ClCG10G015230 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG10G015230
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Calcium-binding EF hand family protein
Location	CG_Chr10: 29746802 .. 29747951 (-)
RNA-Seq Expression	ClCG10G015230
Synteny	ClCG10G015230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAATAATAATAATAATAATAATAATAATAAAACCAAAACCAAAACCAAAACCAAGCTTGAAAAATGTATTGAAGTTTCCTAACAACATCATCTCATGTTGAACATTATTCTATCTTAATTGCACATCTTCTTCTACACATATCCCAGCAATAAGCTAGTAAATTTCAAATACCAAACCTCCAAATCTGACCAAATATCCCATGTTACTTTCTTTATATATATATACACACACACACACTTTGATCTTCATCTCCAATTCACCACATTAATTATCAATCCACACAGAAACTCTGTATCCATGTTCTTTAGGTTAGAGTACTATTTAATCACAATGCAAACCCGATTATCCGACACCAACGTGCAGCTCGTCCCCACGCCACTGGTGTTCGTCGATCGGATATCGATTTTACCAATTTTTTTCATCTTTGCAGTCAAATTACATGACCTGATCAAATCTCTACCTTTGTCAATTTTTCAATCACCCTCCAAAGAAAAGACTTCGTCACCAAGTAATTCTACTTCACAATCTGATGATCAGGTCATCAATACTCAACAAAAGAATTGCTTATTGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGCATCCCAGAAGAAGAAGATGATGTTGAAGAATTTGGGGAATGTTTTGGAGGGGATGAAATAACAGCTATGTTTGAAGAGAATGAACCAAGCTTTGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCAGAGGAATTGCACAAAGTTTTGGGCCTTCTTGGCTCTAATAAAGGCATATTCATCCATGACTGCAAAAGAATGATAGCCAGATTTGATCACAATAATGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTGAATTTTCTATTATTTGTATCTACATGTTTGACATTTTTATTGACTATTTCATATTTTCGATGAGTTCCATGCCGCGGTAAGAAATCAATCAATATTTTTACTAATATCATATAAAAATTTTTATGTCATAATAAAAATTTTTGAATACTAACATAGACATAGTATTGATAGTAAGAAATTTTAAATTATTTATAATTTTTTAATGCACTTTCTCTACTTTGTTT

mRNA sequence

AATAATAATAATAATAATAATAATAATAATAAAACCAAAACCAAAACCAAAACCAAGCTTGAAAAATGTATTGAAGTTTCCTAACAACATCATCTCATGTTGAACATTATTCTATCTTAATTGCACATCTTCTTCTACACATATCCCAGCAATAAGCTAGTAAATTTCAAATACCAAACCTCCAAATCTGACCAAATATCCCATGTTACTTTCTTTATATATATATACACACACACACACTTTGATCTTCATCTCCAATTCACCACATTAATTATCAATCCACACAGAAACTCTGTATCCATGTTCTTTAGGTTAGAGTACTATTTAATCACAATGCAAACCCGATTATCCGACACCAACGTGCAGCTCGTCCCCACGCCACTGGTGTTCGTCGATCGGATATCGATTTTACCAATTTTTTTCATCTTTGCAGTCAAATTACATGACCTGATCAAATCTCTACCTTTGTCAATTTTTCAATCACCCTCCAAAGAAAAGACTTCGTCACCAAGTAATTCTACTTCACAATCTGATGATCAGGTCATCAATACTCAACAAAAGAATTGCTTATTGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGCATCCCAGAAGAAGAAGATGATGTTGAAGAATTTGGGGAATGTTTTGGAGGGGATGAAATAACAGCTATGTTTGAAGAGAATGAACCAAGCTTTGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCAGAGGAATTGCACAAAGTTTTGGGCCTTCTTGGCTCTAATAAAGGCATATTCATCCATGACTGCAAAAGAATGATAGCCAGATTTGATCACAATAATGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTGAATTTTCTATTATTTGTATCTACATGTTTGACATTTTTATTGACTATTTCATATTTTCGATGAGTTCCATGCCGCGGTAAGAAATCAATCAATATTTTTACTAATATCATATAAAAATTTTTATGTCATAATAAAAATTTTTGAATACTAACATAGACATAGTATTGATAGTAAGAAATTTTAAATTATTTATAATTTTTTAATGCACTTTCTCTACTTTGTTT

Coding sequence (CDS)

ATGTTCTTTAGGTTAGAGTACTATTTAATCACAATGCAAACCCGATTATCCGACACCAACGTGCAGCTCGTCCCCACGCCACTGGTGTTCGTCGATCGGATATCGATTTTACCAATTTTTTTCATCTTTGCAGTCAAATTACATGACCTGATCAAATCTCTACCTTTGTCAATTTTTCAATCACCCTCCAAAGAAAAGACTTCGTCACCAAGTAATTCTACTTCACAATCTGATGATCAGGTCATCAATACTCAACAAAAGAATTGCTTATTGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGCATCCCAGAAGAAGAAGATGATGTTGAAGAATTTGGGGAATGTTTTGGAGGGGATGAAATAACAGCTATGTTTGAAGAGAATGAACCAAGCTTTGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCAGAGGAATTGCACAAAGTTTTGGGCCTTCTTGGCTCTAATAAAGGCATATTCATCCATGACTGCAAAAGAATGATAGCCAGATTTGATCACAATAATGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTGA

Protein sequence

MFFRLEYYLITMQTRLSDTNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIFQSPSKEKTSSPSNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNNDGKIDFNEFVKFMEVALS

Homology

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BLAST of ClCG10G015230 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 302.4 bits (773), Expect = 3.1e-78
Identity = 161/197 (81.73%), Postives = 171/197 (86.80%), Query Frame = 0


Query: 12  MQTRLSDTNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIFQSPSKEKTSSPS 71
           MQ RL D NVQL+PTPLVFVDR SILPI F FA ++HDLI S+PLS FQSPSKEK S  S
Sbjct: 1   MQNRLPDVNVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPLSFFQSPSKEKASPQS 60

Query: 72  NSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAMF 131
           N +SQSDDQVI TQQKNCL+SREEVRFVM+KLELFC   + D  EE GECFGG+E+TAMF
Sbjct: 61  NCSSQSDDQVI-TQQKNCLMSREEVRFVMDKLELFC--SKNDGDEEIGECFGGEELTAMF 120

Query: 132 EENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNND 191
           EENEPS EELKQTFNVFDRNRDGFIDAEELH VLGLL SN GIFIHDCKRMIARFDHNND
Sbjct: 121 EENEPSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFDHNND 180

Query: 192 GKIDFNEFVKFMEVALS 209
           GKIDF EFVKFMEVALS
Sbjct: 181 GKIDFTEFVKFMEVALS 193

[Close]

BLAST of ClCG10G015230 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 249.6 bits (636), Expect = 2.4e-62
Identity = 143/210 (68.10%), Postives = 161/210 (76.67%), Query Frame = 0


Query: 1   MFFRLEYYLITMQTRLSD-TNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIF 60
           MFFRLEYYLITM+TRL+D  +VQL P+P VFVDRISILPI F FA ++ +L++SLP  I 
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  QSPSKEKTSSPSNSTSQSDDQVINTQQKNC-LLSREEVRFVMEKLELFCIPEEEDDVEEF 120
           Q  SKEK  S  N +S+S DQ+I T Q+NC  +SREEVRFVMEKLELF   E +    + 
Sbjct: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120

Query: 121 GECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHD 180
           G     + I  MFEENEPS EELKQTFNVFDRNRDGFID  EL  VL LL SNKGIFIHD
Sbjct: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180

Query: 181 CKRMIARFDHNNDGKIDFNEFVKFMEVALS 209
           CK MIARFD NNDGKIDF+EFVKFMEVALS
Sbjct: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

[Close]

BLAST of ClCG10G015230 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 231.5 bits (589), Expect = 6.7e-57
Identity = 138/201 (68.66%), Postives = 157/201 (78.11%), Query Frame = 0


Query: 12  MQTRLSDT--NVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPL-SIFQSP-SKEKT 71
           MQT++++   +VQL P+PLVFVD IS LPI F FA ++H+LI+SLP  SI QS  SKEK 
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  SSPSNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDE- 131
            S SN +SQSDD++I T Q+NC +SREEVRFVMEKLELF   EE DD ++  E    DE 
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFW-REENDDDDDDEEIGASDEI 120

Query: 132 ITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARF 191
           I  MFEENEPS EELKQTFNVFDRN+DGFID  EL  VL LL SNKGIFIHDCK MIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 192 DHNNDGKIDFNEFVKFMEVAL 208
           D NNDGKIDF+EFVKFMEVAL
Sbjct: 181 DLNNDGKIDFHEFVKFMEVAL 200

[Close]

BLAST of ClCG10G015230 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.5 bits (589), Expect = 6.7e-57
Identity = 135/214 (63.08%), Postives = 158/214 (73.83%), Query Frame = 0


Query: 1   MFFRLEYYLITMQTRLSDTNVQLVPT------PLVFVDRISILPIFFIFAVKLHDLIKSL 60
           MFF+LE YL+TM       N+QL+P+      PLVF+DR S      IFA  +HDLI SL
Sbjct: 1   MFFKLE-YLLTMHNPQPTINLQLIPSTTTTTPPLVFIDRAS------IFADTIHDLI-SL 60

Query: 61  PLSIFQSPSKEKTSSPSNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDD 120
             S FQSP KEK ++P +  SQS+       +++ L+SREEV+ VMEKLEL+C  EE+D+
Sbjct: 61  VFSFFQSPPKEK-AAPQSRASQSEG------KEDWLMSREEVKSVMEKLELYCNEEEDDE 120

Query: 121 VEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGI 180
           ++   ECFG DE+  MFEENEPS EELKQTFNVFDRNRDGFIDA+ELH VLGLL SN  I
Sbjct: 121 ID---ECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTI 180

Query: 181 FIHDCKRMIARFDHNNDGKIDFNEFVKFMEVALS 209
           FIHDCK MIARFDHNND KIDFNEFVKFMEVALS
Sbjct: 181 FIHDCKSMIARFDHNNDQKIDFNEFVKFMEVALS 196

[Close]

BLAST of ClCG10G015230 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 229.2 bits (583), Expect = 3.3e-56
Identity = 132/199 (66.33%), Postives = 150/199 (75.38%), Query Frame = 0


Query: 12  MQTRLSD-TNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIFQSPSKEKTSSP 71
           M+TRL+D  +VQL P+P VFVDRISILPI F FA ++ +L++SLP  I Q  SKEK  S 
Sbjct: 1   METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 60

Query: 72  SNSTSQSDDQVINTQQKNC-LLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITA 131
            N +S+S DQ+I T Q+NC  +SREEVRFVMEKLELF   E +    + G     + I  
Sbjct: 61  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 120

Query: 132 MFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHN 191
           MFEENEPS EELKQTFNVFDRNRDGFID  EL  VL LL SNKGIFIHDCK MIARFD N
Sbjct: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180

Query: 192 NDGKIDFNEFVKFMEVALS 209
           NDGKIDF+EFVKFMEVALS
Sbjct: 181 NDGKIDFHEFVKFMEVALS 199

[Close]

BLAST of ClCG10G015230 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.5e-23
Identity = 62/143 (43.36%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 64  KEKTSSPSNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFG 123
           +++T +        DD   +    +  +SREE   VM  L LF      DD  +  E + 
Sbjct: 56  QQETLTKQEDDDDDDDDDDDDDDDDIDISREEAEMVMRSLGLF----YNDD--QLQEQYS 115

Query: 124 GDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMI 183
             E++++FEE E S EE+KQ F+VFD N+DGFIDA EL +VL +LG  +G ++ +C  MI
Sbjct: 116 AKEVSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMI 175

Query: 184 ARFDHNNDGKIDFNEFVKFMEVA 207
              D N DGKIDFNEFVKFME +
Sbjct: 176 RSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of ClCG10G015230 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.0e-20
Identity = 54/114 (47.37%), Postives = 74/114 (64.91%), Query Frame = 0


Query: 91  LSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDR 150
           L RE+V  VM+ L L      + + E   + +   E++ +FEE EPS EE+KQ F+VFD 
Sbjct: 91  LCREDVGMVMKSLGL----STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDE 150

Query: 151 NRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNNDGKIDFNEFVKFME 205
           NRDGFID  +L +VL +LG  +G  + +C+RMI  FD + DG+IDF  FVKFME
Sbjct: 151 NRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of ClCG10G015230 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 42/91 (46.15%), Postives = 59/91 (64.84%), Query Frame = 0


Query: 114 DVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKG 173
           D+EEFG  +     T M E +E   E++++ FNVFD+NRDGFI  EEL  VL  LG  +G
Sbjct: 58  DIEEFGGLYQ----TIMEERDEE--EDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 117

Query: 174 IFIHDCKRMIARFDHNNDGKIDFNEFVKFME 205
             + DCKRMI++ D + DG ++F EF + M+
Sbjct: 118 RTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

[Close]

BLAST of ClCG10G015230 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 9.5e-14
Identity = 40/91 (43.96%), Postives = 60/91 (65.93%), Query Frame = 0


Query: 114 DVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKG 173
           D++EFGE +     T M EE+E   E++K+ FNVFD+N DGFI  +EL  VL  LG  +G
Sbjct: 58  DIDEFGELYK----TIMDEEDEEE-EDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQG 117

Query: 174 IFIHDCKRMIARFDHNNDGKIDFNEFVKFME 205
             + DCK+MI + D + DG++++ EF + M+
Sbjct: 118 KTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of ClCG10G015230 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.6e-13
Identity = 43/128 (33.59%), Postives = 78/128 (60.94%), Query Frame = 0


Query: 82  INTQQKN--CLLSREEVRFVMEKLEL---FCIPEEEDDVEEFGECFGGDEITAMFEENEP 141
           +N   KN   ++  +E+  +++K+++    C+     D+EEFGE +   +   + +E+E 
Sbjct: 26  LNESFKNLGIIIPEDELTQIIQKIDVNGDGCV-----DIEEFGELY---KTIMVEDEDEV 85

Query: 142 SFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNNDGKIDF 201
             E++K+ FNVFDRN DGFI  +EL  VL  LG  +G  + +C++MI + D + DG++++
Sbjct: 86  GEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNY 145

Query: 202 NEFVKFME 205
            EF + M+
Sbjct: 146 MEFRQMMK 145

[Close]

BLAST of ClCG10G015230 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.1e-62
Identity = 143/210 (68.10%), Postives = 161/210 (76.67%), Query Frame = 0


Query: 1   MFFRLEYYLITMQTRLSD-TNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIF 60
           MFFRLEYYLITM+TRL+D  +VQL P+P VFVDRISILPI F FA ++ +L++SLP  I 
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  QSPSKEKTSSPSNSTSQSDDQVINTQQKNC-LLSREEVRFVMEKLELFCIPEEEDDVEEF 120
           Q  SKEK  S  N +S+S DQ+I T Q+NC  +SREEVRFVMEKLELF   E +    + 
Sbjct: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120

Query: 121 GECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHD 180
           G     + I  MFEENEPS EELKQTFNVFDRNRDGFID  EL  VL LL SNKGIFIHD
Sbjct: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180

Query: 181 CKRMIARFDHNNDGKIDFNEFVKFMEVALS 209
           CK MIARFD NNDGKIDF+EFVKFMEVALS
Sbjct: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

[Close]

BLAST of ClCG10G015230 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 3.2e-57
Identity = 138/201 (68.66%), Postives = 157/201 (78.11%), Query Frame = 0


Query: 12  MQTRLSDT--NVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPL-SIFQSP-SKEKT 71
           MQT++++   +VQL P+PLVFVD IS LPI F FA ++H+LI+SLP  SI QS  SKEK 
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  SSPSNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDE- 131
            S SN +SQSDD++I T Q+NC +SREEVRFVMEKLELF   EE DD ++  E    DE 
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFW-REENDDDDDDEEIGASDEI 120

Query: 132 ITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARF 191
           I  MFEENEPS EELKQTFNVFDRN+DGFID  EL  VL LL SNKGIFIHDCK MIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 192 DHNNDGKIDFNEFVKFMEVAL 208
           D NNDGKIDF+EFVKFMEVAL
Sbjct: 181 DLNNDGKIDFHEFVKFMEVAL 200

[Close]

BLAST of ClCG10G015230 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 1.1e-52
Identity = 123/195 (63.08%), Postives = 146/195 (74.87%), Query Frame = 0


Query: 20  NVQLVPT------PLVFVDRISILPIFFIFAVKLHDLIKSLPLSIFQSPSKEKTSSPSNS 79
           N+QL+P+      PLVF+DR S      IFA  +HDL+ SL  S FQSP K+K ++P + 
Sbjct: 9   NLQLIPSTTTTTPPLVFIDRAS------IFAGTIHDLV-SLAFSFFQSPPKDK-AAPQSR 68

Query: 80  TSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAMFEE 139
            SQS+       +++ L+SREEV+ VMEKLEL+C  EE+D+++   ECFG DE+  MFEE
Sbjct: 69  ASQSE------SKEDWLMSREEVKSVMEKLELYCNEEEDDEID---ECFGRDEVRGMFEE 128

Query: 140 NEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNNDGK 199
           NEPS EELKQTFNVFDRNRDGFIDA+ELH VLGLL SN  IFIHDCK MIARFD NND K
Sbjct: 129 NEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQK 186

Query: 200 IDFNEFVKFMEVALS 209
           IDFNEFVKFMEVALS
Sbjct: 189 IDFNEFVKFMEVALS 186

[Close]

BLAST of ClCG10G015230 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 2.0e-51
Identity = 120/198 (60.61%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 11  TMQTRLSDTNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIFQSPSKEKTSSP 70
           T+  +L  +   ++P PLVF+DR S      IFA  +HDL+ SL  S FQSP KEK ++P
Sbjct: 7   TINLQLIPSTTTMMPPPLVFIDRAS------IFADTIHDLV-SLAFSFFQSPPKEK-AAP 66

Query: 71  SNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAM 130
            +  SQS+       +++ L+SREEV+ VMEKLEL+C  E +D+++   ECFG +E+  M
Sbjct: 67  QSRASQSEG------KEDWLMSREEVKSVMEKLELYCNEEGDDEID---ECFGREEVRGM 126

Query: 131 FEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNN 190
           FEENEPS EELKQTFNVFDRNRDGFIDA ELH VLGLL SN  IFIHDCK MIARFD NN
Sbjct: 127 FEENEPSLEELKQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNN 186

Query: 191 DGKIDFNEFVKFMEVALS 209
           D KIDFNEFVKFMEV+LS
Sbjct: 187 DEKIDFNEFVKFMEVSLS 187

[Close]

BLAST of ClCG10G015230 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 1.1e-49
Identity = 120/197 (60.91%), Postives = 140/197 (71.07%), Query Frame = 0


Query: 12  MQTRLSDTNVQLVPTPLVFVDRISILPIFFIFAVKLHDLIKSLPLSIFQSPSKEKTSSPS 71
           M ++    N++L PT L+ VDRISI  I F F  ++HDLI S P S+F SP KEK S   
Sbjct: 1   MPSQFPIVNLKLAPT-LLLVDRISISSILFSFVDRIHDLI-SQPWSLFHSPPKEKVSPQG 60

Query: 72  NSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAMF 131
           NS          TQ KN  +S+EEV+FVMEKLE FC  EE   +EE     GG+EI A+F
Sbjct: 61  NSLESE-----ATQHKNSSISKEEVKFVMEKLEFFCSDEE---IEE--SWLGGEEIGAIF 120

Query: 132 EENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNND 191
            E+EPS EELKQTFNVFD+NRDG IDA+EL  VLGLL  N+G+FIHDC RMIARFDHN D
Sbjct: 121 YESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKD 180

Query: 192 GKIDFNEFVKFMEVALS 209
           GKIDFNEFVKFME+ALS
Sbjct: 181 GKIDFNEFVKFMEIALS 185

[Close]

BLAST of ClCG10G015230 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 108.6 bits (270), Expect = 6.1e-24
Identity = 62/143 (43.36%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 64  KEKTSSPSNSTSQSDDQVINTQQKNCLLSREEVRFVMEKLELFCIPEEEDDVEEFGECFG 123
           +++T +        DD   +    +  +SREE   VM  L LF      DD  +  E + 
Sbjct: 56  QQETLTKQEDDDDDDDDDDDDDDDDIDISREEAEMVMRSLGLF----YNDD--QLQEQYS 115

Query: 124 GDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMI 183
             E++++FEE E S EE+KQ F+VFD N+DGFIDA EL +VL +LG  +G ++ +C  MI
Sbjct: 116 AKEVSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMI 175

Query: 184 ARFDHNNDGKIDFNEFVKFMEVA 207
              D N DGKIDFNEFVKFME +
Sbjct: 176 RSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of ClCG10G015230 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 101.7 bits (252), Expect = 7.4e-22
Identity = 54/114 (47.37%), Postives = 74/114 (64.91%), Query Frame = 0


Query: 91  LSREEVRFVMEKLELFCIPEEEDDVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDR 150
           L RE+V  VM+ L L      + + E   + +   E++ +FEE EPS EE+KQ F+VFD 
Sbjct: 91  LCREDVGMVMKSLGL----STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDE 150

Query: 151 NRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNNDGKIDFNEFVKFME 205
           NRDGFID  +L +VL +LG  +G  + +C+RMI  FD + DG+IDF  FVKFME
Sbjct: 151 NRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of ClCG10G015230 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-15
Identity = 42/91 (46.15%), Postives = 59/91 (64.84%), Query Frame = 0


Query: 114 DVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKG 173
           D+EEFG  +     T M E +E   E++++ FNVFD+NRDGFI  EEL  VL  LG  +G
Sbjct: 58  DIEEFGGLYQ----TIMEERDEE--EDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 117

Query: 174 IFIHDCKRMIARFDHNNDGKIDFNEFVKFME 205
             + DCKRMI++ D + DG ++F EF + M+
Sbjct: 118 RTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

[Close]

BLAST of ClCG10G015230 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 78.6 bits (192), Expect = 6.7e-15
Identity = 40/91 (43.96%), Postives = 60/91 (65.93%), Query Frame = 0


Query: 114 DVEEFGECFGGDEITAMFEENEPSFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKG 173
           D++EFGE +     T M EE+E   E++K+ FNVFD+N DGFI  +EL  VL  LG  +G
Sbjct: 58  DIDEFGELYK----TIMDEEDEEE-EDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQG 117

Query: 174 IFIHDCKRMIARFDHNNDGKIDFNEFVKFME 205
             + DCK+MI + D + DG++++ EF + M+
Sbjct: 118 KTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of ClCG10G015230 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 76.6 bits (187), Expect = 2.6e-14
Identity = 43/128 (33.59%), Postives = 78/128 (60.94%), Query Frame = 0


Query: 82  INTQQKN--CLLSREEVRFVMEKLEL---FCIPEEEDDVEEFGECFGGDEITAMFEENEP 141
           +N   KN   ++  +E+  +++K+++    C+     D+EEFGE +   +   + +E+E 
Sbjct: 26  LNESFKNLGIIIPEDELTQIIQKIDVNGDGCV-----DIEEFGELY---KTIMVEDEDEV 85

Query: 142 SFEELKQTFNVFDRNRDGFIDAEELHKVLGLLGSNKGIFIHDCKRMIARFDHNNDGKIDF 201
             E++K+ FNVFDRN DGFI  +EL  VL  LG  +G  + +C++MI + D + DG++++
Sbjct: 86  GEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNY 145

Query: 202 NEFVKFME 205
            EF + M+
Sbjct: 146 MEFRQMMK 145

The following BLAST results are available for this feature:

BLAST of ClCG10G015230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904514.1	3.1e-78	81.73	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
KGN59556.1	2.4e-62	68.10	hypothetical protein Csa_001548 [Cucumis sativus]	[more]
XP_008443616.1	6.7e-57	68.66	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
XP_023528818.1	6.7e-57	63.08	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
XP_004147503.1	3.3e-56	66.33	probable calcium-binding protein CML45 [Cucumis sativus]	[more]

BLAST of ClCG10G015230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	8.5e-23	43.36	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	1.0e-20	47.37	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SRR7	1.9e-14	46.15	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	9.5e-14	43.96	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9ZR02	3.6e-13	33.59	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of ClCG10G015230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ5	1.1e-62	68.10	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
A0A1S3B8I1	3.2e-57	68.66	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
A0A6J1F854	1.1e-52	63.08	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1J5Q6	2.0e-51	60.61	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1KQS0	1.1e-49	60.91	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]

BLAST of ClCG10G015230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	6.1e-24	43.36	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	7.4e-22	47.37	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	1.4e-15	46.15	ARF-GAP domain 11	[more]
AT1G05990.1	6.7e-15	43.96	EF hand calcium-binding protein family	[more]
AT4G03290.1	2.6e-14	33.59	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 178..206 e-value: 7.2E-4 score: 28.8 coord: 140..168 e-value: 1.9E-6 score: 37.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 138..204 e-value: 6.9E-12 score: 45.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 174..208 score: 11.528597
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 136..171 score: 15.517639
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 140..203 e-value: 2.47839E-15 score: 65.6469
None	No IPR available	GENE3D	1.10.238.10		coord: 123..206 e-value: 8.6E-21 score: 76.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..81
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 63..206
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 63..206
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 187..199
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 149..161
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 87..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG10G015230.2	ClCG10G015230.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding