CmUC04G069560 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC04G069560
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	Serine proteinase inhibitor
Location	CmU531Chr04: 5586808 .. 5587582 (+)
RNA-Seq Expression	CmUC04G069560
Synteny	CmUC04G069560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAATTTCCTCCCAATACAAAGAGAAGTTCTCAGTAATTTAGCAAAAGGCATGGCTGATATTCCTTTTCCTGGTAAGCTTAATTCAAAACTTTTAATCGTTTCTCTAAACCTTTCCACAATATTTATAAATCGACATTTGTTTTTCTACAGAGTAAGAGTAGACAATCTTAATTATATTTTTGTAGTGTAAATGATTAATTGAACATAAAATGATCATAGTATTTATTCACTTTTGCTTTTGACGTCGAGAAAAAGATGTTGATTTTCAAAAGTTGTTTGAATTTAGTCCAATATTTAGAGATGGTTGTAAAATTAGTTATTGATTTGCTACACATTTATAAGTAGTTTGTACTAATTATTGAAAAATTATTACGTTGAACTAGGAAAATCATCGTGGCTAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCATATCATACAAAAAGAGAATCCTCATGTAAAAATTATTGATATCCTATTAGCCGGCAGTCTACTAACTATGGACTTGAGAGAGGACAAAGTTCAAATCTTTGTGAACATACGAAAAGTTGTAGTTGAGATACCAAGGATTGGCTAACAAGAACTTGATATACTTTGTAAGAATAAAGTATTATTATACCATTAATTATGTGTTTATGTAAAAACTTGGGAGCATCATATAGATGTATAATCAAGCCAATATAATTAATGAAATGGGATCTCAAGAAAATTAGTAAGGTCTATTGTTTGGAATTTGTGTAGATTTAATTTCAAGATTTTAGGCTAGTTGGTT

mRNA sequence

TAATTTCCTCCCAATACAAAGAGAAGTTCTCAGTAATTTAGCAAAAGGCATGGCTGATATTCCTTTTCCTGGAAAATCATCGTGGCTAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCATATCATACAAAAAGAGAATCCTCATGTAAAAATTATTGATATCCTATTAGCCGGCAGTCTACTAACTATGGACTTGAGAGAGGACAAAGTTCAAATCTTTGTGAACATACGAAAAGTTGTAGTTGAGATACCAAGGATTGGCTAACAAGAACTTGATATACTTTGTAAGAATAAAGTATTATTATACCATTAATTATGTGTTTATGTAAAAACTTGGGAGCATCATATAGATGTATAATCAAGCCAATATAATTAATGAAATGGGATCTCAAGAAAATTAGTAAGGTCTATTGTTTGGAATTTGTGTAGATTTAATTTCAAGATTTTAGGCTAGTTGGTT

Coding sequence (CDS)

ATGGCTGATATTCCTTTTCCTGGAAAATCATCGTGGCTAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCATATCATACAAAAAGAGAATCCTCATGTAAAAATTATTGATATCCTATTAGCCGGCAGTCTACTAACTATGGACTTGAGAGAGGACAAAGTTCAAATCTTTGTGAACATACGAAAAGTTGTAGTTGAGATACCAAGGATTGGCTAA

Protein sequence

MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVVEIPRIG

Homology

[Close]

BLAST of CmUC04G069560 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 84.3 bits (207), Expect = 4.6e-13
Identity = 40/72 (55.56%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MAD   PGK+ W ELVGI++ IA+HII+K+N +V+ ++ +LAGS +T DLR D+V+IFVN
Sbjct: 1  MADFCPPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVN 60

Query: 61 IRKVVVEIPRIG 73
          I+ ++V+IP IG
Sbjct: 61 IKGIIVQIPTIG 72

[Close]

BLAST of CmUC04G069560 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 84.3 bits (207), Expect = 4.6e-13
Identity = 40/72 (55.56%), Postives = 57/72 (79.17%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MADI  PGK+ W ELVG++A  AK+II+K+NP+V+ + +LLAGS  T D+R D+V +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IRKVVVEIPRIG 73
          I  +VVE+P++G
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of CmUC04G069560 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 81.3 bits (199), Expect = 3.9e-12
Identity = 40/72 (55.56%), Postives = 56/72 (77.78%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MADI  PGK+ W ELVGI+A  A++II+K+NP V+ + +LLAG+  T D+R D+V +FVN
Sbjct: 1  MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 61 IRKVVVEIPRIG 73
          I + VVEIP++G
Sbjct: 61 IEEKVVEIPKVG 72

[Close]

BLAST of CmUC04G069560 vs. NCBI nr
Match: KGN50838.1 (hypothetical protein Csa_004692 [Cucumis sativus])

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-10
Identity = 38/72 (52.78%), Postives = 53/72 (73.61%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MADI  P K+ W ELVGI+A IA++II+K+NP+V+ + +LLAG+  T D R D+V +F N
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IRKVVVEIPRIG 73
          I   VVE+P +G
Sbjct: 61 IDGRVVEMPTVG 72

[Close]

BLAST of CmUC04G069560 vs. NCBI nr
Match: MQL67652.1 (hypothetical protein [Streptomyces vinaceus])

HSP 1 Score: 74.7 bits (182), Expect = 3.6e-10
Identity = 34/70 (48.57%), Postives = 50/70 (71.43%), Query Frame = 0


Query: 3  DIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIR 62
          D  F GK SW ELVG+  ++AK  IQKENP +  + I+L G+ +T+D R D+V+IFVN  
Sbjct: 12 DDGFEGKQSWPELVGVPGKVAKETIQKENPKLTQVVIVLIGTPITLDYRFDRVRIFVNYL 71

Query: 63 KVVVEIPRIG 73
           +V+++PR+G
Sbjct: 72 GIVIQVPRVG 81

[Close]

BLAST of CmUC04G069560 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.4e-11
Identity = 32/64 (50.00%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 9   KSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVVEI 68
           K SW EL+G  A+ AK IIQKENP +  ++ LL GS  T DLR ++V++FVN+  +VV+ 
Sbjct: 56  KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115

Query: 69  PRIG 73
           P++G
Sbjct: 116 PKVG 119

[Close]

BLAST of CmUC04G069560 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 7.6e-11
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 8   GKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVVE 67
           GK  W EL+G+ A  AK II+KENP +  I ILL GS +T D R D+V++FVNI   VV+
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 68  IPRI 72
           IPR+
Sbjct: 107 IPRV 110

[Close]

BLAST of CmUC04G069560 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.8e-10
Identity = 34/66 (51.52%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 7  PGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVV 66
          PGKSSW  LVG+   +AK II+++NP+VK + IL  G+ +T D R ++V+I+VN R +VV
Sbjct: 4  PGKSSWPHLVGVGGSVAKAIIERQNPNVKAV-ILEEGTPVTKDFRCNRVRIWVNKRGLVV 63

Query: 67 EIPRIG 73
            PRIG
Sbjct: 64 SPPRIG 68

[Close]

BLAST of CmUC04G069560 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 8.4e-10
Identity = 31/65 (47.69%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 8   GKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVVE 67
           GK  W EL+G+  ++AK II+KEN  +  + ILL GS +T+D+R D+V++F NI   VV+
Sbjct: 43  GKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVD 102

Query: 68  IPRIG 73
           IP +G
Sbjct: 103 IPVVG 107

[Close]

BLAST of CmUC04G069560 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.1e-09
Identity = 30/62 (48.39%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 8   GKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVVE 67
           GK  W EL+G+  ++AK II+KENP +  I ILL+GS +T+D   D+V++F NI   VV+
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 68  IP 70
           +P
Sbjct: 107 MP 108

[Close]

BLAST of CmUC04G069560 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.2e-13
Identity = 40/72 (55.56%), Postives = 57/72 (79.17%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MADI  PGK+ W ELVG++A  AK+II+K+NP+V+ + +LLAGS  T D+R D+V +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IRKVVVEIPRIG 73
          I  +VVE+P++G
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of CmUC04G069560 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-12
Identity = 40/72 (55.56%), Postives = 56/72 (77.78%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MADI  PGK+ W ELVGI+A  A++II+K+NP V+ + +LLAG+  T D+R D+V +FVN
Sbjct: 1  MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 61 IRKVVVEIPRIG 73
          I + VVEIP++G
Sbjct: 61 IEEKVVEIPKVG 72

[Close]

BLAST of CmUC04G069560 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.8e-11
Identity = 38/72 (52.78%), Postives = 53/72 (73.61%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVN 60
          MADI  P K+ W ELVGI+A IA++II+K+NP+V+ + +LLAG+  T D R D+V +F N
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IRKVVVEIPRIG 73
          I   VVE+P +G
Sbjct: 61 IDGRVVEMPTVG 72

[Close]

BLAST of CmUC04G069560 vs. ExPASy TrEMBL
Match: A0A6J1HFK9 (inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LOC111463862 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.0e-10
Identity = 41/74 (55.41%), Postives = 51/74 (68.92%), Query Frame = 0


Query: 1  MADIPFPGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSL--LTMDLREDKVQIF 60
          MAD   PGK+ W ELVGIEA IA+ IIQKENP+V I+ I L GS   +TM+    +V++ 
Sbjct: 1  MADFCPPGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLL 60

Query: 61 VNIRKVVVEIPRIG 73
          VNI  V+V IP IG
Sbjct: 61 VNINGVIVGIPSIG 74

[Close]

BLAST of CmUC04G069560 vs. ExPASy TrEMBL
Match: A0A6N2AU12 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.3e-09
Identity = 36/64 (56.25%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 8   GKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVVE 67
           GK  W EL+G+ A  AK II+KENP +  I ILL GS +T D R D+V++FVNI  VVV+
Sbjct: 40  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGVVVQ 99

Query: 68  IPRI 72
           IPR+
Sbjct: 100 IPRV 103

[Close]

BLAST of CmUC04G069560 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.8e-08
Identity = 29/66 (43.94%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 7  PGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVV 66
          PGKSSW EL+G + E AK +I++ENP +K + I+L G+++       +V ++VN   +VV
Sbjct: 35 PGKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIVV 94

Query: 67 EIPRIG 73
          +IP IG
Sbjct: 95 QIPIIG 99

[Close]

BLAST of CmUC04G069560 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.7 bits (130), Expect = 3.6e-08
Identity = 27/66 (40.91%), Postives = 43/66 (65.15%), Query Frame = 0


Query: 7  PGKSSWLELVGIEAEIAKHIIQKENPHVKIIDILLAGSLLTMDLREDKVQIFVNIRKVVV 66
          P K+SW EL G   + A  +I++ENP V    ++L GS +T D R D+V++FV+  ++VV
Sbjct: 6  PRKNSWPELTGTNGDYAAVVIERENPTVNAA-VILDGSPVTADFRCDRVRVFVDGNRIVV 65

Query: 67 EIPRIG 73
          + P+ G
Sbjct: 66 KTPKSG 70

The following BLAST results are available for this feature:

BLAST of CmUC04G069560 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571494.1	4.6e-13	55.56	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN50840.1	4.6e-13	55.56	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
ALD47589.1	3.9e-12	55.56	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50838.1	1.6e-10	52.78	hypothetical protein Csa_004692 [Cucumis sativus]	[more]
MQL67652.1	3.6e-10	48.57	hypothetical protein [Streptomyces vinaceus]	[more]

BLAST of CmUC04G069560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P20076	4.4e-11	50.00	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
P16231	7.6e-11	54.69	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P19873	3.8e-10	51.52	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q00783	8.4e-10	47.69	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P05118	1.1e-09	48.39	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]

BLAST of CmUC04G069560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KME7	2.2e-13	55.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A0M3SAG8	1.9e-12	55.56	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KMM0	7.8e-11	52.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
A0A6J1HFK9	3.0e-10	55.41	inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6N2AU12	4.3e-09	56.25	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1	[more]

BLAST of CmUC04G069560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	2.8e-08	43.94	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	3.6e-08	40.91	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 11..24 score: 50.0 coord: 49..59 score: 41.11 coord: 37..48 score: 36.96
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..72 e-value: 1.4E-19 score: 70.5
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 5..72
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 5..72 e-value: 8.1E-21 score: 76.2
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 5..72
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 7..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC04G069560.1	CmUC04G069560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity