K YEK+CE+CHITVNK AI+ +NG ISPGGVRIG+PAMT+RGCLE +FET
Sbjct: 483 WDLTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFET 542

Query: 544 IAEILLKAVQITKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           +A+ L +A QI      REHGKL K+ +K++ + K+I +LRN+VEAFA  FAMP FD+
Sbjct: 543 MADFLYRAAQIAS-AAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of CmaCh01G014110 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.3e-177
Identity = 300/465 (64.52%), Postives = 366/465 (78.71%), Query Frame = 0

Query: 113 VRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN SL   DPEIHD++ KEK+RQ RGIEL+ASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 173 PGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDR 232
           PG RYY GN+ IDEIE LC  RAL AF  DP  WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGHL+HGYYT GGKKISA SI+F+SLPYKVN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 293 ILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTH 352
           +LICGGS+YPR+WDY R R IADK GA+L+ DM+HISGLVAA+E  +PF+YCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAA 412
           KSLRGPR G+IF+R+G K  K+G       E   YDFED+INF+VFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 413 LAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAK 472
           LA+ALKQ  +  ++VY +QVK NA AL + L+ +  ++VT+GT+NHLVLWDL PLGLT  
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 473 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQI 532
             EK+C+LC IT+NK A++GD+ A++PGGVRIG PAMT+RG +E DFE I E L +AV +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 533 TKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGF 578
           T + + + +GKL KD  K L NNKD+ +L+  VE F+AS+ MPGF
Sbjct: 424 T-LDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of CmaCh01G014110 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 3.1e-171
Identity = 289/467 (61.88%), Postives = 355/467 (76.02%), Query Frame = 0

Query: 113 VRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  L   DPEI+D++ KEK RQ RGIEL+A+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 173 PGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDR 232
           PG RYY G + IDEIE LC  R+L AF  +PEKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGH++HGYY+ GGK ISA SI+F++LPYKV+  TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 293 ILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTH 352
           ++ICGG+SYPREWDY R R +ADK GA L+ DM+H S LVAA+E   PF+YCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAA 412
           KSLRGPR G+IF+R+G K  K+G       E   YDF+ +IN +VFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 413 LAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAK 472
           LA+ALKQV +  ++VY +QVK NA  LAS L+ +   LVTDGTDNHL+LWDL PLGLT  
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 473 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQI 532
             EK+CELC+IT+N+ A++GD   ++PGGVRIGTPAMT+RG +E DFE I E L +AV I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 533 TKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           T + +  ++GK+ KD  K L NNK+I E++  VE F   F MPGF I
Sbjct: 424 T-LDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of CmaCh01G014110 vs. ExPASy Swiss-Prot
Match: Q54EW1 (Serine hydroxymethyltransferase 2 OS=Dictyostelium discoideum OX=44689 GN=shmt2 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.4e-152
Identity = 256/459 (55.77%), Postives = 345/459 (75.16%), Query Frame = 0

Query: 118 NQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARY 177
           N+S+  +DPEI+D+M KEKQRQ+ G+EL+ASENF  RAVME++GS  TNKY+EG+PGARY
Sbjct: 31  NRSVSESDPEIYDLMMKEKQRQFTGLELIASENFTSRAVMESIGSCFTNKYAEGLPGARY 90

Query: 178 YTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLD 237
           Y GN+ +D++E LC +RAL  F+L+PE+WGVNVQPYS ++ANFA +TGLL P DRIMGLD
Sbjct: 91  YGGNEVVDQLENLCIKRALETFNLNPEEWGVNVQPYSGSTANFAAFTGLLKPHDRIMGLD 150

Query: 238 SASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICG 297
             SGGHL+HGY T   KKISA SIFF+S+PY+VN  TGYVDY+K+E  A  +RPK+LI G
Sbjct: 151 LPSGGHLTHGYQT-DKKKISATSIFFESMPYQVNE-TGYVDYNKMEANAALFRPKLLIAG 210

Query: 298 GSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRG 357
            S+YPREWDY R R+IADK GA L+ DM+HISG+VA K+ +SPF +CD+VT+TTHK+LRG
Sbjct: 211 ASAYPREWDYERMRKIADKHGAYLLCDMAHISGMVAGKQAISPFLFCDVVTTTTHKTLRG 270

Query: 358 PRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIAL 417
           PR G+IFFR+  +   +G +++        D E+RINF+VFPS QGGPH N IA +A+AL
Sbjct: 271 PRAGLIFFRKTKRRDAKGNIIDD-------DLENRINFAVFPSCQGGPHENTIAGIAVAL 330

Query: 418 KQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKI 477
           K+ +S +++ Y +QV++N+Q +   L +R   LVT+GTDNHLVLWDL P G+T    EK 
Sbjct: 331 KEASSPDFQEYTKQVRRNSQTMGEELKKRGYSLVTEGTDNHLVLWDLRPQGITGSKIEKA 390

Query: 478 CELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVL 537
           C+  HITVNK A+YGD  AI+PGGVR+G PA+T+RG  E DF  + + L + V+I+ + +
Sbjct: 391 CDEAHITVNKNAVYGDTNAIAPGGVRLGAPALTSRGLKEQDFVKVVDFLDRVVKIS-LDI 450

Query: 538 HREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPG 577
             + GK   D  + + +N+D+ ++R  V+ F+  F MPG
Sbjct: 451 QSKVGKKMPDFQRAIADNQDLKQIRQEVKEFSTKFGMPG 479

BLAST of CmaCh01G014110 vs. ExPASy TrEMBL
Match: A0A6J1IY33 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480261 PE=3 SV=1)

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
           ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Sbjct: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of CmaCh01G014110 vs. ExPASy TrEMBL
Match: A0A6J1FSB1 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446415 PE=3 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 572/579 (98.79%), Postives = 576/579 (99.48%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPDDHSDDE+FSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLE RRASVRAWGNQS
Sbjct: 61  DPDDHSDDEQFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEVRRASVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKI+ICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKIVICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHG+ETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
            SLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 TSLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKLHKD+MKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Sbjct: 541 HGKLHKDMMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of CmaCh01G014110 vs. ExPASy TrEMBL
Match: A0A6J1C598 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111008604 PE=3 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.3e-309
Identity = 521/571 (91.24%), Postives = 547/571 (95.80%), Query Frame = 0

Query: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDD 68
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61

Query: 69  EKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQSLRIADPEI 128
           EKFSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLEARRASVRAWGNQSLR+ADPEI
Sbjct: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121

Query: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181

Query: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSASGGHLSHGY 248
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDR+MGLDSASGGHLSHGY
Sbjct: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241

Query: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYG 308
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 
Sbjct: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301

Query: 309 RCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRGGIIFFRRG 368
           R RQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361

Query: 369 LKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASLEYRVY 428
           LKSRKQGVLL HGD+T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQVAS EY VY
Sbjct: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421

Query: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481

Query: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDI 548
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKDI
Sbjct: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541

Query: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572

BLAST of CmaCh01G014110 vs. ExPASy TrEMBL
Match: A0A5A7VB49 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001500 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.2e-298
Identity = 512/582 (87.97%), Postives = 538/582 (92.44%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  + R+QKDEKF+ VKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
             DD SDDEKFSLLGQPLRVKRQRD+ SFL+QDSAKR A+ DE+SLE RRASV+ WGNQS
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIH+IM KEK+RQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDE--TGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL HGD+    TYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKIC 480
           QVAS EYRVYIRQVKKNAQALASALLRR CRLVT+GTDNHL+LWDL PLGLTAKNYE IC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 ELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLH 540
           E CHITVNK AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKA  ITK+V+ 
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 R-EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           R + GKLHKDIMKNLQNNKDI+ELRNRVE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of CmaCh01G014110 vs. ExPASy TrEMBL
Match: A0A1S3B9K5 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103487552 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.2e-298
Identity = 512/582 (87.97%), Postives = 538/582 (92.44%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  + R+QKDEKF+ VKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
             DD SDDEKFSLLGQPLRVKRQRD+ SFL+QDSAKR A+ DE+SLE RRASV+ WGNQS
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIH+IM KEK+RQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDE--TGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL HGD+    TYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKIC 480
           QVAS EYRVYIRQVKKNAQALASALLRR CRLVT+GTDNHL+LWDL PLGLTAKNYE IC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 ELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLH 540
           E CHITVNK AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKA  ITK+V+ 
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 R-EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           R + GKLHKDIMKNLQNNKDI+ELRNRVE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of CmaCh01G014110 vs. NCBI nr
Match: XP_022980970.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
           ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Sbjct: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of CmaCh01G014110 vs. NCBI nr
Match: KAG7031702.1 (Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 575/579 (99.31%), Postives = 577/579 (99.65%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKEC SPFQYCD+VTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECASPFQYCDVVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHG+ETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
           ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Sbjct: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of CmaCh01G014110 vs. NCBI nr
Match: KAG6608069.1 (Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 574/579 (99.14%), Postives = 576/579 (99.48%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPD HSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS
Sbjct: 61  DPDFHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKEC SPFQYCD+VTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECASPFQYCDVVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHG+ETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
           ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Sbjct: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of CmaCh01G014110 vs. NCBI nr
Match: XP_022941000.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 572/579 (98.79%), Postives = 576/579 (99.48%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPDDHSDDE+FSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLE RRASVRAWGNQS
Sbjct: 61  DPDDHSDDEQFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEVRRASVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKI+ICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKIVICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHG+ETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
            SLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 TSLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKLHKD+MKNLQNNKDIMELRNRVEAFAASFAMPGFDI
Sbjct: 541 HGKLHKDMMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of CmaCh01G014110 vs. NCBI nr
Match: XP_023524836.1 (serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 572/579 (98.79%), Postives = 573/579 (98.96%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60
           MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120
           DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARR SVRAWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRTSVRAWGNQS 120

Query: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240
           NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360
           YPREWDYGRCRQIADKCG ILMSDMSHISGLVAAKEC SPFQYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGGILMSDMSHISGLVAAKECASPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGVLLNHG+ETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480
           ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHRE 540

Query: 541 HGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           HGKL KDIMKNLQNNKDIMELRNRVEAFAAS AMPGFDI
Sbjct: 541 HGKL-KDIMKNLQNNKDIMELRNRVEAFAASLAMPGFDI 578

BLAST of CmaCh01G014110 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 804.7 bits (2077), Expect = 4.9e-233
Identity = 403/592 (68.07%), Postives = 471/592 (79.56%), Query Frame = 0

Query: 1   MDFRHAQSDLSLRFS-SHAT---SSISRSSIGDNS-------SYLCSTASNSSYLLELRE 60
           MD   +Q++  L F  SHA+   +   R+ I D+S       S+     + S   L+L E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  QKDEKFNFVK------GDPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDE 120
           QK EK   V       G   D  +DE F +LG  + +KRQRD    L Q    + + + +
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGD 120

Query: 121 SSLEARRASVRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSH 180
           S LE+RRA+VRAWG+Q + +ADP+IH++M KEKQRQ RGIEL+ASENFVCRAVMEALGSH
Sbjct: 121 SDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSH 180

Query: 181 LTNKYSEGMPGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVY 240
           LTNKYSEGMPGARYYTGNQ+ID+IE LC ERAL AF L+ +KWGVNVQPYSCTSANFAVY
Sbjct: 181 LTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVY 240

Query: 241 TGLLHPKDRIMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLE 300
           TGLL P +RIMGLDS SGGH+SHGY TPGGKKISAASIFF+S PYKVNP TGY+DYDKLE
Sbjct: 241 TGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLE 300

Query: 301 EKALDYRPKILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQY 360
           +KALDYRPKILICGGSSYPR+WD+ R RQIADKCGA+LM DM+HISGLVA KEC +PF +
Sbjct: 301 DKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDH 360

Query: 361 CDIVTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQG 420
           CDIVTSTTHK LRGPRGGIIF+RRG K RKQG   +H D +  YD E++INF+VFPSLQG
Sbjct: 361 CDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQG 420

Query: 421 GPHNNHIAALAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWD 480
           GPHNNHIAALAIALKQVA+ EY+ YI+Q+KKNAQALA+ALLRRKCRLVT GTDNHL+LWD
Sbjct: 421 GPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWD 480

Query: 481 LIPLGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIA 540
           L P+GLT K YEK+CE+CHIT+NK AI+GDNG ISPGGVRIGTPAMTTRGC+E+DFET+A
Sbjct: 481 LTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMA 540

Query: 541 EILLKAVQITKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMP 576
           + L+KA QIT   L REHGK HK+ +K+L  NKDI ELRNRVEAFA  + MP
Sbjct: 541 DFLIKAAQITS-ALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of CmaCh01G014110 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 787.7 bits (2033), Expect = 6.3e-228
Identity = 400/598 (66.89%), Postives = 474/598 (79.26%), Query Frame = 0

Query: 4   RHAQSDLSLRF-SSHA--TSSISRSSIGD-------NSSYLCSTASNSSYLLELREQK-- 63
           R AQSDLSL F SSHA       R  I D       +SS+  S+       L+L EQ+  
Sbjct: 3   RIAQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFD 62

Query: 64  --------DEKFNFVKG--DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIM 123
                    E+ + V G  D DD  ++E+F LLG P+++KR R   S+    S+     +
Sbjct: 63  VTGSCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFV 122

Query: 124 DESSLEARRASVRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALG 183
            +S +E+RRA VRAWGNQS+  ADPEIH+ M KEKQRQ+RGIEL+ASENFVCRAVMEALG
Sbjct: 123 VDSGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALG 182

Query: 184 SHLTNKYSEGMPGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFA 243
           SHLTNKYSEGMPGARYYTGNQ+ID+IE+LC ERALAAF L+ EKWGVNVQPYSCTSANFA
Sbjct: 183 SHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFA 242

Query: 244 VYTGLLHPKDRIMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDK 303
           V+TGLL P +RIMGLDS SGGH+SHGYYTPGGKK+S ASIFF+S PYKV+P TGY+DYDK
Sbjct: 243 VFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDK 302

Query: 304 LEEKALDYRPKILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPF 363
           LEEKALDYRPKILICGGSSYPR+W++ R R IADKCGA+LM DM+ ISGLVAAKE  +PF
Sbjct: 303 LEEKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPF 362

Query: 364 QYCDIVTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSL 423
            YCDIVTSTTHKSLRGPRGGIIF++RGLK +KQ + LNH +    YDFE++INFSVFPSL
Sbjct: 363 DYCDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSL 422

Query: 424 QGGPHNNHIAALAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVL 483
           QGGPHNNHIAALAIALKQ AS EY++Y+RQVKKNA+ALASAL+ RKC+L+T GTDNHL+L
Sbjct: 423 QGGPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLL 482

Query: 484 WDLIPLGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFET 543
           WDL PLGLT K YEK+CE+CHITVNK AI+ +NG ISPGGVRIG+PAMT+RGCLE +FET
Sbjct: 483 WDLTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFET 542

Query: 544 IAEILLKAVQITKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           +A+ L +A QI      REHGKL K+ +K++ + K+I +LRN+VEAFA  FAMP FD+
Sbjct: 543 MADFLYRAAQIAS-AAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of CmaCh01G014110 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 624.4 bits (1609), Expect = 9.2e-179
Identity = 300/465 (64.52%), Postives = 366/465 (78.71%), Query Frame = 0

Query: 113 VRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN SL   DPEIHD++ KEK+RQ RGIEL+ASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 173 PGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDR 232
           PG RYY GN+ IDEIE LC  RAL AF  DP  WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGHL+HGYYT GGKKISA SI+F+SLPYKVN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 293 ILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTH 352
           +LICGGS+YPR+WDY R R IADK GA+L+ DM+HISGLVAA+E  +PF+YCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAA 412
           KSLRGPR G+IF+R+G K  K+G       E   YDFED+INF+VFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 413 LAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAK 472
           LA+ALKQ  +  ++VY +QVK NA AL + L+ +  ++VT+GT+NHLVLWDL PLGLT  
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 473 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQI 532
             EK+C+LC IT+NK A++GD+ A++PGGVRIG PAMT+RG +E DFE I E L +AV +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 533 TKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGF 578
           T + + + +GKL KD  K L NNKD+ +L+  VE F+AS+ MPGF
Sbjct: 424 T-LDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of CmaCh01G014110 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 603.2 bits (1554), Expect = 2.2e-172
Identity = 289/467 (61.88%), Postives = 355/467 (76.02%), Query Frame = 0

Query: 113 VRAWGNQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  L   DPEI+D++ KEK RQ RGIEL+A+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 173 PGARYYTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDR 232
           PG RYY G + IDEIE LC  R+L AF  +PEKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGH++HGYY+ GGK ISA SI+F++LPYKV+  TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 293 ILICGGSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTH 352
           ++ICGG+SYPREWDY R R +ADK GA L+ DM+H S LVAA+E   PF+YCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAA 412
           KSLRGPR G+IF+R+G K  K+G       E   YDF+ +IN +VFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 413 LAIALKQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAK 472
           LA+ALKQV +  ++VY +QVK NA  LAS L+ +   LVTDGTDNHL+LWDL PLGLT  
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 473 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQI 532
             EK+CELC+IT+N+ A++GD   ++PGGVRIGTPAMT+RG +E DFE I E L +AV I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 533 TKMVLHREHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 580
           T + +  ++GK+ KD  K L NNK+I E++  VE F   F MPGF I
Sbjct: 424 T-LDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of CmaCh01G014110 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 482.6 bits (1241), Expect = 4.3e-136
Identity = 242/465 (52.04%), Postives = 316/465 (67.96%), Query Frame = 0

Query: 118 NQSLRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARY 177
           N  L   DPEI DI+  EK RQ++G+EL+ SENF   +VM+A+GS +TNKYSEG PGARY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 178 YTGNQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLD 237
           Y GN++ID  E LC +RAL AF LDPEKWGVNVQP S + ANF VYT LL P +RIM LD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 238 SASGGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICG 297
              GGHLSHGY T   KKISA SIFF+++PY+++  TGY+DYD++E+ A  +RPK+++ G
Sbjct: 172 LPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 298 GSSYPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRG 357
            S+Y R +DY R R++ +K  A++++DM+HISGLVAA    SPF Y D+VT+TTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 358 PRGGIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIAL 417
           PRG +IFFR+G+K       +N   +   YDFED+IN +VFP LQGGPHN+ I  LA+AL
Sbjct: 292 PRGAMIFFRKGVKE------INKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVAL 351

Query: 418 KQVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKI 477
           KQ  + EY+ Y  QV  N+   A  L+ R   LV+ GTDNHLVL +L P G+     EK+
Sbjct: 352 KQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKV 411

Query: 478 CELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVL 537
            E  HI  NK  + GD  A+ PGG+R+GTPA+T+RG +E DF  +AE   KAV I   V 
Sbjct: 412 LEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVK 471

Query: 538 HREHGKLHKDIMKNLQNN----KDIMELRNRVEAFAASFAMPGFD 579
               G   KD +  ++++     +I +LR+ VE FA  F   GF+
Sbjct: 472 SEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WV07.0e-23268.07Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM598.8e-22766.89Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
O232541.3e-17764.52Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM43.1e-17161.88Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q54EW11.4e-15255.77Serine hydroxymethyltransferase 2 OS=Dictyostelium discoideum OX=44689 GN=shmt2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IY330.0e+00100.00Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480261 PE=3... [more]
A0A6J1FSB10.0e+0098.79Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446415 PE... [more]
A0A6J1C5987.3e-30991.24Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111008604 P... [more]
A0A5A7VB491.2e-29887.97Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B9K51.2e-29887.97Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103487552 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_022980970.10.0e+00100.00serine hydroxymethyltransferase 7-like [Cucurbita maxima][more]
KAG7031702.10.0e+0099.31Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6608069.10.0e+0099.14Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941000.10.0e+0098.79serine hydroxymethyltransferase 7-like [Cucurbita moschata][more]
XP_023524836.10.0e+0098.79serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G36370.14.9e-23368.07serine hydroxymethyltransferase 7 [more]
AT1G22020.16.3e-22866.89serine hydroxymethyltransferase 6 [more]
AT4G13930.19.2e-17964.52serine hydroxymethyltransferase 4 [more]
AT4G13890.12.2e-17261.88Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G37930.14.3e-13652.04serine transhydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 123..525
e-value: 3.2E-170
score: 566.0
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 150..430
e-value: 3.1E-194
score: 647.4
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 125..575
e-value: 3.1E-194
score: 647.4
NoneNo IPR availablePIRSRPIRSR000412-50PIRSR000412-50coord: 120..577
e-value: 1.7E-166
score: 551.9
NoneNo IPR availablePANTHERPTHR11680:SF7SERINE HYDROXYMETHYLTRANSFERASE 7coord: 78..579
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 78..579
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 120..576
score: 38.427467
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 125..568
e-value: 0.0
score: 554.119
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 345..361
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 111..578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G014110.1CmaCh01G014110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity