CmUC02G036350 (gene) Watermelon (USVL531) v1

Overview
NameCmUC02G036350
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionN-acyl-L-amino-acid amidohydrolase
LocationCmU531Chr02: 20667060 .. 20670577 (+)
RNA-Seq ExpressionCmUC02G036350
SyntenyCmUC02G036350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAAGCAGAATGGACCCTTGTCGTCTTCTCTAAAACTAAAAGGCAGCCTTCCTCTTCTCCTCAAATTTGTCAAGAGTCTCTACATTCCATCATTGTTGTGTATTGTACCAAAAACCTTCCGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACCTTTTTCTTGTTTCTCTCAATTTCTACTTCTGACAAAGACCCATCTTCTTCCTCTGCAATCATATCAAGGTTCCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCAAATACTATGAAGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTAGGACAATTGAATTCGTCGCGGGCAAGCCTCTGGTTCTCCTCAAATGGCCTGGTTCCAACCCTGATCTCCCCTCGATCCTCCTTAATTCGCATACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCCAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGAATCTGGGTTTGAGCCTCTGCGCTCTGTTTACCTATCTTTTGTTCCGGATGAAGAAATTGGTGGCCATGGCGGTGCTGAGAAATTTGCTGAATCTGATGAGTTCGAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGTTCTAAGCGAACCCCTTTCGCTGATTTCTGATGGGTGAGTTTCTTTCATCCTTGTGCTTCACATGTTTAGACTAATATTGTTCGAAAATCATCAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAGAAGAGCCCCTGGTGGTTGGTGATCAAGGCAGTTGGAGCGCCAGGTCATGGAGCTAAGCTTTATGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATTTGATCAAGTCTGGTTTGAAGACTGAAGGAGATGTTGTCTCTGTCAATATGGTCTTCTTAAAATCTGGCACTCCATCTCCTACTGTGAGTTGATCTTTGAAACTTTTTTCTCCATGAATGCTTGAAGTTCTAATTCCTCTGATCATCTAATAGATGAAACCATCACTCTGCATTTGAGTATTAGTTCCATCGCTGCATCTGGCAAATTAGCTAAGTTTATGAGTATTGTTCTATTCTTTGTTTGAGTTCCAAGTCGAACTTCAAAACCAATGGTTTAGACAGTTGAAGTACTATAAATTAGGCTATTATATCAATCAAGAATGTGTGACTTGAATTTTTGATGTTGTTGTTATCCTTACTGTCATAACAGACTATGTTGTTGATATTCTGTGTCATATTAAAGGATCTTTGTGCTAATGAAACTCTGTTGCCAATATAACCTGATGGCTTACTCTGTTTTCTACCAGGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGATGTTCGCCTCCCACCGACTGCAAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCTTCTCGTAATATGACGTTTGAGGTAACAATTTAGGATTTTTTTATTCTAGCCTAAGAAGCATGGAATACAATCACAACATGTTAATTTCTAAGGGTGTGTTTGGGCCAAGGAGTTGGGAATTAAGACTAGGGTAGTAGGAGCCTGTGAATTCCCCTCCTTGTTTGGCTCAATGAGTTGGTGGGTCCCACTACTAAAAAAACATCAATTTTATATCTTATCAACCGCTTACATCGTCGGCCCCAAGAGTTCACCACTCCCTAGACTTCATAACTCCTTACTCCTTTCTATTCCACTCCTTACCTTGGACACCCCTTAACCAACTAAGACACGATTAATGGACATATACTTGCATATATATGTTAGTATACCTAATTTTTAATGATTTTCATATTATAAATTGGTTTGTCTATGATTGAAATGATAAAATATGTTACGAATGTAGAACATCTATCTAACAAGTGGTGGAGTGATGGATGTCCAATACACAGCAGACTCAAACTAAAATGTCTGCTTAAAAGTTTCTAACCACTATCACTTATACAGAGTATGCTGATTATGAATTCCAAAATTCAATGGATTTTACAATGTAAATTCTTCCTCTCATATCTTTCCTTGTGCTTGATTCTTGCTCAGTTTAAGCAGAAGGAATCTATTTACAACAAGTTTGGGAAACCAGCTCTTACTGCTATTGACAAATCTAACCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCCAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCCCGATACTTTCGAAATCTTGGCCTTCCTGCAATTGGCTTCTCTCCAATGGCCAACACTCCTATTCTTCTTCATGACCATAATGAGGTGAGAATCTTAGTACTGTGTTCTTCTGTGCAGGCAGGAACTTTTAAGTTGGATGTAGCATAAATTTTGATCTTAGTTGAAATCTTGATTGATCATCTTCATTCTGAGTAACCGCCTGGTAGTAGATTGATGCTGAGCAGTTGACACTCTAGAAGCAATCATAATTGCTTCTCATGCTGTGGCAGAATCGAACTAAACCGTGCTCTATTCCTTCACCTCCATTTTAGATTTGGATATGGATCTAGTTGGTTGTTTATGTGCCTGACATTGAACTGCGTTGTTATCTAAGTCGTTAATATGTTCATCTGTTTCAGTTTCTGAACCAAGCTGAGTATTTGAAAGGCATTGAGGTGTACGAGTCAATAATCAAGGCTTATGCTTCTTATGACGATGAAAAATCGACCGAGAGCTTCAAAGATGAGTTATAAGAAAGCAGCGAAGCAGTGTTATCTATTCCAGCATTCAGCGACGAGGTTTGAATTTGCTGTCTGTAGGATTAGGGAAGTTTAGAGCATGGGATTGGATGGGAGGAAGGGATATGATTTTGTTCCATAATTGGGTCTTGTGGAACTACTTCATTGAACTTTGGTGGTGGTGCATTTATTTGCAAGTACCATATAATTCAGGCAGCAGAATTTTCTATCAAATAAAGCGAAAACAGAATTTGCTTTGGAATAGGTTCAAAAGCTTCTTCAGCAAGCTACTATTCCAGCAACAATTCCTTATTACTCATATCTCCTCTCGAACCTTATTGATGATGCCCCATCGGATCTTCTCACTATTCTCTTGGCAAGGACCGGTTTGTGGGAACTGACTTTTGAAAGAGAAAAAAAGGATATTTGTGCGTTATTTCTCTGTGAACTTGAATGAGAAAGATCAAAAGAGAGAGTGAGAAGGAATTATTTTCTCTCCTCTCACTCATCATTGAAAAATGAGAATATTGATCTTTTGATAAACATTAATAATAATGAGAGAGAGTTAAAGAGAATATTCTATTCAAGCAGATGATTAGAATATTAATAAACATTAATATATGATTAATGACATTACATATTTTGATTGCTG

mRNA sequence

ATGTCAAAGCAGAATGGACCCTTGTCGTCTTCTCTAAAACTAAAAGGCAGCCTTCCTCTTCTCCTCAAATTTGTCAAGAGTCTCTACATTCCATCATTGTTGTGTATTGTACCAAAAACCTTCCGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACCTTTTTCTTGTTTCTCTCAATTTCTACTTCTGACAAAGACCCATCTTCTTCCTCTGCAATCATATCAAGGTTCCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCAAATACTATGAAGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTAGGACAATTGAATTCGTCGCGGGCAAGCCTCTGGTTCTCCTCAAATGGCCTGGTTCCAACCCTGATCTCCCCTCGATCCTCCTTAATTCGCATACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCCAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGAATCTGGGTTTGAGCCTCTGCGCTCTGTTTACCTATCTTTTGTTCCGGATGAAGAAATTGGTGGCCATGGCGGTGCTGAGAAATTTGCTGAATCTGATGAGTTCGAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAGAAGAGCCCCTGGTGGTTGGTGATCAAGGCAGTTGGAGCGCCAGGTCATGGAGCTAAGCTTTATGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATTTGATCAAGTCTGGTTTGAAGACTGAAGGAGATGTTGTCTCTGTCAATATGGTCTTCTTAAAATCTGGCACTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGATGTTCGCCTCCCACCGACTGCAAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCTTCTCGTAATATGACGTTTGAGTTTAAGCAGAAGGAATCTATTTACAACAAGTTTGGGAAACCAGCTCTTACTGCTATTGACAAATCTAACCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCCAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCCCGATACTTTCGAAATCTTGGCCTTCCTGCAATTGGCTTCTCTCCAATGGCCAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTGAACCAAGCTGAGTATTTGAAAGGCATTGAGGTGTACGAGTCAATAATCAAGGCTTATGCTTCTTATGACGATGAAAAATCGACCGAGAGCTTCAAAGATGAGTTATAAGAAAGCAGCGAAGCAGTGTTATCTATTCCAGCATTCAGCGACGAGGTTTGAATTTGCTGTCTGTAGGATTAGGGAAGTTTAGAGCATGGGATTGGATGGGAGGAAGGGATATGATTTTGTTCCATAATTGGGTCTTGTGGAACTACTTCATTGAACTTTGGTGGTGGTGCATTTATTTGCAAGTACCATATAATTCAGGCAGCAGAATTTTCTATCAAATAAAGCGAAAACAGAATTTGCTTTGGAATAGGTTCAAAAGCTTCTTCAGCAAGCTACTATTCCAGCAACAATTCCTTATTACTCATATCTCCTCTCGAACCTTATTGATGATGCCCCATCGGATCTTCTCACTATTCTCTTGGCAAGGACCGGTTTGTGGGAACTGACTTTTGAAAGAGAAAAAAAGGATATTTGTGCGTTATTTCTCTGTGAACTTGAATGAGAAAGATCAAAAGAGAGAGTGAGAAGGAATTATTTTCTCTCCTCTCACTCATCATTGAAAAATGAGAATATTGATCTTTTGATAAACATTAATAATAATGAGAGAGAGTTAAAGAGAATATTCTATTCAAGCAGATGATTAGAATATTAATAAACATTAATATATGATTAATGACATTACATATTTTGATTGCTG

Coding sequence (CDS)

ATGTCAAAGCAGAATGGACCCTTGTCGTCTTCTCTAAAACTAAAAGGCAGCCTTCCTCTTCTCCTCAAATTTGTCAAGAGTCTCTACATTCCATCATTGTTGTGTATTGTACCAAAAACCTTCCGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACCTTTTTCTTGTTTCTCTCAATTTCTACTTCTGACAAAGACCCATCTTCTTCCTCTGCAATCATATCAAGGTTCCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCAAATACTATGAAGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTAGGACAATTGAATTCGTCGCGGGCAAGCCTCTGGTTCTCCTCAAATGGCCTGGTTCCAACCCTGATCTCCCCTCGATCCTCCTTAATTCGCATACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCCAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGAATCTGGGTTTGAGCCTCTGCGCTCTGTTTACCTATCTTTTGTTCCGGATGAAGAAATTGGTGGCCATGGCGGTGCTGAGAAATTTGCTGAATCTGATGAGTTCGAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAGAAGAGCCCCTGGTGGTTGGTGATCAAGGCAGTTGGAGCGCCAGGTCATGGAGCTAAGCTTTATGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATTTGATCAAGTCTGGTTTGAAGACTGAAGGAGATGTTGTCTCTGTCAATATGGTCTTCTTAAAATCTGGCACTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGATGTTCGCCTCCCACCGACTGCAAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCTTCTCGTAATATGACGTTTGAGTTTAAGCAGAAGGAATCTATTTACAACAAGTTTGGGAAACCAGCTCTTACTGCTATTGACAAATCTAACCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCCAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCCCGATACTTTCGAAATCTTGGCCTTCCTGCAATTGGCTTCTCTCCAATGGCCAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTGAACCAAGCTGAGTATTTGAAAGGCATTGAGGTGTACGAGTCAATAATCAAGGCTTATGCTTCTTATGACGATGAAAAATCGACCGAGAGCTTCAAAGATGAGTTATAA

Protein sequence

MSKQNGPLSSSLKLKGSLPLLLKFVKSLYIPSLLCIVPKTFRAMNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYASYDDEKSTESFKDEL
Homology
BLAST of CmUC02G036350 vs. NCBI nr
Match: KAA0047810.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 865.5 bits (2235), Expect = 2.2e-247
Identity = 423/448 (94.42%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS++DPSS SAI+SRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPL++LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of CmUC02G036350 vs. NCBI nr
Match: XP_008448158.1 (PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 864.0 bits (2231), Expect = 6.3e-247
Identity = 423/448 (94.42%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS+++PSS SAIISRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPL++LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of CmUC02G036350 vs. NCBI nr
Match: XP_038876663.1 (aminoacylase-1 [Benincasa hispida])

HSP 1 Score: 863.6 bits (2230), Expect = 8.2e-247
Identity = 421/448 (93.97%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           M FKSLHFLLS IT FL LS+STS++DPSS+SAIISRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MIFKSLHFLLSFITLFLLLSVSTSEEDPSSTSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAK+LSLES+TIEFV GKPL+LLKWPG NPDLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKALSLESQTIEFVEGKPLILLKWPGLNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGFEPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESD F KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDN ALEN
Sbjct: 181 AESDVFNKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNAALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIE++RRFRASQFDLIK+GLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESIRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKES+YNKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESVYNKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVY+SIIKAYASYD +K TESFKDEL
Sbjct: 421 GIEVYKSIIKAYASYDGDKPTESFKDEL 448

BLAST of CmUC02G036350 vs. NCBI nr
Match: XP_004139978.1 (aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cucumis sativus])

HSP 1 Score: 860.5 bits (2222), Expect = 6.9e-246
Identity = 423/448 (94.42%), Postives = 434/448 (96.88%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS++DPSS SAIISRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPLV+LKWPGSNP+LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVR+PPT NPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

BLAST of CmUC02G036350 vs. NCBI nr
Match: XP_022140673.1 (aminoacylase-1 [Momordica charantia])

HSP 1 Score: 820.1 bits (2117), Expect = 1.0e-233
Identity = 401/448 (89.51%), Postives = 422/448 (94.20%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MN K ++ LLS+IT FLFLS S +D++ SSSSAII+RFQQYLQINTVQP+P YYEAADFI
Sbjct: 1   MNLKYVNLLLSVITIFLFLSSSIADEESSSSSAIIARFQQYLQINTVQPNPHYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSL+LES+TIEFV GKPL+LLKWPGSNPDL SILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLALESQTIEFVEGKPLILLKWPGSNPDLSSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDS GNIYARGSQDMKCVGMQYLEAIRRLK  GFEP RSVYLSFVPDEEIGG  GAEKF
Sbjct: 121 HIDSLGNIYARGSQDMKCVGMQYLEAIRRLKALGFEPARSVYLSFVPDEEIGGRAGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNV IVLDEGLPSPGENYRVFYGE+SPWW+VIKAVGAPGHGAKLYDNTALEN
Sbjct: 181 AESDEFQKLNVGIVLDEGLPSPGENYRVFYGERSPWWMVIKAVGAPGHGAKLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIE+VRRFRASQFDL+K+GLK EG+VVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESVRRFRASQFDLVKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPTANPESLERRIAEEWAP SRNMTFEFKQKESI++KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESIFDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKL  PEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIE YESIIKAYAS+   K TESFKDEL
Sbjct: 421 GIEAYESIIKAYASFIAHKPTESFKDEL 448

BLAST of CmUC02G036350 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.4e-100
Identity = 185/411 (45.01%), Postives = 255/411 (62.04%), Query Frame = 0

Query: 69  KDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLSLESRTIEFVAGKPLVLL 128
           KDP S    ++ F+QYL+I TVQP+P Y  A  F+  +A+ L L  + IE V G  + +L
Sbjct: 4   KDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVL 63

Query: 129 KWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLE 188
            WPG+NP LPSILLNSHTDVVP     W H P  A  DS G IYARGSQDMK V +QYLE
Sbjct: 64  TWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLE 123

Query: 189 AIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGEN 248
           A+RRLK  G    R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +P + 
Sbjct: 124 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 183

Query: 249 YRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETVRRFRASQFDLIKSG--L 308
           + VFY E+SPWW+ + + G PGH ++  ++TA E L K I ++  FR  +   +++   L
Sbjct: 184 FTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHL 243

Query: 309 KTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAP 368
           K EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E+++      
Sbjct: 244 K-EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQE 303

Query: 369 ASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDA 428
           A   +TFEF Q      KF +P +T  D S+PWW     A +  N  L  PEIFPA+TD+
Sbjct: 304 AGEGVTFEFAQ------KFTEPRMTPTDDSDPWWAAFSGACKAMNLTL-EPEIFPAATDS 363

Query: 429 RYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 478
           RY R +G+PA+GFSPM  TP+LLHDHNE L++  +L+G+++Y  ++ A AS
Sbjct: 364 RYIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

BLAST of CmUC02G036350 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.8e-98
Identity = 180/411 (43.80%), Postives = 253/411 (61.56%), Query Frame = 0

Query: 69  KDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLSLESRTIEFVAGKPLVLL 128
           K P S    ++ F+QYL+I TVQP+P Y  A  F+  +A+ L L  + IE   G  + +L
Sbjct: 4   KGPESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVL 63

Query: 129 KWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLE 188
            WPG+NP L SILLNSHTDVVP     W H P  A  DS G IYARG+QDMK V +QYLE
Sbjct: 64  TWPGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLE 123

Query: 189 AIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGEN 248
           A+RRLK  G    R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +P + 
Sbjct: 124 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 183

Query: 249 YRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETVRRFRASQFDLIKSG--L 308
           + VFY E+SPWW+ + + G PGH ++  ++TA E L K + ++  FR  +   +++   L
Sbjct: 184 FTVFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHL 243

Query: 309 KTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAP 368
           K EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E+++      
Sbjct: 244 K-EGAVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQE 303

Query: 369 ASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDA 428
           A   +TFEF Q      KF +P +T  D ++PWW     A +  N  L  PEIFPA+TD+
Sbjct: 304 AGEGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDS 363

Query: 429 RYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 478
           RY R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 364 RYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of CmUC02G036350 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.1e-97
Identity = 177/413 (42.86%), Postives = 249/413 (60.29%), Query Frame = 0

Query: 67  SDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLSLESRTIEFVAGKPLV 126
           + K P      ++ F+QYL+I TVQP P Y  A  F    A+ L L  + +E   G  + 
Sbjct: 2   TSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVT 61

Query: 127 LLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQY 186
           +L WPG+NP L SILLNSHTDVVP     W+H P  A  DS G IY RG+QDMKCV +QY
Sbjct: 62  VLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQY 121

Query: 187 LEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPG 246
           LEA++RLK  G    R+++++FVPDEE+GGH G E F +  EF  L     LDEGL +P 
Sbjct: 122 LEAVKRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPT 181

Query: 247 ENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETVRRFRASQFDLIKSG- 306
           + + VFY E+SPWW+ + + G PGH ++  ++TA E L K + ++  FR  ++  ++S  
Sbjct: 182 DAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNP 241

Query: 307 -LKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEW 366
            LK EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E ++    
Sbjct: 242 HLK-EGSVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWC 301

Query: 367 APASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPAST 426
             A   +TFEF Q      KF +P +T  D ++PWW     A +     L  PEIFPA+T
Sbjct: 302 QEAGEGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAAT 361

Query: 427 DARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 478
           D+RY R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 362 DSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of CmUC02G036350 vs. ExPASy Swiss-Prot
Match: Q55DP8 (Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.1e-97
Identity = 180/402 (44.78%), Postives = 258/402 (64.18%), Query Frame = 0

Query: 78  ISRFQQYLQINTVQPSPKYYEAADFIISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDL 137
           ++ F+++L+I T  P+P Y  +  F++ +AK  ++         G P+VL+K  G  P+L
Sbjct: 10  VTVFREFLKIRTDHPTPDYESSTKFLVEKAKEYNIPYEVYR-ETGTPIVLMKIEGLEPNL 69

Query: 138 PSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESG 197
            ++LLNSH DVVPA H  W   P  A  D  GNI+ RG+QDMKCV MQ+LE  RR+ +SG
Sbjct: 70  KTVLLNSHVDVVPAVHDSWKVDPFSAWKDESGNIFGRGTQDMKCVCMQFLEVARRIVQSG 129

Query: 198 FEPLRSVYLSFVPDEEIGGHG-GAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEK 257
            +  R+++LSFVPDEEIGG G G EKF  +++F +LN+ + LDEGL SP  ++ VFYGE+
Sbjct: 130 QKLKRTLHLSFVPDEEIGGSGKGMEKFVYTEKFRQLNIGLCLDEGLASPTNDFTVFYGER 189

Query: 258 SPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETVRRFRASQFDLIKSGL----KTEGDV 317
           +PWW+ I AVG  GHG++  + TA+E L ++I  +  FR  QF+ +  G     K  GDV
Sbjct: 190 APWWVHITAVGNAGHGSRFIEGTAIEKLMRTINKMLAFRQEQFESLHHGQHECGKKLGDV 249

Query: 318 VSVNMVFLKSGTP--SPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPASRN 377
            S+N+  LK+G P      F  N+ P++AEAGFD+R+PPT N +    +I +EW  A   
Sbjct: 250 TSLNLTVLKAGIPIDHSNNFSYNVIPTQAEAGFDIRIPPTVNLDQFLDQI-KEWT-AEEG 309

Query: 378 MTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARYFR 437
           ++F+F       +   K  +T +D  N WW   +E+ +  +  L   EIFPA+TD+R+ R
Sbjct: 310 LSFKFA------SYIPKNEMTKLDSDNKWWENFKESCKKMDINLVT-EIFPAATDSRFIR 369

Query: 438 NLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESII 473
           NLG+PA GFSP+ NTPILLHDHNEFLN+  YL+GI+++  II
Sbjct: 370 NLGIPAFGFSPINNTPILLHDHNEFLNEKVYLRGIDIFMGII 401

BLAST of CmUC02G036350 vs. ExPASy Swiss-Prot
Match: Q03154 (Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.6e-96
Identity = 176/413 (42.62%), Postives = 247/413 (59.81%), Query Frame = 0

Query: 67  SDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLSLESRTIEFVAGKPLV 126
           + K P      ++ F+QYL+I TVQP P Y  A  F    A+ L L  + +E   G  + 
Sbjct: 2   TSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVT 61

Query: 127 LLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQY 186
           +L WPG+NP L SILLNSHTDVVP     W+H P  A  DS G IYARG+QDMKCV +QY
Sbjct: 62  VLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQY 121

Query: 187 LEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPG 246
           LEA+RRLK  G    R+++++FVPDEE+GGH G E F +  EF  L     LDEG+ +P 
Sbjct: 122 LEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPT 181

Query: 247 ENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETVRRFRASQFDLIKSG- 306
           + + VFY E+SPWW+ + + G PGH ++  ++TA E L K + ++  FR  ++  ++S  
Sbjct: 182 DAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNP 241

Query: 307 -LKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEW 366
            LK EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E ++    
Sbjct: 242 HLK-EGSVTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWC 301

Query: 367 APASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPAST 426
             A   +T EF Q      K+  P +T  D SNPWW       ++ N  L  PEI PA+T
Sbjct: 302 QAAGEGVTLEFAQ------KWMHPQVTPTDDSNPWWAAFSRVCKDMNLTL-EPEIMPAAT 361

Query: 427 DARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 478
           D RY R +G+PA+GFSPM  TP+LLHDH+E L++A +L+G+++Y  ++ A AS
Sbjct: 362 DNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

BLAST of CmUC02G036350 vs. ExPASy TrEMBL
Match: A0A5A7TXA6 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00610 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.0e-247
Identity = 423/448 (94.42%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS++DPSS SAI+SRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPL++LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of CmUC02G036350 vs. ExPASy TrEMBL
Match: A0A5D3BSI0 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00590 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.0e-247
Identity = 423/448 (94.42%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS+++PSS SAIISRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPL++LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of CmUC02G036350 vs. ExPASy TrEMBL
Match: A0A1S3BJ13 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.0e-247
Identity = 423/448 (94.42%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS+++PSS SAIISRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPL++LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of CmUC02G036350 vs. ExPASy TrEMBL
Match: A0A0A0KAV8 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.3e-246
Identity = 423/448 (94.42%), Postives = 434/448 (96.88%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MNFKSLHFLLSIIT F FLSISTS++DPSS SAIISRFQQYLQINTVQPSP+YYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSLSLES TIEFV GKPLV+LKWPGSNP+LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIETVRRFRASQFDLIK+GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVR+PPT NPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIEVYESIIKAYASYD +K  ESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

BLAST of CmUC02G036350 vs. ExPASy TrEMBL
Match: A0A6J1CII7 (N-acyl-L-amino-acid amidohydrolase OS=Momordica charantia OX=3673 GN=LOC111011275 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 5.0e-234
Identity = 401/448 (89.51%), Postives = 422/448 (94.20%), Query Frame = 0

Query: 44  MNFKSLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFI 103
           MN K ++ LLS+IT FLFLS S +D++ SSSSAII+RFQQYLQINTVQP+P YYEAADFI
Sbjct: 1   MNLKYVNLLLSVITIFLFLSSSIADEESSSSSAIIARFQQYLQINTVQPNPHYYEAADFI 60

Query: 104 ISQAKSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 163
           ISQAKSL+LES+TIEFV GKPL+LLKWPGSNPDL SILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLALESQTIEFVEGKPLILLKWPGSNPDLSSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 164 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKF 223
           HIDS GNIYARGSQDMKCVGMQYLEAIRRLK  GFEP RSVYLSFVPDEEIGG  GAEKF
Sbjct: 121 HIDSLGNIYARGSQDMKCVGMQYLEAIRRLKALGFEPARSVYLSFVPDEEIGGRAGAEKF 180

Query: 224 AESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALEN 283
           AESDEF+KLNV IVLDEGLPSPGENYRVFYGE+SPWW+VIKAVGAPGHGAKLYDNTALEN
Sbjct: 181 AESDEFQKLNVGIVLDEGLPSPGENYRVFYGERSPWWMVIKAVGAPGHGAKLYDNTALEN 240

Query: 284 LFKSIETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 343
           LFKSIE+VRRFRASQFDL+K+GLK EG+VVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESVRRFRASQFDLVKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 344 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 403
           DVRLPPTANPESLERRIAEEWAP SRNMTFEFKQKESI++KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESIFDKFGKPALTAIDKSNPWWNLL 360

Query: 404 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 463
           EEAVRNANGKL  PEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420

Query: 464 GIEVYESIIKAYASYDDEKSTESFKDEL 492
           GIE YESIIKAYAS+   K TESFKDEL
Sbjct: 421 GIEAYESIIKAYASFIAHKPTESFKDEL 448

BLAST of CmUC02G036350 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 649.0 bits (1673), Expect = 2.9e-186
Identity = 318/440 (72.27%), Postives = 369/440 (83.86%), Query Frame = 0

Query: 52  LLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLS 111
           LL ++   + L +S    D     AI+SRFQ+YL+INTVQP+P+YY+A DFIISQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 112 LESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNI 171
           LES+TIEFV GKPL+LLKW GS+P LP+ LLNSHTDVVP E  KWTH PL AH+D HG+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 172 YARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEK 231
           YARGSQDMKCVGMQYLEAIR+L+ SGF+PLRSVYLSFVPDEEIGGH GAEKFAES  F+ 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 232 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETV 291
           LN+AIVLDEGLPSP E+YRVFYGE+SPWWLVIKA G PGHGAKLYDN+A+ENL KSIE++
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 292 RRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTA 351
           RRFRASQFDL+K+G   EGDVVSVNM FLK+GTPSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 352 NPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNAN 411
           + E+LERR+ EEWAPA+RNM+FEFKQK +     GK  LTA D SNPWW LLE AV+ A 
Sbjct: 303 DAEALERRLVEEWAPAARNMSFEFKQKLT-----GKQFLTAADDSNPWWGLLENAVKEAG 362

Query: 412 GKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESI 471
           G+ + PEIFPASTDARYFR  G+PA GFSP++NTP LLHDHNE+L +AEYLKGIEVY SI
Sbjct: 363 GRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSI 422

Query: 472 IKAYASYDDEKSTESFKDEL 492
           IKAYASY+ +  +   +DEL
Sbjct: 423 IKAYASYESKSGS---RDEL 430

BLAST of CmUC02G036350 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-184
Identity = 318/443 (71.78%), Postives = 369/443 (83.30%), Query Frame = 0

Query: 52  LLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLS 111
           LL ++   + L +S    D     AI+SRFQ+YL+INTVQP+P+YY+A DFIISQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 112 LESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNI 171
           LES+TIEFV GKPL+LLKW GS+P LP+ LLNSHTDVVP E  KWTH PL AH+D HG+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 172 YARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEK 231
           YARGSQDMKCVGMQYLEAIR+L+ SGF+PLRSVYLSFVPDEEIGGH GAEKFAES  F+ 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 232 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETV 291
           LN+AIVLDEGLPSP E+YRVFYGE+SPWWLVIKA G PGHGAKLYDN+A+ENL KSIE++
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 292 RRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTA 351
           RRFRASQFDL+K+G   EGDVVSVNM FLK+GTPSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 352 NPESLERRIAEEWAPASRNMTFE---FKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVR 411
           + E+LERR+ EEWAPA+RNM+FE   FKQK +     GK  LTA D SNPWW LLE AV+
Sbjct: 303 DAEALERRLVEEWAPAARNMSFELGQFKQKLT-----GKQFLTAADDSNPWWGLLENAVK 362

Query: 412 NANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVY 471
            A G+ + PEIFPASTDARYFR  G+PA GFSP++NTP LLHDHNE+L +AEYLKGIEVY
Sbjct: 363 EAGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVY 422

Query: 472 ESIIKAYASYDDEKSTESFKDEL 492
            SIIKAYASY+ +  +   +DEL
Sbjct: 423 VSIIKAYASYESKSGS---RDEL 433

BLAST of CmUC02G036350 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.9e-149
Identity = 238/432 (55.09%), Postives = 323/432 (74.77%), Query Frame = 0

Query: 52  LLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQAKSLS 111
           L ++I F +  S+ +S    S     I+RFQQYL+ NT  P+P Y     F+I+QA+S+ 
Sbjct: 7   LWTLIIFSIIFSLQSSS---SEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIG 66

Query: 112 LESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNI 171
           L ++TIEF++GKP++L+ W GSNP+LPSIL NSH D VPAE  KWT+PP  AH    G+I
Sbjct: 67  LTTKTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHI 126

Query: 172 YARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDEFEK 231
           YARG+QD KC+G+QYLE+IR LK  GF PLR++++S+VP+EEIGG  G  KFA S EF+ 
Sbjct: 127 YARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKD 186

Query: 232 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIETV 291
           LN+   +DEG  +PG+ +RVFY ++ PW  VIKA G PGHGAKLYDN+A+ENL KS+E +
Sbjct: 187 LNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELI 246

Query: 292 RRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTA 351
            RFR SQFD +K+G     +V+SVN V+LK+GTP+ TGFVMN+QPSEAEAG+D+RLPP A
Sbjct: 247 SRFRESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMA 306

Query: 352 NPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNAN 411
           +P+ +++RIAEEWAP+ RNMT+  ++K  + +  G+P +T ++ SNPWW++ ++AV    
Sbjct: 307 DPDVMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMG 366

Query: 412 GKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESI 471
           GKLA PEI  ++TD+R+ R LG+P  GFSPM NTPILLHDHNEFL    ++KGIEVYES+
Sbjct: 367 GKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESV 426

Query: 472 IKAYASYDDEKS 484
           I A +S++ E +
Sbjct: 427 ISALSSFEGESA 435

BLAST of CmUC02G036350 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 518.5 bits (1334), Expect = 6.0e-147
Identity = 239/432 (55.32%), Postives = 314/432 (72.69%), Query Frame = 0

Query: 48  SLHFLLSIITFFLFLSISTSDKDPSSSSAIISRFQQYLQINTVQPSPKYYEAADFIISQA 107
           ++  LL  +  F F+    S          I+RFQQYL+ NT  P+P Y     F++ QA
Sbjct: 2   AVSLLLWTLFLFSFIFSLQSHSKEEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQA 61

Query: 108 KSLSLESRTIEFVAGKPLVLLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDS 167
           +S+ L SRTIEFV GKP++LL W GSN +L SIL NSH D VPAE  KW HPP  AH   
Sbjct: 62  RSIGLTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTI 121

Query: 168 HGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFVPDEEIGGHGGAEKFAESD 227
            G+IYARG+QD KC+G+QYLEAIR LK   F PLR++++S+VP+EEIGG GG  KFA S 
Sbjct: 122 DGHIYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASS 181

Query: 228 EFEKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNTALENLFKS 287
           EF+ LN+  V+DEG  SPG+ +RVFY E++PW L I+A G PGHGAKLYDN+A+ENL KS
Sbjct: 182 EFKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKS 241

Query: 288 IETVRRFRASQFDLIKSGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRL 347
           +E + RFR SQFD +K+G     +V+SVN V+LK+GTPS TGFVMN+QPSEAE G+D+RL
Sbjct: 242 VELISRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRL 301

Query: 348 PPTANPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAV 407
           PP A+P +L++RIAEEWAP+ RNMT+  KQ+  + +  G+P +T  + +NPWW++ ++AV
Sbjct: 302 PPMADPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAV 361

Query: 408 RNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEV 467
               GKLA PEI  ++TD+R+ R LG+P +GFSPM NTPIL+HDHNEFL    ++KGI V
Sbjct: 362 EATGGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGV 421

Query: 468 YESIIKAYASYD 480
           YES+I A +S++
Sbjct: 422 YESVISALSSFE 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0047810.12.2e-24794.42aminoacylase-1 [Cucumis melo var. makuwa][more]
XP_008448158.16.3e-24794.42PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis mel... [more]
XP_038876663.18.2e-24793.97aminoacylase-1 [Benincasa hispida][more]
XP_004139978.16.9e-24694.42aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cu... [more]
XP_022140673.11.0e-23389.51aminoacylase-1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q99JW21.4e-10045.01Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1[more]
Q6AYS73.8e-9843.80Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1[more]
Q6PTT01.1e-9742.86Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1[more]
Q55DP81.1e-9744.78Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1[more]
Q031543.6e-9642.62Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TXA61.0e-24794.42N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BSI03.0e-24794.42N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BJ133.0e-24794.42N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 ... [more]
A0A0A0KAV83.3e-24694.42N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE... [more]
A0A6J1CII75.0e-23489.51N-acyl-L-amino-acid amidohydrolase OS=Momordica charantia OX=3673 GN=LOC11101127... [more]
Match NameE-valueIdentityDescription
AT4G38220.12.9e-18672.27Peptidase M20/M25/M40 family protein [more]
AT4G38220.21.2e-18471.78Peptidase M20/M25/M40 family protein [more]
AT1G44820.12.9e-14955.09Peptidase M20/M25/M40 family protein [more]
AT1G44180.16.0e-14755.32Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.360coord: 260..370
e-value: 3.4E-8
score: 35.3
NoneNo IPR availableGENE3D1.10.150.900coord: 397..477
e-value: 9.5E-31
score: 107.4
NoneNo IPR availablePIRSFPIRSF036696ACY-1coord: 67..480
e-value: 2.6E-175
score: 579.9
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 68..259
e-value: 1.8E-59
score: 202.6
NoneNo IPR availablePANTHERPTHR45892:SF4BNAA06G40880D PROTEINcoord: 62..482
NoneNo IPR availablePANTHERPTHR45892AMINOACYLASE-1coord: 62..482
NoneNo IPR availableCDDcd05646M20_AcylaseI_likecoord: 74..476
e-value: 0.0
score: 667.438
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 75..476
IPR010159N-acyl-L-amino-acid amidohydrolaseTIGRFAMTIGR01880TIGR01880coord: 72..477
e-value: 6.9E-188
score: 622.2
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 141..473
e-value: 3.2E-37
score: 128.2
IPR001261ArgE/DapE/ACY1/CPG2/YscS, conserved sitePROSITEPS00759ARGE_DAPE_CPG2_2coord: 176..215
IPR001261ArgE/DapE/ACY1/CPG2/YscS, conserved sitePROSITEPS00758ARGE_DAPE_CPG2_1coord: 140..149

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC02G036350.1CmUC02G036350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004046 aminoacylase activity
molecular_function GO:0016787 hydrolase activity