Clc01G09400 (gene) Watermelon (cordophanus) v2

BLAST of Clc01G09400 vs. NCBI nr
Match: XP_038894574.1 (alpha-glucosidase [Benincasa hispida])

HSP 1 Score: 1673.3 bits (4332), Expect = 0.0e+00
Identity = 828/918 (90.20%), Postives = 861/918 (93.79%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSL---LLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPA 60
           M SGSK+MRTPFSQSL   LLFPA LL FLFP  F  L   SL AVGYGYRIRS+HVDPA
Sbjct: 1   MGSGSKAMRTPFSQSLQPPLLFPA-LLFFLFPSFFPNLVAGSLSAVGYGYRIRSSHVDPA 60

Query: 61  GKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSN 120
           GKSLTADL LIRTSPV GPD+  LTLQATFETKDRLRIRITDSTRERWEIPD IIPRRS+
Sbjct: 61  GKSLTADLDLIRTSPVNGPDVPRLTLQATFETKDRLRIRITDSTRERWEIPDQIIPRRSS 120

Query: 121 SRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSE 180
           SRIRSLPENHVGS + SFI DPASDLIF+LH T PFGFSVLRRSSGDVLFDTSPD SDSE
Sbjct: 121 SRIRSLPENHVGSPRTSFISDPASDLIFTLHYTAPFGFSVLRRSSGDVLFDTSPDLSDSE 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNL 240
           TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKL+PDKNKTLTLWNADIGS NLD NL
Sbjct: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLMPDKNKTLTLWNADIGSVNLDVNL 240

Query: 241 YGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAG 300
           YGAHPFYIDVRS S DGKVAAGTTHGVLLLNSNGMDIIYSG R+ YKVIGGIIDLYFFAG
Sbjct: 241 YGAHPFYIDVRSPSGDGKVAAGTTHGVLLLNSNGMDIIYSGHRIMYKVIGGIIDLYFFAG 300

Query: 301 PSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLE 360
           PSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSD+ESVVARYAKAGIPLE
Sbjct: 301 PSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDLESVVARYAKAGIPLE 360

Query: 361 VLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIR 420
           V+WTDIDYMDGYKDFTFDP+NFP +KMKKFVDNLH+NGQKYVLILDPGISTNNTYGTFIR
Sbjct: 361 VMWTDIDYMDGYKDFTFDPVNFPAEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTFIR 420

Query: 421 GTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNE 480
           G + DIFI+YDG+PYLGEVWPG VYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNE
Sbjct: 421 GKRADIFIQYDGVPYLGEVWPGPVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNE 480

Query: 481 IANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540
           I+NFITSSTS FSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES
Sbjct: 481 ISNFITSSTSPFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540

Query: 541 KATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIP 600
           KATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDL  +IPSILNFGLFGIP
Sbjct: 541 KATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLRNTIPSILNFGLFGIP 600

Query: 601 MVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLAL 660
           MVGADICGF GDTTEELC RWIQ+GAFYPF+RDHSDKGSIRQELYLWDTVAASA+KVLAL
Sbjct: 601 MVGADICGFSGDTTEELCGRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLAL 660

Query: 661 RYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAV 720
           RYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTY INSQFLLG GVLVSPV+KEGAV
Sbjct: 661 RYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYGINSQFLLGEGVLVSPVLKEGAV 720

Query: 721 SVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAA 780
           SVDAYFP GNWFSLFNYSELVAVKSGQ+ITLDAPADHINVH+REGNILALH +AMTT+AA
Sbjct: 721 SVDAYFPTGNWFSLFNYSELVAVKSGQRITLDAPADHINVHVREGNILALHGKAMTTRAA 780

Query: 781 RETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVIN 840
           RETAYELLVV+SN QSSFGEVFLDDGEVVEMGA+GGNWSLVRF+SEAVGSKL+VKSQVIN
Sbjct: 781 RETAYELLVVVSNDQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEAVGSKLVVKSQVIN 840

Query: 841 GGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMNAEIS 900
           GGFALSQ LIIDKVTFVGFERPKKM  LGLNISKGV L GNSTIRKTY+YFAK MN EIS
Sbjct: 841 GGFALSQNLIIDKVTFVGFERPKKMVDLGLNISKGVKLNGNSTIRKTYKYFAKVMNVEIS 900

Query: 901 GLSIPIWEEFILEITPIS 916
           GLSIPIWEEF+LE+TPIS
Sbjct: 901 GLSIPIWEEFMLEMTPIS 912

BLAST of Clc01G09400 vs. NCBI nr
Match: XP_008459069.1 (PREDICTED: alpha-glucosidase [Cucumis melo] >KAA0043261.1 alpha-glucosidase [Cucumis melo var. makuwa] >TYK29325.1 alpha-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 801/918 (87.25%), Postives = 853/918 (92.92%), Query Frame = 0

Query: 1   MASGSK---SMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPA 60
           MASGS    ++RTPF+ SLL    +L+LFLF         ASLPAVG+GYRIRS+H+DPA
Sbjct: 1   MASGSSKAITIRTPFAHSLL----ILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPA 60

Query: 61  GKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSN 120
           GKSLTADL LIRTS VYGPDL +LTLQATFE+KDRLR+RITDSTRERWEIPDHIIPR SN
Sbjct: 61  GKSLTADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSN 120

Query: 121 SRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSE 180
           S IRSLPENHV S K SFI DPASDLIF+L+DT PFGFSVLRRSSGDVLFDTSPDFS+SE
Sbjct: 121 SLIRSLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSE 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNL 240
           TF+VFKDQYIQLSS LPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS NLD NL
Sbjct: 181 TFIVFKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNL 240

Query: 241 YGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAG 300
           YGAHPFYIDVRSRSRDGKV AGTTHGVLLLNSNGMDIIYSGDR+TYKVIGGIIDLYFFAG
Sbjct: 241 YGAHPFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAG 300

Query: 301 PSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLE 360
           PSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVS++ESVVARYAKA IPLE
Sbjct: 301 PSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSEVESVVARYAKASIPLE 360

Query: 361 VLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIR 420
           V+WTDIDYMDGYKDFTFDPINFP +KMK FVDNLH+NGQKYV+ILDPGISTNNTYG +IR
Sbjct: 361 VMWTDIDYMDGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIR 420

Query: 421 GTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNE 480
           GTK DIF+K+DG+PYLG+VWPG VYFPDFLHP+SE FWG EIQ+FRDIVPFDGLWIDMNE
Sbjct: 421 GTKADIFMKHDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNE 480

Query: 481 IANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540
           I+NFITSSTS  SNLDNPPYMINNA VRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES
Sbjct: 481 ISNFITSSTSPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540

Query: 541 KATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIP 600
           +ATHASLVKVTGKRPFVLSRSTF GSGKYTAHWTGD GATWND+GY+IPSILNFGLFGIP
Sbjct: 541 RATHASLVKVTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIP 600

Query: 601 MVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLAL 660
           MVG+DICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWD+VAASA+KVLAL
Sbjct: 601 MVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLAL 660

Query: 661 RYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAV 720
           RY+LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEI+SQFLLG GVLVSPV+KEGA 
Sbjct: 661 RYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAF 720

Query: 721 SVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAA 780
           SVDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVH+REGNILALH EAMTT+AA
Sbjct: 721 SVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAA 780

Query: 781 RETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVIN 840
           RETAY+LLVVISNGQ SFGEVFLDDGEV EMG +GGNWS+VRF SE VGSKL+VKSQVIN
Sbjct: 781 RETAYKLLVVISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVIN 840

Query: 841 GGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMNAEIS 900
           GGFALSQKLIIDKVTFVGFERPKKM GLGLNISKGV+L GNS+IRKTYQYFAKFMN EIS
Sbjct: 841 GGFALSQKLIIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEIS 900

Query: 901 GLSIPIWEEFILEITPIS 916
           GLSIPIWEEFILE+TPIS
Sbjct: 901 GLSIPIWEEFILEMTPIS 909

BLAST of Clc01G09400 vs. NCBI nr
Match: XP_011660330.1 (alpha-glucosidase [Cucumis sativus] >KGN66921.1 hypothetical protein Csa_007658 [Cucumis sativus])

HSP 1 Score: 1623.6 bits (4203), Expect = 0.0e+00
Identity = 797/916 (87.01%), Postives = 852/916 (93.01%), Query Frame = 0

Query: 1   MASG-SKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPAGK 60
           MASG SK +RTPF  SL     +L+LFLF  SF  L  ASLPAVG GYRIRS+HVDPAGK
Sbjct: 15  MASGSSKVIRTPFPHSL----PILILFLF-TSFLPLPAASLPAVGLGYRIRSSHVDPAGK 74

Query: 61  SLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSNSR 120
           +LTADL LI TS VYGPDL +LTLQATFE+KDRLR+RITDSTRERWE+P HI+PR S+S 
Sbjct: 75  TLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHHILPRPSSSL 134

Query: 121 IRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSETF 180
           IRSLPENHV S KASFI  PASDLIF+LHDT PFGFSVLRRSSGDVLFDTSP FSDSETF
Sbjct: 135 IRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETF 194

Query: 181 LVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNLYG 240
           LVFKDQYIQLSSSLPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS NLD NLYG
Sbjct: 195 LVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYG 254

Query: 241 AHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPS 300
           AHPFYID+RS S+DGKVAAGTTHGVLLLNSNGMDI+YSGDR+TYKVIGGIIDLYFFAGPS
Sbjct: 255 AHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGIIDLYFFAGPS 314

Query: 301 PISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLEVL 360
           PISV+DQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSDIESVVARYAKA IPLE +
Sbjct: 315 PISVVDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDIESVVARYAKASIPLEAM 374

Query: 361 WTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIRGT 420
           WTDIDYMDGYKDFTFDPINFP  KMK FVDNLH+NGQKYVLILDPGISTNNTYG +IRGT
Sbjct: 375 WTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGT 434

Query: 421 KDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEIA 480
           K DIF+KY+G+PYLG+VWPG VYFPDF HP+SE FWG EIQ+FRDIVPFDGLWIDMNEI+
Sbjct: 435 KADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEIS 494

Query: 481 NFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKA 540
           NFITSSTS  SNLDNPPYMINNA V+RPLNNKTVPASILHFGNLTEYNTHNLYGFLES+A
Sbjct: 495 NFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRA 554

Query: 541 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIPMV 600
           THASLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGY+IPSILNFGLFGIPMV
Sbjct: 555 THASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMV 614

Query: 601 GADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLALRY 660
           G+DICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWD+VAASA+KVLALRY
Sbjct: 615 GSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRY 674

Query: 661 RLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAVSV 720
           +LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKT+EI+SQFLLGGGVLVSPV+KEGA SV
Sbjct: 675 QLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSV 734

Query: 721 DAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAARE 780
           DAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVH+REGNILALH EAMTT+AA+E
Sbjct: 735 DAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQE 794

Query: 781 TAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVINGG 840
           T Y+LLVVISNGQSSFGEVFLDDGEVVEMG +GGNWS+VRF+SE VGSKL+VKSQVINGG
Sbjct: 795 TPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGG 854

Query: 841 FALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMNAEISGL 900
           FALSQKLIIDKVTFVGF+RPKKM  LGLNISKG+NL GNS+IRKTYQYFAKFMN EISGL
Sbjct: 855 FALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGL 914

Query: 901 SIPIWEEFILEITPIS 916
           SIPIWEEFILE+TPI+
Sbjct: 915 SIPIWEEFILEMTPIN 920

BLAST of Clc01G09400 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1590.9 bits (4118), Expect = 0.0e+00
Identity = 780/925 (84.32%), Postives = 843/925 (91.14%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLP----------AVGYGYRIR 60
           MA+GSKS++    +  LL   +L LF    S F   +ASLP          AVGYGYR+R
Sbjct: 22  MATGSKSLQAFLRRRRLLSAVVLFLF----SCFFRLIASLPFAAGEQRENSAVGYGYRVR 81

Query: 61  STHVDPAGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDH 120
           S  VDP GKSLTADL LI  S VYGPD+  L+LQA+FETKDRLR+RITDS RERWEIPD 
Sbjct: 82  SGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEIPDE 141

Query: 121 IIPRRSNSRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTS 180
           IIPRRSNSRIRSLPE  VGS + S I DPASDLIFSLHDT PFGFSV RRSSGDVLFDTS
Sbjct: 142 IIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTS 201

Query: 181 PDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGS 240
           P+FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFK+VPDK+KTLTLW+ADIGS
Sbjct: 202 PNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGS 261

Query: 241 RNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGII 300
            N D NLYGAHPFYIDVRS SRDGKVAAGTTHGVLLLNSNGMDIIYSGDR++YK IGGII
Sbjct: 262 VNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGII 321

Query: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYA 360
           DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSDIE+VVARYA
Sbjct: 322 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDIETVVARYA 381

Query: 361 KAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNN 420
           KAGIPLEV+WTDIDYMDGYKDFTFDPINFPV+KMK FVD+LH+NGQKYVLI+DPGISTN 
Sbjct: 382 KAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNK 441

Query: 421 TYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDG 480
           TYGT+IRG + DIFIKYDG+PYLGEVWPG VYFPDFLHPNSE FWGGEI+LFRDIVPFDG
Sbjct: 442 TYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDG 501

Query: 481 LWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHN 540
           LWIDMNE++NFITSSTS FSNLDNPPY I+NAGV+RP+NN+TVPAS LHFGNLTEYNTHN
Sbjct: 502 LWIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHN 561

Query: 541 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILN 600
           LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGY+IPSILN
Sbjct: 562 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILN 621

Query: 601 FGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAAS 660
           FGLFGIPMVGADICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWDTVAAS
Sbjct: 622 FGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAAS 681

Query: 661 AKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSP 720
           A+KVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVLVSP
Sbjct: 682 ARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSP 741

Query: 721 VVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHRE 780
           V+KEGA+SVDAYFPAGNWFSLFNYSE VAVKSGQQITLDAPADHINVH+REGNILALH E
Sbjct: 742 VLKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVHVREGNILALHGE 801

Query: 781 AMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLL 840
           A TT+AAR+TA++LLVV+SNGQSS GEVFLDDGEVVEMG +GGNWSLVRF+SEAVGSKLL
Sbjct: 802 ATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLL 861

Query: 841 VKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAK 900
           +KSQVINGGFALSQK+IIDKVTFVGFERPKKMGGLGL+ISKG NL GNS IRKTY+Y AK
Sbjct: 862 IKSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAK 921

Query: 901 FMNAEISGLSIPIWEEFILEITPIS 916
           F+N EISGLSIPI EEF++E++P++
Sbjct: 922 FVNVEISGLSIPICEEFVMELSPLN 937

BLAST of Clc01G09400 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1588.5 bits (4112), Expect = 0.0e+00
Identity = 780/925 (84.32%), Postives = 839/925 (90.70%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLP----------AVGYGYRIR 60
           MA+GSKS++    +  LL   +L LF     FF L  ASLP          AVGYGYR+R
Sbjct: 22  MATGSKSLQAFLRRRRLLSAVVLFLF---TCFFRL-TASLPFAAGEQRENSAVGYGYRVR 81

Query: 61  STHVDPAGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDH 120
           S  VDP GKSLTADL LI  S VYGPD+  L++QA+FETKDRLR+RITDS RERWE+PD 
Sbjct: 82  SGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDE 141

Query: 121 IIPRRSNSRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTS 180
           IIPRRSNSRIRSLPE  VGS + S I DPASDLIFSLHDT PFGFSV RRSSGDVLFDTS
Sbjct: 142 IIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTS 201

Query: 181 PDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGS 240
           P+FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFKLVPDK+KTLTLWNADIGS
Sbjct: 202 PNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGS 261

Query: 241 RNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGII 300
            N D NLYGAHPFYIDVRS SRDGKVAAGTTHGVLLLNSNGMDIIYSGDR++YK IGGII
Sbjct: 262 VNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGII 321

Query: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYA 360
           DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSDIE+VVARYA
Sbjct: 322 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDIETVVARYA 381

Query: 361 KAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNN 420
           KAGIPLEV+WTDIDYMDGYKDFTFDPINFPV+KMK FVDNLH+NGQKYVLILDPGISTN 
Sbjct: 382 KAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNK 441

Query: 421 TYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDG 480
           TYGT+IRG + DIFIKYDG+PYLGEVWPG VYFPDFLHPNSE FWG EI+LFRDIVPFDG
Sbjct: 442 TYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDG 501

Query: 481 LWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHN 540
           LWIDMNEI+NFITSSTSSFSNLDNPPY INNAGV+RP+NN+TVPAS LHFGNLTEYNTHN
Sbjct: 502 LWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHN 561

Query: 541 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILN 600
           LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGY+IPSILN
Sbjct: 562 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILN 621

Query: 601 FGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAAS 660
           FGLFGIPMVGADICGF  DTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWDTVAAS
Sbjct: 622 FGLFGIPMVGADICGFSRDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAAS 681

Query: 661 AKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSP 720
           A+KVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVL+SP
Sbjct: 682 ARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISP 741

Query: 721 VVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHRE 780
           V+KEGA+SVDAYFPAGNWFSLFNYSE VAV SGQQITLDAPADHINVH+REGNILALH E
Sbjct: 742 VLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGE 801

Query: 781 AMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLL 840
           A TT+AAR+TA++LLVV+SNGQSS GEVFLDDGEVVEMG +GGNWSLVRF+SEAVGSKLL
Sbjct: 802 ATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLL 861

Query: 841 VKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAK 900
           +KSQVINGGFALSQK+IIDKVT VGFERPK MGGLGL+ISKG NL GNS IRKTY+Y AK
Sbjct: 862 IKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAK 921

Query: 901 FMNAEISGLSIPIWEEFILEITPIS 916
           F+N EISGLSI IWEEF++E++P++
Sbjct: 922 FVNVEISGLSISIWEEFVMELSPLN 937

BLAST of Clc01G09400 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 523/877 (59.64%), Postives = 664/877 (75.71%), Query Frame = 0

Query: 43  VGYGYRIRSTHVDP-AGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDST 102
           +GYGY+++S  VD    +SLTA   L++ S VYGPD+  L++ A+ E+ DRLR+RITD+ 
Sbjct: 36  IGYGYKVKSVKVDSGTRRSLTALPQLVKNSSVYGPDIQLLSITASLESNDRLRVRITDAK 95

Query: 103 RERWEIPDHII---------PRRSNSRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTP 162
             RWEIPD+I+         P   +S  R+L  +   + +   +  P SDL FSL +TTP
Sbjct: 96  HRRWEIPDNILHRHQPPPPPPHSLSSLYRTLLSSPTTNRRKILLSHPNSDLTFSLINTTP 155

Query: 163 FGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFK 222
           FGF++ R+S+ DVLFD +PD ++  TFL+F DQY+ L+SSLP  R+ I+G+GE ++ +F+
Sbjct: 156 FGFTISRKSTHDVLFDATPDPTNPNTFLIFIDQYLHLTSSLPGTRAHIYGLGEHSKPTFQ 215

Query: 223 LVPDKNKTLTLWNADIGSRNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGM 282
           L    N+TLT+  ADI S N D NLYG+HPFY+DVRS        AG+THGVLLLNSNGM
Sbjct: 216 LA--HNQTLTMRAADIPSSNPDVNLYGSHPFYMDVRS-----SPVAGSTHGVLLLNSNGM 275

Query: 283 DIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQC 342
           D+ Y+G+R+TYKVIGGIIDLYFFAGPSP  V++Q+T +IGRPAP+PYW+FG     F QC
Sbjct: 276 DVEYTGNRITYKVIGGIIDLYFFAGPSPGQVVEQFTRVIGRPAPMPYWAFG-----FQQC 335

Query: 343 RYGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLH 402
           RYGY +V +++SVVA YAKA IPLEV+WTDIDYMD YKDFT DP+NFP+DKMKKFV+NLH
Sbjct: 336 RYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYKDFTLDPVNFPLDKMKKFVNNLH 395

Query: 403 ENGQKYVLILDPGISTNNTYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSE 462
           +NGQKYV+ILDPGISTN TY T+IRG K D+F+K +G PYLG VWPG VYFPDFL P++ 
Sbjct: 396 KNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKRNGKPYLGSVWPGPVYFPDFLKPSAL 455

Query: 463 NFWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKT 522
            FW  EI+ F +++P DGLWIDMNEI+NFI+S     S LDNPPY INN+GV  P+ NKT
Sbjct: 456 TFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPIPGSTLDNPPYKINNSGVMLPIINKT 515

Query: 523 VPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTG 582
           +P + +H+G++ EYN HNL+G+LE++ T A+L+K+T KRPFVLSRSTF GSGKYTAHWTG
Sbjct: 516 IPPTAMHYGDIPEYNVHNLFGYLEARVTRAALIKLTEKRPFVLSRSTFSGSGKYTAHWTG 575

Query: 583 DNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHS 642
           DN ATWNDL YSIPS+L+FGLFGIPMVGADICGF G+TTEELC+RWIQ+GAFYPFSRDHS
Sbjct: 576 DNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFLGNTTEELCRRWIQLGAFYPFSRDHS 635

Query: 643 DKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIK 702
             G+  QELY W++VAASA+KVL LRY LLPYFYTLMYEA   G PIARPLFFSFP DIK
Sbjct: 636 SLGTTYQELYRWESVAASARKVLGLRYTLLPYFYTLMYEAQLNGIPIARPLFFSFPDDIK 695

Query: 703 TYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPA 762
           TY I+SQFLLG GV+VSPV+K G VSV AYFP GNWF LF+Y+  V   +G+ +TL AP 
Sbjct: 696 TYGISSQFLLGKGVMVSPVLKPGVVSVTAYFPRGNWFDLFDYTRSVTASTGRYVTLSAPP 755

Query: 763 DHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADG 822
           DHINVHI+EGNILA+  +AMTT+AAR+T + LLVV+S+  +SFGE+FLDDG  V MG + 
Sbjct: 756 DHINVHIQEGNILAMQGKAMTTQAARKTPFHLLVVMSDCGASFGELFLDDGVEVTMGVNR 815

Query: 823 GNWSLVRFFSEAVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKG 882
           G W+ V+F + +     ++ S V++G FA+SQK +IDKVT +G  +  K+ G  +     
Sbjct: 816 GKWTFVKFIAASAKQTCIITSDVVSGEFAVSQKWVIDKVTILGLRKGTKINGYTVRTGAV 875

Query: 883 VNLKGNSTIRKTYQYFAKFMNAEISGLSIPIWEEFIL 910
                 S ++ T     +F+ AEISGL++ +  EF L
Sbjct: 876 TRKGDKSKLKSTPDRKGEFIVAEISGLNLLLGREFKL 900

BLAST of Clc01G09400 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 6.3e-304
Identity = 507/883 (57.42%), Postives = 665/883 (75.31%), Query Frame = 0

Query: 42  AVGYGYRIRSTHVD-PAGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDS 101
           A+GYGY++++  VD   GKSLTA L LIR SPVYGPD+  L+  A+FE  D LRIR TD+
Sbjct: 40  AIGYGYQVKNAKVDNSTGKSLTALLQLIRNSPVYGPDIHFLSFTASFEEDDTLRIRFTDA 99

Query: 102 TRERWEIPDHIIPR----RSNSRIRSL-------PENHVGSTKASFIFDPASDLIFSLHD 161
              RWEIP+ ++PR     S   + SL       P+N   +T  S    P SDL F+L  
Sbjct: 100 NNRRWEIPNEVLPRPPPPPSPPPLSSLQHLPKPIPQNQPTTTVLS---HPHSDLAFTLFH 159

Query: 162 TTPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRE 221
           TTPFGF++ R+S+ DVLFD +P  S+  TFL++KDQY+QLSSSLP  ++ ++G+GE T+ 
Sbjct: 160 TTPFGFTIYRKSTHDVLFDATPIPSNPTTFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKP 219

Query: 222 SFKLVPDKNKTLTLWNADIGSRNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNS 281
           +F+L    N+ LTLWNADI S N D NLYG+HPFY+DVRS         G+THGV LLNS
Sbjct: 220 TFQLA--HNQILTLWNADIASFNRDLNLYGSHPFYMDVRS-----SPMVGSTHGVFLLNS 279

Query: 282 NGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCF 341
           NGMD+ Y+GDR+TYKVIGGIIDLY FAG +P  V+DQYT+LIGRPAP+PYW+FG     F
Sbjct: 280 NGMDVEYTGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFG-----F 339

Query: 342 HQCRYGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVD 401
           HQCR+GY++V++IE+VV +YA+A IPLEV+WTDIDYMD +KDFT DP++FP+DKM++FV 
Sbjct: 340 HQCRWGYRDVNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVT 399

Query: 402 NLHENGQKYVLILDPGISTNNTYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHP 461
            LH NGQ+YV ILDPGI+TN +YGTFIRG + ++FIK +G PYLG VWPG VY+PDFL P
Sbjct: 400 KLHRNGQRYVPILDPGINTNKSYGTFIRGMQSNVFIKRNGNPYLGSVWPGPVYYPDFLDP 459

Query: 462 NSENFWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLN 521
            + +FW  EI+ FRDI+P DG+WIDMNE +NFITS+ +  S LDNPPY INN+G R P+N
Sbjct: 460 AARSFWVDEIKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPIN 519

Query: 522 NKTVPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAH 581
           +KT+PA+ +H+GN+TEYN HNLYGFLES+AT  +LV+   + PF+LSRSTF GSGKYTAH
Sbjct: 520 SKTIPATAMHYGNVTEYNAHNLYGFLESQATREALVRPATRGPFLLSRSTFAGSGKYTAH 579

Query: 582 WTGDNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSR 641
           WTGDN A W+DL YSIP++LNFGLFG+PM+GADICGF   TTEELC RWIQ+GAFYPFSR
Sbjct: 580 WTGDNAARWDDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSR 639

Query: 642 DHSDKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQ 701
           DHS + +  QELYLW++VAASA+ VL LRY LLPY+YTLMY+A+ +G+PIARPL F+FP 
Sbjct: 640 DHSARDTTHQELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPD 699

Query: 702 DIKTYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLD 761
           D+ TY I+SQFL+G G++VSPV++ G+  V+AY P GNW SL NY+  V+V +G  ++L 
Sbjct: 700 DVATYGISSQFLIGRGIMVSPVLQPGSSIVNAYSPRGNWVSLSNYTSSVSVSAGTYVSLS 759

Query: 762 APADHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMG 821
           AP DHINVHI EGNI+A+  EAMTT+AAR T + LLVV+S+  +S GE+FLD+G  +++G
Sbjct: 760 APPDHINVHIHEGNIVAMQGEAMTTQAARSTPFHLLVVMSDHVASTGELFLDNGIEMDIG 819

Query: 822 ADGGNWSLVRFFSEAVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNI 881
             GG W+LVRFF+E+  + L + S+V+N G+A+SQ+ ++DK+T +G +R  K+    +  
Sbjct: 820 GPGGKWTLVRFFAESGINNLTISSEVVNRGYAMSQRWVMDKITILGLKRRVKIKEYTVQK 879

Query: 882 SKG-VNLKGNSTIRKTYQYFAKFMNAEISGLSIPIWEEFILEI 912
             G + +KG      ++     F++  IS L   + + F LE+
Sbjct: 880 DAGAIKVKGLGRRTSSHNQGGFFVSV-ISDLRQLVGQAFKLEL 906

BLAST of Clc01G09400 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 4.0e-290
Identity = 492/889 (55.34%), Postives = 644/889 (72.44%), Query Frame = 0

Query: 35  LHVASLPAVGYGYRIRSTHVDPAGKSLTADLHLI----RTSPVYGPDLLSLTLQATFETK 94
           L +A+   V  GY + S  V  +   L A L L       +P  GPD+  L+L A+ ET 
Sbjct: 23  LFLAAPWGVDCGYNVAS--VAGSKNRLRARLELAGGGGGAAPELGPDVRRLSLTASLETD 82

Query: 95  DRLRIRITDSTRERWEIPDHIIPRRSNSRIRSLPENHVGSTK---ASFIFDPASDLIFSL 154
            RL +RITD+   RWE+P  +IPR S       P++ + +T+      +    SDL F++
Sbjct: 83  SRLHVRITDADHPRWEVPQDVIPRPS-------PDSFLAATRPGGGRVLSTATSDLTFAI 142

Query: 155 HDTTPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSL-PKDRSSIFGIGEQ 214
           H T+PF F+V RRS+GDVLFDT+P+       LVFKD+Y++L+SSL P  R+S++G+GEQ
Sbjct: 143 H-TSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRYLELTSSLPPPGRASLYGLGEQ 202

Query: 215 TRESFKLVPDKNKTLTLWNADIGSRNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLL 274
           T+ +F+L   +N T TLWN+DI + N+D NLYG+HPFY+DVRS    G    G  HGVLL
Sbjct: 203 TKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDVRSGGGGG---GGAAHGVLL 262

Query: 275 LNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYF 334
           LNSNGMD+IY G  VTYKVIGG++D YFFAGPSP++V+DQYT+LIGRPAP+PYWSFG   
Sbjct: 263 LNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQYTQLIGRPAPMPYWSFG--- 322

Query: 335 YCFHQCRYGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKK 394
             FHQCRYGYKNV+D+E VVA YAKA IPLEV+WTDIDYMD YKDFT DP+NFP D+M+ 
Sbjct: 323 --FHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDFTLDPVNFPADRMRP 382

Query: 395 FVDNLHENGQKYVLILDPGISTNNTYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDF 454
           FVD LH NGQK+V+I+DPGI+ N TYGTF+RG K DIF+K++G  YLG VWPG VYFPDF
Sbjct: 383 FVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNYLGVVWPGNVYFPDF 442

Query: 455 LHPNSENFWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRR 514
           L+P +  FW  EI  FR  +P DGLW+DMNEI+NF+       + +D+PPY INN+GVRR
Sbjct: 443 LNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLNAIDDPPYRINNSGVRR 502

Query: 515 PLNNKTVPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKY 574
           P+NNKTVPAS +H+G + EY+ HNL+GFLE++ATH +L++ TG+RPFVLSRSTFVGSG+Y
Sbjct: 503 PINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRPFVLSRSTFVGSGRY 562

Query: 575 TAHWTGDNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYP 634
           TAHWTGDN ATW DL YSI ++L+FGLFGIPM+GADICGF G+TTEELC RWIQ+GAFYP
Sbjct: 563 TAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTEELCSRWIQLGAFYP 622

Query: 635 FSRDHSDKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFS 694
           FSRDHS  G++R+ELYLW++VA SA+K L LRYRLLPY YTLMYEAH  G PIARPLFFS
Sbjct: 623 FSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEAHTTGAPIARPLFFS 682

Query: 695 FPQDIKTYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQI 754
           +P D++TY I+ QFLLG GVLVSPV++ GA +V AYFPAG WFSL+++S  VA K+G+++
Sbjct: 683 YPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLYDFSLAVATKTGKRV 742

Query: 755 TLDAPADHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVV 814
           TL APAD +NVH+  GNIL L + A+T+   R++   LLV +++  ++ G++FLDDGE  
Sbjct: 743 TLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDGTATGDLFLDDGESP 802

Query: 815 EMGADGGNWSLVRF--FSEAVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGG 874
           EM      WS ++F   +E+ G  + V+S V++  +A S+ + I KV  +G        G
Sbjct: 803 EMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAIGKVVLMGLRSAAPPKG 862

Query: 875 LGLNISKGVNLKGNSTIRKTYQYFAK--FMNAEISGLSIPIWEEFILEI 912
             +  + GV +  ++ +        K     A +SGL++ + +EF L++
Sbjct: 863 FAV-YANGVQVNASTAVGGAAGSPEKGALGVAHVSGLTLVVGQEFDLKV 881

BLAST of Clc01G09400 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 7.1e-271
Identity = 459/842 (54.51%), Postives = 601/842 (71.38%), Query Frame = 0

Query: 77  DLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRS-NSRIRSLPENHVGSTKASF 136
           D+  L + A+ ET  RLR+RITD+   RWE+P  IIPR +    +   P       +   
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPASSAPLQGRV 118

Query: 137 IFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPK 196
           +    SDL+ ++H  +PF F+V RRS+GD LFDT+P        LVF+D+Y++++S+LP 
Sbjct: 119 LSPAGSDLVLTVH-ASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSALPA 178

Query: 197 DRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNLYGAHPFYIDVRSRSRDGK 256
            R+S++G+GE T+ SF+L    N + TLWNADIG+  +D NLYG+HPFY+DVR       
Sbjct: 179 GRASLYGLGEHTKSSFRL--RHNDSFTLWNADIGASYVDVNLYGSHPFYMDVR------- 238

Query: 257 VAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 316
            A GT HGVLLL+SNGMD++Y G  VTYKVIGG++D YFFAGP+P++V+DQYT+LI RPA
Sbjct: 239 -APGTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPA 298

Query: 317 PVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFD 376
           P+PYWSFG     FHQCRYGY NVSD+E VVARYAKA IPLEV+WTDIDYMDG+KDFT D
Sbjct: 299 PMPYWSFG-----FHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLD 358

Query: 377 PINFPVDKMKKFVDNLHENGQKYVLILDPGIST---NNTYGTFIRGTKDDIFIKYDGIPY 436
            +NF   +++ FVD LH N QKYVLILDPGI     + TYGTF+RG + DIF+K +G  +
Sbjct: 359 RVNFTAAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNF 418

Query: 437 LGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNL 496
           +G VWPG VYFPDF+HP +  FW  EI LFR  +P DGLWIDMNEI+NF   +    + L
Sbjct: 419 VGNVWPGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNAL 478

Query: 497 DNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRP 556
           D+PPY INN G  RP+NNKTV    +H+G +TEY  HNL+G LE++AT   +++ TG+RP
Sbjct: 479 DDPPYRINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRP 538

Query: 557 FVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTE 616
           FVLSRSTFVGSG+YTA+WTGDN ATW DL YSI ++L+FGLFG+PM+GADICGF G+TTE
Sbjct: 539 FVLSRSTFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTE 598

Query: 617 ELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEA 676
           ELC RWIQ+GAFYPFSRDHS   ++R+ELYLW +VAAS +K L LRY+LLPYFYTLMYEA
Sbjct: 599 ELCGRWIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEA 658

Query: 677 HKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLF 736
           H  G PIARPLFFS+P D+ TY ++ QFLLG GVLVSPV++ G  +VDAYFPAG W+ L+
Sbjct: 659 HMTGAPIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLY 718

Query: 737 NYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQ 796
           +YS  VA ++G+ + L APAD +NVH+  G IL L + A+TT  AR TA+ LLV ++   
Sbjct: 719 DYSLAVATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDG 778

Query: 797 SSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSK--LLVKSQVINGGFALSQKLIIDK 856
           ++ G +FLDDG+  E G    +WS+VRF  +   +K  + VKS+V++  +A S+ L+I K
Sbjct: 779 TASGYLFLDDGDSPEYGR-RSDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISK 838

Query: 857 VTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMN-AEISGLSIPIWEEFIL 912
           V  +G   P     L ++++    ++ +S+    YQ        A I GLS+ + EEF L
Sbjct: 839 VVLMGHRSPAAPKKLTVHVN-SAEVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFEL 873

BLAST of Clc01G09400 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 2.4e-234
Identity = 424/944 (44.92%), Postives = 601/944 (63.67%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPAGKS 60
           MAS S S+   FS SLL    L L+  F P+       S   +G GYR+ S    P G  
Sbjct: 1   MASSSSSL--AFSLSLL----LALILCFSPT------QSYKTIGKGYRLVSIEESPDG-G 60

Query: 61  LTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRI 120
               L + + + +YG D+ +L L    ET  RLR+ ITD+ ++RWE+P +++PR    ++
Sbjct: 61  FIGYLQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQV 120

Query: 121 RSLPENHVGSTKASFIF---DPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSE 180
             +    +G ++ S I       S+LIFS + T PF F+V RRS+ + LF+T+       
Sbjct: 121 GKV----IGKSRKSPITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTT------- 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR-ESFKLVPDKNKTLTLWNADIGSRNLDTN 240
           + LVFKDQY+++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ + NL+T+
Sbjct: 181 SSLVFKDQYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTD 240

Query: 241 LYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFA 300
           LYG+HP Y+D+R+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF A
Sbjct: 241 LYGSHPMYMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIA 300

Query: 301 GPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPL 360
           GPSP++V+DQYT+LIGRPAP+PYWS G     FHQCR+GY N+S +E VV  Y KA IPL
Sbjct: 301 GPSPLNVVDQYTQLIGRPAPMPYWSLG-----FHQCRWGYHNLSVVEDVVDNYKKAKIPL 360

Query: 361 EVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFI 420
           +V+W D D+MDG+KDFT +P+ +P  K+  F+D +H+ G KY++I DPGI  N +YGTF 
Sbjct: 361 DVIWNDDDHMDGHKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQ 420

Query: 421 RGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMN 480
           R    D+FIKY+G P+L +VWPG VYFPDFL+P + ++WG EI+ F D+VP DGLWIDMN
Sbjct: 421 RAMAADVFIKYEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMN 480

Query: 481 EIANF------------------------ITSSTSSFSNLDNPPYMINNAGVRRPLNNKT 540
           E++NF                        +     + +  D+PPY IN  GV  P+  KT
Sbjct: 481 EVSNFCSGLCTIPEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKT 540

Query: 541 VPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTG 600
           +  S  H+  + EY+ H++YGF E+ ATH  L+ V GKRPF+LSRSTFVGSG+Y AHWTG
Sbjct: 541 IATSATHYNGVREYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTG 600

Query: 601 DNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHS 660
           DN  TW  L  SI ++LNFG+FG+PMVG+DICGF+   TEELC RWI+VGAFYPFSRDH+
Sbjct: 601 DNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHA 660

Query: 661 DKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIK 720
           +  S RQELY WDTVA SA+  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  +
Sbjct: 661 NYYSPRQELYQWDTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTE 720

Query: 721 TYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPA 780
            Y  + QFLLG   ++SPV+++G   V+A FP G+W+ +F+ ++ V  K+G+++TL AP 
Sbjct: 721 CYGNSRQFLLGSSFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPL 780

Query: 781 DHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQS---SFGEVFLDDGEVVEMG 840
           + +NVH+ +  IL   +  + +K AR T + L++    G S   + G+++LD+ E+ EM 
Sbjct: 781 NFVNVHLYQNTILPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMK 840

Query: 841 ADGGNWSLVRFFSEAVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNI 900
              G  + V F++      + + SQV  G FALS+  +I+KV+ +G     ++  + +N 
Sbjct: 841 LGNGQSTYVDFYASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQING 900

Query: 901 SKGVNLKGNSTIRKTY------QYFAKFMNAEISGLSIPIWEEF 908
           S        S+   TY      +   K +  E+ GL + + ++F
Sbjct: 901 SPMTKKIEVSSKEHTYVIGLEDEEENKSVMVEVRGLEMLVGKDF 906

BLAST of Clc01G09400 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 801/918 (87.25%), Postives = 853/918 (92.92%), Query Frame = 0

Query: 1   MASGSK---SMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPA 60
           MASGS    ++RTPF+ SLL    +L+LFLF         ASLPAVG+GYRIRS+H+DPA
Sbjct: 1   MASGSSKAITIRTPFAHSLL----ILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPA 60

Query: 61  GKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSN 120
           GKSLTADL LIRTS VYGPDL +LTLQATFE+KDRLR+RITDSTRERWEIPDHIIPR SN
Sbjct: 61  GKSLTADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSN 120

Query: 121 SRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSE 180
           S IRSLPENHV S K SFI DPASDLIF+L+DT PFGFSVLRRSSGDVLFDTSPDFS+SE
Sbjct: 121 SLIRSLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSE 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNL 240
           TF+VFKDQYIQLSS LPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS NLD NL
Sbjct: 181 TFIVFKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNL 240

Query: 241 YGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAG 300
           YGAHPFYIDVRSRSRDGKV AGTTHGVLLLNSNGMDIIYSGDR+TYKVIGGIIDLYFFAG
Sbjct: 241 YGAHPFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAG 300

Query: 301 PSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLE 360
           PSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVS++ESVVARYAKA IPLE
Sbjct: 301 PSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSEVESVVARYAKASIPLE 360

Query: 361 VLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIR 420
           V+WTDIDYMDGYKDFTFDPINFP +KMK FVDNLH+NGQKYV+ILDPGISTNNTYG +IR
Sbjct: 361 VMWTDIDYMDGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIR 420

Query: 421 GTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNE 480
           GTK DIF+K+DG+PYLG+VWPG VYFPDFLHP+SE FWG EIQ+FRDIVPFDGLWIDMNE
Sbjct: 421 GTKADIFMKHDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNE 480

Query: 481 IANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540
           I+NFITSSTS  SNLDNPPYMINNA VRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES
Sbjct: 481 ISNFITSSTSPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540

Query: 541 KATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIP 600
           +ATHASLVKVTGKRPFVLSRSTF GSGKYTAHWTGD GATWND+GY+IPSILNFGLFGIP
Sbjct: 541 RATHASLVKVTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIP 600

Query: 601 MVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLAL 660
           MVG+DICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWD+VAASA+KVLAL
Sbjct: 601 MVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLAL 660

Query: 661 RYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAV 720
           RY+LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEI+SQFLLG GVLVSPV+KEGA 
Sbjct: 661 RYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAF 720

Query: 721 SVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAA 780
           SVDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVH+REGNILALH EAMTT+AA
Sbjct: 721 SVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAA 780

Query: 781 RETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVIN 840
           RETAY+LLVVISNGQ SFGEVFLDDGEV EMG +GGNWS+VRF SE VGSKL+VKSQVIN
Sbjct: 781 RETAYKLLVVISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVIN 840

Query: 841 GGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMNAEIS 900
           GGFALSQKLIIDKVTFVGFERPKKM GLGLNISKGV+L GNS+IRKTYQYFAKFMN EIS
Sbjct: 841 GGFALSQKLIIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEIS 900

Query: 901 GLSIPIWEEFILEITPIS 916
           GLSIPIWEEFILE+TPIS
Sbjct: 901 GLSIPIWEEFILEMTPIS 909

BLAST of Clc01G09400 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 801/918 (87.25%), Postives = 853/918 (92.92%), Query Frame = 0

Query: 1   MASGSK---SMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPA 60
           MASGS    ++RTPF+ SLL    +L+LFLF         ASLPAVG+GYRIRS+H+DPA
Sbjct: 1   MASGSSKAITIRTPFAHSLL----ILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPA 60

Query: 61  GKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSN 120
           GKSLTADL LIRTS VYGPDL +LTLQATFE+KDRLR+RITDSTRERWEIPDHIIPR SN
Sbjct: 61  GKSLTADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSN 120

Query: 121 SRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSE 180
           S IRSLPENHV S K SFI DPASDLIF+L+DT PFGFSVLRRSSGDVLFDTSPDFS+SE
Sbjct: 121 SLIRSLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSE 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNL 240
           TF+VFKDQYIQLSS LPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS NLD NL
Sbjct: 181 TFIVFKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNL 240

Query: 241 YGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAG 300
           YGAHPFYIDVRSRSRDGKV AGTTHGVLLLNSNGMDIIYSGDR+TYKVIGGIIDLYFFAG
Sbjct: 241 YGAHPFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAG 300

Query: 301 PSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLE 360
           PSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVS++ESVVARYAKA IPLE
Sbjct: 301 PSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSEVESVVARYAKASIPLE 360

Query: 361 VLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIR 420
           V+WTDIDYMDGYKDFTFDPINFP +KMK FVDNLH+NGQKYV+ILDPGISTNNTYG +IR
Sbjct: 361 VMWTDIDYMDGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIR 420

Query: 421 GTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNE 480
           GTK DIF+K+DG+PYLG+VWPG VYFPDFLHP+SE FWG EIQ+FRDIVPFDGLWIDMNE
Sbjct: 421 GTKADIFMKHDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNE 480

Query: 481 IANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540
           I+NFITSSTS  SNLDNPPYMINNA VRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES
Sbjct: 481 ISNFITSSTSPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLES 540

Query: 541 KATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIP 600
           +ATHASLVKVTGKRPFVLSRSTF GSGKYTAHWTGD GATWND+GY+IPSILNFGLFGIP
Sbjct: 541 RATHASLVKVTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIP 600

Query: 601 MVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLAL 660
           MVG+DICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWD+VAASA+KVLAL
Sbjct: 601 MVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLAL 660

Query: 661 RYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAV 720
           RY+LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEI+SQFLLG GVLVSPV+KEGA 
Sbjct: 661 RYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAF 720

Query: 721 SVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAA 780
           SVDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVH+REGNILALH EAMTT+AA
Sbjct: 721 SVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAA 780

Query: 781 RETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVIN 840
           RETAY+LLVVISNGQ SFGEVFLDDGEV EMG +GGNWS+VRF SE VGSKL+VKSQVIN
Sbjct: 781 RETAYKLLVVISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVIN 840

Query: 841 GGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMNAEIS 900
           GGFALSQKLIIDKVTFVGFERPKKM GLGLNISKGV+L GNS+IRKTYQYFAKFMN EIS
Sbjct: 841 GGFALSQKLIIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEIS 900

Query: 901 GLSIPIWEEFILEITPIS 916
           GLSIPIWEEFILE+TPIS
Sbjct: 901 GLSIPIWEEFILEMTPIS 909

BLAST of Clc01G09400 vs. ExPASy TrEMBL
Match: A0A0A0M3Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G716250 PE=3 SV=1)

HSP 1 Score: 1623.6 bits (4203), Expect = 0.0e+00
Identity = 797/916 (87.01%), Postives = 852/916 (93.01%), Query Frame = 0

Query: 1   MASG-SKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPAGK 60
           MASG SK +RTPF  SL     +L+LFLF  SF  L  ASLPAVG GYRIRS+HVDPAGK
Sbjct: 15  MASGSSKVIRTPFPHSL----PILILFLF-TSFLPLPAASLPAVGLGYRIRSSHVDPAGK 74

Query: 61  SLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSNSR 120
           +LTADL LI TS VYGPDL +LTLQATFE+KDRLR+RITDSTRERWE+P HI+PR S+S 
Sbjct: 75  TLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHHILPRPSSSL 134

Query: 121 IRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSETF 180
           IRSLPENHV S KASFI  PASDLIF+LHDT PFGFSVLRRSSGDVLFDTSP FSDSETF
Sbjct: 135 IRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETF 194

Query: 181 LVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNLYG 240
           LVFKDQYIQLSSSLPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS NLD NLYG
Sbjct: 195 LVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYG 254

Query: 241 AHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPS 300
           AHPFYID+RS S+DGKVAAGTTHGVLLLNSNGMDI+YSGDR+TYKVIGGIIDLYFFAGPS
Sbjct: 255 AHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGIIDLYFFAGPS 314

Query: 301 PISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLEVL 360
           PISV+DQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSDIESVVARYAKA IPLE +
Sbjct: 315 PISVVDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDIESVVARYAKASIPLEAM 374

Query: 361 WTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIRGT 420
           WTDIDYMDGYKDFTFDPINFP  KMK FVDNLH+NGQKYVLILDPGISTNNTYG +IRGT
Sbjct: 375 WTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGT 434

Query: 421 KDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEIA 480
           K DIF+KY+G+PYLG+VWPG VYFPDF HP+SE FWG EIQ+FRDIVPFDGLWIDMNEI+
Sbjct: 435 KADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEIS 494

Query: 481 NFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKA 540
           NFITSSTS  SNLDNPPYMINNA V+RPLNNKTVPASILHFGNLTEYNTHNLYGFLES+A
Sbjct: 495 NFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRA 554

Query: 541 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIPMV 600
           THASLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGY+IPSILNFGLFGIPMV
Sbjct: 555 THASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMV 614

Query: 601 GADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLALRY 660
           G+DICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWD+VAASA+KVLALRY
Sbjct: 615 GSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRY 674

Query: 661 RLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAVSV 720
           +LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKT+EI+SQFLLGGGVLVSPV+KEGA SV
Sbjct: 675 QLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSV 734

Query: 721 DAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAARE 780
           DAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVH+REGNILALH EAMTT+AA+E
Sbjct: 735 DAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQE 794

Query: 781 TAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVINGG 840
           T Y+LLVVISNGQSSFGEVFLDDGEVVEMG +GGNWS+VRF+SE VGSKL+VKSQVINGG
Sbjct: 795 TPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGG 854

Query: 841 FALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAKFMNAEISGL 900
           FALSQKLIIDKVTFVGF+RPKKM  LGLNISKG+NL GNS+IRKTYQYFAKFMN EISGL
Sbjct: 855 FALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGL 914

Query: 901 SIPIWEEFILEITPIS 916
           SIPIWEEFILE+TPI+
Sbjct: 915 SIPIWEEFILEMTPIN 920

BLAST of Clc01G09400 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1590.9 bits (4118), Expect = 0.0e+00
Identity = 780/925 (84.32%), Postives = 843/925 (91.14%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLP----------AVGYGYRIR 60
           MA+GSKS++    +  LL   +L LF    S F   +ASLP          AVGYGYR+R
Sbjct: 22  MATGSKSLQAFLRRRRLLSAVVLFLF----SCFFRLIASLPFAAGEQRENSAVGYGYRVR 81

Query: 61  STHVDPAGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDH 120
           S  VDP GKSLTADL LI  S VYGPD+  L+LQA+FETKDRLR+RITDS RERWEIPD 
Sbjct: 82  SGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEIPDE 141

Query: 121 IIPRRSNSRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTS 180
           IIPRRSNSRIRSLPE  VGS + S I DPASDLIFSLHDT PFGFSV RRSSGDVLFDTS
Sbjct: 142 IIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTS 201

Query: 181 PDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGS 240
           P+FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFK+VPDK+KTLTLW+ADIGS
Sbjct: 202 PNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWSADIGS 261

Query: 241 RNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGII 300
            N D NLYGAHPFYIDVRS SRDGKVAAGTTHGVLLLNSNGMDIIYSGDR++YK IGGII
Sbjct: 262 VNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGII 321

Query: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYA 360
           DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSDIE+VVARYA
Sbjct: 322 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDIETVVARYA 381

Query: 361 KAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNN 420
           KAGIPLEV+WTDIDYMDGYKDFTFDPINFPV+KMK FVD+LH+NGQKYVLI+DPGISTN 
Sbjct: 382 KAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNK 441

Query: 421 TYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDG 480
           TYGT+IRG + DIFIKYDG+PYLGEVWPG VYFPDFLHPNSE FWGGEI+LFRDIVPFDG
Sbjct: 442 TYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDG 501

Query: 481 LWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHN 540
           LWIDMNE++NFITSSTS FSNLDNPPY I+NAGV+RP+NN+TVPAS LHFGNLTEYNTHN
Sbjct: 502 LWIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHN 561

Query: 541 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILN 600
           LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGY+IPSILN
Sbjct: 562 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILN 621

Query: 601 FGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAAS 660
           FGLFGIPMVGADICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWDTVAAS
Sbjct: 622 FGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAAS 681

Query: 661 AKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSP 720
           A+KVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVLVSP
Sbjct: 682 ARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSP 741

Query: 721 VVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHRE 780
           V+KEGA+SVDAYFPAGNWFSLFNYSE VAVKSGQQITLDAPADHINVH+REGNILALH E
Sbjct: 742 VLKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVHVREGNILALHGE 801

Query: 781 AMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLL 840
           A TT+AAR+TA++LLVV+SNGQSS GEVFLDDGEVVEMG +GGNWSLVRF+SEAVGSKLL
Sbjct: 802 ATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLL 861

Query: 841 VKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAK 900
           +KSQVINGGFALSQK+IIDKVTFVGFERPKKMGGLGL+ISKG NL GNS IRKTY+Y AK
Sbjct: 862 IKSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAK 921

Query: 901 FMNAEISGLSIPIWEEFILEITPIS 916
           F+N EISGLSIPI EEF++E++P++
Sbjct: 922 FVNVEISGLSIPICEEFVMELSPLN 937

BLAST of Clc01G09400 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1570.1 bits (4064), Expect = 0.0e+00
Identity = 772/925 (83.46%), Postives = 834/925 (90.16%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLP----------AVGYGYRIR 60
           MA+GSKS++    +  LL   +L LF     FF L  ASLP          AVGYGYR+R
Sbjct: 1   MATGSKSLQAFLRRRRLLSAVVLFLF---TCFFRL-TASLPFAAGEQRENSAVGYGYRVR 60

Query: 61  STHVDPAGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDH 120
           S  VDP GKSLTADL LI  S VYGPD+  L+LQA+FETKDRLR+RITDS RERWE+PD 
Sbjct: 61  SGRVDPTGKSLTADLDLIDKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDE 120

Query: 121 IIPRRSNSRIRSLPENHVGSTKASFIFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTS 180
           IIPRRSNSRIRSLPE  VGS + S I DPASDLIFSLHDT PFGFSV RRSSGDVLFDTS
Sbjct: 121 IIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTS 180

Query: 181 PDFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGS 240
           P+FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFKLVPDK+KTLTLWNADIGS
Sbjct: 181 PNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGS 240

Query: 241 RNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGII 300
            N D NLYGAHPFYIDVRS SRDGKVAAGTTHGVLLLNSNGMDIIY+GDR++YK IGGII
Sbjct: 241 VNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGII 300

Query: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYA 360
           DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG     FHQCRYGYKNVSDIE+VVARYA
Sbjct: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFG-----FHQCRYGYKNVSDIETVVARYA 360

Query: 361 KAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNN 420
           KAGIPLEV+WTDIDYMDGYKDFTFDPINFPV+KMK FVDNLH+NGQKYVLILDPGISTN 
Sbjct: 361 KAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNK 420

Query: 421 TYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDG 480
           TYGT+IRG + DIFIKYDG+PYLGEVWPG VYFPDFLHPNSE FWG EI+L RDIVPFDG
Sbjct: 421 TYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDG 480

Query: 481 LWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHN 540
           LWIDMNEI+NFITSSTSSFSNLDNPPY INNAGV+RP+NN+TVPAS LHFGNLTEYNTHN
Sbjct: 481 LWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHN 540

Query: 541 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILN 600
           LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGY+IPSILN
Sbjct: 541 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILN 600

Query: 601 FGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAAS 660
           FGLFGIPMVGADICGF GDTTEELC+RWIQ+GAFYPF+RDHSDKGSIRQELYLWDTVAAS
Sbjct: 601 FGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAAS 660

Query: 661 AKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSP 720
           A+KVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVL+SP
Sbjct: 661 ARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISP 720

Query: 721 VVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHRE 780
           V+KEGA+SVDAYFPAGNWFSLFNYSE VA+KSGQQITLDAPADHINVH+REGNILALH E
Sbjct: 721 VLKEGAISVDAYFPAGNWFSLFNYSESVAIKSGQQITLDAPADHINVHVREGNILALHGE 780

Query: 781 AMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLL 840
           A TT+AAR+T ++LLVV+SNGQSS GEVFLDDGE +EMG +GGNWSLVRF+SEAVGSKLL
Sbjct: 781 ATTTQAARKTEFKLLVVVSNGQSSSGEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLL 840

Query: 841 VKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQYFAK 900
           +KSQVINGGFALSQK+IIDKVT VGFERPK MG LGL+ISKG NL GNS IR TY+Y AK
Sbjct: 841 IKSQVINGGFALSQKMIIDKVTIVGFERPKNMGDLGLDISKGANLNGNSGIRITYEYSAK 900

Query: 901 FMNAEISGLSIPIWEEFILEITPIS 916
           F+N +ISGLSIPI E F++E++ ++
Sbjct: 901 FVNVQISGLSIPICEGFVMELSSLN 916

BLAST of Clc01G09400 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 558/899 (62.07%), Postives = 697/899 (77.53%), Query Frame = 0

Query: 18  LFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPAGKSLTADLHLIRTSPVYGPD 77
           +F  +++ F    S  +L       VGYGY +RS  VD   + LTA L LI+ S VY PD
Sbjct: 10  IFIVVVVFFSLRSSQVVLEEEESTVVGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVYAPD 69

Query: 78  LLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRIR--SLPENHVGSTKASF 137
           + SL L  + ET +RLRIRITDS+++RWEIP+ +IPR  N   R  S  E+   S + +F
Sbjct: 70  IKSLNLHVSLETSERLRIRITDSSQQRWEIPETVIPRAGNHSPRRFSTEEDGGNSPENNF 129

Query: 138 IFDPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSETFLVFKDQYIQLSSSLPK 197
           + DP+SDL+F+LH+TTPFGFSV RRSSGD+LFDTSPD SDS T+ +FKDQ++QLSS+LP+
Sbjct: 130 LADPSSDLVFTLHNTTPFGFSVSRRSSGDILFDTSPDSSDSNTYFIFKDQFLQLSSALPE 189

Query: 198 DRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSRNLDTNLYGAHPFYIDVRSRSRDGK 257
           +RS+++GIGE T+ SF+L+P   +T+TLWNADIGS N D NLYG+HPFY+DVR     G 
Sbjct: 190 NRSNLYGIGEHTKRSFRLIP--GETMTLWNADIGSENPDVNLYGSHPFYMDVRGSK--GN 249

Query: 258 VAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 317
             AGTTHGVLLLNSNGMD+ Y G R+TY VIGG+IDLY FAGPSP  V++QYTELIGRPA
Sbjct: 250 EEAGTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPSPEMVMNQYTELIGRPA 309

Query: 318 PVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFD 377
           P+PYWSFG     FHQCRYGYKNVSD+E VV  YAKAGIPLEV+WTDIDYMDGYKDFT D
Sbjct: 310 PMPYWSFG-----FHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLD 369

Query: 378 PINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIRGTKDDIFIKYDGIPYLGE 437
           P+NFP DKM+ FVD LH+NGQKYVLILDPGI  +++YGT+ RG + D+FIK +G PYLGE
Sbjct: 370 PVNFPEDKMQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGE 429

Query: 438 VWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNLDNP 497
           VWPG VYFPDFL+P +  FW  EI++F++I+P DGLWIDMNE++NFITS  SS S+LD+P
Sbjct: 430 VWPGKVYFPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDP 489

Query: 498 PYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVL 557
           PY INN+G +RP+NNKTVPA+ +HFGN++EY+ HNLYG LE+KATH ++V +TGKRPF+L
Sbjct: 490 PYKINNSGDKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFIL 549

Query: 558 SRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELC 617
           SRSTFV SGKYTAHWTGDN A W DL YSIP ILNFGLFGIPMVGADICGF  DTTEELC
Sbjct: 550 SRSTFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELC 609

Query: 618 QRWIQVGAFYPFSRDHSDKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKK 677
           +RWIQ+GAFYPF+RDHS  G+ RQELYLWD+VA+SA+KVL LR RLLP+ YTLMYEAH  
Sbjct: 610 RRWIQLGAFYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVS 669

Query: 678 GTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYS 737
           G PIARPLFFSFPQD KTYEI+SQFL+G  ++VSP +K+GAV+VDAYFPAGNWF LFNYS
Sbjct: 670 GNPIARPLFFSFPQDTKTYEIDSQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYS 729

Query: 738 ELVAVKSGQQITLDAPADHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQSSF 797
             V   SG+ + LD PADH+NVH+REG+I+A+  EA+TT+ AR+T Y+LLVV S  ++  
Sbjct: 730 FAVGGDSGKHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKTPYQLLVVASRLENIS 789

Query: 798 GEVFLDDGEVVEMGADGGN--WSLVRFFSEAVGSKLLVKSQVINGGFALSQKLIIDKVTF 857
           GE+FLDDGE + MGA GGN  W+LV+F     G  ++++S+V+N  +A   K  I KVTF
Sbjct: 790 GELFLDDGENLRMGAGGGNRDWTLVKFRCYVTGKSVVLRSEVVNPEYASKMKWSIGKVTF 849

Query: 858 VGFERPKKMGGLGLNISKGVNLKGNSTIRKTYQY-FAKFMNAEISGLSIPIWEEFILEI 912
           VGFE  + +    +  S+ +     S I+        +F++ E+S LS+ + ++F + +
Sbjct: 850 VGFENVENVKTYEVRTSERLRSPRISLIKTVSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of Clc01G09400 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 813.5 bits (2100), Expect = 1.7e-235
Identity = 424/944 (44.92%), Postives = 601/944 (63.67%), Query Frame = 0

Query: 1   MASGSKSMRTPFSQSLLLFPALLLLFLFPPSFFLLHVASLPAVGYGYRIRSTHVDPAGKS 60
           MAS S S+   FS SLL    L L+  F P+       S   +G GYR+ S    P G  
Sbjct: 1   MASSSSSL--AFSLSLL----LALILCFSPT------QSYKTIGKGYRLVSIEESPDG-G 60

Query: 61  LTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDSTRERWEIPDHIIPRRSNSRI 120
               L + + + +YG D+ +L L    ET  RLR+ ITD+ ++RWE+P +++PR    ++
Sbjct: 61  FIGYLQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQV 120

Query: 121 RSLPENHVGSTKASFIF---DPASDLIFSLHDTTPFGFSVLRRSSGDVLFDTSPDFSDSE 180
             +    +G ++ S I       S+LIFS + T PF F+V RRS+ + LF+T+       
Sbjct: 121 GKV----IGKSRKSPITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTT------- 180

Query: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR-ESFKLVPDKNKTLTLWNADIGSRNLDTN 240
           + LVFKDQY+++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ + NL+T+
Sbjct: 181 SSLVFKDQYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTD 240

Query: 241 LYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFA 300
           LYG+HP Y+D+R+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF A
Sbjct: 241 LYGSHPMYMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIA 300

Query: 301 GPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPL 360
           GPSP++V+DQYT+LIGRPAP+PYWS G     FHQCR+GY N+S +E VV  Y KA IPL
Sbjct: 301 GPSPLNVVDQYTQLIGRPAPMPYWSLG-----FHQCRWGYHNLSVVEDVVDNYKKAKIPL 360

Query: 361 EVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFI 420
           +V+W D D+MDG+KDFT +P+ +P  K+  F+D +H+ G KY++I DPGI  N +YGTF 
Sbjct: 361 DVIWNDDDHMDGHKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQ 420

Query: 421 RGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMN 480
           R    D+FIKY+G P+L +VWPG VYFPDFL+P + ++WG EI+ F D+VP DGLWIDMN
Sbjct: 421 RAMAADVFIKYEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMN 480

Query: 481 EIANF------------------------ITSSTSSFSNLDNPPYMINNAGVRRPLNNKT 540
           E++NF                        +     + +  D+PPY IN  GV  P+  KT
Sbjct: 481 EVSNFCSGLCTIPEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKT 540

Query: 541 VPASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTG 600
           +  S  H+  + EY+ H++YGF E+ ATH  L+ V GKRPF+LSRSTFVGSG+Y AHWTG
Sbjct: 541 IATSATHYNGVREYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTG 600

Query: 601 DNGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHS 660
           DN  TW  L  SI ++LNFG+FG+PMVG+DICGF+   TEELC RWI+VGAFYPFSRDH+
Sbjct: 601 DNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHA 660

Query: 661 DKGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIK 720
           +  S RQELY WDTVA SA+  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  +
Sbjct: 661 NYYSPRQELYQWDTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTE 720

Query: 721 TYEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPA 780
            Y  + QFLLG   ++SPV+++G   V+A FP G+W+ +F+ ++ V  K+G+++TL AP 
Sbjct: 721 CYGNSRQFLLGSSFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPL 780

Query: 781 DHINVHIREGNILALHREAMTTKAARETAYELLVVISNGQS---SFGEVFLDDGEVVEMG 840
           + +NVH+ +  IL   +  + +K AR T + L++    G S   + G+++LD+ E+ EM 
Sbjct: 781 NFVNVHLYQNTILPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMK 840

Query: 841 ADGGNWSLVRFFSEAVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNI 900
              G  + V F++      + + SQV  G FALS+  +I+KV+ +G     ++  + +N 
Sbjct: 841 LGNGQSTYVDFYASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQING 900

Query: 901 SKGVNLKGNSTIRKTY------QYFAKFMNAEISGLSIPIWEEF 908
           S        S+   TY      +   K +  E+ GL + + ++F
Sbjct: 901 SPMTKKIEVSSKEHTYVIGLEDEEENKSVMVEVRGLEMLVGKDF 906

BLAST of Clc01G09400 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 802.0 bits (2070), Expect = 5.1e-232
Identity = 411/883 (46.55%), Postives = 567/883 (64.21%), Query Frame = 0

Query: 42  AVGYGYRIRSTHVDPAGKSLTADLHLIRTSPVYGPDLLSLTLQATFETKDRLRIRITDST 101
           A+G GYR+ S    P   S    L + +++ +YG D+  L L   + T  RLR+ ITD+ 
Sbjct: 23  AIGKGYRLISMEKSPDDGSFIGYLQVKQSNKIYGSDITILRLFINYRTDHRLRVHITDAK 82

Query: 102 RERWEIPDHIIPRRSNSRIRSLPENHVGSTKAS-----FIFDPASDLIFSLHDTTPFGFS 161
           ++RWE+P +++ R         P N +G ++ S      I  P   LIF++    PF F+
Sbjct: 83  KQRWEVPYNLLRREQ-------PPNVIGKSRKSPVTVQEISGPELILIFTV---DPFSFA 142

Query: 162 VLRRSSGDVLFDTSPDFSDSETF--LVFKDQYIQLSSSLPKDRSSIFGIGEQTR-ESFKL 221
           V RRS+G+ +F+TS   S  E+F  +VFKDQY+++S+SLPKD +S++G GE ++    KL
Sbjct: 143 VRRRSNGETIFNTS---SSDESFGEMVFKDQYLEISTSLPKD-ASLYGFGENSQANGIKL 202

Query: 222 VPDKNKTLTLWNADIGSRNLDTNLYGAHPFYIDVRSRSRDGKVAAGTTHGVLLLNSNGMD 281
           VP  N+  TL+  D+ + NL+T+LYG+HP Y+D+R+ S  GK  A   H VLLLNS+GMD
Sbjct: 203 VP--NEPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVS--GKAYA---HSVLLLNSHGMD 262

Query: 282 IIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCR 341
           + Y GD +TYKVIGG+ D YFFAGPSP++V+DQYT LIGRPAP+PYWS G     FHQCR
Sbjct: 263 VFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQYTSLIGRPAPMPYWSLG-----FHQCR 322

Query: 342 YGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFDPINFPVDKMKKFVDNLHE 401
           +GY+NVS ++ VV  Y KA IPL+V+W D DYMDGYKDFT D +NFP  K+  F+D +H+
Sbjct: 323 WGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLDLVNFPHAKLLSFLDRIHK 382

Query: 402 NGQKYVLILDPGISTNNTYGTFIRGTKDDIFIKYDGIPYLGEVWPGLVYFPDFLHPNSEN 461
            G KYV+I DPGI  N +YG + RG   D+FIKY+G P+L +VWPG VYFPDFL+P + +
Sbjct: 383 MGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKPFLAQVWPGPVYFPDFLNPKTVS 442

Query: 462 FWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNLDNPPYMINNAGVRRPLNNKTV 521
           +WG EI+ F ++VP DGLWIDMNE                     IN  G +  L  KT+
Sbjct: 443 WWGDEIRRFHELVPIDGLWIDMNE---------------------INATGHKASLGFKTI 502

Query: 522 PASILHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGD 581
           P S  H+  + EY+ H++YGF E+ ATH +L+ V GKRPF+LSRSTFVGSG+Y AHWTGD
Sbjct: 503 PTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKRPFILSRSTFVGSGQYAAHWTGD 562

Query: 582 NGATWNDLGYSIPSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSD 641
           N  TW  L  SI ++LNFG+FG+PMVG+DICGFF  T EELC RWI+VGAFYPFSRDH+D
Sbjct: 563 NQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRWIEVGAFYPFSRDHAD 622

Query: 642 KGSIRQELYLWDTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKT 701
             + R+ELY W TVA SA+  L +RY+LLP+ YTL YEAH  G PIARPLFFSFP+  + 
Sbjct: 623 YYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMSGAPIARPLFFSFPEFTEC 682

Query: 702 YEINSQFLLGGGVLVSPVVKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPAD 761
           Y ++ QFLLG  +++SPV+++G   V+A FP G+W+ +F+ +++V  K+G+  TL AP +
Sbjct: 683 YGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHMFDMTQVVVSKNGRLFTLPAPFN 742

Query: 762 HINVHIREGNILALHREAMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGG 821
            +NVH+ +  IL + +       A           S G +S G++FLDD E+ EM    G
Sbjct: 743 VVNVHLYQNAILPMQQVVAFPAGA-----------SEGYAS-GKLFLDDDELPEMKLGNG 802

Query: 822 NWSLVRFFSEAVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLGLNISKGV 881
             + + F++      + + SQV  G FALSQ L+I+KV  +G +   K+  + LN S   
Sbjct: 803 KSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLGLKGTWKVSEILLNGSSIS 846

Query: 882 NLKGNSTIRKTYQYF---------AKFMNAEISGLSIPIWEEF 908
           N      +    Q +         +K    E+ GL + + ++F
Sbjct: 863 NETKTIEVSSKEQMYVVGSEDEGESKSFMVELKGLEMLVGKDF 846

BLAST of Clc01G09400 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 328.6 bits (841), Expect = 1.6e-89
Identity = 228/694 (32.85%), Postives = 340/694 (48.99%), Query Frame = 0

Query: 196 DRSSIFGIGEQTRESFKLVPDK------NKTLTLWNADIGSRNLDT--NLYGAHPFYIDV 255
           D S ++GI E    SF L P K      ++   L+N D+   + ++   LYG+ PF +  
Sbjct: 222 DSSFVYGIPEHA-TSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMV-- 281

Query: 256 RSRSRDGKVAAGTTHGVLLLNS---------------NGMDIIYSGDRVT--YKVIGGII 315
            S  + GK     T G   LN+               +G+ +  S  R+   +    GI+
Sbjct: 282 -SHGKSGK-----TSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 316 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYA 375
           D +FF GP P  V+ QY  + G  A    ++ G     +HQCR+ YK+  D+  V +++ 
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATG-----YHQCRWNYKDEEDVAQVDSKFD 401

Query: 376 KAGIPLEVLWTDIDYMDGYKDFTFDPINFP-VDKMKKFVDNLHENGQKYVLILDPGISTN 435
           +  IP +VLW DI++ DG + FT+D + FP  ++M+K    L   G+K V I+DP I  +
Sbjct: 402 EHDIPYDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQK---KLAAKGRKMVTIVDPHIKRD 461

Query: 436 NTYGTFIRGTKDDIFIK-YDGIPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPF 495
           ++Y      T+   ++K   G  + G  WPG   + D L P    +WGG           
Sbjct: 462 DSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRF--------- 521

Query: 496 DGLWIDMNEIANFITSSTSSFS-NLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYN 555
                      N++ S+ S ++ N  N P + N   V       T+P   LH G +    
Sbjct: 522 --------SYKNYVGSTPSLYTWNDMNEPSVFNGPEV-------TMPRDALHVGGVEHRE 581

Query: 556 THNLYGFLESKATHASLV-KVTGK-RPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSI 615
            HN YG+    AT   LV +  GK RPFVLSR+ F G+ +Y A WTGDN A W  L  SI
Sbjct: 582 VHNAYGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSI 641

Query: 616 PSILNFGLFGIPMVGADICGFFGDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLW- 675
           P IL  GL GI   GADI GFFG+   EL  RW QVGA+YPF R H+   + R+E +L+ 
Sbjct: 642 PMILTLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFG 701

Query: 676 DTVAASAKKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGG 735
           +      +  +  RY LLPYFYTL  EA+  G P+ RPL+  FPQD  T+  +  F++G 
Sbjct: 702 ERNTELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGS 761

Query: 736 GVLVSPVVKEGAVSVDAYFPA-GNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGN 795
           G+LV  V  +G      Y P   +W+ L N    V    G+   +DAP + I    + G 
Sbjct: 762 GLLVQGVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGT 821

Query: 796 IL-ALHREAMTTKAARETAYELLVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRF-F 855
           I+    R   ++       Y L+V +++ Q + GE+++DDG+  E     G++   RF F
Sbjct: 822 IIPRKDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRR--GSYIHRRFVF 865

BLAST of Clc01G09400 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 300.4 bits (768), Expect = 4.8e-81
Identity = 202/654 (30.89%), Postives = 316/654 (48.32%), Query Frame = 0

Query: 198 SSIFGIGEQTRESFKLVPDKNKTLTLWNADI-GSRNLDTNLYGAHPFYIDVRSRSRDGKV 257
           +S +G GE + +    +    K +  WN D  G  +  T+LY +HP+ + V        V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 258 AAGTTHGVLL-LNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 317
            A TT    + L   G+  I S    +Y +I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 318 PVPYWSFGKYFYCFHQCRYGYKNVSDIESVVARYAKAGIPLEVLWTDIDYMDGYKDFTFD 377
             P W+ G     +HQCR+ Y +   +  +   +    IP +V+W DIDYMDG++ FTFD
Sbjct: 206 MPPKWALG-----YHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFD 265

Query: 378 PINFPVDKMKKFVDNLHENGQKYVLILDPGISTNNTYGTFIRGTKDDIFI-KYDGIPYLG 437
              FP         +LH NG K + +LDPGI     Y  +  G+K+D++I + DG P+ G
Sbjct: 266 KERFP--DPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTG 325

Query: 438 EVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEIANFITSSTSSFSNLDN 497
           EVWPG   FPD+ +  + ++W   ++ F      DG+W DMNE A F             
Sbjct: 326 EVWPGPCVFPDYTNSKARSWWANLVKEFVS-NGVDGIWNDMNEPAVF------------- 385

Query: 498 PPYMINNAGVRRPLNNKTVPASILHFGN------LTEYNTHNLYGFLESKATHASL-VKV 557
                        +  KT+P + +H G+          + HN+YG L +++T+  + +  
Sbjct: 386 ------------KVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELAD 445

Query: 558 TGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYSIPSILNFGLFGIPMVGADICGFF 617
             KRPFVL+R+ F+GS +Y A WTGDN + W  L  SI  +L  GL G P+ G DI GF 
Sbjct: 446 KNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFA 505

Query: 618 GDTTEELCQRWIQVGAFYPFSRDHSDKGSIRQELYLW-DTVAASAKKVLALRYRLLPYFY 677
           G+ T  L  RW+ VGA +PF R HS+ G+   E + + +      +  L  RY+LLP+FY
Sbjct: 506 GNATPRLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFY 565

Query: 678 TLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVL-VSPVVKEGAVSVDAYFPA 737
           TL Y AH  G P+A P+FF+ P D +   + + FLLG  ++  S +  +G+  +    P 
Sbjct: 566 TLFYIAHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPR 625

Query: 738 GNWFSLFNYSELVAVKSGQQITLDAPADHINVHIREGNILALHREAM-TTKAARETAYEL 797
           G W   F+++             D+  D   ++++ G+I++L    +   + +      L
Sbjct: 626 GIWHR-FDFA-------------DSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTL 678

Query: 798 LVVISNGQSSFGEVFLDDGEVVEMGADGGNWSLVRFFSEAVGSKLLVKSQVING 839
           LV +     + G +F DDG+    G   G + +  + +E   S + VK     G
Sbjct: 686 LVSLDENGKAKGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678