Clc02G00710 (gene) Watermelon (cordophanus) v2

Overview
NameClc02G00710
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionRibosome assembly factor mrt4
LocationClcChr02: 640106 .. 641627 (-)
RNA-Seq ExpressionClc02G00710
SyntenyClc02G00710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTAAGTCGAAGCGTGATAGACCAGGTCCGTTTTGATCTATTTTTTGCTCATATCTGTGAATCTGTCGCGTTATTTTGTGTTTTTGTTGGTCTGTTCTATCTGCCTTGTTTTCCATGCTAAAAGAAATAGATTTTAGCTCATCTGGTTATTGTGCTGTAATATGAAAATCTGTTACAGGAACCTCACACAGTACAAGGAGGGATTGGTTAAGCTATATTACGGGAAATAGCCACTAGAAAAGTTGTAGTTGTAAGATAGAAGTTCTTTTTTTTTTTTTTTTTTTTTTGTTGGGTGTATTCTTGCCTCTTATGAGGGGAACTTCATTCTGAAGTGCTTAGTTCACTAGGTTGGCTGCAAGTCGACCAGCATTTGTGTTGTTACATTGCATCATGTTATAGGACTTGAATGTAAAGATATATGAGGCTCTTTAATATGCACACAAAACCAGTTCAGCACCATGACACTGTCAAGTGCCTTATATTTAGTTCAGTATACCTTTTCTTATAGCTATTTGAGCTGCATATTAAAGAAATCAAAATAGGATAGATTCCATAAATATTAGCATCTTTCTTGCTCGATTTGAGCTGCATATTATTCTTCTAAGGCTCTTCAAAGTTCTATTGAAGTTATTTGATGTGTTACTTGAGCAAGTACTTTAAGAGTCAGGAATTTGTAGAGGCTTCCTAATAGGAGTAGGTCATTTGCTACCCACTCCTTGAACTAAGGGTTATTACAAATCTGATGCTCTGTGAGCCTCAAGCTTGCTTTCCTTGATGGTAGGCATGGCTTTAGTGTGCCCTAGAAGATAGTTTGTCATCAAGTTTGAGGGAAAAATCAATTCAGTATAGGTGTTGCCAGGAGGTGGCTGAGGTGAAAGTTTCTGTGTATGTACAAAATGTAGAGTTGCTTACAACATATGGAACATGATGTCTAAAGTTACAGCGTTTACTGCGTGAGACTAATTTCTAGAAAAATAAATTTAGTAGGCTGATTCATGTTGTTAAACAGAATCCAAATAACAGTAACCTTAATAGAATTTAATGACTTGCTTTAAGAGTAGAAAGATGCTAAAATTTGTTCTTTTAGTTCAAGGTTCTTGTTGAATATGGGTTAACGGGTTAGTTATATTATGGGTTAATTTTTTTGAGGCAGTTTAAGAAGTTAAATGACTTGTGGTGGTAGTATTCAGTGTCAAACTTGTGTGGTTTTTAACTTAAAAGAAAGAGGAAAAATATGAACTTTTAGTCCATTGCAGATTAGTGTCTTGACTAATTGTTCTGTGGGATAAATGCAGTTACTTTATCAAAAACCAAGAAGAAAGGAAGGGAACACAAGAAATCAATGGTGGATCTGATCAGGCAGGCTGTGGAAAATTACAACTCAATTTTTGTTTTCACTGTTGAGAACATGAGAAACCTCAAGTTTAAGGAACTCAGGGAGCATCTGAAGTCATCTAGCAGGCAAGTTGTATTTCCTGACTTCTGTATTATTTATTTGCTACATCATTTCAAATATTGA

mRNA sequence

ATGCCTAAGTCGAAGCGTGATAGACCAGGTCCGAACCTCACACAGTACAAGGAGGGATTGTTCAAGGTTCTTGTTGAATATGGGTTAACGGTTACTTTATCAAAAACCAAGAAGAAAGGAAGGGAACACAAGAAATCAATGGTGGATCTGATCAGGCAGGCTGTGGAAAATTACAACTCAATTTTTGTTTTCACTGTTGAGAACATGAGAAACCTCAAGTTTAAGGAACTCAGGGAGCATCTGAAGTCATCTAGCAGGCAAGTTGTATTTCCTGACTTCTGTATTATTTATTTGCTACATCATTTCAAATATTGA

Coding sequence (CDS)

ATGCCTAAGTCGAAGCGTGATAGACCAGGTCCGAACCTCACACAGTACAAGGAGGGATTGTTCAAGGTTCTTGTTGAATATGGGTTAACGGTTACTTTATCAAAAACCAAGAAGAAAGGAAGGGAACACAAGAAATCAATGGTGGATCTGATCAGGCAGGCTGTGGAAAATTACAACTCAATTTTTGTTTTCACTGTTGAGAACATGAGAAACCTCAAGTTTAAGGAACTCAGGGAGCATCTGAAGTCATCTAGCAGGCAAGTTGTATTTCCTGACTTCTGTATTATTTATTTGCTACATCATTTCAAATATTGA

Protein sequence

MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNSIFVFTVENMRNLKFKELREHLKSSSRQVVFPDFCIIYLLHHFKY
Homology
BLAST of Clc02G00710 vs. NCBI nr
Match: XP_022933518.1 (mRNA turnover protein 4 homolog [Cucurbita moschata] >KAG6588508.1 mRNA turnover protein 4-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.5 bits (280), Expect = 2.3e-21
Identity = 63/86 (73.26%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKSSSR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSSSR 65

BLAST of Clc02G00710 vs. NCBI nr
Match: XP_022969955.1 (mRNA turnover protein 4 homolog [Cucurbita maxima])

HSP 1 Score: 112.5 bits (280), Expect = 2.3e-21
Identity = 63/86 (73.26%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKSSSR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSSSR 65

BLAST of Clc02G00710 vs. NCBI nr
Match: XP_023529350.1 (mRNA turnover protein 4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 112.5 bits (280), Expect = 2.3e-21
Identity = 63/86 (73.26%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKSSSR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSSSR 65

BLAST of Clc02G00710 vs. NCBI nr
Match: XP_038902849.1 (mRNA turnover protein 4 homolog [Benincasa hispida])

HSP 1 Score: 112.1 bits (279), Expect = 2.9e-21
Identity = 63/86 (73.26%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMV+LIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVELIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          IFVFTVENMRNLKFKELRE LKSSSR
Sbjct: 61 IFVFTVENMRNLKFKELREQLKSSSR 65

BLAST of Clc02G00710 vs. NCBI nr
Match: XP_022157470.1 (mRNA turnover protein 4 homolog [Momordica charantia])

HSP 1 Score: 111.3 bits (277), Expect = 5.0e-21
Identity = 62/86 (72.09%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKS+SR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSTSR 65

BLAST of Clc02G00710 vs. ExPASy Swiss-Prot
Match: A4FV84 (mRNA turnover protein 4 homolog OS=Bos taurus OX=9913 GN=MRTO4 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 5.6e-07
Identity = 31/84 (36.90%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRD+                      V+L+KT KKG E K+++++ +R+ V+ Y  
Sbjct: 1  MPKSKRDK---------------------KVSLTKTAKKGLELKQNLIEELRKCVDTYKY 60

Query: 61 IFVFTVENMRNLKFKELREHLKSS 85
          +F+F+V NMRN K K++R   K S
Sbjct: 61 LFIFSVANMRNSKLKDIRNAWKHS 63

BLAST of Clc02G00710 vs. ExPASy Swiss-Prot
Match: Q9UKD2 (mRNA turnover protein 4 homolog OS=Homo sapiens OX=9606 GN=MRTO4 PE=1 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 5.6e-07
Identity = 31/84 (36.90%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRD+                      V+L+KT KKG E K+++++ +R+ V+ Y  
Sbjct: 1  MPKSKRDK---------------------KVSLTKTAKKGLELKQNLIEELRKCVDTYKY 60

Query: 61 IFVFTVENMRNLKFKELREHLKSS 85
          +F+F+V NMRN K K++R   K S
Sbjct: 61 LFIFSVANMRNSKLKDIRNAWKHS 63

BLAST of Clc02G00710 vs. ExPASy Swiss-Prot
Match: Q9D0I8 (mRNA turnover protein 4 homolog OS=Mus musculus OX=10090 GN=Mrto4 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 5.6e-07
Identity = 31/84 (36.90%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRD+                      V+L+KT KKG E K+++++ +R+ V+ Y  
Sbjct: 1  MPKSKRDK---------------------KVSLTKTAKKGLELKQNLIEELRKCVDTYKY 60

Query: 61 IFVFTVENMRNLKFKELREHLKSS 85
          +F+F+V NMRN K K++R   K S
Sbjct: 61 LFIFSVANMRNSKLKDIRNAWKHS 63

BLAST of Clc02G00710 vs. ExPASy Swiss-Prot
Match: Q6FJ04 (Ribosome assembly factor mrt4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MRT4 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 3.4e-04
Identity = 20/49 (40.82%), Postives = 36/49 (73.47%), Query Frame = 0

Query: 31 VTLSKTKKKGREHKKSMVDLIRQAVENYNSIFVFTVENMRNLKFKELRE 80
          VTL++T KKGRE+K+ + D +R+A++ Y  +FV  ++++R    +E+RE
Sbjct: 10 VTLAQTDKKGRENKERIFDEVREALDTYKYVFVLQLDDVRTPVLQEIRE 58

BLAST of Clc02G00710 vs. ExPASy Swiss-Prot
Match: Q7S302 (Ribosome assembly factor mrt4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=mrt4 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 3.4e-04
Identity = 20/49 (40.82%), Postives = 33/49 (67.35%), Query Frame = 0

Query: 33 LSKTKKKGREHKKSMVDLIRQAVENYNSIFVFTVENMRNLKFKELREHL 82
          L++  KK RE K+ + + IR+ + NY   FVF+++NMRN   K++R+ L
Sbjct: 12 LTQVTKKNREQKEKLFENIRECIPNYQHCFVFSIDNMRNNYLKDVRKEL 60

BLAST of Clc02G00710 vs. ExPASy TrEMBL
Match: A0A6J1I1E2 (Ribosome assembly factor mrt4 OS=Cucurbita maxima OX=3661 GN=LOC111468997 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-21
Identity = 63/86 (73.26%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKSSSR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSSSR 65

BLAST of Clc02G00710 vs. ExPASy TrEMBL
Match: A0A6J1F4Z2 (Ribosome assembly factor mrt4 OS=Cucurbita moschata OX=3662 GN=LOC111440919 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-21
Identity = 63/86 (73.26%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKSSSR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSSSR 65

BLAST of Clc02G00710 vs. ExPASy TrEMBL
Match: A0A6J1DT71 (Ribosome assembly factor mrt4 OS=Momordica charantia OX=3673 GN=LOC111024162 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.4e-21
Identity = 62/86 (72.09%), Postives = 64/86 (74.42%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHKKSMVDLIRQAVENYNS
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKKSMVDLIRQAVENYNS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          I+VFTVENMRNLKFKELRE LKS+SR
Sbjct: 61 IYVFTVENMRNLKFKELREQLKSTSR 65

BLAST of Clc02G00710 vs. ExPASy TrEMBL
Match: A0A0A0KNA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G453720 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.9e-18
Identity = 48/62 (77.42%), Postives = 56/62 (90.32%), Query Frame = 0

Query: 35 KTKKKGREHKKSMVDLIRQAVENYNSIFVFTVENMRNLKFKELREHLKSSSRQVVFPDFC 94
          K ++KGR+HKKSMV+ IRQ VENYNS+FVFTVENMRNLKFKELRE LKS+SRQV+  +FC
Sbjct: 13 KQRRKGRDHKKSMVESIRQGVENYNSVFVFTVENMRNLKFKELREQLKSASRQVLLSEFC 72

Query: 95 II 97
          II
Sbjct: 73 II 74

BLAST of Clc02G00710 vs. ExPASy TrEMBL
Match: A0A4S4DS10 (Ribosome assembly factor mrt4 OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_022600 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-16
Identity = 51/92 (55.43%), Postives = 64/92 (69.57%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLV------EYGLTVTLSKTKKKGREHKKSMVDLIRQA 60
          MPKSKRDRPG + +         L       + G  +TLSKTKKKGR HK+S+V+ IR+ 
Sbjct: 1  MPKSKRDRPGLSSSPRSFNFIHCLAAEKPDGKRGSNLTLSKTKKKGRGHKESIVNSIREE 60

Query: 61 VENYNSIFVFTVENMRNLKFKELREHLKSSSR 87
          +E YNS++VF+ ENMRNLKFKE R+ LKSSSR
Sbjct: 61 IEKYNSVYVFSFENMRNLKFKEFRDKLKSSSR 92

BLAST of Clc02G00710 vs. TAIR 10
Match: AT1G25260.1 (Ribosomal protein L10 family protein )

HSP 1 Score: 81.3 bits (199), Expect = 5.2e-16
Identity = 43/86 (50.00%), Postives = 56/86 (65.12%), Query Frame = 0

Query: 1  MPKSKRDRPGPNLTQYKEGLFKVLVEYGLTVTLSKTKKKGREHKKSMVDLIRQAVENYNS 60
          MPKSKRDRP                     VTLSKTKKKGREHK+ +V+ IR+AVE Y+S
Sbjct: 1  MPKSKRDRP---------------------VTLSKTKKKGREHKECIVNGIREAVEKYSS 60

Query: 61 IFVFTVENMRNLKFKELREHLKSSSR 87
          ++VF+ ENMRN+KFKE R+  + + +
Sbjct: 61 VYVFSFENMRNIKFKEFRQQFRHNGK 65

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022933518.12.3e-2173.26mRNA turnover protein 4 homolog [Cucurbita moschata] >KAG6588508.1 mRNA turnover... [more]
XP_022969955.12.3e-2173.26mRNA turnover protein 4 homolog [Cucurbita maxima][more]
XP_023529350.12.3e-2173.26mRNA turnover protein 4 homolog [Cucurbita pepo subsp. pepo][more]
XP_038902849.12.9e-2173.26mRNA turnover protein 4 homolog [Benincasa hispida][more]
XP_022157470.15.0e-2172.09mRNA turnover protein 4 homolog [Momordica charantia][more]
Match NameE-valueIdentityDescription
A4FV845.6e-0736.90mRNA turnover protein 4 homolog OS=Bos taurus OX=9913 GN=MRTO4 PE=2 SV=1[more]
Q9UKD25.6e-0736.90mRNA turnover protein 4 homolog OS=Homo sapiens OX=9606 GN=MRTO4 PE=1 SV=2[more]
Q9D0I85.6e-0736.90mRNA turnover protein 4 homolog OS=Mus musculus OX=10090 GN=Mrto4 PE=1 SV=1[more]
Q6FJ043.4e-0440.82Ribosome assembly factor mrt4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / ... [more]
Q7S3023.4e-0440.82Ribosome assembly factor mrt4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-... [more]
Match NameE-valueIdentityDescription
A0A6J1I1E21.1e-2173.26Ribosome assembly factor mrt4 OS=Cucurbita maxima OX=3661 GN=LOC111468997 PE=3 S... [more]
A0A6J1F4Z21.1e-2173.26Ribosome assembly factor mrt4 OS=Cucurbita moschata OX=3662 GN=LOC111440919 PE=3... [more]
A0A6J1DT712.4e-2172.09Ribosome assembly factor mrt4 OS=Momordica charantia OX=3673 GN=LOC111024162 PE=... [more]
A0A0A0KNA71.9e-1877.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G453720 PE=3 SV=1[more]
A0A4S4DS101.1e-1655.43Ribosome assembly factor mrt4 OS=Camellia sinensis var. sinensis OX=542762 GN=TE... [more]
Match NameE-valueIdentityDescription
AT1G25260.15.2e-1650.00Ribosomal protein L10 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001790Ribosomal protein L10PPFAMPF00466Ribosomal_L10coord: 40..89
e-value: 4.3E-9
score: 36.4
IPR043141Ribosomal protein L10-like domain superfamilyGENE3D3.30.70.1730coord: 30..91
e-value: 7.0E-9
score: 37.7
IPR043141Ribosomal protein L10-like domain superfamilySUPERFAMILY160369Ribosomal protein L10-likecoord: 41..84
NoneNo IPR availablePANTHERPTHR45841:SF4RIBOSOME ASSEMBLY FACTOR MRT4coord: 30..87
NoneNo IPR availablePANTHERPTHR45841MRNA TURNOVER PROTEIN 4 MRTO4coord: 30..87

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc02G00710.2Clc02G00710.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000956 nuclear-transcribed mRNA catabolic process
biological_process GO:0042273 ribosomal large subunit biogenesis
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005730 nucleolus
cellular_component GO:0030687 preribosome, large subunit precursor