CmaCh01G012090 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh01G012090 vs. ExPASy Swiss-Prot
Match: O23144 (Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2)

HSP 1 Score: 491.5 bits (1264), Expect = 1.5e-137
Identity = 316/638 (49.53%), Postives = 427/638 (66.93%), Query Frame = 0

Query: 26  MGVEVV---GFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEP--VK 85
           MGVEVV   GFE+   P +          K ++NG LDQ   ++API FGS  + P   +
Sbjct: 1   MGVEVVNSGGFEVAPAPFE---------GKPEKNGKLDQGKGDDAPINFGSVGELPKNAE 60

Query: 86  EQADVSSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARF 145
           E  +    S+ PK+AA+EWP  KQIH+FY ++YR Y DP IKA +DLADKE++K ++AR 
Sbjct: 61  ENNNKVVNSDAPKNAAEEWPVAKQIHSFYLVKYRSYADPKIKAKLDLADKELEKLNKART 120

Query: 146 QITETLKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFR-NANNSGR 205
            + + L+ KRAER+EL   +  LK + + F ++ +EK KE+EPL QALGK R N   S R
Sbjct: 121 GVLDKLRAKRAERSELFDLLDPLKSERKGFNTMFDEKRKEMEPLQQALGKLRSNDGGSAR 180

Query: 206 NGGLCSSEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQ 265
              +CSSEEELN+ I + QY IQHESIPL+EEKQIL+EI+ LEGTR+KVIANAAMRAK++
Sbjct: 181 GPAICSSEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIK 240

Query: 266 DSMVHKEAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTE 325
           +SM  K+ IQ QVK+MG  L+GV+KE QA+ A+I  + E LKA  D+I  L+ EL +V+E
Sbjct: 241 ESMGQKDDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTVSE 300

Query: 326 KRGRTYESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLW 385
           KR + Y ++  LR+ RDE N+ +YQ+R+++NKA+DLAA+K+I  LE LA  EVEKF+SLW
Sbjct: 301 KRDKAYSNIHDLRRQRDETNSEYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLW 360

Query: 386 NGDKAFRDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAK 445
              K FR+DYEKRIL SLD RQ+SRDGR+RNPDE+P++AP E AP +   +   +V +AK
Sbjct: 361 CSKKNFREDYEKRILQSLDSRQLSRDGRMRNPDEKPLIAP-EAAPSKATPSETEVVPKAK 420

Query: 446 EEPKPVPSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLM 505
            +P+P   + +SA K D  V     K    ++ ++ A+++   +  L K   +E  VD  
Sbjct: 421 AKPQP-KEEPVSAPKPDATVAQNTEKAKDAVKVKNVADDDDDEVYGLGKPQKEEKPVDAA 480

Query: 506 KLKEMKRVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTAS 565
             KEM++ EEIAKAK AMERKKKL EKAAAKA++RAQKEAEKK K+ ++EKKA KK    
Sbjct: 481 TAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEKK-EQEKKAKKK---- 540

Query: 566 GFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVD 625
                        +   EE  E+ E E+++   +   P K  + KE  IR+R  + RG +
Sbjct: 541 --------TGGNTETETEEVPEASE-EEIEAPVQEEKPQKEKVFKEKPIRNR-TRGRGPE 600

Query: 626 SIPKVIRKRKKANNYWVWAAPAAAAALLVILLLLGYYY 658
           +IP+ I KRKK+ NYWV+AAPAA   LL  LL+LGYYY
Sbjct: 601 TIPRAILKRKKSTNYWVYAAPAALVVLL--LLVLGYYY 610

BLAST of CmaCh01G012090 vs. ExPASy Swiss-Prot
Match: Q6ZBF6 (Proton pump-interactor BIP131 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP131 PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 4.1e-119
Identity = 296/650 (45.54%), Postives = 412/650 (63.38%), Query Frame = 0

Query: 28  VEVVGFEMVEGPVDSVSLADN------SVSKSKENGDLDQLPEENAPIKFGSHEDEPVKE 87
           +E  G E     V +V   DN      S + +KE G+      EN     G+        
Sbjct: 1   MEATGAEATLSQVTAVDGEDNLFQDKESRATAKERGEAAVFGLENIVTANGA------TS 60

Query: 88  QADVSSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQ 147
            AD++     PKD  DEWP PKQ HTF+F+R   Y+DP++KA ++ ADKE QK+ QAR  
Sbjct: 61  AADLAP----PKDVVDEWPEPKQTHTFFFVRICSYEDPSLKAKLEQADKECQKKIQARSH 120

Query: 148 ITETLKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGR-- 207
           I E L+ KR+ER+ +I++++ L  +N+Q+  +V  K+KEIEPL ++LGKFR+ NN+ R  
Sbjct: 121 IFEALRTKRSERSNIISELKPLAAENKQYNEVVSGKLKEIEPLQKSLGKFRSENNAMRAQ 180

Query: 208 NGGLCSSEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQ 267
             GLCSS EEL+  I++L   I HESI L EEK++++EIKQL GTR KVI NAA RAK+Q
Sbjct: 181 GAGLCSSIEELDQLIKSLNDRISHESISLDEEKRLVKEIKQLNGTRSKVIENAAKRAKMQ 240

Query: 268 DSMVHKEAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTE 327
           D++V +  I DQVK +G  ++ V+++ QAVR KIK +E+ + A+D +I  LQ++LT+ T 
Sbjct: 241 DTVVERGTIHDQVKQIGVGIDEVKRDRQAVRDKIKVLEDQIHAVDGEIAALQDDLTAATA 300

Query: 328 KRGRTYESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLW 387
           ++ + +E++ +LRK RD  N  F+Q R+I N  +DL+A+ +++A+++L  +EVEKFM+ W
Sbjct: 301 RKDKAFEALNELRKTRDLNNTSFHQYRTISNSVRDLSARGEVEAVQQLCQNEVEKFMAQW 360

Query: 388 NGDKAFRDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPI---VK 447
              K+FR+DYEKRIL SL+ RQ+SRDGR+RNPDE+PI+   ++APP  E  P P+    K
Sbjct: 361 CSSKSFREDYEKRILVSLNSRQLSRDGRMRNPDEKPIVLETQVAPP-AEQEPAPLKKPAK 420

Query: 448 RAKEEPKPVPSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKE--- 507
           +AKE P P  +D     ++  +   KA K  +PL+ +D     IP +     E PKE   
Sbjct: 421 QAKEAPAP-RADVTPKDEIRAKAPAKAAKAKQPLDIDD-----IPDVH--DDEPPKEKTK 480

Query: 508 PEVDLMKLKEMKRVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAM 567
           P+VD  KLKEMKR EEI K KLA+ERKKK  EK A KA+ RA+KEAEKKLKE  +EKKA 
Sbjct: 481 PKVDEAKLKEMKRQEEIEKNKLALERKKKQAEKQAMKAAARAEKEAEKKLKE--KEKKAR 540

Query: 568 KKMTASGFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGN 627
           K+   +G A    EE+A++DA      +S E E  +E   APV     I+K  R RS   
Sbjct: 541 KRSATAGGA--ESEEAAESDA------KSDEAEAQEEEPAAPV----TIKKNARHRSTVT 600

Query: 628 KQRGVDSIPKVIRKRKKANNYWVWAAP-AAAAALLVILL--LLGYYYYYL 661
           K +    +PK + KRKK+  +W W AP AA AA LV LL  L+ Y YYYL
Sbjct: 601 KTK--TPLPKAVLKRKKSQAFWSWGAPMAALAAALVALLGALVYYQYYYL 615

BLAST of CmaCh01G012090 vs. ExPASy Swiss-Prot
Match: Q762B4 (Proton pump-interactor BIP103 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP103 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 7.7e-118
Identity = 285/646 (44.12%), Postives = 405/646 (62.69%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MG+EVVG E        V + D  V+  +EN       E    + FGS         A  
Sbjct: 1   MGMEVVGTEAAPA---EVKVTDGEVNLFQENESKATAKEREEAVLFGSDNSTATANGAAN 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
            +    PKDA ++WP  ++ H+F+F++ R  +DP +K  ID A+K+ QK+ QAR QI E 
Sbjct: 61  GADLAPPKDAEEDWPEARKTHSFFFVKIRLLEDPKLKMKIDQAEKDFQKKIQARSQIFEA 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNS--GRNGGL 205
           +K K+ ER  +I++++ L  +N+Q+   V EK+K IEPL   LGKFR+ NN+   +  G+
Sbjct: 121 IKAKKNERFGIISELKPLAAENKQYNEAVSEKLKAIEPLRNRLGKFRDENNAIRAQGAGI 180

Query: 206 CSSEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMV 265
           CSS EEL  SI+ L   I HESIPL EEK+++++I++LE TR KVI+ +A RA++QD++V
Sbjct: 181 CSSIEELEKSIKRLNDRISHESIPLDEEKRLIKQIRELEKTRPKVISTSANRAQIQDTVV 240

Query: 266 HKEAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGR 325
            ++AIQDQVKI+G  ++GV+KE QAVR+KIK +E+ LKAID ++ +LQE+LT+   ++ +
Sbjct: 241 ERDAIQDQVKIIGEGIDGVKKERQAVRSKIKVLEDELKAIDMEMGSLQEDLTAANARKDK 300

Query: 326 TYESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDK 385
            +ES+ QLR  RD  NA F+Q+R +++KA+DLA++ ++  ++EL   +V+KF++ W   K
Sbjct: 301 AHESLVQLRHARDAYNASFHQNRQLLSKARDLASRSELAQVQELYKTQVDKFVAEWCNSK 360

Query: 386 AFRDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAP---KPIVKRAKE 445
           AFR+DYEKRIL+SL+ RQ+SRDGR+RNPDE+PI    E A P  E  P   K   K+AKE
Sbjct: 361 AFREDYEKRILSSLNSRQLSRDGRMRNPDEKPIFIETEAAAPPVEQEPIQSKMPAKQAKE 420

Query: 446 EPKP----VPSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEV 505
            P P     P D    + + K  K K+      L+ +D  E E P      KE PK  EV
Sbjct: 421 APAPQAEVSPKDESRVKAIAKPSKAKSS-----LDADDDYEAESP------KEKPKPKEV 480

Query: 506 DLMKLKEMKRVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKM 565
           D+ KLKE+KR EE+ K +LA+ERKKKL EK AAKA+ RAQKEAEKKLK    E +A ++ 
Sbjct: 481 DVAKLKEIKRQEEMEKNRLALERKKKLAEKQAAKAAARAQKEAEKKLKR--EEMRARRRA 540

Query: 566 TASGFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQR 625
            A+       E S ++D     S  + E +  D++A A  P     Q+E+   SR    +
Sbjct: 541 GAA-----DTEASTESD---NRSDGAAEAQAEDDSAPASAPVMRE-QRESVRYSRNVVTK 600

Query: 626 GVDSIPKVIRKRKKANNYWVWAAPAA--AAALLVILLLLGYYYYYL 661
               +PK I +RKKA +YW WA PAA  AAAL+ +L +LGYY YYL
Sbjct: 601 SKAPLPKAILRRKKAQSYWSWAGPAAAVAAALVALLAVLGYYQYYL 621

BLAST of CmaCh01G012090 vs. ExPASy Swiss-Prot
Match: B3H4K7 (Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 6.6e-69
Identity = 218/608 (35.86%), Postives = 326/608 (53.62%), Query Frame = 0

Query: 63  PEENAPIKFGSHE-------DEPVKEQADVSSASNVPKDAADEWPAPKQIHTFYFIRYRP 122
           PE N  + FGS++            E   V S  + P  A +E    ++  +FYF++   
Sbjct: 17  PEMNDLVLFGSNQSVSSCDVSTVTTEDGTVFSGDSSPGGATEEDFPEEKPFSFYFVKQPA 76

Query: 123 YDDPNIKANIDLADKEIQKRSQARFQITETLKGKRAERAELITQVRALKDDNRQFKSIVE 182
           YDDP IKA ID A  EI + ++ R  ++   + ++AE   L  Q+++L   +  ++ ++E
Sbjct: 77  YDDPEIKAKIDEAGHEINRYNKDRIVVSNAQESEKAEILSLFGQMKSLVSKSEGYRVVIE 136

Query: 183 EKIKEIEPLNQALGKFRNANNSGRNGGLCSSEEELNASIQALQYHIQHESIPLSEEKQIL 242
           EK  E + L+++L   R + +      LC S+EEL+  I    Y I+H SI   EE  +L
Sbjct: 137 EKKMEFDALHESLRNLRCSTSD----QLCFSKEELDHLIYIAHYQIEHGSIGFEEEDWVL 196

Query: 243 REIKQLEGTREKVIANAAMRAKLQDSMVHKEAIQDQVKIMGGDLNGVRKEHQAVRAKIKH 302
           +E ++ +G               +DS+  KEA  ++VK M  +LN V+KE  A+  KI H
Sbjct: 197 KETEKADGI-----------VLSEDSLAEKEASINRVKSMAVELNEVKKELDAITWKINH 256

Query: 303 IEEALKAIDDDIKTLQEELTSVTEKRGRTYESMQQLRKNRDEGNAHFYQSRSIMNKAKDL 362
           + + +    ++++ L  +   + E+R R+YE ++ LR  RD+G A FYQS  +M KA++L
Sbjct: 257 LSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMRKAREL 316

Query: 363 AAKKDIKALEELACDEVEKFMSLWNGDKAFRDDYEKRILASLDVRQMSRDGRIRNPDERP 422
           AA  +++ LE  A  E ++FM+ WN DKAFRDDY KRI  SL  RQ+++DGRI++P+ + 
Sbjct: 317 AASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRIKDPEVQV 376

Query: 423 ILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSDTLSAQKVDKEVKPKAGKPSR-PLEKED 482
           +    E   P           R           ++   K  KE + KA   +R   E+ D
Sbjct: 377 VW---EKKVPVKGGEKVHETNREDSSSNSSQYGSVITDKRKKETRKKAMDFNRSSAEESD 436

Query: 483 KAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVEEIAKAKLAMERKKKLQEKAAAKASLR 542
             + E    EK  KE   E EVD   LKE +R E++ KA+LAMERK+KLQEKAAAKA++R
Sbjct: 437 VTDLEFSVYEKPKKE---EEEVDEETLKEREREEQLEKARLAMERKRKLQEKAAAKAAIR 496

Query: 543 AQKEAEKKLKEIDREKKAMKKMTASGFAFLPDEESAQADAVEEESKESKELEKVDENAEA 602
           AQKEAEKKLKE   EKKA KK  A+         S+     +   + +K+LEKV   A  
Sbjct: 497 AQKEAEKKLKEC--EKKAKKKAAAN---------SSSPSESDHSQEVTKDLEKVRTLA-- 556

Query: 603 PVPAKTNIQKET---------RIRSRGNKQRGVDSIPKVIRKRKKANNYWVWAAPAAAAA 654
            V  K   QKE          R + RG   RG +++PK I  R+KA+ YWVW   +AA A
Sbjct: 557 -VSGKEKHQKERSLFPKQRSFRYKHRG---RGTEALPKAILNRRKAHKYWVWGLSSAALA 586

BLAST of CmaCh01G012090 vs. ExPASy Swiss-Prot
Match: P0DKC1 (Proton pump-interactor 3A OS=Arabidopsis thaliana OX=3702 GN=PPI3A PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.5e-60
Identity = 216/634 (34.07%), Postives = 330/634 (52.05%), Query Frame = 0

Query: 32  GFEMVEGP-VDSVSL--ADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADVSSA 91
           GFE+V  P ++ + L   D S++ S+   D                E++ +    D SS 
Sbjct: 10  GFEVVPSPEINDLILYECDQSLTNSEVTTD---------------EEEDTIFSGGDSSSG 69

Query: 92  SNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITETLKG 151
               +D++ + P      +FY ++   YD+P IKA ID A++EI + ++ R  +    K 
Sbjct: 70  LAAEEDSSGDKPL-----SFYIVKQPVYDNPEIKAKIDEANQEIFRCNELRINVLSAKKS 129

Query: 152 KRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCSSEE 211
           + AE + L TQ+ +L   +  ++ ++EEK KE + L +AL   R   +      LC ++E
Sbjct: 130 ELAEVSSLYTQMESLVPQSEGYRMVIEEKKKEFDTLLEALRNLRCTTSD----QLCFTKE 189

Query: 212 ELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAI 271
           EL+      QY I++ SI L EE  +L+E ++ +G                  +  KEA 
Sbjct: 190 ELDHLSYIAQYQIEYGSIGLEEEDWMLKETEKPDGI----------------ILSEKEAS 249

Query: 272 QDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTYESM 331
            ++VK M  +LN V+ E  A+  KI  + + L    ++I+ L  E   + EKR R Y  +
Sbjct: 250 INRVKSMALELNEVKNELDAITWKINDLSDKLWKSQNNIRVLDLEKAHILEKRDRFYARI 309

Query: 332 QQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDD 391
           + LR  RD+GNA F+QS S+M KAK+LAA  +++ LE  A  EV++FM+LWN DKAFR+D
Sbjct: 310 KMLRIQRDKGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDRFMTLWNDDKAFRED 369

Query: 392 YEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSD 451
           Y +RI  SL  R+++ DGRI++ D         L     +  P   +KR+++  K    D
Sbjct: 370 YVRRISHSLCERELNEDGRIKDAD---------LQIFWEKKVPVKTIKRSEKVHKMNRED 429

Query: 452 TLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVE 511
           + S    D  V     K      KE K++  +   EK  K   KE E+D   LKE KR E
Sbjct: 430 SSSNSSEDGNVITDKRK------KETKSDVIV--YEKPKK---KEEEIDEEALKERKREE 489

Query: 512 EIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEI---------DREKKAMKKMTAS 571
           ++ KA+L MERK+KLQEKAAAKA++RAQKEAEKKLK I         + EKKA KK  A+
Sbjct: 490 QLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFFNECEKKAKKKAAAN 549

Query: 572 GFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVD 631
             +    ++    + V   +   KE  +  E    P       Q+  R + RG+   G +
Sbjct: 550 STSPSESDQVINDEKVRTLAVSGKEKHQ-KERLLFPK------QRSFRYKHRGS---GTE 573

Query: 632 SIPKVIRKRKKANNYWVWAAPAAAAALLVILLLL 654
           ++PK I  R+KA  YWVW   +AA A+ ++L++L
Sbjct: 610 ALPKAILNRRKARRYWVWGLSSAALAVSLVLVVL 573

BLAST of CmaCh01G012090 vs. ExPASy TrEMBL
Match: A0A6J1J0D4 (proton pump-interactor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480768 PE=4 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 634/636 (99.69%), Postives = 634/636 (99.69%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV
Sbjct: 1   MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET
Sbjct: 61  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS
Sbjct: 121 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK
Sbjct: 181 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY
Sbjct: 241 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF
Sbjct: 301 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV
Sbjct: 361 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK
Sbjct: 421 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLP 565
           RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKAMKKMTASGFAFLP
Sbjct: 481 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAMKKMTASGFAFLP 540

Query: 566 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 625
           DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI
Sbjct: 541 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 600

Query: 626 RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 662
           RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI
Sbjct: 601 RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 634

BLAST of CmaCh01G012090 vs. ExPASy TrEMBL
Match: A0A6J1FQY0 (proton pump-interactor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446437 PE=4 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 620/637 (97.33%), Postives = 627/637 (98.43%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQ DV
Sbjct: 1   MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQPDV 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SSASNVPKDAADEWPAPKQIHTFYFIRYRPY+DPNIKA IDLADKEIQKRSQARFQITET
Sbjct: 61  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYEDPNIKAKIDLADKEIQKRSQARFQITET 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANN GRNGGLCS
Sbjct: 121 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNPGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK
Sbjct: 181 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EAIQDQVKIMGGDLNGVRKEHQAVRAKIK IEEALK IDDDIKTLQ+ELTSVTEKRGRTY
Sbjct: 241 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKQIEEALKTIDDDIKTLQDELTSVTEKRGRTY 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ESMQQLRKNRDEGNAHFYQSRSIM+KAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF
Sbjct: 301 ESMQQLRKNRDEGNAHFYQSRSIMSKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RDDYEKRILASLDVRQMSRDGRIRNPDE+PILAPVELAPPRTEAAPKPIVKRAKEEPKPV
Sbjct: 361 RDDYEKRILASLDVRQMSRDGRIRNPDEKPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTL AQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSK+IPKEPEVDL+KLKEMK
Sbjct: 421 PSDTLPAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKDIPKEPEVDLVKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLP 565
           RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKAMKKMTASGFA LP
Sbjct: 481 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAMKKMTASGFASLP 540

Query: 566 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 625
           DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRG+KQRGVDSIPKVI
Sbjct: 541 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGSKQRGVDSIPKVI 600

Query: 626 RKRKKANNYWVWAAP-AAAAALLVILLLLGYYYYYLI 662
           RKRKKANNYWVWAAP AAAAALLVILLLLGYYYYYLI
Sbjct: 601 RKRKKANNYWVWAAPAAAAAALLVILLLLGYYYYYLI 635

BLAST of CmaCh01G012090 vs. ExPASy TrEMBL
Match: A0A6J1F7E9 (proton pump-interactor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442817 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.5e-273
Identity = 532/638 (83.39%), Postives = 581/638 (91.07%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQAD- 85
           MGVEVVGFEMV+GP+DS  +ADN V KS ENGDLDQLPE+N PIKFGSHE+EPVKEQAD 
Sbjct: 1   MGVEVVGFEMVKGPMDSAPVADNYVLKSDENGDLDQLPEKNVPIKFGSHEEEPVKEQADG 60

Query: 86  VSSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITE 145
            SSASNVPKDAADEWPAPKQIH+FYFIR+RPYDDP+IKA IDLADKEI+KRSQARFQITE
Sbjct: 61  SSSASNVPKDAADEWPAPKQIHSFYFIRHRPYDDPSIKAKIDLADKEIRKRSQARFQITE 120

Query: 146 TLKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLC 205
           TLK KRAERAELITQ++ L+DDNRQ+KSIV+EKIKEIEPLNQALGKFRNANN+GRNGGLC
Sbjct: 121 TLKSKRAERAELITQLKPLRDDNRQYKSIVDEKIKEIEPLNQALGKFRNANNAGRNGGLC 180

Query: 206 SSEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVH 265
           SSEEELNA IQ+LQYHIQHESIPLSEEKQILREIKQLEGTREKVIAN+AMRAKLQDSMVH
Sbjct: 181 SSEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANSAMRAKLQDSMVH 240

Query: 266 KEAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRT 325
           KEA+QDQVKI+GGDLNGVRKE QAVRAKIK +EEAL+AID+DIKTLQ+ELTSVTEKRG+ 
Sbjct: 241 KEALQDQVKIIGGDLNGVRKEQQAVRAKIKQLEEALEAIDEDIKTLQDELTSVTEKRGKA 300

Query: 326 YESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKA 385
           +E++QQLRKN DEGNA FYQSRS++NKAKDLAAKKDI+ALEELA  EVEKFMSLWNGDKA
Sbjct: 301 HENIQQLRKNSDEGNAQFYQSRSLLNKAKDLAAKKDIQALEELAFTEVEKFMSLWNGDKA 360

Query: 386 FRDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKP 445
           FRDDYEKRILASLD+RQMSRDGRIRNPDE+PILAP E AP +TE A KP +KR KEEPKP
Sbjct: 361 FRDDYEKRILASLDIRQMSRDGRIRNPDEKPILAPAEPAPRQTEMAAKPNIKRTKEEPKP 420

Query: 446 VPSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEM 505
           VPSD L AQKVD EVK KAGKP RPLE+EDKAEEEIP L KLSK+IPKEPEVDL KLKEM
Sbjct: 421 VPSDPLPAQKVDIEVKQKAGKPVRPLEQEDKAEEEIPSLLKLSKDIPKEPEVDLAKLKEM 480

Query: 506 KRVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFL 565
           KR EEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKA KK+TASG A +
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAKKKVTASGSATV 540

Query: 566 PDEESAQADAVEEESKESKELEKVDENAE-APVPAKTNIQKETRIRSRGNKQRGVDSIPK 625
           PDEE  +ADAVEE   ES ELE V+E+AE APVP K+ I KET IRSRG +QRG+DS+PK
Sbjct: 541 PDEEPVEADAVEE---ESTELENVNESAEAAPVPVKSKIPKETGIRSRG-RQRGLDSVPK 600

Query: 626 VIRKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 662
           +IRKRKK+ NYW WAAP AAA LLVILL+LGYYYYYL+
Sbjct: 601 IIRKRKKSINYWDWAAP-AAAVLLVILLVLGYYYYYLL 631

BLAST of CmaCh01G012090 vs. ExPASy TrEMBL
Match: A0A6J1IM70 (proton pump-interactor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476847 PE=4 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 1.3e-272
Identity = 530/638 (83.07%), Postives = 580/638 (90.91%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQAD- 85
           MGVEVVGFEMV+GP+DS  +ADN V KS ENGDLDQLPE+N PIKFGSHE+EPVKEQAD 
Sbjct: 1   MGVEVVGFEMVKGPMDSAPVADNYVLKSDENGDLDQLPEKNVPIKFGSHEEEPVKEQADG 60

Query: 86  VSSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITE 145
            SSASNVPKDAADEWPAPKQIH+FYFIR+RPYDDP+IKA IDLADKEI+KRSQARFQITE
Sbjct: 61  SSSASNVPKDAADEWPAPKQIHSFYFIRHRPYDDPSIKAKIDLADKEIRKRSQARFQITE 120

Query: 146 TLKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLC 205
           TLK KRAERAELITQ++ L+DDNRQ+KSIV+EKIKEIEPLNQALGKFRNANN+GRNGGLC
Sbjct: 121 TLKSKRAERAELITQLKPLRDDNRQYKSIVDEKIKEIEPLNQALGKFRNANNAGRNGGLC 180

Query: 206 SSEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVH 265
           SSEEELNA IQ+LQYHIQHESIPLSEEKQILREIKQLEGTREKVIAN+AMRAKLQDSMVH
Sbjct: 181 SSEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANSAMRAKLQDSMVH 240

Query: 266 KEAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRT 325
           KEA+QDQVKI+GGDLNGVRKE QAVRAKIK +EEAL+AID+DIKTLQ+ELTSVTEKRG+ 
Sbjct: 241 KEALQDQVKIIGGDLNGVRKEQQAVRAKIKQLEEALEAIDEDIKTLQDELTSVTEKRGKA 300

Query: 326 YESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKA 385
           +E++QQLRKN DEGNA FYQSRS++NKAKDLAAKKDI+ALEELA  EVEKFMSLWNGDKA
Sbjct: 301 HENIQQLRKNSDEGNAQFYQSRSLLNKAKDLAAKKDIQALEELAFTEVEKFMSLWNGDKA 360

Query: 386 FRDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKP 445
           FRDDYEKRILASLD+RQMSRDGRIRNPDE+PILAP + AP +TE A KP +KR KEEPKP
Sbjct: 361 FRDDYEKRILASLDIRQMSRDGRIRNPDEKPILAPADPAPRQTEMAAKPNIKRTKEEPKP 420

Query: 446 VPSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEM 505
           VPSD L AQKVD EVK KAGKP RPLE+EDKAEEEIP L KLSK+IPKEPEVDL KLKEM
Sbjct: 421 VPSDPLPAQKVDIEVKQKAGKPVRPLEQEDKAEEEIPSLLKLSKDIPKEPEVDLAKLKEM 480

Query: 506 KRVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFL 565
           KR EEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKA KK+TASG A +
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAKKKVTASGSAMV 540

Query: 566 PDEESAQADAVEEESKESKELEKVDENAE-APVPAKTNIQKETRIRSRGNKQRGVDSIPK 625
           PDEE  +A AVEE   ES ELE V+E+AE APVP K+ I KET IRSRG +QRG+DS+PK
Sbjct: 541 PDEEPVEAAAVEE---ESTELENVNESAEAAPVPVKSKIPKETSIRSRG-RQRGLDSVPK 600

Query: 626 VIRKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 662
           +IRKRKK+ NYW WAAP AAA LLVILL+LGYYYYYL+
Sbjct: 601 IIRKRKKSINYWDWAAP-AAAVLLVILLVLGYYYYYLL 631

BLAST of CmaCh01G012090 vs. ExPASy TrEMBL
Match: A0A0A0L261 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637150 PE=4 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 8.2e-272
Identity = 531/646 (82.20%), Postives = 577/646 (89.32%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMV GP+DS   ADNSV KSKENGDLDQLPE+NA IKFGSHEDEPVKEQAD 
Sbjct: 1   MGVEVVGFEMVNGPMDS---ADNSVLKSKENGDLDQLPEKNATIKFGSHEDEPVKEQADG 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SS +NVPKDAADEWPAPKQIHTFYF+R+R YDDPN+KA IDLADKEIQKRSQARFQITE 
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKR ERAELITQ++AL+DDNRQFKSIV+EKIKEIEPLNQALGK RNANN+GRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNA IQ+LQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMV K
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EA+QDQVKI+GGDL+GVRKE QAVRAKIK +++ALKAID++IKTLQ+ELTSVTEKRGR +
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ES+QQLRKNRDEGNAHFYQSRS++NKA+DLAA+KD+KALEELA +EVEKFMSLWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELASNEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RD+YEKRIL SLD RQMSRDGRIRNPDE+PILAP E APP+TE A KP +KR KEEPKPV
Sbjct: 361 RDEYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTL AQKVDKEVK K GK +RPLE+EDKAE+EI GLEKLSK+IPKEPEVDL KLKEMK
Sbjct: 421 PSDTLPAQKVDKEVKHKVGKSARPLEQEDKAEDEIHGLEKLSKDIPKEPEVDLAKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKE-------IDREKKAMKKMTA 565
           R EEIAKAKLAMERKKKLQEKAA KASLRAQKEAEKKLKE       IDREKKA KK T 
Sbjct: 481 RAEEIAKAKLAMERKKKLQEKAAEKASLRAQKEAEKKLKEIIYIHTHIDREKKAKKKATT 540

Query: 566 SG-FAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRG 625
           SG FA LPDEE   ADAVEE   ES E+EKV+E AEAPVP KT I KET +R R  +QRG
Sbjct: 541 SGSFATLPDEEPTDADAVEE---ESTEVEKVNETAEAPVPVKTKIPKETVVR-RPGRQRG 600

Query: 626 VDSIPKVIRKRKKANNYWVWAAPAA--AAALLVILLLLGYYYYYLI 662
           +DS+PKVIRKRKK+ NY  WA PAA  AA LLVILL+LGYYYYYL+
Sbjct: 601 LDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 639

BLAST of CmaCh01G012090 vs. NCBI nr
Match: XP_022981705.1 (proton pump-interactor 1-like [Cucurbita maxima] >XP_022981706.1 proton pump-interactor 1-like [Cucurbita maxima])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 634/636 (99.69%), Postives = 634/636 (99.69%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV
Sbjct: 1   MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET
Sbjct: 61  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS
Sbjct: 121 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK
Sbjct: 181 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY
Sbjct: 241 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF
Sbjct: 301 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV
Sbjct: 361 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK
Sbjct: 421 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLP 565
           RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKAMKKMTASGFAFLP
Sbjct: 481 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAMKKMTASGFAFLP 540

Query: 566 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 625
           DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI
Sbjct: 541 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 600

Query: 626 RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 662
           RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI
Sbjct: 601 RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 634

BLAST of CmaCh01G012090 vs. NCBI nr
Match: XP_023524719.1 (proton pump-interactor 1-like [Cucurbita pepo subsp. pepo] >XP_023524720.1 proton pump-interactor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 621/636 (97.64%), Postives = 626/636 (98.43%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQ DV
Sbjct: 1   MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQPDV 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SSASNVPKDAADEWPAPKQIHTFYFIRYRPY+DPNIKA IDLADKEIQKRSQARFQITET
Sbjct: 61  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYEDPNIKAKIDLADKEIQKRSQARFQITET 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANN GRNGGLCS
Sbjct: 121 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNPGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK
Sbjct: 181 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EAIQDQVKIMGGDLNGVRKEHQAVRAKIK IEEALK IDDDIKTLQ+ELTSVTEKRGRTY
Sbjct: 241 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKQIEEALKTIDDDIKTLQDELTSVTEKRGRTY 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF
Sbjct: 301 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RDDYEKRILASLDVRQMSRDGRIRNPDE+PILAPVELAPPRTEAAPKP VKRAKEEPKPV
Sbjct: 361 RDDYEKRILASLDVRQMSRDGRIRNPDEKPILAPVELAPPRTEAAPKPFVKRAKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTL AQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSK+IPKEPEVDL+KLKEMK
Sbjct: 421 PSDTLPAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKDIPKEPEVDLVKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLP 565
           RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKAMKKMTASGFA LP
Sbjct: 481 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAMKKMTASGFASLP 540

Query: 566 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 625
           DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI
Sbjct: 541 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 600

Query: 626 RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 662
           RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI
Sbjct: 601 RKRKKANNYWVWAAPAAAAALLVILLLLGYYYYYLI 634

BLAST of CmaCh01G012090 vs. NCBI nr
Match: KAG7037408.1 (Dynamin-related protein 5A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 616/629 (97.93%), Postives = 621/629 (98.73%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQ DV
Sbjct: 1   MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQPDV 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SSASNVPKDAADEWPAPKQIHTFYFIRYRPY+DPNIKA IDLADKEIQKRSQARFQITET
Sbjct: 61  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYEDPNIKAKIDLADKEIQKRSQARFQITET 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANN GRNGGLCS
Sbjct: 121 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNPGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK
Sbjct: 181 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EAIQDQVKIMGGDLNGVRKEHQAVRAKIK IEEALK IDDDIKTLQ+ELTSVTEKRGRTY
Sbjct: 241 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKQIEEALKTIDDDIKTLQDELTSVTEKRGRTY 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF
Sbjct: 301 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RDDYEKRILASLDVRQMSRDGRIRNPDE+PILAPVELAPPRTEAAPKP VKRAKEEPKPV
Sbjct: 361 RDDYEKRILASLDVRQMSRDGRIRNPDEKPILAPVELAPPRTEAAPKPFVKRAKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTL AQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSK+IPKEPEVDL+KLKEMK
Sbjct: 421 PSDTLPAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKDIPKEPEVDLVKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLP 565
           RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFA LP
Sbjct: 481 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFASLP 540

Query: 566 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 625
           DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI
Sbjct: 541 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 600

Query: 626 RKRKKANNYWVWAAPAAAAALLVILLLLG 655
           RKRKKANNYWVWAAPAAAAALLVILLLLG
Sbjct: 601 RKRKKANNYWVWAAPAAAAALLVILLLLG 629

BLAST of CmaCh01G012090 vs. NCBI nr
Match: XP_022941025.1 (proton pump-interactor 1-like [Cucurbita moschata] >XP_022941028.1 proton pump-interactor 1-like [Cucurbita moschata])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 620/637 (97.33%), Postives = 627/637 (98.43%), Query Frame = 0

Query: 26  MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADV 85
           MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQ DV
Sbjct: 1   MGVEVVGFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQPDV 60

Query: 86  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITET 145
           SSASNVPKDAADEWPAPKQIHTFYFIRYRPY+DPNIKA IDLADKEIQKRSQARFQITET
Sbjct: 61  SSASNVPKDAADEWPAPKQIHTFYFIRYRPYEDPNIKAKIDLADKEIQKRSQARFQITET 120

Query: 146 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCS 205
           LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANN GRNGGLCS
Sbjct: 121 LKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNPGRNGGLCS 180

Query: 206 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 265
           SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK
Sbjct: 181 SEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 266 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTY 325
           EAIQDQVKIMGGDLNGVRKEHQAVRAKIK IEEALK IDDDIKTLQ+ELTSVTEKRGRTY
Sbjct: 241 EAIQDQVKIMGGDLNGVRKEHQAVRAKIKQIEEALKTIDDDIKTLQDELTSVTEKRGRTY 300

Query: 326 ESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 385
           ESMQQLRKNRDEGNAHFYQSRSIM+KAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF
Sbjct: 301 ESMQQLRKNRDEGNAHFYQSRSIMSKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAF 360

Query: 386 RDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 445
           RDDYEKRILASLDVRQMSRDGRIRNPDE+PILAPVELAPPRTEAAPKPIVKRAKEEPKPV
Sbjct: 361 RDDYEKRILASLDVRQMSRDGRIRNPDEKPILAPVELAPPRTEAAPKPIVKRAKEEPKPV 420

Query: 446 PSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMK 505
           PSDTL AQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSK+IPKEPEVDL+KLKEMK
Sbjct: 421 PSDTLPAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKDIPKEPEVDLVKLKEMK 480

Query: 506 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLP 565
           RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK  DREKKAMKKMTASGFA LP
Sbjct: 481 RVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK--DREKKAMKKMTASGFASLP 540

Query: 566 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVI 625
           DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRG+KQRGVDSIPKVI
Sbjct: 541 DEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGSKQRGVDSIPKVI 600

Query: 626 RKRKKANNYWVWAAP-AAAAALLVILLLLGYYYYYLI 662
           RKRKKANNYWVWAAP AAAAALLVILLLLGYYYYYLI
Sbjct: 601 RKRKKANNYWVWAAPAAAAAALLVILLLLGYYYYYLI 635

BLAST of CmaCh01G012090 vs. NCBI nr
Match: KAG6607879.1 (Proton pump-interactor 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 612/627 (97.61%), Postives = 617/627 (98.41%), Query Frame = 0

Query: 35  MVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADVSSASNVPKD 94
           MVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQ DVSSASNVPKD
Sbjct: 1   MVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQPDVSSASNVPKD 60

Query: 95  AADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITETLKGKRAERA 154
           AADEWPAPKQIHTFYFIRYRPY+DPNIKA IDLADKEIQKRSQARFQITETLKGKRAERA
Sbjct: 61  AADEWPAPKQIHTFYFIRYRPYEDPNIKAKIDLADKEIQKRSQARFQITETLKGKRAERA 120

Query: 155 ELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCSSEEELNASI 214
           ELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANN GRNGGLCSSEEELNASI
Sbjct: 121 ELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNPGRNGGLCSSEEELNASI 180

Query: 215 QALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAIQDQVKI 274
           QALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAIQDQVKI
Sbjct: 181 QALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAIQDQVKI 240

Query: 275 MGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTYESMQQLRKN 334
           MGGDLNGVRKEHQAVRAKIK IEEALK IDDDIKTLQ+ELTSVTEKRGRTYESMQQLRKN
Sbjct: 241 MGGDLNGVRKEHQAVRAKIKQIEEALKTIDDDIKTLQDELTSVTEKRGRTYESMQQLRKN 300

Query: 335 RDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDDYEKRIL 394
           RDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDDYEKRIL
Sbjct: 301 RDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDDYEKRIL 360

Query: 395 ASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSDTLSAQK 454
           ASLDVRQMSRDGRIRNPDE+PILAPVELAPPRTEAAPKP VKRAKEEPKPVPSDTL AQK
Sbjct: 361 ASLDVRQMSRDGRIRNPDEKPILAPVELAPPRTEAAPKPFVKRAKEEPKPVPSDTLPAQK 420

Query: 455 VDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVEEIAKAK 514
           VDKEVKPKAGKPSRPLEKEDKAEE I GLEKLSK+IPKEPEVDL+KLKEMKRVEEIAKAK
Sbjct: 421 VDKEVKPKAGKPSRPLEKEDKAEEVILGLEKLSKDIPKEPEVDLVKLKEMKRVEEIAKAK 480

Query: 515 LAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLPDEESAQADA 574
           LAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFA LPDEESAQADA
Sbjct: 481 LAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFASLPDEESAQADA 540

Query: 575 VEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVIRKRKKANNY 634
           VEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVIRKRKKANNY
Sbjct: 541 VEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVDSIPKVIRKRKKANNY 600

Query: 635 WVWAAPAAAAALLVILLLLGYYYYYLI 662
           WVWAAPAAAAALLVILLLLGYYYYYLI
Sbjct: 601 WVWAAPAAAAALLVILLLLGYYYYYLI 627

BLAST of CmaCh01G012090 vs. TAIR 10
Match: AT4G27500.1 (proton pump interactor 1 )

HSP 1 Score: 491.5 bits (1264), Expect = 1.1e-138
Identity = 316/638 (49.53%), Postives = 427/638 (66.93%), Query Frame = 0

Query: 26  MGVEVV---GFEMVEGPVDSVSLADNSVSKSKENGDLDQLPEENAPIKFGSHEDEP--VK 85
           MGVEVV   GFE+   P +          K ++NG LDQ   ++API FGS  + P   +
Sbjct: 1   MGVEVVNSGGFEVAPAPFE---------GKPEKNGKLDQGKGDDAPINFGSVGELPKNAE 60

Query: 86  EQADVSSASNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARF 145
           E  +    S+ PK+AA+EWP  KQIH+FY ++YR Y DP IKA +DLADKE++K ++AR 
Sbjct: 61  ENNNKVVNSDAPKNAAEEWPVAKQIHSFYLVKYRSYADPKIKAKLDLADKELEKLNKART 120

Query: 146 QITETLKGKRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFR-NANNSGR 205
            + + L+ KRAER+EL   +  LK + + F ++ +EK KE+EPL QALGK R N   S R
Sbjct: 121 GVLDKLRAKRAERSELFDLLDPLKSERKGFNTMFDEKRKEMEPLQQALGKLRSNDGGSAR 180

Query: 206 NGGLCSSEEELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQ 265
              +CSSEEELN+ I + QY IQHESIPL+EEKQIL+EI+ LEGTR+KVIANAAMRAK++
Sbjct: 181 GPAICSSEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIK 240

Query: 266 DSMVHKEAIQDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTE 325
           +SM  K+ IQ QVK+MG  L+GV+KE QA+ A+I  + E LKA  D+I  L+ EL +V+E
Sbjct: 241 ESMGQKDDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTVSE 300

Query: 326 KRGRTYESMQQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLW 385
           KR + Y ++  LR+ RDE N+ +YQ+R+++NKA+DLAA+K+I  LE LA  EVEKF+SLW
Sbjct: 301 KRDKAYSNIHDLRRQRDETNSEYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLW 360

Query: 386 NGDKAFRDDYEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAK 445
              K FR+DYEKRIL SLD RQ+SRDGR+RNPDE+P++AP E AP +   +   +V +AK
Sbjct: 361 CSKKNFREDYEKRILQSLDSRQLSRDGRMRNPDEKPLIAP-EAAPSKATPSETEVVPKAK 420

Query: 446 EEPKPVPSDTLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLM 505
            +P+P   + +SA K D  V     K    ++ ++ A+++   +  L K   +E  VD  
Sbjct: 421 AKPQP-KEEPVSAPKPDATVAQNTEKAKDAVKVKNVADDDDDEVYGLGKPQKEEKPVDAA 480

Query: 506 KLKEMKRVEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTAS 565
             KEM++ EEIAKAK AMERKKKL EKAAAKA++RAQKEAEKK K+ ++EKKA KK    
Sbjct: 481 TAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEKK-EQEKKAKKK---- 540

Query: 566 GFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVD 625
                        +   EE  E+ E E+++   +   P K  + KE  IR+R  + RG +
Sbjct: 541 --------TGGNTETETEEVPEASE-EEIEAPVQEEKPQKEKVFKEKPIRNR-TRGRGPE 600

Query: 626 SIPKVIRKRKKANNYWVWAAPAAAAALLVILLLLGYYY 658
           +IP+ I KRKK+ NYWV+AAPAA   LL  LL+LGYYY
Sbjct: 601 TIPRAILKRKKSTNYWVYAAPAALVVLL--LLVLGYYY 610

BLAST of CmaCh01G012090 vs. TAIR 10
Match: AT3G15340.1 (proton pump interactor 2 )

HSP 1 Score: 263.5 bits (672), Expect = 4.7e-70
Identity = 218/608 (35.86%), Postives = 326/608 (53.62%), Query Frame = 0

Query: 63  PEENAPIKFGSHE-------DEPVKEQADVSSASNVPKDAADEWPAPKQIHTFYFIRYRP 122
           PE N  + FGS++            E   V S  + P  A +E    ++  +FYF++   
Sbjct: 17  PEMNDLVLFGSNQSVSSCDVSTVTTEDGTVFSGDSSPGGATEEDFPEEKPFSFYFVKQPA 76

Query: 123 YDDPNIKANIDLADKEIQKRSQARFQITETLKGKRAERAELITQVRALKDDNRQFKSIVE 182
           YDDP IKA ID A  EI + ++ R  ++   + ++AE   L  Q+++L   +  ++ ++E
Sbjct: 77  YDDPEIKAKIDEAGHEINRYNKDRIVVSNAQESEKAEILSLFGQMKSLVSKSEGYRVVIE 136

Query: 183 EKIKEIEPLNQALGKFRNANNSGRNGGLCSSEEELNASIQALQYHIQHESIPLSEEKQIL 242
           EK  E + L+++L   R + +      LC S+EEL+  I    Y I+H SI   EE  +L
Sbjct: 137 EKKMEFDALHESLRNLRCSTSD----QLCFSKEELDHLIYIAHYQIEHGSIGFEEEDWVL 196

Query: 243 REIKQLEGTREKVIANAAMRAKLQDSMVHKEAIQDQVKIMGGDLNGVRKEHQAVRAKIKH 302
           +E ++ +G               +DS+  KEA  ++VK M  +LN V+KE  A+  KI H
Sbjct: 197 KETEKADGI-----------VLSEDSLAEKEASINRVKSMAVELNEVKKELDAITWKINH 256

Query: 303 IEEALKAIDDDIKTLQEELTSVTEKRGRTYESMQQLRKNRDEGNAHFYQSRSIMNKAKDL 362
           + + +    ++++ L  +   + E+R R+YE ++ LR  RD+G A FYQS  +M KA++L
Sbjct: 257 LSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMRKAREL 316

Query: 363 AAKKDIKALEELACDEVEKFMSLWNGDKAFRDDYEKRILASLDVRQMSRDGRIRNPDERP 422
           AA  +++ LE  A  E ++FM+ WN DKAFRDDY KRI  SL  RQ+++DGRI++P+ + 
Sbjct: 317 AASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRIKDPEVQV 376

Query: 423 ILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSDTLSAQKVDKEVKPKAGKPSR-PLEKED 482
           +    E   P           R           ++   K  KE + KA   +R   E+ D
Sbjct: 377 VW---EKKVPVKGGEKVHETNREDSSSNSSQYGSVITDKRKKETRKKAMDFNRSSAEESD 436

Query: 483 KAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVEEIAKAKLAMERKKKLQEKAAAKASLR 542
             + E    EK  KE   E EVD   LKE +R E++ KA+LAMERK+KLQEKAAAKA++R
Sbjct: 437 VTDLEFSVYEKPKKE---EEEVDEETLKEREREEQLEKARLAMERKRKLQEKAAAKAAIR 496

Query: 543 AQKEAEKKLKEIDREKKAMKKMTASGFAFLPDEESAQADAVEEESKESKELEKVDENAEA 602
           AQKEAEKKLKE   EKKA KK  A+         S+     +   + +K+LEKV   A  
Sbjct: 497 AQKEAEKKLKEC--EKKAKKKAAAN---------SSSPSESDHSQEVTKDLEKVRTLA-- 556

Query: 603 PVPAKTNIQKET---------RIRSRGNKQRGVDSIPKVIRKRKKANNYWVWAAPAAAAA 654
            V  K   QKE          R + RG   RG +++PK I  R+KA+ YWVW   +AA A
Sbjct: 557 -VSGKEKHQKERSLFPKQRSFRYKHRG---RGTEALPKAILNRRKAHKYWVWGLSSAALA 586

BLAST of CmaCh01G012090 vs. TAIR 10
Match: AT5G36780.1 (AT5G36780 and AT5G36690 represent identical copies. The duplication within clone F27C7 is believed an artifact with only one true copy existing. )

HSP 1 Score: 235.0 bits (598), Expect = 1.8e-61
Identity = 216/634 (34.07%), Postives = 330/634 (52.05%), Query Frame = 0

Query: 32  GFEMVEGP-VDSVSL--ADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADVSSA 91
           GFE+V  P ++ + L   D S++ S+   D                E++ +    D SS 
Sbjct: 10  GFEVVPSPEINDLILYECDQSLTNSEVTTD---------------EEEDTIFSGGDSSSG 69

Query: 92  SNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITETLKG 151
               +D++ + P      +FY ++   YD+P IKA ID A++EI + ++ R  +    K 
Sbjct: 70  LAAEEDSSGDKPL-----SFYIVKQPVYDNPEIKAKIDEANQEIFRCNELRINVLSAKKS 129

Query: 152 KRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCSSEE 211
           + AE + L TQ+ +L   +  ++ ++EEK KE + L +AL   R   +      LC ++E
Sbjct: 130 ELAEVSSLYTQMESLVPQSEGYRMVIEEKKKEFDTLLEALRNLRCTTSD----QLCFTKE 189

Query: 212 ELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAI 271
           EL+      QY I++ SI L EE  +L+E ++ +G                  +  KEA 
Sbjct: 190 ELDHLSYIAQYQIEYGSIGLEEEDWMLKETEKPDGI----------------ILSEKEAS 249

Query: 272 QDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTYESM 331
            ++VK M  +LN V+ E  A+  KI  + + L    ++I+ L  E   + EKR R Y  +
Sbjct: 250 INRVKSMALELNEVKNELDAITWKINDLSDKLWKSQNNIRVLDLEKAHILEKRDRFYARI 309

Query: 332 QQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDD 391
           + LR  RD+GNA F+QS S+M KAK+LAA  +++ LE  A  EV++FM+LWN DKAFR+D
Sbjct: 310 KMLRIQRDKGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDRFMTLWNDDKAFRED 369

Query: 392 YEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSD 451
           Y +RI  SL  R+++ DGRI++ D         L     +  P   +KR+++  K    D
Sbjct: 370 YVRRISHSLCERELNEDGRIKDAD---------LQIFWEKKVPVKTIKRSEKVHKMNRED 429

Query: 452 TLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVE 511
           + S    D  V     K      KE K++  +   EK  K   KE E+D   LKE KR E
Sbjct: 430 SSSNSSEDGNVITDKRK------KETKSDVIV--YEKPKK---KEEEIDEEALKERKREE 489

Query: 512 EIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEI---------DREKKAMKKMTAS 571
           ++ KA+L MERK+KLQEKAAAKA++RAQKEAEKKLK I         + EKKA KK  A+
Sbjct: 490 QLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFFNECEKKAKKKAAAN 549

Query: 572 GFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVD 631
             +    ++    + V   +   KE  +  E    P       Q+  R + RG+   G +
Sbjct: 550 STSPSESDQVINDEKVRTLAVSGKEKHQ-KERLLFPK------QRSFRYKHRGS---GTE 573

Query: 632 SIPKVIRKRKKANNYWVWAAPAAAAALLVILLLL 654
           ++PK I  R+KA  YWVW   +AA A+ ++L++L
Sbjct: 610 ALPKAILNRRKARRYWVWGLSSAALAVSLVLVVL 573

BLAST of CmaCh01G012090 vs. TAIR 10
Match: AT5G36690.1 (AT5G36690 and AT5G36780 represent identical copies. The duplication within clone F27C7 is believed an artifact with only one true copy existing. )

HSP 1 Score: 235.0 bits (598), Expect = 1.8e-61
Identity = 216/634 (34.07%), Postives = 330/634 (52.05%), Query Frame = 0

Query: 32  GFEMVEGP-VDSVSL--ADNSVSKSKENGDLDQLPEENAPIKFGSHEDEPVKEQADVSSA 91
           GFE+V  P ++ + L   D S++ S+   D                E++ +    D SS 
Sbjct: 10  GFEVVPSPEINDLILYECDQSLTNSEVTTD---------------EEEDTIFSGGDSSSG 69

Query: 92  SNVPKDAADEWPAPKQIHTFYFIRYRPYDDPNIKANIDLADKEIQKRSQARFQITETLKG 151
               +D++ + P      +FY ++   YD+P IKA ID A++EI + ++ R  +    K 
Sbjct: 70  LAAEEDSSGDKPL-----SFYIVKQPVYDNPEIKAKIDEANQEIFRCNELRINVLSAKKS 129

Query: 152 KRAERAELITQVRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCSSEE 211
           + AE + L TQ+ +L   +  ++ ++EEK KE + L +AL   R   +      LC ++E
Sbjct: 130 ELAEVSSLYTQMESLVPQSEGYRMVIEEKKKEFDTLLEALRNLRCTTSD----QLCFTKE 189

Query: 212 ELNASIQALQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAI 271
           EL+      QY I++ SI L EE  +L+E ++ +G                  +  KEA 
Sbjct: 190 ELDHLSYIAQYQIEYGSIGLEEEDWMLKETEKPDGI----------------ILSEKEAS 249

Query: 272 QDQVKIMGGDLNGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTYESM 331
            ++VK M  +LN V+ E  A+  KI  + + L    ++I+ L  E   + EKR R Y  +
Sbjct: 250 INRVKSMALELNEVKNELDAITWKINDLSDKLWKSQNNIRVLDLEKAHILEKRDRFYARI 309

Query: 332 QQLRKNRDEGNAHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDD 391
           + LR  RD+GNA F+QS S+M KAK+LAA  +++ LE  A  EV++FM+LWN DKAFR+D
Sbjct: 310 KMLRIQRDKGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDRFMTLWNDDKAFRED 369

Query: 392 YEKRILASLDVRQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSD 451
           Y +RI  SL  R+++ DGRI++ D         L     +  P   +KR+++  K    D
Sbjct: 370 YVRRISHSLCERELNEDGRIKDAD---------LQIFWEKKVPVKTIKRSEKVHKMNRED 429

Query: 452 TLSAQKVDKEVKPKAGKPSRPLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVE 511
           + S    D  V     K      KE K++  +   EK  K   KE E+D   LKE KR E
Sbjct: 430 SSSNSSEDGNVITDKRK------KETKSDVIV--YEKPKK---KEEEIDEEALKERKREE 489

Query: 512 EIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEI---------DREKKAMKKMTAS 571
           ++ KA+L MERK+KLQEKAAAKA++RAQKEAEKKLK I         + EKKA KK  A+
Sbjct: 490 QLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFFNECEKKAKKKAAAN 549

Query: 572 GFAFLPDEESAQADAVEEESKESKELEKVDENAEAPVPAKTNIQKETRIRSRGNKQRGVD 631
             +    ++    + V   +   KE  +  E    P       Q+  R + RG+   G +
Sbjct: 550 STSPSESDQVINDEKVRTLAVSGKEKHQ-KERLLFPK------QRSFRYKHRGS---GTE 573

Query: 632 SIPKVIRKRKKANNYWVWAAPAAAAALLVILLLL 654
           ++PK I  R+KA  YWVW   +AA A+ ++L++L
Sbjct: 610 ALPKAILNRRKARRYWVWGLSSAALAVSLVLVVL 573

BLAST of CmaCh01G012090 vs. TAIR 10
Match: AT3G15340.2 (proton pump interactor 2 )

HSP 1 Score: 223.0 bits (567), Expect = 7.0e-58
Identity = 186/504 (36.90%), Postives = 276/504 (54.76%), Query Frame = 0

Query: 160 VRALKDDNRQFKSIVEEKIKEIEPLNQALGKFRNANNSGRNGGLCSSEEELNASIQALQY 219
           +++L   +  ++ ++EEK  E + L+++L   R + +      LC S+EEL+  I    Y
Sbjct: 1   MKSLVSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSD----QLCFSKEELDHLIYIAHY 60

Query: 220 HIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHKEAIQDQVKIMGGDL 279
            I+H SI   EE  +L+E ++ +G               +DS+  KEA  ++VK M  +L
Sbjct: 61  QIEHGSIGFEEEDWVLKETEKADGI-----------VLSEDSLAEKEASINRVKSMAVEL 120

Query: 280 NGVRKEHQAVRAKIKHIEEALKAIDDDIKTLQEELTSVTEKRGRTYESMQQLRKNRDEGN 339
           N V+KE  A+  KI H+ + +    ++++ L  +   + E+R R+YE ++ LR  RD+G 
Sbjct: 121 NEVKKELDAITWKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGK 180

Query: 340 AHFYQSRSIMNKAKDLAAKKDIKALEELACDEVEKFMSLWNGDKAFRDDYEKRILASLDV 399
           A FYQS  +M KA++LAA  +++ LE  A  E ++FM+ WN DKAFRDDY KRI  SL  
Sbjct: 181 AAFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCE 240

Query: 400 RQMSRDGRIRNPDERPILAPVELAPPRTEAAPKPIVKRAKEEPKPVPSDTLSAQKVDKEV 459
           RQ+++DGRI++P+ + +    E   P           R           ++   K  KE 
Sbjct: 241 RQLNQDGRIKDPEVQVVW---EKKVPVKGGEKVHETNREDSSSNSSQYGSVITDKRKKET 300

Query: 460 KPKAGKPSR-PLEKEDKAEEEIPGLEKLSKEIPKEPEVDLMKLKEMKRVEEIAKAKLAME 519
           + KA   +R   E+ D  + E    EK  KE   E EVD   LKE +R E++ KA+LAME
Sbjct: 301 RKKAMDFNRSSAEESDVTDLEFSVYEKPKKE---EEEVDEETLKEREREEQLEKARLAME 360

Query: 520 RKKKLQEKAAAKASLRAQKEAEKKLKEIDREKKAMKKMTASGFAFLPDEESAQADAVEEE 579
           RK+KLQEKAAAKA++RAQKEAEKKLKE   EKKA KK  A+         S+     +  
Sbjct: 361 RKRKLQEKAAAKAAIRAQKEAEKKLKEC--EKKAKKKAAAN---------SSSPSESDHS 420

Query: 580 SKESKELEKVDENAEAPVPAKTNIQKET---------RIRSRGNKQRGVDSIPKVIRKRK 639
            + +K+LEKV   A   V  K   QKE          R + RG   RG +++PK I  R+
Sbjct: 421 QEVTKDLEKVRTLA---VSGKEKHQKERSLFPKQRSFRYKHRG---RGTEALPKAILNRR 466

Query: 640 KANNYWVWAAPAAAAALLVILLLL 654
           KA+ YWVW   +AA A+ + L++L
Sbjct: 481 KAHKYWVWGLSSAALAVALFLVVL 466