Clc08G17320 (gene) Watermelon (cordophanus) v2

Overview
NameClc08G17320
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionSqualene monooxygenase
LocationClcChr08: 27648804 .. 27652495 (+)
RNA-Seq ExpressionClc08G17320
SyntenyClc08G17320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGTGGCTGTGGCTGCCTCTCCGCCTCACAAATTCTATAAAACCGCCTTCTCTTTCTCAATTTTCACTCCCCGTAGCGATTTTTCTTTCGTCGTCGCCGCCGCAGGGTTCTCCCCTGAGGTTGCTGTGATTTGCCATTCTCTTGAACCAGGAAACAATGGTGGATCACTGCGCCTTCGGCTGGATCTTGTCCGCCTTCCTCGCCTTCGCCATTGCCTTCTCTTTCTTCCTCGCTTCCAGGAGGAACCGCCGAGTAGGAGGTGCCGATTCCACGCCGAGGACCGACTGTGTCAGAAGCTCTGCATCCACCAATGGAGAATGCAGATCCGTCGACGGCGACGCCGACGTCATCATCGTCGGAGCCGGTGTTGCTGGTTCCGCTCTTGCTCACACTCTCGGCAAGGTACAATTGGTTTTTATCGCTTTATTTTGAATTTTTAATCTCTTCTTTCTCAGCGCCATTTGATTATTAGTATAATTGATATTTGATTCATAGGAACCCTTAGAATGAAAGAATCCATCGTAGAGTTATGAGAGGAAAAACAATGGAAAGAAGAAAGAAATTAAAAACTTCGGGTAGTAAATTGCTCTTCCTTCCATGTAACCAATGAAATGACTGGATGATTGTTTTTTCTATCTTTATTTCTTATTGGTGCAAATATATAGAAATAAAAATTTTACGCAGCCAACAGTCGATTTTTATAATGGATAAAATCGCTGCTACGTATTTTCATGTAAAATGGTTTTAATTATTGTTGACCTAAGGACAAGGTACGAATTTATATTTTAAATCTTAACCCAAAACCCTATTTATTTATTTATTTATTTTATGTAATTGTTGCTCATCATAGACAACAATTAAATTATCATTTCAAAAATTTGATTTTTAAATTTGAAAGAGAGAAAAAAAAAAGGTTTATATATATATATATTGTGTTCTTTTTGTTATTTTTACAATGGAAGCAAAGCAAGCTACTGAGATCAAAATTGTTAGGATGGTCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCGGATAGAATCGTCGGTGAATTACTGCAGCCTGGGGGCTACCTCAAATTGATTGAGCTAGGATTGCAAGGTTTGTAATTACCCATAGCAAATTTCTTTTGCAACTCTAATTATTTTCATTCTTTGAAGTACAAAATATTCATCAATTAATTTAGGAAAACACATGTTTGTAATACAGATTGCGTTGAGGAAATTGATGCACAGAAGGTTTATGGCTATGCCCTTTTCAAGGATGGCAAGAATACTCGACTTTCTTACCCATTGGAAAATTTTCACTCTGATGTATCTGGAAGAAGCTTTCACAATGGACGCTTCATACAGAGAATGAGGAAGAAAGCTGCTTCACTTCCCAAGTATGTTCATTTCGTATTTGTTTTCTGCTCCTATAATTCATAAGAGTATATGGGTTTTAGTTAAACTATTCTATACAAGTAAATTATCCATAACCCATCAACTTAAGCTTTTAGATTGGCCATTATAGTGTTCACTGTTGAAGATCTTAAGAATTTCCCTTGATCAGATGTTAAATGTTAAATTTGGTCTCTTGGAGTAATTATTCTATGAATGGAGTATACAATTTTGCATTCCGAAGATTCATGTCCTGAGTTGTTCTTTGATATGAAACTTATGCTCTTGGAACGTTAGGGATTTGATCGTATTGTTGAGCAGACTCGGATATTATGAGGTTGAAATTAATGTTACTTCTGCATATGTAAATGGATTTAAGGCAAATTTCTTATGTTTGGTTCAGAGCTGCCATATTGAATCTGATTAAAGTCATAGAGACAAACATTCACCATTGATACTTGTGTATTTCTTGTGATTGGATTAACGATTAACTACTCGGGTTTCACTCATCAATGAAACTTCTTACATCCTCTGTTTCTAGTGTGAGATTGGAGCAAGGGACAGTGACTTCGTTACTGGAAAAAAAGGGAACAATCAAAGGCGTGCACTATAAGTCCAAAAATGGTGAAGAAAAAACAGCATATGCCCCTCTGACCATTGTTTGTGATGGTTGCTTCTCAAATTTGCGTCGCTCTCTTTGCAACCCTATGGTAAGGGGATTCCATTATATGTCAGTGGTACCCCAAAATAGACAATGATTTTCTCTTGGGATTTGATTGGCTGCAGAATGATTCTAATTACATTTTGCTAACTGCATGAAAACAGTGAATAAGTTTTTCAAATTCCGAGCCCTTTATCTGCTCTATCTTTTGCAGGTTGATGTTCCATCGTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGGCATGTTGTTCTTGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGCAGTACCGAGATCCGTTGTCTAGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATCTGAAGACCGTAGTAGCTCCTCAGGTACAATTTTTCAACAATCTCATTCATGCCGAAGTATGTGTTCTAGTTCTCTGAACTGATGAATTTACCATTACTCGTGCAGGTTCCTCCCGAAATCCATGATGCATTTATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCCGCTCCCCAACCGACACCTGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCTCTCACCGGTGGAGGAATGACAGTAGCTTTGTCTGATATTGTTGTGCTCAGGAACCTCATCAAGCCATTGAAAGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGAGAATGGTATAACTTTAGTTTATGTTGTTGTCGGCAATCCGAAAAGTCATTTTACCAATAGGCCGTAAGTAGAAGCTCTAATATACTCGTCTCAAAAACTGCAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTGTTTTGTGCATCGCCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTCTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTCTCCATTTCTTTGCGGTCGCAATATATGGAGTCGGTCGCTTACTGCTTCCATTTCCTTCTCTGAAAGGCATCTGGATTGGAGCAAGATTGATCTATGTGAGTTTCGAACAAACAAAACATCATCATATCATGTTTTCCTCTTAGACAAGTAATGAGTAATGAGAAATGAATTGGTTTGTTTATTGCAGAGCGCATCAGGAATCATATTTCCAATAATAAAGGCTGAAGGAGTTAGGCAGATGTTCTTCCCAGCAACGGTTCCTGCTTATTACAGAACCCCACCAGTTTTTAAACCCATAGCTTGAAAGTAATGATGCAGAGAGAAAGGATGAAGTTGTTGGGGATTGTTTGTATCATATCTGAGGAGGAAGAAGAGGCATTACAAGGAAGACAAGTCAAGTTTTTGTTGTTAGATTTTGGTTTTAATTGTGGATTAATAAAACTGTGTACTTGTGAACTGGAGCCAGGAGTGTAAATATTCTTAATTCATTTGAGGAGCACCCAATCATTCATGTATTGTCAAACCGCTTGGGAACGCTTTAATAGACTAGAGAGACTTCCTTTTGTTTAAT

mRNA sequence

ATGTATGTGGCTGTGGCTGCCTCTCCGCCTCACAAATTCTATAAAACCGCCTTCTCTTTCTCAATTTTCACTCCCCGTAGCGATTTTTCTTTCGTCGTCGCCGCCGCAGGGTTCTCCCCTGAGGAAACAATGGTGGATCACTGCGCCTTCGGCTGGATCTTGTCCGCCTTCCTCGCCTTCGCCATTGCCTTCTCTTTCTTCCTCGCTTCCAGGAGGAACCGCCGAGTAGGAGGTGCCGATTCCACGCCGAGGACCGACTGTGTCAGAAGCTCTGCATCCACCAATGGAGAATGCAGATCCGTCGACGGCGACGCCGACGTCATCATCGTCGGAGCCGGTGTTGCTGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCGGATAGAATCGTCGGTGAATTACTGCAGCCTGGGGGCTACCTCAAATTGATTGAGCTAGGATTGCAAGATTGCGTTGAGGAAATTGATGCACAGAAGGTTTATGGCTATGCCCTTTTCAAGGATGGCAAGAATACTCGACTTTCTTACCCATTGGAAAATTTTCACTCTGATGTATCTGGAAGAAGCTTTCACAATGGACGCTTCATACAGAGAATGAGGAAGAAAGCTGCTTCACTTCCCAATGTGAGATTGGAGCAAGGGACAGTGACTTCGTTACTGGAAAAAAAGGGAACAATCAAAGGCGTTGATGTTCCATCGTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGGCATGTTGTTCTTGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGCAGTACCGAGATCCGTTGTCTAGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATCTGAAGACCGTAGTAGCTCCTCAGGTTCCTCCCGAAATCCATGATGCATTTATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCCGCTCCCCAACCGACACCTGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCTCTCACCGGTGGAGGAATGACAGTAGCTTTGTCTGATATTGTTGTGCTCAGGAACCTCATCAAGCCATTGAAAGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGAGAATGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTGTTTTGTGCATCGCCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTCTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTCTCCATTTCTTTGCGGTCGCAATATATGGAGTCGGTCGCTTACTGCTTCCATTTCCTTCTCTGAAAGGCATCTGGATTGGAGCAAGATTGATCTATAGCGCATCAGGAATCATATTTCCAATAATAAAGGCTGAAGGAGTTAGGCAGATGTTCTTCCCAGCAACGGTTCCTGCTTATTACAGAACCCCACCAGTTTTTAAACCCATAGCTTGAAAGTAATGATGCAGAGAGAAAGGATGAAGTTGTTGGGGATTGTTTGTATCATATCTGAGGAGGAAGAAGAGGCATTACAAGGAAGACAAGTCAAGTTTTTGTTGTTAGATTTTGGTTTTAATTGTGGATTAATAAAACTGTGTACTTGTGAACTGGAGCCAGGAGTGTAAATATTCTTAATTCATTTGAGGAGCACCCAATCATTCATGTATTGTCAAACCGCTTGGGAACGCTTTAATAGACTAGAGAGACTTCCTTTTGTTTAAT

Coding sequence (CDS)

ATGTATGTGGCTGTGGCTGCCTCTCCGCCTCACAAATTCTATAAAACCGCCTTCTCTTTCTCAATTTTCACTCCCCGTAGCGATTTTTCTTTCGTCGTCGCCGCCGCAGGGTTCTCCCCTGAGGAAACAATGGTGGATCACTGCGCCTTCGGCTGGATCTTGTCCGCCTTCCTCGCCTTCGCCATTGCCTTCTCTTTCTTCCTCGCTTCCAGGAGGAACCGCCGAGTAGGAGGTGCCGATTCCACGCCGAGGACCGACTGTGTCAGAAGCTCTGCATCCACCAATGGAGAATGCAGATCCGTCGACGGCGACGCCGACGTCATCATCGTCGGAGCCGGTGTTGCTGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCGGATAGAATCGTCGGTGAATTACTGCAGCCTGGGGGCTACCTCAAATTGATTGAGCTAGGATTGCAAGATTGCGTTGAGGAAATTGATGCACAGAAGGTTTATGGCTATGCCCTTTTCAAGGATGGCAAGAATACTCGACTTTCTTACCCATTGGAAAATTTTCACTCTGATGTATCTGGAAGAAGCTTTCACAATGGACGCTTCATACAGAGAATGAGGAAGAAAGCTGCTTCACTTCCCAATGTGAGATTGGAGCAAGGGACAGTGACTTCGTTACTGGAAAAAAAGGGAACAATCAAAGGCGTTGATGTTCCATCGTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGGCATGTTGTTCTTGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGCAGTACCGAGATCCGTTGTCTAGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATCTGAAGACCGTAGTAGCTCCTCAGGTTCCTCCCGAAATCCATGATGCATTTATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCCGCTCCCCAACCGACACCTGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCTCTCACCGGTGGAGGAATGACAGTAGCTTTGTCTGATATTGTTGTGCTCAGGAACCTCATCAAGCCATTGAAAGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGAGAATGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTGTTTTGTGCATCGCCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTCTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTCTCCATTTCTTTGCGGTCGCAATATATGGAGTCGGTCGCTTACTGCTTCCATTTCCTTCTCTGAAAGGCATCTGGATTGGAGCAAGATTGATCTATAGCGCATCAGGAATCATATTTCCAATAATAAAGGCTGAAGGAGTTAGGCAGATGTTCTTCCCAGCAACGGTTCCTGCTTATTACAGAACCCCACCAGTTTTTAAACCCATAGCTTGA

Protein sequence

MYVAVAASPPHKFYKTAFSFSIFTPRSDFSFVVAAAGFSPEETMVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVRLEQGTVTSLLEKKGTIKGVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFKPIA
Homology
BLAST of Clc08G17320 vs. NCBI nr
Match: XP_038886247.1 (squalene monooxygenase SE1-like [Benincasa hispida])

HSP 1 Score: 935.3 bits (2416), Expect = 2.4e-268
Identity = 471/529 (89.04%), Postives = 482/529 (91.12%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVDHCAFGWILSA  AF IA SFFLA RRNRRV G  STPR+DC+RSS++TNGECRS+DG
Sbjct: 1   MVDHCAFGWILSALFAFVIALSFFLAPRRNRRVEGTHSTPRSDCLRSSSTTNGECRSIDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTIKG                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEVKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KTVVAPQVP EIHDAF+AAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPREIHDAFVAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFKPI 537
            KGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPVFKP+
Sbjct: 481 PKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPM 529

BLAST of Clc08G17320 vs. NCBI nr
Match: XP_004142907.1 (squalene monooxygenase SE1 [Cucumis sativus] >KGN62420.1 hypothetical protein Csa_018728 [Cucumis sativus])

HSP 1 Score: 913.3 bits (2359), Expect = 9.8e-262
Identity = 462/526 (87.83%), Postives = 474/526 (90.11%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVDHC FGWI SAFLAF IAFSFFL+ R+NRR  G +STPR DC+ SSA+TNGECRSVDG
Sbjct: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMR+KAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTI G                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF 534
            KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF 526

BLAST of Clc08G17320 vs. NCBI nr
Match: KAG6598316.1 (Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029287.1 hypothetical protein SDJN02_07625 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 913.3 bits (2359), Expect = 9.8e-262
Identity = 463/530 (87.36%), Postives = 480/530 (90.57%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVD+CAFGWIL+A L  AIA SFF++ RRNRR GGADSTPR++ VRSS++TNGECRSVDG
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRSSSTTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNT+LSYPLE F SDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTIKG                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFKPIA 538
            KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV K IA
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Clc08G17320 vs. NCBI nr
Match: XP_022996831.1 (squalene monooxygenase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 912.5 bits (2357), Expect = 1.7e-261
Identity = 462/530 (87.17%), Postives = 480/530 (90.57%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVD+CAFGWIL+A L  AIA SFF++ RRNRR GGADSTPR++ VRSS++TNGECRSVDG
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRSSSTTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNT+LSYPLE F SDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTIKG                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFKPIA 538
            KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV K IA
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA 529

BLAST of Clc08G17320 vs. NCBI nr
Match: XP_008444517.1 (PREDICTED: squalene monooxygenase-like [Cucumis melo])

HSP 1 Score: 911.8 bits (2355), Expect = 2.9e-261
Identity = 463/525 (88.19%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVDHCAFGWI SA LAF IA S FL+  RNRRV G DSTPR+  V SSA+TNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTI G                                    VDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSY 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 533
           LKGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of Clc08G17320 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 729.6 bits (1882), Expect = 2.6e-209
Identity = 372/528 (70.45%), Postives = 420/528 (79.55%), Query Frame = 0

Query: 53  ILSAFLAFAIAFSFFLASRRNRR---VGGADSTPRTD---CVRSSASTNGECRSVD---G 112
           + +A L F      F   RR RR    GGA S    +    + SS+  NG C   D    
Sbjct: 16  MFAATLLFGFVLYLFTLRRRRRRREKKGGAGSMEIINGAYKMTSSSEVNGHCTPEDIAGS 75

Query: 113 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 172
             DVIIVGAGVAGSALA+TL KDGRRVHVIERDLTE DRIVGELLQPGGYLKL+ELGL+D
Sbjct: 76  SDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLVELGLED 135

Query: 173 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 232
           CV EIDAQ+V+GYAL+ DGKNTRLSYPLE FH+DV+GRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 136 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAASLPNVR 195

Query: 233 LEQGTVTSLLEKKGTIKG-------------------------------------VDVPS 292
           +EQGTVTSL+E+KGT+KG                                     VDVPS
Sbjct: 196 MEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNPKVDVPS 255

Query: 293 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 352
           CFVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQKVPSI+NGE+  Y
Sbjct: 256 CFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIANGELAHY 315

Query: 353 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 412
           LKT VAPQ+PPE++ +FIAAI+KG I+TMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 316 LKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMRHPLTGG 375

Query: 413 GMTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCAS 472
           GMTVALSDIV++R+L++PL+DL+D+ TLCKYLESFYTLR PVASTINTLAGALYKVFCAS
Sbjct: 376 GMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 435

Query: 473 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 532
           PD+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL  HFFAVAIYGVGRLL+PFP
Sbjct: 436 PDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGRLLIPFP 495

Query: 533 SLKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFK 535
           S + +W+GARLI  ASGIIFPIIK+EGVRQMFFPATVPAYYR PP+ K
Sbjct: 496 SPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPITK 543

BLAST of Clc08G17320 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 1.3e-208
Identity = 375/552 (67.93%), Postives = 428/552 (77.54%), Query Frame = 0

Query: 19  SFSIFTPRSDFSFVVAAAGFSPEETMVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGG 78
           S S  T  +  SF+ A+A       ++D    GWI     AF   F   L  +R R    
Sbjct: 4   SSSTTTTDTLHSFMEASA------LLIDQYFLGWI----FAFLFGFLLLLNFKRKREKNN 63

Query: 79  ADSTPRTDCVRSSASTNG--ECRSVDGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERD 138
           +      D       +NG     ++ G  DVIIVGAGVAGSALA+TL  DGRRVHVIERD
Sbjct: 64  STEFGTDD-------SNGYYTPENIAGSTDVIIVGAGVAGSALAYTLANDGRRVHVIERD 123

Query: 139 LTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHS 198
           LTE DRIVGELLQPGGYLKLIELGL+DCV EIDAQ+V+GYAL+ DGKNTRLSYPLE FHS
Sbjct: 124 LTEQDRIVGELLQPGGYLKLIELGLEDCVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHS 183

Query: 199 DVSGRSFHNGRFIQRMRKKAASLPNVRLEQGTVTSLLEKKGTIKG--------------- 258
           DV+GRSFHNGRF+QRMR+KAASLPNVR+EQGTVTSL+EKKG++KG               
Sbjct: 184 DVAGRSFHNGRFVQRMREKAASLPNVRMEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFA 243

Query: 259 ---------------------VDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPIS 318
                                V+VPSCFVGL+LEN  LP  NHGHV+L DPSPILFY IS
Sbjct: 244 PLTIVCDGCFSNLRRSLCNPKVEVPSCFVGLILENIDLPHINHGHVILADPSPILFYKIS 303

Query: 319 STEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSM 378
           STEIRCLVDVPGQKVP ISNGE+  YLKTVVAPQVP +++++FIAA++KGNIRTMPNRSM
Sbjct: 304 STEIRCLVDVPGQKVPCISNGELANYLKTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSM 363

Query: 379 PAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLIKPLKDLNDAPTLCKYLES 438
           PA P PTPGALL+GDAFNMRHPLTGGGMTVALSDIV++R+L++PL+DL+D+ TLCKYLES
Sbjct: 364 PADPHPTPGALLLGDAFNMRHPLTGGGMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLES 423

Query: 439 FYTLRMPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLN 498
           FYTLR PVASTINTLAGALYKVFCASPD+AR+EMR ACFDYLSLGGI S GP++LLSGLN
Sbjct: 424 FYTLRKPVASTINTLAGALYKVFCASPDKARQEMRNACFDYLSLGGICSQGPIALLSGLN 483

Query: 499 PRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLIYSASGIIFPIIKAEGVRQMFFP 533
           PRP+SL LHFFAVAIYGVGRLL+PFPS K +W+GARLI  ASGIIFPIIK+EG+RQMFFP
Sbjct: 484 PRPISLFLHFFAVAIYGVGRLLIPFPSPKRMWLGARLILGASGIIFPIIKSEGLRQMFFP 538

BLAST of Clc08G17320 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 3.0e-205
Identity = 367/525 (69.90%), Postives = 421/525 (80.19%), Query Frame = 0

Query: 50  FGWIL-----SAFLAFAIAFSFFLASRRN--RRVGGADSTPRTDCVRSSASTNGECRSVD 109
           + WIL     S  ++F   + FF+  +RN  R      ST  +D    S +  G     D
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDV--GSVNITG-----D 65

Query: 110 GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 169
             ADVI+VGAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGELLQPGGYLKL+ELG++
Sbjct: 66  TVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIE 125

Query: 170 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNV 229
           DCVEEIDAQ+VYGYALFK+GK  RL+YPLE FH DVSGRSFHNGRFIQRMR+KAASLPNV
Sbjct: 126 DCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNV 185

Query: 230 RLEQGTVTSLLEKKGTIKG------------------------------------VDVPS 289
           +LEQGTV SLLE+ GTIKG                                    V+VPS
Sbjct: 186 QLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPS 245

Query: 290 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 349
           CFVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ Y
Sbjct: 246 CFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNY 305

Query: 350 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 409
           LKTVVAPQ+P E++D+FIAA++KGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGG
Sbjct: 306 LKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGG 365

Query: 410 GMTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCAS 469
           GMTVAL+DIVVLRNL++PL+DL+D  +LCKYLESFYTLR PVA+TINTLA ALY+VFC+S
Sbjct: 366 GMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSS 425

Query: 470 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 529
            ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFP
Sbjct: 426 ENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFP 485

Query: 530 SLKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 532
           S K IW+GA+LI  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 486 SPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of Clc08G17320 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 1.4e-202
Identity = 359/523 (68.64%), Postives = 413/523 (78.97%), Query Frame = 0

Query: 45  VDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDGD 104
           VDHC    + + F+A   AF      RR  +        R   +   + T         D
Sbjct: 7   VDHCI---LTTTFVASLFAFLLLYVLRRRSKTIHGSVNVRNGTLTVKSGT---------D 66

Query: 105 ADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 164
            D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL+DC
Sbjct: 67  VDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDC 126

Query: 165 VEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVRL 224
           V++IDAQ+V GYALFKDGK+T+LSYPL+ F SDV+GRSFHNGRF+QRMR+KA+ LPNVR+
Sbjct: 127 VKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVRM 186

Query: 225 EQGTVTSLLEKKGTIKG------------------------------------VDVPSCF 284
           EQGTVTSL+E+ G IKG                                    V+VPS F
Sbjct: 187 EQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSNF 246

Query: 285 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 344
           VGLVLENC+LPF NHGHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+++GEM  +LK
Sbjct: 247 VGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHLK 306

Query: 345 TVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 404
           T+VAPQVPP+I DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGGGM
Sbjct: 307 TMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGGM 366

Query: 405 TVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASPD 464
           TVALSDIV+LR+L+ PL DL +  +L KY+ESFYTLR PVASTINTLAGALYKVF ASPD
Sbjct: 367 TVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASPD 426

Query: 465 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSL 524
            AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLVLHFFAVAI+GVGRLL+P PS+
Sbjct: 427 DARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPSV 486

Query: 525 KGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 532
           K +W+GARLI SASGIIFPIIKAEGVRQMFFP T+PA YR PP
Sbjct: 487 KRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of Clc08G17320 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 3.8e-200
Identity = 356/519 (68.59%), Postives = 410/519 (79.00%), Query Frame = 0

Query: 49  AFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDGDADVI 108
           A    +++   F + +    +S +N++  G     + D V  +  T      VD   DVI
Sbjct: 73  ALDQFIASLFTFLLLYILRRSSNKNKKNRGL-VVSQNDTVSKNLET-----EVDSGTDVI 132

Query: 109 IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEI 168
           IVGAGVAGSALAHTLGK+GRRVHVIERD +E DRIVGELLQPGGYLKLIELGL+DCV++I
Sbjct: 133 IVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKI 192

Query: 169 DAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVRLEQGT 228
           DAQ+V GY LFKDGK+T+L+YPLE F SDV+GRSFHNGRF+QRMR+KA +L NVRLEQGT
Sbjct: 193 DAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGT 252

Query: 229 VTSLLEKKGTIKG------------------------------------VDVPSCFVGLV 288
           VTSLLE+ GTIKG                                    VDVPS FVGLV
Sbjct: 253 VTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLV 312

Query: 289 LENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVA 348
           LENC+LPFANHGHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM KYLKT VA
Sbjct: 313 LENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVA 372

Query: 349 PQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVAL 408
           PQVP ++ +AFI A+EKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL
Sbjct: 373 PQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVAL 432

Query: 409 SDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASPDQARK 468
           +DIVVLR+L++P+++LND   L KY+ESFYTLR PVASTINTLA ALYKVF AS D+AR 
Sbjct: 433 ADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLASSDEART 492

Query: 469 EMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIW 528
           EMR+ACFDYLSLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LPFPS++  W
Sbjct: 493 EMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFW 552

Query: 529 IGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 532
           +GAR+I SAS IIFPIIKAEGVRQMFFP T+PA YR PP
Sbjct: 553 LGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of Clc08G17320 vs. ExPASy TrEMBL
Match: A0A0A0LKI1 (Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.8e-262
Identity = 462/526 (87.83%), Postives = 474/526 (90.11%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVDHC FGWI SAFLAF IAFSFFL+ R+NRR  G +STPR DC+ SSA+TNGECRSVDG
Sbjct: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMR+KAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTI G                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF 534
            KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF 526

BLAST of Clc08G17320 vs. ExPASy TrEMBL
Match: A0A6J1K5U9 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 8.1e-262
Identity = 462/530 (87.17%), Postives = 480/530 (90.57%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVD+CAFGWIL+A L  AIA SFF++ RRNRR GGADSTPR++ VRSS++TNGECRSVDG
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRSSSTTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNT+LSYPLE F SDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTIKG                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFKPIA 538
            KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV K IA
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA 529

BLAST of Clc08G17320 vs. ExPASy TrEMBL
Match: A0A5A7V0X5 (Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001090 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.4e-261
Identity = 463/525 (88.19%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVDHCAFGWI SA LAF IA S FL+  RNRRV G DSTPR+  V SSA+TNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTI G                                    VDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSF 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 533
           LKGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of Clc08G17320 vs. ExPASy TrEMBL
Match: A0A1S3BB88 (Squalene monooxygenase OS=Cucumis melo OX=3656 GN=LOC103487810 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.4e-261
Identity = 463/525 (88.19%), Postives = 473/525 (90.10%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVDHCAFGWI SA LAF IA S FL+  RNRRV G DSTPR+  V SSA+TNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTI G                                    VDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSY 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 533
           LKGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of Clc08G17320 vs. ExPASy TrEMBL
Match: A0A6J1HEG7 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 5.3e-261
Identity = 462/530 (87.17%), Postives = 479/530 (90.38%), Query Frame = 0

Query: 44  MVDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDG 103
           MVD+CAFGWIL+A L  AIA SFF++ RRNRR GGADSTPR++ VRSS++TNGECRSVD 
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRSSSTTNGECRSVDC 60

Query: 104 DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 163
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 164 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVR 223
           CVEEIDAQKVYGYALFKDGKNT+LSYPLE F SDVSGRSFHNGRFIQRMR+KAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 224 LEQGTVTSLLEKKGTIKG------------------------------------VDVPSC 283
           LEQGTVTSLLE+KGTIKG                                    VDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 284 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 343
           FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 344 KTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 403
           KT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 404 MTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASP 463
           MTVALSDIVVLRNL+KPLKDLNDAPTLCKYLESFYTLR PVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 464 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 523
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 524 LKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFKPIA 538
            KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV K IA
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Clc08G17320 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 716.1 bits (1847), Expect = 2.1e-206
Identity = 367/525 (69.90%), Postives = 421/525 (80.19%), Query Frame = 0

Query: 50  FGWIL-----SAFLAFAIAFSFFLASRRN--RRVGGADSTPRTDCVRSSASTNGECRSVD 109
           + WIL     S  ++F   + FF+  +RN  R      ST  +D    S +  G     D
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDV--GSVNITG-----D 65

Query: 110 GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 169
             ADVI+VGAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGELLQPGGYLKL+ELG++
Sbjct: 66  TVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIE 125

Query: 170 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNV 229
           DCVEEIDAQ+VYGYALFK+GK  RL+YPLE FH DVSGRSFHNGRFIQRMR+KAASLPNV
Sbjct: 126 DCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNV 185

Query: 230 RLEQGTVTSLLEKKGTIKG------------------------------------VDVPS 289
           +LEQGTV SLLE+ GTIKG                                    V+VPS
Sbjct: 186 QLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPS 245

Query: 290 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 349
           CFVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ Y
Sbjct: 246 CFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNY 305

Query: 350 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 409
           LKTVVAPQ+P E++D+FIAA++KGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGG
Sbjct: 306 LKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGG 365

Query: 410 GMTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCAS 469
           GMTVAL+DIVVLRNL++PL+DL+D  +LCKYLESFYTLR PVA+TINTLA ALY+VFC+S
Sbjct: 366 GMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSS 425

Query: 470 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 529
            ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFP
Sbjct: 426 ENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFP 485

Query: 530 SLKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 532
           S K IW+GA+LI  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 486 SPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of Clc08G17320 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 707.2 bits (1824), Expect = 1.0e-203
Identity = 359/523 (68.64%), Postives = 413/523 (78.97%), Query Frame = 0

Query: 45  VDHCAFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDGD 104
           VDHC    + + F+A   AF      RR  +        R   +   + T         D
Sbjct: 7   VDHCI---LTTTFVASLFAFLLLYVLRRRSKTIHGSVNVRNGTLTVKSGT---------D 66

Query: 105 ADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 164
            D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL+DC
Sbjct: 67  VDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDC 126

Query: 165 VEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVRL 224
           V++IDAQ+V GYALFKDGK+T+LSYPL+ F SDV+GRSFHNGRF+QRMR+KA+ LPNVR+
Sbjct: 127 VKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVRM 186

Query: 225 EQGTVTSLLEKKGTIKG------------------------------------VDVPSCF 284
           EQGTVTSL+E+ G IKG                                    V+VPS F
Sbjct: 187 EQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSNF 246

Query: 285 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 344
           VGLVLENC+LPF NHGHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+++GEM  +LK
Sbjct: 247 VGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHLK 306

Query: 345 TVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 404
           T+VAPQVPP+I DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGGGM
Sbjct: 307 TMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGGM 366

Query: 405 TVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASPD 464
           TVALSDIV+LR+L+ PL DL +  +L KY+ESFYTLR PVASTINTLAGALYKVF ASPD
Sbjct: 367 TVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASPD 426

Query: 465 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSL 524
            AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLVLHFFAVAI+GVGRLL+P PS+
Sbjct: 427 DARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPSV 486

Query: 525 KGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 532
           K +W+GARLI SASGIIFPIIKAEGVRQMFFP T+PA YR PP
Sbjct: 487 KRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of Clc08G17320 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 699.1 bits (1803), Expect = 2.7e-201
Identity = 356/519 (68.59%), Postives = 410/519 (79.00%), Query Frame = 0

Query: 49  AFGWILSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDGDADVI 108
           A    +++   F + +    +S +N++  G     + D V  +  T      VD   DVI
Sbjct: 73  ALDQFIASLFTFLLLYILRRSSNKNKKNRGL-VVSQNDTVSKNLET-----EVDSGTDVI 132

Query: 109 IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEI 168
           IVGAGVAGSALAHTLGK+GRRVHVIERD +E DRIVGELLQPGGYLKLIELGL+DCV++I
Sbjct: 133 IVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKI 192

Query: 169 DAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMRKKAASLPNVRLEQGT 228
           DAQ+V GY LFKDGK+T+L+YPLE F SDV+GRSFHNGRF+QRMR+KA +L NVRLEQGT
Sbjct: 193 DAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGT 252

Query: 229 VTSLLEKKGTIKG------------------------------------VDVPSCFVGLV 288
           VTSLLE+ GTIKG                                    VDVPS FVGLV
Sbjct: 253 VTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLV 312

Query: 289 LENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVA 348
           LENC+LPFANHGHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM KYLKT VA
Sbjct: 313 LENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVA 372

Query: 349 PQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVAL 408
           PQVP ++ +AFI A+EKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL
Sbjct: 373 PQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVAL 432

Query: 409 SDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASPDQARK 468
           +DIVVLR+L++P+++LND   L KY+ESFYTLR PVASTINTLA ALYKVF AS D+AR 
Sbjct: 433 ADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLASSDEART 492

Query: 469 EMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIW 528
           EMR+ACFDYLSLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LPFPS++  W
Sbjct: 493 EMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFW 552

Query: 529 IGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 532
           +GAR+I SAS IIFPIIKAEGVRQMFFP T+PA YR PP
Sbjct: 553 LGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of Clc08G17320 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 434.1 bits (1115), Expect = 1.6e-121
Identity = 233/515 (45.24%), Postives = 317/515 (61.55%), Query Frame = 0

Query: 54  LSAFLAFAIAFSFFLASRRNRRVGGADSTPRTDCVRSSASTNGECRSVDGDADVIIVGAG 113
           L   LAF + ++ F  + R     G  +T   D V             DG  DVIIVGAG
Sbjct: 8   LWTLLAFMLTWTVFYVTNR-----GKKATQLADAVVEERE--------DGATDVIIVGAG 67

Query: 114 VAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKV 173
           V GSALA+ L KDGRRVHVIERDL EP+RI+GE +QPGG L L +LGL+DC+E IDAQK 
Sbjct: 68  VGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSKLGLEDCLEGIDAQKA 127

Query: 174 YGYALFKDGKNTRLSYPLE--NFHSDVSGRSFHNGRFIQRMRKKAASLPNVRLEQGTVTS 233
            G  ++KDGK    S+P++  NF  D S RSFHNGRF+QR+R+KA+SLPNVRLE+GTV S
Sbjct: 128 TGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKASSLPNVRLEEGTVKS 187

Query: 234 LLEKKGTIKGV------------------------------------DVPSCFVGLVLEN 293
           L+E+KG IKGV                                    +V S  VG + +N
Sbjct: 188 LIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDNNAEVLSYQVGFISKN 247

Query: 294 CQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQV 353
           CQL       +++  PS  + Y ISST++RC+ +V    +PSISNGEM  ++K  +APQV
Sbjct: 248 CQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISNGEMATFVKNTIAPQV 307

Query: 354 PPEIHDAFIAAIEKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSD 413
           P ++   F+  I++G +I+ MP + M A      G +L+GDAFNMRHP    GM V LSD
Sbjct: 308 PLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNMRHPAIASGMMVLLSD 367

Query: 414 IVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYKVFCASPDQARKEM 473
           I++LR L++PL +L +A  + + ++SFY +R P+++T+NTL  A  +V  AS D+A++ M
Sbjct: 368 ILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQVLVASTDEAKEAM 427

Query: 474 RQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIWIG 530
           RQ C+DYLS GG  ++G ++LL G+NPRP+SL+ H  A+ +  +G LL PFPS   IW  
Sbjct: 428 RQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLLSPFPSPLRIWHS 487

BLAST of Clc08G17320 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 426.0 bits (1094), Expect = 4.4e-119
Identity = 216/468 (46.15%), Postives = 302/468 (64.53%), Query Frame = 0

Query: 102 DGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 161
           DG ADVIIVGAGV GSALA+ L KDGRRVHVIERD+ EP+R++GE +QPGG L L +LGL
Sbjct: 43  DGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSKLGL 102

Query: 162 QDCVEEIDAQKVYGYALFKDGKNTRLSYPLE--NFHSDVSGRSFHNGRFIQRMRKKAASL 221
           QDC+E+IDAQK  G A++KDGK     +P++  NF  + S RSFHNGRF+Q++R+KA SL
Sbjct: 103 QDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKAFSL 162

Query: 222 PNVRLEQGTVTSLLEKKGTIKGV------------------------------------- 281
            NVRLE+GTV SLLE+KG +KGV                                     
Sbjct: 163 SNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDDNNA 222

Query: 282 DVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGE 341
           ++ S  VG + +NC+L      H++L  PS  + Y ISST++RC  +V  +  PSI+NGE
Sbjct: 223 EIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIANGE 282

Query: 342 MEKYLKTVVAPQVPPEIHDAFIAAIEKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRH 401
           M  ++K  + PQVPP++   F+  I++G +I+ +P + M +      G +++GDAFNMRH
Sbjct: 283 MSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRH 342

Query: 402 PLTGGGMTVALSDIVVLRNLIKPLKDLNDAPTLCKYLESFYTLRMPVASTINTLAGALYK 461
           P+   GM V LSDI++LR L++PL +L DA  + + + SFY +R P+++T+NTL  A  +
Sbjct: 343 PVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQ 402

Query: 462 VFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRL 521
           V   S D+A++ MRQ  +DYL  GG  ++G ++LL G+NPRPLSLV H  A+ +  +G+L
Sbjct: 403 VLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQL 462

Query: 522 LLPFPSLKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRT 530
           L PFPS   IW   +L   A  ++ P +KAEGV QM FPA   AY+++
Sbjct: 463 LSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886247.12.4e-26889.04squalene monooxygenase SE1-like [Benincasa hispida][more]
XP_004142907.19.8e-26287.83squalene monooxygenase SE1 [Cucumis sativus] >KGN62420.1 hypothetical protein Cs... [more]
KAG6598316.19.8e-26287.36Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_022996831.11.7e-26187.17squalene monooxygenase-like isoform X1 [Cucurbita maxima][more]
XP_008444517.12.9e-26188.19PREDICTED: squalene monooxygenase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
B7TWW52.6e-20970.45Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2[more]
O486511.3e-20867.93Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1[more]
Q9SM023.0e-20569.90Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1[more]
Q8VYH21.4e-20268.64Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1[more]
O810003.8e-20068.59Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0LKI14.8e-26287.83Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1[more]
A0A6J1K5U98.1e-26287.17Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1[more]
A0A5A7V0X51.4e-26188.19Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
A0A1S3BB881.4e-26188.19Squalene monooxygenase OS=Cucumis melo OX=3656 GN=LOC103487810 PE=3 SV=1[more]
A0A6J1HEG75.3e-26187.17Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G58440.12.1e-20669.90FAD/NAD(P)-binding oxidoreductase family protein [more]
AT4G37760.11.0e-20368.64squalene epoxidase 3 [more]
AT2G22830.12.7e-20168.59squalene epoxidase 2 [more]
AT5G24150.11.6e-12145.24FAD/NAD(P)-binding oxidoreductase family protein [more]
AT5G24160.14.4e-11946.15squalene monoxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00420RNGMNOXGNASEcoord: 106..128
score: 43.04
coord: 347..362
score: 30.83
coord: 362..378
score: 32.55
NoneNo IPR availablePANTHERPTHR10835:SF17SQUALENE MONOOXYGENASE-RELATEDcoord: 242..532
NoneNo IPR availablePANTHERPTHR10835:SF17SQUALENE MONOOXYGENASE-RELATEDcoord: 44..243
IPR002938FAD-binding domainPFAMPF01494FAD_binding_3coord: 105..178
e-value: 4.1E-6
score: 26.3
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 293..432
e-value: 6.3E-8
score: 34.5
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 100..246
e-value: 6.4E-18
score: 66.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 105..444
IPR013698Squalene epoxidasePFAMPF08491SEcoord: 240..489
e-value: 4.7E-106
score: 353.9
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 242..532
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 44..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc08G17320.2Clc08G17320.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0071949 FAD binding
molecular_function GO:0004506 squalene monooxygenase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding