Homology
BLAST of Clc03G01130 vs. NCBI nr
Match:
XP_038892835.1 (neutral ceramidase 2-like [Benincasa hispida])
HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 759/781 (97.18%), Postives = 772/781 (98.85%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLFDL RRSLG+IWLCIY+LVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFDLTVRRSLGTIWLCIYMLVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANAEQIASGVHFRL ARAFIVAEPQGKRV+FVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVIFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWFEQKG T +HPGESE DSIPRRVSNIIPEV+KN
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGT-MHPGESEVDSIPRRVSNIIPEVFKN 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
KQELLERAASFQS+PGRPATRI+SVSRRVRNVLRQA+RPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 KQELLERAASFQSQPGRPATRILSVSRRVRNVLRQANRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQLT
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLT 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
GKVD+RHSYVDFSQLEVSL+KQGGATEV++TCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GKVDFRHSYVDFSQLEVSLSKQGGATEVIRTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS
Sbjct: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVPLNSTFKRGNLVTV+FWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF
Sbjct: 661 FGDVKSDVPLNSTFKRGNLVTVSFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. NCBI nr
Match:
KAG6583392.1 (Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1494.2 bits (3867), Expect = 0.0e+00
Identity = 738/781 (94.49%), Postives = 759/781 (97.18%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD RRSLGSIWLC+Y+LV+LQSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDHTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RYKYDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKG+ T L G+SEADSIPRRVSNIIPE YK+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGT-LQTGKSEADSIPRRVSNIIPEAYKD 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
+QELL RAASFQSRPGRPATRI+SVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 EQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQL
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLK 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
G VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV+MDATP GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVPLNSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDF
Sbjct: 661 FGDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. NCBI nr
Match:
KAG7019159.1 (Neutral ceramidase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 737/781 (94.37%), Postives = 759/781 (97.18%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD RRSLGSIWLC+Y+LV+LQSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RYKYDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKG+ T L G+SEADSIPRRVSNIIPE Y++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGT-LQTGKSEADSIPRRVSNIIPEAYRD 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
+QELL RAASFQSRPGRPATRI+SVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 EQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQL
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLK 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
G VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV+MDATP GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVPLNSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDF
Sbjct: 661 FGDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. NCBI nr
Match:
XP_022964852.1 (neutral ceramidase 2-like [Cucurbita moschata])
HSP 1 Score: 1491.5 bits (3860), Expect = 0.0e+00
Identity = 736/781 (94.24%), Postives = 758/781 (97.06%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD RRSLGSIWLC+Y+LV+LQSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RYKYDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKG+ T L G+SEADSIPRRVSNIIPE Y +
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGT-LQTGKSEADSIPRRVSNIIPEAYND 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
+QELL RAASFQSRPGRPATRI+SVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 EQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQL
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLK 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
G VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV+MDATP GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDV+SDVPLNSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDF
Sbjct: 661 FGDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. NCBI nr
Match:
XP_022970515.1 (neutral ceramidase 2-like [Cucurbita maxima])
HSP 1 Score: 1486.5 bits (3847), Expect = 0.0e+00
Identity = 735/781 (94.11%), Postives = 755/781 (96.67%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD RRSLGSIWLC+Y+LV+LQSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQGK+VVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKQVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDA VNRSPSAYLNNPASER RYKYDVDKEMTLLKFVDD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDASVNRSPSAYLNNPASERDRYKYDVDKEMTLLKFVDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKG+RT L G+SEADSIPRRVSNIIPE YK+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIRT-LQTGKSEADSIPRRVSNIIPEAYKD 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
+QELLERAA+FQSRPGRPATRI+SVSRRVRN LRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 EQELLERAAAFQSRPGRPATRILSVSRRVRNALRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQL
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLK 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
G VDYRHSYVDFSQLEVS+TK GGAT VVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GTVDYRHSYVDFSQLEVSVTKPGGATAVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLK PG+EQIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKTPGSEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV+MDATP GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVPLNSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDF
Sbjct: 661 FGDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKW RPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK+LL SIRHFTGSSSAFVV
Sbjct: 721 CLRFKWWRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKNLLRSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. ExPASy Swiss-Prot
Match:
F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)
HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 576/761 (75.69%), Postives = 647/761 (85.02%), Query Frame = 0
Query: 22 IYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAFI 81
I ++ L + V S S+YLIGLGS+DITGPAADVNMMGYAN EQ+ASG+HFRL AR FI
Sbjct: 19 ISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFI 78
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
V+EPQGKRVVFVNLDACMASQIV +KV+ERLKARYGDLYTE+NV ISGIHTHAGPGGYLQ
Sbjct: 79 VSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQ 138
Query: 142 YVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YVVYIVTSLGFVRQSFD LVDGIE SIIQAHENL PGSI +N GEL+DAGVNRSPSAYLN
Sbjct: 139 YVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLN 198
Query: 202 NPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NP+ ERS++KY+VDKEMTLLKFVDD+WGPVGSFNWFATHGTSMSRTNSLISGDNKGAA+R
Sbjct: 199 NPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASR 258
Query: 262 FMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQSRPGRPATR 321
FMEDW+EQ + E +D IPRRVS++I + ELLE A+ F+S+PG+P TR
Sbjct: 259 FMEDWYEQNTAER-SYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTR 318
Query: 322 IVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNE 381
I S +RRVR+ LR+AD+P FVSAFCQ+NCGDVSPN LGAFC+DTGLPCDFNHSTCGGKNE
Sbjct: 319 ISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNE 378
Query: 382 LCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTK 441
+CYGRGPGYPDEFEST IIGERQF+ A++LF+KASEQL GKVDYRH YVDFSQL V+L K
Sbjct: 379 MCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPK 438
Query: 442 QGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIAC 501
+ G +EVVKTCPAAMGFAFAAGTTDGPGAFDF QGDDKGN FW+LVRN+LK P +QI C
Sbjct: 439 KDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDC 498
Query: 502 QSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLT 561
PKPILLDTGEM PYDWAPSIL +Q+LRIGQL IL VPGEFTTMAGRRLR AVKT L
Sbjct: 499 HYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLK 558
Query: 562 TGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLA 621
++ +G +HVVIAGL N YSQYVTTFEEY++QRYEGASTLYGPHTL YIQEFKKL+
Sbjct: 559 NSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLS 618
Query: 622 QSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRGN-LV 681
+SL+ PV+PGPQPPDLL +Q+S LTPV+MD TP G SFGDV SDVP N + KRGN V
Sbjct: 619 KSLVLDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQV 678
Query: 682 TVTFWSGCPRNDLMTEGTFALVEILQKKD-TWVPAYDDDDFCLRFKWSRPSRLSPQSYAT 741
TV F S CPRNDL+TEGTF LVE L++KD TW P YDDDD CLRFKWSR +LS +S AT
Sbjct: 679 TVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQAT 738
Query: 742 IEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
+EWRIP++A GVYRI HFGAAK L GS+ HFTGSSSAFVV
Sbjct: 739 VEWRIPESASPGVYRITHFGAAKKLFGSVHHFTGSSSAFVV 778
BLAST of Clc03G01130 vs. ExPASy Swiss-Prot
Match:
Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)
HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 558/759 (73.52%), Postives = 652/759 (85.90%), Query Frame = 0
Query: 22 IYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAFI 81
I L+LL +R+V + YLIG+GS+DITGPAADVNMMGYAN++QIASG+HFRL ARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIHTHAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSFDV+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NPA+ERS+YKYDVDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQSRPGRPATR 321
FMEDWFE ++ + +IPRRVS I+ + +N+ LL+ AA+++S G +
Sbjct: 251 FMEDWFENGQKNSV------SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDK 310
Query: 322 IVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNE 381
+ V RVRN + +FVSAFCQSNCGDVSPNTLG FCIDTGLPCDFNHSTC G+NE
Sbjct: 311 SLDVKTRVRN----GSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNE 370
Query: 382 LCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTK 441
LCYGRGPGYPDEFEST IIGE+QF+ AV+LF+KA+E+L GK+ Y+H+Y+DFS L+V++ K
Sbjct: 371 LCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPK 430
Query: 442 QGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIAC 501
GG +E VKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+L+ PG EQ+ C
Sbjct: 431 AGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQC 490
Query: 502 QSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLT 561
Q PKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLRDA+K+ L
Sbjct: 491 QKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLI 550
Query: 562 TGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLA 621
+ +EF+ N+HVVIAGLTNTYSQY+ TFEEYE+QRYEGASTLYG HTL AYIQEFKKLA
Sbjct: 551 SSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLA 610
Query: 622 QSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRGNLVT 681
+L++G + GPQPPDLL +QISLL+PV++D+TP+GV FGDVK+DVP STF+RG V
Sbjct: 611 TALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVN 670
Query: 682 VTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIE 741
TFWSGCPRNDLMTEG+FA+VE L++ W P YDDDDF L+FKWSRP++LS +S ATIE
Sbjct: 671 ATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIE 730
Query: 742 WRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
WR+P++AV+GVYRIRH+GA+KSL GSI F+GSSSAFVV
Sbjct: 731 WRVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVV 756
BLAST of Clc03G01130 vs. ExPASy Swiss-Prot
Match:
Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)
HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 554/777 (71.30%), Postives = 648/777 (83.40%), Query Frame = 0
Query: 13 RSLGS--IWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASG 72
R GS +WL + + ++L + VLS S YL+G+GS DITGPAADVNMMGYAN EQIASG
Sbjct: 12 RGFGSSRVWLWLLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASG 71
Query: 73 VHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGI 132
+HFRL +RAFIVAEP GKRVVFVN+DACMASQIVTIKVLERLKARYGDLY E NVAISGI
Sbjct: 72 IHFRLKSRAFIVAEPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGI 131
Query: 133 HTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDA 192
HTHAGPGGYLQYVVYIVTSLGFVRQSFDV+VDGIE+SI++AH NL PG I +NKG+L+DA
Sbjct: 132 HTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDA 191
Query: 193 GVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSL 252
GVNRSPSAYLNNPA ERS+Y+Y+VDKEMTL+KFVDDE GPVGSFNWFATHGTSMSRTNSL
Sbjct: 192 GVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELGPVGSFNWFATHGTSMSRTNSL 251
Query: 253 ISGDNKGAAARFMEDWFEQKGV-RTLLHPGESEADS------IPRRVSNIIPEVYKNKQE 312
ISGDNKGAAARFMEDW EQ G+ + H + S +PRRVS IIPE + +
Sbjct: 252 ISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDD 311
Query: 313 LLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCI 372
L++ A+S+++ GR S++RR+R+ Q ++P+FVSAFCQSNCGDVSPN LG FCI
Sbjct: 312 LIQLASSYEASGGRRLAG-SSITRRIRST--QQNKPKFVSAFCQSNCGDVSPNVLGTFCI 371
Query: 373 DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKV 432
DT LPCDFNHSTC GKNELCYGRGPGYPDEFEST +IG RQF KA DLF ASE++ GK+
Sbjct: 372 DTNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKI 431
Query: 433 DYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAF 492
DYRH+Y+DFS+LEV ++ G + VKTCPAAMGFAFAAGTTDGPGAFDF+QGD KGN F
Sbjct: 432 DYRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPF 491
Query: 493 WKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGE 552
WKLVRNLLK PG +Q+ C SPKPILLDTGEMK PYDWAP+ILP+Q++RIGQLVIL VPGE
Sbjct: 492 WKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGE 551
Query: 553 FTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGAST 612
FTTMAGRRLRDAVKTVLT+G EF+ N+HVV+AGLTN+YSQY+TTFEEY++QRYEGAST
Sbjct: 552 FTTMAGRRLRDAVKTVLTSG-NSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGAST 611
Query: 613 LYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGD 672
LYGPHTL AYIQEF+KLA ++I + V QPPD+L +QI LL V+ D+TP+GV FGD
Sbjct: 612 LYGPHTLSAYIQEFQKLAMAMIANKEVPTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGD 671
Query: 673 VKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLR 732
V SDVP NSTF +G+ V TF+S CPRNDL+T+GTFALVE L + WVP YDDDD+ LR
Sbjct: 672 VNSDVPGNSTFNKGSTVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLR 731
Query: 733 FKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
FKWSRP+RLS +S+AT+EW +P+ A +GVYR+RHFGA+K + GS+RHFTG+S AF V
Sbjct: 732 FKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 784
BLAST of Clc03G01130 vs. ExPASy Swiss-Prot
Match:
F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)
HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 546/764 (71.47%), Postives = 622/764 (81.41%), Query Frame = 0
Query: 21 CIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAF 80
C L+ L + S+S YL+GLGS+DITGPAADVNMMGYAN EQ+ASGVHFRL ARAF
Sbjct: 10 CTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAF 69
Query: 81 IVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYL 140
IVAEP KR+ FVNLDA MASQ+VTIKV+ERLK RYG+LYTE+NVAISG HTHAGPGGYL
Sbjct: 70 IVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYL 129
Query: 141 QYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYL 200
QY++Y+VTSLGFV QSF+ LVDGIE+SIIQAHENL PGSILINKGEL+DAGVNRSPSAYL
Sbjct: 130 QYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL 189
Query: 201 NNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAA 260
NNPA ERS+Y+YDVDKEMTL+KFVDD+WGPV A
Sbjct: 190 NNPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------A 249
Query: 261 RFMEDWFE-QKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQSRPGRPA 320
R MEDWFE + G R++ + +S PRRVS+II + Y Q+L+E A+S S G+
Sbjct: 250 RIMEDWFERENGCRSV------DVES-PRRVSSIISDPY--DQDLMEMASSLLSTGGKTV 309
Query: 321 TRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGK 380
TR+ SV+RRVR+ R AD+P+FVSAFCQ+NCGDVSPN LGAFCIDTGLPC+FN STCGGK
Sbjct: 310 TRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGK 369
Query: 381 NELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSL 440
NE CYGRGPGYPDEFEST IIGERQF+KA DLF+KASE++ GKVDYRH+YVDFSQLEV++
Sbjct: 370 NEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTI 429
Query: 441 TKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQI 500
Q G +EVVKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRNLLK P EQ+
Sbjct: 430 NGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQV 489
Query: 501 ACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTV 560
CQ PKPILLDTGEMK PYDWAPSILP+QILRIGQLVIL VPGEFTTMAGRRLRDAVKTV
Sbjct: 490 RCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTV 549
Query: 561 LTTGAK-REFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFK 620
L G+ REF+ VVIAGLTN+YSQY+ TFEEY++QRYEGASTLYGPHTL YIQEFK
Sbjct: 550 LKEGSNGREFS----VVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFK 609
Query: 621 KLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRG- 680
KLA L+ + +PGPQPPDLL +QISLLTPV+ D TPIG +FGDV SDVP S F++G
Sbjct: 610 KLANDLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGA 669
Query: 681 NLVTVTFWSGCPRNDLMTEGTFALVE-ILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQS 740
++V V F S PRNDLMTEGTFALVE L+ ++TWVP YDDDDFCLRFKWSRP +LS QS
Sbjct: 670 DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQS 729
Query: 741 YATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
ATIEWRIP+TA GVYRI HFG+AK+ + SI HF+GSSSAFVV
Sbjct: 730 TATIEWRIPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVV 732
BLAST of Clc03G01130 vs. ExPASy Swiss-Prot
Match:
Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)
HSP 1 Score: 602.8 bits (1553), Expect = 5.4e-171
Identity = 335/773 (43.34%), Postives = 450/773 (58.21%), Query Frame = 0
Query: 14 SLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHF 73
S +I++ + + + Q N +S Y IG G +DITG +A+VN+MGYAN Q+ +G+HF
Sbjct: 20 SSNNIFIDSFKIPVNQKNVKSSGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHF 79
Query: 74 RLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYG-DLYTEKNVAISGIHT 133
R ARAF+ + G R V+V+ D+CM Q V I V+E L+ +G ++YTE NV +SG HT
Sbjct: 80 RQRARAFVFVDSNGNRAVYVSTDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHT 139
Query: 134 HAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGV 193
H+GP G+ QY +Y +TSLGF +++FD + +GI ++I++AH+++ P ++ GEL + +
Sbjct: 140 HSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNI 199
Query: 194 NRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLIS 253
NRSP AY NNP E++ Y +VDK MT+L+ D P + ++FA H TSM+ TN LIS
Sbjct: 200 NRSPFAYDNNPEEEKAMYDSNVDKNMTVLRIEDMNGNPFAAISFFAVHCTSMNNTNHLIS 259
Query: 254 GDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQ 313
GDNKG A+ E G
Sbjct: 260 GDNKGYASYLWEKQVNGPGT---------------------------------------- 319
Query: 314 SRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFN 373
A + FV+AF QSN GDVSPNT G C D G PCD+
Sbjct: 320 -----------------------AGKGPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYK 379
Query: 374 HSTCGGKNELCYGRGPGYP-DEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVD 433
STC G+NE C+ GPG D FEST IIG QF KA++LF+ AS Q++G V YRHS+V
Sbjct: 380 TSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQ 439
Query: 434 FSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDD--KGNAFWKLVRN 493
F+ + V G TC AMG++FAAGTTDGPGAF+F Q D+ GN FW + +
Sbjct: 440 FTNVSVEPPYNSGVDNAT-TCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGD 499
Query: 494 LLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAG 553
+ P +QI CQSPKPILLD G M P W P ++PIQI+ IGQ+V++ VPGEFTTM+G
Sbjct: 500 FIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSG 559
Query: 554 RRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHT 613
RRLR++V+ ++ + N V+IAGL+NTYS Y+ TFEEY++QRYEGAST++GPHT
Sbjct: 560 RRLRNSVREIIGESIE-----NPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHT 619
Query: 614 LDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVP 673
L +Y+QEF KLAQS++DG V GP P +L + L PVI+DA P FG+V DV
Sbjct: 620 LGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVN 679
Query: 674 LNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLRFKWSRP 733
LN + V+ F+ G PRND M E +F V++L D W DD D+ +FKW
Sbjct: 680 LN--YSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMH 717
Query: 734 SRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKS--LLGSIRHFTGSSSAFVV 781
S TIEW I G YRI H G AK ++ + G S FVV
Sbjct: 740 D--LGFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717
BLAST of Clc03G01130 vs. ExPASy TrEMBL
Match:
A0A6J1HIT9 (Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1)
HSP 1 Score: 1491.5 bits (3860), Expect = 0.0e+00
Identity = 736/781 (94.24%), Postives = 758/781 (97.06%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD RRSLGSIWLC+Y+LV+LQSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RYKYDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKG+ T L G+SEADSIPRRVSNIIPE Y +
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGT-LQTGKSEADSIPRRVSNIIPEAYND 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
+QELL RAASFQSRPGRPATRI+SVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 EQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQL
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLK 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
G VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV+MDATP GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDV+SDVPLNSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDF
Sbjct: 661 FGDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. ExPASy TrEMBL
Match:
A0A6J1I331 (Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111469470 PE=3 SV=1)
HSP 1 Score: 1486.5 bits (3847), Expect = 0.0e+00
Identity = 735/781 (94.11%), Postives = 755/781 (96.67%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD RRSLGSIWLC+Y+LV+LQSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQGK+VVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKQVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDA VNRSPSAYLNNPASER RYKYDVDKEMTLLKFVDD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDASVNRSPSAYLNNPASERDRYKYDVDKEMTLLKFVDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKG+RT L G+SEADSIPRRVSNIIPE YK+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIRT-LQTGKSEADSIPRRVSNIIPEAYKD 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
+QELLERAA+FQSRPGRPATRI+SVSRRVRN LRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 EQELLERAAAFQSRPGRPATRILSVSRRVRNALRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLFSKASEQL
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLK 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
G VDYRHSYVDFSQLEVS+TK GGAT VVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GTVDYRHSYVDFSQLEVSVTKPGGATAVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRNLLK PG+EQIACQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NAFWKLVRNLLKTPGSEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV+MDATP GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVPLNSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDF
Sbjct: 661 FGDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKW RPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK+LL SIRHFTGSSSAFVV
Sbjct: 721 CLRFKWWRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKNLLRSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. ExPASy TrEMBL
Match:
A0A6J1DAR9 (Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1)
HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 726/781 (92.96%), Postives = 748/781 (95.77%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSL DL RSL ++WL I +LVLLQSN VLSESKYLIGLGS+DITGPAADVNMMG
Sbjct: 1 MGLSSLVDLTVGRSLATLWLWICMLVLLQSNGRVLSESKYLIGLGSYDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASGVHFRL ARAFIVAEPQG RVVFVNLDACMASQ+VTIKVLERLKARYGDLY
Sbjct: 61 YANIEQIASGVHFRLRARAFIVAEPQGNRVVFVNLDACMASQLVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGS+
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSV 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LIN GELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH
Sbjct: 181 LINTGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWF+QKG+ + LHPG+S ADSIPRRVSNIIPE YKN
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFDQKGIGS-LHPGQSVADSIPRRVSNIIPEAYKN 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
KQELLERAASFQSRPGRPATRI+SV+RRVRNVLRQADRPQFVSAFCQSNCGDVSPN LGA
Sbjct: 301 KQELLERAASFQSRPGRPATRILSVARRVRNVLRQADRPQFVSAFCQSNCGDVSPNVLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFES IIGERQFRKAVDLF+KASE+LT
Sbjct: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESARIIGERQFRKAVDLFNKASEKLT 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
GKVDYRHSYVDFSQLEVSL KQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GKVDYRHSYVDFSQLEVSLLKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
N FWKLVRNLL+APG +Q+ CQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV
Sbjct: 481 NPFWKLVRNLLRAPGEQQVTCQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLR+AVKTVLTTGAKREFNGNVH+VIAGLTNTYSQYVTTFEEYEMQRYEG
Sbjct: 541 PGEFTTMAGRRLRNAVKTVLTTGAKREFNGNVHIVIAGLTNTYSQYVTTFEEYEMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLL+RQISLL PVIMDATP+GVS
Sbjct: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLSRQISLLPPVIMDATPLGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP YDDDDF
Sbjct: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPVYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CL FKW RP R S QSYATIEWRIPQ+AVSGVYRIRHFGAAKSL GSIRHFTGSSSAFVV
Sbjct: 721 CLFFKWLRPFRYSTQSYATIEWRIPQSAVSGVYRIRHFGAAKSLFGSIRHFTGSSSAFVV 780
Query: 781 A 782
A
Sbjct: 781 A 780
BLAST of Clc03G01130 vs. ExPASy TrEMBL
Match:
A0A0A0LY63 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1)
HSP 1 Score: 1458.0 bits (3773), Expect = 0.0e+00
Identity = 719/781 (92.06%), Postives = 752/781 (96.29%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLF+L RSLG+IWLCIY+LVL +SNRSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSESNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANA+QIASG+HFRL ARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERS+YKYDVDKEMTLLKF+DDEWGPVG+FNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTN+LISGDNKGAAARFMEDWF+QKG T LH GESEADSIPRRVSNI+PEVYK+
Sbjct: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGT-LHHGESEADSIPRRVSNIVPEVYKD 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
KQELLE AASFQS+PGRPATR++S+S RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 KQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGE+QFRKAVDLFSKASEQLT
Sbjct: 361 FCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLT 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
GKVD+RHSYVDFS LEVSL KQGG TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
NAFWKLVRN+LKAPGNEQI+CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL V
Sbjct: 481 NAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILDV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPDLLARQISLL PVI+D TP+GVS
Sbjct: 601 ASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVK DVP NS+FKRGNLV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVPAYDDDDF
Sbjct: 661 FGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRP+ LS QSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
Query: 781 A 782
A
Sbjct: 781 A 779
BLAST of Clc03G01130 vs. ExPASy TrEMBL
Match:
A0A1S3C5F7 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)
HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 721/780 (92.44%), Postives = 744/780 (95.38%), Query Frame = 0
Query: 1 MELSSLFDLIGRRSLGSIWLCIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLF+L RRSLG+IWLCIY+LVL QS RSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVRRSLGTIWLCIYMLVLSQSTRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGVHFRLLARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANAEQIASGVHFRL ARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIH+HAGPGGYLQY+VYIVTSLGFVRQSFDVLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASE S+YKYDVDKEMTLLKF+DDEWGPVG+FNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKN 300
GTSMSRTNSLISGDNKGAAARFMEDWFEQKG T LHPGESEADSIPRRVSNIIPEVYK+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGT-LHPGESEADSIPRRVSNIIPEVYKD 300
Query: 301 KQELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
KQELLERAASFQS+PGRPATR +SVS RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA
Sbjct: 301 KQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGA 360
Query: 361 FCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLT 420
FC+DT LPCDFNHSTCGGKNELCYGRGPGYPDEFEST IIGERQFRKAVDLF KASE LT
Sbjct: 361 FCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFGKASEHLT 420
Query: 421 GKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
GKVD+RHSYVDFS LEVSLTKQGGA EVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG
Sbjct: 421 GKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKG 480
Query: 481 NAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGV 540
N FWKLVRNLLKAPGNEQI+CQSPKPILLDTGEMK PYDWAP+ILPIQILRIGQLVIL V
Sbjct: 481 NPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRIGQLVILSV 540
Query: 541 PGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEG 600
PGEFTTMAGRRLRDAVKTVLTTGA +EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEG
Sbjct: 541 PGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEG 600
Query: 601 ASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVS 660
ASTLYGPHTL+AYIQEFKKLAQSLIDGRPV PGPQPPDLL RQISLL PVI+D TP GVS
Sbjct: 601 ASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVILDMTPPGVS 660
Query: 661 FGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDF 720
FGDVKSDVP NS+FKRGNLV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVPAYDDDDF
Sbjct: 661 FGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDF 720
Query: 721 CLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 780
CLRFKWSRP+ LS QSYATIEWRIPQT VSGVYRIRHFGAAKS GSIRHFTGSSSAFVV
Sbjct: 721 CLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTGSSSAFVV 778
BLAST of Clc03G01130 vs. TAIR 10
Match:
AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 576/761 (75.69%), Postives = 647/761 (85.02%), Query Frame = 0
Query: 22 IYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAFI 81
I ++ L + V S S+YLIGLGS+DITGPAADVNMMGYAN EQ+ASG+HFRL AR FI
Sbjct: 19 ISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFI 78
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
V+EPQGKRVVFVNLDACMASQIV +KV+ERLKARYGDLYTE+NV ISGIHTHAGPGGYLQ
Sbjct: 79 VSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQ 138
Query: 142 YVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YVVYIVTSLGFVRQSFD LVDGIE SIIQAHENL PGSI +N GEL+DAGVNRSPSAYLN
Sbjct: 139 YVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLN 198
Query: 202 NPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NP+ ERS++KY+VDKEMTLLKFVDD+WGPVGSFNWFATHGTSMSRTNSLISGDNKGAA+R
Sbjct: 199 NPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASR 258
Query: 262 FMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQSRPGRPATR 321
FMEDW+EQ + E +D IPRRVS++I + ELLE A+ F+S+PG+P TR
Sbjct: 259 FMEDWYEQNTAER-SYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTR 318
Query: 322 IVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNE 381
I S +RRVR+ LR+AD+P FVSAFCQ+NCGDVSPN LGAFC+DTGLPCDFNHSTCGGKNE
Sbjct: 319 ISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNE 378
Query: 382 LCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTK 441
+CYGRGPGYPDEFEST IIGERQF+ A++LF+KASEQL GKVDYRH YVDFSQL V+L K
Sbjct: 379 MCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPK 438
Query: 442 QGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIAC 501
+ G +EVVKTCPAAMGFAFAAGTTDGPGAFDF QGDDKGN FW+LVRN+LK P +QI C
Sbjct: 439 KDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDC 498
Query: 502 QSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLT 561
PKPILLDTGEM PYDWAPSIL +Q+LRIGQL IL VPGEFTTMAGRRLR AVKT L
Sbjct: 499 HYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLK 558
Query: 562 TGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLA 621
++ +G +HVVIAGL N YSQYVTTFEEY++QRYEGASTLYGPHTL YIQEFKKL+
Sbjct: 559 NSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLS 618
Query: 622 QSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRGN-LV 681
+SL+ PV+PGPQPPDLL +Q+S LTPV+MD TP G SFGDV SDVP N + KRGN V
Sbjct: 619 KSLVLDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQV 678
Query: 682 TVTFWSGCPRNDLMTEGTFALVEILQKKD-TWVPAYDDDDFCLRFKWSRPSRLSPQSYAT 741
TV F S CPRNDL+TEGTF LVE L++KD TW P YDDDD CLRFKWSR +LS +S AT
Sbjct: 679 TVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQAT 738
Query: 742 IEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
+EWRIP++A GVYRI HFGAAK L GS+ HFTGSSSAFVV
Sbjct: 739 VEWRIPESASPGVYRITHFGAAKKLFGSVHHFTGSSSAFVV 778
BLAST of Clc03G01130 vs. TAIR 10
Match:
AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 558/759 (73.52%), Postives = 652/759 (85.90%), Query Frame = 0
Query: 22 IYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAFI 81
I L+LL +R+V + YLIG+GS+DITGPAADVNMMGYAN++QIASG+HFRL ARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIHTHAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSFDV+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NPA+ERS+YKYDVDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQSRPGRPATR 321
FMEDWFE ++ + +IPRRVS I+ + +N+ LL+ AA+++S G +
Sbjct: 251 FMEDWFENGQKNSV------SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDK 310
Query: 322 IVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNE 381
+ V RVRN + +FVSAFCQSNCGDVSPNTLG FCIDTGLPCDFNHSTC G+NE
Sbjct: 311 SLDVKTRVRN----GSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNE 370
Query: 382 LCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTK 441
LCYGRGPGYPDEFEST IIGE+QF+ AV+LF+KA+E+L GK+ Y+H+Y+DFS L+V++ K
Sbjct: 371 LCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPK 430
Query: 442 QGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIAC 501
GG +E VKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+L+ PG EQ+ C
Sbjct: 431 AGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQC 490
Query: 502 QSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLT 561
Q PKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLRDA+K+ L
Sbjct: 491 QKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLI 550
Query: 562 TGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLA 621
+ +EF+ N+HVVIAGLTNTYSQY+ TFEEYE+QRYEGASTLYG HTL AYIQEFKKLA
Sbjct: 551 SSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLA 610
Query: 622 QSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRGNLVT 681
+L++G + GPQPPDLL +QISLL+PV++D+TP+GV FGDVK+DVP STF+RG V
Sbjct: 611 TALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVN 670
Query: 682 VTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIE 741
TFWSGCPRNDLMTEG+FA+VE L++ W P YDDDDF L+FKWSRP++LS +S ATIE
Sbjct: 671 ATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIE 730
Query: 742 WRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
WR+P++AV+GVYRIRH+GA+KSL GSI F+GSSSAFVV
Sbjct: 731 WRVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVV 756
BLAST of Clc03G01130 vs. TAIR 10
Match:
AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 558/794 (70.28%), Postives = 653/794 (82.24%), Query Frame = 0
Query: 22 IYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAFI 81
I L+LL +R+V + YLIG+GS+DITGPAADVNMMGYAN++QIASG+HFRL ARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIHTHAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSFDV+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NPA+ERS+YKYDVDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFEQKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQ------------------- 321
FMEDWFE ++ + +IPRRVS I+ + +N++
Sbjct: 251 FMEDWFENGQKNSV------SSRNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAE 310
Query: 322 ----------------ELLERAASFQSRPGRPATRIVSVSRRVRNVLRQADRPQFVSAFC 381
LL+ AA+++S G + + V RVRN + +FVSAFC
Sbjct: 311 RLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRVRN----GSKRKFVSAFC 370
Query: 382 QSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTCIIGERQFR 441
QSNCGDVSPNTLG FCIDTGLPCDFNHSTC G+NELCYGRGPGYPDEFEST IIGE+QF+
Sbjct: 371 QSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFK 430
Query: 442 KAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTD 501
AV+LF+KA+E+L GK+ Y+H+Y+DFS L+V++ K GG +E VKTCPAAMGF FAAGTTD
Sbjct: 431 MAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTD 490
Query: 502 GPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKAPYDWAPSILP 561
GPGAFDFKQGDD+GN FW+LVRN+L+ PG EQ+ CQ PKPILLDTGEMK PYDWAPSILP
Sbjct: 491 GPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILP 550
Query: 562 IQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQY 621
IQILRIGQLVIL VPGEFTTMAGRRLRDA+K+ L + +EF+ N+HVVIAGLTNTYSQY
Sbjct: 551 IQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQY 610
Query: 622 VTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISL 681
+ TFEEYE+QRYEGASTLYG HTL AYIQEFKKLA +L++G + GPQPPDLL +QISL
Sbjct: 611 IATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDLLDKQISL 670
Query: 682 LTPVIMDATPIGVSFGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQ 741
L+PV++D+TP+GV FGDVK+DVP STF+RG V TFWSGCPRNDLMTEG+FA+VE L+
Sbjct: 671 LSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLR 730
Query: 742 KKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLG 781
+ W P YDDDDF L+FKWSRP++LS +S ATIEWR+P++AV+GVYRIRH+GA+KSL G
Sbjct: 731 EGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFG 790
BLAST of Clc03G01130 vs. TAIR 10
Match:
AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 546/764 (71.47%), Postives = 622/764 (81.41%), Query Frame = 0
Query: 21 CIYILVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGVHFRLLARAF 80
C L+ L + S+S YL+GLGS+DITGPAADVNMMGYAN EQ+ASGVHFRL ARAF
Sbjct: 10 CTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAF 69
Query: 81 IVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYL 140
IVAEP KR+ FVNLDA MASQ+VTIKV+ERLK RYG+LYTE+NVAISG HTHAGPGGYL
Sbjct: 70 IVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYL 129
Query: 141 QYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYL 200
QY++Y+VTSLGFV QSF+ LVDGIE+SIIQAHENL PGSILINKGEL+DAGVNRSPSAYL
Sbjct: 130 QYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL 189
Query: 201 NNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAA 260
NNPA ERS+Y+YDVDKEMTL+KFVDD+WGPV A
Sbjct: 190 NNPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------A 249
Query: 261 RFMEDWFE-QKGVRTLLHPGESEADSIPRRVSNIIPEVYKNKQELLERAASFQSRPGRPA 320
R MEDWFE + G R++ + +S PRRVS+II + Y Q+L+E A+S S G+
Sbjct: 250 RIMEDWFERENGCRSV------DVES-PRRVSSIISDPY--DQDLMEMASSLLSTGGKTV 309
Query: 321 TRIVSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGK 380
TR+ SV+RRVR+ R AD+P+FVSAFCQ+NCGDVSPN LGAFCIDTGLPC+FN STCGGK
Sbjct: 310 TRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGK 369
Query: 381 NELCYGRGPGYPDEFESTCIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSL 440
NE CYGRGPGYPDEFEST IIGERQF+KA DLF+KASE++ GKVDYRH+YVDFSQLEV++
Sbjct: 370 NEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTI 429
Query: 441 TKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQI 500
Q G +EVVKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRNLLK P EQ+
Sbjct: 430 NGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQV 489
Query: 501 ACQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTV 560
CQ PKPILLDTGEMK PYDWAPSILP+QILRIGQLVIL VPGEFTTMAGRRLRDAVKTV
Sbjct: 490 RCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTV 549
Query: 561 LTTGAK-REFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFK 620
L G+ REF+ VVIAGLTN+YSQY+ TFEEY++QRYEGASTLYGPHTL YIQEFK
Sbjct: 550 LKEGSNGREFS----VVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFK 609
Query: 621 KLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRG- 680
KLA L+ + +PGPQPPDLL +QISLLTPV+ D TPIG +FGDV SDVP S F++G
Sbjct: 610 KLANDLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGA 669
Query: 681 NLVTVTFWSGCPRNDLMTEGTFALVE-ILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQS 740
++V V F S PRNDLMTEGTFALVE L+ ++TWVP YDDDDFCLRFKWSRP +LS QS
Sbjct: 670 DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQS 729
Query: 741 YATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 781
ATIEWRIP+TA GVYRI HFG+AK+ + SI HF+GSSSAFVV
Sbjct: 730 TATIEWRIPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVV 732
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4HQM3 | 0.0e+00 | 75.69 | Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1 | [more] |
| Q304B9 | 0.0e+00 | 73.52 | Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1 | [more] |
| Q0JL46 | 0.0e+00 | 71.30 | Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1... | [more] |
| F4KHQ8 | 0.0e+00 | 71.47 | Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1 | [more] |
| Q55G11 | 5.4e-171 | 43.34 | Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1HIT9 | 0.0e+00 | 94.24 | Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1 | [more] |
| A0A6J1I331 | 0.0e+00 | 94.11 | Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111469470 PE=3 SV=1 | [more] |
| A0A6J1DAR9 | 0.0e+00 | 92.96 | Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1 | [more] |
| A0A0A0LY63 | 0.0e+00 | 92.06 | Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1 | [more] |
| A0A1S3C5F7 | 0.0e+00 | 92.44 | Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1 | [more] |
