ClCG10G022840 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG10G022840
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	calcium-binding allergen Bet v 3
Location	CG_Chr10: 37171394 .. 37171993 (-)
RNA-Seq Expression	ClCG10G022840
Synteny	ClCG10G022840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCGGTGGCCTCAGATGTTCCCCGACCCCGCCTGACAAAGAAGCCGTCGTCTTCGTCTTCCTCGTTCCAGCTTCGGTGCCCGAGCCTGAACTCCCTTCGGCTGCGTCGGATATTTGACATGTTCGACAAGAACGGGGACGGCATGATCACGACGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCCGATGAATCGGAGCTGGATTCGACGGTGAACTCCTTCATCGAACCGGGAAATGTGGGTCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGGCCTTAAATGACACCTATTTTGGGCCGGGGATGGTTGAGGAGGAGGAGAAAAGGGAAACAAGCATGTCGTCGATGTCGCAGGAAGAGTCGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCCGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGATATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

mRNA sequence

ATGGAAGCGGTGGCCTCAGATGTTCCCCGACCCCGCCTGACAAAGAAGCCGTCGTCTTCGTCTTCCTCGTTCCAGCTTCGGTGCCCGAGCCTGAACTCCCTTCGGCTGCGTCGGATATTTGACATGTTCGACAAGAACGGGGACGGCATGATCACGACGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCCGATGAATCGGAGCTGGATTCGACGGTGAACTCCTTCATCGAACCGGGAAATGTGGGTCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGGCCTTAAATGACACCTATTTTGGGCCGGGGATGGTTGAGGAGGAGGAGAAAAGGGAAACAAGCATGTCGTCGATGTCGCAGGAAGAGTCGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCCGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGATATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

Coding sequence (CDS)

ATGGAAGCGGTGGCCTCAGATGTTCCCCGACCCCGCCTGACAAAGAAGCCGTCGTCTTCGTCTTCCTCGTTCCAGCTTCGGTGCCCGAGCCTGAACTCCCTTCGGCTGCGTCGGATATTTGACATGTTCGACAAGAACGGGGACGGCATGATCACGACGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCCGATGAATCGGAGCTGGATTCGACGGTGAACTCCTTCATCGAACCGGGAAATGTGGGTCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGGCCTTAAATGACACCTATTTTGGGCCGGGGATGGTTGAGGAGGAGGAGAAAAGGGAAACAAGCATGTCGTCGATGTCGCAGGAAGAGTCGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCCGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGATATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

Protein sequence

MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVIVRSC

Homology

[Close]

BLAST of ClCG10G022840 vs. NCBI nr
Match: XP_008442311.1 (PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa] >TYK24898.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa])

HSP 1 Score: 364.4 bits (934), Expect = 6.3e-97
Identity = 188/199 (94.47%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSSSSSSF+LRCPSLNSLRLRRIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMS 120
           GLLGLDADESELDSTVNSFI+PGNVGLRFEDFAALHQ+LNDTYF   + +E+EKRETSMS
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120

Query: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180
           SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG
Sbjct: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180

Query: 181 RVDFFEFKDMMRSVIVRSC 200
           RVDFFEFKDMMRSVIVRSC
Sbjct: 181 RVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of ClCG10G022840 vs. NCBI nr
Match: XP_004137668.1 (calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein Csa_002212 [Cucumis sativus])

HSP 1 Score: 363.6 bits (932), Expect = 1.1e-96
Identity = 192/202 (95.05%), Postives = 196/202 (97.03%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSS SSSF+LRCPSLNSLRLRRIFDMFDKNGDGMITT+ELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVE---EEEKRET 120
           GLLGLDADESELDSTVNSFIEPGN GLRFEDFAALHQ+LNDTYFG GMVE   E+EKRET
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFG-GMVEDEDEDEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN
Sbjct: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           QDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of ClCG10G022840 vs. NCBI nr
Match: XP_022982604.1 (calcium-binding allergen Bet v 3-like [Cucurbita maxima])

HSP 1 Score: 348.6 bits (893), Expect = 3.6e-92
Identity = 181/202 (89.60%), Postives = 191/202 (94.55%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSS SF+LRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEE---EEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ+LNDTYFG  M +E   EEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of ClCG10G022840 vs. NCBI nr
Match: XP_023527056.1 (calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 348.2 bits (892), Expect = 4.7e-92
Identity = 181/202 (89.60%), Postives = 191/202 (94.55%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSSSS SF+LRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 70  MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 129

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEE---EEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ+LNDTYFG  + +E   EEKRET
Sbjct: 130 GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPVEDEEEKEEKRET 189

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 190 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 249

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 250 RDGRVDFFEFKDMMRSVIVRSC 271

[Close]

BLAST of ClCG10G022840 vs. NCBI nr
Match: KAG6580420.1 (Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 345.1 bits (884), Expect = 4.0e-91
Identity = 180/202 (89.11%), Postives = 190/202 (94.06%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSSSS SF+LRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEE---EEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ+LNDTYF   + +E   EEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFREPVEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of ClCG10G022840 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.8e-61
Identity = 122/185 (65.95%), Postives = 150/185 (81.08%), Query Frame = 0


Query: 15  KKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           +K S S+SSF+LR  SLN+LRLRRIFD+FDKN DG+IT +EL +AL LLGL+ D SEL+S
Sbjct: 20  RKRSLSNSSFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELES 79

Query: 75  TVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSS-MSQEESDLSEAF 134
           TV SF   GN+GL+FEDF +LHQ+LND+YF  G  +E++  E    S +SQEE+D    F
Sbjct: 80  TVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGF 139

Query: 135 RVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRS 194
           +VFDEDGDGYISA ELQ+VL KL  +EG+EIDRV+KMI+SVD N+DGRVDFFEFKDMMRS
Sbjct: 140 KVFDEDGDGYISARELQMVLGKLGFSEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMMRS 199

Query: 195 VIVRS 199
           V+VRS
Sbjct: 200 VLVRS 204

[Close]

BLAST of ClCG10G022840 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 2.4e-59
Identity = 118/184 (64.13%), Postives = 149/184 (80.98%), Query Frame = 0


Query: 15  KKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK +  SSSF+LR PSLN+LRL+RIFD+FDKNGDG IT EEL QAL  LGL+AD S+L S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFR 134
           TV S+I+PGN GL F+DF++LH+ L+D++FG      E   E   SS ++ ESDL+EAF+
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGE--NEDDPSSAAENESDLAEAFK 128

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 194
           VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVDRNQDGRVDFFEFK+MMR+V
Sbjct: 129 VFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRTV 188

Query: 195 IVRS 199
           ++ S
Sbjct: 189 VIPS 190

[Close]

BLAST of ClCG10G022840 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.8e-54
Identity = 117/184 (63.59%), Postives = 141/184 (76.63%), Query Frame = 0


Query: 15  KKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           K   SSSSSF+LR PSLNS+RLRRIFD+FD+N D +I+ EEL QAL LLGLDAD SE++S
Sbjct: 20  KLKPSSSSSFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIES 79

Query: 75  TVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFR 134
            V   I+P N GLRFEDF  LH++LND +FG     + E +       +Q+ESDL EAF 
Sbjct: 80  MVKLHIKPENTGLRFEDFETLHRSLNDVFFG----SKCEDKLGLNPDPAQDESDLKEAFD 139

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 194
           VFDE+GDG+ISA ELQVVL KL L EG+EIDRV+ MI SV+++ DGRVDFFEFKDMMR+V
Sbjct: 140 VFDENGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMMRTV 199

Query: 195 IVRS 199
           IV S
Sbjct: 200 IVPS 199

[Close]

BLAST of ClCG10G022840 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.4e-54
Identity = 116/184 (63.04%), Postives = 139/184 (75.54%), Query Frame = 0


Query: 15  KKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK S  SSSF+LR PSLN+LRL R+FD+FDKN DG IT EEL QAL  LGLDAD S+L S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFR 134
           TV+SFI+P   GLRF+DFAALH+ L++++FG          E S    S  ESDL EAF 
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFG---------GEGSCCDGS-PESDLEEAFN 127

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 194
           VFDEDGDG+ISA ELQ VL+KL L E  EI++V+KMI+SVD N DGRVDFFEFK+MM++V
Sbjct: 128 VFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTV 181

Query: 195 IVRS 199
           +V S
Sbjct: 188 VVPS 181

[Close]

BLAST of ClCG10G022840 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.8e-47
Identity = 105/187 (56.15%), Postives = 130/187 (69.52%), Query Frame = 0


Query: 10  RPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADE 69
           +P L++KP   S SF+LR  SLN+LRLRR+FD+FD+NGDG IT +E+  AL  LGL AD 
Sbjct: 9   KPSLSRKP---SPSFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASALDALGLGADR 68

Query: 70  SELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDL 129
           + L++TV  +I  G  GLRF DF ALH+AL D  FGP  VEEE   E        +E D+
Sbjct: 69  AGLEATVGGYIPAGAAGLRFGDFEALHRALGDALFGP--VEEE---EPGKQGEDDDEGDM 128

Query: 130 SEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKD 189
            EAFRVFDEDGDG+ISA ELQ VL+KL L E   +  VQ+MI +VDR+ DGRVDF EFK 
Sbjct: 129 KEAFRVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGRVDFGEFKC 187

Query: 190 MMRSVIV 197
           MM+ + V
Sbjct: 189 MMQGITV 187

[Close]

BLAST of ClCG10G022840 vs. ExPASy TrEMBL
Match: A0A5A7TQ56 (Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00090 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.1e-97
Identity = 188/199 (94.47%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSSSSSSF+LRCPSLNSLRLRRIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMS 120
           GLLGLDADESELDSTVNSFI+PGNVGLRFEDFAALHQ+LNDTYF   + +E+EKRETSMS
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120

Query: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180
           SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG
Sbjct: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180

Query: 181 RVDFFEFKDMMRSVIVRSC 200
           RVDFFEFKDMMRSVIVRSC
Sbjct: 181 RVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of ClCG10G022840 vs. ExPASy TrEMBL
Match: A0A1S3B620 (calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.1e-97
Identity = 188/199 (94.47%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSSSSSSF+LRCPSLNSLRLRRIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMS 120
           GLLGLDADESELDSTVNSFI+PGNVGLRFEDFAALHQ+LNDTYF   + +E+EKRETSMS
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKRETSMS 120

Query: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180
           SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG
Sbjct: 121 SMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDG 180

Query: 181 RVDFFEFKDMMRSVIVRSC 200
           RVDFFEFKDMMRSVIVRSC
Sbjct: 181 RVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of ClCG10G022840 vs. ExPASy TrEMBL
Match: A0A0A0LA10 (Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.2e-97
Identity = 192/202 (95.05%), Postives = 196/202 (97.03%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSS SSSF+LRCPSLNSLRLRRIFDMFDKNGDGMITT+ELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVE---EEEKRET 120
           GLLGLDADESELDSTVNSFIEPGN GLRFEDFAALHQ+LNDTYFG GMVE   E+EKRET
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFG-GMVEDEDEDEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN
Sbjct: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           QDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of ClCG10G022840 vs. ExPASy TrEMBL
Match: A0A6J1J521 (calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481430 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.7e-92
Identity = 181/202 (89.60%), Postives = 191/202 (94.55%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSS SF+LRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEE---EEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ+LNDTYFG  M +E   EEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of ClCG10G022840 vs. ExPASy TrEMBL
Match: A0A6J1F9I1 (calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442046 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.3e-91
Identity = 180/202 (89.11%), Postives = 189/202 (93.56%), Query Frame = 0


Query: 1   MEAVASDVPRPRLTKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAVASDVPRPRLTKKPSSSS SF+LRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEE---EEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ LNDTYF   + +E   EEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 200
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of ClCG10G022840 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-60
Identity = 118/184 (64.13%), Postives = 149/184 (80.98%), Query Frame = 0


Query: 15  KKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK +  SSSF+LR PSLN+LRL+RIFD+FDKNGDG IT EEL QAL  LGL+AD S+L S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFR 134
           TV S+I+PGN GL F+DF++LH+ L+D++FG      E   E   SS ++ ESDL+EAF+
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGE--NEDDPSSAAENESDLAEAFK 128

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 194
           VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVDRNQDGRVDFFEFK+MMR+V
Sbjct: 129 VFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRTV 188

Query: 195 IVRS 199
           ++ S
Sbjct: 189 VIPS 190

[Close]

BLAST of ClCG10G022840 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 213.4 bits (542), Expect = 1.7e-55
Identity = 116/184 (63.04%), Postives = 139/184 (75.54%), Query Frame = 0


Query: 15  KKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK S  SSSF+LR PSLN+LRL R+FD+FDKN DG IT EEL QAL  LGLDAD S+L S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFR 134
           TV+SFI+P   GLRF+DFAALH+ L++++FG          E S    S  ESDL EAF 
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFG---------GEGSCCDGS-PESDLEEAFN 127

Query: 135 VFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSV 194
           VFDEDGDG+ISA ELQ VL+KL L E  EI++V+KMI+SVD N DGRVDFFEFK+MM++V
Sbjct: 128 VFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTV 181

Query: 195 IVRS 199
           +V S
Sbjct: 188 VVPS 181

[Close]

BLAST of ClCG10G022840 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 114.8 bits (286), Expect = 8.1e-26
Identity = 66/179 (36.87%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 14  TKKPSSSSSSFQLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELD 73
           T  PS SSSS   +   ++   L+R+F MFDKNGDG IT EEL  +L  LG+   + +L 
Sbjct: 46  TMLPSPSSSSAPTK--RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLT 105

Query: 74  STVNSFIEPGNVGLRFEDFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAF 133
             ++     G+  +  ++F +L+ ++ D +   G  EEE               D+ +AF
Sbjct: 106 QMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEE---------------DMKDAF 165

Query: 134 RVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMR 193
            VFD+DGDG+I+  EL+ V+  L L +G  +D  +KMI+ VD + DGRV++ EF  MM+
Sbjct: 166 NVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of ClCG10G022840 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 109.4 bits (272), Expect = 3.4e-24
Identity = 61/162 (37.65%), Postives = 94/162 (58.02%), Query Frame = 0


Query: 31  LNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDSTVNSFIEPGNVGLRFE 90
           ++S  L R+F MFDK+GDG ITT+EL ++   LG+   E EL   +      G+  +  E
Sbjct: 1   MDSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIE 60

Query: 91  DFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQ 150
           +F  L++ +        MVE+E++           E D+ EAF VFD +GDG+I+  EL+
Sbjct: 61  EFGELYKTI--------MVEDEDE---------VGEEDMKEAFNVFDRNGDGFITVDELK 120

Query: 151 VVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMR 193
            VL  L L +G  ++  +KMI+ VD + DGRV++ EF+ MM+
Sbjct: 121 AVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

[Close]

BLAST of ClCG10G022840 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-23
Identity = 61/162 (37.65%), Postives = 92/162 (56.79%), Query Frame = 0


Query: 31  LNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDSTVNSFIEPGNVGLRFE 90
           ++   L+R+F MFDKNGDG IT +EL + L  LG+   + EL   +      G+  +  +
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 91  DFAALHQALNDTYFGPGMVEEEEKRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQ 150
           +F  L++ + D        EE+E           EE D+ EAF VFD++GDG+I+  EL+
Sbjct: 61  EFGELYKTIMD--------EEDE-----------EEEDMKEAFNVFDQNGDGFITVDELK 120

Query: 151 VVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMR 193
            VL  L L +G  +D  +KMI  VD + DGRV++ EF+ MM+
Sbjct: 121 AVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

The following BLAST results are available for this feature:

BLAST of ClCG10G022840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442311.1	6.3e-97	94.47	PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium...	[more]
XP_004137668.1	1.1e-96	95.05	calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein...	[more]
XP_022982604.1	3.6e-92	89.60	calcium-binding allergen Bet v 3-like [Cucurbita maxima]	[more]
XP_023527056.1	4.7e-92	89.60	calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6580420.1	4.0e-91	89.11	Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of ClCG10G022840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43187	5.8e-61	65.95	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q9SVG9	2.4e-59	64.13	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q09011	1.8e-54	63.59	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9FI19	2.4e-54	63.04	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q10LX4	2.8e-47	56.15	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of ClCG10G022840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TQ56	3.1e-97	94.47	Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B620	3.1e-97	94.47	calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV...	[more]
A0A0A0LA10	5.2e-97	95.05	Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=...	[more]
A0A6J1J521	1.7e-92	89.60	calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC11148143...	[more]
A0A6J1F9I1	4.3e-91	89.11	calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442...	[more]

BLAST of ClCG10G022840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20780.1	1.7e-60	64.13	calmodulin like 42	[more]
AT5G44460.1	1.7e-55	63.04	calmodulin like 43	[more]
AT2G43290.1	8.1e-26	36.87	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	3.4e-24	37.65	EF hand calcium-binding protein family	[more]
AT1G05990.1	1.7e-23	37.65	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 35..63 e-value: 2.4E-6 score: 37.1 coord: 166..194 e-value: 9.0E-6 score: 35.2 coord: 128..156 e-value: 2.8E-6 score: 36.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 127..192 e-value: 3.8E-13 score: 49.7
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 35..64 e-value: 1.7E-8 score: 33.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 124..159 score: 14.90394
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 162..197 score: 13.592857
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 31..66 score: 15.712908
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 128..192 e-value: 5.03684E-16 score: 67.1877
None	No IPR available	GENE3D	1.10.238.10		coord: 33..101 e-value: 7.7E-14 score: 53.5
None	No IPR available	GENE3D	1.10.238.10		coord: 119..160 e-value: 1.8E-11 score: 45.4
None	No IPR available	GENE3D	1.10.238.10		coord: 161..198 e-value: 9.2E-7 score: 30.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..198
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 6..198
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 175..187
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 44..56
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 137..149
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 35..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG10G022840.1	ClCG10G022840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding