Clc05G22100 (gene) Watermelon (cordophanus) v2

Overview
NameClc05G22100
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionAlpha-amylase
LocationClcChr05: 30464390 .. 30468495 (-)
RNA-Seq ExpressionClc05G22100
SyntenyClc05G22100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGCAAGCCTTCTTTCTCTCTGTTCCTTCAGCTCATTATCAAGAACATTTGCTTAAATTTGTATAAAACCTCTTACAGGATACTTACCTGGAAGGCTTTATGATCTCAATGCATCCAAATACGGAACCCAGAATGAACTCAAGTCCCTAATTGCCGCCCTTCATCAGAAAGGAATCAAGGCCATAGCTGACATAGTAATCAACCACAGAACAGCAGAGAAACAAGATCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGTGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGCGAACCCTATCTACCAGCCCCAGATATCGATCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAAGGATATGCACCAAGTATTACAAAGCTCTACATGGATCAAACCTTGCCAGATTTTGCAGTTGGTGAGAAATGGGATGAACTTTCAGAAGGACAGGATAGGAAACCCAATCAAAATCAAGACGCACATCGCGATGCTCTTGCAAATTGGGTTAGGGCTGGGGGAGATACGGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAAGGAGAGCTATGGAGGTTGAGAGATTCTAACGGGAAGCCGCCGGGATTGATCGGAATCAAACCGGAAAGTGCCGTAACTTTCATCGATAACCACGACACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATCCAGGGATACGCATATATTCTCACTCATCCTGGAACGCCTTCCATTGTAATTGATTCAACAAAATCAGTTTAGAAATTTGAGTTTCGAAACTTAATTACCTGTAATTGACATACTGTTGGTTTGTTGCAGTTTTACGACCATTTCGTCGATTGGGGATTGAAGGAGGAGATAATGAAATTGAATGCAATTAGAGCAAGGAATGGGATTGGGAAAACGAGCACGCTGAATATCATGGCATCTCAGGGGGATTTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATTGGAGCGAACACCAATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCTACTTCTGGAATGGAGTATGCTGTTTGGGAGAAAAAGTGAATTCTTGAATGTCTGCCATTGATGGATTAATTCCCAAGCTCCATCTCCATTTTCAAAAAGATCTGACATAATATATACTGCACCTATCTTTGTACTTGGTTGCATCTCATAAATAATAAAGCAGTCCTAATTATCTTTGAATATGGAAATAATCATCTAGATTAACTAATCTAATGCAAACTTAAATCCAAAAATAAAGAATATGTAGGTAGTTCGAGAGAGTCTAAGACTTTCTAATCTTTTAGTGTATGTTTGGGAGTGATTTTAAATTATTAGAATCAATTTTGTTATGTTCAAAATTACTCGGAATCACACTTTCAACTCTTAAAATTAATTTAATTTTTAATTTTCTACTTTAAAATATAATTTTTATATCATCAAAATTAATCAAAAACATATCTCAAGGTAAGGTGATTTTAGTAATGATAAAAGAGATTTTAACCCTTTCAATTACATAGCCTTATATTAAATCCAAGACTTGTAAATATCATATCAACGTCATTCATCATCATTAAAGACAACTCAAATTCAAGCCAAATTCTTCATATATACTTACAAAATTACTAGATAGATCCAAAGTAGACGAGATATGAAACAATACACTGACTGAGGTAAGAAAAGTTTCATTACCAAATCTCTTGTTTACGTATTTGTTTTGGATTGAAATTTGAATAGCGAGGCAATAGAGCAATAATAAGAGCATAGTTACTCCAATTCCAATCTACCTGTTGACTAGGGGCTGCTGCTAAGTTCCTAACAAACTCATAATTCTCATTTCAATCCATTCCCACTTACACTTTTGTTCAAGAGATCATCTTTTCGACATCAATTTTAAAGTTAATTTCGAGTCTTTATTCAATGTTAAGTTATTCCTCAGCAGTAATCGTTCAATCACTGACCCGACAACAGATCTGACCATCCAAAACTAAAGACCTTTCAAAATGTTGAATTGGTTTGTTTGATGGAAGTACAATGGCGGCAAAACTTGATTGGTTTCAGAGTCCATGTTTCTTTTTCATTTTGACTTTAGGCAGATTTCTCTTTTCTCCTTCTCGCTTAACTCAACATAAAGTTAAGCCAATACGTTGACAAATCCCGTATAAATAGGCATGGAAGCTCCTCATCCAGCAAAAAGCTATGGCTTCTCCATCTCCATGGTGCTTCTTTCTCCTGCTTGCTCTTGTCTTCCCCTCCTCTACTACTACCCAAATTTTGTTTCAGGTTCTGCAGTTTTCTCATTACCATCTTCAATTTGATCTGTATATATACAACTCCATGATTCCTTAATAAGAAACTTGTTTGTGTCCATATCGGAGCAGGGTTTCAACTGGGATTCAAAAAGCTCTGCTGGCTGGTATAACATTCTGAAGACCAAGATCCCCGATCTCGTTAATGCCGGAATCACCCATGTTTGGCTTCCTCCTCCTTCTAACTCCCATCCCAACGGTTCAGAAGGTTCTTCTAAAATCTAAATCATCTTCCTTTCAACCCCAGATTGCAGAAGCTACAGAATTTTCCTAAAACTGATTTACATAACGCTTGTTGTTCTCTACTTCTACAGGATACTTGCCTGGTAGACTTTATGATCTTGACACCTCCAAATATGGAAATAAGGATGAATTAAAGGCGTTAATTGCAGATCTTCGTCGCAACGGCATCAAAGCCATCGCTGACATTGTGATCAATCACAGAACTGCCGAAAGACAAGATTCAAATGGCAGATGGTCTATCTTCGAAGGGGGGACTCCAGATAATCGTCTTGATTGGAACATATCATTCGTTTGCAAAAATGATAGCCCATTCTCTGGAGATGGAAATCCAGACACTGGAATGGACTGGGGAGAAGCACCAGACATAGACCATTTGAACCCACAAGTTCAAACAGAGTTATCGGACTGGATGAACTGGCTCAAATCTGATGTTGGCTTCATTGGATGGCGATTTGATATGGTCGTCGGCTATACACCAATGGCAACCAAAATCTACATGGAGAGAACCTCGCCGGAGTTCGCCGTTGGCGAGAAATGGGACAATCCGCCTGGTAGGGTTGTCCAGGAGCAGGACCGTCGTGCACTTCTTGAATGGATTCAGGCAGCCGGAGGAGCCGTCGCCGCCTTCGATTTCACTACCAAGTTCGTCCTCCACGCCGCCGTCAAGGGCGAGCTATGGCGGCTGAAGGATTCAAGCAATCGGCCACCTGGTTTGATAGGGCTAAAACCAGAGAACGCCGTAACATTCATCGATAATCATGACACATTCTCTCAGAGATCGGCTCCATTCCCAGAGGGCAAAGTCATGCTAGGCTATGTTTACATTCTCACCCACCCCGGAACCCCAACAATCGTAATTTCTGAATCATATTCCAACGAGATTAGGTTTTCAGCCATTAATTTCTATTAATAACTTTTGAACCTTGATTCATTGACAGTTCTACGACCACCTATTGGAGTGGGGACAAACCCTACAGAAACCGATCGTGGAGCTTACAACAGTGAGGAAGAGGATTGGGATAAACAACAAAAGCAAAGTGAGGATAATCACGGCAGAGGCTGACCTATACATGGCGGAAATTGAGGAGAAGATGATTGTGAAGATTGGAGGAAGGTTGAACCTGGGGAATCTTCTTCCTCCAACCTACAGAGAGATCGCCACCATATCTGGAGAAGACTATGCTGTTTGGGAGAAGAAGTGAAGGTTGTAAGTTATATAAAGAAACAATATTGTAATAAATAATAATGTGGAAGAGTAACACCGCTTCCATATATGATCTACCCAGTCTCTGTTCTGTTGGTGTAATTAATGTCCGCCATTCTATGATCGTATAGCAATCAATATATATATGGAAA

mRNA sequence

ATGTCTGGCTTCCTCCACCCTCCCAATCAGCTTTATGATCTCAATGCATCCAAATACGGAACCCAGAATGAACTCAAGTCCCTAATTGCCGCCCTTCATCAGAAAGGAATCAAGGCCATAGCTGACATAGTAATCAACCACAGAACAGCAGAGAAACAAGATCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGTGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGCGAACCCTATCTACCAGCCCCAGATATCGATCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAAGGATATGCACCAAGTATTACAAAGCTCTACATGGATCAAACCTTGCCAGATTTTGCAGTTGGTGAGAAATGGGATGAACTTTCAGAAGGACAGGATAGGAAACCCAATCAAAATCAAGACGCACATCGCGATGCTCTTGCAAATTGGGTTAGGGCTGGGGGAGATACGGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAAGGAGAGCTATGGAGGTTGAGAGATTCTAACGGGAAGCCGCCGGGATTGATCGGAATCAAACCGGAAAGTGCCGTAACTTTCATCGATAACCACGACACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATCCAGGGATACGCATATATTCTCACTCATCCTGGAACGCCTTCCATTTTTTACGACCATTTCGTCGATTGGGGATTGAAGGAGGAGATAATGAAATTGAATGCAATTAGAGCAAGGAATGGGATTGGGAAAACGAGCACGCTGAATATCATGGCATCTCAGGGGGATTTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATTGGAGCGAACACCAATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCTACTTCTGGAATGGAGTATGCTGCATGGAAGCTCCTCATCCAGCAAAAAGCTATGGCTTCTCCATCTCCATGGTGCTTCTTTCTCCTGCTTGCTCTTGTCTTCCCCTCCTCTACTACTACCCAAATTTTGTTTCAGGGTTTCAACTGGGATTCAAAAAGCTCTGCTGGCTGGTATAACATTCTGAAGACCAAGATCCCCGATCTCGTTAATGCCGGAATCACCCATGTTTGGCTTCCTCCTCCTTCTAACTCCCATCCCAACGGTTCAGAAGGATACTTGCCTGGTAGACTTTATGATCTTGACACCTCCAAATATGGAAATAAGGATGAATTAAAGGCGTTAATTGCAGATCTTCGTCGCAACGGCATCAAAGCCATCGCTGACATTGTGATCAATCACAGAACTGCCGAAAGACAAGATTCAAATGGCAGATGGTCTATCTTCGAAGGGGGGACTCCAGATAATCGTCTTGATTGGAACATATCATTCGTTTGCAAAAATGATAGCCCATTCTCTGGAGATGGAAATCCAGACACTGGAATGGACTGGGGAGAAGCACCAGACATAGACCATTTGAACCCACAAGTTCAAACAGAGTTATCGGACTGGATGAACTGGCTCAAATCTGATGTTGGCTTCATTGGATGGCGATTTGATATGGTCGTCGGCTATACACCAATGGCAACCAAAATCTACATGGAGAGAACCTCGCCGGAGTTCGCCGTTGGCGAGAAATGGGACAATCCGCCTGGTAGGGTTGTCCAGGAGCAGGACCGTCGTGCACTTCTTGAATGGATTCAGGCAGCCGGAGGAGCCGTCGCCGCCTTCGATTTCACTACCAAGTTCGTCCTCCACGCCGCCGTCAAGGGCGAGCTATGGCGGCTGAAGGATTCAAGCAATCGGCCACCTGGTTTGATAGGGCTAAAACCAGAGAACGCCGTAACATTCATCGATAATCATGACACATTCTCTCAGAGATCGGCTCCATTCCCAGAGGGCAAAGTCATGCTAGGCTATGTTTACATTCTCACCCACCCCGGAACCCCAACAATCTTCTACGACCACCTATTGGAGTGGGGACAAACCCTACAGAAACCGATCGTGGAGCTTACAACAGTGAGGAAGAGGATTGGGATAAACAACAAAAGCAAAGTGAGGATAATCACGGCAGAGGCTGACCTATACATGGCGGAAATTGAGGAGAAGATGATTGTGAAGATTGGAGGAAGGTTGAACCTGGGGAATCTTCTTCCTCCAACCTACAGAGAGATCGCCACCATATCTGGAGAAGACTATGCTGTTTGGGAGAAGAAGTGAAGGTTGTAAGTTATATAAAGAAACAATATTGTAATAAATAATAATGTGGAAGAGTAACACCGCTTCCATATATGATCTACCCAGTCTCTGTTCTGTTGGTGTAATTAATGTCCGCCATTCTATGATCGTATAGCAATCAATATATATATGGAAA

Coding sequence (CDS)

ATGTCTGGCTTCCTCCACCCTCCCAATCAGCTTTATGATCTCAATGCATCCAAATACGGAACCCAGAATGAACTCAAGTCCCTAATTGCCGCCCTTCATCAGAAAGGAATCAAGGCCATAGCTGACATAGTAATCAACCACAGAACAGCAGAGAAACAAGATCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGTGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGCGAACCCTATCTACCAGCCCCAGATATCGATCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAAGGATATGCACCAAGTATTACAAAGCTCTACATGGATCAAACCTTGCCAGATTTTGCAGTTGGTGAGAAATGGGATGAACTTTCAGAAGGACAGGATAGGAAACCCAATCAAAATCAAGACGCACATCGCGATGCTCTTGCAAATTGGGTTAGGGCTGGGGGAGATACGGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAAGGAGAGCTATGGAGGTTGAGAGATTCTAACGGGAAGCCGCCGGGATTGATCGGAATCAAACCGGAAAGTGCCGTAACTTTCATCGATAACCACGACACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATCCAGGGATACGCATATATTCTCACTCATCCTGGAACGCCTTCCATTTTTTACGACCATTTCGTCGATTGGGGATTGAAGGAGGAGATAATGAAATTGAATGCAATTAGAGCAAGGAATGGGATTGGGAAAACGAGCACGCTGAATATCATGGCATCTCAGGGGGATTTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATTGGAGCGAACACCAATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCTACTTCTGGAATGGAGTATGCTGCATGGAAGCTCCTCATCCAGCAAAAAGCTATGGCTTCTCCATCTCCATGGTGCTTCTTTCTCCTGCTTGCTCTTGTCTTCCCCTCCTCTACTACTACCCAAATTTTGTTTCAGGGTTTCAACTGGGATTCAAAAAGCTCTGCTGGCTGGTATAACATTCTGAAGACCAAGATCCCCGATCTCGTTAATGCCGGAATCACCCATGTTTGGCTTCCTCCTCCTTCTAACTCCCATCCCAACGGTTCAGAAGGATACTTGCCTGGTAGACTTTATGATCTTGACACCTCCAAATATGGAAATAAGGATGAATTAAAGGCGTTAATTGCAGATCTTCGTCGCAACGGCATCAAAGCCATCGCTGACATTGTGATCAATCACAGAACTGCCGAAAGACAAGATTCAAATGGCAGATGGTCTATCTTCGAAGGGGGGACTCCAGATAATCGTCTTGATTGGAACATATCATTCGTTTGCAAAAATGATAGCCCATTCTCTGGAGATGGAAATCCAGACACTGGAATGGACTGGGGAGAAGCACCAGACATAGACCATTTGAACCCACAAGTTCAAACAGAGTTATCGGACTGGATGAACTGGCTCAAATCTGATGTTGGCTTCATTGGATGGCGATTTGATATGGTCGTCGGCTATACACCAATGGCAACCAAAATCTACATGGAGAGAACCTCGCCGGAGTTCGCCGTTGGCGAGAAATGGGACAATCCGCCTGGTAGGGTTGTCCAGGAGCAGGACCGTCGTGCACTTCTTGAATGGATTCAGGCAGCCGGAGGAGCCGTCGCCGCCTTCGATTTCACTACCAAGTTCGTCCTCCACGCCGCCGTCAAGGGCGAGCTATGGCGGCTGAAGGATTCAAGCAATCGGCCACCTGGTTTGATAGGGCTAAAACCAGAGAACGCCGTAACATTCATCGATAATCATGACACATTCTCTCAGAGATCGGCTCCATTCCCAGAGGGCAAAGTCATGCTAGGCTATGTTTACATTCTCACCCACCCCGGAACCCCAACAATCTTCTACGACCACCTATTGGAGTGGGGACAAACCCTACAGAAACCGATCGTGGAGCTTACAACAGTGAGGAAGAGGATTGGGATAAACAACAAAAGCAAAGTGAGGATAATCACGGCAGAGGCTGACCTATACATGGCGGAAATTGAGGAGAAGATGATTGTGAAGATTGGAGGAAGGTTGAACCTGGGGAATCTTCTTCCTCCAACCTACAGAGAGATCGCCACCATATCTGGAGAAGACTATGCTGTTTGGGAGAAGAAGTGA

Protein sequence

MSGFLHPPNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWVRAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMASPSPWCFFLLLALVFPSSTTTQILFQGFNWDSKSSAGWYNILKTKIPDLVNAGITHVWLPPPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQDSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFSGDGNPDTGMDWGEAPDIDHLNPQVQTELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDNPPGRVVQEQDRRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPENAVTFIDNHDTFSQRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTLQKPIVELTTVRKRIGINNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLLPPTYREIATISGEDYAVWEKK
Homology
BLAST of Clc05G22100 vs. NCBI nr
Match: KAF1886612.1 (hypothetical protein Lal_00045845 [Lupinus albus])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.8e-309
Identity = 519/797 (65.12%), Postives = 607/797 (76.16%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYG++ ELKSLIAALH KGIKA+ADIVINHRTAE++D+RGI+ IFEGGT 
Sbjct: 75  PGRLYDLDASKYGSKEELKSLIAALHSKGIKAVADIVINHRTAERKDERGIYSIFEGGTS 134

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D +LDWGP FIC DDT YSDG GN DSGE Y  APDIDHLNPQ QKE+SEWM WLKTE+G
Sbjct: 135 DGKLDWGPHFICKDDTAYSDGTGNLDSGEGYAAAPDIDHLNPQAQKELSEWMNWLKTEVG 194

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWRFDFVKGYAPSITK+YM++T PDFAVGE W+ LS  QD KP++NQD HR AL NWV
Sbjct: 195 FDGWRFDFVKGYAPSITKIYMEKTSPDFAVGENWNSLSY-QDGKPDKNQDGHRGALVNWV 254

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
              G  V AFDFTTKGILQAAV+GELWRL+DSNGKP GLIG+KPE+AVTFIDNHDTGSTQ
Sbjct: 255 EGAGGVVNAFDFTTKGILQAAVEGELWRLKDSNGKPSGLIGVKPENAVTFIDNHDTGSTQ 314

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSI-------------------FYDHFVDWGLKEEIM 307
             WPFPSDKV+QGYAYILTHPGTPSI                   FYDHF +WGLKEEI 
Sbjct: 315 NSWPFPSDKVMQGYAYILTHPGTPSIFIGARILSGIRVYNTLECEFYDHFFNWGLKEEIA 374

Query: 308 KLNAIRARNGIGKTSTLNIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGM 367
           KL AIR +NGI   S+++I+A+  DLYVA +D KII+KIG   ++GNLIPS++ +A SG 
Sbjct: 375 KLTAIRLKNGINSKSSVDILAADADLYVAKIDNKIIVKIGPKMDVGNLIPSNFHVAASGK 434

Query: 368 EYAAWKLLIQQKAMASPSPWCFFLLLALVFPSSTTTQILFQGFNW-DSKSSAGWYNILKT 427
            YA W                             T   +  GFNW  S    GWYN LKT
Sbjct: 435 NYAVW--------------------------LEYTKMNMCMGFNWASSDKEGGWYNFLKT 494

Query: 428 KIPDLVNAGITHVWLPPPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGI 487
            +PD+ +AG+ +VWLPP SNSH +G +GYLP RLYDLDTSKYGNK+E K+LI      G+
Sbjct: 495 LVPDIADAGVNYVWLPPLSNSHDDGPQGYLPKRLYDLDTSKYGNKEESKSLIDAFSERGV 554

Query: 488 KAIADIVINHRTAERQDSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFSGDGNPDTGMDW 547
           K+++DIVINHRTAER D+NG  SIFEGGTPDNRLDWN+S++C ND+ F+G GN DTG DW
Sbjct: 555 KSMSDIVINHRTAERLDNNG-LSIFEGGTPDNRLDWNVSYICGNDTRFNGTGNLDTGDDW 614

Query: 548 GEAPDIDHLNPQVQTELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVG 607
             APDIDH+NP VQ ELSDWMNWLK++VGF+GWRFDMVVGY P  TKIYME+TSP+FAVG
Sbjct: 615 WGAPDIDHVNPNVQKELSDWMNWLKTEVGFVGWRFDMVVGYAPRFTKIYMEQTSPDFAVG 674

Query: 608 EKWDN----PPGRVVQEQD--RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKD 667
           E + N      GR +  QD  R  L+ W+  AGG V AFDFTTK VL AAV+GELWRLKD
Sbjct: 675 ELYRNVTRGSDGRPLANQDEHRSTLVNWVNDAGGVVTAFDFTTKMVLGAAVQGELWRLKD 734

Query: 668 SSNRPPGLIGLKPENAVTFIDNHDTFSQRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLL 727
            + +PPG+IG KPENAVTF+DNHDT SQ++ PFP  KVMLGYVYILTHPG PTIFYDH +
Sbjct: 735 DNGKPPGMIGTKPENAVTFVDNHDTLSQQTWPFPNDKVMLGYVYILTHPGHPTIFYDHYI 794

Query: 728 EWGQTLQKPIVELTTVRKRIGINNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLL 779
           EWG  L++PI  +T +RKR GIN KS V I+ AEADLYMAEI+ K+IVKIG + +LGNLL
Sbjct: 795 EWG--LKEPIKNVTEIRKRNGINPKSTVNILAAEADLYMAEIDNKIIVKIGPKEDLGNLL 839

BLAST of Clc05G22100 vs. NCBI nr
Match: OMO78941.1 (hypothetical protein CCACVL1_14000 [Corchorus capsularis])

HSP 1 Score: 1037.7 bits (2682), Expect = 5.0e-299
Identity = 498/759 (65.61%), Postives = 586/759 (77.21%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYG+Q ELKSLI A HQ G+K +ADIVINHRTAE +D RGI+ IFEGGT 
Sbjct: 187 PGRLYDLDASKYGSQAELKSLIDAFHQNGVKCVADIVINHRTAESKDSRGIYSIFEGGTS 246

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDWGPSFICSDD EYSDG GNPD+G PY PAPDIDHLNP+VQKE+S+WM WLKTEIG
Sbjct: 247 DDRLDWGPSFICSDDKEYSDGTGNPDTGLPYNPAPDIDHLNPRVQKELSDWMNWLKTEIG 306

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWRFDFV+GYAPSITK+YMDQT PDFAVGEKW++ S GQ       +D+HR AL +WV
Sbjct: 307 FDGWRFDFVRGYAPSITKIYMDQTSPDFAVGEKWEDFSLGQ-------EDSHRSALKDWV 366

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
            A G  VTAFDFTTKG+L AAVQG L RL+DSNGKPPGLIG+ P++AVTFIDNHDTGSTQ
Sbjct: 367 EAAGGAVTAFDFTTKGVLNAAVQGALGRLKDSNGKPPGLIGLLPQNAVTFIDNHDTGSTQ 426

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
            +WPFPSDKV+QGYAYILTHPG PSIFYDHF DW LK+EI KL AIR  NGI  TST+NI
Sbjct: 427 NLWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWDLKDEISKLVAIRNSNGINPTSTVNI 486

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMASPSP 367
           +AS  DLYVA +D KIIMKIG  +++GNL+PS+YQLA SG +Y+                
Sbjct: 487 LASDADLYVASIDDKIIMKIGPRSDVGNLVPSNYQLAASGKDYS---------------- 546

Query: 368 WCFFLLLALVFPSSTTTQILFQGFNWDS-KSSAGWYNILKTKIPDLVNAGITHVWLPPPS 427
                                 GFNW+S     GWYN LK  +PD+ NAG+THVW PPPS
Sbjct: 547 ----------------------GFNWESCNKGGGWYNSLKNFVPDIANAGVTHVWFPPPS 606

Query: 428 NSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQDSN 487
            S   G +GYLPGRLYDLD SKYG+K ELK+LI    + GIK +ADIVINHRTAER+D  
Sbjct: 607 QS--VGPQGYLPGRLYDLDASKYGSKAELKSLIEAFHQKGIKCLADIVINHRTAERKDGR 666

Query: 488 GRWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDIDHLNPQVQTELS 547
           G +SIFEGGTPD+RLDW  SF+C ND  +S G GNPDTG+D+  APDIDHLNP+VQ+ELS
Sbjct: 667 GIYSIFEGGTPDDRLDWGPSFICGNDREYSDGSGNPDTGLDYQPAPDIDHLNPRVQSELS 726

Query: 548 DWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKW-DNPPGRVVQEQDRR 607
           DWMNWLK+++GF GWRFD V GY P  TKIYMERTSP+FAVGEKW D   G+  Q+  R 
Sbjct: 727 DWMNWLKTEIGFDGWRFDFVRGYAPSITKIYMERTSPDFAVGEKWEDLSLGQGGQDWHRG 786

Query: 608 ALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPENAVTFIDNH 667
           +L +W++AAGG V AFDFTTK VL+AAV+GELWRLKDS+ +PPG+IGL P+NAVTFIDNH
Sbjct: 787 SLKDWVEAAGGVVKAFDFTTKGVLNAAVQGELWRLKDSNGKPPGMIGLLPQNAVTFIDNH 846

Query: 668 DTFS-QRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTLQKPIVELTTVRKRIGI 727
           DT S QR  PFP  KVM GY YILTHPGTP+IFYDH ++WG  L+  I +L  +R + GI
Sbjct: 847 DTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFIDWG--LKDEITKLAVIRNKNGI 896

Query: 728 NNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLLP 763
           +  S  RI+ +++DLYMA I+EK+I+KIG +++LGNLLP
Sbjct: 907 SATSTARILASDSDLYMAAIDEKIIMKIGPKMDLGNLLP 896

BLAST of Clc05G22100 vs. NCBI nr
Match: RXH99831.1 (hypothetical protein DVH24_021633 [Malus domestica])

HSP 1 Score: 1015.8 bits (2625), Expect = 2.0e-292
Identity = 496/855 (58.01%), Postives = 604/855 (70.64%), Query Frame = 0

Query: 8    PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
            P +LYDLNAS+YG Q+ELK+LI   H  GI++IADIVINHR AEK+D+RGIWCIFEGGTP
Sbjct: 385  PGRLYDLNASRYGNQDELKALINTFHDNGIQSIADIVINHRCAEKKDERGIWCIFEGGTP 444

Query: 68   DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
            D RLDWGPS ICSDDT YS+GKGNPD+G  +  APDIDH+N +VQ+E+S+WM WLKTEIG
Sbjct: 445  DDRLDWGPSLICSDDTAYSNGKGNPDTGADFSAAPDIDHINTRVQRELSDWMNWLKTEIG 504

Query: 128  FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
            F+GWRFDFVKGYAP  TKL++  T P F+VGE W+ L+ G D K   NQDAHR AL  WV
Sbjct: 505  FNGWRFDFVKGYAPEFTKLFVTNTRPSFSVGELWNSLAYGSDGKLEYNQDAHRRALVGWV 564

Query: 188  RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
               G  VTAFDFTTKGILQAAVQGELWR++DSNG  PG+IG+ P  +VTF+DNHDTGSTQ
Sbjct: 565  EGAGGDVTAFDFTTKGILQAAVQGELWRMKDSNGGAPGMIGLSPGKSVTFVDNHDTGSTQ 624

Query: 248  RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
             MWPFPSDKV+QGYAYILTHPG PSIFYDH+ DWGLKEEI KL AIR RNG+G  S L I
Sbjct: 625  NMWPFPSDKVMQGYAYILTHPGIPSIFYDHYFDWGLKEEITKLVAIRLRNGVGPDSALRI 684

Query: 308  MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK------------- 367
            +AS  DLYVA  D KII KIG   ++GNL+P  YQ+ATSG +Y  W+             
Sbjct: 685  LASDADLYVAATDEKIIAKIGPRYDVGNLVPPTYQIATSGNDYCVWEKKAGALFSGSNNL 744

Query: 368  ---------------LLIQQKAMASP---------------------------------- 427
                           LL +  +M +P                                  
Sbjct: 745  AEVRTVLMLPDLIQDLLQRFLSMENPVKPSLLAISKSFPLTRRPITARHVSTSKANHSAT 804

Query: 428  ----------SPWCFFLLLALVFPSSTTTQILFQGFNWDSKSSAGWYNILKTKIPDLVNA 487
                       P   FLL  L+ P+ T +QI+FQGFNW+S    GWYNILK  + DL ++
Sbjct: 805  RLKGDMNTRNVPLICFLLSILLTPNFTASQIIFQGFNWESSRKGGWYNILKQSVQDLASS 864

Query: 488  GITHVWLPPPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVI 547
            GITHVWLPP  +SH    EGY+PGRLYDL+ SK+GNKDELK+L++  R +GI+++ADIVI
Sbjct: 865  GITHVWLPP--SSHAASDEGYMPGRLYDLN-SKFGNKDELKSLVSAYRNSGIQSVADIVI 924

Query: 548  NHRTAERQDSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDID 607
            NHRTAE+QD  G W IFEGGT D+RLDW  S +C +D+ +S G GNPDTG D+G APDID
Sbjct: 925  NHRTAEKQDERGIWCIFEGGTDDDRLDWGPSLICGDDTKYSDGKGNPDTGEDFGGAPDID 984

Query: 608  HLNPQVQTELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDN-- 667
            H N +VQ ELSDWMNWLK+++GF GWRFD V GY P  TK+YM  TSP FAVGE W++  
Sbjct: 985  HKNTRVQKELSDWMNWLKTEIGFSGWRFDFVKGYAPEYTKLYMTNTSPNFAVGELWNSLS 1044

Query: 668  --PPGRVVQEQD--RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPG 727
                G+   +QD  R+AL+ W+++AGG V AFDFTTK +L AAV+GELWRLKDS+   PG
Sbjct: 1045 YGSDGKPNPDQDAHRQALVGWVESAGGGVTAFDFTTKGILQAAVQGELWRLKDSNGGAPG 1104

Query: 728  LIGLKPENAVTFIDNHDTFS-QRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTL 783
            +IGLKP ++VTFIDNHDT S Q   PFP  KVM GY YILTHPG P+IFYDH  +WG  L
Sbjct: 1105 MIGLKPGSSVTFIDNHDTGSTQHLWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWG--L 1164

BLAST of Clc05G22100 vs. NCBI nr
Match: KAB2635840.1 (alpha-amylase-like [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 952.6 bits (2461), Expect = 2.1e-273
Identity = 463/783 (59.13%), Postives = 555/783 (70.88%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDLNAS+YG Q+ELK+LI   H  GI++IADIVINHR AEK+D+RGIWCIFEGGTP
Sbjct: 78  PGRLYDLNASRYGNQDELKALINTFHDNGIQSIADIVINHRCAEKKDERGIWCIFEGGTP 137

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDWGPS ICSDD  YS+GKGNPD+G  +  APDIDH+N +VQ+E+S+WM WLKTEIG
Sbjct: 138 DDRLDWGPSLICSDDIAYSNGKGNPDTGADFSAAPDIDHINTRVQRELSDWMNWLKTEIG 197

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           F GWRFDFVKGYAP  TKL++ +T P F+VGE W+ L+ G D K   NQDAHR AL  WV
Sbjct: 198 FSGWRFDFVKGYAPEFTKLFVTKTRPSFSVGELWNSLAYGSDGKLEYNQDAHRRALVGWV 257

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
              G  VTAFDFTTKGILQAAVQGELWR++DSNG  PG+IG+ P  +VTFIDNHDTGSTQ
Sbjct: 258 EGAGGDVTAFDFTTKGILQAAVQGELWRMKDSNGGAPGMIGLSPGKSVTFIDNHDTGSTQ 317

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
            MWPFPSDKV+QGYAYILTHPG PSIFYDHF DWGLKEEI KL AIR RNGIG  S L I
Sbjct: 318 NMWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWGLKEEITKLVAIRLRNGIGPDSALRI 377

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMASPSP 367
           +AS  DLYVA +D KII KIG                                       
Sbjct: 378 LASDADLYVAAIDEKIIAKIGPR------------------------------------- 437

Query: 368 WCFFLLLALVFPSSTTTQILFQGFNWDSKSSAGWYNILKTKIPDLVNAGITHVWLPPPSN 427
                                           GWYNILK  + DL ++GITHVWLPP  +
Sbjct: 438 ------------------------------KGGWYNILKQSVQDLASSGITHVWLPP--S 497

Query: 428 SHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQDSNG 487
           S     EGY+PGRLYDL+T KYGNKDELK+L++  R +GI+++ADIVINHRTAE+QD  G
Sbjct: 498 SQAASDEGYMPGRLYDLNT-KYGNKDELKSLVSAYRDSGIQSVADIVINHRTAEKQDERG 557

Query: 488 RWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDIDHLNPQVQTELSD 547
            W IFEGGT D+RLDW  S +C +D+ +S G GNPDTG D+G APDIDH N +VQ ELSD
Sbjct: 558 IWCIFEGGTDDDRLDWGPSLICSDDTKYSDGKGNPDTGEDFGGAPDIDHKNTRVQKELSD 617

Query: 548 WMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDN----PPGRVVQEQD 607
           WMNWLK+++GF GWRFD V GY P  TK+YM  TSP FAVGE W++      G+   +QD
Sbjct: 618 WMNWLKTEIGFSGWRFDFVKGYAPEYTKLYMTNTSPNFAVGELWNSLSYGSDGKPNPDQD 677

Query: 608 --RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPENAVTF 667
             R+AL+ W+++AGG V AFDFTTK +L AAV+GELWRLKDS     G+IG+KP N+VTF
Sbjct: 678 AHRQALVRWVESAGGRVTAFDFTTKGILQAAVQGELWRLKDSKGGASGMIGVKPGNSVTF 737

Query: 668 IDNHDTFS-QRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTLQKPIVELTTVRK 727
           IDNHDT S Q   PFP  KVM GY YILTHPG P+IFYDH  +WG  L++ I ++T +R 
Sbjct: 738 IDNHDTGSTQHMWPFPSDKVMQGYAYILTHPGIPSIFYDHYFDWG--LKEEISKITAIRS 786

Query: 728 RIGINNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLLPPTYREIATISGEDYAVW 783
           R GI   S +RI+ A+AD+Y+A I+EK+IVKIG +++LG L+P  ++   + SG+DYAVW
Sbjct: 798 RNGIKPNSALRILAADADVYVASIDEKIIVKIGPKMDLGQLIPTNFQ--VSTSGKDYAVW 786

BLAST of Clc05G22100 vs. NCBI nr
Match: KAF2309818.1 (hypothetical protein GH714_005263 [Hevea brasiliensis])

HSP 1 Score: 945.7 bits (2443), Expect = 2.6e-271
Identity = 479/813 (58.92%), Postives = 577/813 (70.97%), Query Frame = 0

Query: 6   HPPNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGG 65
           + P +LYDL  SKYG+Q+ELKSLIAA HQ GIK +ADIVINHR+AEKQD+RG W IFEGG
Sbjct: 214 YKPGRLYDL-TSKYGSQDELKSLIAAFHQNGIKCVADIVINHRSAEKQDERGAWSIFEGG 273

Query: 66  TPDSRLDWGPSFICS-DDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKT 125
           T D+RLDW  SFICS DDT YS+GKGNPD+G  +    DIDHLNP+VQKE+S+WM WLK+
Sbjct: 274 TSDNRLDWDASFICSNDDTGYSNGKGNPDTGMDFPNVTDIDHLNPRVQKELSDWMNWLKS 333

Query: 126 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALA 185
           EIGFDGWR DFVKG++ +I K+YM+QT P+FAVGE WD  S GQD KP  + +AHR AL 
Sbjct: 334 EIGFDGWRLDFVKGFSTNIAKIYMEQTKPNFAVGEYWDFFSYGQDGKPVTDLNAHRRALR 393

Query: 186 NWVRAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTG 245
           +WV+A G+ VTAFDFTTKG LQAAVQGELWRL D++GKPPG IGI PE+AVTFIDNHDT 
Sbjct: 394 DWVQAVGNGVTAFDFTTKGALQAAVQGELWRLNDTSGKPPGFIGISPENAVTFIDNHDTW 453

Query: 246 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTST 305
           S Q++WPFPSDKV+ GY YILTHPGTPSI           E I K+ +IR   GI +TST
Sbjct: 454 S-QQVWPFPSDKVMLGYVYILTHPGTPSI-----------EPISKVASIRKIYGISETST 513

Query: 306 LNIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMAS 365
           + I+A++ DLY+A ++  +IMKIG   +LGNL+PS+++LAT G +YA W           
Sbjct: 514 VKILAAESDLYMAMINDNVIMKIGPKLDLGNLLPSNFELATPGQDYAVW----------- 573

Query: 366 PSPWCFFLLLALVFPSSTTTQILFQGFNWDS-KSSAGWYNILKTKIPDLVNAGITHVWLP 425
                                    GFNW+S     GWYN LK  IPDLVNAGITHVWLP
Sbjct: 574 -------------------------GFNWESCNKEGGWYNSLKNSIPDLVNAGITHVWLP 633

Query: 426 PPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQ 485
           PPS S     EGY+PGRLYDL  S YG +DELK+LI    + GIK +ADIVINHRTAE+Q
Sbjct: 634 PPSQS--VSCEGYMPGRLYDLKASTYGTQDELKSLIQAFHQKGIKCLADIVINHRTAEKQ 693

Query: 486 DSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDIDHLNPQVQT 545
           D+ G W IFEGGT D+RLDW  SF+C +D+ +S G GNPDTG D+  APDIDHLN +VQ 
Sbjct: 694 DNRGIWCIFEGGTSDDRLDWGPSFICSDDTAYSDGKGNPDTGDDFAPAPDIDHLNLRVQK 753

Query: 546 ELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDN----PPGRVV 605
           ELSDWMNWL S++GF GWRFD V GY P  TKIYME+TSP FAV EKWD+      G+  
Sbjct: 754 ELSDWMNWLNSEIGFDGWRFDFVKGYAPSITKIYMEKTSPNFAVSEKWDSLAYGQDGKPD 813

Query: 606 QEQD--RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPEN 665
             QD  R AL +WIQAAGG   AFDFTTK +L AAV+GELWRLKDS+ +PPGLIG+ P+N
Sbjct: 814 ANQDAHRGALKDWIQAAGGITTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGILPQN 873

Query: 666 AVTFIDNHDTFS-QRSAPFPEGKVMLGYVYILTHPGTPTI-------------------- 725
           AVTFIDNHDT S QR  PFP  KVM GY YILTHPG P+I                    
Sbjct: 874 AVTFIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGIPSIVTSAQSFQFYFSCDSNCTKR 933

Query: 726 ------FYDHLLEWGQTLQKPIVELTTVRKRIGINNKSKVRIITAEADLYMAEIEEKMIV 783
                 FYDH  +WG  L++ I +L ++RK  GI+++S V+I+ A++DLYMA I + +I+
Sbjct: 934 CFLPLQFYDHFFDWG--LKEQISKLASIRKTYGIHDRSTVKILAAQSDLYMATINDNIIM 971

BLAST of Clc05G22100 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 1.6e-167
Identity = 269/347 (77.52%), Postives = 306/347 (88.18%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYG++NELKSLIAA H+KGIK +ADIVINHRTAE++D RGI+CIFEGGTP
Sbjct: 75  PGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTP 134

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           DSR DWGPSFIC DDT YSDG GN DSGE Y  APDIDHLNPQVQ+E+SEWM WLKTEIG
Sbjct: 135 DSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIG 194

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWRFDFVKGYAPSI+K+YM+QT PDFAVGEKWD +S GQD KPN NQD+HR AL NWV
Sbjct: 195 FDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWV 254

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
            + G  +TAFDFTTKGILQAAVQGELWRL D NGKPPG+IG+KPE+AVTFIDNHDTGSTQ
Sbjct: 255 ESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQ 314

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
           R+WPFPSDKV+QGYAYILTHPGTPSIFYDHF DWGLKE+I KL++IR RNGI + ST+ I
Sbjct: 315 RLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKI 374

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
           MAS+GDLYVA +D KI++KIG   +LGNLIPS+  +ATSG +YA W+
Sbjct: 375 MASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

BLAST of Clc05G22100 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 489.6 bits (1259), Expect = 6.7e-137
Identity = 223/349 (63.90%), Postives = 271/349 (77.65%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYG + +LKSLI ALH KG+KAIADIVINHRTAE +D RGI+CIFEGGTP
Sbjct: 77  PGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTP 136

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D+RLDWGP  IC DD  Y+DG GNPD+G  +  APDIDHLN +VQKE+ EW+ WLK +IG
Sbjct: 137 DARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLNWLKADIG 196

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWRFDF KGY+  + K+Y+D++ P FAV E W  L+ G D KPN NQD HR  L NWV
Sbjct: 197 FDGWRFDFAKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWV 256

Query: 188 -RAGG-DTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGS 247
            + GG    T FDFTTKGIL  AV+GELWRLR ++GK PG+IG  P  AVTF+DNHDTGS
Sbjct: 257 DKVGGKGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGS 316

Query: 248 TQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTL 307
           TQ MWPFPSD+V+QGYAYILTHPGTP IFYDHF DWGLKEEI +L ++R R+GI   S L
Sbjct: 317 TQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHGIHNESKL 376

Query: 308 NIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
            I+ +  DLY+A +DGK+I+K+G   ++GNLIP  +++A  G +YA W+
Sbjct: 377 QIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWE 425

BLAST of Clc05G22100 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 4.8e-135
Identity = 212/347 (61.10%), Postives = 265/347 (76.37%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYGT+ ELKSLI A H K ++ +ADIVINHR A+ +D RG++C+FEGGTP
Sbjct: 78  PGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTP 137

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDWGP  ICSDDT+YS+G+G+ D+G  +  APDIDHLNP+VQ+E+++W+ WL+T++G
Sbjct: 138 DGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLG 197

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWR DF KGY+  + ++Y+D T P F VGE W  L    D KP+ NQDA R  L NWV
Sbjct: 198 FDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWV 257

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
              G   TAFDFTTKGILQAAVQGELWRL D NGK PGL+G  P+ AVTF+DNHDTGSTQ
Sbjct: 258 EGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQ 317

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
            +WPFPSDKV+QGYAYILTHPG P IFYDH  DW L+ EI  L  IR+RNGI   STL+I
Sbjct: 318 SLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDI 377

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
           + ++GD+YVA +DGK+I K+G   + G +IPSD+ +   G +Y  W+
Sbjct: 378 LKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424

BLAST of Clc05G22100 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 481.5 bits (1238), Expect = 1.8e-134
Identity = 214/347 (61.67%), Postives = 261/347 (75.22%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYGT  ELKSLIAA H KG++ +AD+VINHR AEK+D RG++C+FEGGTP
Sbjct: 78  PGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTP 137

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDWGP  ICSDDT+YSDG G+ D+GE +  APDIDHLNP+VQ+E+++W+ WLK+++G
Sbjct: 138 DDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWLKSDVG 197

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWR DF KGY+  I K+Y++   P F V E W+ LS   D KP  NQD  R  L NWV
Sbjct: 198 FDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGDGKPAANQDQGRQELVNWV 257

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
            A G     FDFTTKG+LQA VQGELWRLRD NGK  G+IG  PE AVTF+DNHDTGSTQ
Sbjct: 258 NAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWLPEKAVTFVDNHDTGSTQ 317

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
           ++WPFPSDKV+QGYAYILTHPG P IFYDH  DW LK+EI  L AIR RNGI   S L I
Sbjct: 318 KLWPFPSDKVMQGYAYILTHPGVPCIFYDHMFDWNLKQEITALAAIRERNGINAGSKLRI 377

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
           + +  D YVA VD K+++KIG   ++GN +PSD+     G +Y+ W+
Sbjct: 378 VVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTVHGKDYSVWE 424

BLAST of Clc05G22100 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 3.1e-134
Identity = 223/348 (64.08%), Postives = 278/348 (79.89%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDLN+SKYG++ ELKSLI AL+QKGIKA+ADIVINHRTAE++D +  +C FEGGT 
Sbjct: 78  PGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTS 137

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+VQKE+SEWM WLKTEIG
Sbjct: 138 DDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIG 197

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           F GWRFD+V+GYA SITKLY+  T PDFAVGEKWD++  G D K + +Q+ HR  L  W+
Sbjct: 198 FHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWI 257

Query: 188 -RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGST 247
             AGG  +TAFDFTTKGILQ+AV+GELWRL+DS GKPPG+IGI P +AVTFIDNHD   T
Sbjct: 258 EEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---T 317

Query: 248 QRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLN 307
            R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE I KL AIR +NGIG TS++ 
Sbjct: 318 FRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVT 377

Query: 308 IMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
           I A++ DLY+A +D K+IMKIG   ++G L+PS++ LA SG+++A W+
Sbjct: 378 IKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421

BLAST of Clc05G22100 vs. ExPASy TrEMBL
Match: A0A6A5NSP1 (Alpha-amylase OS=Lupinus albus OX=3870 GN=Lal_00045845 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 8.7e-310
Identity = 519/797 (65.12%), Postives = 607/797 (76.16%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYG++ ELKSLIAALH KGIKA+ADIVINHRTAE++D+RGI+ IFEGGT 
Sbjct: 75  PGRLYDLDASKYGSKEELKSLIAALHSKGIKAVADIVINHRTAERKDERGIYSIFEGGTS 134

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D +LDWGP FIC DDT YSDG GN DSGE Y  APDIDHLNPQ QKE+SEWM WLKTE+G
Sbjct: 135 DGKLDWGPHFICKDDTAYSDGTGNLDSGEGYAAAPDIDHLNPQAQKELSEWMNWLKTEVG 194

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWRFDFVKGYAPSITK+YM++T PDFAVGE W+ LS  QD KP++NQD HR AL NWV
Sbjct: 195 FDGWRFDFVKGYAPSITKIYMEKTSPDFAVGENWNSLSY-QDGKPDKNQDGHRGALVNWV 254

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
              G  V AFDFTTKGILQAAV+GELWRL+DSNGKP GLIG+KPE+AVTFIDNHDTGSTQ
Sbjct: 255 EGAGGVVNAFDFTTKGILQAAVEGELWRLKDSNGKPSGLIGVKPENAVTFIDNHDTGSTQ 314

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSI-------------------FYDHFVDWGLKEEIM 307
             WPFPSDKV+QGYAYILTHPGTPSI                   FYDHF +WGLKEEI 
Sbjct: 315 NSWPFPSDKVMQGYAYILTHPGTPSIFIGARILSGIRVYNTLECEFYDHFFNWGLKEEIA 374

Query: 308 KLNAIRARNGIGKTSTLNIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGM 367
           KL AIR +NGI   S+++I+A+  DLYVA +D KII+KIG   ++GNLIPS++ +A SG 
Sbjct: 375 KLTAIRLKNGINSKSSVDILAADADLYVAKIDNKIIVKIGPKMDVGNLIPSNFHVAASGK 434

Query: 368 EYAAWKLLIQQKAMASPSPWCFFLLLALVFPSSTTTQILFQGFNW-DSKSSAGWYNILKT 427
            YA W                             T   +  GFNW  S    GWYN LKT
Sbjct: 435 NYAVW--------------------------LEYTKMNMCMGFNWASSDKEGGWYNFLKT 494

Query: 428 KIPDLVNAGITHVWLPPPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGI 487
            +PD+ +AG+ +VWLPP SNSH +G +GYLP RLYDLDTSKYGNK+E K+LI      G+
Sbjct: 495 LVPDIADAGVNYVWLPPLSNSHDDGPQGYLPKRLYDLDTSKYGNKEESKSLIDAFSERGV 554

Query: 488 KAIADIVINHRTAERQDSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFSGDGNPDTGMDW 547
           K+++DIVINHRTAER D+NG  SIFEGGTPDNRLDWN+S++C ND+ F+G GN DTG DW
Sbjct: 555 KSMSDIVINHRTAERLDNNG-LSIFEGGTPDNRLDWNVSYICGNDTRFNGTGNLDTGDDW 614

Query: 548 GEAPDIDHLNPQVQTELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVG 607
             APDIDH+NP VQ ELSDWMNWLK++VGF+GWRFDMVVGY P  TKIYME+TSP+FAVG
Sbjct: 615 WGAPDIDHVNPNVQKELSDWMNWLKTEVGFVGWRFDMVVGYAPRFTKIYMEQTSPDFAVG 674

Query: 608 EKWDN----PPGRVVQEQD--RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKD 667
           E + N      GR +  QD  R  L+ W+  AGG V AFDFTTK VL AAV+GELWRLKD
Sbjct: 675 ELYRNVTRGSDGRPLANQDEHRSTLVNWVNDAGGVVTAFDFTTKMVLGAAVQGELWRLKD 734

Query: 668 SSNRPPGLIGLKPENAVTFIDNHDTFSQRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLL 727
            + +PPG+IG KPENAVTF+DNHDT SQ++ PFP  KVMLGYVYILTHPG PTIFYDH +
Sbjct: 735 DNGKPPGMIGTKPENAVTFVDNHDTLSQQTWPFPNDKVMLGYVYILTHPGHPTIFYDHYI 794

Query: 728 EWGQTLQKPIVELTTVRKRIGINNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLL 779
           EWG  L++PI  +T +RKR GIN KS V I+ AEADLYMAEI+ K+IVKIG + +LGNLL
Sbjct: 795 EWG--LKEPIKNVTEIRKRNGINPKSTVNILAAEADLYMAEIDNKIIVKIGPKEDLGNLL 839

BLAST of Clc05G22100 vs. ExPASy TrEMBL
Match: A0A1R3I8V0 (1,4-alpha-D-glucan glucanohydrolase OS=Corchorus capsularis OX=210143 GN=CCACVL1_14000 PE=4 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 2.4e-299
Identity = 498/759 (65.61%), Postives = 586/759 (77.21%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDL+ASKYG+Q ELKSLI A HQ G+K +ADIVINHRTAE +D RGI+ IFEGGT 
Sbjct: 187 PGRLYDLDASKYGSQAELKSLIDAFHQNGVKCVADIVINHRTAESKDSRGIYSIFEGGTS 246

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDWGPSFICSDD EYSDG GNPD+G PY PAPDIDHLNP+VQKE+S+WM WLKTEIG
Sbjct: 247 DDRLDWGPSFICSDDKEYSDGTGNPDTGLPYNPAPDIDHLNPRVQKELSDWMNWLKTEIG 306

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           FDGWRFDFV+GYAPSITK+YMDQT PDFAVGEKW++ S GQ       +D+HR AL +WV
Sbjct: 307 FDGWRFDFVRGYAPSITKIYMDQTSPDFAVGEKWEDFSLGQ-------EDSHRSALKDWV 366

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
            A G  VTAFDFTTKG+L AAVQG L RL+DSNGKPPGLIG+ P++AVTFIDNHDTGSTQ
Sbjct: 367 EAAGGAVTAFDFTTKGVLNAAVQGALGRLKDSNGKPPGLIGLLPQNAVTFIDNHDTGSTQ 426

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
            +WPFPSDKV+QGYAYILTHPG PSIFYDHF DW LK+EI KL AIR  NGI  TST+NI
Sbjct: 427 NLWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWDLKDEISKLVAIRNSNGINPTSTVNI 486

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMASPSP 367
           +AS  DLYVA +D KIIMKIG  +++GNL+PS+YQLA SG +Y+                
Sbjct: 487 LASDADLYVASIDDKIIMKIGPRSDVGNLVPSNYQLAASGKDYS---------------- 546

Query: 368 WCFFLLLALVFPSSTTTQILFQGFNWDS-KSSAGWYNILKTKIPDLVNAGITHVWLPPPS 427
                                 GFNW+S     GWYN LK  +PD+ NAG+THVW PPPS
Sbjct: 547 ----------------------GFNWESCNKGGGWYNSLKNFVPDIANAGVTHVWFPPPS 606

Query: 428 NSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQDSN 487
            S   G +GYLPGRLYDLD SKYG+K ELK+LI    + GIK +ADIVINHRTAER+D  
Sbjct: 607 QS--VGPQGYLPGRLYDLDASKYGSKAELKSLIEAFHQKGIKCLADIVINHRTAERKDGR 666

Query: 488 GRWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDIDHLNPQVQTELS 547
           G +SIFEGGTPD+RLDW  SF+C ND  +S G GNPDTG+D+  APDIDHLNP+VQ+ELS
Sbjct: 667 GIYSIFEGGTPDDRLDWGPSFICGNDREYSDGSGNPDTGLDYQPAPDIDHLNPRVQSELS 726

Query: 548 DWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKW-DNPPGRVVQEQDRR 607
           DWMNWLK+++GF GWRFD V GY P  TKIYMERTSP+FAVGEKW D   G+  Q+  R 
Sbjct: 727 DWMNWLKTEIGFDGWRFDFVRGYAPSITKIYMERTSPDFAVGEKWEDLSLGQGGQDWHRG 786

Query: 608 ALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPENAVTFIDNH 667
           +L +W++AAGG V AFDFTTK VL+AAV+GELWRLKDS+ +PPG+IGL P+NAVTFIDNH
Sbjct: 787 SLKDWVEAAGGVVKAFDFTTKGVLNAAVQGELWRLKDSNGKPPGMIGLLPQNAVTFIDNH 846

Query: 668 DTFS-QRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTLQKPIVELTTVRKRIGI 727
           DT S QR  PFP  KVM GY YILTHPGTP+IFYDH ++WG  L+  I +L  +R + GI
Sbjct: 847 DTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFIDWG--LKDEITKLAVIRNKNGI 896

Query: 728 NNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLLP 763
           +  S  RI+ +++DLYMA I+EK+I+KIG +++LGNLLP
Sbjct: 907 SATSTARILASDSDLYMAAIDEKIIMKIGPKMDLGNLLP 896

BLAST of Clc05G22100 vs. ExPASy TrEMBL
Match: A0A498K3I6 (1,4-alpha-D-glucan glucanohydrolase OS=Malus domestica OX=3750 GN=DVH24_021633 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 9.9e-293
Identity = 496/855 (58.01%), Postives = 604/855 (70.64%), Query Frame = 0

Query: 8    PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
            P +LYDLNAS+YG Q+ELK+LI   H  GI++IADIVINHR AEK+D+RGIWCIFEGGTP
Sbjct: 385  PGRLYDLNASRYGNQDELKALINTFHDNGIQSIADIVINHRCAEKKDERGIWCIFEGGTP 444

Query: 68   DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
            D RLDWGPS ICSDDT YS+GKGNPD+G  +  APDIDH+N +VQ+E+S+WM WLKTEIG
Sbjct: 445  DDRLDWGPSLICSDDTAYSNGKGNPDTGADFSAAPDIDHINTRVQRELSDWMNWLKTEIG 504

Query: 128  FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
            F+GWRFDFVKGYAP  TKL++  T P F+VGE W+ L+ G D K   NQDAHR AL  WV
Sbjct: 505  FNGWRFDFVKGYAPEFTKLFVTNTRPSFSVGELWNSLAYGSDGKLEYNQDAHRRALVGWV 564

Query: 188  RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
               G  VTAFDFTTKGILQAAVQGELWR++DSNG  PG+IG+ P  +VTF+DNHDTGSTQ
Sbjct: 565  EGAGGDVTAFDFTTKGILQAAVQGELWRMKDSNGGAPGMIGLSPGKSVTFVDNHDTGSTQ 624

Query: 248  RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
             MWPFPSDKV+QGYAYILTHPG PSIFYDH+ DWGLKEEI KL AIR RNG+G  S L I
Sbjct: 625  NMWPFPSDKVMQGYAYILTHPGIPSIFYDHYFDWGLKEEITKLVAIRLRNGVGPDSALRI 684

Query: 308  MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK------------- 367
            +AS  DLYVA  D KII KIG   ++GNL+P  YQ+ATSG +Y  W+             
Sbjct: 685  LASDADLYVAATDEKIIAKIGPRYDVGNLVPPTYQIATSGNDYCVWEKKAGALFSGSNNL 744

Query: 368  ---------------LLIQQKAMASP---------------------------------- 427
                           LL +  +M +P                                  
Sbjct: 745  AEVRTVLMLPDLIQDLLQRFLSMENPVKPSLLAISKSFPLTRRPITARHVSTSKANHSAT 804

Query: 428  ----------SPWCFFLLLALVFPSSTTTQILFQGFNWDSKSSAGWYNILKTKIPDLVNA 487
                       P   FLL  L+ P+ T +QI+FQGFNW+S    GWYNILK  + DL ++
Sbjct: 805  RLKGDMNTRNVPLICFLLSILLTPNFTASQIIFQGFNWESSRKGGWYNILKQSVQDLASS 864

Query: 488  GITHVWLPPPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVI 547
            GITHVWLPP  +SH    EGY+PGRLYDL+ SK+GNKDELK+L++  R +GI+++ADIVI
Sbjct: 865  GITHVWLPP--SSHAASDEGYMPGRLYDLN-SKFGNKDELKSLVSAYRNSGIQSVADIVI 924

Query: 548  NHRTAERQDSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDID 607
            NHRTAE+QD  G W IFEGGT D+RLDW  S +C +D+ +S G GNPDTG D+G APDID
Sbjct: 925  NHRTAEKQDERGIWCIFEGGTDDDRLDWGPSLICGDDTKYSDGKGNPDTGEDFGGAPDID 984

Query: 608  HLNPQVQTELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDN-- 667
            H N +VQ ELSDWMNWLK+++GF GWRFD V GY P  TK+YM  TSP FAVGE W++  
Sbjct: 985  HKNTRVQKELSDWMNWLKTEIGFSGWRFDFVKGYAPEYTKLYMTNTSPNFAVGELWNSLS 1044

Query: 668  --PPGRVVQEQD--RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPG 727
                G+   +QD  R+AL+ W+++AGG V AFDFTTK +L AAV+GELWRLKDS+   PG
Sbjct: 1045 YGSDGKPNPDQDAHRQALVGWVESAGGGVTAFDFTTKGILQAAVQGELWRLKDSNGGAPG 1104

Query: 728  LIGLKPENAVTFIDNHDTFS-QRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTL 783
            +IGLKP ++VTFIDNHDT S Q   PFP  KVM GY YILTHPG P+IFYDH  +WG  L
Sbjct: 1105 MIGLKPGSSVTFIDNHDTGSTQHLWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWG--L 1164

BLAST of Clc05G22100 vs. ExPASy TrEMBL
Match: A0A5N5IDQ0 (Alpha-amylase OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_026374 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 1.0e-273
Identity = 463/783 (59.13%), Postives = 555/783 (70.88%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDLNAS+YG Q+ELK+LI   H  GI++IADIVINHR AEK+D+RGIWCIFEGGTP
Sbjct: 78  PGRLYDLNASRYGNQDELKALINTFHDNGIQSIADIVINHRCAEKKDERGIWCIFEGGTP 137

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDWGPS ICSDD  YS+GKGNPD+G  +  APDIDH+N +VQ+E+S+WM WLKTEIG
Sbjct: 138 DDRLDWGPSLICSDDIAYSNGKGNPDTGADFSAAPDIDHINTRVQRELSDWMNWLKTEIG 197

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           F GWRFDFVKGYAP  TKL++ +T P F+VGE W+ L+ G D K   NQDAHR AL  WV
Sbjct: 198 FSGWRFDFVKGYAPEFTKLFVTKTRPSFSVGELWNSLAYGSDGKLEYNQDAHRRALVGWV 257

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
              G  VTAFDFTTKGILQAAVQGELWR++DSNG  PG+IG+ P  +VTFIDNHDTGSTQ
Sbjct: 258 EGAGGDVTAFDFTTKGILQAAVQGELWRMKDSNGGAPGMIGLSPGKSVTFIDNHDTGSTQ 317

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLNI 307
            MWPFPSDKV+QGYAYILTHPG PSIFYDHF DWGLKEEI KL AIR RNGIG  S L I
Sbjct: 318 NMWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWGLKEEITKLVAIRLRNGIGPDSALRI 377

Query: 308 MASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMASPSP 367
           +AS  DLYVA +D KII KIG                                       
Sbjct: 378 LASDADLYVAAIDEKIIAKIGPR------------------------------------- 437

Query: 368 WCFFLLLALVFPSSTTTQILFQGFNWDSKSSAGWYNILKTKIPDLVNAGITHVWLPPPSN 427
                                           GWYNILK  + DL ++GITHVWLPP  +
Sbjct: 438 ------------------------------KGGWYNILKQSVQDLASSGITHVWLPP--S 497

Query: 428 SHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQDSNG 487
           S     EGY+PGRLYDL+T KYGNKDELK+L++  R +GI+++ADIVINHRTAE+QD  G
Sbjct: 498 SQAASDEGYMPGRLYDLNT-KYGNKDELKSLVSAYRDSGIQSVADIVINHRTAEKQDERG 557

Query: 488 RWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDIDHLNPQVQTELSD 547
            W IFEGGT D+RLDW  S +C +D+ +S G GNPDTG D+G APDIDH N +VQ ELSD
Sbjct: 558 IWCIFEGGTDDDRLDWGPSLICSDDTKYSDGKGNPDTGEDFGGAPDIDHKNTRVQKELSD 617

Query: 548 WMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDN----PPGRVVQEQD 607
           WMNWLK+++GF GWRFD V GY P  TK+YM  TSP FAVGE W++      G+   +QD
Sbjct: 618 WMNWLKTEIGFSGWRFDFVKGYAPEYTKLYMTNTSPNFAVGELWNSLSYGSDGKPNPDQD 677

Query: 608 --RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPENAVTF 667
             R+AL+ W+++AGG V AFDFTTK +L AAV+GELWRLKDS     G+IG+KP N+VTF
Sbjct: 678 AHRQALVRWVESAGGRVTAFDFTTKGILQAAVQGELWRLKDSKGGASGMIGVKPGNSVTF 737

Query: 668 IDNHDTFS-QRSAPFPEGKVMLGYVYILTHPGTPTIFYDHLLEWGQTLQKPIVELTTVRK 727
           IDNHDT S Q   PFP  KVM GY YILTHPG P+IFYDH  +WG  L++ I ++T +R 
Sbjct: 738 IDNHDTGSTQHMWPFPSDKVMQGYAYILTHPGIPSIFYDHYFDWG--LKEEISKITAIRS 786

Query: 728 RIGINNKSKVRIITAEADLYMAEIEEKMIVKIGGRLNLGNLLPPTYREIATISGEDYAVW 783
           R GI   S +RI+ A+AD+Y+A I+EK+IVKIG +++LG L+P  ++   + SG+DYAVW
Sbjct: 798 RNGIKPNSALRILAADADVYVASIDEKIIVKIGPKMDLGQLIPTNFQ--VSTSGKDYAVW 786

BLAST of Clc05G22100 vs. ExPASy TrEMBL
Match: A0A6A6MBE3 (1,4-alpha-D-glucan glucanohydrolase OS=Hevea brasiliensis OX=3981 GN=GH714_005263 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 1.3e-271
Identity = 479/813 (58.92%), Postives = 577/813 (70.97%), Query Frame = 0

Query: 6   HPPNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGG 65
           + P +LYDL  SKYG+Q+ELKSLIAA HQ GIK +ADIVINHR+AEKQD+RG W IFEGG
Sbjct: 214 YKPGRLYDL-TSKYGSQDELKSLIAAFHQNGIKCVADIVINHRSAEKQDERGAWSIFEGG 273

Query: 66  TPDSRLDWGPSFICS-DDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKT 125
           T D+RLDW  SFICS DDT YS+GKGNPD+G  +    DIDHLNP+VQKE+S+WM WLK+
Sbjct: 274 TSDNRLDWDASFICSNDDTGYSNGKGNPDTGMDFPNVTDIDHLNPRVQKELSDWMNWLKS 333

Query: 126 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALA 185
           EIGFDGWR DFVKG++ +I K+YM+QT P+FAVGE WD  S GQD KP  + +AHR AL 
Sbjct: 334 EIGFDGWRLDFVKGFSTNIAKIYMEQTKPNFAVGEYWDFFSYGQDGKPVTDLNAHRRALR 393

Query: 186 NWVRAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTG 245
           +WV+A G+ VTAFDFTTKG LQAAVQGELWRL D++GKPPG IGI PE+AVTFIDNHDT 
Sbjct: 394 DWVQAVGNGVTAFDFTTKGALQAAVQGELWRLNDTSGKPPGFIGISPENAVTFIDNHDTW 453

Query: 246 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTST 305
           S Q++WPFPSDKV+ GY YILTHPGTPSI           E I K+ +IR   GI +TST
Sbjct: 454 S-QQVWPFPSDKVMLGYVYILTHPGTPSI-----------EPISKVASIRKIYGISETST 513

Query: 306 LNIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWKLLIQQKAMAS 365
           + I+A++ DLY+A ++  +IMKIG   +LGNL+PS+++LAT G +YA W           
Sbjct: 514 VKILAAESDLYMAMINDNVIMKIGPKLDLGNLLPSNFELATPGQDYAVW----------- 573

Query: 366 PSPWCFFLLLALVFPSSTTTQILFQGFNWDS-KSSAGWYNILKTKIPDLVNAGITHVWLP 425
                                    GFNW+S     GWYN LK  IPDLVNAGITHVWLP
Sbjct: 574 -------------------------GFNWESCNKEGGWYNSLKNSIPDLVNAGITHVWLP 633

Query: 426 PPSNSHPNGSEGYLPGRLYDLDTSKYGNKDELKALIADLRRNGIKAIADIVINHRTAERQ 485
           PPS S     EGY+PGRLYDL  S YG +DELK+LI    + GIK +ADIVINHRTAE+Q
Sbjct: 634 PPSQS--VSCEGYMPGRLYDLKASTYGTQDELKSLIQAFHQKGIKCLADIVINHRTAEKQ 693

Query: 486 DSNGRWSIFEGGTPDNRLDWNISFVCKNDSPFS-GDGNPDTGMDWGEAPDIDHLNPQVQT 545
           D+ G W IFEGGT D+RLDW  SF+C +D+ +S G GNPDTG D+  APDIDHLN +VQ 
Sbjct: 694 DNRGIWCIFEGGTSDDRLDWGPSFICSDDTAYSDGKGNPDTGDDFAPAPDIDHLNLRVQK 753

Query: 546 ELSDWMNWLKSDVGFIGWRFDMVVGYTPMATKIYMERTSPEFAVGEKWDN----PPGRVV 605
           ELSDWMNWL S++GF GWRFD V GY P  TKIYME+TSP FAV EKWD+      G+  
Sbjct: 754 ELSDWMNWLNSEIGFDGWRFDFVKGYAPSITKIYMEKTSPNFAVSEKWDSLAYGQDGKPD 813

Query: 606 QEQD--RRALLEWIQAAGGAVAAFDFTTKFVLHAAVKGELWRLKDSSNRPPGLIGLKPEN 665
             QD  R AL +WIQAAGG   AFDFTTK +L AAV+GELWRLKDS+ +PPGLIG+ P+N
Sbjct: 814 ANQDAHRGALKDWIQAAGGITTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGILPQN 873

Query: 666 AVTFIDNHDTFS-QRSAPFPEGKVMLGYVYILTHPGTPTI-------------------- 725
           AVTFIDNHDT S QR  PFP  KVM GY YILTHPG P+I                    
Sbjct: 874 AVTFIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGIPSIVTSAQSFQFYFSCDSNCTKR 933

Query: 726 ------FYDHLLEWGQTLQKPIVELTTVRKRIGINNKSKVRIITAEADLYMAEIEEKMIV 783
                 FYDH  +WG  L++ I +L ++RK  GI+++S V+I+ A++DLYMA I + +I+
Sbjct: 934 CFLPLQFYDHFFDWG--LKEQISKLASIRKTYGIHDRSTVKILAAQSDLYMATINDNIIM 971

BLAST of Clc05G22100 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 480.7 bits (1236), Expect = 2.2e-135
Identity = 223/348 (64.08%), Postives = 278/348 (79.89%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P +LYDLN+SKYG++ ELKSLI AL+QKGIKA+ADIVINHRTAE++D +  +C FEGGT 
Sbjct: 78  PGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTS 137

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
           D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+VQKE+SEWM WLKTEIG
Sbjct: 138 DDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIG 197

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           F GWRFD+V+GYA SITKLY+  T PDFAVGEKWD++  G D K + +Q+ HR  L  W+
Sbjct: 198 FHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWI 257

Query: 188 -RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGST 247
             AGG  +TAFDFTTKGILQ+AV+GELWRL+DS GKPPG+IGI P +AVTFIDNHD   T
Sbjct: 258 EEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---T 317

Query: 248 QRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLNAIRARNGIGKTSTLN 307
            R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE I KL AIR +NGIG TS++ 
Sbjct: 318 FRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVT 377

Query: 308 IMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
           I A++ DLY+A +D K+IMKIG   ++G L+PS++ LA SG+++A W+
Sbjct: 378 IKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421

BLAST of Clc05G22100 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 313.5 bits (802), Expect = 4.7e-85
Identity = 160/349 (45.85%), Postives = 215/349 (61.60%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P  LY+LN S+YGT +ELK  +   H+ GIK + D V+NHR A  ++Q G+W +F G   
Sbjct: 547 PKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG--- 606

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
             RL+W    + +DD  +  G+GN  SG+ +  AP+IDH    V+K+I EW+ W+  E+G
Sbjct: 607 --RLNWDDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVG 666

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           +DGWR DFV+G+     K YMD + P FAVGE WD LS     + + NQDAHR  + +W+
Sbjct: 667 YDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG-EMDYNQDAHRQRIVDWI 726

Query: 188 RAGGDTVTAFDFTTKGILQAAVQG-ELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGST 247
            A      AFD TTKGIL  A+Q  E WRL D  GKPPG++G  P  AVTFI+NHDTGST
Sbjct: 727 NATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGST 786

Query: 248 QRMWPFPSDKVIQGYAYILTHPGTPSIFYDH-FVDWGLKEEIMKLNAIRARNGIGKTSTL 307
           Q  W FP  K +QGYAYILTHPGTP++F+DH F D+    EI  L ++R R  +   S +
Sbjct: 787 QGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDY--HSEIAALLSLRNRQKLHCRSEV 846

Query: 308 NIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAAWK 355
           NI  S+ D+Y A +D K+ MKIG           ++ +A  G +Y  W+
Sbjct: 847 NIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of Clc05G22100 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 310.8 bits (795), Expect = 3.0e-84
Identity = 159/350 (45.43%), Postives = 218/350 (62.29%), Query Frame = 0

Query: 8   PNQLYDLNASKYGTQNELKSLIAALHQKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTP 67
           P  LY LN S YG+++ LKSL+  + Q  ++A+ADIVINHR    +   G++  ++G   
Sbjct: 76  PQDLYSLN-SAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG--- 135

Query: 68  DSRLDWGPSFICSDDTEYSDGKGNPDSGEPYLPAPDIDHLNPQVQKEISEWMRWLKTEIG 127
              L W    +    T  + G GN  +G+ +   P++DH    V+K+I  W+RWL+  +G
Sbjct: 136 -ISLPWDEHAV----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVG 195

Query: 128 FDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDELSEGQDRKPNQNQDAHRDALANWV 187
           F  +RFDF +GY+ +  K Y+    P F+VGE WD  +       + NQD+HR  + +W+
Sbjct: 196 FQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNY-NGHGLDYNQDSHRQRIISWI 255

Query: 188 RAGGDTVTAFDFTTKGILQAAVQGELWRLRDSNGKPPGLIGIKPESAVTFIDNHDTGSTQ 247
            A G    AFDFTTKGILQ AV+G+ WRL D+ GKPPG++G  P  AVTF+DNHDTGSTQ
Sbjct: 256 DATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQ 315

Query: 248 RMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWG--LKEEIMKLNAIRARNGIGKTSTL 307
             WPFPS  V++GYAYILTHPG P +FYDHF DWG  + ++I+KL  IR R  I   ST+
Sbjct: 316 AHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTV 375

Query: 308 NIMASQGDLYVAGVDGKIIMKIGANTNLGNLIPS--DYQLATSGMEYAAW 354
            ++ ++ +LY A V  KI MK+G     G+  PS  D+ LATSG  YA W
Sbjct: 376 RVLKAESNLYAAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVW 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAF1886612.11.8e-30965.12hypothetical protein Lal_00045845 [Lupinus albus][more]
OMO78941.15.0e-29965.61hypothetical protein CCACVL1_14000 [Corchorus capsularis][more]
RXH99831.12.0e-29258.01hypothetical protein DVH24_021633 [Malus domestica][more]
KAB2635840.12.1e-27359.13alpha-amylase-like [Pyrus ussuriensis x Pyrus communis][more]
KAF2309818.12.6e-27158.92hypothetical protein GH714_005263 [Hevea brasiliensis][more]
Match NameE-valueIdentityDescription
P178591.6e-16777.52Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1[more]
P040636.7e-13763.90Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3[more]
P279344.8e-13561.10Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... [more]
P279331.8e-13461.67Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2... [more]
Q8VZ563.1e-13464.08Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6A5NSP18.7e-31065.12Alpha-amylase OS=Lupinus albus OX=3870 GN=Lal_00045845 PE=3 SV=1[more]
A0A1R3I8V02.4e-29965.611,4-alpha-D-glucan glucanohydrolase OS=Corchorus capsularis OX=210143 GN=CCACVL1... [more]
A0A498K3I69.9e-29358.011,4-alpha-D-glucan glucanohydrolase OS=Malus domestica OX=3750 GN=DVH24_021633 P... [more]
A0A5N5IDQ01.0e-27359.13Alpha-amylase OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_026374 P... [more]
A0A6A6MBE31.3e-27158.921,4-alpha-D-glucan glucanohydrolase OS=Hevea brasiliensis OX=3981 GN=GH714_00526... [more]
Match NameE-valueIdentityDescription
AT4G25000.12.2e-13564.08alpha-amylase-like [more]
AT1G69830.14.7e-8545.85alpha-amylase-like 3 [more]
AT1G76130.13.0e-8445.43alpha-amylase-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006046Alpha amylasePRINTSPR00110ALPHAAMYLASEcoord: 232..244
score: 51.43
coord: 36..47
score: 49.51
coord: 128..139
score: 40.85
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 719..781
e-value: 7.5E-23
score: 91.9
coord: 295..355
e-value: 5.4E-25
score: 99.0
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 721..780
e-value: 7.1E-15
score: 55.0
coord: 296..354
e-value: 6.0E-14
score: 52.1
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 2..294
e-value: 1.7E-26
score: 104.0
coord: 385..730
e-value: 6.1E-41
score: 152.0
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 17..141
e-value: 9.5E-12
score: 45.1
coord: 408..697
e-value: 4.1E-14
score: 52.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 384..722
e-value: 1.1E-121
score: 408.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 6..298
e-value: 3.3E-120
score: 403.6
NoneNo IPR availablePIRSRPIRSR001021-2PIRSR001021-2coord: 88..301
e-value: 4.5E-16
score: 55.2
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 367..782
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 8..354
NoneNo IPR availablePANTHERPTHR43447:SF37ALPHA-AMYLASE 1coord: 8..354
NoneNo IPR availablePANTHERPTHR43447:SF37ALPHA-AMYLASE 1coord: 367..782
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 8..305
e-value: 1.02722E-133
score: 396.977
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 386..729
e-value: 5.31039E-140
score: 413.155
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 723..781
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 299..354
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 723..782
e-value: 1.7E-18
score: 68.5
coord: 299..355
e-value: 2.0E-18
score: 68.3
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 13..285
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 382..705

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc05G22100.2Clc05G22100.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0043169 cation binding