Cla97C10G193330 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C10G193330
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Trypsin inhibitor 1-like
Location	Cla97Chr10: 21705504 .. 21705794 (-)
RNA-Seq Expression	Cla97C10G193330
Synteny	Cla97C10G193330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAAGAGAATTATTATTATTGTGGCAATAATGGCAATGATCATGATGATGGCTACATCGTCGGCTGCTTTTGCCATCGATGGTGACTCGGTGGATCTCGTCTCTGATGGACGACTTCAAACTGGTCTAGACATCAAGAATGGATGCCCAAGAAAGGCAATGATCATGAAAGGAGGAGCTTTTAAGGAGGAAGGAAGAAGATGTCCCAGAATCTATATGGAATGCAAACGTGACGCAGATTGCTTAGCTGATTGTGTTTGCCTACAGCATGGAATTTGTGGTTAA

mRNA sequence

ATGGAGAGCAAGAGAATTATTATTATTGTGGCAATAATGGCAATGATCATGATGATGGCTACATCGTCGGCTGCTTTTGCCATCGATGGTGACTCGGTGGATCTCGTCTCTGATGGACGACTTCAAACTGGTCTAGACATCAAGAATGGATGCCCAAGAAAGGCAATGATCATGAAAGGAGGAGCTTTTAAGGAGGAAGGAAGAAGATGTCCCAGAATCTATATGGAATGCAAACGTGACGCAGATTGCTTAGCTGATTGTGTTTGCCTACAGCATGGAATTTGTGGTTAA

Coding sequence (CDS)

ATGGAGAGCAAGAGAATTATTATTATTGTGGCAATAATGGCAATGATCATGATGATGGCTACATCGTCGGCTGCTTTTGCCATCGATGGTGACTCGGTGGATCTCGTCTCTGATGGACGACTTCAAACTGGTCTAGACATCAAGAATGGATGCCCAAGAAAGGCAATGATCATGAAAGGAGGAGCTTTTAAGGAGGAAGGAAGAAGATGTCCCAGAATCTATATGGAATGCAAACGTGACGCAGATTGCTTAGCTGATTGTGTTTGCCTACAGCATGGAATTTGTGGTTAA

Protein sequence

MESKRIIIIVAIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEEGRRCPRIYMECKRDADCLADCVCLQHGICG

Homology

[Close]

BLAST of Cla97C10G193330 vs. NCBI nr
Match: KAA0044339.1 (trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibitor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 105.5 bits (262), Expect = 2.5e-19
Identity = 59/99 (59.60%), Postives = 76/99 (76.77%), Query Frame = 0


Query: 1  MESKRIIIIVAIMAMIMMMA--TSSAAFAIDG-DSVDLVSDGRLQTGLDIKNGCPRKAMI 60
          M+SK+ I++VAI+ MI+M+A  +SSA+F +D  DS +LVSDGR     D+ NGCPRK MI
Sbjct: 1  MDSKK-IVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGR-----DMNNGCPRKIMI 60

Query: 61 MKGGAFKEEGRRCPRIYMECKRDADCLADCVCLQHGICG 97
           KGG FKEE R CP+I M+CK+D+DCL DCVCL+ G CG
Sbjct: 61 -KGGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Cla97C10G193330 vs. NCBI nr
Match: KAG6576919.1 (hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 97.4 bits (241), Expect = 6.9e-17
Identity = 50/90 (55.56%), Postives = 66/90 (73.33%), Query Frame = 0


Query: 7   IIIVAIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEE 66
           I+ VA++A++++   SSAA+AID DS+DLV DGR     DI +G PRK  IMK G    E
Sbjct: 59  IVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRK--IMKWGVLNHE 118

Query: 67  GRRCPRIYMECKRDADCLADCVCLQHGICG 97
            R CP+I MECK+D+DCLA+C+CL+HG CG
Sbjct: 119 ERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Cla97C10G193330 vs. NCBI nr
Match: KAG7014944.1 (hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 97.4 bits (241), Expect = 6.9e-17
Identity = 50/90 (55.56%), Postives = 66/90 (73.33%), Query Frame = 0


Query: 7   IIIVAIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEE 66
           I+ VA++A++++   SSAA+AID DS+DLV DGR     DI +G PRK  IMK G    E
Sbjct: 59  IVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRK--IMKWGVLNHE 118

Query: 67  GRRCPRIYMECKRDADCLADCVCLQHGICG 97
            R CP+I MECK+D+DCLA+C+CL+HG CG
Sbjct: 119 ERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Cla97C10G193330 vs. NCBI nr
Match: XP_023552846.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 79.0 bits (193), Expect = 2.6e-11
Identity = 46/79 (58.23%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 8  IIVAIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEEG 67
          + VA++AM M++ATSSAA+AID DS+DLV DGR     DI +G PRK  IMK G    E 
Sbjct: 7  VAVALIAM-MLVATSSAAYAIDDDSMDLVFDGR-----DINHGVPRK--IMKWGVLNHEE 66

Query: 68 RRCPRIYMECKRDADCLAD 87
          R CP+I MECK+D+DCLA+
Sbjct: 67 RVCPKILMECKKDSDCLAE 77

[Close]

BLAST of Cla97C10G193330 vs. NCBI nr
Match: XP_022922566.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 77.4 bits (189), Expect = 7.4e-11
Identity = 43/82 (52.44%), Postives = 58/82 (70.73%), Query Frame = 0


Query: 7  IIIVAIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEE 66
          I+ VA++A++++   SSAA+AID DS+DLV DGR     DI +  PRK  IMK G    E
Sbjct: 6  IVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHRVPRK--IMKWGVLNHE 65

Query: 67 GRRCPRIYMECKRDADCLADCV 89
           R CP+I MECK+D+DCLA+C+
Sbjct: 66 ERVCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Cla97C10G193330 vs. ExPASy Swiss-Prot
Match: P11969 (Trypsin inhibitor 1 OS=Citrullus lanatus OX=3654 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.8e-12
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 67 GRRCPRIYMECKRDADCLADCVCLQHGICG 97
          GRRCPRIYMECKRDADCLADCVCLQHGICG
Sbjct: 1  GRRCPRIYMECKRDADCLADCVCLQHGICG 30

[Close]

BLAST of Cla97C10G193330 vs. ExPASy Swiss-Prot
Match: P26771 (Trypsin inhibitor 1 OS=Lagenaria siceraria OX=3668 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-10
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 68 RRCPRIYMECKRDADCLADCVCLQHGICG 97
          RRCPRIYMECK D+DCLADCVCL+HGICG
Sbjct: 2  RRCPRIYMECKHDSDCLADCVCLEHGICG 30

[Close]

BLAST of Cla97C10G193330 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 8.0e-08
Identity = 22/29 (75.86%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 68 RRCPRIYMECKRDADCLADCVCLQHGICG 97
          R CPRI MECK+D+DCLA+CVCL+HG CG
Sbjct: 1  RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of Cla97C10G193330 vs. ExPASy Swiss-Prot
Match: P10293 (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-07
Identity = 21/31 (67.74%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 66 EGRRCPRIYMECKRDADCLADCVCLQHGICG 97
          E R CP+I MECK+D+DCLA+C+CL+HG CG
Sbjct: 2  EERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of Cla97C10G193330 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-07
Identity = 22/31 (70.97%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 66 EGRRCPRIYMECKRDADCLADCVCLQHGICG 97
          E R CPRI M+CK+D+DCLA+CVCL+HG CG
Sbjct: 2  EERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-19
Identity = 59/99 (59.60%), Postives = 76/99 (76.77%), Query Frame = 0


Query: 1  MESKRIIIIVAIMAMIMMMA--TSSAAFAIDG-DSVDLVSDGRLQTGLDIKNGCPRKAMI 60
          M+SK+ I++VAI+ MI+M+A  +SSA+F +D  DS +LVSDGR     D+ NGCPRK MI
Sbjct: 1  MDSKK-IVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGR-----DMNNGCPRKIMI 60

Query: 61 MKGGAFKEEGRRCPRIYMECKRDADCLADCVCLQHGICG 97
           KGG FKEE R CP+I M+CK+D+DCL DCVCL+ G CG
Sbjct: 61 -KGGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL
Match: A0A0A0KV23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.2e-17
Identity = 52/94 (55.32%), Postives = 68/94 (72.34%), Query Frame = 0


Query: 1  MESKRIIIIVAIMAMIMMMA-TSSAAFAIDG-DSVDLVSDGRLQTGLDIKNGCPRKAMIM 60
          MESK+I+++  +  +I+ +A +SSA+F +D  D + LVSDGR Q   D+ NGCPRK MI 
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMI- 60

Query: 61 KGGAFKEEGRRCPRIYMECKRDADCLADCVCLQH 93
          KGG FKEE   CPRI M+CK D+DCL  CVCL+H
Sbjct: 61 KGGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEH 93

[Close]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL
Match: A0A6J1E4H0 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430533 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.6e-11
Identity = 43/82 (52.44%), Postives = 58/82 (70.73%), Query Frame = 0


Query: 7  IIIVAIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEE 66
          I+ VA++A++++   SSAA+AID DS+DLV DGR     DI +  PRK  IMK G    E
Sbjct: 6  IVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHRVPRK--IMKWGVLNHE 65

Query: 67 GRRCPRIYMECKRDADCLADCV 89
           R CP+I MECK+D+DCLA+C+
Sbjct: 66 ERVCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL
Match: A0A0A7HIA9 (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.4e-09
Identity = 34/83 (40.96%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 14 AMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEEGRRCPRI 73
          A++ MM  +++A +  GD++ L+S+GR QTG DI +          GG + EE R CP+I
Sbjct: 3  ALVAMMLVATSADSNGGDTIHLISNGRAQTGQDINS----------GGVYSEEQRACPKI 62

Query: 74 YMECKRDADCLADCVCLQHGICG 97
             C+RD+DC   CVC  +G CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL
Match: A0A0A7HF94 (TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.5e-09
Identity = 40/86 (46.51%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 11 AIMAMIMMMATSSAAFAIDGDSVDLVSDGRLQTGLDIKNGCPRKAMIMKGGAFKEEGRRC 70
          A++   M++ATS  A   DGD++DL+S+ R QTG DI NG         GG + EE R C
Sbjct: 2  AVLVATMLVATS--ADFNDGDTIDLISNDRAQTGQDI-NG---------GGVYSEEQRAC 61

Query: 71 PRIYMECKRDADCLADCVCLQHGICG 97
          PRI   C+RD+DC   CVC  +G CG
Sbjct: 62 PRILKRCRRDSDCPGACVCQGNGYCG 75

The following BLAST results are available for this feature:

BLAST of Cla97C10G193330 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0044339.1	2.5e-19	59.60	trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibito...	[more]
KAG6576919.1	6.9e-17	55.56	hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014944.1	6.9e-17	55.56	hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023552846.1	2.6e-11	58.23	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]
XP_022922566.1	7.4e-11	52.44	probable polygalacturonase At3g15720 [Cucurbita moschata]	[more]

BLAST of Cla97C10G193330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P11969	1.8e-12	100.00	Trypsin inhibitor 1 OS=Citrullus lanatus OX=3654 PE=1 SV=1	[more]
P26771	1.7e-10	89.66	Trypsin inhibitor 1 OS=Lagenaria siceraria OX=3668 PE=1 SV=2	[more]
P01074	8.0e-08	75.86	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P10293	1.0e-07	67.74	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
P07853	1.0e-07	70.97	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E148	1.2e-19	59.60	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0KV23	1.2e-17	55.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]
A0A6J1E4H0	3.6e-11	52.44	probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305...	[more]
A0A0A7HIA9	3.4e-09	40.96	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]
A0A0A7HF94	7.5e-09	46.51	TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1	[more]

BLAST of Cla97C10G193330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 70..79 score: 75.0 coord: 80..89 score: 72.5
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 68..96 e-value: 2.3E-16 score: 59.5
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 70..89
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 70..96 e-value: 4.40259E-10 score: 48.1427
None	No IPR available	SMART	SM00286	PTI_1	coord: 68..96 e-value: 3.5E-11 score: 53.2
None	No IPR available	GENE3D	4.10.75.20		coord: 54..96 e-value: 2.9E-12 score: 48.2
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 65..96

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C10G193330.1	Cla97C10G193330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity