Homology
BLAST of Clc10G04860 vs. NCBI nr
Match:
XP_038904656.1 (DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1614.7 bits (4180), Expect = 0.0e+00
Identity = 843/939 (89.78%), Postives = 860/939 (91.59%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGR GSD+C
Sbjct: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
S+NGQSSNNQKRMT LPKTSPMRSEIGS KASKS I+EP VKPLGT+LKVSDSM SR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
Query: 121 LVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDSD 180
LVN AVK+EN +I T +NSDSEDDKPLSIRLKGNS+QPNKG VPT PKNKVKK SGDSD
Sbjct: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLPS 240
DEIPLSSKFQMKS SG LG KQHGFDEKKPLVP+VHQNGSTSRDKLQKPA+LSSKRPLP+
Sbjct: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
Query: 241 ADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEIDD-DDDIPISQRMKKISAPVNKSS 300
ADSH+ SQIS KKPKLSDSSTPLSSK VSLKAKQE+DD DDDIPISQRMKK+SAPVNKSS
Sbjct: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480
Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGR 540
VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540
Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
GEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQK
Sbjct: 541 GEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQK 600
Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660
EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660
Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
DKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
KAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
GDV EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KELKIDLDRAK
Sbjct: 781 GDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAK 840
Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 889
Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Clc10G04860 vs. NCBI nr
Match:
XP_008449103.1 (PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DNA topoisomerase 1-like [Cucumis melo])
HSP 1 Score: 1585.1 bits (4103), Expect = 0.0e+00
Identity = 831/940 (88.40%), Postives = 854/940 (90.85%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR GSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 HLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDS 180
+LVNAAVKEEN +INP+ +NSDSEDDKPLSIRLKGNSNQPNKG V T PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLP 240
DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPA+LSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 SADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEI-DDDDDIPISQRMKKISAPVNKS 300
+ DSHS SQ S KKPKLSDSS+PLSSK VSLKAKQE+ DDDDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480
Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRG 540
VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD TRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889
Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Clc10G04860 vs. NCBI nr
Match:
XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1575.8 bits (4079), Expect = 0.0e+00
Identity = 823/939 (87.65%), Postives = 853/939 (90.84%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRPGSD C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
S+NGQSSN QKRMT LPKTSPMR EIGS KASKSSIQEPLVK LGTTLKVSDSM SRPK
Sbjct: 61 SANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDSD 180
L NAAVKEEN +I+PT DNSDSEDDKPLSIRLKGNSN P+KGP+PT KNK+KK S DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLPS 240
DE+PLSSKFQMKSASG LGG+QHGFDEKKPL KVHQNGSTSRDKLQKPA++SSKRPLP+
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEI-DDDDDIPISQRMKKISAPVNKSS 300
ADSHSSSQIS KKPKLSDSSTPLS+ VSLKAKQE+ DDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480
Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGR 540
VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540
Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600
Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660
EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660
Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAK 840
Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887
Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
BLAST of Clc10G04860 vs. NCBI nr
Match:
KAG7015549.1 (DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1573.9 bits (4074), Expect = 0.0e+00
Identity = 821/939 (87.43%), Postives = 854/939 (90.95%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRPGSD C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
S+NGQSSN QKRMT LPKTSPMR EIGS KA+KSSIQEPLVK LGTTLKVSDSM SRPK
Sbjct: 61 SANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDSD 180
L NAA+KEEN +I+PT +NSDSEDDKPLSIRLKGNSN P+KGP+PT KNK+KK S DSD
Sbjct: 121 LANAALKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLPS 240
DE+PLSSKFQMKSASG LGGKQHGFDEKKPL KVHQNGSTSRDKLQKPA++SSKRPLP+
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQE-IDDDDDIPISQRMKKISAPVNKSS 300
ADSHSSSQIS+KKPKLSDSSTPLS+ VSLKAKQE +DDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480
Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGR 540
VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540
Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600
Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660
EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660
Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887
Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP+FRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPEFRF 887
BLAST of Clc10G04860 vs. NCBI nr
Match:
KAA0065816.1 (DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomerase 1-like [Cucumis melo var. makuwa])
HSP 1 Score: 1573.9 bits (4074), Expect = 0.0e+00
Identity = 831/958 (86.74%), Postives = 854/958 (89.14%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR GSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 HLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDS 180
+LVNAAVKEEN +INP+ +NSDSEDDKPLSIRLKGNSNQPNKG V T PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLP 240
DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPA+LSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 SADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEI-DDDDDIPISQRMKKISAPVNKS 300
+ DSHS SQ S KKPKLSDSS+PLSSK VSLKAKQE+ DDDDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480
Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRG 540
VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD TRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
Query: 901 DPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMDVDPDFRF 939
DPRITVAWCKRHEVPIEK IFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMDVDPDFRF 907
BLAST of Clc10G04860 vs. ExPASy Swiss-Prot
Match:
P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)
HSP 1 Score: 1003.0 bits (2592), Expect = 2.2e-291
Identity = 577/992 (58.17%), Postives = 683/992 (68.85%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
S V S NG TP KTS ++S + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGN----------SNQP---NKGP 180
K ++ E DSEDD+PLS L GN S QP N G
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPTAPKNKVKKPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSR 240
P +++ K +SDDE P+SS F+ K SG GG Q DEKKPLV K+HQNGST +
Sbjct: 181 RPLDRASRIIK--DESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPAILSSKRPLPSADSHSSSQISMKKPKLSDSSTPLSSKPVS-------------- 300
+++ +L KRPL + S+ Q S+KK K+S S T + K S
Sbjct: 241 NEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300
Query: 301 --LKAKQ--------EIDDDDDIPISQRMKKISAPVNKSSSSKQTVS------------- 360
+KAKQ + DDDDD+PIS+R K S+ N SS+ + V
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360
Query: 361 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 420
VVS + K +KK K SKY S+K PS+GDGQKKWTTLVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420
Query: 421 ILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
ILYKGKPVDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480
Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFW 540
FTPIY+W+ EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEK------------ 540
Query: 541 KNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMG 600
+GNFRVEPPGLFRGRGEHPKMG
Sbjct: 541 --------------------------------------IGNFRVEPPGLFRGRGEHPKMG 600
Query: 601 KLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFL 660
KLK+RI P +IT+NIGKGAPIPECPI GE WKEV+HDNTVTWLAFW DPIN KEFKYVFL
Sbjct: 601 KLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFL 660
Query: 661 AASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRA 720
A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVATYLIDKLALRA
Sbjct: 661 GAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRA 720
Query: 721 GNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQ 780
GNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ
Sbjct: 721 GNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQ 780
Query: 781 SGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKI 840
+GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KI
Sbjct: 781 AGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKI 840
Query: 841 VTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLK 900
V YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 841 VVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLE 900
Query: 901 DADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAW 939
+DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAW
Sbjct: 901 GSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAW 913
BLAST of Clc10G04860 vs. ExPASy Swiss-Prot
Match:
Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)
HSP 1 Score: 994.2 bits (2569), Expect = 1.0e-288
Identity = 570/991 (57.52%), Postives = 687/991 (69.32%), Query Frame = 0
Query: 1 MAVETFGKAPMFD-----DDDDEDNMPIVFKRSS-----ASRQNQLDSEIKHSQSQRLNK 60
MA E F K + + +D+DED++P+VFKR+S +R + +++ +++S +
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIG--ST 60
Query: 61 LSGRPGSDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKV 120
S P +S +S+++ R + + P S + +S+++ PL K
Sbjct: 61 KSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSV-----QRSTLKSPLRDDRSVVAKE 120
Query: 121 SDSMGSRPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNK-------GP 180
+ G P +++ +DD DSEDDKPLS RLK +S + K G
Sbjct: 121 RNGFGKAP-------------SVSKSDD-EDSEDDKPLSARLKLDSKEVTKQPSSSGRGS 180
Query: 181 VPTAPKNKVKKPSGDS--------DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKV 240
A + +P G S D+ P+SS Q K++ G K ++K+PLV +
Sbjct: 181 TQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNI 240
Query: 241 HQNG---STSRDKLQKPAILSSKRPLPSADSHSSSQISMKKPKLS--------------- 300
+NG T Q PA KRPL SS+Q S+K+PKLS
Sbjct: 241 DRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHIS 300
Query: 301 ----------DSSTPLSSKPVSLKAKQEIDDDDDIPISQRMKKISAPVNKSSSSKQTVSK 360
D+S PL + ++K + D DD +PI+ RMK S+ NKSSS+K + SK
Sbjct: 301 ATQDAKGKNLDASKPLRANQATVK-EDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSSK 360
Query: 361 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 420
+++SS + +K K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVKI
Sbjct: 361 MIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVKI 420
Query: 421 LYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDF 480
L++GKPVDLTPEQEEVATMFAVM++T+Y KPKF+ENFWNDWRKLLGKNH+I++LDDCDF
Sbjct: 421 LFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDF 480
Query: 481 TPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWK 540
TPIY+WY EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E+
Sbjct: 481 TPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRER------------- 540
Query: 541 NLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGK 600
+GNFRVEPPGLFRGRGEHPKMGK
Sbjct: 541 -------------------------------------IGNFRVEPPGLFRGRGEHPKMGK 600
Query: 601 LKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLA 660
LK+RIRP DITINIGK APIPECPIPGE WKEV+HDNTVTWLAFW+DPIN KEFKYVFLA
Sbjct: 601 LKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLA 660
Query: 661 ASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRAG 720
ASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATYLIDKLALRAG
Sbjct: 661 ASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAG 720
Query: 721 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 780
NEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE V+KAI QFQ+
Sbjct: 721 NEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 780
Query: 781 GKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 840
GKS TDDLFD+LDT+KLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV EK+V
Sbjct: 781 GKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPEKVV 840
Query: 841 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKD 900
YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL IDLDRAKKG+ PL
Sbjct: 841 VYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTPLMG 900
Query: 901 ADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 939
+DGKRKRNLTPEALEKKI QT KIEK+ERDM+TKED+KTVALGTSKINY+DPRITVAWC
Sbjct: 901 SDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVAWC 911
BLAST of Clc10G04860 vs. ExPASy Swiss-Prot
Match:
P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)
HSP 1 Score: 812.4 bits (2097), Expect = 5.4e-234
Identity = 483/860 (56.16%), Postives = 580/860 (67.44%), Query Frame = 0
Query: 120 HLVNAAVKEENFTINPTDDNSDSEDDKPLSIR-----LKGNSNQPNKG-----PVP-TAP 179
+++ +KEE ++ DS+DDKPLS + L GNS+ G P P T+P
Sbjct: 16 NVMEKTLKEEG---QSRSNSEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSP 75
Query: 180 KNKVKKPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQK 239
K ++ K +DE+ LSS+ Q+ +GA + DE KPL +L+
Sbjct: 76 KPRIIK---GPEDEMLLSSELQLN--AGASNAESSDSDESKPLA-----------SELED 135
Query: 240 PAILSSKRPLPSADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEIDDDDDIPISQRM 299
P+ +KRPL +S KK KLS+ S P + K + ++E DDD IS R
Sbjct: 136 PSSSLNKRPL--IQPKNSDPSPSKKAKLSEPSAPTNRK-LKTAEQEEAAADDDPSISNRN 195
Query: 300 KKISAPVNKSSSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTT 359
KK + P +K S K+ VS+S+ K V SK +K +L +GQ
Sbjct: 196 KKSTTPASKVSDKKKRPD--VSASVNK--------VDFSKSLKVPLVLVKGRNGQ----P 255
Query: 360 LVHNGVIFPPPYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWND 419
LVHNGVIFPP Y+ HGVK+LY+ PVDLTPEQEEVATMFAVM +T+YM KPKF+ENF +D
Sbjct: 256 LVHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSD 315
Query: 420 WRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAI 479
WRK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE K + ++EKYMWA+
Sbjct: 316 WRKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAV 375
Query: 480 LDGVKEKVILIYHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGN 539
+DG+ + +VGN
Sbjct: 376 VDGMSRE-------------------------------------------------KVGN 435
Query: 540 FRVEPPGLFRGRGEHPKMGKLKRRIRPS-DITINIGKGAPIPECPIPGESWKEVRHDNTV 599
FRV + + K ++K+ + DITINIGK APIPE PIPGE WKE+RHDNTV
Sbjct: 436 FRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIRHDNTV 495
Query: 600 TWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NAYTKDFTSK 659
TWLAFWNDPIN KEFKYVFLAASSSLKG+ +EKYEK+R LKDYI+ IR AYTKDF SK
Sbjct: 496 TWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTKDFASK 555
Query: 660 DSTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDS 719
DS +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ PN+L+F+FLGKDS
Sbjct: 556 DSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDFLGKDS 615
Query: 720 IKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNA 779
I+Y+N VEVE VF AI QF+SGK G DDLFD+LDT+KLNAHLK+LMPGLTAKVFRTYNA
Sbjct: 616 IRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVFRTYNA 675
Query: 780 SITLDDM-----LNRGTKDGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEK 839
SITLD+M L+R TK GDVAEK+V YQHANKEVAIICNHQRTVSKSH AQM RLNEK
Sbjct: 676 SITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMVRLNEK 735
Query: 840 ISELRDILKELKIDLDRAKKGKPPLKDADGKRKRNLTPE--------------------- 899
I EL+ +LKEL+ DL R KGKPPLK++DGK KRNL +
Sbjct: 736 IEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKWSETRR 790
Query: 900 -ALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIF 939
++ KKI+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VPIEKIF
Sbjct: 796 LSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVPIEKIF 790
BLAST of Clc10G04860 vs. ExPASy Swiss-Prot
Match:
Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)
HSP 1 Score: 510.0 bits (1312), Expect = 5.7e-143
Identity = 357/951 (37.54%), Postives = 498/951 (52.37%), Query Frame = 0
Query: 54 RPGSDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDS 113
+P + + NG + K++TP PK +P+ + + K I P KP+ ++ SDS
Sbjct: 46 QPNKKLKAENG----SIKKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS---SDS 105
Query: 114 MGSRPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVK 173
S + + + D+SDS+ D S +S+ P K P+ P
Sbjct: 106 DSS-------------DSSSDSDSDSSDSDSDSSSSDSSSSDSDVPKKKPISKIP----N 165
Query: 174 KPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILS 233
KPSG S K + + K KP PKV S+S D S
Sbjct: 166 KPSGTVSKTSKNSPKTTTTTTTTTTTVK------SKP-APKVKPEQSSSDDS------SS 225
Query: 234 SKRPLPSADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEIDDDDDIPISQRMKKISA 293
S DS S S +KPK S P + K+EI + + + +
Sbjct: 226 DSSDDSSDDSSSDSSSEEEKPKKKPSKKPAA-------VKKEITVKKETTTTTKKTPVKK 285
Query: 294 PVNKSSSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQK--------- 353
V +K T +K V + +K K K + L +G++
Sbjct: 286 EVEVKKETKTTTTKKVKKEESDDDGEDEKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKW 345
Query: 354 -----------KWTTLVHNGVIFPPPYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDT 413
KWTTL+HNG +FPP Y HG+K +Y GK V LTP+QEEVAT +A +T
Sbjct: 346 WDKEDDEDDGTKWTTLIHNGPVFPPAYVPHGIKFMYDGKVVKLTPKQEEVATFYANYLET 405
Query: 414 DYMLKPKFKENFWNDWRKLL--GKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKA 473
D++ K FK+NF+ +++ LL + +++ +D CDF+ I+ +T+KE++K + EEK A
Sbjct: 406 DHVKKQAFKDNFFKEFKGLLTSEQKEIVKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDA 465
Query: 474 LKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLH 533
EK K +EK+ +A +DG KEK
Sbjct: 466 ETAEKKKIQEKHGFATIDGFKEK------------------------------------- 525
Query: 534 FMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECP 593
VGN+ +EPPGLF GRG+HPK G LK+RI PSD+T+NIG+G IP P
Sbjct: 526 -------------VGNYMIEPPGLFLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSP 585
Query: 594 IPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYI 653
IPGE WKE+ H+NTVTWLAFW + +N FKYV+LA SS +KGQ+D++K+E AR LK+ I
Sbjct: 586 IPGEKWKEIVHNNTVTWLAFWRESVN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCI 645
Query: 654 KNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAV 713
+IR Y K F + + +RQ+AVA YLID LALR GNEK +D+ADTVGCC+L+VE++K
Sbjct: 646 GDIRKGYQKSFNDESTEKRQLAVALYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLE 705
Query: 714 PPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDL 773
N + +FLGKDS++Y NTV++ +K + F GK TD LFD L +LN HLK
Sbjct: 706 SNNTITLDFLGKDSMRYLNTVQIREDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQ 765
Query: 774 MPGLTAKVFRTYNASITLDDMLNR-GTKD-GDVAEKIVTYQHANKEVAIICNHQRTVSKS 833
M GL+AKVFRTYNASITL L++ TK+ + EKI+ Y N+EVAI+CNHQ+ K+
Sbjct: 766 MTGLSAKVFRTYNASITLQKELDKMDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPKN 825
Query: 834 HGAQMTRLNEKISELRDILKELKIDLDRAKKGKPP------------------------- 893
M++++EKI ELRD+ DL R K GK P
Sbjct: 826 LAETMSKIDEKIQELRDLQ-----DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAITN 885
Query: 894 LKDADGKR--------------KRNLTPEA---LEKKISQTNAKIEKIERDMKTKEDLKT 939
KD D ++ K P+A +++KI++ +I+K E K++LKT
Sbjct: 886 KKDKDKEKTDKEISFDVKAAFEKSRTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKT 893
BLAST of Clc10G04860 vs. ExPASy Swiss-Prot
Match:
P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)
HSP 1 Score: 496.1 bits (1276), Expect = 8.6e-139
Identity = 335/884 (37.90%), Postives = 470/884 (53.17%), Query Frame = 0
Query: 139 NSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDSDDEIPLSSKFQMKSASGAL 198
+S D LSIR + + + K DS + PLS KS S +
Sbjct: 2 SSSDSDSVSLSIRRRQRRGSSKRISM------KESDEESDSSENHPLSESLNKKSKSES- 61
Query: 199 GGKQHGFDEKKPLVPKVHQNGSTSRDK---LQKPAILSSKRPLPSADSHSSSQISMKKPK 258
+ K+ K + + S+S+ + + + + K+ + ++I+ PK
Sbjct: 62 -DEDDIPIRKRRASSKKNMSNSSSKKRAKVMGNGGLKNGKKTAVVKEEEDFNEIAKPSPK 121
Query: 259 LSDSSTPLSSKPVSLKA--KQEIDDDDDIPISQRMKKISAPVNKSSSSKQTVSKVVSSSI 318
S SK + A K+E D DD +P+ + + +S KS + S++
Sbjct: 122 HKRVSKANGSKNGAKSAVKKEESDTDDSVPL-RAVSTVSLTPYKSE-----LPSGASTTQ 181
Query: 319 KKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQ--THGVKILYKG 378
++ + + K+ S + D +KWTTL HNGVIF PPY+ VK++Y G
Sbjct: 182 NRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLEHNGVIFAPPYEPLPKNVKLIYDG 241
Query: 379 KPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGK---NHVIQNLDDCDFT 438
PV+L PE EEVA +A M +TD+ P F++NF+ D+ K+ + NH I+ CDFT
Sbjct: 242 NPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNFNHNIKEFSKCDFT 301
Query: 439 PIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWKN 498
++ ++ ++E+KK M E+KKA+K++K ++EEKY W ILDG KEK
Sbjct: 302 QMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRKEK-------------- 361
Query: 499 LVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGKL 558
VGNFR+EPPGLFRGRG HPK G L
Sbjct: 362 ------------------------------------VGNFRIEPPGLFRGRGSHPKTGSL 421
Query: 559 KRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAA 618
KRR+ P ITINIG+G P+PE P+PG W EV+HDNTVTWLA W++ IN KYVFLAA
Sbjct: 422 KRRVYPEQITINIGEGVPVPE-PLPGHQWAEVKHDNTVTWLATWHENIN-NNVKYVFLAA 481
Query: 619 SSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRAGN 678
SSLKGQSD +KYEK+R LKDYI +IR Y KD S+ + RQ A YLID ALRAGN
Sbjct: 482 GSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSELTVERQRGTAMYLIDVFALRAGN 541
Query: 679 EKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQF-QS 738
EK +DEADTVGCC+L+ E+V PP + F+FLGKDSI+Y N VEV+ VFK + F +
Sbjct: 542 EKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRP 601
Query: 739 GKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 798
K D +FD+L TN LN +L LM GL+AKVFRTYNAS T+ + L + K+ +A+KI+
Sbjct: 602 PKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTYNASYTMAEELKKMPKNLTLADKIL 661
Query: 799 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISEL---RDILKELKIDLD-RAKKGKP 858
Y AN+ VAI+CNHQR+V+K+H QM R E+I L R L+++ ++L+ + K KP
Sbjct: 662 FYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKALQYQRMRLRKMMLNLEPKLAKSKP 721
Query: 859 PL---------------------------------------------------------- 918
L
Sbjct: 722 ELLAKEEGITDSWIVKHHETLYELEKEKIKKKFDRENEKLAAEDPKSVLPESELEVRLKA 781
Query: 919 -------KDADGKRKR----NLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 939
DA+ K K+ + E LEK++++ N +I + M K++ KT ALGTSK
Sbjct: 782 ADELKKALDAELKSKKVDPGRSSMEQLEKRLNKLNERINVMRTQMIDKDENKTTALGTSK 814
BLAST of Clc10G04860 vs. ExPASy TrEMBL
Match:
A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)
HSP 1 Score: 1585.1 bits (4103), Expect = 0.0e+00
Identity = 831/940 (88.40%), Postives = 854/940 (90.85%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR GSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 HLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDS 180
+LVNAAVKEEN +INP+ +NSDSEDDKPLSIRLKGNSNQPNKG V T PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLP 240
DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPA+LSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 SADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEI-DDDDDIPISQRMKKISAPVNKS 300
+ DSHS SQ S KKPKLSDSS+PLSSK VSLKAKQE+ DDDDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480
Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRG 540
VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD TRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889
Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Clc10G04860 vs. ExPASy TrEMBL
Match:
A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)
HSP 1 Score: 1573.9 bits (4074), Expect = 0.0e+00
Identity = 831/958 (86.74%), Postives = 854/958 (89.14%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GR GSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
S+NGQSSNNQKRMT LPKTSPMRSEIGS KA KSS IQEPL K LG +LK SDSM SRPK
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 HLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDS 180
+LVNAAVKEEN +INP+ +NSDSEDDKPLSIRLKGNSNQPNKG V T PKNKVKK SGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLP 240
DDE+PLSSKFQMK ASG +G KQ GFDEKKPLVPKVHQNGSTSRDKLQKPA+LSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 SADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEI-DDDDDIPISQRMKKISAPVNKS 300
+ DSHS SQ S KKPKLSDSS+PLSSK VSLKAKQE+ DDDDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK---- 480
Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRG 540
VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD TRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
DG+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 DGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
Query: 901 DPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMDVDPDFRF 939
DPRITVAWCKRHEVPIEK IFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMDVDPDFRF 907
BLAST of Clc10G04860 vs. ExPASy TrEMBL
Match:
A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)
HSP 1 Score: 1569.7 bits (4063), Expect = 0.0e+00
Identity = 821/939 (87.43%), Postives = 851/939 (90.63%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRPGSD C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
S+NGQSSN QKRMT LPKTSPMR EIGS KA+KSSIQEPLVK LGTTLKVSDSM SRPK
Sbjct: 61 SANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDSD 180
L NAAVKEEN +I+PT +NSDSEDDKPLSIRLKGNSN P+KGP+PT KNK+KK S DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLPS 240
DE+PLSSKFQMKSASG LGGKQHGF EKKPL KVHQNGSTSRDKLQKPA++SSKRPLP+
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQE-IDDDDDIPISQRMKKISAPVNKSS 300
ADSHSSSQIS KKPKLSDSSTPLS+ VSLKAKQE +DDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLT EQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480
Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGR 540
VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540
Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600
Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660
EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660
Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887
Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
BLAST of Clc10G04860 vs. ExPASy TrEMBL
Match:
A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)
HSP 1 Score: 1569.3 bits (4062), Expect = 0.0e+00
Identity = 821/940 (87.34%), Postives = 849/940 (90.32%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GR GSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSS-IQEPLVKPLGTTLKVSDSMGSRPK 120
S+NGQSSNNQKRMT LPKTSPMRSEIGS KAS+SS I EPL K LGT+LK S SM SRPK
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120
Query: 121 HLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDS 180
LVNA VKEEN +INP+ +NSDSEDDKPLSIRLKGN NQPNKG V T PKNKVKK DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180
Query: 181 DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLP 240
DDE+PLSSKFQMK ASG LG KQHGFDEKKPLVPKVHQNGSTSRDKLQKPA+LSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 SADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEI-DDDDDIPISQRMKKISAPVNKS 300
+ DSHS SQ KKPKL+DSS+PL+SK VSLKAKQE+ DDDDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILI 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEK---- 480
Query: 481 YHFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRG 540
VGNFRVEPPGLFRG
Sbjct: 481 ----------------------------------------------VGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGE WKEVRHDNTVTWLAFWNDPINQ
Sbjct: 541 RGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD TRRQIAVATYL
Sbjct: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YENTVEVELPV
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
FKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK
Sbjct: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
Query: 781 DGDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
+G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA
Sbjct: 781 NGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 900
KKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 889
Query: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 DPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Clc10G04860 vs. ExPASy TrEMBL
Match:
A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)
HSP 1 Score: 1568.9 bits (4061), Expect = 0.0e+00
Identity = 821/939 (87.43%), Postives = 851/939 (90.63%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRPGSDVC 60
MAVETFGKA MF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGRPGSD C
Sbjct: 1 MAVETFGKATMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 SSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGSRPKH 120
S+NGQSSN QKRMT LPKTSPMR EIGS KASKSSIQEPLVK LGTTLKVSDSM SRPK
Sbjct: 61 STNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNKGPVPTAPKNKVKKPSGDSD 180
LVNAAVKEEN +I+P +NSDSEDDKPLSIRLKGNSN P+KGP+PT KNK+KK S DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAILSSKRPLPS 240
DE+PLSSKF+MKSASG LGGKQHGFDEKKPL KVHQNGSTS DKLQKPA++SSKRPLP+
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQE-IDDDDDIPISQRMKKISAPVNKSS 300
ADSHSSSQIS KKPKLSDSSTPLS+ VSLKAKQE +DDDD IPISQRM K+SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIY 480
QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEK----- 480
Query: 481 HFLLAFWKNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGR 540
VGNFRVEPPGLFRGR
Sbjct: 481 ---------------------------------------------VGNFRVEPPGLFRGR 540
Query: 541 GEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQK 600
GEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+ WKEVRHDNTVTWLAFWNDPIN K
Sbjct: 541 GEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPK 600
Query: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLI 660
EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TRRQIAVATYLI
Sbjct: 601 EFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLI 660
Query: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF
Sbjct: 661 DKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVF 720
Query: 721 KAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
KAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD
Sbjct: 721 KAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKD 780
Query: 781 GDVAEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
G+V+EKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK
Sbjct: 781 GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAK 840
Query: 841 KGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 900
KGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD
Sbjct: 841 KGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLD 887
Query: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 901 PRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
BLAST of Clc10G04860 vs. TAIR 10
Match:
AT5G55300.1 (DNA topoisomerase I alpha )
HSP 1 Score: 1003.0 bits (2592), Expect = 1.5e-292
Identity = 577/992 (58.17%), Postives = 683/992 (68.85%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
S V S NG TP KTS ++S + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGN----------SNQP---NKGP 180
K ++ E DSEDD+PLS L GN S QP N G
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPTAPKNKVKKPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSR 240
P +++ K +SDDE P+SS F+ K SG GG Q DEKKPLV K+HQNGST +
Sbjct: 181 RPLDRASRIIK--DESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPAILSSKRPLPSADSHSSSQISMKKPKLSDSSTPLSSKPVS-------------- 300
+++ +L KRPL + S+ Q S+KK K+S S T + K S
Sbjct: 241 NEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300
Query: 301 --LKAKQ--------EIDDDDDIPISQRMKKISAPVNKSSSSKQTVS------------- 360
+KAKQ + DDDDD+PIS+R K S+ N SS+ + V
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360
Query: 361 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 420
VVS + K +KK K SKY S+K PS+GDGQKKWTTLVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420
Query: 421 ILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
ILYKGKPVDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480
Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFW 540
FTPIY+W+ EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEK------------ 540
Query: 541 KNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMG 600
+GNFRVEPPGLFRGRGEHPKMG
Sbjct: 541 --------------------------------------IGNFRVEPPGLFRGRGEHPKMG 600
Query: 601 KLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFL 660
KLK+RI P +IT+NIGKGAPIPECPI GE WKEV+HDNTVTWLAFW DPIN KEFKYVFL
Sbjct: 601 KLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFL 660
Query: 661 AASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRA 720
A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVATYLIDKLALRA
Sbjct: 661 GAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRA 720
Query: 721 GNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQ 780
GNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ
Sbjct: 721 GNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQ 780
Query: 781 SGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKI 840
+GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KI
Sbjct: 781 AGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKI 840
Query: 841 VTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLK 900
V YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 841 VVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLE 900
Query: 901 DADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAW 939
+DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAW
Sbjct: 901 GSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAW 913
BLAST of Clc10G04860 vs. TAIR 10
Match:
AT5G55300.2 (DNA topoisomerase I alpha )
HSP 1 Score: 998.0 bits (2579), Expect = 4.9e-291
Identity = 575/989 (58.14%), Postives = 680/989 (68.76%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
S V S NG TP KTS ++S + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGN----------SNQP---NKGP 180
K ++ E DSEDD+PLS L GN S QP N G
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPTAPKNKVKKPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSR 240
P +++ K +SDDE P+SS F+ K SG GG Q DEKKPLV K+HQNGST +
Sbjct: 181 RPLDRASRIIK--DESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPAILSSKRPLPSADSHSSSQISMKKPKLSDSSTPLSSKPVS-------------- 300
+++ +L KRPL + S+ Q S+KK K+S S T + K S
Sbjct: 241 NEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVS 300
Query: 301 --LKAKQ--------EIDDDDDIPISQRMKKISAPVNKSSSSKQTVS------------- 360
+KAKQ + DDDDD+PIS+R K S+ N SS+ + V
Sbjct: 301 PKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKAR 360
Query: 361 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 420
VVS + K +KK K SKY S+K PS+GDGQKKWTTLVHNGVIFPPPY+ HG+K
Sbjct: 361 NVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIK 420
Query: 421 ILYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
ILYKGKPVDLT EQEEVATMFAVM++TDY KP+F+ENFWNDWR+LLGK HVIQ LDDCD
Sbjct: 421 ILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCD 480
Query: 481 FTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFW 540
FTPIY+W+ EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK
Sbjct: 481 FTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEK------------ 540
Query: 541 KNLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMG 600
+GNFRVEPPGLFRGRGEHPKMG
Sbjct: 541 --------------------------------------IGNFRVEPPGLFRGRGEHPKMG 600
Query: 601 KLKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFL 660
KLK+RI P +IT+NIGKGAPIPECPI GE WKEV+HDNTVTWLAFW DPIN KEFKYVFL
Sbjct: 601 KLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFL 660
Query: 661 AASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRA 720
A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVATYLIDKLALRA
Sbjct: 661 GAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRA 720
Query: 721 GNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQ 780
GNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ
Sbjct: 721 GNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQ 780
Query: 781 SGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKI 840
+GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KI
Sbjct: 781 AGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKI 840
Query: 841 VTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLK 900
V YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 841 VVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLE 900
Query: 901 DADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAW 936
+DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAW
Sbjct: 901 GSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAW 910
BLAST of Clc10G04860 vs. TAIR 10
Match:
AT5G55310.1 (DNA topoisomerase 1 beta )
HSP 1 Score: 994.2 bits (2569), Expect = 7.1e-290
Identity = 570/991 (57.52%), Postives = 687/991 (69.32%), Query Frame = 0
Query: 1 MAVETFGKAPMFD-----DDDDEDNMPIVFKRSS-----ASRQNQLDSEIKHSQSQRLNK 60
MA E F K + + +D+DED++P+VFKR+S +R + +++ +++S +
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIG--ST 60
Query: 61 LSGRPGSDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKV 120
S P +S +S+++ R + + P S + +S+++ PL K
Sbjct: 61 KSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSV-----QRSTLKSPLRDDRSVVAKE 120
Query: 121 SDSMGSRPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGNSNQPNK-------GP 180
+ G P +++ +DD DSEDDKPLS RLK +S + K G
Sbjct: 121 RNGFGKAP-------------SVSKSDD-EDSEDDKPLSARLKLDSKEVTKQPSSSGRGS 180
Query: 181 VPTAPKNKVKKPSGDS--------DDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKV 240
A + +P G S D+ P+SS Q K++ G K ++K+PLV +
Sbjct: 181 TQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNI 240
Query: 241 HQNG---STSRDKLQKPAILSSKRPLPSADSHSSSQISMKKPKLS--------------- 300
+NG T Q PA KRPL SS+Q S+K+PKLS
Sbjct: 241 DRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHIS 300
Query: 301 ----------DSSTPLSSKPVSLKAKQEIDDDDDIPISQRMKKISAPVNKSSSSKQTVSK 360
D+S PL + ++K + D DD +PI+ RMK S+ NKSSS+K + SK
Sbjct: 301 ATQDAKGKNLDASKPLRANQATVK-EDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSSK 360
Query: 361 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 420
+++SS + +K K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVKI
Sbjct: 361 MIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVKI 420
Query: 421 LYKGKPVDLTPEQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDF 480
L++GKPVDLTPEQEEVATMFAVM++T+Y KPKF+ENFWNDWRKLLGKNH+I++LDDCDF
Sbjct: 421 LFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDF 480
Query: 481 TPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVILIYHFLLAFWK 540
TPIY+WY EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E+
Sbjct: 481 TPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRER------------- 540
Query: 541 NLVLRIFFVYFDNLPSDNVIDLLHFMVITNQQLATTEVGNFRVEPPGLFRGRGEHPKMGK 600
+GNFRVEPPGLFRGRGEHPKMGK
Sbjct: 541 -------------------------------------IGNFRVEPPGLFRGRGEHPKMGK 600
Query: 601 LKRRIRPSDITINIGKGAPIPECPIPGESWKEVRHDNTVTWLAFWNDPINQKEFKYVFLA 660
LK+RIRP DITINIGK APIPECPIPGE WKEV+HDNTVTWLAFW+DPIN KEFKYVFLA
Sbjct: 601 LKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLA 660
Query: 661 ASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRAG 720
ASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATYLIDKLALRAG
Sbjct: 661 ASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAG 720
Query: 721 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 780
NEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE V+KAI QFQ+
Sbjct: 721 NEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 780
Query: 781 GKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 840
GKS TDDLFD+LDT+KLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV EK+V
Sbjct: 781 GKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPEKVV 840
Query: 841 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKD 900
YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL IDLDRAKKG+ PL
Sbjct: 841 VYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTPLMG 900
Query: 901 ADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 939
+DGKRKRNLTPEALEKKI QT KIEK+ERDM+TKED+KTVALGTSKINY+DPRITVAWC
Sbjct: 901 SDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVAWC 911
BLAST of Clc10G04860 vs. TAIR 10
Match:
AT5G55300.3 (DNA topoisomerase I alpha )
HSP 1 Score: 425.2 bits (1092), Expect = 1.3e-118
Identity = 208/271 (76.75%), Postives = 244/271 (90.04%), Query Frame = 0
Query: 668 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 727
++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350
Query: 728 GKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 787
GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410
Query: 788 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKD 847
YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL+++LKELK +LDRAKKGKPPL+
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470
Query: 848 ADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 907
+DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530
Query: 908 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 939
KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561
HSP 2 Score: 102.1 bits (253), Expect = 2.6e-21
Identity = 109/306 (35.62%), Postives = 154/306 (50.33%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRPG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60
Query: 61 SDVCSSNGQSSNNQKRMTPLPKTSPMRSEIGSPKASKSSIQEPLVKPLGTTLKVSDSMGS 120
S V S NG TP KTS ++S + S + S + PL + +T+K +
Sbjct: 61 SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120
Query: 121 RPKHLVNAAVKEENFTINPTDDNSDSEDDKPLSIRLKGN----------SNQP---NKGP 180
K ++ E DSEDD+PLS L GN S QP N G
Sbjct: 121 -QKDQSECKIEHE-----------DSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPTAPKNKVKKPSGDSDDEIPLSSKFQMKSASGALGGKQHGFDEKKPLVPKVHQNGSTSR 240
P +++ K +SDDE P+SS F+ K SG GG Q DEKKPLV K+HQNGST +
Sbjct: 181 RPLDRASRIIK--DESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVK 240
Query: 241 DKLQKPAILSSKRPLPSADSHSSSQISMKKPKLSDSSTPLSSKPVSLKAKQEIDDDDDIP 290
+++ +L KRPL + S+ Q S+KK K+S S T + K S+ K+EIDD +
Sbjct: 241 NEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSV--KKEIDDKGRVL 275
BLAST of Clc10G04860 vs. TAIR 10
Match:
AT4G26701.1 (DNA binding;DNA topoisomerase type Is )
HSP 1 Score: 129.0 bits (323), Expect = 2.0e-29
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0
Query: 871 KIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 930
KIEK+ERDM+TKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2 KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61
Query: 931 DVDPDFRF 939
DV+P F F
Sbjct: 62 DVEPHFTF 69
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038904656.1 | 0.0e+00 | 89.78 | DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DN... | [more] |
| XP_008449103.1 | 0.0e+00 | 88.40 | PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DN... | [more] |
| XP_023553297.1 | 0.0e+00 | 87.65 | DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo] | [more] |
| KAG7015549.1 | 0.0e+00 | 87.43 | DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| KAA0065816.1 | 0.0e+00 | 86.74 | DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomeras... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| P30181 | 2.2e-291 | 58.17 | DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1 | [more] |
| Q9FJ79 | 1.0e-288 | 57.52 | DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1 | [more] |
| P93119 | 5.4e-234 | 56.16 | DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1 | [more] |
| Q54RC3 | 5.7e-143 | 37.54 | DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1 | [more] |
| P07799 | 8.6e-139 | 37.90 | DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3BM72 | 0.0e+00 | 88.40 | DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1 | [more] |
| A0A5A7VHI9 | 0.0e+00 | 86.74 | DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G... | [more] |
| A0A6J1EWI0 | 0.0e+00 | 87.43 | DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1 | [more] |
| A0A0A0L2W2 | 0.0e+00 | 87.34 | DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1 | [more] |
| A0A6J1KZE0 | 0.0e+00 | 87.43 | DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1 | [more] |
