CmaCh01G012970 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G012970
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCholine-phosphate cytidylyltransferase
LocationCma_Chr01: 9293206 .. 9297633 (-)
RNA-Seq ExpressionCmaCh01G012970
SyntenyCmaCh01G012970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAATAAAAGAAACAAATAAATAGGGAGCGGAAACATCGCGTTGTCGGAGGAGAAGAAATCCACAACAAGAGGGCTCGCCGAAGAAGCAGAACGGAAGCGAAAGGGAAGAGAAGAGGTCGGAAGATAATTCGAATGGCAGAGGTGAAGCCCGCCGATTCCACCACCACTATCAACGCTTCTCCTAACCATTCCAATCCTGATTACCCGACGGACCGCCCAGTTCGAGTTTACGCCGATGGGATCTACGATCTCTTTCATTTCGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCGTCCGTTTCTTCTCCTTTTCCTTAATTTCTTTTCGTTTTTTTTTTGTTATTCCTCATATGCTGCATCTGTGACTGTAGTTTCCATGATTTTTTTATGGTTTCTGATACTTTTCGATCTAAGTAATTTAGTTCCTGTGTTTCGCCGGGTGGTAGGAATTGTCGTTTTCTCTTATTGTGTGTTTTTTTTTTCTTTTTCTGATTCATTAGCCTGCTTGATTGTTCTTAATTTTCAGGTTTGCTAACTTGCTTTCACTTGTCCGTGATCTTCTTCTGTTGGGAGAATTGATTTGTTGAATTGACGAGCTGTAATTATTGATCCTTCTTGTAATTTCAGATTCCCGAATACTTATTTGTTAGTTGGATGTTGCAATGATGAGACTACCCACAATCTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTATGACTTTTCTCTACTCCATGAATTTTCTTTTTACTGGGGCTTTTGGATCTTGAAACATTGTTTGTATAGATGTGCTTTTGATACCTACAAACGCTAGTTAGAATGCCTGTTTGAATCCTTTAAATTCAAGTGTAGCTATGATGGATTTAAATTGAACTCTCAATTTTTCGATATCTATTTGAGCTTTCTTTATAGATTTTCTTATGAATGTGGAATCAATCTTAGTTGCACTGATTCTTCAACGTGGTTTTATTTGTTTGCTCCTTGTTTTCCCCTCCCTTTTCCGCAGATGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCACGATTCTCTTCCGTAAGGATATTATATACTTTATCTAAGTGAACCAAGAGGGTCAAGTAACTTTTGGGCAATGGATTTTATTTCCTTTACCACATTTATCTGCATTTCTACCCATCTAATTAGTATAGATTGGATGGTAAAGTGTCTCAGGAAGAGCTCCAATTAGTTGGTCCATGGTATCTATCTGTTCATATTGAATTTATTTGTTAATTCTGTTTTAAATGACCTATGTCATCCTTTCTCTGCCTCGATACAGTTATGCTGATGCTACCGGAGCTGGGAAGGATGTTTATGAATTTGTAAGATCAGAAGATGGGTTTATCCATTCGATCCACTTGGTGCTTAAGTACATACAATTTCATAGATAGGAACTTTTCCTTTGTAGGTTAAAAAAATTGGAAAGTTCAAGGAAACAAAAAGGACGGAAGGAATATCAACTTCAGACATTATCATGAGGATTGTCAAAGATTATAATCAATATGTTCTGCGTAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGTGAAGGCATGATATTTTATCTACATTGACCCTTTTTATAACGATAATTCTTATCCTGCTAGTCAGCAAAGCATGATTATATTGATTTTGTTTATTAACAAGCAACTCAATCTTGTTGTACTCAATAACCAGGAAAAGCGGTTAAGAGTGAATATGAGATTGAAGAAATTACAGGAGAAAGTGAAGGAACATCAAGAAAAAGTGGGAGAAAAGGTACCCATATCTCATATCTTTACAATAGTCCTATGGATGTGGTAGTTGGGGAGTTGAGATTAGATTATCTGCTTTATAATTCCTCTTATTTCAGGCTATGGGATAGGTGTACCTTAGGTGTTGCATTAAAAAATTTCTATATGCACAAAAGCTGGTCCTAACACTTACTAATATAACAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAGAGGAAGCTGGGCAGTCCACTTGCAATCTTGCAATTTTGTTACTAGAATTTGCCTAAAGGCGTGGCTTATGTTTTGTGCATTTTTGCTATGACTTGATAGCTAACCATAAGTTTCAGGAAAAAAAGCTAGAGATATTCACATTAGAGAAAGCCAATATTATTGGATGAAATCCATATTTCTACGGTTTTATTTTCAATAAACTTAGCTATGCACTGTGTGCTTTTGGGCCCACCTTTTTCACCTTGCCTGGTGTGTGCATCTTAAGATGCCTCGATGTTTTTACGACTCATAATTATTTCGAATGATCAGATACTTCACTGTCCTTGCACATAAGCGAATATGATTTCTTGACCAATTGAAGAACTTAGATGAGATGATTCTATGCTACATTTTGTATCTTCCTGAAATGACTTGAATTAAGACACCAAAAGAGCAAAACACGAAAGAACAACTTGTTGAAATGTACCTATGTGTTTTTTCTGCCTTCAGTTGATTTTAGAACTTACTCCAAAAATAATGTGGTCTGTGCGGCTGCCTTTCACATTTCTTTTTGTTTTATTTCATTTTGTGATATATCTTCTTAGAAAAGGATTCTAATTGTACATTCATGAAGTTGTCTTTTCAAGTAAGAAAAGTTGACTTTTGAGCCAGCTATCTACCATGCAAGCAGTATAATTCTTTTGGGTTCTTTTTTTGAGCACGTGAAACGATTCCAGAAACAGCTGTGCTGATATCAAAGTATGGAACCCTAGTATTTCAATGTATTCACAGACATATTCTTGCTTTTCAATTCTTTCTTATGCTCGTAAATCCCAACTTTTGTTTCTGTACTAATTCAAGCATTGAAGTTTTTATTGGTAATTGATATTTAGGCCGCCTAATATAGCAAGTTGATTGCTTCTTGGGTGTATTCTATTCTCCCCATTGAAGTGAAAAAAGTCCTTTTTTGTACTTCAGTGCTAGCGAGGACTTTATCTGGTTTTGTTAGACCTACAATTATATTTTGGGCTTTATTTTCCTCGATCTAATTATTGAGGCAGGATATTTAATTATTGTTTTTTGTTGAAAATGATTGTTCTGGATACTGCAGATTCAAACAGTGGCAAAAACTGCCGGCATGCACAGGAATGAGTGGGTGGAAAATGCTGATCGCTGGGTGGCTGGATTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGTAAGTTAATGCACGAAGTATCCTGTTCTTCATTTACGTGGCAAGTTTTGAAGTAGATAAGAAAAAGAAAGCATACAAAAACATACATGGTTTTGTGATTCTTGAACTAAAACAAAGGGTTAAATCTGAAACATAACGGGTGTATTTAGGGCACGGCCATCAGAGACCGAATTCAAGAGCGGTTAATGGGGGCACAGCAGCAAAAATATCTAGAAAATGGCAAGGACAGTGACTCAGACCTCGAAGAAGAATATTATTACGATGACGACGACGAAGAAGAATATTACGAAGACGGCTTCTACGAAGAATTCGAGAAAGACGAGAAGAAAAGGTAGACACGTATTTACATCAAGTTCGAATACATGTCACATGCATAATTTGATGCTTGTTCATGGATTCTACAGTAGTATGTTTCTGCTATATGTTTAGGCGCAGACAGTAAACGTAAACCATAACTGCTAATTGCCAAAGCAAAAAAAGAAAACAAAAGAGGGGCACTTGGCGGTGAGTTCAGGAACCAATGTGGCTTTTGTGACCCTTTTTAAGATATGTGTTTGTGCATATAGGTTGAAATTTTAACCCTTGAAGCTTGTAAAACAGGTCGTTTCTTTTTGGGTTTTGTTTGTGAATCATTGTAATTGAAGAGTTTTGAAGCTTGGGGTTTCCTGTTTGTATGTATTGTAAGAGTGATAAATATGGTTTGATATTATTATATGTATCTTTCTCTTTGTTTTTGGTCATTCTGTAAAGGAGAATTGAAAGGGAATTTGTGAAAAGCATTAGATTAGTGGGATACTTGAAAGGAG

mRNA sequence

GAGAAAATAAAAGAAACAAATAAATAGGGAGCGGAAACATCGCGTTGTCGGAGGAGAAGAAATCCACAACAAGAGGGCTCGCCGAAGAAGCAGAACGGAAGCGAAAGGGAAGAGAAGAGGTCGGAAGATAATTCGAATGGCAGAGGTGAAGCCCGCCGATTCCACCACCACTATCAACGCTTCTCCTAACCATTCCAATCCTGATTACCCGACGGACCGCCCAGTTCGAGTTTACGCCGATGGGATCTACGATCTCTTTCATTTCGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCATTCCCGAATACTTATTTGTTAGTTGGATGTTGCAATGATGAGACTACCCACAATCTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAATGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCACGATTCTCTTCCTTATGCTGATGCTACCGGAGCTGGGAAGGATGTTTATGAATTTGTTAAAAAAATTGGAAAGTTCAAGGAAACAAAAAGGACGGAAGGAATATCAACTTCAGACATTATCATGAGGATTGTCAAAGATTATAATCAATATGTTCTGCGTAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGAAAAGCGGTTAAGAGTGAATATGAGATTGAAGAAATTACAGGAGAAAGTGAAGGAACATCAAGAAAAAGTGGGAGAAAAGATTCAAACAGTGGCAAAAACTGCCGGCATGCACAGGAATGAGTGGGTGGAAAATGCTGATCGCTGGGTGGCTGGATTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGGCACGGCCATCAGAGACCGAATTCAAGAGCGGTTAATGGGGGCACAGCAGCAAAAATATCTAGAAAATGGCAAGGACAGTGACTCAGACCTCGAAGAAGAATATTATTACGATGACGACGACGAAGAAGAATATTACGAAGACGGCTTCTACGAAGAATTCGAGAAAGACGAGAAGAAAAGGTAGACACGTATTTACATCAAGTTCGAATACATGTCACATGCATAATTTGATGCTTGTTCATGGATTCTACAGTAGTATGTTTCTGCTATATGTTTAGGCGCAGACAGTAAACGTAAACCATAACTGCTAATTGCCAAAGCAAAAAAAGAAAACAAAAGAGGGGCACTTGGCGGTGAGTTCAGGAACCAATGTGGCTTTTGTGACCCTTTTTAAGATATGTGTTTGTGCATATAGGTTGAAATTTTAACCCTTGAAGCTTGTAAAACAGGTCGTTTCTTTTTGGGTTTTGTTTGTGAATCATTGTAATTGAAGAGTTTTGAAGCTTGGGGTTTCCTGTTTGTATGTATTGTAAGAGTGATAAATATGGTTTGATATTATTATATGTATCTTTCTCTTTGTTTTTGGTCATTCTGTAAAGGAGAATTGAAAGGGAATTTGTGAAAAGCATTAGATTAGTGGGATACTTGAAAGGAG

Coding sequence (CDS)

ATGGCAGAGGTGAAGCCCGCCGATTCCACCACCACTATCAACGCTTCTCCTAACCATTCCAATCCTGATTACCCGACGGACCGCCCAGTTCGAGTTTACGCCGATGGGATCTACGATCTCTTTCATTTCGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCATTCCCGAATACTTATTTGTTAGTTGGATGTTGCAATGATGAGACTACCCACAATCTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAATGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCACGATTCTCTTCCTTATGCTGATGCTACCGGAGCTGGGAAGGATGTTTATGAATTTGTTAAAAAAATTGGAAAGTTCAAGGAAACAAAAAGGACGGAAGGAATATCAACTTCAGACATTATCATGAGGATTGTCAAAGATTATAATCAATATGTTCTGCGTAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGAAAAGCGGTTAAGAGTGAATATGAGATTGAAGAAATTACAGGAGAAAGTGAAGGAACATCAAGAAAAAGTGGGAGAAAAGATTCAAACAGTGGCAAAAACTGCCGGCATGCACAGGAATGAGTGGGTGGAAAATGCTGATCGCTGGGTGGCTGGATTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGGCACGGCCATCAGAGACCGAATTCAAGAGCGGTTAATGGGGGCACAGCAGCAAAAATATCTAGAAAATGGCAAGGACAGTGACTCAGACCTCGAAGAAGAATATTATTACGATGACGACGACGAAGAAGAATATTACGAAGACGGCTTCTACGAAGAATTCGAGAAAGACGAGAAGAAAAGGTAG

Protein sequence

MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEYYEDGFYEEFEKDEKKR
Homology
BLAST of CmaCh01G012970 vs. ExPASy Swiss-Prot
Match: Q9ZV56 (Choline-phosphate cytidylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CCT1 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.9e-122
Identity = 231/318 (72.64%), Postives = 268/318 (84.28%), Query Frame = 0

Query: 7   ADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP-NTYLLVGC 66
           A  +T + +SP       PTDRPVRVYADGIYDLFHFGHARSLEQAK +FP NTYLLVGC
Sbjct: 19  ASGSTAVQSSP-------PTDRPVRVYADGIYDLFHFGHARSLEQAKLAFPNNTYLLVGC 78

Query: 67  CNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLP 126
           CNDETTH  KG+TVM   ERYESLRHCKWVDEVIPDAPWV++QEF+DKH+ID+VAHDSLP
Sbjct: 79  CNDETTHKYKGRTVMTAEERYESLRHCKWVDEVIPDAPWVVNQEFLDKHQIDYVAHDSLP 138

Query: 127 YADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKEL 186
           YAD++GAGKDVYEFVKK+G+FKET+RTEGISTSDIIMRIVKDYNQYV+RNLDRGYSR++L
Sbjct: 139 YADSSGAGKDVYEFVKKVGRFKETQRTEGISTSDIIMRIVKDYNQYVMRNLDRGYSREDL 198

Query: 187 GVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFL 246
           GVSF  EKRLRVNMRLKKLQE+VKE QE+VGEKIQTV     M RNEWVENADRWVAGFL
Sbjct: 199 GVSFVKEKRLRVNMRLKKLQERVKEQQERVGEKIQTVK----MLRNEWVENADRWVAGFL 258

Query: 247 EMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSD--LEEEYY-----YDDDDEE 306
           E+FEEGCHKMGTAI D IQERLM  +  + LENG+D D+D    EEY+      DDD++E
Sbjct: 259 EIFEEGCHKMGTAIVDSIQERLMRQKSAERLENGQDDDTDDQFYEEYFDHDMGSDDDEDE 318

Query: 307 EYYEDGFYEEFEKDEKKR 315
           ++Y++   EE +++E ++
Sbjct: 319 KFYDE---EEVKEEETEK 322

BLAST of CmaCh01G012970 vs. ExPASy Swiss-Prot
Match: F4JJE0 (Choline-phosphate cytidylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CCT2 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.0e-120
Identity = 218/276 (78.99%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 26  TDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAER 85
           +DRPVRVYADGI+DLFHFGHAR++EQAKKSFPNTYLLVGCCNDE T+  KGKTVM E+ER
Sbjct: 17  SDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITNKFKGKTVMTESER 76

Query: 86  YESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGK 145
           YESLRHCKWVDEVIPDAPWV++ EF+DKHKID+VAHD+LPYAD +GAG DVYEFVK IGK
Sbjct: 77  YESLRHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGAGNDVYEFVKSIGK 136

Query: 146 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF-EKRLRVNMRLKKLQE 205
           FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSR+ELGVSF EKRLRVNMRLKKLQE
Sbjct: 137 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSREELGVSFEEKRLRVNMRLKKLQE 196

Query: 206 KVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQER 265
           KVKE Q    EKIQTVAKTAGMH +EW+ENADRWVAGFLEMFEEGCHKMGTAIRD IQ+R
Sbjct: 197 KVKEQQ----EKIQTVAKTAGMHHDEWLENADRWVAGFLEMFEEGCHKMGTAIRDGIQQR 256

Query: 266 LM---GAQQQKYLENGKDSDSDLEEEYYYDDDDEEE 298
           LM     + ++ L+NG     D ++E   DD++  E
Sbjct: 257 LMRQESEENRRLLQNGLTISKDNDDEQMSDDNEFAE 288

BLAST of CmaCh01G012970 vs. ExPASy Swiss-Prot
Match: Q9Y5K3 (Choline-phosphate cytidylyltransferase B OS=Homo sapiens OX=9606 GN=PCYT1B PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 3.1e-69
Identity = 130/224 (58.04%), Postives = 165/224 (73.66%), Query Frame = 0

Query: 25  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAE 84
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN+YLLVG C+D+ TH  KG TVMNEAE
Sbjct: 72  PADRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 85  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIG 144
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY+ A     DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSA--GSDDVYKHIKEAG 191

Query: 145 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF--EKRLRVNMRLKKL 204
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF  EKR R   ++ K+
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKRYRFQNQVDKM 251

Query: 205 QEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 247
           +EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 KEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CmaCh01G012970 vs. ExPASy Swiss-Prot
Match: Q811Q9 (Choline-phosphate cytidylyltransferase B OS=Mus musculus OX=10090 GN=Pcyt1b PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 7.0e-69
Identity = 129/224 (57.59%), Postives = 165/224 (73.66%), Query Frame = 0

Query: 25  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAE 84
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN+YLLVG C+D+ TH  KG TVMNEAE
Sbjct: 72  PVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 85  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIG 144
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY+ A     DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSA--GSDDVYKHIKEAG 191

Query: 145 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF--EKRLRVNMRLKKL 204
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF  EK+ R   ++ K+
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKM 251

Query: 205 QEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 247
           +EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 KEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CmaCh01G012970 vs. ExPASy Swiss-Prot
Match: Q9QZC4 (Choline-phosphate cytidylyltransferase B OS=Rattus norvegicus OX=10116 GN=Pcyt1b PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.0e-69
Identity = 129/224 (57.59%), Postives = 165/224 (73.66%), Query Frame = 0

Query: 25  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAE 84
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN+YLLVG C+D+ TH  KG TVMNEAE
Sbjct: 72  PVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 85  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIG 144
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY+ A     DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSA--GSDDVYKHIKEAG 191

Query: 145 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF--EKRLRVNMRLKKL 204
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF  EK+ R   ++ K+
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKM 251

Query: 205 QEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 247
           +EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 KEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CmaCh01G012970 vs. ExPASy TrEMBL
Match: A0A6J1J168 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480356 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.2e-176
Identity = 314/316 (99.37%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240
           SRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW
Sbjct: 181 SRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240

Query: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300
           VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY
Sbjct: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300

Query: 301 YEDGFYEEFEKDEKKR 315
           YEDGFYEEFEKDEKKR
Sbjct: 301 YEDGFYEEFEKDEKKR 316

BLAST of CmaCh01G012970 vs. ExPASy TrEMBL
Match: A0A6J1FJ47 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445839 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.0e-173
Identity = 311/316 (98.42%), Postives = 312/316 (98.73%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTH+LKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240
           SRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW
Sbjct: 181 SRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240

Query: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300
           VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDD EEY
Sbjct: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDD-EEY 300

Query: 301 YEDGFYEEFEKDEKKR 315
           YED FYEEFEKDEKKR
Sbjct: 301 YEDDFYEEFEKDEKKR 315

BLAST of CmaCh01G012970 vs. ExPASy TrEMBL
Match: A0A1S3CM33 (choline-phosphate cytidylyltransferase 2-like OS=Cucumis melo OX=3656 GN=LOC103502481 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.2e-161
Identity = 295/328 (89.94%), Postives = 301/328 (91.77%), Query Frame = 0

Query: 1   MAEVKPADSTTTI---------NASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQ 60
           MAEVKP DSTT I         N S NHSN D PTDRPVRVYADGIYDLFHFGHARSLEQ
Sbjct: 1   MAEVKPGDSTTNITTDTTTTAFNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHARSLEQ 60

Query: 61  AKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFI 120
           AKKSFPNT+LLVGCCNDETTH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQEFI
Sbjct: 61  AKKSFPNTFLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQEFI 120

Query: 121 DKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY 180
           DKHKIDFVAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY
Sbjct: 121 DKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY 180

Query: 181 VLRNLDRGYSRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRN 240
           VLRNLDRGYSRKELGVS+  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRN
Sbjct: 181 VLRNLDRGYSRKELGVSYVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRN 240

Query: 241 EWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYY 300
           EWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQ KY+ NGKDSDSDL+EEYY
Sbjct: 241 EWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQLKYISNGKDSDSDLDEEYY 300

Query: 301 YDDDDEEEYY---EDGFYEEFEKDEKKR 315
           YDDDD EEYY   +D FYEEFEKDEKKR
Sbjct: 301 YDDDDNEEYYDDDDDDFYEEFEKDEKKR 328

BLAST of CmaCh01G012970 vs. ExPASy TrEMBL
Match: A0A6J1FCK6 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442868 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.6e-159
Identity = 294/332 (88.55%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 1   MAEVKPADS------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARS 60
           MAEV P +S            TTTIN S NHSN D PTDRPVRVYADGIYDLFHFGHARS
Sbjct: 1   MAEVNPGESTTTTTTITTTTTTTTINGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHARS 60

Query: 61  LEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQ 120
           LEQAKKSFPNTYLLVGCCNDETTH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQ
Sbjct: 61  LEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQ 120

Query: 121 EFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDY 180
           EFIDKH ID+VAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVKDY
Sbjct: 121 EFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVKDY 180

Query: 181 NQYVLRNLDRGYSRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGM 240
           NQYVLRNLDRGYSRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGM
Sbjct: 181 NQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGM 240

Query: 241 HRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSD-SDLE 300
           HRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYL NGKDSD SDL+
Sbjct: 241 HRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDYSDLD 300

Query: 301 EEYYYDDDDEEEYY---EDGFYEEFEKDEKKR 315
           EEYYYDD+D EEYY   +D FYEEFEKDEKKR
Sbjct: 301 EEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CmaCh01G012970 vs. ExPASy TrEMBL
Match: A0A6J1IPQ3 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477078 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.9e-159
Identity = 293/332 (88.25%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 1   MAEVKPADS------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARS 60
           MAEV P +S            TTT+N S NHSN D PTDRPVRVYADGIYDLFHFGHARS
Sbjct: 1   MAEVNPGESTTTTTTITTTTTTTTVNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHARS 60

Query: 61  LEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQ 120
           LEQAKKSFPNTYLLVGCCNDETTH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQ
Sbjct: 61  LEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQ 120

Query: 121 EFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDY 180
           EFIDKH ID+VAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVKDY
Sbjct: 121 EFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVKDY 180

Query: 181 NQYVLRNLDRGYSRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGM 240
           NQYVLRNLDRGYSRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGM
Sbjct: 181 NQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGM 240

Query: 241 HRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSD-SDLE 300
           HRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYL NGKDSD SDL+
Sbjct: 241 HRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDYSDLD 300

Query: 301 EEYYYDDDDEEEYY---EDGFYEEFEKDEKKR 315
           EEYYYDD+D EEYY   +D FYEEFEKDEKKR
Sbjct: 301 EEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CmaCh01G012970 vs. NCBI nr
Match: XP_022981108.1 (choline-phosphate cytidylyltransferase 2-like [Cucurbita maxima])

HSP 1 Score: 626.7 bits (1615), Expect = 1.1e-175
Identity = 314/316 (99.37%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240
           SRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW
Sbjct: 181 SRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240

Query: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300
           VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY
Sbjct: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300

Query: 301 YEDGFYEEFEKDEKKR 315
           YEDGFYEEFEKDEKKR
Sbjct: 301 YEDGFYEEFEKDEKKR 316

BLAST of CmaCh01G012970 vs. NCBI nr
Match: XP_022940119.1 (choline-phosphate cytidylyltransferase 2-like [Cucurbita moschata])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 311/316 (98.42%), Postives = 312/316 (98.73%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTH+LKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240
           SRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW
Sbjct: 181 SRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240

Query: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300
           VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDD EEY
Sbjct: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDD-EEY 300

Query: 301 YEDGFYEEFEKDEKKR 315
           YED FYEEFEKDEKKR
Sbjct: 301 YEDDFYEEFEKDEKKR 315

BLAST of CmaCh01G012970 vs. NCBI nr
Match: KAG6607965.1 (Choline-phosphate cytidylyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615.9 bits (1587), Expect = 1.9e-172
Identity = 309/315 (98.10%), Postives = 311/315 (98.73%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVK ADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKSADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTH+LKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240
           SRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW
Sbjct: 181 SRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240

Query: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300
           VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDD+EEY
Sbjct: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDDEEY 300

Query: 301 YEDGFYEEFEKDEKK 314
           YED FYEEFEKDEKK
Sbjct: 301 YEDDFYEEFEKDEKK 315

BLAST of CmaCh01G012970 vs. NCBI nr
Match: XP_023523876.1 (choline-phosphate cytidylyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.8 bits (1584), Expect = 4.2e-172
Identity = 308/316 (97.47%), Postives = 312/316 (98.73%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVK ADSTTT+NASPN+SNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKSADSTTTLNASPNYSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTH+LKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240
           SRKELGVSF  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW
Sbjct: 181 SRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRW 240

Query: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEEY 300
           VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDD+EEY
Sbjct: 241 VAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDDEEY 300

Query: 301 YEDGFYEEFEKDEKKR 315
           YED FYEEFEKDEKKR
Sbjct: 301 YEDDFYEEFEKDEKKR 316

BLAST of CmaCh01G012970 vs. NCBI nr
Match: XP_038898408.1 (choline-phosphate cytidylyltransferase 2-like [Benincasa hispida])

HSP 1 Score: 578.2 bits (1489), Expect = 4.4e-161
Identity = 295/321 (91.90%), Postives = 300/321 (93.46%), Query Frame = 0

Query: 1   MAEVKPADSTTT---INASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP 60
           MAEVKP DSTTT   +N S N SN D PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP
Sbjct: 1   MAEVKPGDSTTTTTALNGSQNRSNSDQPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP 60

Query: 61  NTYLLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKID 120
           NTYLLVGCCNDE TH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQEFIDKH ID
Sbjct: 61  NTYLLVGCCNDEITHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHNID 120

Query: 121 FVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLD 180
           FVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLD
Sbjct: 121 FVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLD 180

Query: 181 RGYSRKELGVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENA 240
           RGYSRKELGVS+  EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENA
Sbjct: 181 RGYSRKELGVSYVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENA 240

Query: 241 DRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDE 300
           DRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYL NGKDSDSDL+EEYYYDD+D 
Sbjct: 241 DRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDSDLDEEYYYDDEDN 300

Query: 301 EEYY--EDGFYEEFEKDEKKR 315
           EEYY  +D FYEEFEKDEKKR
Sbjct: 301 EEYYDDDDDFYEEFEKDEKKR 321

BLAST of CmaCh01G012970 vs. TAIR 10
Match: AT2G32260.1 (phosphorylcholine cytidylyltransferase )

HSP 1 Score: 440.3 bits (1131), Expect = 1.3e-123
Identity = 231/318 (72.64%), Postives = 268/318 (84.28%), Query Frame = 0

Query: 7   ADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP-NTYLLVGC 66
           A  +T + +SP       PTDRPVRVYADGIYDLFHFGHARSLEQAK +FP NTYLLVGC
Sbjct: 19  ASGSTAVQSSP-------PTDRPVRVYADGIYDLFHFGHARSLEQAKLAFPNNTYLLVGC 78

Query: 67  CNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLP 126
           CNDETTH  KG+TVM   ERYESLRHCKWVDEVIPDAPWV++QEF+DKH+ID+VAHDSLP
Sbjct: 79  CNDETTHKYKGRTVMTAEERYESLRHCKWVDEVIPDAPWVVNQEFLDKHQIDYVAHDSLP 138

Query: 127 YADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKEL 186
           YAD++GAGKDVYEFVKK+G+FKET+RTEGISTSDIIMRIVKDYNQYV+RNLDRGYSR++L
Sbjct: 139 YADSSGAGKDVYEFVKKVGRFKETQRTEGISTSDIIMRIVKDYNQYVMRNLDRGYSREDL 198

Query: 187 GVSF--EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFL 246
           GVSF  EKRLRVNMRLKKLQE+VKE QE+VGEKIQTV     M RNEWVENADRWVAGFL
Sbjct: 199 GVSFVKEKRLRVNMRLKKLQERVKEQQERVGEKIQTVK----MLRNEWVENADRWVAGFL 258

Query: 247 EMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSD--LEEEYY-----YDDDDEE 306
           E+FEEGCHKMGTAI D IQERLM  +  + LENG+D D+D    EEY+      DDD++E
Sbjct: 259 EIFEEGCHKMGTAIVDSIQERLMRQKSAERLENGQDDDTDDQFYEEYFDHDMGSDDDEDE 318

Query: 307 EYYEDGFYEEFEKDEKKR 315
           ++Y++   EE +++E ++
Sbjct: 319 KFYDE---EEVKEEETEK 322

BLAST of CmaCh01G012970 vs. TAIR 10
Match: AT4G15130.1 (phosphorylcholine cytidylyltransferase2 )

HSP 1 Score: 434.5 bits (1116), Expect = 7.3e-122
Identity = 218/276 (78.99%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 26  TDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAER 85
           +DRPVRVYADGI+DLFHFGHAR++EQAKKSFPNTYLLVGCCNDE T+  KGKTVM E+ER
Sbjct: 17  SDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITNKFKGKTVMTESER 76

Query: 86  YESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGK 145
           YESLRHCKWVDEVIPDAPWV++ EF+DKHKID+VAHD+LPYAD +GAG DVYEFVK IGK
Sbjct: 77  YESLRHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGAGNDVYEFVKSIGK 136

Query: 146 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF-EKRLRVNMRLKKLQE 205
           FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSR+ELGVSF EKRLRVNMRLKKLQE
Sbjct: 137 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSREELGVSFEEKRLRVNMRLKKLQE 196

Query: 206 KVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQER 265
           KVKE Q    EKIQTVAKTAGMH +EW+ENADRWVAGFLEMFEEGCHKMGTAIRD IQ+R
Sbjct: 197 KVKEQQ----EKIQTVAKTAGMHHDEWLENADRWVAGFLEMFEEGCHKMGTAIRDGIQQR 256

Query: 266 LM---GAQQQKYLENGKDSDSDLEEEYYYDDDDEEE 298
           LM     + ++ L+NG     D ++E   DD++  E
Sbjct: 257 LMRQESEENRRLLQNGLTISKDNDDEQMSDDNEFAE 288

BLAST of CmaCh01G012970 vs. TAIR 10
Match: AT2G38670.1 (phosphorylethanolamine cytidylyltransferase 1 )

HSP 1 Score: 112.8 bits (281), Expect = 4.9e-25
Identity = 62/166 (37.35%), Postives = 95/166 (57.23%), Query Frame = 0

Query: 28  RPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAERYE 87
           +PVRVY DG +D+ H+GH  +L QA+       L+VG  +DE     KG  V    ER  
Sbjct: 53  KPVRVYMDGCFDMMHYGHCNALRQARAL--GDQLVVGVVSDEEIIANKGPPVTPLHERMT 112

Query: 88  SLRHCKWVDEVIPDAPWVISQEFI----DKHKIDFVAHDSLPYADATGAGKDVYEFVKKI 147
            ++  KWVDEVI DAP+ I+++F+    D+++ID++ H   P       G D Y   KK 
Sbjct: 113 MVKAVKWVDEVISDAPYAITEDFMKKLFDEYQIDYIIHGDDPC--VLPDGTDAYALAKKA 172

Query: 148 GKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF 190
           G++K+ KRTEG+S++DI+ R++    +   R++   +SR  L   F
Sbjct: 173 GRYKQIKRTEGVSSTDIVGRMLLCVRE---RSISDTHSRSSLQRQF 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZV561.9e-12272.64Choline-phosphate cytidylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CCT1... [more]
F4JJE01.0e-12078.99Choline-phosphate cytidylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CCT2... [more]
Q9Y5K33.1e-6958.04Choline-phosphate cytidylyltransferase B OS=Homo sapiens OX=9606 GN=PCYT1B PE=1 ... [more]
Q811Q97.0e-6957.59Choline-phosphate cytidylyltransferase B OS=Mus musculus OX=10090 GN=Pcyt1b PE=1... [more]
Q9QZC47.0e-6957.59Choline-phosphate cytidylyltransferase B OS=Rattus norvegicus OX=10116 GN=Pcyt1b... [more]
Match NameE-valueIdentityDescription
A0A6J1J1685.2e-17699.37choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FJ477.0e-17398.42choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A1S3CM336.2e-16189.94choline-phosphate cytidylyltransferase 2-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1FCK67.6e-15988.55choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IPQ39.9e-15988.25choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022981108.11.1e-17599.37choline-phosphate cytidylyltransferase 2-like [Cucurbita maxima][more]
XP_022940119.11.5e-17298.42choline-phosphate cytidylyltransferase 2-like [Cucurbita moschata][more]
KAG6607965.11.9e-17298.10Choline-phosphate cytidylyltransferase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_023523876.14.2e-17297.47choline-phosphate cytidylyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
XP_038898408.14.4e-16191.90choline-phosphate cytidylyltransferase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G32260.11.3e-12372.64phosphorylcholine cytidylyltransferase [more]
AT4G15130.17.3e-12278.99phosphorylcholine cytidylyltransferase2 [more]
AT2G38670.14.9e-2537.35phosphorylethanolamine cytidylyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 192..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR10739:SF52CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE 2-LIKE ISOFORM X1coord: 22..301
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 31..163
IPR004821Cytidyltransferase-like domainPFAMPF01467CTP_transf_likecoord: 33..162
e-value: 2.1E-32
score: 112.3
IPR004821Cytidyltransferase-like domainTIGRFAMTIGR00125TIGR00125coord: 31..98
e-value: 3.9E-20
score: 69.8
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 2..248
e-value: 2.8E-84
score: 284.0
IPR045049Choline-phosphate cytidylyltransferase Pcy1-likePANTHERPTHR10739CYTIDYLYLTRANSFERASEcoord: 22..301
IPR041723CTP:phosphocholine cytidylyltransferase domainCDDcd02174CCTcoord: 28..179
e-value: 2.63994E-93
score: 271.748

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G012970.1CmaCh01G012970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006657 CDP-choline pathway
biological_process GO:0009058 biosynthetic process
molecular_function GO:0004105 choline-phosphate cytidylyltransferase activity
molecular_function GO:0031210 phosphatidylcholine binding
molecular_function GO:0003824 catalytic activity