ClCG10G015120 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG10G015120
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	ribonuclease MC-like
Location	CG_Chr10: 29631625 .. 29633022 (-)
RNA-Seq Expression	ClCG10G015120
Synteny	ClCG10G015120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGAAAGAAATTGTGATGATTTTGAGTTGTATGTTGGTGATGATAGTAACTAGTTGTGAAGGGGTCAATCAGTTTGACTACATGCAAGTTGTGATCCAATGGCAACCCGCCACGTGTAGTGATTGGTACAATCCTAATTGTTTCCGCGCCCCAGATGATAGGTTTAGTATCCATGGCGTGTGGCCCAGCCTATACAACGGCGGCCAACCCGTTTGTTCCGGTCGTCCCTTCGATGTAAATCAGGTAAATTTTACATATTTTTTGAACTAACAATATTCCCAATTATTATTCGAACTGTCGCTAATCAAAAGTTAGAACTATTATTTTATTATTTTGTTAACATATATATACACATGACTCAAATGATATTTAACATACAACCATTCTATCCAAAAAGAAAGAAAAAAAATCTAATTTGAGAATGACAAACCTAAAGAGACATTATCTTAATTTTTATAAGATACCTAAATTATTATTATTATTATTTTTTTTTTTTTCATTTTCGATGGAACACTATTTTTTCTTTATCTTGTACGTGAAGAGTCTATCTAAAAACTTTTAAGATATAGGAAGGTAAAAGTATTTTTAGAAAAGGGTTAGTTCATTAATTCCAGCAGCATCCACAACCCGAAAAGAAATAAGTCATTTTCATCACTTCATTAATAGACCAGTATGTGAGAAACATACGACATATATAGTGACAAAAAAACAGATAAGCTCAAAGAGAGGCACTACAATTTGTAAAATGCTTTGATTTGTATAACCAAAACACCAACGAGATTTCTAATAACTTCCCAAATCTGCATGTCGTTGCTATTGTCGGTGTTTTTGAAAGTGCAATTATTTCTTTCATTACAAAGAAACCGAGACCAACAGATGGCGATTGAGATATTTTGAGATATATATATACATAATTACTAAAAGTTGATTTTATTGATTGTCCAGATTAGCATACTCCAACCCGGAATCAGTGAAATATGGCCAAATATTGCCACAGGAGATAACTTATGGCTATGGAGACACGAGTGGGATGTACATGGAACTTGTACCGAGGCATTTTTATTTGACCAAACACGATATTTCAGCTTTGCAATGGACACATATATTGAAAACAATCTGTTGTTTATTCTTGGGAATAGCCAAATTACACCAAATGGACGACTTTATTCAAGGGATCAAGTTTATACGGCAATAAAAGCAAAGACCGGAAAAAAGCCAGCCGTTAGATGCAACTACAACTATCGGGATGGATCAGTTCAACAATTGCATGAAATAACTCTTTGTTACAATTGGAATGCTACGGCTTTTGTGGACTGCCCTTTGAATGCCAACAAATGCGATAATAATTTTGTGTGGTATCCATGGTTCACTACCAAGGCTTTATCTATCTAA

mRNA sequence

ATGGAGAAGAAAGAAATTGTGATGATTTTGAGTTGTATGTTGGTGATGATAGTAACTAGTTGTGAAGGGGTCAATCAGTTTGACTACATGCAAGTTGTGATCCAATGGCAACCCGCCACGTGTAGTGATTGGTACAATCCTAATTGTTTCCGCGCCCCAGATGATAGGTTTAGTATCCATGGCGTGTGGCCCAGCCTATACAACGGCGGCCAACCCGTTTGTTCCGGTCGTCCCTTCGATGTAAATCAGATTAGCATACTCCAACCCGGAATCAGTGAAATATGGCCAAATATTGCCACAGGAGATAACTTATGGCTATGGAGACACGAGTGGGATGTACATGGAACTTGTACCGAGGCATTTTTATTTGACCAAACACGATATTTCAGCTTTGCAATGGACACATATATTGAAAACAATCTGTTGTTTATTCTTGGGAATAGCCAAATTACACCAAATGGACGACTTTATTCAAGGGATCAAGTTTATACGGCAATAAAAGCAAAGACCGGAAAAAAGCCAGCCGTTAGATGCAACTACAACTATCGGGATGGATCAGTTCAACAATTGCATGAAATAACTCTTTGTTACAATTGGAATGCTACGGCTTTTGTGGACTGCCCTTTGAATGCCAACAAATGCGATAATAATTTTGTGTGGTATCCATGGTTCACTACCAAGGCTTTATCTATCTAA

Coding sequence (CDS)

ATGGAGAAGAAAGAAATTGTGATGATTTTGAGTTGTATGTTGGTGATGATAGTAACTAGTTGTGAAGGGGTCAATCAGTTTGACTACATGCAAGTTGTGATCCAATGGCAACCCGCCACGTGTAGTGATTGGTACAATCCTAATTGTTTCCGCGCCCCAGATGATAGGTTTAGTATCCATGGCGTGTGGCCCAGCCTATACAACGGCGGCCAACCCGTTTGTTCCGGTCGTCCCTTCGATGTAAATCAGATTAGCATACTCCAACCCGGAATCAGTGAAATATGGCCAAATATTGCCACAGGAGATAACTTATGGCTATGGAGACACGAGTGGGATGTACATGGAACTTGTACCGAGGCATTTTTATTTGACCAAACACGATATTTCAGCTTTGCAATGGACACATATATTGAAAACAATCTGTTGTTTATTCTTGGGAATAGCCAAATTACACCAAATGGACGACTTTATTCAAGGGATCAAGTTTATACGGCAATAAAAGCAAAGACCGGAAAAAAGCCAGCCGTTAGATGCAACTACAACTATCGGGATGGATCAGTTCAACAATTGCATGAAATAACTCTTTGTTACAATTGGAATGCTACGGCTTTTGTGGACTGCCCTTTGAATGCCAACAAATGCGATAATAATTTTGTGTGGTATCCATGGTTCACTACCAAGGCTTTATCTATCTAA

Protein sequence

MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPNCFRAPDDRFSIHGVWPSLYNGGQPVCSGRPFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCTEAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWFTTKALSI

Homology

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BLAST of ClCG10G015120 vs. NCBI nr
Match: XP_038903744.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 391.3 bits (1004), Expect = 5.6e-105
Identity = 180/233 (77.25%), Postives = 196/233 (84.12%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYN--PNCFRAPDDRFS 60
           MEKKEI +ILS MLVMIV+ CEGVN +DY+QVVIQWQPATC+DW    P CF+ PD+RFS
Sbjct: 1   MEKKEIGLILS-MLVMIVSCCEGVNDYDYLQVVIQWQPATCTDWKKPYPYCFQDPDNRFS 60

Query: 61  IHGVWPSLYNGGQPVCSGRPFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 120
           IHG+WPSLY+G Q  C G PFDVNQISILQPGISEIWPN+ TG N WLW HEWD HGTCT
Sbjct: 61  IHGMWPSLYSGAQTHCFGSPFDVNQISILQPGISEIWPNVVTGKNQWLWGHEWDKHGTCT 120

Query: 121 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 180
           E+ LFDQ  YFSF MD YIENNL FILG SQITPNG+LYSR Q+Y AI AKTGK PAVRC
Sbjct: 121 ESILFDQEEYFSFTMDKYIENNLSFILGKSQITPNGQLYSRAQIYAAISAKTGKTPAVRC 180

Query: 181 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWFTTKALSI 232
           NYNY  G V+QLHEITLCYNWNATAF DCPLNANKCDN F+WYPW TTKALS+
Sbjct: 181 NYNYWSG-VEQLHEITLCYNWNATAFEDCPLNANKCDNTFMWYPWSTTKALSV 231

[Close]

BLAST of ClCG10G015120 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 354.4 bits (908), Expect = 7.6e-94
Identity = 161/232 (69.40%), Postives = 191/232 (82.33%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPN--CFRAPDDRFS 60
           M K+EIV++LS  +++++ SCEG+N+FDY+QVV+QWQPATCS    P   C++ PD+R+S
Sbjct: 1   MAKREIVLVLS--MLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYS 60

Query: 61  IHGVWPSLYNGGQPVCSGRPFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 120
           IHGVWPSLY+G    CSG PF+ N ISILQPGISEIWPNI  G+NLWLW HEWD HGTCT
Sbjct: 61  IHGVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCT 120

Query: 121 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 180
           E  LFDQ RYFSFAMDTYI+ NLL +LGNSQITPNG++YSRDQVY AI+AKTGK PAVRC
Sbjct: 121 EEILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWFTTKALS 231
           NYN   G  QQ+HE++LCYN +A+A VDCPLNANKCDN FVWY W T KA+S
Sbjct: 181 NYNRWTGE-QQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVS 229

[Close]

BLAST of ClCG10G015120 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 341.3 bits (874), Expect = 6.6e-90
Identity = 154/230 (66.96%), Postives = 183/230 (79.57%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPNCFRAPDDRFSIH 60
           M ++EI++++  MLV +V  CEG+N+FDY+QVVIQWQPATCSD+  P CF+AP +RFSIH
Sbjct: 1   MAQREIIVLVLSMLV-LVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIH 60

Query: 61  GVWPSLYNGGQPVCSGRPFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCTEA 120
           GVWPSLY+G    CSG PFD NQIS LQPGISEIWPN+ATGDN WLW+HEWD HGTC+E+
Sbjct: 61  GVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSES 120

Query: 121 FLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNY 180
             +DQ  YF F MD YI NNL  IL N+QITPNGR Y+RDQVY AI+AKTGK PAVRCNY
Sbjct: 121 TFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNY 180

Query: 181 NYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWFTTKALS 231
           N R G+  QLHEI+LC+  +A+ F DCPLNANKC  +FVW  W T KA+S
Sbjct: 181 NSRTGA-SQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 228

[Close]

BLAST of ClCG10G015120 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 222.6 bits (566), Expect = 3.4e-54
Identity = 107/225 (47.56%), Postives = 144/225 (64.00%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSD-WYNPNCFRAPDDRFSI 60
           M K+EI+++LS  L   ++S EGVN+FDY QVV+QWQPATCS       C + P+DR+S+
Sbjct: 1   MGKREILLVLSMFLA--ISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSV 60

Query: 61  HGVWPSLYNGGQPVCSGR-PFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 120
           HGVWPSL +G  P CSG  P+D   I  +Q  + + WPN+ TG N   W +EW  HGTC+
Sbjct: 61  HGVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS 120

Query: 121 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 180
            A+L +Q  YF+F +  Y   +L  IL    I PNG+ Y++  V  AI + TGK PA+RC
Sbjct: 121 SAYL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRC 180

Query: 181 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPW 224
           N NYR G++ QLHEI+LC++ N    +DCP N   C+  FVW  W
Sbjct: 181 NNNYRTGNM-QLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSW 221

[Close]

BLAST of ClCG10G015120 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-48
Identity = 105/225 (46.67%), Postives = 143/225 (63.56%), Query Frame = 0


Query: 2   EKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATC-SDWYNPNCFRAPDDRFSIH 61
           ++ EIV+ILS  +++++   EGVN+FDY QVV+QWQPATC S      C + PD+RFSIH
Sbjct: 3   KRSEIVLILS--MLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIH 62

Query: 62  GVWPSLYNGGQPVCSGR-PFDVNQI-SILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 121
           GVWPSL + G  VCSG  P+D  Q+ S ++  + + WP++    +   W HEW +HGTC+
Sbjct: 63  GVWPSLLS-GDAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCS 122

Query: 122 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 181
            A+L DQ  YF F +  Y   ++L IL    + PNG LY+R  V   I + TGK PA+RC
Sbjct: 123 YAYL-DQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRC 182

Query: 182 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPW 224
           N N+  G V QLHEI+LC+     +  DCP NA KC++ FVW  W
Sbjct: 183 NNNFWSGDV-QLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSW 222

[Close]

BLAST of ClCG10G015120 vs. ExPASy Swiss-Prot
Match: O80324 (Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 5.2e-29
Identity = 74/227 (32.60%), Postives = 116/227 (51.10%), Query Frame = 0


Query: 6   IVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPNCFRAPDDRFSIHGVWPS 65
           I M+     ++++ SC     ++Y Q   Q+QPA C+    P C   PD  F++HG+WPS
Sbjct: 7   IYMVPMVFSLIVLISCSSTMGYNYFQFTQQYQPAVCNSNPTP-CKDPPDKLFTVHGLWPS 66

Query: 66  LYNGGQPV-CSGRPFDVNQISILQPGISEIWPNIA-TGDNLWLWRHEWDVHGTCTEA-FL 125
              G  P+ C  +     QI  L   +  IWPN+    D++  W  +W+ HG+C +A  +
Sbjct: 67  NDVGDDPIYCKNKTIKSQQIGNLTAQLIIIWPNVLDRTDHVGFWNRQWNKHGSCGKAPTI 126

Query: 126 FDQTRYFSFAMDTYI--ENNLLFILGNSQITPNGRLYSRDQVYTAIKAKT-GKKPAVRCN 185
            D+  YF   +  YI  + N+  IL  ++I P G++  RD +  AI+  T  KKP ++C 
Sbjct: 127 KDEMHYFKTVIKMYITQKQNVSEILSRAKIEPEGKIRRRDDIINAIRLGTKDKKPKLKCQ 186

Query: 186 YNYRDGSVQQLHEITLCYNWNATAFVDCPLNANK-----CDNNFVWY 222
            N       +L EIT+C + N T F+DCP ++ K     C  N + Y
Sbjct: 187 KN---NQTTELVEITICSDRNLTQFIDCPRSSFKGSPFHCPTNHILY 229

[Close]

BLAST of ClCG10G015120 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.8e-29
Identity = 67/195 (34.36%), Postives = 100/195 (51.28%), Query Frame = 0


Query: 27  FDYMQVVIQWQPATCSDWYNPNCFRAPDDRFSIHGVWPSLYNGGQPVC-SGRPFDVNQIS 86
           FD    V QW PA CS   + +C  +    F+IHG+WP         C  G PFD+ +IS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 87  ILQPGISEIWPNIATGDNLWLWRHEWDVHGTCTEAFLFDQTRYFSFAMDTYIENNLLFIL 146
            LQ  ++ +WPN+   +N   W HEW  HGTC+E+  F+Q  YF  A+D     +++  L
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSES-TFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 147 GNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNYRDGSVQQLHEITLCYNWNATAFV 206
                 PNGR  SR  +   +KAK GK P +RC  + +   V  L ++  C+  + +  +
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQT-KVSYLVQVVACFAQDGSTLI 180

Query: 207 DCPLNANKCDNNFVW 221
           DC    + C  NF++
Sbjct: 181 DC--TRDTCGANFIF 191

[Close]

BLAST of ClCG10G015120 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 2.2e-27
Identity = 72/224 (32.14%), Postives = 116/224 (51.79%), Query Frame = 0


Query: 13  MLVMIVTSCEGV----NQFDYMQVVIQWQPATCSDWYNPNCFRA---PDDRFSIHGVWPS 72
           ++++I+T C  V      FD+   V QW P +  D     C+     P   F IHG+WP+
Sbjct: 11  LILLIITQCLSVLNAAKDFDFFYFVQQW-PGSYCDTKQSCCYPTTGKPAADFGIHGLWPN 70

Query: 73  LYNGGQPV-CS-GRPFDVNQISILQPGISEIWPNIA--TGDNLWLWRHEWDVHGTCTEAF 132
             +G  P  C    P+D +QIS L   + + WP +A  +G     W HEW+ HGTC E+ 
Sbjct: 71  NNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESV 130

Query: 133 LFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYN 192
           L +Q  YF  A+D   + +LL IL  + I P+G  Y    +  AIK+  G  P ++CN +
Sbjct: 131 LTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVD 190

Query: 193 YRDGSVQQLHEITLCYNWNATAFVDCPL-NANKCDNNFVWYPWF 225
               S  QL+++ +C + + ++ ++CP+    KC  + + +P F
Sbjct: 191 QSGNS--QLYQVYICVDGSGSSLIECPIFPGGKCGTS-IEFPTF 230

[Close]

BLAST of ClCG10G015120 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.8e-26
Identity = 69/208 (33.17%), Postives = 108/208 (51.92%), Query Frame = 0


Query: 10  LSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPNCF---RAPDDRFSIHGVWPSL 69
           L  +L  + ++      FD+   V QW P +  D     C+     P   F IHG+WP+ 
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLWPNY 72

Query: 70  YNGGQP--VCSGRPFDVNQISILQPGISEIWPNIA--TGDNLWLWRHEWDVHGTCTEAFL 129
            +G  P    + +PFD + IS L   + + WP +A  +G     W HEW+ HGTC+E+ +
Sbjct: 73  KDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES-V 132

Query: 130 FDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNY 189
            DQ  YF  A++   + NLL  L  + I P+G+ YS + +  +IK   G  P V CN   
Sbjct: 133 IDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN--- 192

Query: 190 RDGS-VQQLHEITLCYNWNATAFVDCPL 210
           RDGS   QL+++ LC + + +  ++CP+
Sbjct: 193 RDGSGNSQLYQVYLCVDRSGSGLIECPV 215

[Close]

BLAST of ClCG10G015120 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 70/206 (33.98%), Postives = 106/206 (51.46%), Query Frame = 0


Query: 27  FDYMQVVIQWQPATCSDWYNPNCF---RAPDDRFSIHGVWPSLYNGGQPV-CS-GRPFDV 86
           FD+   V+QW  A C D  +  C+     P   F IHG+WP+   GG P  C+    FD 
Sbjct: 22  FDFFYFVLQWPGAYC-DSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 81

Query: 87  NQISILQPGISEIWPNIA--TGDNLWLWRHEWDVHGTCTEAFLFDQTRYFSFAMDTYIEN 146
            ++S L   +   WP ++  + D +  W HEW+ HGTC E+ L DQ  YF   +    + 
Sbjct: 82  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKA 141

Query: 147 NLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNYRDGSVQQLHEITLCYNW 206
           NLL  L N+ I P+ + Y    +   IK   G  P + CN +    S  QL++I LC + 
Sbjct: 142 NLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNS--QLYQIYLCVDT 201

Query: 207 NATAFVDCPLNAN-KCDNNFVWYPWF 225
           +A+ F++CP+  + +CD+  V +P F
Sbjct: 202 SASKFINCPVMPHGRCDSR-VQFPKF 222

[Close]

BLAST of ClCG10G015120 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.7e-94
Identity = 161/232 (69.40%), Postives = 191/232 (82.33%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPN--CFRAPDDRFS 60
           M K+EIV++LS  +++++ SCEG+N+FDY+QVV+QWQPATCS    P   C++ PD+R+S
Sbjct: 1   MAKREIVLVLS--MLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYS 60

Query: 61  IHGVWPSLYNGGQPVCSGRPFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 120
           IHGVWPSLY+G    CSG PF+ N ISILQPGISEIWPNI  G+NLWLW HEWD HGTCT
Sbjct: 61  IHGVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCT 120

Query: 121 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 180
           E  LFDQ RYFSFAMDTYI+ NLL +LGNSQITPNG++YSRDQVY AI+AKTGK PAVRC
Sbjct: 121 EEILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWFTTKALS 231
           NYN   G  QQ+HE++LCYN +A+A VDCPLNANKCDN FVWY W T KA+S
Sbjct: 181 NYNRWTGE-QQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVS 229

[Close]

BLAST of ClCG10G015120 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.2e-90
Identity = 154/230 (66.96%), Postives = 183/230 (79.57%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPNCFRAPDDRFSIH 60
           M ++EI++++  MLV +V  CEG+N+FDY+QVVIQWQPATCSD+  P CF+AP +RFSIH
Sbjct: 1   MAQREIIVLVLSMLV-LVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIH 60

Query: 61  GVWPSLYNGGQPVCSGRPFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCTEA 120
           GVWPSLY+G    CSG PFD NQIS LQPGISEIWPN+ATGDN WLW+HEWD HGTC+E+
Sbjct: 61  GVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSES 120

Query: 121 FLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNY 180
             +DQ  YF F MD YI NNL  IL N+QITPNGR Y+RDQVY AI+AKTGK PAVRCNY
Sbjct: 121 TFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNY 180

Query: 181 NYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWFTTKALS 231
           N R G+  QLHEI+LC+  +A+ F DCPLNANKC  +FVW  W T KA+S
Sbjct: 181 NSRTGA-SQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 228

[Close]

BLAST of ClCG10G015120 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.7e-54
Identity = 107/225 (47.56%), Postives = 144/225 (64.00%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSD-WYNPNCFRAPDDRFSI 60
           M K+EI+++LS  L   ++S EGVN+FDY QVV+QWQPATCS       C + P+DR+S+
Sbjct: 1   MGKREILLVLSMFLA--ISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSV 60

Query: 61  HGVWPSLYNGGQPVCSGR-PFDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 120
           HGVWPSL +G  P CSG  P+D   I  +Q  + + WPN+ TG N   W +EW  HGTC+
Sbjct: 61  HGVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS 120

Query: 121 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 180
            A+L +Q  YF+F +  Y   +L  IL    I PNG+ Y++  V  AI + TGK PA+RC
Sbjct: 121 SAYL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRC 180

Query: 181 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPW 224
           N NYR G++ QLHEI+LC++ N    +DCP N   C+  FVW  W
Sbjct: 181 NNNYRTGNM-QLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSW 221

[Close]

BLAST of ClCG10G015120 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 8.0e-49
Identity = 105/225 (46.67%), Postives = 143/225 (63.56%), Query Frame = 0


Query: 2   EKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATC-SDWYNPNCFRAPDDRFSIH 61
           ++ EIV+ILS  +++++   EGVN+FDY QVV+QWQPATC S      C + PD+RFSIH
Sbjct: 3   KRSEIVLILS--MLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIH 62

Query: 62  GVWPSLYNGGQPVCSGR-PFDVNQI-SILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 121
           GVWPSL + G  VCSG  P+D  Q+ S ++  + + WP++    +   W HEW +HGTC+
Sbjct: 63  GVWPSLLS-GDAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCS 122

Query: 122 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 181
            A+L DQ  YF F +  Y   ++L IL    + PNG LY+R  V   I + TGK PA+RC
Sbjct: 123 YAYL-DQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRC 182

Query: 182 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPW 224
           N N+  G V QLHEI+LC+     +  DCP NA KC++ FVW  W
Sbjct: 183 NNNFWSGDV-QLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSW 222

[Close]

BLAST of ClCG10G015120 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.4e-45
Identity = 96/226 (42.48%), Postives = 133/226 (58.85%), Query Frame = 0


Query: 1   MEKKEIVMILSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDW-YNPNCFRAPDDRFSI 60
           MEKK IV++ S ++V  + SC+    ++Y QV +QWQPATCSD      C   P DRFSI
Sbjct: 1   MEKKGIVLVFSTLVV--IASCD----YNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSI 60

Query: 61  HGVWPSLYNGGQPVCSGRP-FDVNQISILQPGISEIWPNIATGDNLWLWRHEWDVHGTCT 120
           HGVWP+L +G  P C G P +D ++IS ++  ++  WP++  G N  LW HEW  HGTC+
Sbjct: 61  HGVWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCS 120

Query: 121 EAFLFDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRC 180
              L  Q  YF+F +DTY + ++   L    I P+  +YS+  +  AI+  T K P +RC
Sbjct: 121 YPSL-SQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRC 180

Query: 181 NYNYRDGSVQQLHEITLCYNWNATAFVDCPLNANKCDNNFVWYPWF 225
           N N      +QLHEI LC+  +  A +DCP N   C N   WYPW+
Sbjct: 181 NTNDYSRE-RQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPWY 218

[Close]

BLAST of ClCG10G015120 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 124.8 bits (312), Expect = 9.1e-29
Identity = 70/191 (36.65%), Postives = 103/191 (53.93%), Query Frame = 0


Query: 27  FDYMQVVIQWQPATCSDWYNPNCFRA---PDDRFSIHGVWPSLYNGGQPVCSGRP---FD 86
           FD+   V+QW  A C D     C+     P   F IHG+WP+ YNGG    +  P   FD
Sbjct: 28  FDFFYFVLQWPGAYC-DTSRACCYPTSGKPAADFGIHGLWPN-YNGGSWPSNCDPDSQFD 87

Query: 87  VNQISILQPGISEIWPNIA--TGDNLWLWRHEWDVHGTCTEAFLFDQTRYFSFAMDTYIE 146
            +QIS L   + + WP ++  + +    W HEW+ HGTC+E+ + DQ  YF  A+    +
Sbjct: 88  RSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSES-VMDQHEYFENALKLKQK 147

Query: 147 NNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNYRDGSVQQLHEITLCYN 206
            NLL IL NS I P+   Y+ D++  AIK   G  P + CN +    +  QLH+I +C +
Sbjct: 148 ANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNA--QLHQIYICVD 207

Query: 207 WNATAFVDCPL 210
            + T F++CP+
Sbjct: 208 TSGTEFIECPV 213

[Close]

BLAST of ClCG10G015120 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 69/208 (33.17%), Postives = 108/208 (51.92%), Query Frame = 0


Query: 10  LSCMLVMIVTSCEGVNQFDYMQVVIQWQPATCSDWYNPNCF---RAPDDRFSIHGVWPSL 69
           L  +L  + ++      FD+   V QW P +  D     C+     P   F IHG+WP+ 
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLWPNY 72

Query: 70  YNGGQP--VCSGRPFDVNQISILQPGISEIWPNIA--TGDNLWLWRHEWDVHGTCTEAFL 129
            +G  P    + +PFD + IS L   + + WP +A  +G     W HEW+ HGTC+E+ +
Sbjct: 73  KDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES-V 132

Query: 130 FDQTRYFSFAMDTYIENNLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNY 189
            DQ  YF  A++   + NLL  L  + I P+G+ YS + +  +IK   G  P V CN   
Sbjct: 133 IDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN--- 192

Query: 190 RDGS-VQQLHEITLCYNWNATAFVDCPL 210
           RDGS   QL+++ LC + + +  ++CP+
Sbjct: 193 RDGSGNSQLYQVYLCVDRSGSGLIECPV 215

[Close]

BLAST of ClCG10G015120 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 118.2 bits (295), Expect = 8.5e-27
Identity = 70/206 (33.98%), Postives = 106/206 (51.46%), Query Frame = 0


Query: 27  FDYMQVVIQWQPATCSDWYNPNCF---RAPDDRFSIHGVWPSLYNGGQPV-CS-GRPFDV 86
           FD+   V+QW  A C D  +  C+     P   F IHG+WP+   GG P  C+    FD 
Sbjct: 22  FDFFYFVLQWPGAYC-DSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 81

Query: 87  NQISILQPGISEIWPNIA--TGDNLWLWRHEWDVHGTCTEAFLFDQTRYFSFAMDTYIEN 146
            ++S L   +   WP ++  + D +  W HEW+ HGTC E+ L DQ  YF   +    + 
Sbjct: 82  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKA 141

Query: 147 NLLFILGNSQITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNYRDGSVQQLHEITLCYNW 206
           NLL  L N+ I P+ + Y    +   IK   G  P + CN +    S  QL++I LC + 
Sbjct: 142 NLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNS--QLYQIYLCVDT 201

Query: 207 NATAFVDCPLNAN-KCDNNFVWYPWF 225
           +A+ F++CP+  + +CD+  V +P F
Sbjct: 202 SASKFINCPVMPHGRCDSR-VQFPKF 222

[Close]

BLAST of ClCG10G015120 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-18
Identity = 54/187 (28.88%), Postives = 85/187 (45.45%), Query Frame = 0


Query: 26  QFDYMQVVIQWQPATCSDWYN----PNCFRAPD--DRFSIHGVWPSLYNGGQPVCSGR-P 85
           +FDY  + +QW    C    +      C R  D   +F+IHG+WP   +G  P C  R  
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSD 90

Query: 86  FDVNQISILQPGISEIWPNIA-------TGDNLWLWRHEWDVHGTCTEAFLFDQTRYFSF 145
           F   +IS L  G+ + WP+++        G     W HEW+ HGTC+     D+  YF  
Sbjct: 91  FKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLT 150

Query: 146 AMDTYIENNLLFILGNS-QITPNGRLYSRDQVYTAIKAKTGKKPAVRCNYNYRDGSVQQL 198
            ++ Y+++N+  +L  +  +  N   Y    + TAI+      P V C    RD     +
Sbjct: 151 TLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCK---RDA----I 210

The following BLAST results are available for this feature:

BLAST of ClCG10G015120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903744.1	5.6e-105	77.25	ribonuclease MC-like [Benincasa hispida]	[more]
AEM42996.1	7.6e-94	69.40	S-RNase [Siraitia grosvenorii]	[more]
XP_022149793.1	6.6e-90	66.96	ribonuclease MC-like [Momordica charantia]	[more]
XP_022158939.1	3.4e-54	47.56	ribonuclease MC-like [Momordica charantia]	[more]
XP_022156540.1	1.7e-48	46.67	ribonuclease MC-like [Momordica charantia]	[more]

BLAST of ClCG10G015120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80324	5.2e-29	32.60	Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
P23540	6.8e-29	34.36	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80022	2.2e-27	32.14	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42813	1.8e-26	33.17	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	1.2e-25	33.98	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]

BLAST of ClCG10G015120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7NBR4	3.7e-94	69.40	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	3.2e-90	66.96	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]
A0A6J1E0X1	1.7e-54	47.56	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DQK2	8.0e-49	46.67	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E266	1.4e-45	42.48	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]

BLAST of ClCG10G015120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	9.1e-29	36.65	Ribonuclease T2 family protein	[more]
AT2G02990.1	1.3e-27	33.17	ribonuclease 1	[more]
AT1G26820.1	8.5e-27	33.98	ribonuclease 3	[more]
AT2G39780.1	1.5e-18	28.88	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 27..207 e-value: 2.4E-38 score: 132.1
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 14..224
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 22..228 e-value: 8.0E-51 score: 175.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 26..223
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 14..224
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..21 score: 6.0
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 27..221 e-value: 2.03781E-55 score: 173.672

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG10G015120.1	ClCG10G015120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding