Clc02G00460 (gene) Watermelon (cordophanus) v2

Overview
NameClc02G00460
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationClcChr02: 465794 .. 467796 (+)
RNA-Seq ExpressionClc02G00460
SyntenyClc02G00460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCATTACTTGCAAAAATCTCTTTGTTCATCTCTCACTAGGATAACCTCTATTTTGTCCCTCTCTTCTGATCCTATACTGCTTTCTCACCACTTCCTGACTCTCTTTTCTGATATCTGCCAGTGTGAACACCATTAGCTCACACCAAAATCAGTAACTCCCCCATACAAAAACTCACTGTTTAAGTCTTAATATTTGCCTACTTTTCTTTCCCATCACTTCCTCTTCTTCAGCTTTTTTGCTTTCTAAATCCACCTTTACCATCTGATTCCCATTTCTGTATCAGTTTTTCATCTATCTTTATGGCCATTTTCCTCACCTTTTAGCTTTTGGGGTCTCCATTTTCCTTTTTCCTTTCCCCAAATATCACTGTTGTCTTTCTTTTGAAACTAAAAAAGAAAAGCCTTTGGGAACTCAATCGATCTCTTCCTTTTCTCTTTCTTTTGAACAACTTTCCCACCGTATCTTCCACTTCTCTCAAAGATTTTCCATATCTCTCTTTTTACCAAATTTCTAATAGGGATAGCCACTCTCTTGCTTCCCCTGGTTGTTGTTGTTAGTTTTATTCTTTTTCCTCTCCATGAAAGAAGCTGATCATGAGACCATTCCTCACTTGTTTAGATGTCCAATAAGTCTTGATTTGTTTAAGGATCCTGTGACCCTTTGCACAGGACAAACCTATGAAAGATCCAGCATTGAGAAATGGTTGGCATCAGGGAATCTCACATGTCCTGTTACAATGCAGAAGCTTCATGACCCTTCTTTTGTTCCTAATAATACTCTTCGCCATTTGATTTGCCAGTGGCTTCAGATGAGTGACCAAATAAACCCTCAATGCGTTTCTGCTATTGATTCTATGGCTGCTTTGAGAACCAAACTTGAATCTGATGAGCTTTCTTATGAATACAAGCTCCAAGTTCTCCACAGAGTTAGAATTTTATGTGAAGACTTTCCTTCTCGTAATTCTTGTTTGATCCAAATTGGGTTCTTGTCAGTGCTTTTGGAGTTGGTTTTTGGGGAAGCAGAAGCCAAACTATCACAAGAGTATCAAGAATTTGTAGAGCAAGCGCTTAGTTTTATGGTTGATGTGGTTTCTCATGATCAGATACAGTCCCTAAATTTGCTAATGGATGAGTCTAAACTATCAAGGTTCGTGGTTTTGTTCAGTCATGGCACTCCTATGATCAAGATCAGTTTGTGTCATCTTGTTGATGCAATTTCATCTTCAGTTGAAACTAAAGATCTTGGTGCTTTACTCGGAAACGCCACCGAGCTTTTGAGAGAAATCGTGCAACTTCTACACCATAACTCAGAGGCCTCCGATGCAGCAACCAAAGCCATATGTTCACTAAGTAACTTGGAAGAAAATATCAACAATTTGGTTGTGGAAGGTGCAGTTACTGGACTTATTTTATATATTTCTAATGCTCAACAACGAGAAAGAAGCTGTGCACGAGTGGCAATGGCAGCAATTGAGAAACTTTTGATACTAAGAAGTGCAAAAGAGGAAGTGGTAAATAATCCAGACGGTGTAAATGCTATTGTGAAGATGGTTTTTAGAGTATCAGATCATGGAGGCAGTGAAAGTGCTGTTAGTTCACTTATAATTTTATGTTATGATTCAGTAGAAGCAAGAGAAAAGGCCATTAGAGTTGGAGTTTTAAGTCAATTGCTTCTGCTTCTGCAGAGTCAGTGCTGTGCAAAGACCAAAACAAAGGCAAGAATCCTGCTCAAGCTTCTTAGATCCAAGTGGGTTGAGGACAAAAGGAATATGTAATGTGTGATAATAATATTCTATCCCTCAACCTTTTTGGGGCTTTTTTTCCCCTTTCAAATGTGAATCTTTACATTATAGCCTGCAAATTTGATTGGCTATAGAAGAAATATCTAGTAGGGTACATCATCAGGTATAGAAAAATTCACTCATTGGAGATTTTCAGTCACATGCAAATGCATTTCAGTATCATTATATTCAGAATGAAAGGCCACATTCTACATCTGAA

mRNA sequence

TATCATTACTTGCAAAAATCTCTTTGTTCATCTCTCACTAGGATAACCTCTATTTTGTCCCTCTCTTCTGATCCTATACTGCTTTCTCACCACTTCCTGACTCTCTTTTCTGATATCTGCCAGTGTGAACACCATTAGCTCACACCAAAATCAGTAACTCCCCCATACAAAAACTCACTGTTTAAGTCTTAATATTTGCCTACTTTTCTTTCCCATCACTTCCTCTTCTTCAGCTTTTTTGCTTTCTAAATCCACCTTTACCATCTGATTCCCATTTCTGTATCAGTTTTTCATCTATCTTTATGGCCATTTTCCTCACCTTTTAGCTTTTGGGGTCTCCATTTTCCTTTTTCCTTTCCCCAAATATCACTGTTGTCTTTCTTTTGAAACTAAAAAAGAAAAGCCTTTGGGAACTCAATCGATCTCTTCCTTTTCTCTTTCTTTTGAACAACTTTCCCACCGTATCTTCCACTTCTCTCAAAGATTTTCCATATCTCTCTTTTTACCAAATTTCTAATAGGGATAGCCACTCTCTTGCTTCCCCTGGTTGTTGTTGTTAGTTTTATTCTTTTTCCTCTCCATGAAAGAAGCTGATCATGAGACCATTCCTCACTTGTTTAGATGTCCAATAAGTCTTGATTTGTTTAAGGATCCTGTGACCCTTTGCACAGGACAAACCTATGAAAGATCCAGCATTGAGAAATGGTTGGCATCAGGGAATCTCACATGTCCTGTTACAATGCAGAAGCTTCATGACCCTTCTTTTGTTCCTAATAATACTCTTCGCCATTTGATTTGCCAGTGGCTTCAGATGAGTGACCAAATAAACCCTCAATGCGTTTCTGCTATTGATTCTATGGCTGCTTTGAGAACCAAACTTGAATCTGATGAGCTTTCTTATGAATACAAGCTCCAAGTTCTCCACAGAGTTAGAATTTTATGTGAAGACTTTCCTTCTCGTAATTCTTGTTTGATCCAAATTGGGTTCTTGTCAGTGCTTTTGGAGTTGGTTTTTGGGGAAGCAGAAGCCAAACTATCACAAGAGTATCAAGAATTTGTAGAGCAAGCGCTTAGTTTTATGGTTGATGTGGTTTCTCATGATCAGATACAGTCCCTAAATTTGCTAATGGATGAGTCTAAACTATCAAGGTTCGTGGTTTTGTTCAGTCATGGCACTCCTATGATCAAGATCAGTTTGTGTCATCTTGTTGATGCAATTTCATCTTCAGTTGAAACTAAAGATCTTGGTGCTTTACTCGGAAACGCCACCGAGCTTTTGAGAGAAATCGTGCAACTTCTACACCATAACTCAGAGGCCTCCGATGCAGCAACCAAAGCCATATGTTCACTAAGTAACTTGGAAGAAAATATCAACAATTTGGTTGTGGAAGGTGCAGTTACTGGACTTATTTTATATATTTCTAATGCTCAACAACGAGAAAGAAGCTGTGCACGAGTGGCAATGGCAGCAATTGAGAAACTTTTGATACTAAGAAGTGCAAAAGAGGAAGTGGTAAATAATCCAGACGGTGTAAATGCTATTGTGAAGATGGTTTTTAGAGTATCAGATCATGGAGGCAGTGAAAGTGCTGTTAGTTCACTTATAATTTTATGTTATGATTCAGTAGAAGCAAGAGAAAAGGCCATTAGAGTTGGAGTTTTAAGTCAATTGCTTCTGCTTCTGCAGAGTCAGTGCTGTGCAAAGACCAAAACAAAGGCAAGAATCCTGCTCAAGCTTCTTAGATCCAAGTGGGTTGAGGACAAAAGGAATATGTAATGTGTGATAATAATATTCTATCCCTCAACCTTTTTGGGGCTTTTTTTCCCCTTTCAAATGTGAATCTTTACATTATAGCCTGCAAATTTGATTGGCTATAGAAGAAATATCTAGTAGGGTACATCATCAGGTATAGAAAAATTCACTCATTGGAGATTTTCAGTCACATGCAAATGCATTTCAGTATCATTATATTCAGAATGAAAGGCCACATTCTACATCTGAA

Coding sequence (CDS)

ATGAAAGAAGCTGATCATGAGACCATTCCTCACTTGTTTAGATGTCCAATAAGTCTTGATTTGTTTAAGGATCCTGTGACCCTTTGCACAGGACAAACCTATGAAAGATCCAGCATTGAGAAATGGTTGGCATCAGGGAATCTCACATGTCCTGTTACAATGCAGAAGCTTCATGACCCTTCTTTTGTTCCTAATAATACTCTTCGCCATTTGATTTGCCAGTGGCTTCAGATGAGTGACCAAATAAACCCTCAATGCGTTTCTGCTATTGATTCTATGGCTGCTTTGAGAACCAAACTTGAATCTGATGAGCTTTCTTATGAATACAAGCTCCAAGTTCTCCACAGAGTTAGAATTTTATGTGAAGACTTTCCTTCTCGTAATTCTTGTTTGATCCAAATTGGGTTCTTGTCAGTGCTTTTGGAGTTGGTTTTTGGGGAAGCAGAAGCCAAACTATCACAAGAGTATCAAGAATTTGTAGAGCAAGCGCTTAGTTTTATGGTTGATGTGGTTTCTCATGATCAGATACAGTCCCTAAATTTGCTAATGGATGAGTCTAAACTATCAAGGTTCGTGGTTTTGTTCAGTCATGGCACTCCTATGATCAAGATCAGTTTGTGTCATCTTGTTGATGCAATTTCATCTTCAGTTGAAACTAAAGATCTTGGTGCTTTACTCGGAAACGCCACCGAGCTTTTGAGAGAAATCGTGCAACTTCTACACCATAACTCAGAGGCCTCCGATGCAGCAACCAAAGCCATATGTTCACTAAGTAACTTGGAAGAAAATATCAACAATTTGGTTGTGGAAGGTGCAGTTACTGGACTTATTTTATATATTTCTAATGCTCAACAACGAGAAAGAAGCTGTGCACGAGTGGCAATGGCAGCAATTGAGAAACTTTTGATACTAAGAAGTGCAAAAGAGGAAGTGGTAAATAATCCAGACGGTGTAAATGCTATTGTGAAGATGGTTTTTAGAGTATCAGATCATGGAGGCAGTGAAAGTGCTGTTAGTTCACTTATAATTTTATGTTATGATTCAGTAGAAGCAAGAGAAAAGGCCATTAGAGTTGGAGTTTTAAGTCAATTGCTTCTGCTTCTGCAGAGTCAGTGCTGTGCAAAGACCAAAACAAAGGCAAGAATCCTGCTCAAGCTTCTTAGATCCAAGTGGGTTGAGGACAAAAGGAATATGTAA

Protein sequence

MKEADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRILCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSLNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQLLHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Homology
BLAST of Clc02G00460 vs. NCBI nr
Match: XP_038901432.1 (U-box domain-containing protein 26-like [Benincasa hispida])

HSP 1 Score: 717.2 bits (1850), Expect = 7.7e-203
Identity = 373/399 (93.48%), Postives = 384/399 (96.24%), Query Frame = 0

Query: 1   MKEADH-ETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKEADH ETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD
Sbjct: 1   MKEADHDETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
           PSFVPNNTLRHLICQWLQMSDQINP CVSAIDSMAALRTKLESDE SYEYKLQVL RVRI
Sbjct: 61  PSFVPNNTLRHLICQWLQMSDQINPHCVSAIDSMAALRTKLESDEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE+F SRNSCLIQ GFLSVLLELVFGEAEAKLS+EYQEFVE ALSFMV VVS DQIQSL
Sbjct: 121 LCEEFSSRNSCLIQFGFLSVLLELVFGEAEAKLSKEYQEFVEHALSFMVAVVSDDQIQSL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LMDESKLSRF+VLFSHGTPMIKISLCHLV+ ISSSV+TKDLGALLGN TELLREIVQL
Sbjct: 181 NVLMDESKLSRFMVLFSHGTPMIKISLCHLVEVISSSVKTKDLGALLGNTTELLREIVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           LHHNSEASDAATKAICSLSNLE+NINNLVVEGAVTGLI YISNAQQR+RS A+VAMAAIE
Sbjct: 241 LHHNSEASDAATKAICSLSNLEQNINNLVVEGAVTGLISYISNAQQRDRSSAQVAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL+LRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSE+AV SLIILCYDSVEAREKAIR G
Sbjct: 301 KLLVLRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSENAVGSLIILCYDSVEAREKAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. NCBI nr
Match: XP_008460887.1 (PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0040754.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa] >TYK02069.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa])

HSP 1 Score: 705.7 bits (1820), Expect = 2.3e-199
Identity = 369/399 (92.48%), Postives = 379/399 (94.99%), Query Frame = 0

Query: 1   MKEA-DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKEA DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKL D
Sbjct: 1   MKEADDHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLQD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
           PSFVPNNTLRHLICQWLQMSDQINPQCVS IDSMAALR KLESDE SYEYKLQVL RVRI
Sbjct: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSTIDSMAALRIKLESDEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE+FPSRNSCLIQIGFLSVLLEL+FG+ EAKLSQEYQEFVEQALSFMV +VS +QIQ L
Sbjct: 121 LCEEFPSRNSCLIQIGFLSVLLELIFGQEEAKLSQEYQEFVEQALSFMVAMVSLEQIQPL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LMDESKLSRF+VLFS+GT MIKISLCHLV AISSSVETKDL  LLGN TELLREIVQL
Sbjct: 181 NVLMDESKLSRFMVLFSNGTSMIKISLCHLVQAISSSVETKDLSVLLGNTTELLREIVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           LHHNSEASDAATKAICSLSNLE+NINNLVVEGAVTGLI YISNAQQRERS AR AMAAIE
Sbjct: 241 LHHNSEASDAATKAICSLSNLEQNINNLVVEGAVTGLISYISNAQQRERSSAREAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL+LRSAKEEVVNNPDGVN IVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIR G
Sbjct: 301 KLLVLRSAKEEVVNNPDGVNVIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. NCBI nr
Match: XP_004147364.1 (U-box domain-containing protein 26 [Cucumis sativus] >KAE8651985.1 hypothetical protein Csa_016923 [Cucumis sativus])

HSP 1 Score: 702.6 bits (1812), Expect = 2.0e-198
Identity = 367/399 (91.98%), Postives = 378/399 (94.74%), Query Frame = 0

Query: 1   MKEA-DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKEA DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD
Sbjct: 1   MKEADDHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
           PSFVPNNTLRHLICQWLQMSDQINPQCVS IDSMAALR KLESDE SYEYKLQVL RVRI
Sbjct: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSTIDSMAALRIKLESDEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE+FPSRNSCLI+IGFLSVLLEL+FG+ E KLSQEYQEFVEQALSFMV +VS +QIQSL
Sbjct: 121 LCEEFPSRNSCLIRIGFLSVLLELIFGQEETKLSQEYQEFVEQALSFMVAMVSLEQIQSL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LMDESKLSRF+VLFS+GT MIKISLCHLV AISSSVETKDL  LLGN TELLREIVQL
Sbjct: 181 NVLMDESKLSRFMVLFSNGTSMIKISLCHLVQAISSSVETKDLSVLLGNTTELLREIVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           LHHNSEASDAATKAICSLSNLE+NINNLVVEGAVTGLI YISNAQQRERS AR AMAAIE
Sbjct: 241 LHHNSEASDAATKAICSLSNLEQNINNLVVEGAVTGLISYISNAQQRERSSAREAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL+ RSAKEEVVN PDGVN IVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIR G
Sbjct: 301 KLLVFRSAKEEVVNIPDGVNVIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. NCBI nr
Match: XP_022971624.1 (U-box domain-containing protein 26-like [Cucurbita maxima])

HSP 1 Score: 686.4 bits (1770), Expect = 1.4e-193
Identity = 356/398 (89.45%), Postives = 374/398 (93.97%), Query Frame = 0

Query: 1   MKEADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDP 60
           MKEA HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSI+KWLASGNLTCPVTMQKLHDP
Sbjct: 1   MKEAAHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIQKWLASGNLTCPVTMQKLHDP 60

Query: 61  SFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRIL 120
           SFVPNNTLRHLI QWLQM DQI+P CVSAIDSMAALRTKLES E SYEYKLQVL RVRIL
Sbjct: 61  SFVPNNTLRHLISQWLQMGDQISPHCVSAIDSMAALRTKLESHEFSYEYKLQVLQRVRIL 120

Query: 121 CEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSLN 180
           CE FPSRNSC +Q GFLSVLLELVFGEAEAKLSQEYQEFVEQALSF+V +VS DQIQSLN
Sbjct: 121 CEQFPSRNSCFVQFGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFVVSMVSLDQIQSLN 180

Query: 181 LLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQLL 240
           +LM+ESKLSRF+VLFSHGT  IKISLC LV+AISSSV+TKDLG LLGNATELLRE+VQLL
Sbjct: 181 MLMEESKLSRFMVLFSHGTATIKISLCQLVEAISSSVQTKDLGVLLGNATELLREVVQLL 240

Query: 241 HHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIEK 300
            HNSE SDAATKAICSLSN+E++INNLVVEGAVTGLI YISNAQQRERS ARVAMAAIEK
Sbjct: 241 RHNSEVSDAATKAICSLSNMEQHINNLVVEGAVTGLITYISNAQQRERSSARVAMAAIEK 300

Query: 301 LLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVGV 360
           LL++RSAK EVVNNP+GVNAIVKMVFRVSD GGSESAV SLIILCYDSVEARE+AIR GV
Sbjct: 301 LLVMRSAKVEVVNNPNGVNAIVKMVFRVSDLGGSESAVGSLIILCYDSVEAREEAIRGGV 360

Query: 361 LSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           LSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 LSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 398

BLAST of Clc02G00460 vs. NCBI nr
Match: XP_023512357.1 (U-box domain-containing protein 26-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.9 bits (1748), Expect = 5.2e-191
Identity = 353/399 (88.47%), Postives = 373/399 (93.48%), Query Frame = 0

Query: 1   MKEAD-HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKEA  HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSI+KWLASGNLTCPVTMQKLHD
Sbjct: 1   MKEAGAHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIQKWLASGNLTCPVTMQKLHD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
           PSFVPNNTLRHLI QWLQM DQI+P CVSAIDSMAALRTKLES E SYEYKLQVL RVRI
Sbjct: 61  PSFVPNNTLRHLISQWLQMGDQISPHCVSAIDSMAALRTKLESHEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE FPSRNSC +Q GFLSVLLELVFGEAEAKLSQEYQEFVEQALSF+V +VS DQIQSL
Sbjct: 121 LCEQFPSRNSCFVQFGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFVVSMVSLDQIQSL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LM+ESKLSRF+VLFSHGT  IKISLC LV+AISSSV+TKDLG LLGNATELLRE+VQL
Sbjct: 181 NMLMEESKLSRFMVLFSHGTATIKISLCQLVEAISSSVQTKDLGVLLGNATELLREVVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           L HNSE SDAATKAICSLSN+E++INNLVVEGAVTGLI YISNAQQRERS ARVAMAAIE
Sbjct: 241 LRHNSEVSDAATKAICSLSNMEQHINNLVVEGAVTGLITYISNAQQRERSSARVAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL++RSAK EVVNNP+GVN IVKMVFRVSD GGSESAV SLI+LCY+SVEARE+AIR G
Sbjct: 301 KLLVMRSAKVEVVNNPNGVNVIVKMVFRVSDLGGSESAVGSLIMLCYESVEAREEAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.6e-38
Identity = 115/398 (28.89%), Postives = 196/398 (49.25%), Query Frame = 0

Query: 9   IPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNTL 68
           IP+ FRCPISLDL  DPVT+ TGQTY+R+SI+ W+A GN TCPVT   L D + +PN+TL
Sbjct: 14  IPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNHTL 73

Query: 69  RHLICQWL---------QMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 128
           R LI +W          ++     P    ++ S+ +  + +    +S   +   + R+R 
Sbjct: 74  RRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRLRG 133

Query: 129 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 188
           L  D       +       +L+ ++F + E   +    E V ++L+ +V ++   + +  
Sbjct: 134 LARDSEKNRVLIAGHNAREILVRILFADIET--TSLSSELVSESLALLV-LLHMTETECE 193

Query: 189 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 248
            +  D S++     L    +  I+++   L++ + +  ++ DL  ++  +  +   ++ L
Sbjct: 194 AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLDL 253

Query: 249 LHH---NSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMA 308
           L +   +  A     KAI +L  L +   +L +     G IL    A   +R      +A
Sbjct: 254 LKNPISSRRALKIGIKAIFALC-LVKQTRHLAISAGAPG-ILIDRLAADFDRCDTERGLA 313

Query: 309 AIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAI 368
            +E L  L         +   V  +VK + RVSD   +E A  +L+ LC      R++A 
Sbjct: 314 TVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDR-ATEYAAGALLALCTAEERCRDEAA 373

Query: 369 RVGVLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVED 395
             G+++QLLLL+QS C  + K KA++LLKLLR  W +D
Sbjct: 374 AAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDD 405

BLAST of Clc02G00460 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.1e-37
Identity = 112/396 (28.28%), Postives = 195/396 (49.24%), Query Frame = 0

Query: 9   IPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASG-NLTCPVTMQKLHDPSFVPNNT 68
           IP+ FRCPISL+L +DPVT+CTGQTY+R+SIE W++ G N TCPVT   L D + +PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 69  LRHLICQWL---------QMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVR 128
           LR LI +W          ++     P   +++ ++ +  + +    +S   +   L R+R
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLR 133

Query: 129 ILCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQS 188
               D       +       +L++++F E  +       E V ++L+ +V +   +  Q 
Sbjct: 134 GFARDSDKNRVLIAAHNATEILIKILFSETTS------SELVSESLALLVMLPITEPNQF 193

Query: 189 LNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQ 248
           +++  D  ++     L    +   +++   L++ +S+  ++ DL   + N+  +   ++ 
Sbjct: 194 VSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLD 253

Query: 249 LLHH---NSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAM 308
           LL +   +  A     K + +L +++   +  +  GA    IL    A   +R     A+
Sbjct: 254 LLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPE--ILIDRLAADFDRCDTERAL 313

Query: 309 AAIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKA 368
           A +E L            +   V  +VK + RVSD   +E A  +L+ LC      RE+A
Sbjct: 314 ATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR-ATEYAAGALLALCTAEERWREEA 373

Query: 369 IRVGVLSQLLLLLQSQCCAKTKTKARILLKLLRSKW 392
              GV+ QLLL++QS+C  + K KA+ LLKLLR  W
Sbjct: 374 AGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Clc02G00460 vs. ExPASy Swiss-Prot
Match: Q9FLF4 (U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 4.0e-29
Identity = 121/407 (29.73%), Postives = 198/407 (48.65%), Query Frame = 0

Query: 8   TIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNT 67
           T+P  FRCPISLD+ K PV+LCTG TY+R+SI++WL  GN TCP TMQ L +  F+PN T
Sbjct: 9   TVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQNKDFIPNRT 68

Query: 68  LRHLICQWLQMSDQINPQ-CVSAIDSMAAL---------RTKLESDELSYEYKLQVLHRV 127
           L+ LI  W   SD +  + CV + +  A           R K+E +E      L  +  V
Sbjct: 69  LQRLIEIW---SDSVRRRTCVESAELAAPTRDEIADAIDRVKIEKEERDDREVLSKI--V 128

Query: 128 RILCEDFPSRNSCLIQIGFLSVLLELV----FGEAEAKLSQEYQEFVEQALSFMVDVVSH 187
           R   E   +R     +  F+ +L++L+    F    A  S   QE V+   +    V   
Sbjct: 129 RFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRSKVSDR 188

Query: 188 DQIQSLNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELL 247
            +  +L L     +LS  V LF  G   +KI    L++ I+   E+K    L+     L+
Sbjct: 189 RRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESK---LLIAERDGLI 248

Query: 248 REIVQLLHHNSEAS--DAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCA 307
            E+++ +  +S+ S  +++   + ++S+ +    NL+ E  +  +   +S+      S +
Sbjct: 249 TELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSD------STS 308

Query: 308 RVAMAAIEKLL----ILRSAKE--EVVNNPDG--VNAIVKMVFRVSDHGGSESAVSSL-- 367
            ++++  EK L    IL S KE    +   DG  +  +VK + +VS    +E AV+ L  
Sbjct: 309 SLSVSVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVST-AATEHAVTVLWS 368

Query: 368 IILCYDSVEAREKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKLLR 389
           +   +   +A E    V  ++++LLLLQS C    +     LLK+ +
Sbjct: 369 VSYLFKEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFK 400

BLAST of Clc02G00460 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 5.8e-28
Identity = 112/399 (28.07%), Postives = 197/399 (49.37%), Query Frame = 0

Query: 1   MKEADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDP 60
           +KE D + IP +F CPISL+  +DPVTLCTGQTYER +I KW   G+LTCP TMQ+L D 
Sbjct: 56  IKELDLQDIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDD 115

Query: 61  SFVPNNTLRHLICQWL--------QMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQ 120
           +  PN TL HLI  W         + S+ +  + +  + ++   + +     LS   ++ 
Sbjct: 116 TVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIV 175

Query: 121 VLHRVRILCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVS 180
           + H +             +++ G +SV+  L+       +  E       A+   +D+ S
Sbjct: 176 IAHLM---------ARKTVVEEGGVSVISSLLGPFTSHAVGSEV-----VAILVSLDLDS 235

Query: 181 HDQIQSLNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATEL 240
                S + LM  +K+S  V + + G+   KI+   L+  +   VE K   A L ++  L
Sbjct: 236 ----DSKSGLMQPAKVSLIVDMLNDGSNETKINCARLIRGL---VEEKGFRAELVSSHSL 295

Query: 241 LREIVQLL---HHNSEASDAATKAICSLSNLEENINNLVVE-GAVTGLILYISNAQQRER 300
           L  +++L+    H +  S A    +    ++ + + +L+V  GAV  L+  + +    + 
Sbjct: 296 LVGLMRLVKDKRHRNGVSPAL--RLLKPISVHKQVRSLMVSIGAVPQLVDILPSL---DP 355

Query: 301 SCARVAMAAIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILC-YD 360
            C  +A+  ++ L      +  V ++ + +   V+++ RVS++  +  A+S L  +C   
Sbjct: 356 ECLELALFVLDALCTDVEGRVAVKDSANTIPYTVRVLMRVSEN-CTNYALSILWSVCKLA 415

Query: 361 SVEAREKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKL 387
             E    A+ VG+ ++LLL++QS C A  K ++  LLKL
Sbjct: 416 PEECSPLAVEVGLAAKLLLVIQSGCDAALKQRSAELLKL 427

BLAST of Clc02G00460 vs. ExPASy Swiss-Prot
Match: Q9LSA6 (U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-26
Identity = 106/397 (26.70%), Postives = 192/397 (48.36%), Query Frame = 0

Query: 8   TIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNT 67
           T+P  F+CPISLD+ + PV+LCTG TY+R+SI++WL  GN TCP TMQ L    FVPN T
Sbjct: 11  TVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKDFVPNLT 70

Query: 68  LRHLICQWLQM--------SDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 127
           L+ LI  W           S  +NP     + +   +   LE   +S E  +++   VR 
Sbjct: 71  LQRLINIWSDSIGRRHNGDSPVLNPPSGREVPTKEEVNVLLER-LMSLENLMKI---VRF 130

Query: 128 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDV-VSHDQIQS 187
           + +   +R     ++ F+ +L++++      +  +   E V  A+  +  + V  +++ +
Sbjct: 131 VKDSDSNREFLSKKMEFVPMLVDII------RTKKTKIELVIMAIRILDSIKVDRERLSN 190

Query: 188 LNLLMDESK-LSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALL-GNATELLREI 247
           L L  D    L+  ++    G    KI    ++D IS   ++K + A   G  TE+++ I
Sbjct: 191 LMLANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMKSI 250

Query: 248 VQLLHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMA 307
                 +    +A+   + ++S  +   + L+   A+T +   +        +    ++ 
Sbjct: 251 SITESSDPSLIEASLSFLITISKSKRVRSKLIAAKAITKIKDILLTETLTNVAVTEKSLK 310

Query: 308 AIEKLLILRSAKEEVVNNPDG--VNAIVKMVFRVSDHGGSESAVSSLIILCY---DSVEA 367
            +E L   R  + E+  + +G  V  +VK + +VS    +E AV+ L  LCY   +    
Sbjct: 311 LLETLSSKREGRLEICGDDNGRCVEGVVKKLLKVST-TATEHAVTILWCLCYVFREDKTV 370

Query: 368 REKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKLLR 389
            E   R   +++LL+++QS C A  +  A+ L+K+L+
Sbjct: 371 EETVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLK 396

BLAST of Clc02G00460 vs. ExPASy TrEMBL
Match: A0A5A7TBB3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00540 PE=4 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.1e-199
Identity = 369/399 (92.48%), Postives = 379/399 (94.99%), Query Frame = 0

Query: 1   MKEA-DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKEA DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKL D
Sbjct: 1   MKEADDHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLQD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
           PSFVPNNTLRHLICQWLQMSDQINPQCVS IDSMAALR KLESDE SYEYKLQVL RVRI
Sbjct: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSTIDSMAALRIKLESDEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE+FPSRNSCLIQIGFLSVLLEL+FG+ EAKLSQEYQEFVEQALSFMV +VS +QIQ L
Sbjct: 121 LCEEFPSRNSCLIQIGFLSVLLELIFGQEEAKLSQEYQEFVEQALSFMVAMVSLEQIQPL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LMDESKLSRF+VLFS+GT MIKISLCHLV AISSSVETKDL  LLGN TELLREIVQL
Sbjct: 181 NVLMDESKLSRFMVLFSNGTSMIKISLCHLVQAISSSVETKDLSVLLGNTTELLREIVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           LHHNSEASDAATKAICSLSNLE+NINNLVVEGAVTGLI YISNAQQRERS AR AMAAIE
Sbjct: 241 LHHNSEASDAATKAICSLSNLEQNINNLVVEGAVTGLISYISNAQQRERSSAREAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL+LRSAKEEVVNNPDGVN IVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIR G
Sbjct: 301 KLLVLRSAKEEVVNNPDGVNVIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. ExPASy TrEMBL
Match: A0A1S3CDG2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499628 PE=4 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.1e-199
Identity = 369/399 (92.48%), Postives = 379/399 (94.99%), Query Frame = 0

Query: 1   MKEA-DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKEA DHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKL D
Sbjct: 1   MKEADDHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLQD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
           PSFVPNNTLRHLICQWLQMSDQINPQCVS IDSMAALR KLESDE SYEYKLQVL RVRI
Sbjct: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSTIDSMAALRIKLESDEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE+FPSRNSCLIQIGFLSVLLEL+FG+ EAKLSQEYQEFVEQALSFMV +VS +QIQ L
Sbjct: 121 LCEEFPSRNSCLIQIGFLSVLLELIFGQEEAKLSQEYQEFVEQALSFMVAMVSLEQIQPL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LMDESKLSRF+VLFS+GT MIKISLCHLV AISSSVETKDL  LLGN TELLREIVQL
Sbjct: 181 NVLMDESKLSRFMVLFSNGTSMIKISLCHLVQAISSSVETKDLSVLLGNTTELLREIVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           LHHNSEASDAATKAICSLSNLE+NINNLVVEGAVTGLI YISNAQQRERS AR AMAAIE
Sbjct: 241 LHHNSEASDAATKAICSLSNLEQNINNLVVEGAVTGLISYISNAQQRERSSAREAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL+LRSAKEEVVNNPDGVN IVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIR G
Sbjct: 301 KLLVLRSAKEEVVNNPDGVNVIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. ExPASy TrEMBL
Match: A0A6J1I938 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470306 PE=4 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 7.0e-194
Identity = 356/398 (89.45%), Postives = 374/398 (93.97%), Query Frame = 0

Query: 1   MKEADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDP 60
           MKEA HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSI+KWLASGNLTCPVTMQKLHDP
Sbjct: 1   MKEAAHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIQKWLASGNLTCPVTMQKLHDP 60

Query: 61  SFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRIL 120
           SFVPNNTLRHLI QWLQM DQI+P CVSAIDSMAALRTKLES E SYEYKLQVL RVRIL
Sbjct: 61  SFVPNNTLRHLISQWLQMGDQISPHCVSAIDSMAALRTKLESHEFSYEYKLQVLQRVRIL 120

Query: 121 CEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSLN 180
           CE FPSRNSC +Q GFLSVLLELVFGEAEAKLSQEYQEFVEQALSF+V +VS DQIQSLN
Sbjct: 121 CEQFPSRNSCFVQFGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFVVSMVSLDQIQSLN 180

Query: 181 LLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQLL 240
           +LM+ESKLSRF+VLFSHGT  IKISLC LV+AISSSV+TKDLG LLGNATELLRE+VQLL
Sbjct: 181 MLMEESKLSRFMVLFSHGTATIKISLCQLVEAISSSVQTKDLGVLLGNATELLREVVQLL 240

Query: 241 HHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIEK 300
            HNSE SDAATKAICSLSN+E++INNLVVEGAVTGLI YISNAQQRERS ARVAMAAIEK
Sbjct: 241 RHNSEVSDAATKAICSLSNMEQHINNLVVEGAVTGLITYISNAQQRERSSARVAMAAIEK 300

Query: 301 LLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVGV 360
           LL++RSAK EVVNNP+GVNAIVKMVFRVSD GGSESAV SLIILCYDSVEARE+AIR GV
Sbjct: 301 LLVMRSAKVEVVNNPNGVNAIVKMVFRVSDLGGSESAVGSLIILCYDSVEAREEAIRGGV 360

Query: 361 LSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           LSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 LSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 398

BLAST of Clc02G00460 vs. ExPASy TrEMBL
Match: A0A6J1EVV1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111438339 PE=4 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 6.1e-190
Identity = 353/399 (88.47%), Postives = 370/399 (92.73%), Query Frame = 0

Query: 1   MKE-ADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHD 60
           MKE A HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSI+KWLASGNLTCPVTMQKLHD
Sbjct: 1   MKEAAAHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIQKWLASGNLTCPVTMQKLHD 60

Query: 61  PSFVPNNTLRHLICQWLQMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 120
            SFVPNNTLRHLI QWLQM DQI+P CVSAIDSMAALRTKLES E SYEYKLQVL RVRI
Sbjct: 61  TSFVPNNTLRHLISQWLQMGDQISPHCVSAIDSMAALRTKLESHEFSYEYKLQVLQRVRI 120

Query: 121 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 180
           LCE FPSRNSC +Q GFLSVLLELVFGE EAKLSQEYQEFVEQALSF+V +VS DQIQSL
Sbjct: 121 LCEQFPSRNSCFVQFGFLSVLLELVFGEEEAKLSQEYQEFVEQALSFVVSMVSLDQIQSL 180

Query: 181 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 240
           N+LM+E KLSRF+VLFSHGT  IKISLC LV+AISSSV+TKDLG LLGNATELLRE+VQL
Sbjct: 181 NMLMEEPKLSRFMVLFSHGTATIKISLCQLVEAISSSVQTKDLGVLLGNATELLREVVQL 240

Query: 241 LHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAIE 300
           L HNSE SDAATKAICSLSN+E++INNLVVEGAVTGLI YISNAQQRERS ARVAMAAIE
Sbjct: 241 LRHNSEVSDAATKAICSLSNMEQHINNLVVEGAVTGLITYISNAQQRERSSARVAMAAIE 300

Query: 301 KLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRVG 360
           KLL +RSAK EVVNNP+GVNAIVKMVFRVSD GGSESAV SLIILCYDSVEARE+AIR G
Sbjct: 301 KLLAMRSAKVEVVNNPNGVNAIVKMVFRVSDLGGSESAVGSLIILCYDSVEAREEAIRGG 360

Query: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 VLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. ExPASy TrEMBL
Match: A0A6J1DU80 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111023521 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.2e-182
Identity = 340/400 (85.00%), Postives = 369/400 (92.25%), Query Frame = 0

Query: 1   MKEADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDP 60
           MKEA HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLA+GNLTCPVTMQKL+DP
Sbjct: 1   MKEA-HETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLATGNLTCPVTMQKLNDP 60

Query: 61  SFVPNNTLRHLICQWLQM--SDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVR 120
           SFVPNN LRHLI QWLQM   DQINP C+SAIDS+AALR+KLES+E S+ YKLQVL RVR
Sbjct: 61  SFVPNNNLRHLITQWLQMGDGDQINPHCLSAIDSLAALRSKLESNEFSFGYKLQVLQRVR 120

Query: 121 ILCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQS 180
           IL E+FPS NSCL+Q GFLSVLLELVFG+ +AKLSQEYQEFVE+AL F+V +VS  Q+QS
Sbjct: 121 ILSEEFPSCNSCLVQFGFLSVLLELVFGQTDAKLSQEYQEFVERALGFVVTMVSLGQLQS 180

Query: 181 LNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQ 240
           LN+LM+ESKLSRF+ LF+HGTPMIKISLCHLV+AISSSVETKDLGALLGNATELL EIV+
Sbjct: 181 LNVLMEESKLSRFIDLFNHGTPMIKISLCHLVEAISSSVETKDLGALLGNATELLHEIVR 240

Query: 241 LLHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMAAI 300
           LLH +SEASDAATKAI SLSNLE+N  NLV +GAV GLI YISNAQ+RERS ARVAMAAI
Sbjct: 241 LLHQSSEASDAATKAIFSLSNLEQNAINLVADGAVAGLISYISNAQRRERSSARVAMAAI 300

Query: 301 EKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAIRV 360
           EKLL++RSAKEEVVN+ DGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEARE+AIR 
Sbjct: 301 EKLLVIRSAKEEVVNSTDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREEAIRG 360

Query: 361 GVLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVEDKRNM 399
           GVLSQLLLLLQSQCC KTKTKARILLKLLRSKWVEDKRNM
Sbjct: 361 GVLSQLLLLLQSQCCVKTKTKARILLKLLRSKWVEDKRNM 399

BLAST of Clc02G00460 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-39
Identity = 115/398 (28.89%), Postives = 196/398 (49.25%), Query Frame = 0

Query: 9   IPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNTL 68
           IP+ FRCPISLDL  DPVT+ TGQTY+R+SI+ W+A GN TCPVT   L D + +PN+TL
Sbjct: 14  IPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNHTL 73

Query: 69  RHLICQWL---------QMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 128
           R LI +W          ++     P    ++ S+ +  + +    +S   +   + R+R 
Sbjct: 74  RRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRLRG 133

Query: 129 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQSL 188
           L  D       +       +L+ ++F + E   +    E V ++L+ +V ++   + +  
Sbjct: 134 LARDSEKNRVLIAGHNAREILVRILFADIET--TSLSSELVSESLALLV-LLHMTETECE 193

Query: 189 NLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQL 248
            +  D S++     L    +  I+++   L++ + +  ++ DL  ++  +  +   ++ L
Sbjct: 194 AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLDL 253

Query: 249 LHH---NSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMA 308
           L +   +  A     KAI +L  L +   +L +     G IL    A   +R      +A
Sbjct: 254 LKNPISSRRALKIGIKAIFALC-LVKQTRHLAISAGAPG-ILIDRLAADFDRCDTERGLA 313

Query: 309 AIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKAI 368
            +E L  L         +   V  +VK + RVSD   +E A  +L+ LC      R++A 
Sbjct: 314 TVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDR-ATEYAAGALLALCTAEERCRDEAA 373

Query: 369 RVGVLSQLLLLLQSQCCAKTKTKARILLKLLRSKWVED 395
             G+++QLLLL+QS C  + K KA++LLKLLR  W +D
Sbjct: 374 AAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDD 405

BLAST of Clc02G00460 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 157.5 bits (397), Expect = 2.2e-38
Identity = 112/396 (28.28%), Postives = 195/396 (49.24%), Query Frame = 0

Query: 9   IPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASG-NLTCPVTMQKLHDPSFVPNNT 68
           IP+ FRCPISL+L +DPVT+CTGQTY+R+SIE W++ G N TCPVT   L D + +PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 69  LRHLICQWL---------QMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVR 128
           LR LI +W          ++     P   +++ ++ +  + +    +S   +   L R+R
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLR 133

Query: 129 ILCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVSHDQIQS 188
               D       +       +L++++F E  +       E V ++L+ +V +   +  Q 
Sbjct: 134 GFARDSDKNRVLIAAHNATEILIKILFSETTS------SELVSESLALLVMLPITEPNQF 193

Query: 189 LNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELLREIVQ 248
           +++  D  ++     L    +   +++   L++ +S+  ++ DL   + N+  +   ++ 
Sbjct: 194 VSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLD 253

Query: 249 LLHH---NSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAM 308
           LL +   +  A     K + +L +++   +  +  GA    IL    A   +R     A+
Sbjct: 254 LLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPE--ILIDRLAADFDRCDTERAL 313

Query: 309 AAIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILCYDSVEAREKA 368
           A +E L            +   V  +VK + RVSD   +E A  +L+ LC      RE+A
Sbjct: 314 ATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR-ATEYAAGALLALCTAEERWREEA 373

Query: 369 IRVGVLSQLLLLLQSQCCAKTKTKARILLKLLRSKW 392
              GV+ QLLL++QS+C  + K KA+ LLKLLR  W
Sbjct: 374 AGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Clc02G00460 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 130.6 bits (327), Expect = 2.9e-30
Identity = 121/407 (29.73%), Postives = 198/407 (48.65%), Query Frame = 0

Query: 8   TIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNT 67
           T+P  FRCPISLD+ K PV+LCTG TY+R+SI++WL  GN TCP TMQ L +  F+PN T
Sbjct: 9   TVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQNKDFIPNRT 68

Query: 68  LRHLICQWLQMSDQINPQ-CVSAIDSMAAL---------RTKLESDELSYEYKLQVLHRV 127
           L+ LI  W   SD +  + CV + +  A           R K+E +E      L  +  V
Sbjct: 69  LQRLIEIW---SDSVRRRTCVESAELAAPTRDEIADAIDRVKIEKEERDDREVLSKI--V 128

Query: 128 RILCEDFPSRNSCLIQIGFLSVLLELV----FGEAEAKLSQEYQEFVEQALSFMVDVVSH 187
           R   E   +R     +  F+ +L++L+    F    A  S   QE V+   +    V   
Sbjct: 129 RFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRSKVSDR 188

Query: 188 DQIQSLNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATELL 247
            +  +L L     +LS  V LF  G   +KI    L++ I+   E+K    L+     L+
Sbjct: 189 RRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESK---LLIAERDGLI 248

Query: 248 REIVQLLHHNSEAS--DAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCA 307
            E+++ +  +S+ S  +++   + ++S+ +    NL+ E  +  +   +S+      S +
Sbjct: 249 TELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSD------STS 308

Query: 308 RVAMAAIEKLL----ILRSAKE--EVVNNPDG--VNAIVKMVFRVSDHGGSESAVSSL-- 367
            ++++  EK L    IL S KE    +   DG  +  +VK + +VS    +E AV+ L  
Sbjct: 309 SLSVSVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVST-AATEHAVTVLWS 368

Query: 368 IILCYDSVEAREKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKLLR 389
           +   +   +A E    V  ++++LLLLQS C    +     LLK+ +
Sbjct: 369 VSYLFKEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFK 400

BLAST of Clc02G00460 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 4.1e-29
Identity = 112/399 (28.07%), Postives = 197/399 (49.37%), Query Frame = 0

Query: 1   MKEADHETIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDP 60
           +KE D + IP +F CPISL+  +DPVTLCTGQTYER +I KW   G+LTCP TMQ+L D 
Sbjct: 56  IKELDLQDIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDD 115

Query: 61  SFVPNNTLRHLICQWL--------QMSDQINPQCVSAIDSMAALRTKLESDELSYEYKLQ 120
           +  PN TL HLI  W         + S+ +  + +  + ++   + +     LS   ++ 
Sbjct: 116 TVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIV 175

Query: 121 VLHRVRILCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDVVS 180
           + H +             +++ G +SV+  L+       +  E       A+   +D+ S
Sbjct: 176 IAHLM---------ARKTVVEEGGVSVISSLLGPFTSHAVGSEV-----VAILVSLDLDS 235

Query: 181 HDQIQSLNLLMDESKLSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALLGNATEL 240
                S + LM  +K+S  V + + G+   KI+   L+  +   VE K   A L ++  L
Sbjct: 236 ----DSKSGLMQPAKVSLIVDMLNDGSNETKINCARLIRGL---VEEKGFRAELVSSHSL 295

Query: 241 LREIVQLL---HHNSEASDAATKAICSLSNLEENINNLVVE-GAVTGLILYISNAQQRER 300
           L  +++L+    H +  S A    +    ++ + + +L+V  GAV  L+  + +    + 
Sbjct: 296 LVGLMRLVKDKRHRNGVSPAL--RLLKPISVHKQVRSLMVSIGAVPQLVDILPSL---DP 355

Query: 301 SCARVAMAAIEKLLILRSAKEEVVNNPDGVNAIVKMVFRVSDHGGSESAVSSLIILC-YD 360
            C  +A+  ++ L      +  V ++ + +   V+++ RVS++  +  A+S L  +C   
Sbjct: 356 ECLELALFVLDALCTDVEGRVAVKDSANTIPYTVRVLMRVSEN-CTNYALSILWSVCKLA 415

Query: 361 SVEAREKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKL 387
             E    A+ VG+ ++LLL++QS C A  K ++  LLKL
Sbjct: 416 PEECSPLAVEVGLAAKLLLVIQSGCDAALKQRSAELLKL 427

BLAST of Clc02G00460 vs. TAIR 10
Match: AT3G18710.1 (plant U-box 29 )

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-27
Identity = 106/397 (26.70%), Postives = 192/397 (48.36%), Query Frame = 0

Query: 8   TIPHLFRCPISLDLFKDPVTLCTGQTYERSSIEKWLASGNLTCPVTMQKLHDPSFVPNNT 67
           T+P  F+CPISLD+ + PV+LCTG TY+R+SI++WL  GN TCP TMQ L    FVPN T
Sbjct: 11  TVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKDFVPNLT 70

Query: 68  LRHLICQWLQM--------SDQINPQCVSAIDSMAALRTKLESDELSYEYKLQVLHRVRI 127
           L+ LI  W           S  +NP     + +   +   LE   +S E  +++   VR 
Sbjct: 71  LQRLINIWSDSIGRRHNGDSPVLNPPSGREVPTKEEVNVLLER-LMSLENLMKI---VRF 130

Query: 128 LCEDFPSRNSCLIQIGFLSVLLELVFGEAEAKLSQEYQEFVEQALSFMVDV-VSHDQIQS 187
           + +   +R     ++ F+ +L++++      +  +   E V  A+  +  + V  +++ +
Sbjct: 131 VKDSDSNREFLSKKMEFVPMLVDII------RTKKTKIELVIMAIRILDSIKVDRERLSN 190

Query: 188 LNLLMDESK-LSRFVVLFSHGTPMIKISLCHLVDAISSSVETKDLGALL-GNATELLREI 247
           L L  D    L+  ++    G    KI    ++D IS   ++K + A   G  TE+++ I
Sbjct: 191 LMLANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMKSI 250

Query: 248 VQLLHHNSEASDAATKAICSLSNLEENINNLVVEGAVTGLILYISNAQQRERSCARVAMA 307
                 +    +A+   + ++S  +   + L+   A+T +   +        +    ++ 
Sbjct: 251 SITESSDPSLIEASLSFLITISKSKRVRSKLIAAKAITKIKDILLTETLTNVAVTEKSLK 310

Query: 308 AIEKLLILRSAKEEVVNNPDG--VNAIVKMVFRVSDHGGSESAVSSLIILCY---DSVEA 367
            +E L   R  + E+  + +G  V  +VK + +VS    +E AV+ L  LCY   +    
Sbjct: 311 LLETLSSKREGRLEICGDDNGRCVEGVVKKLLKVST-TATEHAVTILWCLCYVFREDKTV 370

Query: 368 REKAIRVGVLSQLLLLLQSQCCAKTKTKARILLKLLR 389
            E   R   +++LL+++QS C A  +  A+ L+K+L+
Sbjct: 371 EETVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLK 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901432.17.7e-20393.48U-box domain-containing protein 26-like [Benincasa hispida][more]
XP_008460887.12.3e-19992.48PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0040754.1 ... [more]
XP_004147364.12.0e-19891.98U-box domain-containing protein 26 [Cucumis sativus] >KAE8651985.1 hypothetical ... [more]
XP_022971624.11.4e-19389.45U-box domain-containing protein 26-like [Cucurbita maxima][more]
XP_023512357.15.2e-19188.47U-box domain-containing protein 26-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FXA41.6e-3828.89U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9LT793.1e-3728.28U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FLF44.0e-2929.73U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2... [more]
Q058P45.8e-2828.07U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1... [more]
Q9LSA61.4e-2626.70U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5A7TBB31.1e-19992.48RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CDG21.1e-19992.48RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499628 PE=4 ... [more]
A0A6J1I9387.0e-19489.45RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470306 P... [more]
A0A6J1EVV16.1e-19088.47RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111438339... [more]
A0A6J1DU806.2e-18285.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102352... [more]
Match NameE-valueIdentityDescription
AT1G49780.11.2e-3928.89plant U-box 26 [more]
AT3G19380.12.2e-3828.28plant U-box 25 [more]
AT5G64660.12.9e-3029.73CYS, MET, PRO, and GLY protein 2 [more]
AT3G49810.14.1e-2928.07ARM repeat superfamily protein [more]
AT3G18710.11.0e-2726.70plant U-box 29 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 12..75
e-value: 1.4E-29
score: 114.2
IPR003613U box domainPFAMPF04564U-boxcoord: 9..78
e-value: 1.1E-17
score: 64.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 8..82
score: 30.696344
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..89
e-value: 5.5E-27
score: 95.6
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 182..397
e-value: 7.3E-11
score: 43.2
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 8..390
NoneNo IPR availablePANTHERPTHR22849:SF103OS02G0542800 PROTEINcoord: 8..390
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 13..55
e-value: 2.30644E-26
score: 98.0746
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 4..81
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 231..260
score: 8.627399
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 152..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc02G00460.1Clc02G00460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity