Clc01G00560 (gene) Watermelon (cordophanus) v2

Overview
NameClc01G00560
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionSpermidine synthase
LocationClcChr01: 383420 .. 388668 (+)
RNA-Seq ExpressionClc01G00560
SyntenyClc01G00560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATGCTTCAAATTTTCAATCTCCCATCAATATATATATTCCCCAAATCCCCGTTTCGCTCGTACTTTCATAGCTTCAACAACAATCAAGAAAACCAGAGAAACCCACAGAGAGAGAGAGAGAGAGACAAAGAAAGAGACTGATTGTGTTTGTGTTGCGAGATTCTTCTCTTTGCCTCTCTTTCCCGCTTGTTCGTCTCTTTCCCAGAACTTGAAAAGTTCCCATTTCCAGAAATTAACGCCTGGTAATCTTCAACTCTGCTTTGATTGTTCTTGCTCTGTAATCCCATTTCTCTCCGTCTCTCTCGATGCTTTTTGTTTACTTTGATTTTGTTGCTTATATGCATTTTCTCCTCTTTGAACCATCTGGGTTCTTTGTGTTTGTGTTTTCGGCTATTCTGTTTGTTTCTTCGGATATTATATTATTCTCTGTTTTTGTTCTGGCATTTTTGTATGTATATCTCGTACGGTACTTCTTCTTGGTCTTCTCGTCTACCCCAGCAACTGGTCTTAGTCTTACAGGGCTTCGACTGAAATTCCCTTCCACCGTTTTGGAATTACGATTTTTTTCATTCCTTTCTTTTTTAATCGTTGAGCCATGCTGTTTCAGAGATTGTTGGTTAACATCGTTCTATTTCTATATAAGCGTTGGAAAATTATATTTATCGCATTAAATGTAATTATTTTCAAAGTTTGGGGCCCAATGAATTGCTAAATGGTGAGGGAAGGGCGTCGTATTCCTTACAGTGTTTTAATATTCTGGTTTCAAATTACTTGCCATATTTGGTTATTTCCGACGTTAAATCAAAAGGAAGCTAATAAGTATTTATGGAGAATAAAATTTTCTTTGATATGATGTATTAAAACGGAATCAATGTTGTAATCTGGAAGTTACTGTCTTTTCCTTTGAGAGAAGATTATTCTAAGTTTCCTTTTCCAACATTAGGGTGATTTGTATTAAATGTTAAAAGCAGACGTTGGCTTTGGTTTTGGTTTTGGTTTATGATCAGCTGGTATATTTCCTCATCCTCTGAGACTACTTTTTGAACGAGATTTTTCTTCATTCTCACTTTGTTGTTATTTCTCACTTTGGTTGTTCTAATATAATGATTTCTTAGAATTCTTAATTATAAGATGAAGATGATTGCTGGAACTAACTAGTCTGGTCTCTTTCTCATGGATAAACTTGAATGTATAGAGACTGGAGCTCGAGAAGAAAAGGATCATTTATGGAAGCCGACGCAGGAAGAGGTTTGGGATGCCAGAAGACTATGGATGGGAAGGAGAGTAATGGGATTCTTCCAAAGAAGGAGATCCCTTCTTGTTGCTTAAAGGCTAGGGCTTCAGTCCCTGAGCTTGAGGTCAAGTGCCATTCCACTGTTGTTTCTGGGTGGTTCTCAGAACCTCAGCTTGCTTCTGGTCGTTATCTATGCTTGTTTCTTGCTTAATTAAATTTTGGTTTTTTCGTTCATTTTGCTCTAATGAAATCCTTCGTTGGCTTATTTTTATGTCAATATCTTCCAAATTCAGATAACGGAAAGAAAAGGGTCTATTTCAACAATCCCATGTGGCCTGGTAACCTTCTCTACTTTTTTGCTTTGTTTTTTGACAGCTTATGTTATTGCCTATCTGATGAACATCGTTATCACAGGAGGTTGAATAGTTCAAGCACTGTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGTGTTTGAATGCTTTTAGCAATATTATGAAGACTAGTCAGTGTAGTGTGTAAGAGTCTTGAGGAAACCAATATAACATTGATATTTCTCCCATTCTCTTGGAGGGAATATAAACTGTTGATACTTCATGATGGATCATACTTAAATTAGGGTTAATATTATTTTCACAACTTTCATACTTATTATGTTTTGGTTCTTAAATTTTTTGATGTCGTACTTTGGCCCATGAGCTTTGCATGAAAACTATTTTGGTCATTATTGTTGATTTTCCGTAAATATATGTGAGGAAAACCTGCGTGACAAATCTCTTTTTAATGTGTATGATAAGATGTAGCTGGTAAGGAAGTACTTGAGCATAGCAAGCCAAGTTTCAGGCTACAAAATGAGTCAGTTTCTATAAGATAATTAATGAATTATTGACAACAAAGATTTAAATAATTTTTTATGCAGATCTTGAAGTGATATAGAATATTTGAATATTTAGGGACTTAATGAAATAAGTTTGGAAGCTTAGGAATCAAAATAGGATCGATTAATTGTTGATATATGTTATGATAATGTTTGCTACAATATTCTGACTGTTAATATGAGTTATGCATTCTTACCTCAAGCACAATTATGGCCCCTCTATTTCTGTTTGTTTTTAGGAGAAGCACACTCCCTCCAAGTAGAGTCTATTTTATTTAAAGGGAAGTCAGAGTACCAAGAAGTGGTTGTTTTTGAGGTATATTTTATTTCGCATACTGTCCCCTATCGCATCATCCTGTGGTTTCCCAAAGGAAGTATACAGCGAGGTAACTAATGCCGTGATTTTTCATTGACAGTCCACTACATATGGGAAAGTACTTGTTCTAGATGGTATTGTACAGTTAACTGAGAAAGATGAGTGTGCCTATCAGGAGATGATTACTCATCTGCCCCTCTGTTCAATTCCATCTCCCAAAACGGTGACTTCAAATATCCAAACCATGGTGCTTTTGTCTTTATAGTTGTAGTACTTCCATTTTGTCATGCAACAAGAATGGGGGATGATGGAATGGATTATTTGTTTTCGAGAGTTTTGATTTGATAAGTAAATCACTCTGCGCATTGACAATTTTTCTAATCTATCCTATTTATTATGAAAAAGGTGATTACAGTTGATATGGGATTAGCCATTCAATTTGAACTGTTTTGATTCTTTTCTTTCTCCTGATTGGCTGTTATCAGGGGTTTGCATCAAGTAGTTCACTCGATAATTAAGAGTCCGTTTGGATTGATTAGAGAAAAAAGTGTTTTGCAAAAAACTCATTTTTATTTAAACTCTTTTGATAAAAATTGTTTAAAATACACTTCAAAATCTATTTTGAATGGTTGCCAAACACTCCAATTTTTTCCAAAATGATTTATTTTTTAAATTAAACACTTGAAAATGTATTCCAAACACACCCCAAGTCTTTTTATTTCTGTTTCAAACTCATTTTACACTATGACTACTTATTTATGCTTAGCAGTATGGATTTCTGTCATCACTTGAAGATTAAGTTATGTATGTCCAATCCAATAAAACAGTACGTAATAACTAATATTTAGAAAACTAAATGGGTGAAAGAGATTCTTCAACATTTTGGTATATAGCATCCCTATTTTTTAAGCATGTCATCAATGGCAATCTGTTCCTTTTGGTCTAAGCATTGTCTAATGAATATTCACTCTTGTCAGATAGAATGTAAAGGGAAAATATAAACTAATGAGAAAATGTTGCAGGTTCTTGTTGTTGGTGGTGGTGATGGTGGGGTTCTGAGTGAAATCTCTCGCCACAACTCTGTAGAGCATATAGACATATGTGAGATAGATAAGATGGTCATTGATGTATGATATGTAACTATCGCCCTTGTGACAAACAATCACTGGAGTTTTTCTCAAAAGTTAACCACATTGTTGCACTGGTTTATTCACCTATAGGTATCTAAGGAGTTTTTTCCTGATTTAGCTATAGGATTCGAGGATCCCCGCGTCCACCTTCACATTGGTGATGGTATGCCTTAATATTTTTTCTCTTTTGTTTTATTTATTTATTTTTCTATTTTTCCAATATCAATTAATTGGCTTGATATTGAAAATCATTGTGCTATATGCCCGTTCTTATCTCTTAGTATTGTGATTTTTACAGCTGTTGAATTCCTAAGGCGTGCACCAAGAGGGAAATATGATGCAATCATCGTTGATTCCTCAGATCCTGTTGGTATGTTTGTACTTCATTTGATATGGAATTTGTACAACTCATTTATAACAAGACATTTCAGTATATTATTTAATATGAAAACTAGAGAAAAAGTGCAGAGATTTAGTACGGAGTGGTCTTAAAACACAATATCATCCATGAAATGGATGGATGTGGGCTTTTAGTAGGGAGTAACCATACCTTTATCTAATTTTCAATTACTTGTGGAACTGCTCCATAAATGTTCTCTAGTTTATGCCTACTCATCCATCACAAAGTTGCTTGCTATTTTAGGGCCTGCTCAGGAGCTAGTTGAGAAGCCATTCTTTGAGACAATAGCAAAAGCATTGAAGCCTGGTGGTGTCCTCTGTAACATGGCAGAAAGTATGTGGCTACATACACATCTTATTGAAGATATGATCTCTATATGCCGTGAAATTTTCAAAGGATCTGTTTATTATGCATGGGGGAGCGTTCCAACTTATCCAAGGTCATTAACTAGCTGCTTTTTATTTTATTTTATTTTTATTTTTATCGTTATAAATAATTTGCCTAGGGGGATATTCTTATTTTTCAATCTGTAAAGTTGAGATGCACGTGAAGGCTAAGAGTTGCTGTCCGACTTAAAAAGAATTCATTTTGTTTTTTTCCATTTTCAGACGATTATTGTTCTTTCTCAACTTTGGGTTTTGTTTTCTTCAGTGGTGTGATAGGTTTTTTGCTATGTTCAACCGAAGGGCCACCTGTTGATTTTAAAAATCCTATTAATCCCATTGAGAAGTTAGAAGGTGCAGTCAAGCATAAAAGAGATCTCAAGTTCTACAATTCAGAGGTACATTTATGTTTTTCGTTCTTTTCAAAAAAAAAAAAAAAAAGAAAAGAAAAAAAAAAAAAACCAGCGACTGCTATGCTGTCAGTTGTAGCATAGAACATGTGGATAAATTCAAGCTCTAGAAATCAAATTCACATCTTAAATGTACTCTATAAAAGTAGACTAAGAATATAAGGGCATAAAAAAATTCCTAAAAAGTGGTTTCTTACACCCCCTCAAAAAGAAAAAAGGAGGCTAATGTTTTAGTTGGAAAAGTTTTTTCAAAGAGGATGCAAGTTCAACTGATATTAACTGTTGCTGAATATTGGCACCAGACTTCATCCCTCGACCCTGCAAGCTGTAGAAGTCATTTTAACTTCCTACAGGCTAACAGCCTTTCACATCCAATGTGTTTGTGAATAACAAAAACTTGTTTGTTTGACAGATACACTCGGCTGCTTTTGCCTTGCCATCATTTCTGAGGAAGGAGGTGAAGGCACTTGCTGATTCTCCAAACCCAGCACAAAACTGA

mRNA sequence

CGATGCTTCAAATTTTCAATCTCCCATCAATATATATATTCCCCAAATCCCCGTTTCGCTCGTACTTTCATAGCTTCAACAACAATCAAGAAAACCAGAGAAACCCACAGAGAGAGAGAGAGAGAGACAAAGAAAGAGACTGATTGTGTTTGTGTTGCGAGATTCTTCTCTTTGCCTCTCTTTCCCGCTTGTTCGTCTCTTTCCCAGAACTTGAAAAGTTCCCATTTCCAGAAATTAACGCCTGAGACTGGAGCTCGAGAAGAAAAGGATCATTTATGGAAGCCGACGCAGGAAGAGGTTTGGGATGCCAGAAGACTATGGATGGGAAGGAGAGTAATGGGATTCTTCCAAAGAAGGAGATCCCTTCTTGTTGCTTAAAGGCTAGGGCTTCAGTCCCTGAGCTTGAGGTCAAGTGCCATTCCACTGTTGTTTCTGGGTGGTTCTCAGAACCTCAGCTTGCTTCTGATAACGGAAAGAAAAGGGTCTATTTCAACAATCCCATGTGGCCTGGAGAAGCACACTCCCTCCAAGTAGAGTCTATTTTATTTAAAGGGAAGTCAGAGTACCAAGAAGTGGTTGTTTTTGAGTCCACTACATATGGGAAAGTACTTGTTCTAGATGGTATTGTACAGTTAACTGAGAAAGATGAGTGTGCCTATCAGGAGATGATTACTCATCTGCCCCTCTGTTCAATTCCATCTCCCAAAACGGTTCTTGTTGTTGGTGGTGGTGATGGTGGGGTTCTGAGTGAAATCTCTCGCCACAACTCTGTAGAGCATATAGACATATGTGAGATAGATAAGATGGTCATTGATGTATCTAAGGAGTTTTTTCCTGATTTAGCTATAGGATTCGAGGATCCCCGCGTCCACCTTCACATTGGTGATGCTGTTGAATTCCTAAGGCGTGCACCAAGAGGGAAATATGATGCAATCATCGTTGATTCCTCAGATCCTGTTGTTTATGCCTACTCATCCATCACAAAGTTGCTTGCTATTTTAGGGCCTGCTCAGGAGCTAGTTGAGAAGCCATTCTTTGAGACAATAGCAAAAGCATTGAAGCCTGGTGGTGTCCTCTGTAACATGGCAGAAAGTATGTGGCTACATACACATCTTATTGAAGATATGATCTCTATATGCCGTGAAATTTTCAAAGGATCTGTTTATTATGCATGGGGGAGCGTTCCAACTTATCCAAGTGGTGTGATAGGTTTTTTGCTATGTTCAACCGAAGGGCCACCTGTTGATTTTAAAAATCCTATTAATCCCATTGAGAAGTTAGAAGGTGCAGTCAAGCATAAAAGAGATCTCAAGTTCTACAATTCAGAGATACACTCGGCTGCTTTTGCCTTGCCATCATTTCTGAGGAAGGAGGTGAAGGCACTTGCTGATTCTCCAAACCCAGCACAAAACTGA

Coding sequence (CDS)

ATGGAAGCCGACGCAGGAAGAGGTTTGGGATGCCAGAAGACTATGGATGGGAAGGAGAGTAATGGGATTCTTCCAAAGAAGGAGATCCCTTCTTGTTGCTTAAAGGCTAGGGCTTCAGTCCCTGAGCTTGAGGTCAAGTGCCATTCCACTGTTGTTTCTGGGTGGTTCTCAGAACCTCAGCTTGCTTCTGATAACGGAAAGAAAAGGGTCTATTTCAACAATCCCATGTGGCCTGGAGAAGCACACTCCCTCCAAGTAGAGTCTATTTTATTTAAAGGGAAGTCAGAGTACCAAGAAGTGGTTGTTTTTGAGTCCACTACATATGGGAAAGTACTTGTTCTAGATGGTATTGTACAGTTAACTGAGAAAGATGAGTGTGCCTATCAGGAGATGATTACTCATCTGCCCCTCTGTTCAATTCCATCTCCCAAAACGGTTCTTGTTGTTGGTGGTGGTGATGGTGGGGTTCTGAGTGAAATCTCTCGCCACAACTCTGTAGAGCATATAGACATATGTGAGATAGATAAGATGGTCATTGATGTATCTAAGGAGTTTTTTCCTGATTTAGCTATAGGATTCGAGGATCCCCGCGTCCACCTTCACATTGGTGATGCTGTTGAATTCCTAAGGCGTGCACCAAGAGGGAAATATGATGCAATCATCGTTGATTCCTCAGATCCTGTTGTTTATGCCTACTCATCCATCACAAAGTTGCTTGCTATTTTAGGGCCTGCTCAGGAGCTAGTTGAGAAGCCATTCTTTGAGACAATAGCAAAAGCATTGAAGCCTGGTGGTGTCCTCTGTAACATGGCAGAAAGTATGTGGCTACATACACATCTTATTGAAGATATGATCTCTATATGCCGTGAAATTTTCAAAGGATCTGTTTATTATGCATGGGGGAGCGTTCCAACTTATCCAAGTGGTGTGATAGGTTTTTTGCTATGTTCAACCGAAGGGCCACCTGTTGATTTTAAAAATCCTATTAATCCCATTGAGAAGTTAGAAGGTGCAGTCAAGCATAAAAGAGATCTCAAGTTCTACAATTCAGAGATACACTCGGCTGCTTTTGCCTTGCCATCATTTCTGAGGAAGGAGGTGAAGGCACTTGCTGATTCTCCAAACCCAGCACAAAACTGA

Protein sequence

MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQLASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALPSFLRKEVKALADSPNPAQN
Homology
BLAST of Clc01G00560 vs. NCBI nr
Match: XP_004145928.1 (spermine synthase [Cucumis sativus] >KGN49872.1 hypothetical protein Csa_000091 [Cucumis sativus])

HSP 1 Score: 714.9 bits (1844), Expect = 3.6e-202
Identity = 344/379 (90.77%), Postives = 358/379 (94.46%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDG+ SNGIL KKEIPSCCLKARASVPELE KCHSTVVSGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVVSGWFSEPQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            A D+GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVLDGIVQL
Sbjct: 61  FAYDDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNS+EHIDICEID+MVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSIEHIDICEIDQMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSKEFFPDLAIGFEDPRVHLH+GDAVEFLRRAPRGKYDAIIVDSSDPV            
Sbjct: 181 VSKEFFPDLAIGFEDPRVHLHVGDAVEFLRRAPRGKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFET+AKALKPGGVLCNMAESMWLHTHLI+DMISICRE+FKGS++YAW
Sbjct: 241 --GPAQELVEKPFFETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKGSIHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
            SVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHK+DLKFYNSE+HSAAFALP
Sbjct: 301 ASVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKKDLKFYNSEMHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLRKEVKAL DSPNP+QN
Sbjct: 361 SFLRKEVKALVDSPNPSQN 365

BLAST of Clc01G00560 vs. NCBI nr
Match: XP_038906466.1 (spermine synthase [Benincasa hispida])

HSP 1 Score: 711.1 bits (1834), Expect = 5.2e-201
Identity = 348/379 (91.82%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDGK SNGIL KKEIPSCCLKARAS PELE KCHSTVVSGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGKGSNGILTKKEIPSCCLKARASGPELEAKCHSTVVSGWFSEPQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            A D+GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVLDGIVQL
Sbjct: 61  FAYDDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSKEFFPDLAIGFEDPRVHLH+GDAVEFLRRAPRGKYDAIIVDSSDPV            
Sbjct: 181 VSKEFFPDLAIGFEDPRVHLHVGDAVEFLRRAPRGKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLI+DMISICREIFKGSV+YAW
Sbjct: 241 --GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIDDMISICREIFKGSVHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
            SVPTYPSGVIGFLLCSTEGP VDFKNPINPIEKLEGAVKHKRDLKFYNSE+HSAAFALP
Sbjct: 301 ASVPTYPSGVIGFLLCSTEGPLVDFKNPINPIEKLEGAVKHKRDLKFYNSEMHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLRKEVKAL +SPNP QN
Sbjct: 361 SFLRKEVKALVNSPNPEQN 365

BLAST of Clc01G00560 vs. NCBI nr
Match: XP_008437587.1 (PREDICTED: spermine synthase [Cucumis melo] >XP_008437588.1 PREDICTED: spermine synthase [Cucumis melo])

HSP 1 Score: 703.7 bits (1815), Expect = 8.4e-199
Identity = 339/379 (89.45%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDG+ SNGIL KKEIPSCCLKARASVPELE KCHSTV+SGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVISGWFSEPQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            A D+GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVLDGIVQL
Sbjct: 61  FAYDDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEID+MVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDQMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSK+FFPDLA GFEDPRVHLH+GDAVEFLRRAP GKYDAIIVDSSDPV            
Sbjct: 181 VSKKFFPDLARGFEDPRVHLHVGDAVEFLRRAPSGKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFET+AKALKPGGVLCNMAESMWLHTHLI+DMISICRE+FKGS++YAW
Sbjct: 241 --GPAQELVEKPFFETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKGSIHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
            SVPTYPSGVIGFLLCSTEG PVDFKNPINPIEKLEG VKHK+DLKFYNSE+HSAAFALP
Sbjct: 301 ASVPTYPSGVIGFLLCSTEGKPVDFKNPINPIEKLEGGVKHKKDLKFYNSEMHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLRKEVKAL DSPNP+QN
Sbjct: 361 SFLRKEVKALVDSPNPSQN 365

BLAST of Clc01G00560 vs. NCBI nr
Match: KAA0042556.1 (spermine synthase [Cucumis melo var. makuwa] >TYK05960.1 spermine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 694.5 bits (1791), Expect = 5.1e-196
Identity = 338/385 (87.79%), Postives = 353/385 (91.69%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDG+ SNGIL KKEIPSCCLKARASVPELE KCHSTV+SGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVISGWFSEPQ 60

Query: 61  LASD------NGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVL 120
            A        +GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVL
Sbjct: 61  FAYGRYLSLYDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVL 120

Query: 121 DGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEI 180
           DGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEI
Sbjct: 121 DGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEI 180

Query: 181 DKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSS 240
           D+MVIDVSK+FFPDLA GFEDPRVHLH+GDAVEFLRRAP GKYDAIIVDSSDPV      
Sbjct: 181 DQMVIDVSKKFFPDLARGFEDPRVHLHVGDAVEFLRRAPSGKYDAIIVDSSDPV------ 240

Query: 241 ITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKG 300
                   GPAQELVEKPFFET+AKALKPGGVLCNMAESMWLHTHLI+DMISICRE+FKG
Sbjct: 241 --------GPAQELVEKPFFETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKG 300

Query: 301 SVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHS 360
           S++YAW SVPTYPSGVIGFLLCSTEG PVDFKNPINPIEKLEG VKHK+DLKFYNSE+HS
Sbjct: 301 SIHYAWASVPTYPSGVIGFLLCSTEGKPVDFKNPINPIEKLEGGVKHKKDLKFYNSEMHS 360

Query: 361 AAFALPSFLRKEVKALADSPNPAQN 380
           AAFALPSFLRKEVKAL DSPNP+QN
Sbjct: 361 AAFALPSFLRKEVKALVDSPNPSQN 371

BLAST of Clc01G00560 vs. NCBI nr
Match: KAG7035744.1 (Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681.4 bits (1757), Expect = 4.4e-192
Identity = 328/379 (86.54%), Postives = 350/379 (92.35%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDGK SNGI+PKKEIPSCCLKA AS PEL+ KCHSTVVSGWFSE Q
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            ASD G K VYFNNPMWPGEAHSLQVESILF+G+SEYQEVVVFEST+YGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVL E++RHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSKEFFP+LA GFEDPRVHLH+GDAV+FLRRAP+ KYDAIIVDSSDPV            
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLI+DMISICR+IFKGSV+YAW
Sbjct: 241 --GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
           GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLE + +++R+LKFYNSEIHSAAFALP
Sbjct: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLR+EVKAL DSP+PAQ+
Sbjct: 361 SFLRREVKALVDSPDPAQD 365

BLAST of Clc01G00560 vs. ExPASy Swiss-Prot
Match: Q94BN2 (Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 5.7e-166
Identity = 288/374 (77.01%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           ME D G GL CQ TMDGK SNG   +K +PSCCLKA A VPE + KCHSTVVSGWFSEP 
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  LAS-DNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQ 120
             S   G K VYFNNPMWPGEAHSL+VE +LFK KS++QEV+VFES TYGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVL EISRH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLL 240
           DVSK+FFP+LA+GF+DPRV LHIGDA EFLR++P GKYDAIIVDSSDPV           
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPV----------- 240

Query: 241 AILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYA 300
              GPA  LVEKPFFET+A+ALKPGGVLCNMAESMWLHTHLIEDMISICR+ FK SV+YA
Sbjct: 241 ---GPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 300

Query: 301 WGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFAL 360
           W SVPTYPSGVIGF+LCSTEGP VDFKNPINPIEKL+GA+ HKR+LKFYNS++H AAFAL
Sbjct: 301 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 359

Query: 361 PSFLRKEVKALADS 374
           P+FLR+EV +L  S
Sbjct: 361 PTFLRREVASLLAS 359

BLAST of Clc01G00560 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.5e-129
Identity = 220/324 (67.90%), Postives = 261/324 (80.56%), Query Frame = 0

Query: 49  STVVSGWFSEPQLASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTY 108
           S+V+ GWFSE               +PMWPGEAHSL+VE ILF+GKS+YQ V+VF+S+TY
Sbjct: 21  SSVIPGWFSE--------------ISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTY 80

Query: 109 GKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEH 168
           GKVLVLDG++QLTE+DECAYQEMI HLPLCSIPSPK VLV+GGGDGGVL E++RH SVE 
Sbjct: 81  GKVLVLDGVIQLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARHLSVEQ 140

Query: 169 IDICEIDKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPV 228
           IDICEIDKMV+DVSK+FFPD+A+GFEDPRV LHIGD V FL+  P G YDAIIVDSSDP+
Sbjct: 141 IDICEIDKMVVDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPI 200

Query: 229 VYAYSSITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISIC 288
                         GPAQEL EKPFFE++AKAL+PGGV+C  AES+WLH H+IED+++ C
Sbjct: 201 --------------GPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANC 260

Query: 289 REIFKGSVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFY 348
           R+IFKGSV YAW +VPTYPSGVIGF+LCSTEGPPVDFK+PINPI+  +G  K  + LKFY
Sbjct: 261 RQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPINPIDANDGRSKTMKPLKFY 316

Query: 349 NSEIHSAAFALPSFLRKEVKALAD 373
           NSEIHSAAF LPSF +K + + A+
Sbjct: 321 NSEIHSAAFCLPSFAKKVIDSKAN 316

BLAST of Clc01G00560 vs. ExPASy Swiss-Prot
Match: O48659 (Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.0e-127
Identity = 215/323 (66.56%), Postives = 260/323 (80.50%), Query Frame = 0

Query: 49  STVVSGWFSEPQLASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTY 108
           S+++ GWFSE               +P+WPGEAHSL+VE ILF+GKS+YQ VVVF+S+TY
Sbjct: 13  SSILPGWFSE--------------ISPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTY 72

Query: 109 GKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEH 168
           GKVLVLDG++QLTE+DECAYQEMITHLPLCSIP+PK VLV+GGGDGGVL E+SRH+SVE 
Sbjct: 73  GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQ 132

Query: 169 IDICEIDKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPV 228
           IDICEIDKMVIDVSK+FFP++AIG+EDPRV LH+GD V FL+  P G YDA+IVDSSDP+
Sbjct: 133 IDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPI 192

Query: 229 VYAYSSITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISIC 288
                         GPAQEL EKPFFE++A+AL PGGV+C  AES+WLH H+IED++S C
Sbjct: 193 --------------GPAQELFEKPFFESVARALCPGGVVCTQAESIWLHMHIIEDIVSNC 252

Query: 289 REIFKGSVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFY 348
           R+IFKGSV YAW +VPTYPSGVIGF+LCSTEGP VDFKNPINPI+  +   K +  LKFY
Sbjct: 253 RQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKNPINPIDADDSHTKTRGPLKFY 307

Query: 349 NSEIHSAAFALPSFLRKEVKALA 372
           NSEIHSA+F LPSF ++ +++ A
Sbjct: 313 NSEIHSASFCLPSFAKRVIESNA 307

BLAST of Clc01G00560 vs. ExPASy Swiss-Prot
Match: Q9ZUB3 (Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.6e-126
Identity = 214/329 (65.05%), Postives = 262/329 (79.64%), Query Frame = 0

Query: 44  EVKCHSTVVSGWFSEPQLASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVF 103
           E  C STV+ GWFSE               +PMWPGEAHSL+VE +LF+GKS+YQ+V+VF
Sbjct: 36  EPACFSTVIPGWFSE--------------MSPMWPGEAHSLKVEKVLFQGKSDYQDVIVF 95

Query: 104 ESTTYGKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRH 163
           +S TYGKVLVLDG++QLTE+DECAYQEMITHLPLCSIP+PK VLV+GGGDGGVL E++RH
Sbjct: 96  QSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARH 155

Query: 164 NSVEHIDICEIDKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVD 223
            S+E ID+CEIDKMV+DVSK+FFPD+AIG+EDPRV+L IGD V FL+ A  G YDA+IVD
Sbjct: 156 ASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVD 215

Query: 224 SSDPVVYAYSSITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIED 283
           SSDP+              GPA+EL EKPFF+++A+AL+PGGV+C  AES+WLH  +IED
Sbjct: 216 SSDPI--------------GPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIED 275

Query: 284 MISICREIFKGSVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKR 343
           ++S CREIFKGSV YAW SVPTYPSGVIGF+LCSTEGP VDFK+P+NPI+  E + K   
Sbjct: 276 IVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNPID--ESSSKSNG 334

Query: 344 DLKFYNSEIHSAAFALPSFLRKEVKALAD 373
            LKFYN+EIHSAAF LPSF +K +++ A+
Sbjct: 336 PLKFYNAEIHSAAFCLPSFAKKVIESKAN 334

BLAST of Clc01G00560 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.7e-125
Identity = 209/321 (65.11%), Postives = 259/321 (80.69%), Query Frame = 0

Query: 49  STVVSGWFSEPQLASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTY 108
           S+++ GWFSE               +P+WPGEAHSL+VE ILF+GKS+YQ V+VF+S+TY
Sbjct: 13  SSILPGWFSE--------------ISPLWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTY 72

Query: 109 GKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEH 168
           GKVL+LDG++QLTE+DECAYQEMITHLPLCSIP+PK VLV+GGGDGGVL E+SRH+SVE 
Sbjct: 73  GKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQ 132

Query: 169 IDICEIDKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPV 228
           IDICEIDKMVIDVSK+FFP++AIG+EDPRV LH+GD V FL+    G YDA+IVDSSDP+
Sbjct: 133 IDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKFVAEGTYDAVIVDSSDPI 192

Query: 229 VYAYSSITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISIC 288
                         GPAQEL EKPFFE++A+AL+PGGV+C  AES+WLH H+IED+++ C
Sbjct: 193 --------------GPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDIVANC 252

Query: 289 REIFKGSVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFY 348
           R+IFKGSV YAW +VPTYPSGVIGF+LCSTEGP VDFKNPINP++  +   K +  LKFY
Sbjct: 253 RQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKNPINPVDADDSHTKTRGPLKFY 305

Query: 349 NSEIHSAAFALPSFLRKEVKA 370
           NSEIHSA+F LPSF ++ +++
Sbjct: 313 NSEIHSASFCLPSFAKRVIES 305

BLAST of Clc01G00560 vs. ExPASy TrEMBL
Match: A0A0A0KN88 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139500 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.8e-202
Identity = 344/379 (90.77%), Postives = 358/379 (94.46%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDG+ SNGIL KKEIPSCCLKARASVPELE KCHSTVVSGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVVSGWFSEPQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            A D+GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVLDGIVQL
Sbjct: 61  FAYDDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNS+EHIDICEID+MVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSIEHIDICEIDQMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSKEFFPDLAIGFEDPRVHLH+GDAVEFLRRAPRGKYDAIIVDSSDPV            
Sbjct: 181 VSKEFFPDLAIGFEDPRVHLHVGDAVEFLRRAPRGKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFET+AKALKPGGVLCNMAESMWLHTHLI+DMISICRE+FKGS++YAW
Sbjct: 241 --GPAQELVEKPFFETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKGSIHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
            SVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHK+DLKFYNSE+HSAAFALP
Sbjct: 301 ASVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKKDLKFYNSEMHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLRKEVKAL DSPNP+QN
Sbjct: 361 SFLRKEVKALVDSPNPSQN 365

BLAST of Clc01G00560 vs. ExPASy TrEMBL
Match: A0A1S3AUE0 (spermine synthase OS=Cucumis melo OX=3656 GN=LOC103482957 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.0e-199
Identity = 339/379 (89.45%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDG+ SNGIL KKEIPSCCLKARASVPELE KCHSTV+SGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVISGWFSEPQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            A D+GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVLDGIVQL
Sbjct: 61  FAYDDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEID+MVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDQMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSK+FFPDLA GFEDPRVHLH+GDAVEFLRRAP GKYDAIIVDSSDPV            
Sbjct: 181 VSKKFFPDLARGFEDPRVHLHVGDAVEFLRRAPSGKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFET+AKALKPGGVLCNMAESMWLHTHLI+DMISICRE+FKGS++YAW
Sbjct: 241 --GPAQELVEKPFFETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKGSIHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
            SVPTYPSGVIGFLLCSTEG PVDFKNPINPIEKLEG VKHK+DLKFYNSE+HSAAFALP
Sbjct: 301 ASVPTYPSGVIGFLLCSTEGKPVDFKNPINPIEKLEGGVKHKKDLKFYNSEMHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLRKEVKAL DSPNP+QN
Sbjct: 361 SFLRKEVKALVDSPNPSQN 365

BLAST of Clc01G00560 vs. ExPASy TrEMBL
Match: A0A5A7TKT1 (Spermine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00540 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.5e-196
Identity = 338/385 (87.79%), Postives = 353/385 (91.69%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDG+ SNGIL KKEIPSCCLKARASVPELE KCHSTV+SGWFSEPQ
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVISGWFSEPQ 60

Query: 61  LASD------NGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVL 120
            A        +GKKRVYFNNPMWPGEAHSLQVESILFKGKSE+QEVVVFESTTYGKVLVL
Sbjct: 61  FAYGRYLSLYDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVL 120

Query: 121 DGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEI 180
           DGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEI
Sbjct: 121 DGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEI 180

Query: 181 DKMVIDVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSS 240
           D+MVIDVSK+FFPDLA GFEDPRVHLH+GDAVEFLRRAP GKYDAIIVDSSDPV      
Sbjct: 181 DQMVIDVSKKFFPDLARGFEDPRVHLHVGDAVEFLRRAPSGKYDAIIVDSSDPV------ 240

Query: 241 ITKLLAILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKG 300
                   GPAQELVEKPFFET+AKALKPGGVLCNMAESMWLHTHLI+DMISICRE+FKG
Sbjct: 241 --------GPAQELVEKPFFETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKG 300

Query: 301 SVYYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHS 360
           S++YAW SVPTYPSGVIGFLLCSTEG PVDFKNPINPIEKLEG VKHK+DLKFYNSE+HS
Sbjct: 301 SIHYAWASVPTYPSGVIGFLLCSTEGKPVDFKNPINPIEKLEGGVKHKKDLKFYNSEMHS 360

Query: 361 AAFALPSFLRKEVKALADSPNPAQN 380
           AAFALPSFLRKEVKAL DSPNP+QN
Sbjct: 361 AAFALPSFLRKEVKALVDSPNPSQN 371

BLAST of Clc01G00560 vs. ExPASy TrEMBL
Match: A0A6J1K516 (spermine synthase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491249 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 6.3e-192
Identity = 326/379 (86.02%), Postives = 350/379 (92.35%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDGK SNGI+PKKEIPSCCLKA AS PEL+ KCHSTVVSGWFSE Q
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            ASD G K VYFNNPMWPGEAHSLQVESILF+ +SEYQEVVVFEST+YGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQRRSEYQEVVVFESTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVL E++RHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSKEFFP+LA GFEDPRVHLH+GDA++FLRRAP+ KYDA+IVDSSDPV            
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAIDFLRRAPKEKYDAVIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLI+DMISICR+IFKGSV+YAW
Sbjct: 241 --GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
           GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEG+ +++R+LKFYNSEIHSAAFALP
Sbjct: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGSAEYERELKFYNSEIHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLR+EVKAL DSP+PAQ+
Sbjct: 361 SFLRREVKALVDSPDPAQD 365

BLAST of Clc01G00560 vs. ExPASy TrEMBL
Match: A0A6J1H0Q5 (spermine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459343 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 6.3e-192
Identity = 327/379 (86.28%), Postives = 350/379 (92.35%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           MEADAGRGLGCQKTMDGK SNGI+PKKEIPSCCLKA AS PEL+ KCHSTVVSGWFSE Q
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  LASDNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQL 120
            ASD G K VYFNNPMWPGEAHSLQVESILF+G+SEYQEVVVF+ST+YGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFKSTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVL E++RHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLLA 240
           VSKEFFP+LA GFEDPRVHLH+GDAV+FLRRAP+ KYDAIIVDSSDPV            
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPV------------ 240

Query: 241 ILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYAW 300
             GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLI+DMISICR+IFKGSV+YAW
Sbjct: 241 --GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAW 300

Query: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFALP 360
           GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLE + +++R+LKFYNSEIHSAAFALP
Sbjct: 301 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALP 360

Query: 361 SFLRKEVKALADSPNPAQN 380
           SFLR+EVKAL DSP+PAQ+
Sbjct: 361 SFLRREVKALVDSPDPAQD 365

BLAST of Clc01G00560 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-167
Identity = 288/374 (77.01%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           ME D G GL CQ TMDGK SNG   +K +PSCCLKA A VPE + KCHSTVVSGWFSEP 
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  LAS-DNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQ 120
             S   G K VYFNNPMWPGEAHSL+VE +LFK KS++QEV+VFES TYGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVL EISRH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLL 240
           DVSK+FFP+LA+GF+DPRV LHIGDA EFLR++P GKYDAIIVDSSDPV           
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPV----------- 240

Query: 241 AILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYA 300
              GPA  LVEKPFFET+A+ALKPGGVLCNMAESMWLHTHLIEDMISICR+ FK SV+YA
Sbjct: 241 ---GPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 300

Query: 301 WGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFAL 360
           W SVPTYPSGVIGF+LCSTEGP VDFKNPINPIEKL+GA+ HKR+LKFYNS++H AAFAL
Sbjct: 301 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 359

Query: 361 PSFLRKEVKALADS 374
           P+FLR+EV +L  S
Sbjct: 361 PTFLRREVASLLAS 359

BLAST of Clc01G00560 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-167
Identity = 288/374 (77.01%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           ME D G GL CQ TMDGK SNG   +K +PSCCLKA A VPE + KCHSTVVSGWFSEP 
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  LAS-DNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQ 120
             S   G K VYFNNPMWPGEAHSL+VE +LFK KS++QEV+VFES TYGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVL EISRH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLL 240
           DVSK+FFP+LA+GF+DPRV LHIGDA EFLR++P GKYDAIIVDSSDPV           
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPV----------- 240

Query: 241 AILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYA 300
              GPA  LVEKPFFET+A+ALKPGGVLCNMAESMWLHTHLIEDMISICR+ FK SV+YA
Sbjct: 241 ---GPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 300

Query: 301 WGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFAL 360
           W SVPTYPSGVIGF+LCSTEGP VDFKNPINPIEKL+GA+ HKR+LKFYNS++H AAFAL
Sbjct: 301 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 359

Query: 361 PSFLRKEVKALADS 374
           P+FLR+EV +L  S
Sbjct: 361 PTFLRREVASLLAS 359

BLAST of Clc01G00560 vs. TAIR 10
Match: AT5G53120.3 (spermidine synthase 3 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-167
Identity = 288/374 (77.01%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           ME D G GL CQ TMDGK SNG   +K +PSCCLKA A VPE + KCHSTVVSGWFSEP 
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  LAS-DNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQ 120
             S   G K VYFNNPMWPGEAHSL+VE +LFK KS++QEV+VFES TYGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVL EISRH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLL 240
           DVSK+FFP+LA+GF+DPRV LHIGDA EFLR++P GKYDAIIVDSSDPV           
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPV----------- 240

Query: 241 AILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYA 300
              GPA  LVEKPFFET+A+ALKPGGVLCNMAESMWLHTHLIEDMISICR+ FK SV+YA
Sbjct: 241 ---GPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 300

Query: 301 WGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFAL 360
           W SVPTYPSGVIGF+LCSTEGP VDFKNPINPIEKL+GA+ HKR+LKFYNS++H AAFAL
Sbjct: 301 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 359

Query: 361 PSFLRKEVKALADS 374
           P+FLR+EV +L  S
Sbjct: 361 PTFLRREVASLLAS 359

BLAST of Clc01G00560 vs. TAIR 10
Match: AT5G53120.4 (spermidine synthase 3 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-167
Identity = 288/374 (77.01%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           ME D G GL CQ TMDGK SNG   +K +PSCCLKA A VPE + KCHSTVVSGWFSEP 
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  LAS-DNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQ 120
             S   G K VYFNNPMWPGEAHSL+VE +LFK KS++QEV+VFES TYGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVL EISRH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLL 240
           DVSK+FFP+LA+GF+DPRV LHIGDA EFLR++P GKYDAIIVDSSDPV           
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPV----------- 240

Query: 241 AILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYA 300
              GPA  LVEKPFFET+A+ALKPGGVLCNMAESMWLHTHLIEDMISICR+ FK SV+YA
Sbjct: 241 ---GPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 300

Query: 301 WGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFAL 360
           W SVPTYPSGVIGF+LCSTEGP VDFKNPINPIEKL+GA+ HKR+LKFYNS++H AAFAL
Sbjct: 301 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 359

Query: 361 PSFLRKEVKALADS 374
           P+FLR+EV +L  S
Sbjct: 361 PTFLRREVASLLAS 359

BLAST of Clc01G00560 vs. TAIR 10
Match: AT5G53120.5 (spermidine synthase 3 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-167
Identity = 288/374 (77.01%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKESNGILPKKEIPSCCLKARASVPELEVKCHSTVVSGWFSEPQ 60
           ME D G GL CQ TMDGK SNG   +K +PSCCLKA A VPE + KCHSTVVSGWFSEP 
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  LAS-DNGKKRVYFNNPMWPGEAHSLQVESILFKGKSEYQEVVVFESTTYGKVLVLDGIVQ 120
             S   G K VYFNNPMWPGEAHSL+VE +LFK KS++QEV+VFES TYGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVL EISRH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPDLAIGFEDPRVHLHIGDAVEFLRRAPRGKYDAIIVDSSDPVVYAYSSITKLL 240
           DVSK+FFP+LA+GF+DPRV LHIGDA EFLR++P GKYDAIIVDSSDPV           
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPV----------- 240

Query: 241 AILGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIEDMISICREIFKGSVYYA 300
              GPA  LVEKPFFET+A+ALKPGGVLCNMAESMWLHTHLIEDMISICR+ FK SV+YA
Sbjct: 241 ---GPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 300

Query: 301 WGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLEGAVKHKRDLKFYNSEIHSAAFAL 360
           W SVPTYPSGVIGF+LCSTEGP VDFKNPINPIEKL+GA+ HKR+LKFYNS++H AAFAL
Sbjct: 301 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 359

Query: 361 PSFLRKEVKALADS 374
           P+FLR+EV +L  S
Sbjct: 361 PTFLRREVASLLAS 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004145928.13.6e-20290.77spermine synthase [Cucumis sativus] >KGN49872.1 hypothetical protein Csa_000091 ... [more]
XP_038906466.15.2e-20191.82spermine synthase [Benincasa hispida][more]
XP_008437587.18.4e-19989.45PREDICTED: spermine synthase [Cucumis melo] >XP_008437588.1 PREDICTED: spermine ... [more]
KAA0042556.15.1e-19687.79spermine synthase [Cucumis melo var. makuwa] >TYK05960.1 spermine synthase [Cucu... [more]
KAG7035744.14.4e-19286.54Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q94BN25.7e-16677.01Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1[more]
O821471.5e-12967.90Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1[more]
O486593.0e-12766.56Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1[more]
Q9ZUB32.6e-12665.05Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1[more]
Q965561.7e-12565.11Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KN881.8e-20290.77PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139500 PE=3 S... [more]
A0A1S3AUE04.0e-19989.45spermine synthase OS=Cucumis melo OX=3656 GN=LOC103482957 PE=3 SV=1[more]
A0A5A7TKT12.5e-19687.79Spermine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00... [more]
A0A6J1K5166.3e-19286.02spermine synthase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491249 PE... [more]
A0A6J1H0Q56.3e-19286.28spermine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459343 ... [more]
Match NameE-valueIdentityDescription
AT5G53120.14.0e-16777.01spermidine synthase 3 [more]
AT5G53120.24.0e-16777.01spermidine synthase 3 [more]
AT5G53120.34.0e-16777.01spermidine synthase 3 [more]
AT5G53120.44.0e-16777.01spermidine synthase 3 [more]
AT5G53120.54.0e-16777.01spermidine synthase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 75..122
e-value: 1.4E-19
score: 69.7
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 120..372
e-value: 3.6E-94
score: 316.6
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 125..326
e-value: 2.5E-58
score: 196.6
NoneNo IPR availablePANTHERPTHR11558:SF25SPERMINE SYNTHASEcoord: 1..377
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 167..267
e-value: 1.28743E-6
score: 44.7283
IPR001045Spermidine/spermine synthasesTIGRFAMTIGR00417TIGR00417coord: 79..362
e-value: 1.8E-84
score: 281.1
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 1..377
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 54..368
score: 33.623413
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 46..119
e-value: 4.0E-29
score: 102.8
IPR030373Polyamine biosynthesis domain, conserved sitePROSITEPS01330PABS_1coord: 146..159
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 53..318
score: 92.911705
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 71..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc01G00560.2Clc01G00560.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0004766 spermidine synthase activity
molecular_function GO:0003824 catalytic activity