CmUC08G144420 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC08G144420
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	Peptide chain release factor 1
Location	CmU531Chr08: 3839900 .. 3842170 (-)
RNA-Seq Expression	CmUC08G144420
Synteny	CmUC08G144420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTATTTAAATCATTTCCAGAGCTAAGAGGAGAAATTGGTGTTGTGAAGTACGAAAATGGCGAGCGCAAATATGCTCTGGAATTCCGCTAAGATTCTTGGATTTCCTGTTCCGTTAGCAAGCAACGGTCGATGCGCAAGGTTTGGTTGTACATTTCGGCATCATCCATTCGAGGGCTCTTTCTTCTTCAATTTGTTGCGTTCTAATTGCAGCTCTAGTACTGCAAATTCAGCATGGAATGGCAAAGAGTACTTAGCTTTGTCCGACGAAGAGCTGATGAGACAGTGCGAAATGGACACGTTCAAGGCGTCAGGTCCCGGTGGTCAGCACCGGAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCACTGCTCAGGTTTATCTTTGTTTCATACATATAATCAATTTATTAATATACTTCTGTTTCTTTTGCTCTGAAGTTAAATTCTTTGGCTCTTTATTGCCAAGGCTGTCGAGGATCGATCGCAGCATAAGAATCGAGCGAGTGCCTTAGCTCGTCTACGTGCTCTCTTGGCCCTAAAAGGTGCGGTGGATTATTAAATGCTTGATTTTTGTATCTCATTACCCAAAATGTATGTTCTCCTGCTGAATCATATATGATATGTGTTGCTGTGTTGAGCTCAGTCAGGAACCTTGTGGATCTTGAAGATTATTCCCCTCCTCCAGAGCTCCTTCAAATTCTACCTCTTAAGTCCACTATAAGGCCACCTGAGTGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGTATCTCTTTTTTCTAGTTCTTTCTATATGTCTATGTGTACATATGTGTGTGCGTTTTTTATTTGATTTTCTTGATATATGATAAGATTGTGAAGGTGATAGCCGAATTACCCACTTTGCAGGGGATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTAGCAGACACAGCTAAGCTTTTAGGGTATGTATGCTATTCATGTGTTACCCTTTGTTTCTTTTTACTTTATTTCCAACAGATTTAAGCATCCACAGTTAACGTAGAGAATGGGAGTGTTTCAATTCTTTGAGAAGATTGGGCTTTTAGCAATAAATTGAGTGTGCTGATGTGCGGATGTTAATGGTGGATAGGAAGACTAGTGGAAGGGGTATCATTGCTAGGTTTATGGTTTTTGTAGGAATGAACAAGAATGGCAATTTTGTCAAGGGCATGAGGGAAGCCAGTTTGGACCTCTGGAAGATGTTGGAGTAGAATTTTATTGGGAAGTGTCTAGCTGGATGCAAAAGCCTTGCCGGATCACTACGAGGAGGAAGGAGCTTTTCCATTTCTTGTATTACTTCTAAGGATGGTATCACTTTCGTTAAGAAGAGTAGGCTTAAGGACTTGATAAGGCACCTTACTACGGAAATAGACCAAATTTGGTTAGAGAGAGCTTCTTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGATATTCGTAAGTGTCTGGGCTAGCTTACGCGCACCTCGACTAATCTCACCGGACAACCCACCTAACCCTACAACATTTGGGTGTCAAGAAAACTCATAGGAAATTAATTCCTAGGTAGGTGGTCACCATGGATTGAACCCTTAACCTCTTAGTTATTTTTTGAGACTATGTCTCCTTTTTTCCACTAGGCCAACCCATGATGGTTGAGAGAGCTTCTTAGTTTGATGACTAGTTTTGTGGAAGGATTTTTCTTTTGATGTTTTGGTTTGGTTAGGGACCTTCGTTCTTTCTGTTTTGGTTTCTTCGATTTTTGTTCTATAGGAAAGCAATAGAGGTTGTCTCTCTCTCTTGTCATTAGTTAGGGAGTTTAGTTTTTGGCTTGGGAGAATAATGTTCCTTCATTAATATATTAACCTAGTGATTTCCACTATATCTCAAAATGAGATAAACCTGTGACATTACTATTATAAATACTAAAGTAATAAAACATACATGAGACATTAAATATTGAGAGTTTGATGAATTGATGCATTTGGAACTACTGAATTACAATTTAGATAAATAATCCAGAAATGATTCTTCAGGTTCCCATTTTTAATATGTTAATGATAATTGATATAGATTAGTTGCATCCTCTAATAATTATTTTAATGTTAAAAAATTTAGGTTAACCACTGGTGCTCTTTCAAGGTTAATCCTTTCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTTTGA

mRNA sequence

GCTATTTAAATCATTTCCAGAGCTAAGAGGAGAAATTGGTGTTGTGAAGTACGAAAATGGCGAGCGCAAATATGCTCTGGAATTCCGCTAAGATTCTTGGATTTCCTGTTCCGTTAGCAAGCAACGGTCGATGCGCAAGGTTTGGTTGTACATTTCGGCATCATCCATTCGAGGGCTCTTTCTTCTTCAATTTGTTGCGTTCTAATTGCAGCTCTAGTACTGCAAATTCAGCATGGAATGGCAAAGAGTACTTAGCTTTGTCCGACGAAGAGCTGATGAGACAGTGCGAAATGGACACGTTCAAGGCGTCAGGTCCCGGTGGTCAGCACCGGAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCACTGCTCAGGCTGTCGAGGATCGATCGCAGCATAAGAATCGAGCGAGTGCCTTAGCTCGTCTACGTGCTCTCTTGGCCCTAAAAGTCAGGAACCTTGTGGATCTTGAAGATTATTCCCCTCCTCCAGAGCTCCTTCAAATTCTACCTCTTAAGTCCACTATAAGGCCACCTGAGTGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGTGATAGCCGAATTACCCACTTTGCAGGGGATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTAGCAGACACAGCTAAGCTTTTAGGGAATGAACAAGAATGGCAATTTTGTCAAGGGCATGAGGGAAGCCAGTTTGGACCTCTGGAAGATGTTGGAGTAGAATTTTATTGGGAAGTGTCTAGCTGGATGCAAAAGCCTTGCCGGATCACTACGAGGAGGAAGGAGCTTTTCCATTTCTTGTTAACCACTGGTGCTCTTTCAAGGTTAATCCTTTCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTTTGA

Coding sequence (CDS)

ATGGCGAGCGCAAATATGCTCTGGAATTCCGCTAAGATTCTTGGATTTCCTGTTCCGTTAGCAAGCAACGGTCGATGCGCAAGGTTTGGTTGTACATTTCGGCATCATCCATTCGAGGGCTCTTTCTTCTTCAATTTGTTGCGTTCTAATTGCAGCTCTAGTACTGCAAATTCAGCATGGAATGGCAAAGAGTACTTAGCTTTGTCCGACGAAGAGCTGATGAGACAGTGCGAAATGGACACGTTCAAGGCGTCAGGTCCCGGTGGTCAGCACCGGAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCACTGCTCAGGCTGTCGAGGATCGATCGCAGCATAAGAATCGAGCGAGTGCCTTAGCTCGTCTACGTGCTCTCTTGGCCCTAAAAGTCAGGAACCTTGTGGATCTTGAAGATTATTCCCCTCCTCCAGAGCTCCTTCAAATTCTACCTCTTAAGTCCACTATAAGGCCACCTGAGTGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGTGATAGCCGAATTACCCACTTTGCAGGGGATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTAGCAGACACAGCTAAGCTTTTAGGGAATGAACAAGAATGGCAATTTTGTCAAGGGCATGAGGGAAGCCAGTTTGGACCTCTGGAAGATGTTGGAGTAGAATTTTATTGGGAAGTGTCTAGCTGGATGCAAAAGCCTTGCCGGATCACTACGAGGAGGAAGGAGCTTTTCCATTTCTTGTTAACCACTGGTGCTCTTTCAAGGTTAATCCTTTCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTTTGA

Protein sequence

MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAWNGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLPVIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFYWEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSKVTPYYF

Homology

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BLAST of CmUC08G144420 vs. NCBI nr
Match: XP_038891024.1 (peptide chain release factor 1 [Benincasa hispida])

HSP 1 Score: 392.5 bits (1007), Expect = 3.2e-105
Identity = 213/291 (73.20%), Postives = 220/291 (75.60%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAW 60
           MA+ANML NSAKILGFP+P+AS GR ARFGCTF HHPFEGSFFFN L SNCSS+TANS W
Sbjct: 1   MANANMLCNSAKILGFPLPIASKGRWARFGCTFWHHPFEGSFFFNSLHSNCSSTTANSVW 60

Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
           NGKEYL LSDEELM+QCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK
Sbjct: 61  NGKEYLVLSDEELMKQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120

Query: 121 NRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLP 180
           NRASALARLRA LALKVRN VDLEDYSPPPELLQILPLKSTIRPPECGPQIGP NSKFLP
Sbjct: 121 NRASALARLRAFLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPKNSKFLP 180

Query: 181 VIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFY 240
                    GMQALLDLISTLDGSV+DTAKLLG                           
Sbjct: 181 ---------GMQALLDLISTLDGSVSDTAKLLG--------------------------- 231

Query: 241 WEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                   LTTGALSRLILSDDALRMAVNDIR+SK
Sbjct: 241 ------------------------LTTGALSRLILSDDALRMAVNDIRISK 231

[Close]

BLAST of CmUC08G144420 vs. NCBI nr
Match: XP_023523846.1 (uncharacterized protein LOC111787964 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.4 bits (960), Expect = 9.1e-100
Identity = 204/291 (70.10%), Postives = 213/291 (73.20%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAW 60
           MA+ANMLW SAKIL  PVP+ASNGR  R GCTFR H FEGSFF N LRSNCSSS+ANS W
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRIHQFEGSFFSNSLRSNCSSSSANSVW 60

Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
            GKEYL LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK
Sbjct: 61  KGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120

Query: 121 NRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLP 180
           NRASALARLRA LALKVRN VDLEDYSPPPELLQILPLKSTIRPP CGPQIGPNNSKFLP
Sbjct: 121 NRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKFLP 180

Query: 181 VIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFY 240
                    GMQALLDLI TLDGS++DTAK +G                           
Sbjct: 181 ---------GMQALLDLIYTLDGSISDTAKYIG--------------------------- 231

Query: 241 WEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                   LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ------------------------LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of CmUC08G144420 vs. NCBI nr
Match: KAG6607552.1 (hypothetical protein SDJN03_00894, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037191.1 prfB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 373.6 bits (958), Expect = 1.6e-99
Identity = 204/291 (70.10%), Postives = 213/291 (73.20%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAW 60
           MA+ANMLW SAKIL  PVP+ASNGR  R GCTFR H FEGSFF N LRSNCSSS+ANS W
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRIHQFEGSFFSNSLRSNCSSSSANSIW 60

Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
            GKEYL LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK
Sbjct: 61  KGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120

Query: 121 NRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLP 180
           NRASALARLRA LALKVRN VDLEDYSPPPELLQILPLKSTIRPP CGPQIGPNNSKFLP
Sbjct: 121 NRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKFLP 180

Query: 181 VIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFY 240
                    GMQALLDLI TLDGS++DTAK +G                           
Sbjct: 181 ---------GMQALLDLIYTLDGSISDTAKYIG--------------------------- 231

Query: 241 WEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                   LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ------------------------LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of CmUC08G144420 vs. NCBI nr
Match: XP_022932469.1 (uncharacterized protein LOC111438876 [Cucurbita moschata])

HSP 1 Score: 371.7 bits (953), Expect = 5.9e-99
Identity = 203/291 (69.76%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAW 60
           MA+ANMLW SAKIL  PVP+ASNGR  R GCTFR H FEGSFF N LRS CSSS+ANS W
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRIHQFEGSFFSNSLRSTCSSSSANSVW 60

Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
            GKEYL LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK
Sbjct: 61  KGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120

Query: 121 NRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLP 180
           NRASALARLRA LALKVRN VDLEDYSPPPELLQILPLKSTIRPP CGPQIGPNNSKFLP
Sbjct: 121 NRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKFLP 180

Query: 181 VIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFY 240
                    GMQALLDLI TLDGS++DTAK +G                           
Sbjct: 181 ---------GMQALLDLIYTLDGSISDTAKYIG--------------------------- 231

Query: 241 WEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                   LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ------------------------LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of CmUC08G144420 vs. NCBI nr
Match: XP_008459491.1 (PREDICTED: uncharacterized protein LOC103498609 isoform X1 [Cucumis melo] >KAA0039363.1 peptide chain release factor 1 [Cucumis melo var. makuwa] >TYK00546.1 peptide chain release factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 370.9 bits (951), Expect = 1.0e-98
Identity = 209/294 (71.09%), Postives = 216/294 (73.47%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFG--CTFRHHPFEGS-FFFNLLRSNCSSSTAN 60
           MA+ANMLWNSAKILGFP P+ASN R ARFG    FRHHPFEGS FFFN LR NCSSS AN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SAWNGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           S  +GKEYL LSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SALARLRALLALKVRN VDLEDYSPPPELLQILP KST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPVIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGV 240
           FLP         GMQALLDLISTLDGSV+DTAKLLG                        
Sbjct: 181 FLP---------GMQALLDLISTLDGSVSDTAKLLG------------------------ 234

Query: 241 EFYWEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                      LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ---------------------------LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of CmUC08G144420 vs. ExPASy Swiss-Prot
Match: A7ZE19 (Peptide chain release factor 2 OS=Campylobacter concisus (strain 13826) OX=360104 GN=prfB PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-12
Identity = 34/59 (57.63%), Postives = 45/59 (76.27%), Query Frame = 0


Query: 75  RQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALARLRALL 134
           +  ++DT++ASG GGQH NK ESA+R+ HIPTGI  Q   DRSQHKNRA+A+  L++ L
Sbjct: 236 KDLKIDTYRASGAGGQHVNKTESAIRITHIPTGIVVQCQNDRSQHKNRATAMKMLKSRL 294

[Close]

BLAST of CmUC08G144420 vs. ExPASy Swiss-Prot
Match: B1XYX1 (Peptide chain release factor 1 OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) OX=395495 GN=prfA PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.3e-12
Identity = 39/75 (52.00%), Postives = 54/75 (72.00%), Query Frame = 0


Query: 64  EYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRA 123
           E ++L+  EL     +DT++ASG GGQH NK +SAVR+ H+PTG+ A+  +DRSQH+N+A
Sbjct: 213 EEVSLNPAEL----RIDTYRASGAGGQHINKTDSAVRVTHLPTGLVAECQDDRSQHRNKA 272

Query: 124 SALARLRALLALKVR 139
            ALA L A L  +VR
Sbjct: 273 RALAVLAARLRDRVR 283

[Close]

BLAST of CmUC08G144420 vs. ExPASy Swiss-Prot
Match: A5WFG1 (Peptide chain release factor 1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN=prfA PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.4e-12
Identity = 38/68 (55.88%), Postives = 48/68 (70.59%), Query Frame = 0


Query: 79  MDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALARLRA-LLALKV 138
           MDTF++SG GGQH N  +SAVRL HIPTG+ A+  ++RSQHKNRA A+  L A +   KV
Sbjct: 227 MDTFRSSGAGGQHVNTTDSAVRLTHIPTGVVAECQQERSQHKNRAKAMQMLIARIQQAKV 286

Query: 139 RNLVDLED 146
           +  VD  D
Sbjct: 287 QEQVDTAD 294

[Close]

BLAST of CmUC08G144420 vs. ExPASy Swiss-Prot
Match: Q0AC04 (Peptide chain release factor 1 OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=prfA PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.7e-12
Identity = 33/58 (56.90%), Postives = 44/58 (75.86%), Query Frame = 0


Query: 76  QCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALARLRALL 134
           +  +DTF+ASG GGQH NK +SA+R+ H+PTG+  +   +RSQHKNRA ALA L+A L
Sbjct: 223 ELRVDTFRASGAGGQHVNKTDSAIRITHLPTGVVVECQSERSQHKNRAQALALLQARL 280

[Close]

BLAST of CmUC08G144420 vs. ExPASy Swiss-Prot
Match: B1M766 (Peptide chain release factor 1 OS=Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1) OX=426355 GN=prfA PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.7e-12
Identity = 37/65 (56.92%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 78  EMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALARLRALLALKV 137
           ++DT +A G GGQH NK ESA+R+ H+PTGI     E+RSQHKNRA A+A LRA L    
Sbjct: 224 KIDTMRAQGAGGQHVNKTESAIRITHLPTGIVVFVQEERSQHKNRARAMALLRARLYEAE 283

Query: 138 RNLVD 143
           RN  D
Sbjct: 284 RNAKD 288

[Close]

BLAST of CmUC08G144420 vs. ExPASy TrEMBL
Match: A0A6J1EX22 (uncharacterized protein LOC111438876 OS=Cucurbita moschata OX=3662 GN=LOC111438876 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.9e-99
Identity = 203/291 (69.76%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAW 60
           MA+ANMLW SAKIL  PVP+ASNGR  R GCTFR H FEGSFF N LRS CSSS+ANS W
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRIHQFEGSFFSNSLRSTCSSSSANSVW 60

Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
            GKEYL LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK
Sbjct: 61  KGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120

Query: 121 NRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLP 180
           NRASALARLRA LALKVRN VDLEDYSPPPELLQILPLKSTIRPP CGPQIGPNNSKFLP
Sbjct: 121 NRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKFLP 180

Query: 181 VIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFY 240
                    GMQALLDLI TLDGS++DTAK +G                           
Sbjct: 181 ---------GMQALLDLIYTLDGSISDTAKYIG--------------------------- 231

Query: 241 WEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                   LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ------------------------LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of CmUC08G144420 vs. ExPASy TrEMBL
Match: A0A5A7T8S5 (Peptide chain release factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001580 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 4.9e-99
Identity = 209/294 (71.09%), Postives = 216/294 (73.47%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFG--CTFRHHPFEGS-FFFNLLRSNCSSSTAN 60
           MA+ANMLWNSAKILGFP P+ASN R ARFG    FRHHPFEGS FFFN LR NCSSS AN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SAWNGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           S  +GKEYL LSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SALARLRALLALKVRN VDLEDYSPPPELLQILP KST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPVIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGV 240
           FLP         GMQALLDLISTLDGSV+DTAKLLG                        
Sbjct: 181 FLP---------GMQALLDLISTLDGSVSDTAKLLG------------------------ 234

Query: 241 EFYWEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                      LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ---------------------------LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of CmUC08G144420 vs. ExPASy TrEMBL
Match: A0A1S3CAA2 (uncharacterized protein LOC103498609 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498609 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 4.9e-99
Identity = 209/294 (71.09%), Postives = 216/294 (73.47%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFG--CTFRHHPFEGS-FFFNLLRSNCSSSTAN 60
           MA+ANMLWNSAKILGFP P+ASN R ARFG    FRHHPFEGS FFFN LR NCSSS AN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SAWNGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           S  +GKEYL LSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SALARLRALLALKVRN VDLEDYSPPPELLQILP KST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPVIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGV 240
           FLP         GMQALLDLISTLDGSV+DTAKLLG                        
Sbjct: 181 FLP---------GMQALLDLISTLDGSVSDTAKLLG------------------------ 234

Query: 241 EFYWEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                      LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ---------------------------LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of CmUC08G144420 vs. ExPASy TrEMBL
Match: A0A0A0KUM8 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G646680 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 3.2e-98
Identity = 208/294 (70.75%), Postives = 216/294 (73.47%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTF--RHHPFEGS-FFFNLLRSNCSSSTAN 60
           MA+ANMLW+SAKILGFP P+ASN R ARF  TF  RH PFEGS FFFN LR NCSSS AN
Sbjct: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SAWNGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           S  NGK+YL LSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SALARLRALLALKVRN VDLEDYSPPPELLQILPLKST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPVIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGV 240
           FLP         GMQALLDLISTLDGSV+DTAKLLG                        
Sbjct: 181 FLP---------GMQALLDLISTLDGSVSDTAKLLG------------------------ 234

Query: 241 EFYWEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                      LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ---------------------------LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of CmUC08G144420 vs. ExPASy TrEMBL
Match: A0A6J1I8P1 (uncharacterized protein LOC111471993 OS=Cucurbita maxima OX=3661 GN=LOC111471993 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.6e-97
Identity = 200/291 (68.73%), Postives = 211/291 (72.51%), Query Frame = 0


Query: 1   MASANMLWNSAKILGFPVPLASNGRCARFGCTFRHHPFEGSFFFNLLRSNCSSSTANSAW 60
           MA+ NMLW SAKIL  PVP+ASNGR  R GCTFR H FEGSFF N LRSNCSSS+A S W
Sbjct: 1   MATTNMLWKSAKILSIPVPIASNGRWGRIGCTFRIHQFEGSFFSNSLRSNCSSSSAISVW 60

Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
            GKEYL LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK
Sbjct: 61  KGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120

Query: 121 NRASALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLP 180
           NRASALARLRA LALKVRN V+LEDYSPPPELLQILPLKSTIRPP CGPQIGPNNSKFLP
Sbjct: 121 NRASALARLRAQLALKVRNPVNLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKFLP 180

Query: 181 VIAELPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFY 240
                    GMQ+LLDLI TLDGS++DTAK +G                           
Sbjct: 181 ---------GMQSLLDLIYTLDGSISDTAKYIG--------------------------- 231

Query: 241 WEVSSWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                                   LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ------------------------LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of CmUC08G144420 vs. TAIR 10
Match: AT1G33330.1 (Class I peptide chain release factor )

HSP 1 Score: 219.2 bits (557), Expect = 4.6e-57
Identity = 127/227 (55.95%), Postives = 144/227 (63.44%), Query Frame = 0


Query: 65  YLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRAS 124
           YL  +DEELM+QC ++TF+ SGPGGQHRNKR+SAVRLKH+PTGI AQAVEDRSQHKNRAS
Sbjct: 86  YLKFTDEELMKQCRLETFRVSGPGGQHRNKRDSAVRLKHLPTGIVAQAVEDRSQHKNRAS 145

Query: 125 ALARLRALLALKVRNLVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLPVIAE 184
           AL RLR LLA+KVRN VD+E Y+PPPELLQILP KSTIR    G QIGPNN KF+P    
Sbjct: 146 ALNRLRTLLAIKVRNKVDIEAYAPPPELLQILPPKSTIR-TSSGSQIGPNNPKFVP---- 205

Query: 185 LPTLQGMQALLDLISTLDGSVADTAKLLGNEQEWQFCQGHEGSQFGPLEDVGVEFYWEVS 244
                GMQALLD+IS  DGS+AD+AKLLG                               
Sbjct: 206 -----GMQALLDVISASDGSIADSAKLLG------------------------------- 251

Query: 245 SWMQKPCRITTRRKELFHFLLTTGALSRLILSDDALRMAVNDIRMSK 292
                               L+TG LSRLILS D LRMAVN +R +K
Sbjct: 266 --------------------LSTGGLSRLILSHDGLRMAVNSMRAAK 251

[Close]

BLAST of CmUC08G144420 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 65.9 bits (159), Expect = 6.4e-11
Identity = 33/73 (45.21%), Postives = 49/73 (67.12%), Query Frame = 0


Query: 61  NGKEYLALSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 120
           +G   + ++D +L     ++ F++ G GGQH N  +SAVR+ HIPTGITA    +RSQH 
Sbjct: 336 DGSTRVEINDSDL----RIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHS 395

Query: 121 NRASALARLRALL 134
           N+ASA+A L++ L
Sbjct: 396 NKASAMAVLQSRL 404

[Close]

BLAST of CmUC08G144420 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 58.9 bits (141), Expect = 7.9e-09
Identity = 29/65 (44.62%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 79  MDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASAL----ARLRALLA 138
           +DT+++ G GGQH N   SAVR+ H+PTG+     ++RSQH NRA AL    ARL  +  
Sbjct: 276 IDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCARLYEIER 335

Query: 139 LKVRN 140
           L++++
Sbjct: 336 LRIQS 340

[Close]

BLAST of CmUC08G144420 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-08
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 0


Query: 52  SSSTANSAWNGKEYLALSDEELM------RQCEMDTFKASGPGGQHRNKRESAVRLKHIP 111
           S     ++++G E + L  EE +         ++   +A G GGQ+ NK E+AVR+ HIP
Sbjct: 288 SKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIP 347

Query: 112 TGITAQAVEDRSQHKNRASALARLRALL 134
           TG+  +  E+RSQ  N+  AL RL+A L
Sbjct: 348 TGVAVRCTEERSQLANKTRALIRLKAKL 375

[Close]

BLAST of CmUC08G144420 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-08
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 0


Query: 52  SSSTANSAWNGKEYLALSDEELM------RQCEMDTFKASGPGGQHRNKRESAVRLKHIP 111
           S     ++++G E + L  EE +         ++   +A G GGQ+ NK E+AVR+ HIP
Sbjct: 287 SKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIP 346

Query: 112 TGITAQAVEDRSQHKNRASALARLRALL 134
           TG+  +  E+RSQ  N+  AL RL+A L
Sbjct: 347 TGVAVRCTEERSQLANKTRALIRLKAKL 374

The following BLAST results are available for this feature:

BLAST of CmUC08G144420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891024.1	3.2e-105	73.20	peptide chain release factor 1 [Benincasa hispida]	[more]
XP_023523846.1	9.1e-100	70.10	uncharacterized protein LOC111787964 [Cucurbita pepo subsp. pepo]	[more]
KAG6607552.1	1.6e-99	70.10	hypothetical protein SDJN03_00894, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022932469.1	5.9e-99	69.76	uncharacterized protein LOC111438876 [Cucurbita moschata]	[more]
XP_008459491.1	1.0e-98	71.09	PREDICTED: uncharacterized protein LOC103498609 isoform X1 [Cucumis melo] >KAA00...	[more]

BLAST of CmUC08G144420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7ZE19	2.6e-12	57.63	Peptide chain release factor 2 OS=Campylobacter concisus (strain 13826) OX=36010...	[more]
B1XYX1	3.3e-12	52.00	Peptide chain release factor 1 OS=Leptothrix cholodnii (strain ATCC 51168 / LMG ...	[more]
A5WFG1	4.4e-12	55.88	Peptide chain release factor 1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN...	[more]
Q0AC04	5.7e-12	56.90	Peptide chain release factor 1 OS=Alkalilimnicola ehrlichii (strain ATCC BAA-110...	[more]
B1M766	5.7e-12	56.92	Peptide chain release factor 1 OS=Methylobacterium radiotolerans (strain ATCC 27...	[more]

BLAST of CmUC08G144420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EX22	2.9e-99	69.76	uncharacterized protein LOC111438876 OS=Cucurbita moschata OX=3662 GN=LOC1114388...	[more]
A0A5A7T8S5	4.9e-99	71.09	Peptide chain release factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3CAA2	4.9e-99	71.09	uncharacterized protein LOC103498609 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0KUM8	3.2e-98	70.75	RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G646680 P...	[more]
A0A6J1I8P1	1.6e-97	68.73	uncharacterized protein LOC111471993 OS=Cucurbita maxima OX=3661 GN=LOC111471993...	[more]

BLAST of CmUC08G144420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G33330.1	4.6e-57	55.95	Class I peptide chain release factor	[more]
AT1G56350.1	6.4e-11	45.21	Peptide chain release factor 2	[more]
AT2G47020.1	7.9e-09	44.62	Peptide chain release factor 1	[more]
AT5G36170.1	1.3e-08	37.50	high chlorophyll fluorescent 109	[more]
AT5G36170.2	1.3e-08	37.50	high chlorophyll fluorescent 109	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 76..134 e-value: 5.5E-20 score: 71.5
None	No IPR available	GENE3D	3.30.160.20		coord: 45..139 e-value: 5.4E-26 score: 92.4
None	No IPR available	PANTHER	PTHR43804	LD18447P	coord: 46..293
None	No IPR available	PANTHER	PTHR43804:SF6	CLASS I PEPTIDE CHAIN RELEASE FACTOR	coord: 46..293
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 76..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC08G144420.1	CmUC08G144420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
molecular_function	GO:0003747	translation release factor activity