Cla97C03G057130 (gene) Watermelon (97103) v2.5

Overview
NameCla97C03G057130
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationCla97Chr03: 5810053 .. 5814410 (-)
RNA-Seq ExpressionCla97C03G057130
SyntenyCla97C03G057130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAAAGCTGTAATGACTTCTCTGTCTCCTACATCTCCACCGCCACTTTCCTCCGCCGCCACCACTTTTCCCGTCCCACTTTTTCGAACTAACAAAAAGACCCCACCATTAATCCCTCCAAGAACTAAATTCCTCGTCAATAATGTTTCTCGAGTTTCCTGCAGCACCTCCAATAACGGCAGCTCCCCGGACAAGTTTGATCGGAGAAACCTTCTTCTTGGGCTCGGCGGTCTCTACGGAGCTTCAACTCTCAGCCCAAATCCCTTGGCATCCGCTAATCCAATAGTAACGCCGCCACTCGACACATGTGGCCCCACAGACTTGCCAGCTGGCGCCAAGCCCACCGATTGCTGCCCACCGCCCACCGTCAAAATCAAGGACTTCCGGCCAACCCCACCTCGCCAACTCCGGGTTCGGCCGGCGGCTCATCTAGCTGACAACAATTTCACTGAAAAATACAAGAGAGCCGTCGAGATTATGAAAAGCCTACCGGAAGACGACCCAAGAAGCTTTACACAACAAGCCAATGTTCACTGTGCTTATTGTAATGGCGGGTACGATCAATTGGGTTTTCCGGTTGAGCTTCAAGTTCATAATTCGTGGCTGTTTTTTCCGTTTCATCGTTATTATCTTTATTTTCATGAGAAGATATTGGGAGAGTTAATTGGGGATCCTAATTTCGCTTTGCCGTTTTGGAATTACGATGCTCCGGTGGGAATGAGAATGCCGGCAATTTACGCCGACAAAAGCTCGCCGCTTTATGATGAATTACGCAACCAAAGTCACGTGCCGCCGGCGTTGGTGGACTTGGATTTCAATGACGCAGATGAAGGAGATGAAAATGCTCAAATAAGGAGTAATCTTGGGGTTATGTATCGGCAAGTGGTGTCCGCTAGCCGTACGCCGTTGCTGTTTTTCGGCGGAGCTTATCGGGCCGGCGACGGTCCATCGCCAGGTAATGTTTTTTTCTGGCCACACATTTTCCAAAAGTTTTACTTCCAATATCCATACATTATCTTTCTTTTCTATTCTATTTTATTTGTCTTTTGAGTTTTCAATATATTTATTTCGCTCCAAATTCAAGCTTCTAAACTTTTATTTGTTGTTTGATAAGGATTTTATTTTTTTAAGTGTTGTTTCCTTTAATTATTCTATTATGGTTTTTATTTATTTCAAAGAAAGACCTGAATTTCTAATTAAATTTTTCTTTAAAAAATATCAATTTATTTATACCTGGATTATATCAAATTTAAATTGTGAACTAACAATTGTCTGTATTTAAATCTTGAATTTTTATAAGTCTATTAATTTACACTCTTCATGTAACGTCCAATGTGAGAAAATCTCGTGTAAAAAACTTATAATTATATCAATTAAACTCCTAAACTTTAATAAATGAATCAAGTCAAATCTTAGTCAGAATTTCCAGTGAGAATAAATGCATTTGTTTTGATCTTCCATTTTGTAAAATAGACATTTGAAGAGAAATCCACAAAAATACAATGAATGATTTTTAAAGGTAATTGTTATGAATGATCTAAATTGATTGAAAATTTAGGAGTCTAATTAATTCAAATTATAAGTTTCACACAAGATTGGTCAAATGAAATTTGGTAAAGGATACGAGTTGATAAACTTAAGTTCATTGGTTTAAATTGATACAAACCCAAAGTTTACGTGTATAAATTGATATTTGTTATAAATTTTTTTTCCTCAAAATATGGCTTGATTTTTGAAAACCTGAGTAGAAAAGTGGATTAAAAAAAAAAACGAGAAATTTATAAGTGGAAGTACTGTTGATAACATTAATTTTCTAAAGTTTAAAACTAAAAATAAAATTGTTATCAATGTAAGTGAAAAAGAGTAAATCTACTTAAAAGACATCAAACACAAGTTGATAGATTTTAAATTGGGTTGTTTTCAAATATAGGAAAATGAATCAATATATTTACAAATATAGTAAAATTTTATTGTCTATCAGTGATAGGCCGCGATAGACTATTGTGTCTGTGATAGTAATTTATCATGGTCTATCGTGGTCTATTATAGATAGACTATGATATTTTACTATTATTTGTAAATATTTTCAACACTTTTGTCATTTAAAATAATTTTCCTTTTAAATTTGTGTCTAATAGGTTATTGGACTTTAAAAAATGTTTTATGTCTGATATGCTTCAAATGGTCAAATTTATGTCTAATAGATTTTTTAAATAAATTTTATATCCAATATGGTTTTTAAAAAATCCATAATTTTTAAAATTGAATGATCTTTTAGACATTGAATTTCTTATTAAAAAAAACACTTTAAAATTTTTAATTTTGTATAAAATTAAGTTTAAGTTTTAAAAAATCCAATAATTTAGGAATTATTTGATATCAAATTGAAGGTTAAAAAATATAGTAGTTGTGTAAATTAAAAGTGTGTAAGAGAATAACTTATTCAAAAACTAAGACAATTTTTTTTTTTTTTAAAGTAAGAATTTAGAGACTTGAAATTTTTAAAGTTTATAAATGTATTAGCGACAGGTGATAGTTTGATCTTCACCATTTTTGAATCAATTGAGAATAAAATCGAACCGATTGATTTTATAAAATATGGAACAAACCGATTTAATGAAATTAATTACCGGTTTAAATTGTTATTGGTTTGGCTTGTGTTGGTTTGGTTTCTAATTTGAGGCACTTACAGATATAGTGTTTAAAATATTTAGATATATAACACAATGCACCAAAAAAAAATTGCAAATGGAGCAAAATTTAGATTTTGCTTTTAAAGTTCATACGGTCTATCAGTGATAGAGTCTATTATTGATAAATTTTGCTACATTTGCAACTTTTTAAAAATGGTCCTATACATCTAATTATTATCTCTAAAAGTGCTATTAGATGCAATTATCCTTTTAATTTTAGGTCCAATTGGGCATTTTTATTTTTATTTTTTTTTTCAATTTTTAAAACTTTATTTCGGACATTTTTGTTTTCAATTTAAATTAAAATTTGACATATTTCATTTTTTAAGACTAAAACTGGGTTTTTTTTTTCATTTCTTTTTATTTTTAAAAAATAGTAATTGAATTTTTTTTAAAATAACAAATATACATGAACTATAATAATTAAAATACTAAAATATTAATACAATTCATCATTCACTTGACATAATAAAATACTAATAATACATTATCCATCCCAAATATAAAAGTATCCATTGGGTTCGATTTTTATTGGTTTTGTTTTCAAGAAAACAAACATGAATTAATAATCTTTTTGGTTTCTCCCAATACAAATTAATATATATCCAACTTCACTCACTAAAGCTTAGTACATACGCTTGGATCGACTTTTCAAAATTTTATTGATCTCTCTAAATGTATTGTACAGAACTCAAATTTTTTTTTCTAAAGGACTAATTGAGCAAATATTATAGCGTGTTCGATGTTTATCCTATTGAAACATAAATTCAAAAGTACTTTATATTAACGGCATTACTAATTAGGATTATTAATTCAATATATTAAATAGGAGGGGGAGTTGTGGAAAACATTCCACACGGTCCAGTTCACAGATGGTGCGGCGACAACGAGCAGCCCAACGGCGAGAATATGGGAAACTTCTACTCTGCCGCAAGAGACCCAATCTTCTACGCTCATCACTCCAACGTTGACCGCATGTGGTCCATTTGGAAGACCTTAGGCGGGAAGCGCAAAGACATTCAAAACCCTGATTATCTCAACGCTTCTTTCCTCTTCTACAATGAGAAAGCCGAAGCCGTCCGAGTCTACGTCCGAGACTCTCTCGACACCAAAACCCTCGGCTACGTTTTCCAAGATGTTGACATTCCCTGGCTCAAATCCCGCCCCACTCCTCGTAAGACCCTCAAGTCGAGGAAACCCCGAGCCACCACTGCCAGCATTTCCCCCTTCGACGTTGGCCCAGCGCTTGCTGAGCCAGTCCCCGTGTCGTTCCCACTGACTCTTGATAAAATTGTCAGCATTGAAGTGAAGAGGCCGAGGAAATCGAGGAGTAAGAAGGAGAAAGAGGAGGAGGAGGAGGTGTTGGTGATTGATGGCATTGAATTCAATAGTAATACAGCTGTAAAGTTCGATGTTTTGATTAATGACGAGGATGATAGGGAGATTAGAGCTGATAATACGGAGTTTGCTGGGAGCTTTGTGAATATACCACATTTGAGAGGGAAGGGTAAGACGGAAAAGACTTGCTTTAGATTAGGGATAAGTGAATTACTTGAGGATTTGGATGCGGATGATGACGACAGTGTGATTGTCACTTTAGTGCCCAAATTTGGATATGATAAACTTATTGTTGGCAAGGTTAAGATTGAATTAGAAGAATAG

mRNA sequence

ATGATCAAAGCTGTAATGACTTCTCTGTCTCCTACATCTCCACCGCCACTTTCCTCCGCCGCCACCACTTTTCCCGTCCCACTTTTTCGAACTAACAAAAAGACCCCACCATTAATCCCTCCAAGAACTAAATTCCTCGTCAATAATGTTTCTCGAGTTTCCTGCAGCACCTCCAATAACGGCAGCTCCCCGGACAAGTTTGATCGGAGAAACCTTCTTCTTGGGCTCGGCGGTCTCTACGGAGCTTCAACTCTCAGCCCAAATCCCTTGGCATCCGCTAATCCAATAGTAACGCCGCCACTCGACACATGTGGCCCCACAGACTTGCCAGCTGGCGCCAAGCCCACCGATTGCTGCCCACCGCCCACCGTCAAAATCAAGGACTTCCGGCCAACCCCACCTCGCCAACTCCGGGTTCGGCCGGCGGCTCATCTAGCTGACAACAATTTCACTGAAAAATACAAGAGAGCCGTCGAGATTATGAAAAGCCTACCGGAAGACGACCCAAGAAGCTTTACACAACAAGCCAATGTTCACTGTGCTTATTGTAATGGCGGGTACGATCAATTGGGTTTTCCGGTTGAGCTTCAAGTTCATAATTCGTGGCTGTTTTTTCCGTTTCATCGTTATTATCTTTATTTTCATGAGAAGATATTGGGAGAGTTAATTGGGGATCCTAATTTCGCTTTGCCGTTTTGGAATTACGATGCTCCGGTGGGAATGAGAATGCCGGCAATTTACGCCGACAAAAGCTCGCCGCTTTATGATGAATTACGCAACCAAAGTCACGTGCCGCCGGCGTTGGTGGACTTGGATTTCAATGACGCAGATGAAGGAGATGAAAATGCTCAAATAAGGAGTAATCTTGGGGTTATGTATCGGCAAGTGGTGTCCGCTAGCCGTACGCCGTTGCTGTTTTTCGGCGGAGCTTATCGGGCCGGCGACGGTCCATCGCCAGGAGGGGGAGTTGTGGAAAACATTCCACACGGTCCAGTTCACAGATGGTGCGGCGACAACGAGCAGCCCAACGGCGAGAATATGGGAAACTTCTACTCTGCCGCAAGAGACCCAATCTTCTACGCTCATCACTCCAACGTTGACCGCATGTGGTCCATTTGGAAGACCTTAGGCGGGAAGCGCAAAGACATTCAAAACCCTGATTATCTCAACGCTTCTTTCCTCTTCTACAATGAGAAAGCCGAAGCCGTCCGAGTCTACGTCCGAGACTCTCTCGACACCAAAACCCTCGGCTACGTTTTCCAAGATGTTGACATTCCCTGGCTCAAATCCCGCCCCACTCCTCGTAAGACCCTCAAGTCGAGGAAACCCCGAGCCACCACTGCCAGCATTTCCCCCTTCGACGTTGGCCCAGCGCTTGCTGAGCCAGTCCCCGTGTCGTTCCCACTGACTCTTGATAAAATTGTCAGCATTGAAGTGAAGAGGCCGAGGAAATCGAGGAGTAAGAAGGAGAAAGAGGAGGAGGAGGAGGTGTTGGTGATTGATGGCATTGAATTCAATAGTAATACAGCTGTAAAGTTCGATGTTTTGATTAATGACGAGGATGATAGGGAGATTAGAGCTGATAATACGGAGTTTGCTGGGAGCTTTGTGAATATACCACATTTGAGAGGGAAGGGTAAGACGGAAAAGACTTGCTTTAGATTAGGGATAAGTGAATTACTTGAGGATTTGGATGCGGATGATGACGACAGTGTGATTGTCACTTTAGTGCCCAAATTTGGATATGATAAACTTATTGTTGGCAAGGTTAAGATTGAATTAGAAGAATAG

Coding sequence (CDS)

ATGATCAAAGCTGTAATGACTTCTCTGTCTCCTACATCTCCACCGCCACTTTCCTCCGCCGCCACCACTTTTCCCGTCCCACTTTTTCGAACTAACAAAAAGACCCCACCATTAATCCCTCCAAGAACTAAATTCCTCGTCAATAATGTTTCTCGAGTTTCCTGCAGCACCTCCAATAACGGCAGCTCCCCGGACAAGTTTGATCGGAGAAACCTTCTTCTTGGGCTCGGCGGTCTCTACGGAGCTTCAACTCTCAGCCCAAATCCCTTGGCATCCGCTAATCCAATAGTAACGCCGCCACTCGACACATGTGGCCCCACAGACTTGCCAGCTGGCGCCAAGCCCACCGATTGCTGCCCACCGCCCACCGTCAAAATCAAGGACTTCCGGCCAACCCCACCTCGCCAACTCCGGGTTCGGCCGGCGGCTCATCTAGCTGACAACAATTTCACTGAAAAATACAAGAGAGCCGTCGAGATTATGAAAAGCCTACCGGAAGACGACCCAAGAAGCTTTACACAACAAGCCAATGTTCACTGTGCTTATTGTAATGGCGGGTACGATCAATTGGGTTTTCCGGTTGAGCTTCAAGTTCATAATTCGTGGCTGTTTTTTCCGTTTCATCGTTATTATCTTTATTTTCATGAGAAGATATTGGGAGAGTTAATTGGGGATCCTAATTTCGCTTTGCCGTTTTGGAATTACGATGCTCCGGTGGGAATGAGAATGCCGGCAATTTACGCCGACAAAAGCTCGCCGCTTTATGATGAATTACGCAACCAAAGTCACGTGCCGCCGGCGTTGGTGGACTTGGATTTCAATGACGCAGATGAAGGAGATGAAAATGCTCAAATAAGGAGTAATCTTGGGGTTATGTATCGGCAAGTGGTGTCCGCTAGCCGTACGCCGTTGCTGTTTTTCGGCGGAGCTTATCGGGCCGGCGACGGTCCATCGCCAGGAGGGGGAGTTGTGGAAAACATTCCACACGGTCCAGTTCACAGATGGTGCGGCGACAACGAGCAGCCCAACGGCGAGAATATGGGAAACTTCTACTCTGCCGCAAGAGACCCAATCTTCTACGCTCATCACTCCAACGTTGACCGCATGTGGTCCATTTGGAAGACCTTAGGCGGGAAGCGCAAAGACATTCAAAACCCTGATTATCTCAACGCTTCTTTCCTCTTCTACAATGAGAAAGCCGAAGCCGTCCGAGTCTACGTCCGAGACTCTCTCGACACCAAAACCCTCGGCTACGTTTTCCAAGATGTTGACATTCCCTGGCTCAAATCCCGCCCCACTCCTCGTAAGACCCTCAAGTCGAGGAAACCCCGAGCCACCACTGCCAGCATTTCCCCCTTCGACGTTGGCCCAGCGCTTGCTGAGCCAGTCCCCGTGTCGTTCCCACTGACTCTTGATAAAATTGTCAGCATTGAAGTGAAGAGGCCGAGGAAATCGAGGAGTAAGAAGGAGAAAGAGGAGGAGGAGGAGGTGTTGGTGATTGATGGCATTGAATTCAATAGTAATACAGCTGTAAAGTTCGATGTTTTGATTAATGACGAGGATGATAGGGAGATTAGAGCTGATAATACGGAGTTTGCTGGGAGCTTTGTGAATATACCACATTTGAGAGGGAAGGGTAAGACGGAAAAGACTTGCTTTAGATTAGGGATAAGTGAATTACTTGAGGATTTGGATGCGGATGATGACGACAGTGTGATTGTCACTTTAGTGCCCAAATTTGGATATGATAAACTTATTGTTGGCAAGGTTAAGATTGAATTAGAAGAATAG

Protein sequence

MIKAVMTSLSPTSPPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPDKFDRRNLLLGLGGLYGASTLSPNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQLGFPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEGDENAQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIELEE
Homology
BLAST of Cla97C03G057130 vs. NCBI nr
Match: APO14262.1 (polyphenol oxidase 3 [Luffa aegyptiaca])

HSP 1 Score: 818.9 bits (2114), Expect = 2.8e-233
Identity = 403/598 (67.39%), Postives = 474/598 (79.26%), Query Frame = 0

Query: 12  TSPPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPDKFDRRN 71
           TSPP LSSAATT     F  N K PP    RTK+  +   R+ CS SN G   +  DRR+
Sbjct: 3   TSPPALSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQ--NHIDRRD 62

Query: 72  LLLGLGGLYGASTLSPNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFRP 131
           +L+GLGGLYGASTLS   LA A P+ TP +  CG  DLP GA P +CCPPPT  + DFRP
Sbjct: 63  ILIGLGGLYGASTLSQGSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRP 122

Query: 132 TPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQLG 191
             P +LRVRPAAHLAD N+ EKYKRAVE+MK+LP+DDPRSF QQANVHCAYCNG YDQ+G
Sbjct: 123 QAPGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVG 182

Query: 192 FPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADKS 251
           FPVE+QVHNSWLFFPFHRYYL+FHEKILGELIGDP+FALPFWNYDAP GM+MPA+YAD+ 
Sbjct: 183 FPVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEK 242

Query: 252 SPLYDELRNQSHVPPALVDLDFNDADE--GDENAQIRSNLGVMYRQVVSASRTPLLFFGG 311
           S LYDELRN++H+PP LVDLDF   D   GDE AQIRSNL +MYRQ+VS SRTP LFFG 
Sbjct: 243 SSLYDELRNRNHLPPTLVDLDFGGVDPTIGDE-AQIRSNLSIMYRQMVSNSRTPSLFFGN 302

Query: 312 AYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRM 371
           AY AGD PSPGGG+VENIPHGPVH WCGDN +PN ENMGNFYSAARDPIFYAHHSN+DR+
Sbjct: 303 AYLAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRL 362

Query: 372 WSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLK 431
           WS+WKTLGG+R+D+QN D+LNASF+FY+EKA+AVRV VRD LD++ LGYV+QDV +PWL+
Sbjct: 363 WSVWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLE 422

Query: 432 SRPTPRKTLKSRKPRATTASISPFDVGPALA-----------EPVPVSFPLTLDKIVSIE 491
           SRPTPR+ L+++K  A      PF VGPALA            P  + FP+TL+  +S+E
Sbjct: 423 SRPTPRRALRAQKAVAHL----PFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISME 482

Query: 492 VKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVN 551
           VKRP++SRSKKEK+EEEE++VI GIEF++N A+KFDV INDEDD+EIR DNTEFAGSFVN
Sbjct: 483 VKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVN 542

Query: 552 IPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIELEE 597
           +PH  G  K  KT  RLGI+ELLEDLDADDDDSV VTLVP+ G   + +  +KIE ++
Sbjct: 543 VPHKHGHKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEFDK 593

BLAST of Cla97C03G057130 vs. NCBI nr
Match: XP_038903169.1 (polyphenol oxidase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 788.9 bits (2036), Expect = 3.1e-224
Identity = 385/567 (67.90%), Postives = 452/567 (79.72%), Query Frame = 0

Query: 41  PRTKFLVNNVSR--VSCSTSNN---GSSPDKFDRRNLLLGLGGLYGASTLSPNP-LASAN 100
           P+TK L NN+S   +SC+T N+    +   K DRR++L+GL GLYGASTL  N   A A 
Sbjct: 22  PKTKTLPNNISPRIISCNTVNHNDQNTPKPKIDRRDILVGLSGLYGASTLYKNSNFALAA 81

Query: 101 PIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKY 160
           PI TP +  CGP DLP GA  T+CCPPP  +I DF+P    +LRVRPAAHL D N+ EKY
Sbjct: 82  PIQTPNISKCGPADLPPGADSTNCCPPPATRILDFQPPAQEKLRVRPAAHLVDKNYIEKY 141

Query: 161 KRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQLGFPVELQVHNSWLFFPFHRYYLYF 220
           K+AVE+MK+LPE+DPRSFTQQANVHCAYCNG YDQ+GFPVE+QVHNSWLFFPFHR YLYF
Sbjct: 142 KKAVELMKALPENDPRSFTQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRLYLYF 201

Query: 221 HEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFN 280
           HEKILGELIGDPNFALPFWNYDAP GMRMP +YA++ S LYDELRN++HVPP LVDLDF 
Sbjct: 202 HEKILGELIGDPNFALPFWNYDAPGGMRMPEMYAEEKSSLYDELRNENHVPPRLVDLDFG 261

Query: 281 DADEG-DENAQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVH 340
             D    ++AQIRSNL +MYRQ+VS SRTP LFFG AYRAGD PSPGGG +ENIPHGPVH
Sbjct: 262 RVDPNIGDDAQIRSNLSIMYRQMVSNSRTPSLFFGSAYRAGDEPSPGGGSIENIPHGPVH 321

Query: 341 RWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASF 400
            WCGDN +PNGENMGNFYSAARDPIFYAHHSN+DR+WS+WKTLGG+R+D+QNP++LN SF
Sbjct: 322 VWCGDNNEPNGENMGNFYSAARDPIFYAHHSNIDRLWSVWKTLGGRRQDLQNPEWLNTSF 381

Query: 401 LFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPF 460
           +FYNEKAEAVRV VR SLD+K LGYV+QDVD+PWL++RPTPR   +++K  A    I PF
Sbjct: 382 IFYNEKAEAVRVRVRQSLDSKILGYVYQDVDVPWLQTRPTPR---RAQKTVANNNIILPF 441

Query: 461 DVGPALA--------EPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEF 520
            +GPALA         P  + FP+T+   VS+EVKRP+KSRSKKEKEEEEE+LVI GI F
Sbjct: 442 SIGPALAAGPSSSRVRPANIKFPITVKSAVSVEVKRPKKSRSKKEKEEEEEILVIYGIGF 501

Query: 521 NSNTAVKFDVLINDEDDREIRADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLD 580
           + N  +KFDV INDEDD+EIR DNTEFAGSFVN+PH  G  KT KT  RLGI+E+LEDL+
Sbjct: 502 DPNQGIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKLGHKKTVKTGLRLGITEILEDLE 561

Query: 581 ADDDDSVIVTLVPKFGYDKLIVGKVKI 593
           ADDDDSV VT+VP+ G     +  +KI
Sbjct: 562 ADDDDSVRVTIVPRLGAGLFSIDGIKI 585

BLAST of Cla97C03G057130 vs. NCBI nr
Match: XP_008442274.1 (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056968.1 polyphenol oxidase [Cucumis melo var. makuwa] >TYK26394.1 polyphenol oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 779.2 bits (2011), Expect = 2.5e-221
Identity = 373/558 (66.85%), Postives = 453/558 (81.18%), Query Frame = 0

Query: 47  VNNVSRVSCSTSNN---GSSPDKFDRRNLLLGLGGLYGASTLSPNP-LASANPIVTPPLD 106
           ++  SR+SC  SN+    +S  K DRR++L+GL GLYG+STLS +P  A A PI TP + 
Sbjct: 22  ISITSRISCKASNHNDQNTSNPKIDRRDILIGLTGLYGSSTLSQSPHFALAAPIQTPNIS 81

Query: 107 TCGPTDLPAGAKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMK 166
            CGP DLP GA PT+CCPPP   I DF+P PP +LRVRPAAHLAD N+ EKYK+A+E+MK
Sbjct: 82  KCGPPDLPPGADPTNCCPPPATTILDFQPPPPTKLRVRPAAHLADQNYIEKYKKAIELMK 141

Query: 167 SLPEDDPRSFTQQANVHCAYCNGGYDQLGFPVELQVHNSWLFFPFHRYYLYFHEKILGEL 226
           +LPEDDPRSFTQQANVHCAYCNG YDQ+GFPVE+QVHNSWLFFPFHR+YLYFHEKILGEL
Sbjct: 142 ALPEDDPRSFTQQANVHCAYCNGAYDQIGFPVEVQVHNSWLFFPFHRFYLYFHEKILGEL 201

Query: 227 IGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEGD-- 286
           IGD +FALPFWNYDAP GM+MP +YA++ S LYDELRN++H+PP LVDLDF   D+ +  
Sbjct: 202 IGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYDELRNENHLPPRLVDLDFGGVDDPNIG 261

Query: 287 ENAQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNE 346
           ++AQIRSNL +MYRQ+VS SRTP LFFG AY  GD PSPGGG VENIPHGPVH WCGDN+
Sbjct: 262 DDAQIRSNLSIMYRQMVSGSRTPSLFFGSAYVEGDEPSPGGGSVENIPHGPVHVWCGDND 321

Query: 347 QPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKA 406
           QPN ENMGNFYSAARDPIF+AHHSNVDR+WS+WKTLGG+RKD+QNP++LNASF+FYNEKA
Sbjct: 322 QPNFENMGNFYSAARDPIFFAHHSNVDRLWSVWKTLGGRRKDLQNPEWLNASFIFYNEKA 381

Query: 407 EAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALA 466
           + VRV+VRD LD+K+LGY +Q VD+PWL++RPTPR   +++K        S    GP+ +
Sbjct: 382 QPVRVHVRDCLDSKSLGYTYQPVDLPWLQTRPTPR---RAQKTTVAFGIASALAAGPSSS 441

Query: 467 --EPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLIN 526
             +P  + FP+T+   VS+EVKRP+KSRSKKEKEEEEEVLVI GI F+ N  +KFDV IN
Sbjct: 442 RVKPANIKFPITVKSPVSVEVKRPKKSRSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFIN 501

Query: 527 DEDDREIRADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVP 586
           DEDD+EI+ DNTEFAGSFVN+PH +   K  KT  R+GI+E+LEDL+AD+DDS+IVT+VP
Sbjct: 502 DEDDKEIKPDNTEFAGSFVNVPHKQAHKKKVKTGLRVGITEVLEDLEADNDDSIIVTIVP 561

Query: 587 KFGYDKLIVGKVKIELEE 597
           K G     +G +KIEL+E
Sbjct: 562 KIGAGLFSIGGLKIELDE 576

BLAST of Cla97C03G057130 vs. NCBI nr
Match: XP_023001713.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 768.8 bits (1984), Expect = 3.3e-218
Identity = 376/584 (64.38%), Postives = 457/584 (78.25%), Query Frame = 0

Query: 14  PPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPDKFDRRNLL 73
           PPP  S AT      FR + +     PP +K+L NN +RV CS +N      K DRR++L
Sbjct: 5   PPPALSTAT------FRPSSR-----PPTSKYLPNNAARVLCSAANG-----KIDRRDVL 64

Query: 74  LGL--GGLYGASTLS-PNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFR 133
           +G+  GG+YGAS  +  +  A A P+ TP +  CGP DLP G+ PT+CCPPP+  I DFR
Sbjct: 65  IGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFR 124

Query: 134 PTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQL 193
           P  P +LRVRPAAHLAD ++ +KYKRAVE+MK LP DDPRSF QQANVHCAYCNGGYDQ+
Sbjct: 125 PPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQV 184

Query: 194 GFPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADK 253
           GFPVE+QVHNSWLFFPFHRYYLYF+EKILGELIGDP FALPFWNYDAP GMRMPA+YAD 
Sbjct: 185 GFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADH 244

Query: 254 SSPLYDELRNQSHVPPALVDLDFNDADEG-DENAQIRSNLGVMYRQVVSASRTPLLFFGG 313
           +S LYD+LRNQ+H+PP L+DLDF+  D    +NAQIRSNL +MYRQ+VS SRTP LFFG 
Sbjct: 245 NSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGS 304

Query: 314 AYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRM 373
           AY AGD PSPGGG VENIPH  VH WCGDN +PN ENMGNFYSAARDPIFY+HHSNVDRM
Sbjct: 305 AYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRM 364

Query: 374 WSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLK 433
           WS+WKTLGG+R+D+Q P++L+ASF+FYNEKA+AVRV VRD LDTK LGY++QDV++PWL+
Sbjct: 365 WSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLE 424

Query: 434 SRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVSFPLTLDKIVSIEVKRPRKSRSKK 493
           +RPTPR+ L+++K      S+   +V     +P  +  P+TL   +S EVKR +KSR +K
Sbjct: 425 TRPTPRRALRAQK------SVDS-NVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRK 484

Query: 494 EKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVNIPHLRGKGKTE 553
           EK+EEEE+LVI GIEF+ NT++K D+ INDEDDREIR DNTEFAGSFVN+PH  G  K  
Sbjct: 485 EKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKV 544

Query: 554 KTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIE 594
           KT  RLGISE+LEDLDADDDDS+IVT++P+ G   + + ++KIE
Sbjct: 545 KTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIE 565

BLAST of Cla97C03G057130 vs. NCBI nr
Match: KAG6584007.1 (hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 760.4 bits (1962), Expect = 1.2e-215
Identity = 374/584 (64.04%), Postives = 452/584 (77.40%), Query Frame = 0

Query: 14  PPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPDKFDRRNLL 73
           PPP  S A   P    RT           +K+L  N +R+ CS ++      K DRR++L
Sbjct: 5   PPPALSTAAFRPSSSLRT-----------SKYLPINAARILCSAADG-----KIDRRDVL 64

Query: 74  LGL--GGLYGASTLS-PNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFR 133
           +G+  GGLYGAS  +  +  A A P+ TP +  CGP DLP GA PT+CCPPPT  I DF 
Sbjct: 65  IGIGAGGLYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFL 124

Query: 134 PTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQL 193
           P  P +LRVRPAAHLAD ++ +KYKRAVE+MK LPEDDPRSF QQANVHCAYCNGGYDQ+
Sbjct: 125 PPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQV 184

Query: 194 GFPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADK 253
           GFPVE+QVHNSWLFFPFHRYYLYF+EKILGELI DP FALPFWNYDAP GM+MPA+YAD+
Sbjct: 185 GFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIEDPTFALPFWNYDAPGGMKMPAMYADQ 244

Query: 254 SSPLYDELRNQSHVPPALVDLDFNDADEG-DENAQIRSNLGVMYRQVVSASRTPLLFFGG 313
           +S LYD+LRNQ+H+PP  +DLDF+  D    +NAQIRSNL +MYRQ+VS SRTP LFFG 
Sbjct: 245 NSSLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGS 304

Query: 314 AYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRM 373
           AY AGD PSPGGG VENIPH  VH WCGDN +PN ENMGNFYSAARDPIFY+HHSN+DRM
Sbjct: 305 AYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRM 364

Query: 374 WSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLK 433
           WS+WKTLGG+R+D+Q P++L+ASF+FYNEKA+AVRV VRD LDTKTLGY++QDV++PWL+
Sbjct: 365 WSVWKTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKTLGYIYQDVELPWLE 424

Query: 434 SRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVSFPLTLDKIVSIEVKRPRKSRSKK 493
           +RPTPR+ L+++K      S+   +V     +P  + FP+T+   VS EVKR +KSRSKK
Sbjct: 425 TRPTPRRALRAQK------SVDS-NVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKK 484

Query: 494 EKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVNIPHLRGKGKTE 553
           EKEEEEE+LVI GIEF+ NTA+K DV INDEDD EIR DNTEFAGSFVN+PH  G  K  
Sbjct: 485 EKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKV 544

Query: 554 KTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIE 594
           KT  RLGISE+LEDL+ADDDDS+IVT++P+ G   + + ++ IE
Sbjct: 545 KTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITIE 565

BLAST of Cla97C03G057130 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 6.6e-193
Identity = 339/596 (56.88%), Postives = 422/596 (70.81%), Query Frame = 0

Query: 6   MTSLSP--TSPPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSN---N 65
           MTSLSP   + P + + AT    P  + N +   L  P+  F      +VSC  +N   N
Sbjct: 1   MTSLSPPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKRSF----ARKVSCKATNNDQN 60

Query: 66  GSSPDKFDRRNLLLGLGGLYGASTLSPNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCP 125
             +  K DRRN+LLGLGGLYG + +  +P A A PI  P +  CGP DLP GA PT+CCP
Sbjct: 61  DQAQSKLDRRNVLLGLGGLYGVAGMGTDPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCP 120

Query: 126 PPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHC 185
           PP+ KI DF+   P +LR+RP AH  D  + +KY +A+E+MK+LP+DDPRSF QQA VHC
Sbjct: 121 PPSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHC 180

Query: 186 AYCNGGYDQLGFP-VELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPV 245
           AYC+G YDQ+GFP +ELQ+HNSWLFFPFHRYYLYF EKILG+LI DP FALPFWN+D+P 
Sbjct: 181 AYCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPA 240

Query: 246 GMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEG-DENAQIRSNLGVMYRQVVS 305
           GM +PAIYAD  SPLYD+LR+ +H PP LVDLD+N  ++   +   I +NL +MYRQ+VS
Sbjct: 241 GMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVS 300

Query: 306 ASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPI 365
            S+   LFFG  YRAGD P PGGG +E  PH PVH W GDN QPN E+MGNFYSA RDPI
Sbjct: 301 NSKNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPI 360

Query: 366 FYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGY 425
           F+AHHSNVDRMWSIWKTLGGKR D+ + D+L++ FLFYNE AE VRV VRD L+TK LGY
Sbjct: 361 FFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGY 420

Query: 426 VFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVSFPLTLDKIVSIE 485
           V+QDVDIPWL S+PTPR+   +    A    ++   V  +        FP++L   +S  
Sbjct: 421 VYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTV 480

Query: 486 VKRPR-KSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFV 545
           VKRP+ K RSKK KE+EEE+LVI+GIEF+ + AVKFDV +ND DD     D TEFAGSFV
Sbjct: 481 VKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGSFV 540

Query: 546 NIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIE 594
           ++PH     K   T  RLG+++LLE+++A+DDDSV+VTLVPKFG  K  +G +KIE
Sbjct: 541 SVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGAVK--IGGIKIE 590

BLAST of Cla97C03G057130 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 3.0e-177
Identity = 324/566 (57.24%), Postives = 403/566 (71.20%), Query Frame = 0

Query: 52  RVSCSTSNNGSSPDKFD--------RRNLLLGLGGLYGASTLSPNPLASANPIVTPPLDT 111
           +V+CS++NN ++P +          RRN+L+GLGG+YG  TL+ NP A A+PI  P L  
Sbjct: 45  QVTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGGIYG--TLATNPSALASPISPPDLSK 104

Query: 112 C-GPTDLPAGAKP--TDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEI 171
           C  P+DLP+G  P   +CCPP + KI DF+    + LRVR AAHL DN F EKYK+A E+
Sbjct: 105 CVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQPLRVRQAAHLVDNEFLEKYKKATEL 164

Query: 172 MKSLPEDDPRSFTQQANVHCAYCNGGYDQLGFP-VELQVHNSWLFFPFHRYYLYFHEKIL 231
           MK+LP +DPR+FTQQAN+HCAYC+G Y Q+GFP ++LQVH SWLFFPFHR+YLYF+E+IL
Sbjct: 165 MKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDLKLQVHGSWLFFPFHRWYLYFYERIL 224

Query: 232 GELIGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFND-ADE 291
           G LI DP FALPFWNYDAP GM++P IYADK+SPLYDELRN SH PP L+DL+F D   +
Sbjct: 225 GSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNASHQPPTLIDLNFCDIGSD 284

Query: 292 GDENAQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGD 351
            D N  I++NL +MYRQV S  +T  LF G  YRAGD    G G +EN+PH PVH W GD
Sbjct: 285 IDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAGDAEPQGAGSIENVPHAPVHTWTGD 344

Query: 352 NEQPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNE 411
           N Q N E+MG FYSAARDPIFY+HHSNVDR+W IWKTLGGK+ D  + D+L + FLFY+E
Sbjct: 345 NTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDE 404

Query: 412 KAEAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPA 471
               VRV V+DSLD   LGY +QDV IPW K++P PR+T K +K      +       P 
Sbjct: 405 NKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRRT-KVQKLVEVEVNDGNLRKSPT 464

Query: 472 L---AEPVP---VSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVK 531
           +    +  P   V+FPL L+  VS  VKRP+K RSKKEKEEEEEVLVI+GIEF  N A+K
Sbjct: 465 IFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIK 524

Query: 532 FDVLINDEDDREIRADNTEFAGSFVNIPHLRGKGKTEK--TCFRLGISELLEDLDADDDD 591
           FDV INDEDD+ + A NTEFAGSFVNIPH     K +K  T  RLGI++LLEDL  + DD
Sbjct: 525 FDVYINDEDDK-VGAGNTEFAGSFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDD 584

Query: 592 SVIVTLVPKFGYDKLIVGKVKIELEE 597
           +++VTLVPK G  ++ +  V+I  E+
Sbjct: 585 NIVVTLVPKCGSGQVKINNVEIVFED 606

BLAST of Cla97C03G057130 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.2e-162
Identity = 307/608 (50.49%), Postives = 395/608 (64.97%), Query Frame = 0

Query: 7   TSLSPTSPPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPD- 66
           T+ SPT+    SS  +T     F  NK    L   ++K  V   S +SC  +NN +S + 
Sbjct: 3   TAPSPTTMGTYSSLISTNSFSTFLPNKSQLSL-SGKSKHYVARRSSISCKATNNNNSNNQ 62

Query: 67  ------------KFDRRNLLLGLGGLYGASTLSPNPLASANPIVTPPLDTCGPTDL-PAG 126
                       K DRRN+L+GLGGLYGA+TL   P A A+PI  P L TC P ++ P G
Sbjct: 63  NEQQEESSRLLGKLDRRNILIGLGGLYGATTLDRKPFAFADPIAPPDLTTCKPAEITPGG 122

Query: 127 AKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSF 186
           ++   CCPP T KIK F+P     LR  PAAH   + +  K+K+A   M++LP+DDPRS 
Sbjct: 123 SETVPCCPPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSM 182

Query: 187 TQQANVHCAYCNGGYDQLGF-PVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALP 246
            QQA VHCAYCNG Y Q+GF   ++QVH SWLFFPFHR YLYF+E+ILG+LI DP FALP
Sbjct: 183 VQQAKVHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALP 242

Query: 247 FWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEG-DENAQIRSNLG 306
           +WN+D+PVG  +P IY D SSPLYD+ RN  H PP LVDL +   D+  DE  +I  NL 
Sbjct: 243 YWNWDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLA 302

Query: 307 VMYRQVVSASRTPLLFFGGAYRAGD---GPSPGGGVVENIPHGPVHRWCGDNEQPNGENM 366
           +MYRQ+VS ++TP LFFG AYRAG+   G  P  G +EN+PH  +H W GD  Q + E+M
Sbjct: 303 IMYRQMVSGAKTPDLFFGHAYRAGNLNTGKYP--GTIENMPHNNIHIWVGDPSQTHQEDM 362

Query: 367 GNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYV 426
           GNFYSA RDP+FYAHH+NVDRMW+IWKTLGGKRKDI + D+L+A FLFY+E AE VRV V
Sbjct: 363 GNFYSAGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKV 422

Query: 427 RDSLD-TKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVS 486
           RDSL+  K L Y ++ V +PWL ++PT RKT    K +     ++               
Sbjct: 423 RDSLEPEKQLRYNYEPVSLPWLFTKPTARKTKNKTKAKVAATQLTS-------------K 482

Query: 487 FPLTLDKIVSIEVKRPR-KSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREI 546
           FP TL ++ ++EV RP+ + RSKKEK +EEE+L+I  IEF    AVKFDV IND+ +   
Sbjct: 483 FPATLVEVTTVEVARPKPRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFINDDAESLS 542

Query: 547 RADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKL 594
           R D +EFAGSFV++P  +      KT  +LGI++LLEDL A+DD SV+VTLVP+     +
Sbjct: 543 RRDKSEFAGSFVHVPQGKTTKAKTKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPI 594

BLAST of Cla97C03G057130 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 5.1e-161
Identity = 310/582 (53.26%), Postives = 384/582 (65.98%), Query Frame = 0

Query: 35  TPPLIPPRTKFLVNNVSRVSCSTSN-NGSSPD------KFDRRNLLLGLGGLYGASTLSP 94
           TP   P  TK    ++ RVSC  SN N   P+        DRRN+L+GLGGLYGAS + P
Sbjct: 13  TPYSFPYHTKPSRVSLRRVSCKASNDNKDKPNDQEKTFSIDRRNMLIGLGGLYGASNVFP 72

Query: 95  -NPLASANPIVTPPL-DTCGPTDLPAGAKPTDCCPPPTVKIKDFR-PTPPRQLRVRPAAH 154
            N    A PI  P L D+C P +  A      CCPP      DF+ P+ P +LR+RPAAH
Sbjct: 73  SNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCCPPDVKDPIDFQMPSNPSRLRIRPAAH 132

Query: 155 LADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQLGFP-VELQVHNSWL 214
           LAD  + EKYK+A+  MK+LP++DPRSF QQAN+HCAYCNG YDQ+GFP V +QVH+SWL
Sbjct: 133 LADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQVGFPDVNIQVHHSWL 192

Query: 215 FFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSH 274
           F PFHR+YLYF+E+ILG LI DP FA+PFWN+DAP GM MP ++ D +SPLYD  RN +H
Sbjct: 193 FLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPNSPLYDAKRNPAH 252

Query: 275 VPPALVDLDFNDADEGDEN-AQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGG- 334
            P  +VDLDF+  +    N  QI +NL +MY+QVV A +   LF G   +AG  P   G 
Sbjct: 253 FPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVVRAKKA-RLFHGRPLQAGSFPDESGD 312

Query: 335 GVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRK 394
           G +E  PHG +H W GD  Q N ENMGNFYSA RDP+FYAHH+NVDRMW IWK   G RK
Sbjct: 313 GSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVDRMWYIWKNSLG-RK 372

Query: 395 DIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKT---- 454
           D +  D+LNA FL ++E A+ VRVYVRD+LD + LGY +Q+VDIPW+ S+P PRK     
Sbjct: 373 DYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEVDIPWINSKPKPRKANPWP 432

Query: 455 --LKSRKPRATTASISPFDVGPALAEPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEE 514
             L+S K   TT  I+               FPLTLD  VS EVKRP+KSRSK EKE+EE
Sbjct: 433 NRLRS-KATTTTKLIN--------------KFPLTLDSTVSFEVKRPKKSRSKSEKEDEE 492

Query: 515 EVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVNIPHLRGKG----KTEKT 574
           EVLVI+ I+      +KFDV INDED+    AD TEFAGSFVN+PH    G    K + T
Sbjct: 493 EVLVIEKIKHEPQFPLKFDVYINDEDEDPSAADQTEFAGSFVNVPHFHRHGDKKDKRQTT 552

Query: 575 CFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIE 594
              +GISE+L++LD D DDS++VTLVP+ G  ++ +G  KIE
Sbjct: 553 NLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of Cla97C03G057130 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 4.3e-160
Identity = 297/606 (49.01%), Postives = 395/606 (65.18%), Query Frame = 0

Query: 7   TSLSPTSPPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPD- 66
           T++S   P PL   A+  PV   R+ +  P              S+VSC+++N   + D 
Sbjct: 18  TNISAFPPSPLFQRASHVPVARNRSRRFAP--------------SKVSCNSANGDPNSDS 77

Query: 67  ----------KFDRRNLLLGLGGLYGAS--TLSPNPLASANPIVTPPLDTCGPTDLPAGA 126
                     K DRRN+LLG+GGLYGA+    +  PLA   PI  P +  CG   +P G 
Sbjct: 78  TSDVRETSSGKLDRRNVLLGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGV 137

Query: 127 KPTDCCPPPTVKIKDFR-PTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSF 186
            PT+CCPP T KI DF+ P+    +R RPAAHL    +  KYK+A+E+ K+LP+DDPRSF
Sbjct: 138 TPTNCCPPVTTKIIDFQLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSF 197

Query: 187 TQQANVHCAYCNGGYDQLGF-PVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALP 246
            QQANVHC YC G YDQ+G+  +ELQVH SWLF PFHRYYLYF+E+IL +LI DP FALP
Sbjct: 198 KQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALP 257

Query: 247 FWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEGDENAQIRS-NLG 306
           +W +D P GM MP IYA   S LYDE RN  H+PP ++DLD++  +    + ++++ NL 
Sbjct: 258 YWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLA 317

Query: 307 VMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNF 366
           +MY+Q+VS + TP LF G  YRAGD   PG G +E+ PH  VH+W G  ++P+ E+MGNF
Sbjct: 318 IMYKQIVSGATTPKLFLGYPYRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNF 377

Query: 367 YSAARDPIFYAHHSNVDRMWSIWKTLGGK-RKDIQNPDYLNASFLFYNEKAEAVRVYVRD 426
           Y+A RDPIF+ HH+NVDRMW+IWKT+GGK RKD  + D+L+A+F+FY+E  + V+V V D
Sbjct: 378 YTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSD 437

Query: 427 SLDTKTLGYVFQDVDIPWL--KSRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVSF 486
            +DT  L Y +QD+ IPWL   ++   + T KS K     A+  P     ++ +     F
Sbjct: 438 CVDTSKLRYQYQDIPIPWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGD-----F 497

Query: 487 PLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRA 546
           P  L+ ++ +EV RP+KSRSKKEKE+EEEVL+I GIE +    VKFDV INDED    R 
Sbjct: 498 PKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRP 557

Query: 547 DNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIV 594
            N+EFAGSFVN+PH   K    KT  R  I+ELLEDL A+DD+SVIVT+VP+ G D + +
Sbjct: 558 KNSEFAGSFVNVPHKHMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTI 603

BLAST of Cla97C03G057130 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.4e-233
Identity = 403/598 (67.39%), Postives = 474/598 (79.26%), Query Frame = 0

Query: 12  TSPPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPDKFDRRN 71
           TSPP LSSAATT     F  N K PP    RTK+  +   R+ CS SN G   +  DRR+
Sbjct: 3   TSPPALSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQ--NHIDRRD 62

Query: 72  LLLGLGGLYGASTLSPNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFRP 131
           +L+GLGGLYGASTLS   LA A P+ TP +  CG  DLP GA P +CCPPPT  + DFRP
Sbjct: 63  ILIGLGGLYGASTLSQGSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRP 122

Query: 132 TPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQLG 191
             P +LRVRPAAHLAD N+ EKYKRAVE+MK+LP+DDPRSF QQANVHCAYCNG YDQ+G
Sbjct: 123 QAPGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVG 182

Query: 192 FPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADKS 251
           FPVE+QVHNSWLFFPFHRYYL+FHEKILGELIGDP+FALPFWNYDAP GM+MPA+YAD+ 
Sbjct: 183 FPVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEK 242

Query: 252 SPLYDELRNQSHVPPALVDLDFNDADE--GDENAQIRSNLGVMYRQVVSASRTPLLFFGG 311
           S LYDELRN++H+PP LVDLDF   D   GDE AQIRSNL +MYRQ+VS SRTP LFFG 
Sbjct: 243 SSLYDELRNRNHLPPTLVDLDFGGVDPTIGDE-AQIRSNLSIMYRQMVSNSRTPSLFFGN 302

Query: 312 AYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRM 371
           AY AGD PSPGGG+VENIPHGPVH WCGDN +PN ENMGNFYSAARDPIFYAHHSN+DR+
Sbjct: 303 AYLAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRL 362

Query: 372 WSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLK 431
           WS+WKTLGG+R+D+QN D+LNASF+FY+EKA+AVRV VRD LD++ LGYV+QDV +PWL+
Sbjct: 363 WSVWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLE 422

Query: 432 SRPTPRKTLKSRKPRATTASISPFDVGPALA-----------EPVPVSFPLTLDKIVSIE 491
           SRPTPR+ L+++K  A      PF VGPALA            P  + FP+TL+  +S+E
Sbjct: 423 SRPTPRRALRAQKAVAHL----PFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISME 482

Query: 492 VKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVN 551
           VKRP++SRSKKEK+EEEE++VI GIEF++N A+KFDV INDEDD+EIR DNTEFAGSFVN
Sbjct: 483 VKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVN 542

Query: 552 IPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIELEE 597
           +PH  G  K  KT  RLGI+ELLEDLDADDDDSV VTLVP+ G   + +  +KIE ++
Sbjct: 543 VPHKHGHKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEFDK 593

BLAST of Cla97C03G057130 vs. ExPASy TrEMBL
Match: A0A5D3DS75 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00570 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.2e-221
Identity = 373/558 (66.85%), Postives = 453/558 (81.18%), Query Frame = 0

Query: 47  VNNVSRVSCSTSNN---GSSPDKFDRRNLLLGLGGLYGASTLSPNP-LASANPIVTPPLD 106
           ++  SR+SC  SN+    +S  K DRR++L+GL GLYG+STLS +P  A A PI TP + 
Sbjct: 22  ISITSRISCKASNHNDQNTSNPKIDRRDILIGLTGLYGSSTLSQSPHFALAAPIQTPNIS 81

Query: 107 TCGPTDLPAGAKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMK 166
            CGP DLP GA PT+CCPPP   I DF+P PP +LRVRPAAHLAD N+ EKYK+A+E+MK
Sbjct: 82  KCGPPDLPPGADPTNCCPPPATTILDFQPPPPTKLRVRPAAHLADQNYIEKYKKAIELMK 141

Query: 167 SLPEDDPRSFTQQANVHCAYCNGGYDQLGFPVELQVHNSWLFFPFHRYYLYFHEKILGEL 226
           +LPEDDPRSFTQQANVHCAYCNG YDQ+GFPVE+QVHNSWLFFPFHR+YLYFHEKILGEL
Sbjct: 142 ALPEDDPRSFTQQANVHCAYCNGAYDQIGFPVEVQVHNSWLFFPFHRFYLYFHEKILGEL 201

Query: 227 IGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEGD-- 286
           IGD +FALPFWNYDAP GM+MP +YA++ S LYDELRN++H+PP LVDLDF   D+ +  
Sbjct: 202 IGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYDELRNENHLPPRLVDLDFGGVDDPNIG 261

Query: 287 ENAQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNE 346
           ++AQIRSNL +MYRQ+VS SRTP LFFG AY  GD PSPGGG VENIPHGPVH WCGDN+
Sbjct: 262 DDAQIRSNLSIMYRQMVSGSRTPSLFFGSAYVEGDEPSPGGGSVENIPHGPVHVWCGDND 321

Query: 347 QPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKA 406
           QPN ENMGNFYSAARDPIF+AHHSNVDR+WS+WKTLGG+RKD+QNP++LNASF+FYNEKA
Sbjct: 322 QPNFENMGNFYSAARDPIFFAHHSNVDRLWSVWKTLGGRRKDLQNPEWLNASFIFYNEKA 381

Query: 407 EAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALA 466
           + VRV+VRD LD+K+LGY +Q VD+PWL++RPTPR   +++K        S    GP+ +
Sbjct: 382 QPVRVHVRDCLDSKSLGYTYQPVDLPWLQTRPTPR---RAQKTTVAFGIASALAAGPSSS 441

Query: 467 --EPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLIN 526
             +P  + FP+T+   VS+EVKRP+KSRSKKEKEEEEEVLVI GI F+ N  +KFDV IN
Sbjct: 442 RVKPANIKFPITVKSPVSVEVKRPKKSRSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFIN 501

Query: 527 DEDDREIRADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVP 586
           DEDD+EI+ DNTEFAGSFVN+PH +   K  KT  R+GI+E+LEDL+AD+DDS+IVT+VP
Sbjct: 502 DEDDKEIKPDNTEFAGSFVNVPHKQAHKKKVKTGLRVGITEVLEDLEADNDDSIIVTIVP 561

Query: 587 KFGYDKLIVGKVKIELEE 597
           K G     +G +KIEL+E
Sbjct: 562 KIGAGLFSIGGLKIELDE 576

BLAST of Cla97C03G057130 vs. ExPASy TrEMBL
Match: A0A1S3B632 (polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103486180 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.2e-221
Identity = 373/558 (66.85%), Postives = 453/558 (81.18%), Query Frame = 0

Query: 47  VNNVSRVSCSTSNN---GSSPDKFDRRNLLLGLGGLYGASTLSPNP-LASANPIVTPPLD 106
           ++  SR+SC  SN+    +S  K DRR++L+GL GLYG+STLS +P  A A PI TP + 
Sbjct: 22  ISITSRISCKASNHNDQNTSNPKIDRRDILIGLTGLYGSSTLSQSPHFALAAPIQTPNIS 81

Query: 107 TCGPTDLPAGAKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMK 166
            CGP DLP GA PT+CCPPP   I DF+P PP +LRVRPAAHLAD N+ EKYK+A+E+MK
Sbjct: 82  KCGPPDLPPGADPTNCCPPPATTILDFQPPPPTKLRVRPAAHLADQNYIEKYKKAIELMK 141

Query: 167 SLPEDDPRSFTQQANVHCAYCNGGYDQLGFPVELQVHNSWLFFPFHRYYLYFHEKILGEL 226
           +LPEDDPRSFTQQANVHCAYCNG YDQ+GFPVE+QVHNSWLFFPFHR+YLYFHEKILGEL
Sbjct: 142 ALPEDDPRSFTQQANVHCAYCNGAYDQIGFPVEVQVHNSWLFFPFHRFYLYFHEKILGEL 201

Query: 227 IGDPNFALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEGD-- 286
           IGD +FALPFWNYDAP GM+MP +YA++ S LYDELRN++H+PP LVDLDF   D+ +  
Sbjct: 202 IGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYDELRNENHLPPRLVDLDFGGVDDPNIG 261

Query: 287 ENAQIRSNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNE 346
           ++AQIRSNL +MYRQ+VS SRTP LFFG AY  GD PSPGGG VENIPHGPVH WCGDN+
Sbjct: 262 DDAQIRSNLSIMYRQMVSGSRTPSLFFGSAYVEGDEPSPGGGSVENIPHGPVHVWCGDND 321

Query: 347 QPNGENMGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKA 406
           QPN ENMGNFYSAARDPIF+AHHSNVDR+WS+WKTLGG+RKD+QNP++LNASF+FYNEKA
Sbjct: 322 QPNFENMGNFYSAARDPIFFAHHSNVDRLWSVWKTLGGRRKDLQNPEWLNASFIFYNEKA 381

Query: 407 EAVRVYVRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALA 466
           + VRV+VRD LD+K+LGY +Q VD+PWL++RPTPR   +++K        S    GP+ +
Sbjct: 382 QPVRVHVRDCLDSKSLGYTYQPVDLPWLQTRPTPR---RAQKTTVAFGIASALAAGPSSS 441

Query: 467 --EPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLIN 526
             +P  + FP+T+   VS+EVKRP+KSRSKKEKEEEEEVLVI GI F+ N  +KFDV IN
Sbjct: 442 RVKPANIKFPITVKSPVSVEVKRPKKSRSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFIN 501

Query: 527 DEDDREIRADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLVP 586
           DEDD+EI+ DNTEFAGSFVN+PH +   K  KT  R+GI+E+LEDL+AD+DDS+IVT+VP
Sbjct: 502 DEDDKEIKPDNTEFAGSFVNVPHKQAHKKKVKTGLRVGITEVLEDLEADNDDSIIVTIVP 561

Query: 587 KFGYDKLIVGKVKIELEE 597
           K G     +G +KIEL+E
Sbjct: 562 KIGAGLFSIGGLKIELDE 576

BLAST of Cla97C03G057130 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.6e-218
Identity = 376/584 (64.38%), Postives = 457/584 (78.25%), Query Frame = 0

Query: 14  PPPLSSAATTFPVPLFRTNKKTPPLIPPRTKFLVNNVSRVSCSTSNNGSSPDKFDRRNLL 73
           PPP  S AT      FR + +     PP +K+L NN +RV CS +N      K DRR++L
Sbjct: 5   PPPALSTAT------FRPSSR-----PPTSKYLPNNAARVLCSAANG-----KIDRRDVL 64

Query: 74  LGL--GGLYGASTLS-PNPLASANPIVTPPLDTCGPTDLPAGAKPTDCCPPPTVKIKDFR 133
           +G+  GG+YGAS  +  +  A A P+ TP +  CGP DLP G+ PT+CCPPP+  I DFR
Sbjct: 65  IGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFR 124

Query: 134 PTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPEDDPRSFTQQANVHCAYCNGGYDQL 193
           P  P +LRVRPAAHLAD ++ +KYKRAVE+MK LP DDPRSF QQANVHCAYCNGGYDQ+
Sbjct: 125 PPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQV 184

Query: 194 GFPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPNFALPFWNYDAPVGMRMPAIYADK 253
           GFPVE+QVHNSWLFFPFHRYYLYF+EKILGELIGDP FALPFWNYDAP GMRMPA+YAD 
Sbjct: 185 GFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADH 244

Query: 254 SSPLYDELRNQSHVPPALVDLDFNDADEG-DENAQIRSNLGVMYRQVVSASRTPLLFFGG 313
           +S LYD+LRNQ+H+PP L+DLDF+  D    +NAQIRSNL +MYRQ+VS SRTP LFFG 
Sbjct: 245 NSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGS 304

Query: 314 AYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGENMGNFYSAARDPIFYAHHSNVDRM 373
           AY AGD PSPGGG VENIPH  VH WCGDN +PN ENMGNFYSAARDPIFY+HHSNVDRM
Sbjct: 305 AYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRM 364

Query: 374 WSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVYVRDSLDTKTLGYVFQDVDIPWLK 433
           WS+WKTLGG+R+D+Q P++L+ASF+FYNEKA+AVRV VRD LDTK LGY++QDV++PWL+
Sbjct: 365 WSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLE 424

Query: 434 SRPTPRKTLKSRKPRATTASISPFDVGPALAEPVPVSFPLTLDKIVSIEVKRPRKSRSKK 493
           +RPTPR+ L+++K      S+   +V     +P  +  P+TL   +S EVKR +KSR +K
Sbjct: 425 TRPTPRRALRAQK------SVDS-NVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRK 484

Query: 494 EKEEEEEVLVIDGIEFNSNTAVKFDVLINDEDDREIRADNTEFAGSFVNIPHLRGKGKTE 553
           EK+EEEE+LVI GIEF+ NT++K D+ INDEDDREIR DNTEFAGSFVN+PH  G  K  
Sbjct: 485 EKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKV 544

Query: 554 KTCFRLGISELLEDLDADDDDSVIVTLVPKFGYDKLIVGKVKIE 594
           KT  RLGISE+LEDLDADDDDS+IVT++P+ G   + + ++KIE
Sbjct: 545 KTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIE 565

BLAST of Cla97C03G057130 vs. ExPASy TrEMBL
Match: A0A0A0LXD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.8e-215
Identity = 364/559 (65.12%), Postives = 442/559 (79.07%), Query Frame = 0

Query: 52  RVSCSTSNNGSS---PDKFDRRNLLLGLGGLYGASTLSPNP-LASANPIVTPPLDTCGPT 111
           R+SC  +N+        K DRR++L+GL GLYG+STLS +   A A PI TP +  CGP 
Sbjct: 27  RISCKATNHNDQNFPNPKIDRRDILIGLTGLYGSSTLSQSTHFALAAPIETPNISKCGPP 86

Query: 112 DLPAGAKPTDCCPPPTVKIKDFRPTPPRQLRVRPAAHLADNNFTEKYKRAVEIMKSLPED 171
           DLP GA  T+CCPPP   I DF+P PP +LRVRPAAHLAD N+ EKYK+AVE+MK+LPED
Sbjct: 87  DLPPGADATNCCPPPATTILDFQPPPPSKLRVRPAAHLADRNYIEKYKKAVELMKALPED 146

Query: 172 DPRSFTQQANVHCAYCNGGYDQLGFPVELQVHNSWLFFPFHRYYLYFHEKILGELIGDPN 231
           DPRSFTQQANVHCAYCNG YDQ+GFPVE+QVHNSWLFFPFHR+YLYFHEKILGELIGDP+
Sbjct: 147 DPRSFTQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPS 206

Query: 232 FALPFWNYDAPVGMRMPAIYADKSSPLYDELRNQSHVPPALVDLDFNDADEG-DENAQIR 291
           FALPFWNYDAP GM+MP +YA++ S LYDELRN++H+PP LVDLDF   D    ++AQI+
Sbjct: 207 FALPFWNYDAPDGMKMPGMYAEEKSSLYDELRNENHLPPRLVDLDFGGVDPNIGDDAQIK 266

Query: 292 SNLGVMYRQVVSASRTPLLFFGGAYRAGDGPSPGGGVVENIPHGPVHRWCGDNEQPNGEN 351
           SNL +MYRQ+VS SRTP LFFG AY AGD PSPGGG VENIPHGPVH WCGD +QPN EN
Sbjct: 267 SNLSIMYRQMVSGSRTPSLFFGSAYVAGDEPSPGGGSVENIPHGPVHVWCGDTDQPNFEN 326

Query: 352 MGNFYSAARDPIFYAHHSNVDRMWSIWKTLGGKRKDIQNPDYLNASFLFYNEKAEAVRVY 411
           MGNFYSAARDPIF+AHHSN+DR+WS+WKTLGG+R+++QNP++LNASF+FY+EKA+ +RV 
Sbjct: 327 MGNFYSAARDPIFFAHHSNIDRLWSVWKTLGGRRQNLQNPEWLNASFIFYDEKAQPIRVR 386

Query: 412 VRDSLDTKTLGYVFQDVDIPWLKSRPTPRKTLKSRKPRATTASISPFDVGPALA------ 471
           VRD LD+K+LGY++Q VD+PWL++RPTPR        RA   S   F + PALA      
Sbjct: 387 VRDCLDSKSLGYIYQPVDLPWLQTRPTPR--------RAQKTSTVAFGIAPALAAGGPSS 446

Query: 472 ---EPVPVSFPLTLDKIVSIEVKRPRKSRSKKEKEEEEEVLVIDGIEFNSNTAVKFDVLI 531
              +P  + FP+T+   VS+EVKRP+KSRSKKEKEEEEEVLVI GI F+ N  +KFDV I
Sbjct: 447 SRVKPANIKFPITVKSPVSVEVKRPKKSRSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFI 506

Query: 532 NDEDDREIRADNTEFAGSFVNIPHLRGKGKTEKTCFRLGISELLEDLDADDDDSVIVTLV 591
           NDEDD+EI+ DNTEFAGSFVN+PH  G  K  KT  R+GI+E LEDL+AD+DDS+IVT++
Sbjct: 507 NDEDDKEIKPDNTEFAGSFVNVPHKHGHKKKVKTGLRVGITEELEDLEADNDDSIIVTIL 566

Query: 592 PKFGYDKLIVGKVKIELEE 597
           P+ G     +  +KIE ++
Sbjct: 567 PRIGAGLFSIDGLKIEFDK 577

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APO14262.12.8e-23367.39polyphenol oxidase 3 [Luffa aegyptiaca][more]
XP_038903169.13.1e-22467.90polyphenol oxidase, chloroplastic-like [Benincasa hispida][more]
XP_008442274.12.5e-22166.85PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056968.1 p... [more]
XP_023001713.13.3e-21864.38polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
KAG6584007.11.2e-21564.04hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P433096.6e-19356.88Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q062153.0e-17757.24Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
O811031.2e-16250.49Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PP... [more]
Q6UIL35.1e-16153.26(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
P433114.3e-16049.01Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1L5JHU31.4e-23367.39Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A5D3DS751.2e-22166.85Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G0... [more]
A0A1S3B6321.2e-22166.85polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103486180 P... [more]
A0A6J1KRC01.6e-21864.38polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A0A0LXD02.8e-21565.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 587..596
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 94..595
e-value: 7.2E-224
score: 742.1
NoneNo IPR availablePIRSRPIRSR000290-2PIRSR000290-2coord: 94..595
e-value: 7.2E-224
score: 742.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 50..595
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 50..595
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 199..216
score: 57.6
coord: 230..235
score: 55.1
coord: 355..373
score: 58.86
coord: 328..339
score: 39.29
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 169..375
e-value: 1.2E-31
score: 110.7
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 356..367
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 199..216
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 1..596
e-value: 2.4E-247
score: 820.4
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 382..433
e-value: 5.3E-21
score: 74.1
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 467..594
e-value: 4.4E-50
score: 169.1
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 94..429
e-value: 1.9E-113
score: 381.3
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 108..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C03G057130.1Cla97C03G057130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity