ClCG11G000200 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG11G000200
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionPectinesterase
LocationCG_Chr11: 260639 .. 262646 (+)
RNA-Seq ExpressionClCG11G000200
SyntenyClCG11G000200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGTTCCAAGATTTCGACCAAATCTCTGAACGCCGACGCGCCGAGAGGGCACGTAAGTTTCGAAAACGAGTCACTATCGCTGTGGTCTCGGCCTCTGTGATCCTCCTCCTCGTTGCTGCCGCTGTGTTCGTCCAGGTCTCCTCCTCCTCCAAGTCCACCACCAAAAATGGCGATAGCAAGAGTAGTGGCAACTCTAAACAACCCGTGCCACCCGACACGAAACAAGTGTCGCGCATCGAGAAAATGATTACCATGATATGCAACTCGACAGACTACAAAGGCAAATGCGAGAGCACTCTAAAAGATGGCGTCCACACGGATCCTGATTCCTCGGACCCGAAAGACCTCATCAAGCTGGCAATCTCGGCTGCGGCGGATGAGGTCAAATCTGCAGTGAAAAAGGCGTCGGGGTTCAACTTCGCGACGCCGGAGGAAAAGGGGGCGTTCGAGGACTGCAAGGTGCTGTTGGAAGACGCTGTGGAGGAATTGGAGATGTCTATGAGCGAAGTGAGCAAGAAGAATATGGGAAAGCTGACGGCGAAAACGACGCCGGATCTGAATAACTGGCTTAGTGCGGTGATGTCGTATCATGAAACTTGTGTTGATGGGTTCCCAGAGGGGAAGATGAGGACAGAAATGGAGAAGGCAGTGAAGGCTGGAAAGGAACTCACCAGTAATTCCTTGGCGATGATATCGCAGGTGGCTTCCTTCTTCTCCACGTTCGAGATGCCTGAGGGAGCTGCAACACGACGTCGTCTCATGGCCAATGATGGGGTGCCAACTTGGATGGATAGCAATGAGCGAAGGATGTTGAAGGGCGCTTCTGCTGGGGTTAAGCCCAAGCCTAACGTTGTGGTCGCCAAAGATGGGTCTGGAGATTTCAAAACTATTAATGAAGCCTTGGCCAAGATGCCTGCCAAATATGATGGACGGTATACCAAATATCATTCATATTATATATATACTCTACTTGGTTTTATTTTTTAAATCATCATATTCATATTCTTGTTCATAACATGTGTTGCATTTGACATTTTTTTTTAATAATAATAATAAATAAAAAAAGCAGGTATGTGATCTATGTGAAGGAAGGAATCTACGAAGAGACGGTGGTGATTACGAAAAAGATGGCGAATGTTACAATGTATGGAGATGGTTCTCAAAAGAGCATGATAAGTGGGAGCAAGAACTTTGTGGATGGAGTCAGAACCTTCCAAACCGCAACATTTGGTAATTAAGCATTAATTGATTAGCATTAAAAATGAAAGAATTGGGTAATTAGGAGAAAGAATTTTGTACATTTTAAATTTATGCAGTGGCACTTGGAGAGGGCTTCTTGGGGCAAGGCATGGGATTCAGAAACACAGCGGGTCCTGAAAAGCACCAAGCCGTAGCAGCGAGAGTCCAAGCAGACCGAGCCATATTTGTGAACTGCCGGTTCGAGGGGTACCAGGACACGTTATACGCCCAAGCCCATCGCCAGTTCTATAGAAGCTGTCTGATAACAGGCACCATCGACTTCATCTTCGGTGACGCCGCTGCCATTTTCCAAAACTGCAACATGATGCTCAGAAAGCCCTTAGACAACCAACAGAACATAGTCACAGCACAGGGCAGGACTGACAAGCATGAAACCACAGGCATAGTCCTCCAAAACTGCAAAATTTTGCCCGACAAGACGTTGGAGCCTGTGAAGTCCCAATTCAAAAGCTACTTGGGGAGGCCTTGGAAAGAGTTCTCTAGAACCATTGTAATGGAGTCCACCATTGAAGATATCATTCATCCCGATGGCTGGATGCCTTGGGAAGGAGATTTTGCACTCAAAACCTTGTACTATGCTGAATTTAACAACCAAGGCCCTGGGGCTAAGACCGATGCCAGGGTCAAATGGCCCGGGTACAAGGTGATTGATAAGGACGAGGCTGCAAAGTTTACCATAGGAACTTTTCTAGAGCTGGATTGGATCGAGAGCTCCTCCGCTCCTGTTCATGTCGGCCTATTTTGA

mRNA sequence

ATGGTGTTCCAAGATTTCGACCAAATCTCTGAACGCCGACGCGCCGAGAGGGCACGTAAGTTTCGAAAACGAGTCACTATCGCTGTGGTCTCGGCCTCTGTGATCCTCCTCCTCGTTGCTGCCGCTGTGTTCGTCCAGGTCTCCTCCTCCTCCAAGTCCACCACCAAAAATGGCGATAGCAAGAGTAGTGGCAACTCTAAACAACCCGTGCCACCCGACACGAAACAAGTGTCGCGCATCGAGAAAATGATTACCATGATATGCAACTCGACAGACTACAAAGGCAAATGCGAGAGCACTCTAAAAGATGGCGTCCACACGGATCCTGATTCCTCGGACCCGAAAGACCTCATCAAGCTGGCAATCTCGGCTGCGGCGGATGAGGTCAAATCTGCAGTGAAAAAGGCGTCGGGGTTCAACTTCGCGACGCCGGAGGAAAAGGGGGCGTTCGAGGACTGCAAGGTGCTGTTGGAAGACGCTGTGGAGGAATTGGAGATGTCTATGAGCGAAGTGAGCAAGAAGAATATGGGAAAGCTGACGGCGAAAACGACGCCGGATCTGAATAACTGGCTTAGTGCGGTGATGTCGTATCATGAAACTTGTGTTGATGGGTTCCCAGAGGGGAAGATGAGGACAGAAATGGAGAAGGCAGTGAAGGCTGGAAAGGAACTCACCAGTAATTCCTTGGCGATGATATCGCAGGTGGCTTCCTTCTTCTCCACGTTCGAGATGCCTGAGGGAGCTGCAACACGACGTCGTCTCATGGCCAATGATGGGGTGCCAACTTGGATGGATAGCAATGAGCGAAGGATGTTGAAGGGCGCTTCTGCTGGGGTTAAGCCCAAGCCTAACGTTGTGGTCGCCAAAGATGGGTCTGGAGATTTCAAAACTATTAATGAAGCCTTGGCCAAGATGCCTGCCAAATATGATGGACGGTATGTGATCTATGTGAAGGAAGGAATCTACGAAGAGACGGTGGTGATTACGAAAAAGATGGCGAATGTTACAATGTATGGAGATGGTTCTCAAAAGAGCATGATAAGTGGGAGCAAGAACTTTGTGGATGGAGTCAGAACCTTCCAAACCGCAACATTTGTGGCACTTGGAGAGGGCTTCTTGGGGCAAGGCATGGGATTCAGAAACACAGCGGGTCCTGAAAAGCACCAAGCCGTAGCAGCGAGAGTCCAAGCAGACCGAGCCATATTTGTGAACTGCCGGTTCGAGGGGTACCAGGACACGTTATACGCCCAAGCCCATCGCCAGTTCTATAGAAGCTGTCTGATAACAGGCACCATCGACTTCATCTTCGGTGACGCCGCTGCCATTTTCCAAAACTGCAACATGATGCTCAGAAAGCCCTTAGACAACCAACAGAACATAGTCACAGCACAGGGCAGGACTGACAAGCATGAAACCACAGGCATAGTCCTCCAAAACTGCAAAATTTTGCCCGACAAGACGTTGGAGCCTGTGAAGTCCCAATTCAAAAGCTACTTGGGGAGGCCTTGGAAAGAGTTCTCTAGAACCATTGTAATGGAGTCCACCATTGAAGATATCATTCATCCCGATGGCTGGATGCCTTGGGAAGGAGATTTTGCACTCAAAACCTTGTACTATGCTGAATTTAACAACCAAGGCCCTGGGGCTAAGACCGATGCCAGGGTCAAATGGCCCGGGTACAAGGTGATTGATAAGGACGAGGCTGCAAAGTTTACCATAGGAACTTTTCTAGAGCTGGATTGGATCGAGAGCTCCTCCGCTCCTGTTCATGTCGGCCTATTTTGA

Coding sequence (CDS)

ATGGTGTTCCAAGATTTCGACCAAATCTCTGAACGCCGACGCGCCGAGAGGGCACGTAAGTTTCGAAAACGAGTCACTATCGCTGTGGTCTCGGCCTCTGTGATCCTCCTCCTCGTTGCTGCCGCTGTGTTCGTCCAGGTCTCCTCCTCCTCCAAGTCCACCACCAAAAATGGCGATAGCAAGAGTAGTGGCAACTCTAAACAACCCGTGCCACCCGACACGAAACAAGTGTCGCGCATCGAGAAAATGATTACCATGATATGCAACTCGACAGACTACAAAGGCAAATGCGAGAGCACTCTAAAAGATGGCGTCCACACGGATCCTGATTCCTCGGACCCGAAAGACCTCATCAAGCTGGCAATCTCGGCTGCGGCGGATGAGGTCAAATCTGCAGTGAAAAAGGCGTCGGGGTTCAACTTCGCGACGCCGGAGGAAAAGGGGGCGTTCGAGGACTGCAAGGTGCTGTTGGAAGACGCTGTGGAGGAATTGGAGATGTCTATGAGCGAAGTGAGCAAGAAGAATATGGGAAAGCTGACGGCGAAAACGACGCCGGATCTGAATAACTGGCTTAGTGCGGTGATGTCGTATCATGAAACTTGTGTTGATGGGTTCCCAGAGGGGAAGATGAGGACAGAAATGGAGAAGGCAGTGAAGGCTGGAAAGGAACTCACCAGTAATTCCTTGGCGATGATATCGCAGGTGGCTTCCTTCTTCTCCACGTTCGAGATGCCTGAGGGAGCTGCAACACGACGTCGTCTCATGGCCAATGATGGGGTGCCAACTTGGATGGATAGCAATGAGCGAAGGATGTTGAAGGGCGCTTCTGCTGGGGTTAAGCCCAAGCCTAACGTTGTGGTCGCCAAAGATGGGTCTGGAGATTTCAAAACTATTAATGAAGCCTTGGCCAAGATGCCTGCCAAATATGATGGACGGTATGTGATCTATGTGAAGGAAGGAATCTACGAAGAGACGGTGGTGATTACGAAAAAGATGGCGAATGTTACAATGTATGGAGATGGTTCTCAAAAGAGCATGATAAGTGGGAGCAAGAACTTTGTGGATGGAGTCAGAACCTTCCAAACCGCAACATTTGTGGCACTTGGAGAGGGCTTCTTGGGGCAAGGCATGGGATTCAGAAACACAGCGGGTCCTGAAAAGCACCAAGCCGTAGCAGCGAGAGTCCAAGCAGACCGAGCCATATTTGTGAACTGCCGGTTCGAGGGGTACCAGGACACGTTATACGCCCAAGCCCATCGCCAGTTCTATAGAAGCTGTCTGATAACAGGCACCATCGACTTCATCTTCGGTGACGCCGCTGCCATTTTCCAAAACTGCAACATGATGCTCAGAAAGCCCTTAGACAACCAACAGAACATAGTCACAGCACAGGGCAGGACTGACAAGCATGAAACCACAGGCATAGTCCTCCAAAACTGCAAAATTTTGCCCGACAAGACGTTGGAGCCTGTGAAGTCCCAATTCAAAAGCTACTTGGGGAGGCCTTGGAAAGAGTTCTCTAGAACCATTGTAATGGAGTCCACCATTGAAGATATCATTCATCCCGATGGCTGGATGCCTTGGGAAGGAGATTTTGCACTCAAAACCTTGTACTATGCTGAATTTAACAACCAAGGCCCTGGGGCTAAGACCGATGCCAGGGTCAAATGGCCCGGGTACAAGGTGATTGATAAGGACGAGGCTGCAAAGTTTACCATAGGAACTTTTCTAGAGCTGGATTGGATCGAGAGCTCCTCCGCTCCTGTTCATGTCGGCCTATTTTGA

Protein sequence

MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF
Homology
BLAST of ClCG11G000200 vs. NCBI nr
Match: XP_008460558.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo] >KAA0045465.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa] >TYK00244.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 581/595 (97.65%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDS 60
           MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILL+VAAAVFVQVSSSSKSTTKNGDS
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGDS 60

Query: 61  KSS-GNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIK 120
            SS GNSK+P+PP+TKQVSR+EKMITMICNSTDYKGKCESTLKDG+HTDP+SSDPKDLIK
Sbjct: 61  GSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLIK 120

Query: 121 LAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180
           LAISAAA EVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180

Query: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVASFF 240
           TAKTTPDLNNWLSAVMSYHETCVDGFPEGKM++EMEK VKAGKELTSNSLAMISQVASFF
Sbjct: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 STFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTIN 300
           STFEMPEGAA+RRRLMA  GVPTWM+SNERRMLKGA+AG KPKPNVVVAKDGSG+FKTIN
Sbjct: 241 STFEMPEGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 EALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRT 360
           EALA MPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISGSKNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420
           FQTATFVALGEGFLGQ +GFRN AGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCNMM+RKPLDNQQNIVTAQGRTDKHETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480

Query: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYY 540
           CKILPDKTLE VKSQFKSYLGRPWKEFSRTIVMES IED+IHPDGWM W+GDFALKTLYY
Sbjct: 481 CKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKTLYY 540

Query: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF 595
           AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES+SAPVHVGLF
Sbjct: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of ClCG11G000200 vs. NCBI nr
Match: XP_004142361.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus] >KGN52371.1 hypothetical protein Csa_008584 [Cucumis sativus])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 554/595 (93.11%), Postives = 578/595 (97.14%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDS 60
           MVFQDFDQISERRRAERARKFRKRV IAVVSASVILL+VAAAVFVQV SSSKSTTKN DS
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQV-SSSKSTTKNDDS 60

Query: 61  -KSSGNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIK 120
             SSGNSKQP+PP+TKQVSR+EKMITMICNSTDYKGKCESTLKDGVHTDP+SSDPKDLIK
Sbjct: 61  GSSSGNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKDLIK 120

Query: 121 LAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180
           LAISAAA EVKSAVKKASGFNFATPEEKGAFEDCKVLLEDA+EELEMSMSEV+KKNMGKL
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEVNKKNMGKL 180

Query: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVASFF 240
           TAKTTP+LNNWLSAVMSYHETCVDGFPEGKM++++EK VKAGKELTSNSLAMISQVASFF
Sbjct: 181 TAKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 STFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTIN 300
           STFEMPEGAA+RRRLM  +GVPTWMD N+RRMLKGA+AG KPKPNVVVAKDGSG+FKTIN
Sbjct: 241 STFEMPEGAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 EALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRT 360
           EALA MPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISGSKNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420
           FQTATFVALGEGFLGQ +GFRN AGPEKHQAVAARVQADRAIFVNCRFEGYQDTLY QAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYTQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCNMM+RKPLDNQQN+VTAQGRTDKHETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTGIVLQN 480

Query: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYY 540
           CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIED+IHPDGWM WEGDFALKTLYY
Sbjct: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFALKTLYY 540

Query: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF 595
           AEFNN+GPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES+SAPVHVGLF
Sbjct: 541 AEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 594

BLAST of ClCG11G000200 vs. NCBI nr
Match: XP_038893171.1 (putative pectinesterase/pectinesterase inhibitor 45 [Benincasa hispida])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 553/597 (92.63%), Postives = 574/597 (96.15%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDS 60
           MVFQDFDQISERRRAERARKFRKRVTIAVVS SVILL++AAAVFVQVSSSSKSTTKNG+S
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSVSVILLVIAAAVFVQVSSSSKSTTKNGNS 60

Query: 61  KSSG---NSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDL 120
           KSS    NSK+ VP D KQVSR+EKMITMICNSTDYKGKCESTLKDG+HTD +SSDPKDL
Sbjct: 61  KSSSSNENSKETVPDDVKQVSRVEKMITMICNSTDYKGKCESTLKDGIHTDSNSSDPKDL 120

Query: 121 IKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMG 180
           IKL+ISAAADEVKSAVKKA+GFNFAT EEKGAFEDCKVLLEDAVEELEMSMS+VS KNMG
Sbjct: 121 IKLSISAAADEVKSAVKKATGFNFATAEEKGAFEDCKVLLEDAVEELEMSMSQVSIKNMG 180

Query: 181 KLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVAS 240
           KLTAKTTPDLNNWLSAVMSYHETCVDGFPEGK++TEMEK VK GKELTSNSLAMISQVAS
Sbjct: 181 KLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKIKTEMEKVVKNGKELTSNSLAMISQVAS 240

Query: 241 FFSTFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKT 300
           FFSTFEMPEGAATRRRLMA++G PTWM+SNERRMLKGA+AG KPKPNVVVAKDGSGDFKT
Sbjct: 241 FFSTFEMPEGAATRRRLMASNGFPTWMNSNERRMLKGATAGDKPKPNVVVAKDGSGDFKT 300

Query: 301 INEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGV 360
           INEALA MPAKYDGRYVIYVKEGIYEETVVITKKM NVTMYGDGSQKSMISG+KNFVDGV
Sbjct: 301 INEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMISGNKNFVDGV 360

Query: 361 RTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQ 420
           RTFQTATFVALGEGF  Q +GFRNTAGPEKHQAVAARVQADRAIF+NCRFEGYQDTLY Q
Sbjct: 361 RTFQTATFVALGEGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLYVQ 420

Query: 421 AHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVL 480
           AHRQFYRSCLITGTIDFIFGDAAA FQNCNMM+RKPLDNQQNIVTAQGRTDKHETTGIVL
Sbjct: 421 AHRQFYRSCLITGTIDFIFGDAAAFFQNCNMMVRKPLDNQQNIVTAQGRTDKHETTGIVL 480

Query: 481 QNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTL 540
           QNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTL
Sbjct: 481 QNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTL 540

Query: 541 YYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF 595
           YYAEFNNQGPGAKTD RVKWPGYKVIDKDEAAKFTIGTFLELDWIES+SAPVHVGLF
Sbjct: 541 YYAEFNNQGPGAKTDGRVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 597

BLAST of ClCG11G000200 vs. NCBI nr
Match: XP_022971606.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucurbita maxima])

HSP 1 Score: 961.1 bits (2483), Expect = 4.5e-276
Identity = 486/603 (80.60%), Postives = 534/603 (88.56%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNG-- 60
           MVFQDFDQISERRRAERARKFR RV IAVVS  +IL+ V AAVFVQVS  +KSTT++G  
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVFVVAAVFVQVSPPTKSTTRDGAG 60

Query: 61  -----DSKSSGNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSD 120
                 S+S G+SK     D KQVSR +KMITMICNSTDYKGKCESTLK GV T PDSSD
Sbjct: 61  AGSSSSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKQGVETHPDSSD 120

Query: 121 PKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSK 180
           PKDLIKLAI+A + EV SA+KKAS FNF TPEEKGAFEDCK+LLE+AVEEL+ S+SE+S+
Sbjct: 121 PKDLIKLAIAAVSTEVNSALKKASAFNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSR 180

Query: 181 KNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMIS 240
           K++GKLT KTTPDLNNWLSAVMSYH TCVDGFPEGK +T ME  +KAGKELTSNSLAMIS
Sbjct: 181 KDLGKLTDKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTVMEATLKAGKELTSNSLAMIS 240

Query: 241 QVASFFSTFEMPE--GAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDG 300
           QVA+FFSTFE  +  GAATRRRLMA DG+P+WM SNERRMLKG +A  KP PNVVVAKDG
Sbjct: 241 QVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSNERRMLKGDAADDKPTPNVVVAKDG 300

Query: 301 SGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSK 360
           SG+FKTINEALA MPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISG+K
Sbjct: 301 SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNK 360

Query: 361 NFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQ 420
           N  DGVR FQTA+FVALG+G L +G+GFRNTAGPEK QAVAARVQ DRAIFVNCRFEG+Q
Sbjct: 361 NSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQ 420

Query: 421 DTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHE 480
           DTLYAQAHRQFYRSCLITGT+D IFGDAAAIFQNCN ML+KPLD Q N+VTAQGR+D+HE
Sbjct: 421 DTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHE 480

Query: 481 TTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGD 540
           TT  VLQNCKILPDKTLE VK+QFKSYLGRP  E+SRTIVMES IEDIIHPDGWMPW+GD
Sbjct: 481 TTATVLQNCKILPDKTLEAVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGD 540

Query: 541 FALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHV 595
           FALKTLYYAEF+N+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+SSS  VHV
Sbjct: 541 FALKTLYYAEFSNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHV 600

BLAST of ClCG11G000200 vs. NCBI nr
Match: KAG6601658.1 (putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032419.1 putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 489/604 (80.96%), Postives = 536/604 (88.74%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQV----SSSSKSTTK 60
           MVFQDFDQISERRR ERARKFR+RV I VV A+VI+LLVAAAVF+QV    SSSSK++ K
Sbjct: 1   MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLVAAAVFIQVSPAPSSSSKTSNK 60

Query: 61  NGDSKS--SGNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDP 120
           +G SK     N+      D+K  S  EKMI MICNSTD+K KCES LKDG+  +P SSDP
Sbjct: 61  DGKSKKHPKSNADTKTDTDSKDASSAEKMIVMICNSTDFKEKCESILKDGLKANPKSSDP 120

Query: 121 KDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKK 180
           K+LIKLAI+AAA EVKSA+KKAS   FATPEEKGAFEDCKVL+EDAVEELEMSM+ VSKK
Sbjct: 121 KELIKLAITAAAAEVKSALKKASNLKFATPEEKGAFEDCKVLMEDAVEELEMSMTAVSKK 180

Query: 181 NMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEG-KMRTEMEKAVKAGKELTSNSLAMIS 240
           N GKLT KTTPDLNNWLSAVMSYHETCVDGFP+G KM+ EMEK  K+GKELTSNSLAMIS
Sbjct: 181 NQGKLTEKTTPDLNNWLSAVMSYHETCVDGFPDGSKMKQEMEKIGKSGKELTSNSLAMIS 240

Query: 241 QVASFFSTFEMP---EGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKD 300
           QVA+FFS FE     EGAATRRRLM  D +P+WMDSNERRMLKGA+AG KP PNVVVA+D
Sbjct: 241 QVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVVVAQD 300

Query: 301 GSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGS 360
           GSG+FKTINEALA MPAKYDGRYVIY+KEGIYEE VVITKKM NVTMYGDGSQKSMISG+
Sbjct: 301 GSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISGN 360

Query: 361 KNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGY 420
           KNF DGVRTFQTA+FVALG+GF  Q +GFRNTAGPEKHQAVAARVQADRAIF+NCRF+GY
Sbjct: 361 KNFKDGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFDGY 420

Query: 421 QDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKH 480
           QDTLYAQAHR FYRSCLITGTIDFIFGDAAAIFQNCNMM++KPLD QQNIVTAQGR+D+H
Sbjct: 421 QDTLYAQAHRHFYRSCLITGTIDFIFGDAAAIFQNCNMMVKKPLDKQQNIVTAQGRSDRH 480

Query: 481 ETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEG 540
           ETT IVLQ CKILPDKTLE VKSQFKSYLGRPWKE+SRTIVMESTIEDIIHP+GW+ WEG
Sbjct: 481 ETTAIVLQRCKILPDKTLEAVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPEGWLAWEG 540

Query: 541 DFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVH 595
           DFALKTLYYAEFNN GPGAKT+ RVKWPG+KVIDK+EAAKFTIG+FLE+DWI+S+SAPVH
Sbjct: 541 DFALKTLYYAEFNNDGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTSAPVH 600

BLAST of ClCG11G000200 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 5.9e-178
Identity = 317/609 (52.05%), Postives = 415/609 (68.14%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSK-------S 60
           M FQDFD+I ER  AER RKFRKR+ + VVS  V+   +    F  V+  +K       +
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  TTKNGDSKSSGNSKQPVPP----DTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDP 120
             K+ DS +   S  P PP     T +  +++K+I  +CNST YK  C++TLK+    D 
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120

Query: 121 DSSDPKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMS 180
             +DP+ L+K AI A  D++    K+         ++K A   CK+L+++A EEL  SM 
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180

Query: 181 EVSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSL 240
            ++   +    AK  PDL++WLSAVMSY ETCVDGF EGK++TE+ K   + + LTSNSL
Sbjct: 181 RINDSEVNNF-AKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSL 240

Query: 241 AMISQVASFFSTFEMPEG---AATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVV 300
           AMI  +  + S+    +       R      D + +W+ + ERRMLK        KPN  
Sbjct: 241 AMIKSLDGYLSSVPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKAL-KPNAT 300

Query: 301 VAKDGSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSM 360
           VAKDGSG+F TIN AL  MPAKY GRY IY+K GIY+E+V+I KK  NVTM GDGSQK++
Sbjct: 301 VAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTI 360

Query: 361 ISGSKNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCR 420
           ++G+K+    +RTF TATFVA GEGF+ Q MGFRNTAGPE HQAVA RVQ+DR++F+NCR
Sbjct: 361 VTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCR 420

Query: 421 FEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGR 480
           FEGYQDTLYA  HRQ+YRSC+I GT+DFIFGDAAAIFQNC++ +RK L  Q+N VTAQGR
Sbjct: 421 FEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGR 480

Query: 481 TDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWM 540
            DK +TTG V+ NC + P++ L+PVK+QFKSYLGRPWK  SRT+VMESTIED+I P GW+
Sbjct: 481 VDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWL 540

Query: 541 PW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESS 595
            W E DFA+ TL YAE+ N GP   T ARVKWPG++V++K+EA KFT+G FL+ +WI++ 
Sbjct: 541 RWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGEWIQAI 600

BLAST of ClCG11G000200 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 599.4 bits (1544), Expect = 4.6e-170
Identity = 310/620 (50.00%), Postives = 412/620 (66.45%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVS-------SSSKS 60
           M FQDFD+I ER  A R RKFRKR+ +  VS  V++  +    F  V+          + 
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  TTKNGDSKSSGNS-------KQPVPPDTKQVSRI-----------EKMITMICNSTDYKG 120
             KN +   SGN+       K P PP   Q + +           +K+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKV 180
            CE TLK+         +P   +K AI A  +++   ++K         ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEME 240
           L+EDA EE   S+++++   +     K  PDL +WLSAVMSY ETC+DGF EG +++E++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 KAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGA 300
            +V + + LTSNSLA+I       S    P      R L+  D +P+W+ +++RRML+  
Sbjct: 241 TSVNSSQVLTSNSLALIKTFTENLS----PVMKVVERHLL--DDIPSWVSNDDRRMLRAV 300

Query: 301 SAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANV 360
                 KPN  VAKDGSGDF TIN+AL  MP KY+GRY+IYVK+GIY+E V + KK AN+
Sbjct: 301 DVKAL-KPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANL 360

Query: 361 TMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARV 420
           TM GDGSQK++++G+K+    +RTF TATFVA GEGF+ Q MGFRNTAGPE HQAVA RV
Sbjct: 361 TMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRV 420

Query: 421 QADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLD 480
           Q+DR+IF+NCRFEGYQDTLYA  HRQ+YRSC+I GTIDFIFGDAAAIFQNCN+ +RK L 
Sbjct: 421 QSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLP 480

Query: 481 NQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMEST 540
            Q+N VTAQGR DK +TTG V+ NCKI  ++ L+PVK ++KSYLGRPWK +SRTI+MES 
Sbjct: 481 GQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESK 540

Query: 541 IEDIIHPDGWMPW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIG 595
           IE++I P GW+ W E DFA+ TLYYAE+NN+G    T +RVKWPG+KVI+K+EA  +T+G
Sbjct: 541 IENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVG 600

BLAST of ClCG11G000200 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.2e-130
Identity = 254/582 (43.64%), Postives = 360/582 (61.86%), Query Frame = 0

Query: 20  KFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTKQVSR 79
           K +K +   V++A ++L++VA  +          TT    S+++ +S++ VP    Q+  
Sbjct: 8   KKKKCIIAGVITALLVLMVVAVGI----------TT----SRNTSHSEKIVP---VQIKT 67

Query: 80  IEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGF 139
               +  +C  TDYK  C ++L   +   PDS+ P DLIKL  +     ++ ++KKAS  
Sbjct: 68  ATTAVEAVCAPTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVE 127

Query: 140 NFA----TPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVM 199
             A      + KGA E C+ L+ DA ++L+  +      ++ ++      DL  WLS  +
Sbjct: 128 LTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQI-EDFVEDLRVWLSGSI 187

Query: 200 SYHETCVDGFPE--GKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPE-----GA 259
           +Y +TC+D F E   K+  +M+K  K  +ELTSN LAMI+ +++    F +       G 
Sbjct: 188 AYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGK 247

Query: 260 ATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAK 319
             R+ L A DG+P+W+  N RR++     GV  K NVVVA DGSG +KTINEAL  +P  
Sbjct: 248 YARKLLSAEDGIPSWVGPNTRRLM-ATKGGV--KANVVVAHDGSGQYKTINEALNAVPKA 307

Query: 320 YDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDG-VRTFQTATFVA 379
               +VIY+K+G+Y E V +TKKM +VT  GDG  K+ I+GS N+  G V+T+ TAT   
Sbjct: 308 NQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAI 367

Query: 380 LGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCL 439
            G+ F  + +GF NTAGPE HQAVA RV AD A+F NC+ +GYQDTLY  +HRQF+R C 
Sbjct: 368 NGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCT 427

Query: 440 ITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKT 499
           ++GT+DFIFGD   + QNCN+++RKP+ +Q  ++TAQGR+DK E+TG+VLQNC I  +  
Sbjct: 428 VSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPA 487

Query: 500 LEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGP 559
             PVKS  K+YLGRPWKEFSRTI+M +TI+D+I P GW+PW GDFAL TLYYAE+ N GP
Sbjct: 488 YIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGP 547

Query: 560 GAKTDARVKWPGYKVIDKDEAAKFTIGTFLELD-WIESSSAP 589
           G+    RVKWPG K +   +A +FT   FL  + WI  +  P
Sbjct: 548 GSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVP 565

BLAST of ClCG11G000200 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.0e-129
Identity = 248/584 (42.47%), Postives = 361/584 (61.82%), Query Frame = 0

Query: 16  ERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTK 75
           E A++ ++ V I++ S  +I ++VA  + V V+ S  +    GD               +
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNA----GD---------------E 67

Query: 76  QVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKK 135
           +++   K I  +C  TDYK  CE TL+       D+SDP +L+K A +A   ++    KK
Sbjct: 68  EITTSVKAIKDVCAPTDYKETCEDTLRKDA---KDTSDPLELVKTAFNATMKQISDVAKK 127

Query: 136 ASGF--NFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSA 195
           +         P  K A + CK L++ A+ EL  S  E+ K    K+  +    L  WLSA
Sbjct: 128 SQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVD-EALVKLRIWLSA 187

Query: 196 VMSYHETCVDGF--PEGKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATR 255
            +S+ +TC+DGF   +G     ++KA+K   +LT N LAM+++++++    ++PE     
Sbjct: 188 TISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPE--MNS 247

Query: 256 RRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDG 315
           RRL++ +  P+WMD+  RR+L    + V  KP++VVA+DGSG +KTINEAL  +P K + 
Sbjct: 248 RRLLSQE-FPSWMDARARRLLNAPMSEV--KPDIVVAQDGSGQYKTINEALNFVPKKKNT 307

Query: 316 RYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEG 375
            +V+++KEGIY+E V + + M ++   GDG  K++ISGSK++ DG+ T++TAT   +G+ 
Sbjct: 308 TFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDH 367

Query: 376 FLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGT 435
           F+ + + F NTAG  KHQAVA RV AD +IF NC+F+GYQDTLYA +HRQFYR C I+GT
Sbjct: 368 FIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGT 427

Query: 436 IDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPV 495
           IDF+FGDAAA+FQNC +++RKPL NQ   +TA GR D  E+TG VLQ C I+ +     V
Sbjct: 428 IDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAV 487

Query: 496 KSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKT 555
           K Q K+YLGRPWKE+SRTI+M + I D + P+GW PW G+F L TL+Y+E  N GPGA  
Sbjct: 488 KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAI 547

Query: 556 DARVKWPGYKVIDKDEAAKFTIGTFLELD-WIESSSAPVHVGLF 595
             RV WPG K +  +E  KFT   +++ D WI     P  +GLF
Sbjct: 548 TKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563

BLAST of ClCG11G000200 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 248/578 (42.91%), Postives = 357/578 (61.76%), Query Frame = 0

Query: 22  RKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTKQVSRIE 81
           RKR  I +  +SV+L+ +  AV V VS +      +GDSK              +V+   
Sbjct: 11  RKRRYIVITISSVLLISMVVAVTVGVSLNK----HDGDSKGKA-----------EVNASV 70

Query: 82  KMITMICNSTDYKGKCESTL-KDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGFN 141
           K +  +C  TDY+  CE TL K+G +T    +DP +L+K A +    ++  A KK+    
Sbjct: 71  KAVKDVCAPTDYRKTCEDTLIKNGKNT----TDPMELVKTAFNVTMKQITDAAKKSQTIM 130

Query: 142 FATPEEKG--AFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVMSYH 201
               + +   A + CK L++ A++EL  S  E+ K     L  +   +L  WLSA +S+ 
Sbjct: 131 ELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEF-HLLDEALINLRIWLSAAISHE 190

Query: 202 ETCVDGF--PEGKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATRRRLMA 261
           ETC++GF   +G     M+KA+K   ELT N LA+IS++++F    ++P      RRL+A
Sbjct: 191 ETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIP--GLNSRRLLA 250

Query: 262 NDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDGRYVIY 321
            +G P+W+D   R++L+ A+A    KP++VVA+DGSG +KTINEAL  +P K +  +V++
Sbjct: 251 -EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVH 310

Query: 322 VKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEGFLGQG 381
           +K G+Y+E V + K M+++   GDG  K++ISG+KN+ DG+ T++TAT   +G  F+ + 
Sbjct: 311 IKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKN 370

Query: 382 MGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIF 441
           +GF NTAG  KHQAVA RVQ+D +IF NCRF+GYQDTLY  +HRQF+R C I+GTIDF+F
Sbjct: 371 IGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLF 430

Query: 442 GDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKSQFK 501
           GDAAA+FQNC +++RKPL NQ   +TA GR D  E+TG V Q C I  +     VK   K
Sbjct: 431 GDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSK 490

Query: 502 SYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTDARVK 561
           +YLGRPWKE+SRTI+M + I D + P GW PW GDF LKTL+Y+E  N GPG+    RV 
Sbjct: 491 AYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVT 550

Query: 562 WPGYKVIDKDEAAKFTIGTFLE-LDWIESSSAPVHVGL 594
           W G K + +++  KFT   +++  DWI     P   GL
Sbjct: 551 WAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of ClCG11G000200 vs. ExPASy TrEMBL
Match: A0A5D3BLU5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2460G00020 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 581/595 (97.65%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDS 60
           MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILL+VAAAVFVQVSSSSKSTTKNGDS
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGDS 60

Query: 61  KSS-GNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIK 120
            SS GNSK+P+PP+TKQVSR+EKMITMICNSTDYKGKCESTLKDG+HTDP+SSDPKDLIK
Sbjct: 61  GSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLIK 120

Query: 121 LAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180
           LAISAAA EVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180

Query: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVASFF 240
           TAKTTPDLNNWLSAVMSYHETCVDGFPEGKM++EMEK VKAGKELTSNSLAMISQVASFF
Sbjct: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 STFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTIN 300
           STFEMPEGAA+RRRLMA  GVPTWM+SNERRMLKGA+AG KPKPNVVVAKDGSG+FKTIN
Sbjct: 241 STFEMPEGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 EALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRT 360
           EALA MPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISGSKNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420
           FQTATFVALGEGFLGQ +GFRN AGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCNMM+RKPLDNQQNIVTAQGRTDKHETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480

Query: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYY 540
           CKILPDKTLE VKSQFKSYLGRPWKEFSRTIVMES IED+IHPDGWM W+GDFALKTLYY
Sbjct: 481 CKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKTLYY 540

Query: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF 595
           AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES+SAPVHVGLF
Sbjct: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of ClCG11G000200 vs. ExPASy TrEMBL
Match: A0A1S3CCA7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499351 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 581/595 (97.65%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDS 60
           MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILL+VAAAVFVQVSSSSKSTTKNGDS
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGDS 60

Query: 61  KSS-GNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIK 120
            SS GNSK+P+PP+TKQVSR+EKMITMICNSTDYKGKCESTLKDG+HTDP+SSDPKDLIK
Sbjct: 61  GSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLIK 120

Query: 121 LAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180
           LAISAAA EVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180

Query: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVASFF 240
           TAKTTPDLNNWLSAVMSYHETCVDGFPEGKM++EMEK VKAGKELTSNSLAMISQVASFF
Sbjct: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 STFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTIN 300
           STFEMPEGAA+RRRLMA  GVPTWM+SNERRMLKGA+AG KPKPNVVVAKDGSG+FKTIN
Sbjct: 241 STFEMPEGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 EALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRT 360
           EALA MPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISGSKNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420
           FQTATFVALGEGFLGQ +GFRN AGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCNMM+RKPLDNQQNIVTAQGRTDKHETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480

Query: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYY 540
           CKILPDKTLE VKSQFKSYLGRPWKEFSRTIVMES IED+IHPDGWM W+GDFALKTLYY
Sbjct: 481 CKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKTLYY 540

Query: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF 595
           AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES+SAPVHVGLF
Sbjct: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of ClCG11G000200 vs. ExPASy TrEMBL
Match: A0A0A0KX33 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G630740 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 554/595 (93.11%), Postives = 578/595 (97.14%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDS 60
           MVFQDFDQISERRRAERARKFRKRV IAVVSASVILL+VAAAVFVQV SSSKSTTKN DS
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQV-SSSKSTTKNDDS 60

Query: 61  -KSSGNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIK 120
             SSGNSKQP+PP+TKQVSR+EKMITMICNSTDYKGKCESTLKDGVHTDP+SSDPKDLIK
Sbjct: 61  GSSSGNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKDLIK 120

Query: 121 LAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKL 180
           LAISAAA EVKSAVKKASGFNFATPEEKGAFEDCKVLLEDA+EELEMSMSEV+KKNMGKL
Sbjct: 121 LAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEVNKKNMGKL 180

Query: 181 TAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMISQVASFF 240
           TAKTTP+LNNWLSAVMSYHETCVDGFPEGKM++++EK VKAGKELTSNSLAMISQVASFF
Sbjct: 181 TAKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVASFF 240

Query: 241 STFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTIN 300
           STFEMPEGAA+RRRLM  +GVPTWMD N+RRMLKGA+AG KPKPNVVVAKDGSG+FKTIN
Sbjct: 241 STFEMPEGAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGEFKTIN 300

Query: 301 EALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRT 360
           EALA MPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISGSKNFVDGVRT
Sbjct: 301 EALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVRT 360

Query: 361 FQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAH 420
           FQTATFVALGEGFLGQ +GFRN AGPEKHQAVAARVQADRAIFVNCRFEGYQDTLY QAH
Sbjct: 361 FQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYTQAH 420

Query: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQN 480
           RQFYRSCLITGTIDFIFGDAAAIFQNCNMM+RKPLDNQQN+VTAQGRTDKHETTGIVLQN
Sbjct: 421 RQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTGIVLQN 480

Query: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYY 540
           CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIED+IHPDGWM WEGDFALKTLYY
Sbjct: 481 CKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFALKTLYY 540

Query: 541 AEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHVGLF 595
           AEFNN+GPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES+SAPVHVGLF
Sbjct: 541 AEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 594

BLAST of ClCG11G000200 vs. ExPASy TrEMBL
Match: A0A6J1I3R6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470280 PE=3 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.2e-276
Identity = 486/603 (80.60%), Postives = 534/603 (88.56%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNG-- 60
           MVFQDFDQISERRRAERARKFR RV IAVVS  +IL+ V AAVFVQVS  +KSTT++G  
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVFVVAAVFVQVSPPTKSTTRDGAG 60

Query: 61  -----DSKSSGNSKQPVPPDTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSD 120
                 S+S G+SK     D KQVSR +KMITMICNSTDYKGKCESTLK GV T PDSSD
Sbjct: 61  AGSSSSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKQGVETHPDSSD 120

Query: 121 PKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSK 180
           PKDLIKLAI+A + EV SA+KKAS FNF TPEEKGAFEDCK+LLE+AVEEL+ S+SE+S+
Sbjct: 121 PKDLIKLAIAAVSTEVNSALKKASAFNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSR 180

Query: 181 KNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSLAMIS 240
           K++GKLT KTTPDLNNWLSAVMSYH TCVDGFPEGK +T ME  +KAGKELTSNSLAMIS
Sbjct: 181 KDLGKLTDKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTVMEATLKAGKELTSNSLAMIS 240

Query: 241 QVASFFSTFEMPE--GAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDG 300
           QVA+FFSTFE  +  GAATRRRLMA DG+P+WM SNERRMLKG +A  KP PNVVVAKDG
Sbjct: 241 QVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSNERRMLKGDAADDKPTPNVVVAKDG 300

Query: 301 SGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSK 360
           SG+FKTINEALA MPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISG+K
Sbjct: 301 SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNK 360

Query: 361 NFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQ 420
           N  DGVR FQTA+FVALG+G L +G+GFRNTAGPEK QAVAARVQ DRAIFVNCRFEG+Q
Sbjct: 361 NSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQ 420

Query: 421 DTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHE 480
           DTLYAQAHRQFYRSCLITGT+D IFGDAAAIFQNCN ML+KPLD Q N+VTAQGR+D+HE
Sbjct: 421 DTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHE 480

Query: 481 TTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGD 540
           TT  VLQNCKILPDKTLE VK+QFKSYLGRP  E+SRTIVMES IEDIIHPDGWMPW+GD
Sbjct: 481 TTATVLQNCKILPDKTLEAVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGD 540

Query: 541 FALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSSAPVHV 595
           FALKTLYYAEF+N+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+SSS  VHV
Sbjct: 541 FALKTLYYAEFSNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHV 600

BLAST of ClCG11G000200 vs. ExPASy TrEMBL
Match: A0A6J1KC66 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111492523 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.9e-275
Identity = 488/608 (80.26%), Postives = 533/608 (87.66%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQV------SSSSKST 60
           MVFQDFDQISERRR ERARKFR+RV I VV A+VI+LL+AAAVF+QV      SSSS S 
Sbjct: 1   MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60

Query: 61  TKNGDSKSSGNSKQPVPPDT----KQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPD 120
           T N D KS  + K     DT    K  S  EKMI MICNSTDYK KCES LKDG+  DP 
Sbjct: 61  TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120

Query: 121 SSDPKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSE 180
           SSDPK+LIKLAI+AAA EVKSA+KKA+   F TPEEKGAFEDCKVL+EDAVEELEMSM+ 
Sbjct: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180

Query: 181 VSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEG-KMRTEMEKAVKAGKELTSNSL 240
           VSKKN GKLT K TPDLNNWLSAVMSYHETCVDGFP+G KM+ EMEK  K+GKELTSNS+
Sbjct: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240

Query: 241 AMISQVASFFSTFEMP---EGAATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVV 300
           AMISQVA+FFS FE     EGAATRRRLM  D +P+WMDSNERRMLKGA+AG KP PNVV
Sbjct: 241 AMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVV 300

Query: 301 VAKDGSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSM 360
           VA+DGSG+FKTINEALA MPAKYDGRYVIY+KEGIYEE VVITKKM NVTMYGDGSQKSM
Sbjct: 301 VAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSM 360

Query: 361 ISGSKNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCR 420
           ISG+KNF DGVRTFQTA+FVALG+GF  Q +GFRNTAGPEKHQAVAARVQADRAIF+NCR
Sbjct: 361 ISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCR 420

Query: 421 FEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGR 480
           F+GYQDTLYAQAHR FYRSCLI GTIDFIFGDAAAIFQNCNMM++KPLDNQQNIVTAQGR
Sbjct: 421 FDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGR 480

Query: 481 TDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWM 540
           +D+HETT IVLQ CKIL DKTLEPVKSQFKSYLGRPWKE+SRTIVMESTIEDIIHPDGW+
Sbjct: 481 SDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWL 540

Query: 541 PWEGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESSS 595
            W+GDFALKTLYYAEFNN+GPGAKT+ RVKWPG+KVIDK+EAAKFTIG+FLE+DWI+S+S
Sbjct: 541 AWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTS 600

BLAST of ClCG11G000200 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 625.5 bits (1612), Expect = 4.2e-179
Identity = 317/609 (52.05%), Postives = 415/609 (68.14%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSK-------S 60
           M FQDFD+I ER  AER RKFRKR+ + VVS  V+   +    F  V+  +K       +
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  TTKNGDSKSSGNSKQPVPP----DTKQVSRIEKMITMICNSTDYKGKCESTLKDGVHTDP 120
             K+ DS +   S  P PP     T +  +++K+I  +CNST YK  C++TLK+    D 
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120

Query: 121 DSSDPKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMS 180
             +DP+ L+K AI A  D++    K+         ++K A   CK+L+++A EEL  SM 
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180

Query: 181 EVSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEMEKAVKAGKELTSNSL 240
            ++   +    AK  PDL++WLSAVMSY ETCVDGF EGK++TE+ K   + + LTSNSL
Sbjct: 181 RINDSEVNNF-AKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSL 240

Query: 241 AMISQVASFFSTFEMPEG---AATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVV 300
           AMI  +  + S+    +       R      D + +W+ + ERRMLK        KPN  
Sbjct: 241 AMIKSLDGYLSSVPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKAL-KPNAT 300

Query: 301 VAKDGSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSM 360
           VAKDGSG+F TIN AL  MPAKY GRY IY+K GIY+E+V+I KK  NVTM GDGSQK++
Sbjct: 301 VAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTI 360

Query: 361 ISGSKNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCR 420
           ++G+K+    +RTF TATFVA GEGF+ Q MGFRNTAGPE HQAVA RVQ+DR++F+NCR
Sbjct: 361 VTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCR 420

Query: 421 FEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGR 480
           FEGYQDTLYA  HRQ+YRSC+I GT+DFIFGDAAAIFQNC++ +RK L  Q+N VTAQGR
Sbjct: 421 FEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGR 480

Query: 481 TDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWM 540
            DK +TTG V+ NC + P++ L+PVK+QFKSYLGRPWK  SRT+VMESTIED+I P GW+
Sbjct: 481 VDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWL 540

Query: 541 PW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESS 595
            W E DFA+ TL YAE+ N GP   T ARVKWPG++V++K+EA KFT+G FL+ +WI++ 
Sbjct: 541 RWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGEWIQAI 600

BLAST of ClCG11G000200 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 599.4 bits (1544), Expect = 3.2e-171
Identity = 310/620 (50.00%), Postives = 412/620 (66.45%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLLVAAAVFVQVS-------SSSKS 60
           M FQDFD+I ER  A R RKFRKR+ +  VS  V++  +    F  V+          + 
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  TTKNGDSKSSGNS-------KQPVPPDTKQVSRI-----------EKMITMICNSTDYKG 120
             KN +   SGN+       K P PP   Q + +           +K+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGFNFATPEEKGAFEDCKV 180
            CE TLK+         +P   +K AI A  +++   ++K         ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMRTEME 240
           L+EDA EE   S+++++   +     K  PDL +WLSAVMSY ETC+DGF EG +++E++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 KAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATRRRLMANDGVPTWMDSNERRMLKGA 300
            +V + + LTSNSLA+I       S    P      R L+  D +P+W+ +++RRML+  
Sbjct: 241 TSVNSSQVLTSNSLALIKTFTENLS----PVMKVVERHLL--DDIPSWVSNDDRRMLRAV 300

Query: 301 SAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDGRYVIYVKEGIYEETVVITKKMANV 360
                 KPN  VAKDGSGDF TIN+AL  MP KY+GRY+IYVK+GIY+E V + KK AN+
Sbjct: 301 DVKAL-KPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANL 360

Query: 361 TMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEGFLGQGMGFRNTAGPEKHQAVAARV 420
           TM GDGSQK++++G+K+    +RTF TATFVA GEGF+ Q MGFRNTAGPE HQAVA RV
Sbjct: 361 TMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRV 420

Query: 421 QADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMLRKPLD 480
           Q+DR+IF+NCRFEGYQDTLYA  HRQ+YRSC+I GTIDFIFGDAAAIFQNCN+ +RK L 
Sbjct: 421 QSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLP 480

Query: 481 NQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMEST 540
            Q+N VTAQGR DK +TTG V+ NCKI  ++ L+PVK ++KSYLGRPWK +SRTI+MES 
Sbjct: 481 GQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESK 540

Query: 541 IEDIIHPDGWMPW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIG 595
           IE++I P GW+ W E DFA+ TLYYAE+NN+G    T +RVKWPG+KVI+K+EA  +T+G
Sbjct: 541 IENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVG 600

BLAST of ClCG11G000200 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 468.4 bits (1204), Expect = 8.6e-132
Identity = 254/582 (43.64%), Postives = 360/582 (61.86%), Query Frame = 0

Query: 20  KFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTKQVSR 79
           K +K +   V++A ++L++VA  +          TT    S+++ +S++ VP    Q+  
Sbjct: 8   KKKKCIIAGVITALLVLMVVAVGI----------TT----SRNTSHSEKIVP---VQIKT 67

Query: 80  IEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGF 139
               +  +C  TDYK  C ++L   +   PDS+ P DLIKL  +     ++ ++KKAS  
Sbjct: 68  ATTAVEAVCAPTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVE 127

Query: 140 NFA----TPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVM 199
             A      + KGA E C+ L+ DA ++L+  +      ++ ++      DL  WLS  +
Sbjct: 128 LTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQI-EDFVEDLRVWLSGSI 187

Query: 200 SYHETCVDGFPE--GKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPE-----GA 259
           +Y +TC+D F E   K+  +M+K  K  +ELTSN LAMI+ +++    F +       G 
Sbjct: 188 AYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGK 247

Query: 260 ATRRRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAK 319
             R+ L A DG+P+W+  N RR++     GV  K NVVVA DGSG +KTINEAL  +P  
Sbjct: 248 YARKLLSAEDGIPSWVGPNTRRLM-ATKGGV--KANVVVAHDGSGQYKTINEALNAVPKA 307

Query: 320 YDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDG-VRTFQTATFVA 379
               +VIY+K+G+Y E V +TKKM +VT  GDG  K+ I+GS N+  G V+T+ TAT   
Sbjct: 308 NQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAI 367

Query: 380 LGEGFLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCL 439
            G+ F  + +GF NTAGPE HQAVA RV AD A+F NC+ +GYQDTLY  +HRQF+R C 
Sbjct: 368 NGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCT 427

Query: 440 ITGTIDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKT 499
           ++GT+DFIFGD   + QNCN+++RKP+ +Q  ++TAQGR+DK E+TG+VLQNC I  +  
Sbjct: 428 VSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPA 487

Query: 500 LEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGP 559
             PVKS  K+YLGRPWKEFSRTI+M +TI+D+I P GW+PW GDFAL TLYYAE+ N GP
Sbjct: 488 YIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGP 547

Query: 560 GAKTDARVKWPGYKVIDKDEAAKFTIGTFLELD-WIESSSAP 589
           G+    RVKWPG K +   +A +FT   FL  + WI  +  P
Sbjct: 548 GSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVP 565

BLAST of ClCG11G000200 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 465.3 bits (1196), Expect = 7.3e-131
Identity = 248/584 (42.47%), Postives = 361/584 (61.82%), Query Frame = 0

Query: 16  ERARKFRKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTK 75
           E A++ ++ V I++ S  +I ++VA  + V V+ S  +    GD               +
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNA----GD---------------E 67

Query: 76  QVSRIEKMITMICNSTDYKGKCESTLKDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKK 135
           +++   K I  +C  TDYK  CE TL+       D+SDP +L+K A +A   ++    KK
Sbjct: 68  EITTSVKAIKDVCAPTDYKETCEDTLRKDA---KDTSDPLELVKTAFNATMKQISDVAKK 127

Query: 136 ASGF--NFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSA 195
           +         P  K A + CK L++ A+ EL  S  E+ K    K+  +    L  WLSA
Sbjct: 128 SQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVD-EALVKLRIWLSA 187

Query: 196 VMSYHETCVDGF--PEGKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATR 255
            +S+ +TC+DGF   +G     ++KA+K   +LT N LAM+++++++    ++PE     
Sbjct: 188 TISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPE--MNS 247

Query: 256 RRLMANDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDG 315
           RRL++ +  P+WMD+  RR+L    + V  KP++VVA+DGSG +KTINEAL  +P K + 
Sbjct: 248 RRLLSQE-FPSWMDARARRLLNAPMSEV--KPDIVVAQDGSGQYKTINEALNFVPKKKNT 307

Query: 316 RYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEG 375
            +V+++KEGIY+E V + + M ++   GDG  K++ISGSK++ DG+ T++TAT   +G+ 
Sbjct: 308 TFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDH 367

Query: 376 FLGQGMGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGT 435
           F+ + + F NTAG  KHQAVA RV AD +IF NC+F+GYQDTLYA +HRQFYR C I+GT
Sbjct: 368 FIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGT 427

Query: 436 IDFIFGDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPV 495
           IDF+FGDAAA+FQNC +++RKPL NQ   +TA GR D  E+TG VLQ C I+ +     V
Sbjct: 428 IDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAV 487

Query: 496 KSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKT 555
           K Q K+YLGRPWKE+SRTI+M + I D + P+GW PW G+F L TL+Y+E  N GPGA  
Sbjct: 488 KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAI 547

Query: 556 DARVKWPGYKVIDKDEAAKFTIGTFLELD-WIESSSAPVHVGLF 595
             RV WPG K +  +E  KFT   +++ D WI     P  +GLF
Sbjct: 548 TKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563

BLAST of ClCG11G000200 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 461.8 bits (1187), Expect = 8.1e-130
Identity = 248/578 (42.91%), Postives = 357/578 (61.76%), Query Frame = 0

Query: 22  RKRVTIAVVSASVILLLVAAAVFVQVSSSSKSTTKNGDSKSSGNSKQPVPPDTKQVSRIE 81
           RKR  I +  +SV+L+ +  AV V VS +      +GDSK              +V+   
Sbjct: 11  RKRRYIVITISSVLLISMVVAVTVGVSLNK----HDGDSKGKA-----------EVNASV 70

Query: 82  KMITMICNSTDYKGKCESTL-KDGVHTDPDSSDPKDLIKLAISAAADEVKSAVKKASGFN 141
           K +  +C  TDY+  CE TL K+G +T    +DP +L+K A +    ++  A KK+    
Sbjct: 71  KAVKDVCAPTDYRKTCEDTLIKNGKNT----TDPMELVKTAFNVTMKQITDAAKKSQTIM 130

Query: 142 FATPEEKG--AFEDCKVLLEDAVEELEMSMSEVSKKNMGKLTAKTTPDLNNWLSAVMSYH 201
               + +   A + CK L++ A++EL  S  E+ K     L  +   +L  WLSA +S+ 
Sbjct: 131 ELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEF-HLLDEALINLRIWLSAAISHE 190

Query: 202 ETCVDGF--PEGKMRTEMEKAVKAGKELTSNSLAMISQVASFFSTFEMPEGAATRRRLMA 261
           ETC++GF   +G     M+KA+K   ELT N LA+IS++++F    ++P      RRL+A
Sbjct: 191 ETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIP--GLNSRRLLA 250

Query: 262 NDGVPTWMDSNERRMLKGASAGVKPKPNVVVAKDGSGDFKTINEALAKMPAKYDGRYVIY 321
            +G P+W+D   R++L+ A+A    KP++VVA+DGSG +KTINEAL  +P K +  +V++
Sbjct: 251 -EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVH 310

Query: 322 VKEGIYEETVVITKKMANVTMYGDGSQKSMISGSKNFVDGVRTFQTATFVALGEGFLGQG 381
           +K G+Y+E V + K M+++   GDG  K++ISG+KN+ DG+ T++TAT   +G  F+ + 
Sbjct: 311 IKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKN 370

Query: 382 MGFRNTAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYAQAHRQFYRSCLITGTIDFIF 441
           +GF NTAG  KHQAVA RVQ+D +IF NCRF+GYQDTLY  +HRQF+R C I+GTIDF+F
Sbjct: 371 IGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLF 430

Query: 442 GDAAAIFQNCNMMLRKPLDNQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKSQFK 501
           GDAAA+FQNC +++RKPL NQ   +TA GR D  E+TG V Q C I  +     VK   K
Sbjct: 431 GDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSK 490

Query: 502 SYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTDARVK 561
           +YLGRPWKE+SRTI+M + I D + P GW PW GDF LKTL+Y+E  N GPG+    RV 
Sbjct: 491 AYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVT 550

Query: 562 WPGYKVIDKDEAAKFTIGTFLE-LDWIESSSAPVHVGL 594
           W G K + +++  KFT   +++  DWI     P   GL
Sbjct: 551 WAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460558.10.0e+0094.45PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo] >K... [more]
XP_004142361.10.0e+0093.11putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus] >KGN52371.... [more]
XP_038893171.10.0e+0092.63putative pectinesterase/pectinesterase inhibitor 45 [Benincasa hispida][more]
XP_022971606.14.5e-27680.60putative pectinesterase/pectinesterase inhibitor 45 [Cucurbita maxima][more]
KAG6601658.11.7e-27580.96putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q9SMY65.9e-17852.05Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q7Y2014.6e-17050.00Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ211.2e-13043.64Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z81.0e-12942.47Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX861.1e-12842.91Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5D3BLU50.0e+0094.45Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2460G0002... [more]
A0A1S3CCA70.0e+0094.45Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499351 PE=3 SV=1[more]
A0A0A0KX330.0e+0093.11Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G630740 PE=3 SV=1[more]
A0A6J1I3R62.2e-27680.60Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470280 PE=3 SV=1[more]
A0A6J1KC661.9e-27580.26Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111492523 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G33230.14.2e-17952.05Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.13.2e-17150.00Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.18.6e-13243.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.17.3e-13142.47Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.18.1e-13042.91Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 154..174
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..73
NoneNo IPR availablePANTHERPTHR31707:SF191PECTINESTERASE/PECTINESTERASE INHIBITOR 45-RELATEDcoord: 14..592
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 14..592
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 87..237
e-value: 3.04087E-43
score: 149.896
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 79..231
e-value: 2.5E-38
score: 143.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 83..231
e-value: 5.2E-28
score: 98.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 81..235
e-value: 6.8E-23
score: 78.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 80..236
e-value: 2.2E-33
score: 117.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 82..235
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 284..578
e-value: 3.4E-141
score: 469.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 278..593
e-value: 1.7E-115
score: 387.8
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 428..437
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 280..578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG11G000200.1ClCG11G000200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity