Clc05G22220 (gene) Watermelon (cordophanus) v2

Overview
NameClc05G22220
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationClcChr05: 30549458 .. 30552758 (-)
RNA-Seq ExpressionClc05G22220
SyntenyClc05G22220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAAACCCCCCGTCCTTGTTCCCCATTTTTTCCTTCCAAGGAGATTGATCGTAGCAAAAGAAGTATGGCAGTGAAGCAGAAAGAAGCCATGAACTTCAAAACTCAATGGGCTATTTTGGTCTCCTGTTGGACGCAGAAATTCTTTCTGGCCCTCGTCGTCCCTGTGCTCAAAATCTTAGGCACAATCCTAGGTATATATTCATATCACGTACGTTTCATAACTATTATGCCTTTCTTTCTTTTTCTCTTTCCAAATTACTAGTGTTGTGTGTTGCAGGAAAGCTGCGTATGAGGCCGCAGGCCCGGATTACTCCCATGAAATCATTGATTGTTAGACCGGAAGAAAGATATAAAGAGTGGAAAATAATAGTTCCGGACGACAAAAGCATGAGCGAAGAGGAGCGTGAGTTCAGGTATTATAGTGCCTTAACCATGATGGCTTCCAAATTGGCATATCAACACCATCTATTTGTCAACTTCGTCGCAACCTACTGTTGGAAGGTAACAAAAAGAAGTTCAACTTTTTGCATCTTCTTCGTATAATAACTAAATTCAATTTACTCTAGTAATCTATAAACTTTAAGCTTTTGAATATTTCATGGATGCTCTAATTCTTTCTTCCTATATATATATATATATATATATAACAGCTCTTATTCAACCCCTATTCTGTGCAAGAATTACCTGCGTTTCTTTAGTCTTTTTCTATTTTTATAAGTGAATAATGTGTGTGCAAGTAAAATTTGCAATATTATAAACTTCCGATAATGGGCTATTTTATTATGTTTAAAAAATTATTTTAAGAGACAAAATTATTAAAAATATTTATAAATAAATAGTAAAATTTTACTATAATTATAAATATTTTGGTTCACCTTGCTATATTTGAAATCAAAAAGAAAAAAGAAAAACATATCTAACATAATGAAGGCAACCGTTATATAATTTTGTAGCCATTTTTGTTGAGAAATTTATGAGGAAAATGATAGACAGCAGATGGATGATGTTTTTTCTTTGCTTTTCAAATGCAGATGGATCACGTGGAATGCTACAATTTTCAGAACCGTAAGACTTTTTTTTTTTTTTTTCTTCTTCTTCTTTCCCTTTTTTAGTTCAGATTTGAATAAAGAATAAATAAAGACTTTCGACTTGTCTTCTTGGAATAAATAAAAGATCTGGAATTAAAATATTTTTCAAATAAATAAAGACTTAGTCTAGTAGTTAAAGGTCATAATCGGAATAGATTTGACTTTTAAGATTTAATGGTATTTTGATTAATTAATTATTTTAAATAACAAAATTTTTGGAAATATGTTTAAATATAGAACAAAATAATAAAAAAACTTTTTCAAATTGACAACTAGTGATACATAATGGTAGACTTCTATTAGTGTCTATCACTAATAGATCACATCTTTCTATTATTGTCTATCTATTTGTGTCTATTATTGATGGACAAATTAATAGTGAACGATCACTTCTATGATCGCTCTTAACTGGACGACCTTTCTTTTAACTTCTCTGCTTCATTTTATATTCAAGCTTATCTCTAGCTCGTTCCGTATTTTTTGATGTTCGCCGTTTGAATGAATATACATCTCTCTTTGCCTTGAGAGGATGATGAAGATACTAAAGATGTATTAACCCTAGCTGAGATATATGGGTGCATTTACTGATCCCATTTCCGTTGGTATTTTGCTAAAAAAAAAAGGTTGAATTCTTGGTCAAATTCCAAAAACAAAAACTATTTATTTTTTGTTTTTAAATTTTTGATTGGTTTTTTAAAATGATAGCAAAAAAATAGATTAAAAAAAGGTAAAAAAAATTTAGAAGTGGAAGAGTTGTTTATAAGTTTAATTTTTAAAAATAAGAAAAAAAATATTTACTAAACAAAACTTATCCCGATTTGGAAACAATTATTTGCCTCAAACAAAAGCTTATTTATTATTTTTTTTTCACTTTACAACATTTAATTGGGTAGAATTTTCTCATTCCCCCACTGGAAACTAAACTATATATATCTCTTTTATTTTGAACTTGTTCAGCCTATTTGAAAACAAAATCGACTCATGCATTTACGTTTCAAAATACATCCAAAGACGTAACAGTGGTGGCATTTAGAGGGACCTCCGTGTTTGACCTTAACGACTGGATGGTAGATTCCAACTTTCTTTTTAAAAAATTAGATGGTGTTGGCCAAATCCATAGTGGGTTCATGGACGCCATCGGCTACCAAGATCAGCCAATCGCCCTCCATGAATGCCATGACAAGTTCGCATACCACACCCTTTGCCAAGAACTTAAAGAGATCGCCAAATCCAACGAAAATGCAAAATTTATCATCACTGGCCACAGTTTGGGTGGAGCCCTTGCCACCCTTTTTGTCACTTTGCTAGCCTATTACAAAGAAGCAACCATTCTTGACAAACTTCAAGCTGTTTATACGTATGGCCAGCCCAGAGTTGGGGACGACGAATTTGCACACTTTATGGTAGACACTTTCGATAATCACCACATTAAATATTACAGATATGTTTACTCTTTTGACATGATACCTAGAGTGCCTTTCGATGCTATGCTTACCAACTACAAACACTTTGGTGGATGTGTCTACTTTGATTGCTTCTACAACGGCAAGGTAACAATATCCATATATCCATAACTCTCTCTTTCTCCACATTAAAAACAATAGGCATATGTCCTTTGATTCAATATATAAATTTCATTCTCTTAGGCTTCCACTATTTAATACTATTATTGAAGACAATGAATAATTTTTTTGGGTGTTTTCATTGTATGCAGTTTCTAAAGGAGCAGCCAAACACAAACTACTTCTCGCTAATTTGGGTAATACCCAAGTACTTAATTGCATGTTGGGAGCTTATTAGAAGTTTAATTATAATCCCAATTACCAAGGGTAGGGATCACTTCGAGGGTCTTTTTACGATATTGGAAAGGATCCTTGGGTTGCTTATTCCAGGATTATCAGCTCACGTATGTTCAAATTATGTTAACTTGACTCGTTGGGGGACAATACAATTGCCACCCGACCACAACCCAATCTTGAGGAACTTTGCTCGCCATATCGAAGCCGACTACTAAGAATTGTATTGAGTTGATGAGTATGTTTGAATTAACTTTTTAAATACTTATGATTTTTTTTCCTTTTTTTTTTCTTCAAAATAATTAAAAAGTAAATCTAAATAGGCTCTAAACAGCAATTTACTTCTTTTAAATTTTGGATTTGTGAAAGTTTGGAATTAGAATACATGATTATAATTCCAAATAAATGTTTTGACT

mRNA sequence

ATAAAACCCCCCGTCCTTGTTCCCCATTTTTTCCTTCCAAGGAGATTGATCGTAGCAAAAGAAGTATGGCAGTGAAGCAGAAAGAAGCCATGAACTTCAAAACTCAATGGGCTATTTTGGTCTCCTGTTGGACGCAGAAATTCTTTCTGGCCCTCGTCGTCCCTGTGCTCAAAATCTTAGGCACAATCCTAGGAAAGCTGCGTATGAGGCCGCAGGCCCGGATTACTCCCATGAAATCATTGATTGTTAGACCGGAAGAAAGATATAAAGAGTGGAAAATAATAGTTCCGGACGACAAAAGCATGAGCGAAGAGGAGCGTGAGTTCAGGTATTATAGTGCCTTAACCATGATGGCTTCCAAATTGGCATATCAACACCATCTATTTGTCAACTTCGTCGCAACCTACTGTTGGAAGATGGATCACGTGGAATGCTACAATTTTCAGAACCCCTATTTGAAAACAAAATCGACTCATGCATTTACGTTTCAAAATACATCCAAAGACGTAACAGTGGTGGCATTTAGAGGGACCTCCGTGTTTGACCTTAACGACTGGATGGTAGATTCCAACTTTCTTTTTAAAAAATTAGATGGTGTTGGCCAAATCCATAGTGGGTTCATGGACGCCATCGGCTACCAAGATCAGCCAATCGCCCTCCATGAATGCCATGACAAGTTCGCATACCACACCCTTTGCCAAGAACTTAAAGAGATCGCCAAATCCAACGAAAATGCAAAATTTATCATCACTGGCCACAGTTTGGGTGGAGCCCTTGCCACCCTTTTTGTCACTTTGCTAGCCTATTACAAAGAAGCAACCATTCTTGACAAACTTCAAGCTGTTTATACGTATGGCCAGCCCAGAGTTGGGGACGACGAATTTGCACACTTTATGGTAGACACTTTCGATAATCACCACATTAAATATTACAGATATGTTTACTCTTTTGACATGATACCTAGAGTGCCTTTCGATGCTATGCTTACCAACTACAAACACTTTGGTGGATGTGTCTACTTTGATTGCTTCTACAACGGCAAGTTTCTAAAGGAGCAGCCAAACACAAACTACTTCTCGCTAATTTGGGTAATACCCAAGTACTTAATTGCATGTTGGGAGCTTATTAGAAGTTTAATTATAATCCCAATTACCAAGGGTAGGGATCACTTCGAGGGTCTTTTTACGATATTGGAAAGGATCCTTGGGTTGCTTATTCCAGGATTATCAGCTCACGTATGTTCAAATTATGTTAACTTGACTCGTTGGGGGACAATACAATTGCCACCCGACCACAACCCAATCTTGAGGAACTTTGCTCGCCATATCGAAGCCGACTACTAAGAATTGTATTGAGTTGATGAGTATGTTTGAATTAACTTTTTAAATACTTATGATTTTTTTTCCTTTTTTTTTTCTTCAAAATAATTAAAAAGTAAATCTAAATAGGCTCTAAACAGCAATTTACTTCTTTTAAATTTTGGATTTGTGAAAGTTTGGAATTAGAATACATGATTATAATTCCAAATAAATGTTTTGACT

Coding sequence (CDS)

ATGGCAGTGAAGCAGAAAGAAGCCATGAACTTCAAAACTCAATGGGCTATTTTGGTCTCCTGTTGGACGCAGAAATTCTTTCTGGCCCTCGTCGTCCCTGTGCTCAAAATCTTAGGCACAATCCTAGGAAAGCTGCGTATGAGGCCGCAGGCCCGGATTACTCCCATGAAATCATTGATTGTTAGACCGGAAGAAAGATATAAAGAGTGGAAAATAATAGTTCCGGACGACAAAAGCATGAGCGAAGAGGAGCGTGAGTTCAGGTATTATAGTGCCTTAACCATGATGGCTTCCAAATTGGCATATCAACACCATCTATTTGTCAACTTCGTCGCAACCTACTGTTGGAAGATGGATCACGTGGAATGCTACAATTTTCAGAACCCCTATTTGAAAACAAAATCGACTCATGCATTTACGTTTCAAAATACATCCAAAGACGTAACAGTGGTGGCATTTAGAGGGACCTCCGTGTTTGACCTTAACGACTGGATGGTAGATTCCAACTTTCTTTTTAAAAAATTAGATGGTGTTGGCCAAATCCATAGTGGGTTCATGGACGCCATCGGCTACCAAGATCAGCCAATCGCCCTCCATGAATGCCATGACAAGTTCGCATACCACACCCTTTGCCAAGAACTTAAAGAGATCGCCAAATCCAACGAAAATGCAAAATTTATCATCACTGGCCACAGTTTGGGTGGAGCCCTTGCCACCCTTTTTGTCACTTTGCTAGCCTATTACAAAGAAGCAACCATTCTTGACAAACTTCAAGCTGTTTATACGTATGGCCAGCCCAGAGTTGGGGACGACGAATTTGCACACTTTATGGTAGACACTTTCGATAATCACCACATTAAATATTACAGATATGTTTACTCTTTTGACATGATACCTAGAGTGCCTTTCGATGCTATGCTTACCAACTACAAACACTTTGGTGGATGTGTCTACTTTGATTGCTTCTACAACGGCAAGTTTCTAAAGGAGCAGCCAAACACAAACTACTTCTCGCTAATTTGGGTAATACCCAAGTACTTAATTGCATGTTGGGAGCTTATTAGAAGTTTAATTATAATCCCAATTACCAAGGGTAGGGATCACTTCGAGGGTCTTTTTACGATATTGGAAAGGATCCTTGGGTTGCTTATTCCAGGATTATCAGCTCACGTATGTTCAAATTATGTTAACTTGACTCGTTGGGGGACAATACAATTGCCACCCGACCACAACCCAATCTTGAGGAACTTTGCTCGCCATATCGAAGCCGACTACTAA

Protein sequence

MAVKQKEAMNFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRPQARITPMKSLIVRPEERYKEWKIIVPDDKSMSEEEREFRYYSALTMMASKLAYQHHLFVNFVATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKDVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMDAIGYQDQPIALHECHDKFAYHTLCQELKEIAKSNENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPDHNPILRNFARHIEADY
Homology
BLAST of Clc05G22220 vs. NCBI nr
Match: XP_031739748.1 (uncharacterized protein LOC105435389 [Cucumis sativus])

HSP 1 Score: 501.5 bits (1290), Expect = 7.1e-138
Identity = 259/436 (59.40%), Postives = 318/436 (72.94%), Query Frame = 0

Query: 10  NFKTQWAILVSCWTQKFFLA------LVVPVLKILGTILGKLRMRPQARITPMKSL---I 69
           NFK +W I VS  TQKF  A      ++V    I+     K  +  +  + P + +    
Sbjct: 64  NFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTD 123

Query: 70  VRPEERYKEWKIIVPDDKS-MSEEEREFRYYSALTMMASKLAYQHH----LFVNFVATYC 129
           V+P  R  +W+++VPDD + M+  +R+F+YYSALT+MASKLAYQ +      V FV   C
Sbjct: 124 VQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDC 183

Query: 130 WKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFK 189
           W+M  ++C NF N +    +THA  F+NT KD  VTV+AFRGTSV D+NDWMVD +F + 
Sbjct: 184 WQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWF 243

Query: 190 KLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITG 249
            L+G   IHSGFM A+GYQ     P  L +   +FAY+ L Q L+EIAKSN+NAKFIITG
Sbjct: 244 LLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITG 303

Query: 250 HSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYR 309
           HSLGGALATLFVTLLAY+ E  +LDK+QAVYT+GQPRVG+  FA FMVDTF  H IKYYR
Sbjct: 304 HSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYR 363

Query: 310 YVYSFDMIPRVPFDAMLT-NYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIA 369
           YVYSFD++PR+PF ++   +Y+HFGGCVYFD FYNGKFLKEQPNTNYFSLIWVIPKYL A
Sbjct: 364 YVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSA 423

Query: 370 CWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPD 426
            WE IRSLII PI KGR +FEG FTI+ER +GL+IPG+SAHVCSNYVNLTRWG I LPPD
Sbjct: 424 DWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPD 483

BLAST of Clc05G22220 vs. NCBI nr
Match: KGN55200.1 (hypothetical protein Csa_012841 [Cucumis sativus])

HSP 1 Score: 501.5 bits (1290), Expect = 7.1e-138
Identity = 259/436 (59.40%), Postives = 318/436 (72.94%), Query Frame = 0

Query: 10  NFKTQWAILVSCWTQKFFLA------LVVPVLKILGTILGKLRMRPQARITPMKSL---I 69
           NFK +W I VS  TQKF  A      ++V    I+     K  +  +  + P + +    
Sbjct: 53  NFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTD 112

Query: 70  VRPEERYKEWKIIVPDDKS-MSEEEREFRYYSALTMMASKLAYQHH----LFVNFVATYC 129
           V+P  R  +W+++VPDD + M+  +R+F+YYSALT+MASKLAYQ +      V FV   C
Sbjct: 113 VQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDC 172

Query: 130 WKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFK 189
           W+M  ++C NF N +    +THA  F+NT KD  VTV+AFRGTSV D+NDWMVD +F + 
Sbjct: 173 WQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWF 232

Query: 190 KLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITG 249
            L+G   IHSGFM A+GYQ     P  L +   +FAY+ L Q L+EIAKSN+NAKFIITG
Sbjct: 233 LLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITG 292

Query: 250 HSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYR 309
           HSLGGALATLFVTLLAY+ E  +LDK+QAVYT+GQPRVG+  FA FMVDTF  H IKYYR
Sbjct: 293 HSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYR 352

Query: 310 YVYSFDMIPRVPFDAMLT-NYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIA 369
           YVYSFD++PR+PF ++   +Y+HFGGCVYFD FYNGKFLKEQPNTNYFSLIWVIPKYL A
Sbjct: 353 YVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSA 412

Query: 370 CWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPD 426
            WE IRSLII PI KGR +FEG FTI+ER +GL+IPG+SAHVCSNYVNLTRWG I LPPD
Sbjct: 413 DWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPD 472

BLAST of Clc05G22220 vs. NCBI nr
Match: XP_038896694.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 499.2 bits (1284), Expect = 3.5e-137
Identity = 259/439 (59.00%), Postives = 325/439 (74.03%), Query Frame = 0

Query: 10  NFKTQWAILVSCWTQKFFLALVVPVLKILGT-------------ILGKLRMRPQARITPM 69
           NFK +W I VS   QKF L L   +L +L               +L +  + PQ     +
Sbjct: 53  NFKARWTISVSILAQKFLLTLAT-LLTLLAAFWSIIQRFFYKYILLAEFWVAPQT----I 112

Query: 70  KSLIVRPEERYKEWKIIVPDDKS-MSEEEREFRYYSALTMMASKLAYQHH----LFVNFV 129
             + ++P+ R ++WKI+ P+D + M+ EER+FRYYS LT+MASKLAYQ +      V  V
Sbjct: 113 NFMDLKPKVRCRDWKILDPNDNADMNLEERDFRYYSNLTIMASKLAYQDYSQYPSVVESV 172

Query: 130 ATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSN 189
              CW+M+ ++C+NF N +    +THAF F+NTSKD  VTVVAFRGTSV + +DW VD N
Sbjct: 173 VNDCWQMNLIDCFNFWNDFQDKATTHAFMFENTSKDPKVTVVAFRGTSVLEPSDWTVDFN 232

Query: 190 FLFKKLDGVGQIHSGFMDAIGYQD---QPIALHECHDKFAYHTLCQELKEIAKSNENAKF 249
           F + KL+GVG IH+GFM A+G+QD    P  L +   ++AY+TL Q+LK+IAK+N+NA+F
Sbjct: 233 FSWSKLEGVGNIHAGFMQALGFQDLAGWPKDLLQPKHQYAYYTLRQKLKDIAKANDNAEF 292

Query: 250 IITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHI 309
           IITGHSLGGALATLFVTLLA++ E TIL K++AVYTYGQPRVG+ EFA FMVDT + +  
Sbjct: 293 IITGHSLGGALATLFVTLLAHHNETTILHKIRAVYTYGQPRVGNQEFAQFMVDTVERYGF 352

Query: 310 KYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKY 369
           KYYRYVYSFD++PRVPFD+++  YKHFGGCVYFDCFY+GKFLKEQPNTNYFS IW+IPKY
Sbjct: 353 KYYRYVYSFDLVPRVPFDSVVFKYKHFGGCVYFDCFYHGKFLKEQPNTNYFSPIWLIPKY 412

Query: 370 LIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQL 426
           L ACWE IRSLII PI KGRD+FEG FTILER LGLLIPG+SAHVCSNYVN+TR G IQL
Sbjct: 413 LSACWEFIRSLIITPIVKGRDYFEGFFTILERTLGLLIPGISAHVCSNYVNVTRLGNIQL 472

BLAST of Clc05G22220 vs. NCBI nr
Match: XP_022936593.1 (uncharacterized protein LOC111443149 [Cucurbita moschata])

HSP 1 Score: 466.1 bits (1198), Expect = 3.3e-127
Identity = 244/434 (56.22%), Postives = 307/434 (70.74%), Query Frame = 0

Query: 4   KQKEAMNFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRPQARITPMKSLIVR- 63
           K++   +FK +  I VS   QKF LAL  P+L  L   L  ++      +        R 
Sbjct: 49  KEESYSSFKARCTIFVSILVQKFLLALAAPLL-TLRAFLSVIQPLFFNYMIGKGEFYTRS 108

Query: 64  ---PEERYKEWKIIVPDDKSMSEEEREFRYYSALTMMASKLAYQHHL----FVNFVATYC 123
              PE R ++W+I  PDDK        F+YY ALTMMAS+LAY+ +      V+ V   C
Sbjct: 109 DSGPEVRCRDWQIEDPDDKG-------FKYYGALTMMASQLAYEDYTASPSVVHSVVNGC 168

Query: 124 WKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFK 183
           W+M  + C+NF N +    +T AF FQNT+KD  VTVVAFRGTSVF+ +DWMVD N  + 
Sbjct: 169 WQMTLLGCFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWY 228

Query: 184 KLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITG 243
           K++G+G+IHSGFM A+G Q     P  L E   +FAY+TL + L++I KSN+ AKFIITG
Sbjct: 229 KIEGIGRIHSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITG 288

Query: 244 HSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYR 303
           HSLGGALATLFVTLLA ++++TIL +LQAVYT+GQPRVGD +FA FMV+T   + +KYYR
Sbjct: 289 HSLGGALATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYR 348

Query: 304 YVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIAC 363
           YVYSFD++PRVPFD+++  YKHFGGC+YFDCFYNGKF KEQPNTNYFSLIWVIPKY  A 
Sbjct: 349 YVYSFDLVPRVPFDSIIFKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAH 408

Query: 364 WELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPDH 423
           WELI SLIIIP+ KGR++FEG  T LER++GL  PGLSAH+C NYVN+TRWG I++ PD 
Sbjct: 409 WELISSLIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDR 468

Query: 424 NPILRNFARHIEAD 425
           N  L+   R+IEAD
Sbjct: 469 NETLK-LVRYIEAD 473

BLAST of Clc05G22220 vs. NCBI nr
Match: KAG6591383.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 464.2 bits (1193), Expect = 1.2e-126
Identity = 243/434 (55.99%), Postives = 306/434 (70.51%), Query Frame = 0

Query: 4   KQKEAMNFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRPQARITPMKSLIVR- 63
           K++   +FK +  I VS   QKF LAL  P+L  L   L  ++      +        R 
Sbjct: 49  KEESYSSFKARCTIFVSILVQKFLLALAAPLL-TLRAFLSVIQPLFFNYMIGKGEFYTRS 108

Query: 64  ---PEERYKEWKIIVPDDKSMSEEEREFRYYSALTMMASKLAYQHHL----FVNFVATYC 123
              PE R ++W+I  PDDK        F+YY ALTMMAS+LAY+ +      V+ V   C
Sbjct: 109 DSGPEVRCRDWQIEDPDDKG-------FKYYGALTMMASQLAYEDYTASPSVVHSVVNGC 168

Query: 124 WKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFK 183
           W+M  + C+NF N +    +T AF FQNT+KD  VTVVAFRGTSVF+ +DWMVD N  + 
Sbjct: 169 WQMTLLGCFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWY 228

Query: 184 KLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITG 243
           K++G+G+IHSGFM A+G Q     P  L     +FAY+TL Q L++I KSN+ AKFI+TG
Sbjct: 229 KIEGIGRIHSGFMQALGLQKATGWPKELPGAEHEFAYYTLRQRLRDIVKSNDKAKFIMTG 288

Query: 244 HSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYR 303
           HSLGGALATLFVTLLA ++++TIL +LQAVYT+GQPRVGD +FA FMV+T   +  KYYR
Sbjct: 289 HSLGGALATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGFKYYR 348

Query: 304 YVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIAC 363
           YVYSFD++PRVPFD+++  YKHFGGC+YFDCFYNGKF +EQPNTNYFSLIWVIPKYL A 
Sbjct: 349 YVYSFDLVPRVPFDSIIFKYKHFGGCLYFDCFYNGKFRREQPNTNYFSLIWVIPKYLSAH 408

Query: 364 WELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPDH 423
           WELI SLIIIP+ KGR++FEG  T LER++GL  PGLSAH+C NYVN+TRWG I++ PD 
Sbjct: 409 WELISSLIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDR 468

Query: 424 NPILRNFARHIEAD 425
           N  L+   R+IEAD
Sbjct: 469 NETLK-LVRYIEAD 473

BLAST of Clc05G22220 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.9e-61
Identity = 140/365 (38.36%), Postives = 204/365 (55.89%), Query Frame = 0

Query: 67  YKEWKI------IVPDDKSMSEEEREFRYYSALTMMASKLAYQHHLFVNFVATYCWKMDH 126
           YKEW        I   D    + E   R    L +MASKLAY++   V  V    WKM+ 
Sbjct: 145 YKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVVDLHWKMNL 204

Query: 127 VECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGV 186
           VE  +  N Y K  ST  F F +  KD  + V++FRGT  FD +DW  D ++ + ++  V
Sbjct: 205 VEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDYSWYEVPNV 264

Query: 187 GQIHSGFMDA--IGYQDQPIALH-------------------ECHDKFAYHTLCQELKEI 246
           G++H GF++A  +G +D     H                   +  ++ AY+ +   LK +
Sbjct: 265 GKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRL 324

Query: 247 AKSNENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFM 306
              +ENA+F++TGHSLGGALA LF TLL   +E  I+ +L  VYT+GQPR+G+ E   FM
Sbjct: 325 LSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFM 384

Query: 307 VDTFDNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYF 366
               +    +Y+R VY  D++PR+P+D     YKHFG C+++D FYN    +++P+ N +
Sbjct: 385 KAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPY 444

Query: 367 SLIWVIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVN 403
            L + I  ++IA WEL+R L  +  T G D+ EG F IL R++GL+IPGLS H  ++YVN
Sbjct: 445 GLRYKILGHVIAVWELVRGL-TMGYTHGPDYKEGWFRILFRLMGLVIPGLSDHCMTDYVN 504

BLAST of Clc05G22220 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.4e-56
Identity = 127/374 (33.96%), Postives = 197/374 (52.67%), Query Frame = 0

Query: 93  LTMMASKLAYQHHLFVNFVATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTV 152
           L MMASKLAY++   V  V    WKM  V+ YN  N + K  ST  F   +  KD  + +
Sbjct: 190 LCMMASKLAYENEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLIL 249

Query: 153 VAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMDAIGYQDQPIA------------- 212
           V+FRGT  FD +DW+ D ++ + ++  +G++H GF++A+G  ++  A             
Sbjct: 250 VSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLK 309

Query: 213 ------LH-------------------------------------------ECHDKFAYH 272
                 +H                                           E  ++ AY+
Sbjct: 310 FANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYY 369

Query: 273 TLCQELKEIAKSNENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRV 332
            +  +LK + K ++NAKF++TGHSLGGALA LF  +L  ++E  ++++L  +YTYGQPRV
Sbjct: 370 VVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRV 429

Query: 333 GDDEFAHFMVDTFDNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFL 392
           G+ +   FM    ++   KY+R VY  D++PR+P+D     +KHFG C Y++  Y  + +
Sbjct: 430 GNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNI 489

Query: 393 KEQPNTNYFSLIWVIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLS 403
            E+PN NYF + +++P YL A WELIRS   +    G ++ E   +++ R LGL +PG+S
Sbjct: 490 NEEPNPNYFGMRFLVPLYLNAGWELIRS-FTMGYMYGSEYEECWESVMLRALGLFLPGIS 549

BLAST of Clc05G22220 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.6e-55
Identity = 126/339 (37.17%), Postives = 184/339 (54.28%), Query Frame = 0

Query: 93  LTMMASKLAYQHHLFVNFVATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTV 152
           L +MASKLAY++   V  V    WKM  V  Y   N +   ++THAF F +  KD  + V
Sbjct: 172 LCIMASKLAYENTKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIV 231

Query: 153 VAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMDAIGY--------------QDQPI 212
           ++FRGT  F + +W  D +F    L   G +H GF++A+G                  P 
Sbjct: 232 ISFRGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPG 291

Query: 213 ALHECHDKF-------------AYHTLCQELKEIAKSNENAKFIITGHSLGGALATLFVT 272
           ++ E   +               Y      LK + K ++NAKF++TGHSLGGALA LF  
Sbjct: 292 SITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTC 351

Query: 273 LLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYRYVYSFDMIPRVPF 332
           +L   +E  +LD+L  VYT+GQPR+G+    +FM +  +    +Y+R VY  DM+PRVPF
Sbjct: 352 ILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPF 411

Query: 333 DAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIACWELIRSLIIIPIT 392
           D +   ++HFG C+Y+D  + G F KE+P+ N F +   I  ++ A WEL RS I+  + 
Sbjct: 412 DDVFFTFEHFGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHV- 471

Query: 393 KGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWG 403
            G ++ E   + + RILGL +PG++AH   NYVN  R G
Sbjct: 472 YGAEYKETWESRMFRILGLFLPGVAAHSPVNYVNSVRLG 509

BLAST of Clc05G22220 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-11
Identity = 54/171 (31.58%), Postives = 86/171 (50.29%), Query Frame = 0

Query: 136 THAFTFQNTSKDVTVVAFRGTSVFDLNDWMVDSNFL---FKKLDGVGQIHSGFMDAIG-Y 195
           T+A   +  S+    + FRG+S   + +W+ D  F+   +  + G  ++H GF+D+ G  
Sbjct: 156 TNAMVARGDSEKTIYIVFRGSS--SIRNWIADLTFVPVSYPPVSGT-KVHKGFLDSYGEV 215

Query: 196 QDQPIALHECHDKFAYHTLCQELKEIAKSNENAKFIITGHSLGGALATLFVTLLAYYKEA 255
           Q++ +A           T+  + K+      + K  +TGHSLGGA A L   L  Y +E 
Sbjct: 216 QNELVA-----------TVLDQFKQY----PSYKVAVTGHSLGGATA-LLCALDLYQREE 275

Query: 256 TILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYRYVYSFDMIPRVP 303
            +      +YT GQPRVGD  FA+++V T     I Y R V   D++P +P
Sbjct: 276 GLSSSNLFLYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP 303

BLAST of Clc05G22220 vs. ExPASy Swiss-Prot
Match: P61871 (Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 9.3e-08
Identity = 47/167 (28.14%), Postives = 82/167 (49.10%), Query Frame = 0

Query: 136 THAFTFQNTSKDVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMDAIGYQDQP 195
           T+ +  ++  +    + FRGT+ F      +  NF   K     ++H+GF+ +    +Q 
Sbjct: 186 TNGYVLRSDKQKTIYLVFRGTNSFRSAITDIVFNFSDYKPVKGAKVHAGFLSS---YEQV 245

Query: 196 IALHECHDKFAYHTLCQELKEIAKSNENAKFIITGHSLGGALATLFVTLLAYYKEATILD 255
           +     +D F        ++E   ++   K I+TGHSLGGA A L   +  Y +E  +  
Sbjct: 246 V-----NDYFPV------VQEQLTAHPTYKVIVTGHSLGGAQA-LLAGMDLYQREPRLSP 305

Query: 256 KLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYRYVYSFDMIPRVP 303
           K  +++T G PRVG+  FA+++  T     I + R V+  D++P VP
Sbjct: 306 KNLSIFTVGGPRVGNPTFAYYVEST----GIPFQRTVHKRDIVPHVP 333

BLAST of Clc05G22220 vs. ExPASy TrEMBL
Match: A0A0A0KZS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 3.4e-138
Identity = 259/436 (59.40%), Postives = 318/436 (72.94%), Query Frame = 0

Query: 10  NFKTQWAILVSCWTQKFFLA------LVVPVLKILGTILGKLRMRPQARITPMKSL---I 69
           NFK +W I VS  TQKF  A      ++V    I+     K  +  +  + P + +    
Sbjct: 53  NFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTD 112

Query: 70  VRPEERYKEWKIIVPDDKS-MSEEEREFRYYSALTMMASKLAYQHH----LFVNFVATYC 129
           V+P  R  +W+++VPDD + M+  +R+F+YYSALT+MASKLAYQ +      V FV   C
Sbjct: 113 VQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDC 172

Query: 130 WKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFK 189
           W+M  ++C NF N +    +THA  F+NT KD  VTV+AFRGTSV D+NDWMVD +F + 
Sbjct: 173 WQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWF 232

Query: 190 KLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITG 249
            L+G   IHSGFM A+GYQ     P  L +   +FAY+ L Q L+EIAKSN+NAKFIITG
Sbjct: 233 LLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITG 292

Query: 250 HSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYR 309
           HSLGGALATLFVTLLAY+ E  +LDK+QAVYT+GQPRVG+  FA FMVDTF  H IKYYR
Sbjct: 293 HSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYR 352

Query: 310 YVYSFDMIPRVPFDAMLT-NYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIA 369
           YVYSFD++PR+PF ++   +Y+HFGGCVYFD FYNGKFLKEQPNTNYFSLIWVIPKYL A
Sbjct: 353 YVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSA 412

Query: 370 CWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPD 426
            WE IRSLII PI KGR +FEG FTI+ER +GL+IPG+SAHVCSNYVNLTRWG I LPPD
Sbjct: 413 DWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPD 472

BLAST of Clc05G22220 vs. ExPASy TrEMBL
Match: A0A6J1FDN5 (uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC111443149 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.6e-127
Identity = 244/434 (56.22%), Postives = 307/434 (70.74%), Query Frame = 0

Query: 4   KQKEAMNFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRPQARITPMKSLIVR- 63
           K++   +FK +  I VS   QKF LAL  P+L  L   L  ++      +        R 
Sbjct: 49  KEESYSSFKARCTIFVSILVQKFLLALAAPLL-TLRAFLSVIQPLFFNYMIGKGEFYTRS 108

Query: 64  ---PEERYKEWKIIVPDDKSMSEEEREFRYYSALTMMASKLAYQHHL----FVNFVATYC 123
              PE R ++W+I  PDDK        F+YY ALTMMAS+LAY+ +      V+ V   C
Sbjct: 109 DSGPEVRCRDWQIEDPDDKG-------FKYYGALTMMASQLAYEDYTASPSVVHSVVNGC 168

Query: 124 WKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFK 183
           W+M  + C+NF N +    +T AF FQNT+KD  VTVVAFRGTSVF+ +DWMVD N  + 
Sbjct: 169 WQMTLLGCFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWY 228

Query: 184 KLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITG 243
           K++G+G+IHSGFM A+G Q     P  L E   +FAY+TL + L++I KSN+ AKFIITG
Sbjct: 229 KIEGIGRIHSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITG 288

Query: 244 HSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYR 303
           HSLGGALATLFVTLLA ++++TIL +LQAVYT+GQPRVGD +FA FMV+T   + +KYYR
Sbjct: 289 HSLGGALATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYR 348

Query: 304 YVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIAC 363
           YVYSFD++PRVPFD+++  YKHFGGC+YFDCFYNGKF KEQPNTNYFSLIWVIPKY  A 
Sbjct: 349 YVYSFDLVPRVPFDSIIFKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAH 408

Query: 364 WELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPPDH 423
           WELI SLIIIP+ KGR++FEG  T LER++GL  PGLSAH+C NYVN+TRWG I++ PD 
Sbjct: 409 WELISSLIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDR 468

Query: 424 NPILRNFARHIEAD 425
           N  L+   R+IEAD
Sbjct: 469 NETLK-LVRYIEAD 473

BLAST of Clc05G22220 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 3.7e-100
Identity = 204/410 (49.76%), Postives = 262/410 (63.90%), Query Frame = 0

Query: 4   KQKEAMNFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRPQARITPMKSLIVRP 63
           K+     F  +W I +S   QKF LA +  ++K   ++ GK      A           P
Sbjct: 47  KENSYTTFGNRWVIFISILLQKFLLA-IANLIKYCKSMRGKFHKTCAA-----------P 106

Query: 64  EERYKEWKIIVPDDKSMSEEEREFRYYSALTMMASKLAYQHH-----LFVNFVATYCWKM 123
           +   KEWKII  +      ++  F+YY ALT+MAS LAYQ +       V  V   CWKM
Sbjct: 107 KVNCKEWKIIGMEKNIKLADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKM 166

Query: 124 DHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLD 183
           + + CY+F N +    +T  F FQNT+ D  VTVVAFRG+S  ++ DWMVD N  +  ++
Sbjct: 167 NLLGCYDFWNDFQNKATTQVFMFQNTATDPNVTVVAFRGSS--EIYDWMVDLNLSWYNIE 226

Query: 184 GVGQIHSGFMDAIGYQ---DQPIALHECHDKFAYHTLCQELKEIAKSNENAKFIITGHSL 243
           G+G+IH GFM A+G Q     P  L      FAY+TL Q+L++I KSN+NA+FI TGHSL
Sbjct: 227 GIGKIHGGFMQALGLQKGTGWPKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGHSL 286

Query: 244 GGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYRYVY 303
           GGALA LF TLLA++ +AT+L+KLQAVYT+GQPRVGD  FA FM +T + +  KYYRYVY
Sbjct: 287 GGALAVLFATLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVY 346

Query: 304 SFDMIPRVPFDAMLTN-YKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIACWE 363
           S D++PRVPFD      Y+HFGGCVYF+C YNGKFL+ QPN NYFS IW+I KY+ A WE
Sbjct: 347 SSDLVPRVPFDFNDNLWYEHFGGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWE 406

Query: 364 LIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWG 403
           LIRSL+I  I    D+ EG   +L R+ GLL PG SAH+C NY+N  RWG
Sbjct: 407 LIRSLMIPVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWG 442

BLAST of Clc05G22220 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 214/440 (48.64%), Postives = 281/440 (63.86%), Query Frame = 0

Query: 10  NFKTQWAILVSCWTQKFFLALVVPVLKI-------------LGTILGKLRMRPQARITPM 69
           +F  +W I +S   Q   LA+  P+ K+              G ILG L     +RI   
Sbjct: 54  SFTNRWLIFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLL-----SRILKG 113

Query: 70  KSLIVRPEERYKEWKIIV-------PDDKSMSEEEREFRYYSALTMMASKLAYQHHLFVN 129
           +SL V+P+E   E+  +V         DKS+  EE  FRYY  LT+MA++L+Y+   FV 
Sbjct: 114 ESL-VKPDENSAEYTSVVGFADWRRDLDKSIKAEE-IFRYYGGLTVMAAQLSYESKPFVL 173

Query: 130 FVATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVD 189
            V    WKM  +  +NF N +    +T AF FQNT+ D  VTVVAFRGTS  D  DW VD
Sbjct: 174 SVVNDRWKMKLLGHFNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVD 233

Query: 190 SNFLFKKLDGVGQIHSGFMDAIGYQDQ---PIALHECHDKFAYHTLCQELKEIAKSNENA 249
           ++F +  ++GVG+IHSGFM A+G Q     P  L +    FAY+TL Q+L++IAKSN+NA
Sbjct: 234 ADFSWYDIEGVGRIHSGFMKALGLQKATGWPEELVKSKHHFAYYTLRQKLRDIAKSNDNA 293

Query: 250 KFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNH 309
           KFIITGHSLGGALA LFVT+L+ ++++ +L+KLQAVYTY QPRVGD  FA FMV+T   +
Sbjct: 294 KFIITGHSLGGALAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKY 353

Query: 310 HIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIP 369
             KY+RYVYS D++PRVPFD ++  YKHFG C+YF+  Y G+ +KE+PN NYFSLIWV+P
Sbjct: 354 GFKYHRYVYSSDLVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMP 413

Query: 370 KYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTI 425
           KYL A WELIRS  I+P+ KG D+ E L     R++GL IPGL+AH+ ++YVN  R G  
Sbjct: 414 KYLNAWWELIRS-FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKF 473

BLAST of Clc05G22220 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 2.6e-98
Identity = 210/428 (49.07%), Postives = 278/428 (64.95%), Query Frame = 0

Query: 4   KQKEAMNFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKL---------RMRPQARIT 63
           K+    NF  +W I VS   Q F LAL+   L  L   L KL          +   ++I 
Sbjct: 46  KEDYFTNFADRWLIFVSMLLQ-FILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKIL 105

Query: 64  PMKSLIVRPEERYKEWKIIV------PDDKSMSEEEREFRYYSALTMMASKLAYQHHLFV 123
             K   V+ +E   E+  +V       D  S  + E+ FRYYSALT+MA+K++Y+   FV
Sbjct: 106 RGKG-FVKVKEDSSEYTSVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFV 165

Query: 124 NFVATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMV 183
             V    WKM  +  +NF N +    +T AFTF+NT+ D  V +VAFRGTS  D  DW V
Sbjct: 166 QSVVNDRWKMKLLGYFNFWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQV 225

Query: 184 DSNFLFKKLDGVGQIHSGFMDAIGYQ-----DQPIALHECHDKFAYHTLCQELKEIAKSN 243
           D +  + +++GVG+IHSGFM A+G Q      + +   + H+ FAY+TL ++L++IAK+N
Sbjct: 226 DVDLSWYEIEGVGRIHSGFMKALGLQKATGWPKELPKTQTHE-FAYYTLRKQLRDIAKAN 285

Query: 244 ENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTF 303
           E A+FI TGHSLGGALATLFVT+L+ + E+TIL+KL +VYTYGQPRVGD +FA FMV+  
Sbjct: 286 EKARFIFTGHSLGGALATLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCV 345

Query: 304 DNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIW 363
             +  KY+RYVYS+D++PRVP DA+L  YKHFG CVYF+  Y G+ +KEQPN NYFSL+W
Sbjct: 346 QKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLW 405

Query: 364 VIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRW 410
           VIPKYL A WELIRS  IIP  KG D++E L     R++GLLIPGLSAH   NYVN+TR 
Sbjct: 406 VIPKYLSAWWELIRS-FIIPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRL 465

BLAST of Clc05G22220 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.6e-79
Identity = 171/430 (39.77%), Postives = 248/430 (57.67%), Query Frame = 0

Query: 5   QKEAMNFKTQWAILVSCWTQKFFLALVVPV----------LKILGTILGKLRMRPQARIT 64
           + +   F+ +W I VS   QK  +    P+          L +L +  G L + P     
Sbjct: 43  EDDLSRFRRRWIIFVSIVIQKLIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNL--- 102

Query: 65  PMKSLIVRPEERYKEWKIIVPD-----DKSMSEEEREFRYYSALTMMASKLAYQHHLFVN 124
             K  I+ PE+    +  +  +     + +   E    RY + L++MASKL+Y++  FV+
Sbjct: 103 -FKGNIIWPEKASATFASLNGNLDRRVELNPKVERGSKRYKAMLSIMASKLSYENINFVS 162

Query: 125 FVATYCWKMDHVECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVD 184
            V    WKMD +  Y+  N Y K +ST     ++TS D  + +V+FRGT  FD +DW  D
Sbjct: 163 SVLHNHWKMDLLGFYSCWNGYQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTD 222

Query: 185 SNFLFKKLDGVGQIHSGFMDAIGYQDQ----PIALHECHDK---FAYHTLCQELKEIAKS 244
            +  + ++  VG+IH GFM A+G Q +     + L E  +    +AY+T+ + LKEI   
Sbjct: 223 LDLSWYEVKNVGKIHGGFMKALGLQKEGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQ 282

Query: 245 NENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDT 304
           N  +KFI+TGHSLGGALA LF  +L  + E  +L++L+ VYT+GQPRVGD+EF +FM D+
Sbjct: 283 NPTSKFILTGHSLGGALAILFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDS 342

Query: 305 FDNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLI 364
                +KY RYVY  DM+PR+PFD     +KHFG C+Y+D FY GK  +E+PN NYF+L+
Sbjct: 343 LKKFDVKYERYVYCNDMVPRLPFDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLV 402

Query: 365 WVIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTR 411
           WV+PK + A WELIRS  ++P  KG +  EG F    R++ LLIPGL AH  + Y+N+T 
Sbjct: 403 WVLPKIMNALWELIRS-FVMPYWKGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTL 462

BLAST of Clc05G22220 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.6e-74
Identity = 166/426 (38.97%), Postives = 241/426 (56.57%), Query Frame = 0

Query: 11  FKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRPQAR-------ITPMKSLIVRP 70
           F+ +W I VS   QK  + L  P L  LG  LG     P +        +  +K   + P
Sbjct: 49  FRGRWIIFVSIVVQKLLIILRKP-LSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWP 108

Query: 71  EERYKEWKII-------VPDDKSMSEEEREFRYYSALTMMASKLAYQHHLFVNFVATYCW 130
           E+    +  I       V      S +  + RY   L++MASKLAY++  F+  V    W
Sbjct: 109 EKTSATFASINGNLDQKVELGLGRSIKIGDERYKPLLSIMASKLAYENEHFIRSVLQDHW 168

Query: 131 KMDHVECYNFQNPYLKTKSTHAFTFQNT--SKDVTVVAFRGTSVFDLNDWMVDSNFLFKK 190
           +MD +  Y+  N + +T+ST     ++T  + ++ VV+FRGT  F+ +DW  D +  +  
Sbjct: 169 QMDLLGFYSCPNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHN 228

Query: 191 LDGVGQIHSGFMDAIGYQD----QPIALHECHDK---FAYHTLCQELKEIAKSNENAKFI 250
           +  VG+IH GFM A+G       + I   +  +K    AY+T+ ++LKE+ + N  +KFI
Sbjct: 229 VMNVGKIHGGFMKALGLPKEGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFI 288

Query: 251 ITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIK 310
           ++GHSLGGALA LF  +L  + E  +L++L+ VYT+GQPRVGD++F  +M D      +K
Sbjct: 289 LSGHSLGGALAILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVK 348

Query: 311 YYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYL 370
           Y RYVY  DM+PR+PFD     +KHFGGC+Y D FY GK  +E+PN NYF++ WVIPK +
Sbjct: 349 YKRYVYCNDMVPRLPFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKII 408

Query: 371 IACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTI--Q 412
            A WELIRS  II   +GR++ EG      R++ LLIPGL AH  + YVN+   G    Q
Sbjct: 409 NAVWELIRS-FIISCWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQ 468

BLAST of Clc05G22220 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 267.3 bits (682), Expect = 2.1e-71
Identity = 153/415 (36.87%), Postives = 238/415 (57.35%), Query Frame = 0

Query: 10  NFKTQWAILVSCWTQKFFLALVVPVLKILGTILGKLRMRP------QARITPMKSLIVRP 69
           +F ++W + ++ + QK  + L  P   I   +   L +           +  M   +V+P
Sbjct: 53  SFASRWILALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKP 112

Query: 70  EERYKEW-KIIVPDDKSMSEEER----EFRYYSALTMMASKLAYQHHLFVNFVATYCWKM 129
           ++    +   I   D+ +  +E+       Y S L++MASK++Y+   ++  V    WKM
Sbjct: 113 DKSSATYTSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSVVKNTWKM 172

Query: 130 DHVECYNFQNPYLKTKSTHAFTFQ--NTSKDVTVVAFRGTSVFDLNDWMVDSNFLFKKLD 189
           D V  Y+F N + ++K T AF F+  +T+ D+ VV+FRGT  F+  DW  D +  + ++ 
Sbjct: 173 DLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMK 232

Query: 190 GVGQIHSGFMDAIGYQDQ--PIALHECHDKFAYHTLCQELKEIAKSNENAKFIITGHSLG 249
            VG++H+GF  A+G Q    P        ++AY+T+ Q L++    N+N K+I+TGHSLG
Sbjct: 233 NVGKVHAGFSRALGLQKDGWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLG 292

Query: 250 GALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDTFDNHHIKYYRYVYS 309
           GALA LF  +LA + E  +LDKL+ +YT+GQPRVGD++F  FM      H I+Y R+VY+
Sbjct: 293 GALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYN 352

Query: 310 FDMIPRVPF-DAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLIWVIPKYLIACWEL 369
            D++PRVPF D  L +YKH+G C  F+  Y GK  ++ PN NYF+L+W+IP+ L   WE 
Sbjct: 353 NDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEF 412

Query: 370 IRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTRWGTIQLPP 409
           IRS  I+   KG ++ E       R++G++ PG S H   +YVN TR G +  PP
Sbjct: 413 IRS-FILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPP 466

BLAST of Clc05G22220 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-66
Identity = 148/420 (35.24%), Postives = 234/420 (55.71%), Query Frame = 0

Query: 5   QKEAMNFKTQWAILVSCWTQK---FFLALVVPVLKILGTILGKLRMRPQARITPMKSLIV 64
           + E  +F+ +W I VS    K   FF  L+  V   L   L  L     + +  ++  +V
Sbjct: 45  ETEEHSFRHRWLIFVSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNSFSGLF-LRGEVV 104

Query: 65  RPEERYKEWKIIVPD-------DKSMSEEEREFRYYSALTMMASKLAYQHHLFVNFVATY 124
            P+   + ++  +         D +++ E+ E +YY+AL++MASK+AY++   +  V   
Sbjct: 105 MPQRTSENYQSFIGHLDTRVSLDMTLNREDGE-KYYAALSIMASKIAYENAARIKHVVEN 164

Query: 125 CWKMDHVECYNFQNPYLKTKSTHAF--------TFQNTSKDVTVVAFRGTSVFDLNDWMV 184
            W M ++   ++ N Y + ++T AF        T  N  +   VVAFRGT +F+  DW  
Sbjct: 165 HWNMKYLGLVDYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCS 224

Query: 185 DSNFLFKKLDGVGQIHSGFMDAIGYQD------QPIALHECHDKFAYHTLCQELKEIAKS 244
           D +  + +L  +G IH GFM A+G Q+      +P++  +     AY+++   LK +   
Sbjct: 225 DFDITWFELPNIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQ 284

Query: 245 NENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFMVDT 304
           N+N KF++TGHSLGGALA LF  +L  + E  +L+++Q VYTYGQPRVGD +F  FM   
Sbjct: 285 NKNTKFVLTGHSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKK 344

Query: 305 FDNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYFSLI 364
            + ++IKYYR+VY+ D++PR+P+D     +KHFG C+Y+D  Y  K ++EQ + N+F L 
Sbjct: 345 LEKYNIKYYRFVYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLR 404

Query: 365 WVIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVNLTR 401
            +I     A  E IRS  I+   KG ++ EG      R LG+++PG+S H   +YVN TR
Sbjct: 405 GIIKMMWSAILEFIRSFTIV-AEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATR 461

BLAST of Clc05G22220 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 140/365 (38.36%), Postives = 204/365 (55.89%), Query Frame = 0

Query: 67  YKEWKI------IVPDDKSMSEEEREFRYYSALTMMASKLAYQHHLFVNFVATYCWKMDH 126
           YKEW        I   D    + E   R    L +MASKLAY++   V  V    WKM+ 
Sbjct: 145 YKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVVDLHWKMNL 204

Query: 127 VECYNFQNPYLKTKSTHAFTFQNTSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGV 186
           VE  +  N Y K  ST  F F +  KD  + V++FRGT  FD +DW  D ++ + ++  V
Sbjct: 205 VEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDYSWYEVPNV 264

Query: 187 GQIHSGFMDA--IGYQDQPIALH-------------------ECHDKFAYHTLCQELKEI 246
           G++H GF++A  +G +D     H                   +  ++ AY+ +   LK +
Sbjct: 265 GKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRL 324

Query: 247 AKSNENAKFIITGHSLGGALATLFVTLLAYYKEATILDKLQAVYTYGQPRVGDDEFAHFM 306
              +ENA+F++TGHSLGGALA LF TLL   +E  I+ +L  VYT+GQPR+G+ E   FM
Sbjct: 325 LSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFM 384

Query: 307 VDTFDNHHIKYYRYVYSFDMIPRVPFDAMLTNYKHFGGCVYFDCFYNGKFLKEQPNTNYF 366
               +    +Y+R VY  D++PR+P+D     YKHFG C+++D FYN    +++P+ N +
Sbjct: 385 KAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPY 444

Query: 367 SLIWVIPKYLIACWELIRSLIIIPITKGRDHFEGLFTILERILGLLIPGLSAHVCSNYVN 403
            L + I  ++IA WEL+R L  +  T G D+ EG F IL R++GL+IPGLS H  ++YVN
Sbjct: 445 GLRYKILGHVIAVWELVRGL-TMGYTHGPDYKEGWFRILFRLMGLVIPGLSDHCMTDYVN 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031739748.17.1e-13859.40uncharacterized protein LOC105435389 [Cucumis sativus][more]
KGN55200.17.1e-13859.40hypothetical protein Csa_012841 [Cucumis sativus][more]
XP_038896694.13.5e-13759.00triacylglycerol lipase OBL1-like [Benincasa hispida][more]
XP_022936593.13.3e-12756.22uncharacterized protein LOC111443149 [Cucurbita moschata][more]
KAG6591383.11.2e-12655.99Lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
F4JFU81.9e-6138.36Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
A0A1S3ZP855.4e-5633.96Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
Q5VKJ71.6e-5537.17Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
P195151.8e-1131.58Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
P618719.3e-0828.14Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KZS73.4e-13859.40Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE... [more]
A0A6J1FDN51.6e-12756.22uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC1114431... [more]
A0A6J1CEU23.7e-10049.76uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1CEI91.1e-9948.64uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A1S3BUY02.6e-9849.07uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=... [more]
Match NameE-valueIdentityDescription
AT5G42930.11.6e-7939.77alpha/beta-Hydrolases superfamily protein [more]
AT1G56630.12.6e-7438.97alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.12.1e-7136.87triacylglycerol lipase-like 1 [more]
AT5G67050.11.5e-6635.24alpha/beta-Hydrolases superfamily protein [more]
AT3G14360.11.4e-6238.36alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 78..339
e-value: 3.2E-47
score: 163.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 95..333
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 150..304
e-value: 7.2E-35
score: 119.9
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..406
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..406
NoneNo IPR availableCDDcd00519Lipase_3coord: 87..320
e-value: 1.08185E-40
score: 142.615

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc05G22220.1Clc05G22220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0004806 triglyceride lipase activity