ClCG10G012100 (gene) Watermelon (Charleston Gray) v2.5

BLAST of ClCG10G012100 vs. NCBI nr
Match: KAG7036600.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1067.4 bits (2759), Expect = 5.4e-308
Identity = 545/744 (73.25%), Postives = 604/744 (81.18%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           MEDE+G  LKVWISIWMCL YCYGVGK+I EG TRF+ IFPVI IFLYLP+ LHSLHLGG
Sbjct: 167 MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 226

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFF+AWLANFKLLLFAAGQGPLAT  +SI  FCA+ASFP+K+KQ P QK+SE A NGG
Sbjct: 227 LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPSQKSSEPANNGG 286

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
           +A +   KS+Q+ HR+SP NYAVKI LVAALVKAY+YTHILPQKL+W  LCFHIYF+L+L
Sbjct: 287 AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 346

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           S AAA++AV+  G++ELLPLFH PYLSTSLQDFWGRRWNL VTGILRP  +RPA S AT+
Sbjct: 347 SFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 406

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           L+G RWAPLAGV  TFLVSA MHELMFFYMSRQWPPPTWEVAAFFVLHG CL  EM+ KR
Sbjct: 407 LIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 466

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFV--- 360
           LWID W+MPPLPAAVS+PVTVGFFFWTCFWLFFPPFINTCKADVRVV+EY IFGA V   
Sbjct: 467 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAGVRRR 526

Query: 361 ------KNNIPNSFWPNFKHIISFN-----------YLKIPL-------------NSIPN 420
                 KN  P  F  +F  I SF+           +L+  L             NSIPN
Sbjct: 527 KGKRKKKNRAP--FLKSFS-ISSFSDSPLMAAEILIFLQTLLQVSSSLCFCFSIRNSIPN 586

Query: 421 GFFKFLLILPFFFLFLYIPLQFQTIHLQGSIGFFIGWLASFKLLLFAFGKGPLCSAAASS 480
           GF KFLLILPFF LFLYIPLQFQT+HLQG+IGFFIGWLASFKLLLFAFGKGPLCSA ASS
Sbjct: 587 GFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSAVASS 646

Query: 481 SLRRFLAVGSLPIEIPDHEPPKDRSNPGPVLPLSFSAKLGFLILTIVAINFKNHFHPNAY 540
           SLRRFL VGSLPI+I DH+P   R +   VLPLS S+KL FL L+I AI  KN+FHPN  
Sbjct: 647 SLRRFLLVGSLPIDIYDHDPASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPNGT 706

Query: 541 LIFLCLVIYFLLEILIGSASALVKATLGVELLPYFDEPYLSDSLQDFWGRRWNLMTSRIL 600
           LI  C VIY LLEI++G  +A+ KATLGVELLP FDEPYLS SLQDFWGRRWNLM SRIL
Sbjct: 707 LIVFCFVIYLLLEIVLGGVAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRIL 766

Query: 601 RLSVYDPCRKLTVGVVGRKPAAIVALTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLH 660
           RLSVYDPCR     +VGRKPAAI+A TATFAVSGLMHELIYFY+GRLAPTWEVT FFL+H
Sbjct: 767 RLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVH 826

Query: 661 GVCLAAEMAIKSGVGGRFRLPGVVSVPLTIGFVVTTSFWLFFPQFLRFKADVRMFEEHAA 712
           G  LA EMA+K+ VG RFR P +V+VPLTIGFV+ TSFWLFFPQF RFK DVRMFEEHAA
Sbjct: 827 GFFLAVEMAVKNAVGERFRPPRIVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAA 886

BLAST of ClCG10G012100 vs. NCBI nr
Match: KAA0048417.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.7e-291
Identity = 511/706 (72.38%), Postives = 566/706 (80.17%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+GNFLKVWIS W+CLSYCYGVGKRIP GITRF+FI PV+S+FLYLP++LHSLHLGG
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLF+AG GPL+ P IS+GTFCAIASFPVKLKQNPLQK SE AIN G
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAIN-G 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
             P   +KSD +RH QSPL+YAVKIFLVAA+VKAYNYTHILPQKL WLFLCFHIYFLLEL
Sbjct: 121 ELP---SKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAAS VA GG+VELLPLFH+PY STSLQDFWGRRWNLVVTGILRPAVF PAASAA++
Sbjct: 181 SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           L G RW P+ GVTATF+VSAVMHELMFFYMSRQWPPPTWEV+AFFV HG CLV EM+VKR
Sbjct: 241 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 300

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           +WI  W M PLP+ V++PVTVGFFFW+CFWLFFPPFIN+CKADVRVVEEYA+FG F    
Sbjct: 301 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF---- 360

Query: 361 IPNSFWPNFKHIISFNYLKIPLNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHLQGSIGF 420
                                                                     GF
Sbjct: 361 ----------------------------------------------------------GF 420

Query: 421 FIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAVGSLPIEIPDHEPPKDRSNPGPVLPL 480
           FIGWLASFKLLLFAFGKGPL S AASSSLRRFLA+GSLPIEIP    P D   P P+LP 
Sbjct: 421 FIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSD---PNPILPH 480

Query: 481 SFSAKLGFLILTIVAINFKNHFHPNAYLIFLCLVIYFLLEILIGSASALVKATLGVELLP 540
           ++  KLG LILT VA+ F+ + HPN YLI  C +IYFLLEILIG A+A VKATLGVELLP
Sbjct: 481 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 540

Query: 541 YFDEPYLSDSLQDFWGRRWNLMTSRILRLSVYDPCRKLTVGVVGRKPAAIVALTATFAVS 600
           YFDEPYLS SLQDFWG+RWNLM SRILRL+VYDPCRKLT+G+VG+KP+AIVA+TATFAVS
Sbjct: 541 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 600

Query: 601 GLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSGVGGRFRLPGVVSVPLTIGFV 660
           GLMHELIYFYMGR+APTWEVTCFFLLHGV L+AEMA+KS VGGRFR+P +VS   T  FV
Sbjct: 601 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 637

Query: 661 VTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVMADLITQFKFI 707
           +TTS WLFFPQF R K +VRMFEEHAAL AFVK+V   LIT FKF+
Sbjct: 661 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFL 637

BLAST of ClCG10G012100 vs. NCBI nr
Match: XP_038905301.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 696.8 bits (1797), Expect = 1.9e-196
Identity = 338/381 (88.71%), Postives = 358/381 (93.96%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ NFLKVWISIW+CLSYCYGVG+RIP GITRF+FIFPVIS+FLYLP+IL SLHLGG
Sbjct: 1   MEEEIQNFLKVWISIWICLSYCYGVGERIPHGITRFLFIFPVISLFLYLPLILRSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLFAAGQGPLA P +SIGTFCA+ASFPVKLKQNP QKTSEAAINGG
Sbjct: 61  LTAFFIAWLANFKLLLFAAGQGPLAAPGMSIGTFCAVASFPVKLKQNPFQKTSEAAINGG 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
           SA IN T S+ +RHRQS LNY VKI LVAALVKAY+YTHILPQKLLWLFLCFHIYFLLEL
Sbjct: 121 SARINDTNSNHNRHRQSLLNYTVKILLVAALVKAYDYTHILPQKLLWLFLCFHIYFLLEL 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAASAVA GG+VELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAAT+
Sbjct: 181 SLAAAASAVAFGGTVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATK 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           LLG RWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEV AFFV HG CLVAEM VKR
Sbjct: 241 LLGSRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVMAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           LWIDTW+M PLPAAVS+PVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN
Sbjct: 301 LWIDTWQMRPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360

Query: 361 IPNSFWPNFKHIISFNYLKIP 382
           IP+SFW NF + I+FNY+KIP
Sbjct: 361 IPSSFWSNFNNNIAFNYVKIP 381

BLAST of ClCG10G012100 vs. NCBI nr
Match: XP_004149731.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58562.1 hypothetical protein Csa_002341 [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 1.7e-184
Identity = 318/380 (83.68%), Postives = 347/380 (91.32%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           MEDE+GNFLKVWISIW+CLSYCYGVG+RIP GITRF+FIFPVIS+FLYLP++LHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLF+AG GPLA P ISIGTFCAIASFPVKLKQ PLQKTSE AI+GG
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFAIHGG 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
                 +KSD +RHRQSPL+YAVKIFLVA +VKAYNYTHILPQKL WLFLCFHIYFLLEL
Sbjct: 121 LP----SKSDHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAAS VACGG++ELLPLFHQPY STSLQDFWGRRWNLVVTGILRPAVFRPAASAA++
Sbjct: 181 SLAAAASIVACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASK 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           LLG++  PLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEV+AFFV HG CLVAEM VKR
Sbjct: 241 LLGKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           +WI  WRM PLP  VS+PVTVGFFFW+CFWLFFPPFIN+CKADVRVVEEYA+FGAF+KNN
Sbjct: 301 VWIQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNN 360

Query: 361 IPNSFWPNF-KHIISFNYLK 380
           IP + WPN   +II FNY+K
Sbjct: 361 IPINLWPNLNNNIIPFNYVK 376

BLAST of ClCG10G012100 vs. NCBI nr
Match: XP_008461897.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo])

HSP 1 Score: 641.0 bits (1652), Expect = 1.2e-179
Identity = 309/380 (81.32%), Postives = 343/380 (90.26%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+GNFLKVWIS W+CLSYCYGVGKRIP GITRF+FI PV+S+FLYLP++LHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLF+AG GPL+ P IS+GTFCAIASFPVKLKQNPLQK SE AIN G
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAIN-G 168

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
             P   +KSD +RH QSPL+YAVKIFLVAA+VKAYNYTHILPQKL WLFLCFHIYFLLEL
Sbjct: 169 ELP---SKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 228

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAAS VA GG+VELLPLFH+PY STSLQDFWGRRWNLVVTGILRPAVF PAASAA++
Sbjct: 229 SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 288

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           L G RW P+ GVTATF+VSAVMHELMFFYMSRQWPPPTWEV+AFFV HG CLV EM+VKR
Sbjct: 289 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 348

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           +WI  W M PLP+ V++PVTVGFFFW+CFWLFFPPFIN+CKADVRVVEEYA+FGAFVKNN
Sbjct: 349 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNN 408

Query: 361 IPNSFWPNFK-HIISFNYLK 380
           IP+S +PNFK + I+F YLK
Sbjct: 409 IPSSLFPNFKNNNIAFTYLK 424

BLAST of ClCG10G012100 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.2e-71
Identity = 149/354 (42.09%), Postives = 216/354 (61.02%), Query Frame = 0

Query: 5   MGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGGLTAF 64
           M +F+K W  + + L Y + + K +P+GI R +  FPV  IF  +P +++SLHL G+TAF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FIAWLANFKLLLFAAGQGPLAT--PAISIGTFCAIASFPVKLKQNPLQKTSEAAINGGSA 124
           FIAWLANFKLLLFA G+GPL++    +S+  F A++  P+K++ +P  K ++   + GS 
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP--KPTKTHSHEGST 120

Query: 125 PINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLELSL 184
                        + PL Y +K   V  ++KAY Y+  LP+K++      HIYF LE+ L
Sbjct: 121 -------------EGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIIL 180

Query: 185 AAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATRLL 244
           AA A+AV     +EL P F++PYL+TSLQDFWGRRWNL+VTGILRP V+ P+    + +L
Sbjct: 181 AATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VL 240

Query: 245 GRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKRLW 304
           G  ++ +     TF+VS +MHEL+FFYM R    P W++  FF+++G C   E+ +K+  
Sbjct: 241 GPNYSQILAAFGTFVVSGIMHELIFFYMGRL--RPDWKMMWFFLINGFCTTVEIAIKKTI 300

Query: 305 IDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAF 357
              WR    P A+S  +T+ F   T  WLF P F N C    + ++EYA  GAF
Sbjct: 301 NGRWR---FPKAISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

BLAST of ClCG10G012100 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.4e-61
Identity = 141/360 (39.17%), Postives = 198/360 (55.00%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ + +KVW+   + +SYCY    RI  G+ R + + PV  +FL LP+ + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
            TAFF++WLANFKL+LF+  QGPL     ++  F     FP+KL+QNP            
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNP------------ 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
                     + +++     +AVK+     L+  Y Y   +   +L +    HIY   E+
Sbjct: 121 ----------KPQNQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEI 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
            LA     ++     +L P F++PYLSTSLQDFWGRRWNL+V  ILRPAV+ P    A R
Sbjct: 181 LLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGR 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
            +    A   GV A+FLVS V+HEL+FFY +R+   PT EV  FFVLHG C  AE   KR
Sbjct: 241 KMNSDQALFLGVFASFLVSGVVHELIFFYFTRE--SPTGEVTLFFVLHGVCTAAECAAKR 300

Query: 301 L-WIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKN 360
              +  W++  +   VS  +TVGF   T  WLFFP    +   + R+ +E  +F  FVK+
Sbjct: 301 TRLVRRWKVSQM---VSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

BLAST of ClCG10G012100 vs. ExPASy Swiss-Prot
Match: Q4PT07 (Probable long-chain-alcohol O-fatty-acyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=At1g34500 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.2e-61
Identity = 140/360 (38.89%), Postives = 203/360 (56.39%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ NF+ VWIS  + +SYCY +   I  G+ R   + P+  +F  LP+   S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
            TAF+++ +A+ KL+LFA  QGPL   A ++  F     FP+KL++NP            
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAPNLIQFVCFTCFPIKLQRNP------------ 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCF-HIYFLLE 180
                  KS  S++      +A+KI +   ++  YNY+H LPQ +L L LCF H+Y  LE
Sbjct: 121 -------KSQPSQNHFHKRAFAIKIMIFGVVLHVYNYSHFLPQTVL-LSLCFLHLYVELE 180

Query: 181 LSLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAAT 240
           + L      ++     +L P F++PYL+TSLQDFWGRRWNL+V+ +LR  ++ P   A  
Sbjct: 181 ILLGPLKVLLSMALGCDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQ 240

Query: 241 RLLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVK 300
           R +   WA   G   TFLVS + HEL++FY++R+   PTWEV  FFVL+G C   E+ VK
Sbjct: 241 RPMNSGWARFMGYLVTFLVSGLFHELVYFYITRE--TPTWEVTLFFVLNGVCTGTEVAVK 300

Query: 301 R-LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVK 359
           R  ++  W   P+ ++VS  +T+GF   T   LFFP FI +   + R   E   F  FVK
Sbjct: 301 RTAFLQRWW--PVRSSVSRLLTMGFVVVTGGLLFFPLFIRSGMME-RRANETLFFLDFVK 335

BLAST of ClCG10G012100 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 9.3e-61
Identity = 134/336 (39.88%), Postives = 189/336 (56.25%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           M++E+ N +KVW+S  + +SYCY +  RI  G  RF+ + PV+++FL LP+   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           +TAFF+ WLANFKL+LF+  +GPL     +   F     FP+K++QNP            
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQNP------------ 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
                     +S++    L +A+K+ + A L+  Y+Y   L   +L      H+Y  +E+
Sbjct: 121 ----------KSQNHLPKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEI 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
            L      V      +L P  ++PYL+TSLQDFWGRRWNL+V  ILRPAV+ P    + R
Sbjct: 181 ILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSER 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
            +   WA   G+ A F+VS ++HEL+FFY+ R+   PT EV  FFVLHG C   E+ VK+
Sbjct: 241 KMSSGWALFPGILAAFIVSGLVHELLFFYLIREM--PTGEVTLFFVLHGVCTAVELAVKK 300

Query: 301 --LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFP 335
                  WR+ P    VS  +TVGF F T  WLF P
Sbjct: 301 KTTVAQRWRLSP---GVSRVLTVGFVFVTGGWLFTP 309

BLAST of ClCG10G012100 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 8.7e-59
Identity = 138/339 (40.71%), Postives = 188/339 (55.46%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ NF+K+WIS  + +SYCY +   I  G+ R + + PV ++FL  P+    +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
             AFF++WLANFKL+LF+  QGPL+    ++  F  I  FP+K +QNP        I   
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLSPLPRTLSRFICITCFPIKPQQNP-------NIQNY 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
             PI                +A+K+ +   L++ Y Y   L   LL +F   HI+  LE+
Sbjct: 121 KIPI--------------WLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEI 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
                 + V      +L P  ++PYL+TSLQDFWGRRWNL+V  ILRPAV+ PA   A R
Sbjct: 181 VFMLVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACR 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVK- 300
            +    A   GV A FLVS  +HE++FFY++R+   PT EV  FF+LHG C VAE+ VK 
Sbjct: 241 KVNSDQAMFLGVFAAFLVSGAVHEMLFFYLTRE--VPTGEVTWFFLLHGVCTVAEVAVKK 300

Query: 301 ----RLWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFP 335
               R W   WR+ P    VS  +TVGF   T  W FFP
Sbjct: 301 STFVRRW---WRVSP---TVSRLLTVGFVVVTSGWFFFP 310

BLAST of ClCG10G012100 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.3e-291
Identity = 511/706 (72.38%), Postives = 566/706 (80.17%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+GNFLKVWIS W+CLSYCYGVGKRIP GITRF+FI PV+S+FLYLP++LHSLHLGG
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLF+AG GPL+ P IS+GTFCAIASFPVKLKQNPLQK SE AIN G
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAIN-G 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
             P   +KSD +RH QSPL+YAVKIFLVAA+VKAYNYTHILPQKL WLFLCFHIYFLLEL
Sbjct: 121 ELP---SKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAAS VA GG+VELLPLFH+PY STSLQDFWGRRWNLVVTGILRPAVF PAASAA++
Sbjct: 181 SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           L G RW P+ GVTATF+VSAVMHELMFFYMSRQWPPPTWEV+AFFV HG CLV EM+VKR
Sbjct: 241 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 300

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           +WI  W M PLP+ V++PVTVGFFFW+CFWLFFPPFIN+CKADVRVVEEYA+FG F    
Sbjct: 301 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF---- 360

Query: 361 IPNSFWPNFKHIISFNYLKIPLNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHLQGSIGF 420
                                                                     GF
Sbjct: 361 ----------------------------------------------------------GF 420

Query: 421 FIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAVGSLPIEIPDHEPPKDRSNPGPVLPL 480
           FIGWLASFKLLLFAFGKGPL S AASSSLRRFLA+GSLPIEIP    P D   P P+LP 
Sbjct: 421 FIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSD---PNPILPH 480

Query: 481 SFSAKLGFLILTIVAINFKNHFHPNAYLIFLCLVIYFLLEILIGSASALVKATLGVELLP 540
           ++  KLG LILT VA+ F+ + HPN YLI  C +IYFLLEILIG A+A VKATLGVELLP
Sbjct: 481 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 540

Query: 541 YFDEPYLSDSLQDFWGRRWNLMTSRILRLSVYDPCRKLTVGVVGRKPAAIVALTATFAVS 600
           YFDEPYLS SLQDFWG+RWNLM SRILRL+VYDPCRKLT+G+VG+KP+AIVA+TATFAVS
Sbjct: 541 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 600

Query: 601 GLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSGVGGRFRLPGVVSVPLTIGFV 660
           GLMHELIYFYMGR+APTWEVTCFFLLHGV L+AEMA+KS VGGRFR+P +VS   T  FV
Sbjct: 601 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 637

Query: 661 VTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVMADLITQFKFI 707
           +TTS WLFFPQF R K +VRMFEEHAAL AFVK+V   LIT FKF+
Sbjct: 661 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFL 637

BLAST of ClCG10G012100 vs. ExPASy TrEMBL
Match: A0A0A0LD00 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684680 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 8.1e-185
Identity = 318/380 (83.68%), Postives = 347/380 (91.32%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           MEDE+GNFLKVWISIW+CLSYCYGVG+RIP GITRF+FIFPVIS+FLYLP++LHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLF+AG GPLA P ISIGTFCAIASFPVKLKQ PLQKTSE AI+GG
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFAIHGG 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
                 +KSD +RHRQSPL+YAVKIFLVA +VKAYNYTHILPQKL WLFLCFHIYFLLEL
Sbjct: 121 LP----SKSDHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAAS VACGG++ELLPLFHQPY STSLQDFWGRRWNLVVTGILRPAVFRPAASAA++
Sbjct: 181 SLAAAASIVACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASK 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           LLG++  PLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEV+AFFV HG CLVAEM VKR
Sbjct: 241 LLGKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           +WI  WRM PLP  VS+PVTVGFFFW+CFWLFFPPFIN+CKADVRVVEEYA+FGAF+KNN
Sbjct: 301 VWIQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNN 360

Query: 361 IPNSFWPNF-KHIISFNYLK 380
           IP + WPN   +II FNY+K
Sbjct: 361 IPINLWPNLNNNIIPFNYVK 376

BLAST of ClCG10G012100 vs. ExPASy TrEMBL
Match: A0A1S3CFN5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500392 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 6.0e-180
Identity = 309/380 (81.32%), Postives = 343/380 (90.26%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+GNFLKVWIS W+CLSYCYGVGKRIP GITRF+FI PV+S+FLYLP++LHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFFIAWLANFKLLLF+AG GPL+ P IS+GTFCAIASFPVKLKQNPLQK SE AIN G
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAIN-G 168

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
             P   +KSD +RH QSPL+YAVKIFLVAA+VKAYNYTHILPQKL WLFLCFHIYFLLEL
Sbjct: 169 ELP---SKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 228

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
           SLAAAAS VA GG+VELLPLFH+PY STSLQDFWGRRWNLVVTGILRPAVF PAASAA++
Sbjct: 229 SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 288

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
           L G RW P+ GVTATF+VSAVMHELMFFYMSRQWPPPTWEV+AFFV HG CLV EM+VKR
Sbjct: 289 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 348

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
           +WI  W M PLP+ V++PVTVGFFFW+CFWLFFPPFIN+CKADVRVVEEYA+FGAFVKNN
Sbjct: 349 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNN 408

Query: 361 IPNSFWPNFK-HIISFNYLK 380
           IP+S +PNFK + I+F YLK
Sbjct: 409 IPSSLFPNFKNNNIAFTYLK 424

BLAST of ClCG10G012100 vs. ExPASy TrEMBL
Match: A0A498IVH7 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_016895 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 5.5e-173
Identity = 341/723 (47.16%), Postives = 447/723 (61.83%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME E+GNF+KVW+S+++ L YCY + K +P+G+ R + I P++S+FLYLP+ L SLHLGG
Sbjct: 1   MEGEIGNFVKVWLSVYLSLCYCYAIAKFVPKGVPRLLLIIPIVSLFLYLPLHLSSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLAT-PAISIGTFCAIASFPVKLKQNPLQKTSEAAING 120
            TAFFI+WLANFKLLLFA G+GPL + P+IS+  F A+A  P+K++QN   KT       
Sbjct: 61  TTAFFISWLANFKLLLFAFGKGPLCSDPSISLLRFVAVACLPIKIQQNQYSKTQNKENPA 120

Query: 121 GSAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLE 180
                N TK        SPLNYAVK  L A LVK Y+Y   +   LLW+  CFHIYF LE
Sbjct: 121 PKITKNGTK--------SPLNYAVKGLLFALLVKLYDYKEHIHPNLLWIMYCFHIYFALE 180

Query: 181 LSLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAAT 240
           + L  AA+       +EL P F +PYLS+SLQDFWGRRWN++VT ILRP V+ P  + AT
Sbjct: 181 IILVIAATLARALLGLELEPQFDEPYLSSSLQDFWGRRWNIMVTSILRPTVYEPILALAT 240

Query: 241 RLLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVK 300
           R++GRRWA LAGV  TF+VSA+MHEL+F+Y+ R    PTWE+  FF+LHGACLVAE+ VK
Sbjct: 241 RVVGRRWATLAGVMGTFVVSAIMHELIFYYLGR--TRPTWEITWFFLLHGACLVAEISVK 300

Query: 301 RLWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKN 360
           +  +   +   LP +VS P+TV F   T FWLFFPP +  CKADVR +EEYA  GAFVKN
Sbjct: 301 KA-VGKRKKLQLPRSVSGPLTVAFVMATVFWLFFPPLLR-CKADVRALEEYAAVGAFVKN 360

Query: 361 -------------NIPNSFWPNFKHIISFNYLKIPLNSIPNGFFKFLLILPFFFLFLYIP 420
                         IP  F      + S +Y     N    G  + L +LP  F F Y+P
Sbjct: 361 IGHAFASTAKLEGEIPIFFMVWILVVASLSYCHTVGNVTSPGTTRLLAVLPVVFFFFYLP 420

Query: 421 LQFQTIHLQGSIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAVGSLPIEIPD-- 480
           L   TI L G   FF+ WLA+FKLLLFAF KGPL +    +S+  F+ +  LPI+  +  
Sbjct: 421 LNLTTIFLGGPSSFFLAWLANFKLLLFAFNKGPL-ATTPPTSISHFIPLACLPIKFRNDP 480

Query: 481 -----HEPPKDRSNPGPVLPLSFSAKLGFLILTIV------AINFKNHFHPNAYLIFLCL 540
                 E P  R +    LP    + L ++   ++        + K   HP   L    +
Sbjct: 481 KNHEKKERPAQRISASKSLPKGQKSTLNYVTKALIFSTSVHVYHNKQFIHPKILLFLYSI 540

Query: 541 VIYFLLEILIGSASALVKATLGVELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLSVYD 600
            +Y  LE+++    +L +  LGV   P FDEPYL+ SLQDFWG RWN+M SRIL  +VY 
Sbjct: 541 YMYTGLELILALVGSLARVFLGVNFEPQFDEPYLATSLQDFWGSRWNIMVSRILHPTVYQ 600

Query: 601 PCRKLTVGVVGRKPAAIVALTATFAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAA 660
           P R+++ G +G K A I A+ A+F VS +MHELI++Y+GR  PTWE+TCFFLLHG+CL+ 
Sbjct: 601 PVREISTGALGTKWAPIPAVIASFFVSAVMHELIFYYIGRTLPTWELTCFFLLHGICLSI 660

Query: 661 EMAIKSGVGGRFRLPGVVSVPLTIGFVVTTSFWLFFPQFL-RFKADVRMFEEHAALGAFV 696
           E+ IK    G++RLP  VS PL + FVV T  WLF P  + R + DV  + E+ A   F+
Sbjct: 661 EIVIKKAFDGKWRLPAAVSGPLAMAFVVATGVWLFLPSIMVRCEPDVMAYREYTAFTEFM 710

BLAST of ClCG10G012100 vs. ExPASy TrEMBL
Match: A0A6J1DI64 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020278 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 4.1e-168
Identity = 360/701 (51.36%), Postives = 428/701 (61.06%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           MEDE+ NF KVW S+W+ L YCYGVGK I  G  R +F FPVIS+FLYLPI LHSLHLGG
Sbjct: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGTGRLIFFFPVISLFLYLPINLHSLHLGG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           LTAFF+AWLANFKLLLFA  QGPLA P ISIG FCA+AS P+K  QNPL+K SE   NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENC-NGG 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
           +      KS    H +S LNYAV    +  +V         PQ      L  HI   L  
Sbjct: 121 APMEAIIKS--HHHHESLLNYAVHWDSLPVIV---------PQ------LPNHISNPLH- 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
                       G  E++ +       TSL        N++  G+ + ++F    S    
Sbjct: 181 ------------GKEEIMSI-------TSL--------NMMADGLAKVSIFVNQFS---- 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
                                                       ++LH    V E   + 
Sbjct: 241 -------------------------------------------IYLLHICVCVEEKEEED 300

Query: 301 LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360
                +++ PL A       +G F  T                V +V     F   ++  
Sbjct: 301 EKKSKFQITPLMA-----TEIGIFVQT----------------VLLVCSSLCFCFSIR-- 360

Query: 361 IPNSFWPNFKHIISFNYLKIPLNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHLQGSIGF 420
                                 NSIP+GF +FLLILPFFFLFL IPLQF TIHLQG+IGF
Sbjct: 361 ----------------------NSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGF 420

Query: 421 FIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAVGSLPIEIPDHEPPKDRSNPGPVLPL 480
           FIGWL SFKLLLFAFGKGPLCSA A++SLRRFLAVGSLPIEIP H P     +  PVL L
Sbjct: 421 FIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPQAPAPDSPPVLSL 480

Query: 481 SFSAKLGFLILTIVAINFKNHFHPNAYLIFLCLVIYFLLEILIGSASALVKATLGVELLP 540
           +F AKLG L+LT  AI F+N+F+P A L+F CL+IY LLEIL+G A+AL +  LGV+L P
Sbjct: 481 TFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGVDLSP 540

Query: 541 YFDEPYLSDSLQDFWGRRWNLMTSRILRLSVYDPCRKLTVGVVGRKPAAIVALTATFAVS 600
           YFDEPYLS SLQ+FWGRRWNLMT+RILR++VYDPCR++    VG++ A ++A+ ATFAVS
Sbjct: 541 YFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAATFAVS 563

Query: 601 GLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSGVGGRFRLPGVVSVPLTIGFV 660
           GLMHELIYFYMGR+APTWEVTCFFLLHGVCL AEMA+K+  GGRFRLP  VS PLT+GFV
Sbjct: 601 GLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLTLGFV 563

Query: 661 VTTSFWLFFPQFLRFKADVRMFEEHAALGAFVKSVMADLIT 702
           + TSFWLFFPQFLRFKADVRMFEE+AALGA VK+V  D+IT
Sbjct: 661 MVTSFWLFFPQFLRFKADVRMFEEYAALGALVKNVTGDMIT 563

BLAST of ClCG10G012100 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 272.7 bits (696), Expect = 8.3e-73
Identity = 149/354 (42.09%), Postives = 216/354 (61.02%), Query Frame = 0

Query: 5   MGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGGLTAF 64
           M +F+K W  + + L Y + + K +P+GI R +  FPV  IF  +P +++SLHL G+TAF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FIAWLANFKLLLFAAGQGPLAT--PAISIGTFCAIASFPVKLKQNPLQKTSEAAINGGSA 124
           FIAWLANFKLLLFA G+GPL++    +S+  F A++  P+K++ +P  K ++   + GS 
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP--KPTKTHSHEGST 120

Query: 125 PINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLELSL 184
                        + PL Y +K   V  ++KAY Y+  LP+K++      HIYF LE+ L
Sbjct: 121 -------------EGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIIL 180

Query: 185 AAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATRLL 244
           AA A+AV     +EL P F++PYL+TSLQDFWGRRWNL+VTGILRP V+ P+    + +L
Sbjct: 181 AATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VL 240

Query: 245 GRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKRLW 304
           G  ++ +     TF+VS +MHEL+FFYM R    P W++  FF+++G C   E+ +K+  
Sbjct: 241 GPNYSQILAAFGTFVVSGIMHELIFFYMGRL--RPDWKMMWFFLINGFCTTVEIAIKKTI 300

Query: 305 IDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAF 357
              WR    P A+S  +T+ F   T  WLF P F N C    + ++EYA  GAF
Sbjct: 301 NGRWR---FPKAISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

BLAST of ClCG10G012100 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.7e-62
Identity = 141/360 (39.17%), Postives = 198/360 (55.00%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ + +KVW+   + +SYCY    RI  G+ R + + PV  +FL LP+ + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
            TAFF++WLANFKL+LF+  QGPL     ++  F     FP+KL+QNP            
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNP------------ 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
                     + +++     +AVK+     L+  Y Y   +   +L +    HIY   E+
Sbjct: 121 ----------KPQNQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEI 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
            LA     ++     +L P F++PYLSTSLQDFWGRRWNL+V  ILRPAV+ P    A R
Sbjct: 181 LLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGR 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
            +    A   GV A+FLVS V+HEL+FFY +R+   PT EV  FFVLHG C  AE   KR
Sbjct: 241 KMNSDQALFLGVFASFLVSGVVHELIFFYFTRE--SPTGEVTLFFVLHGVCTAAECAAKR 300

Query: 301 L-WIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKN 360
              +  W++  +   VS  +TVGF   T  WLFFP    +   + R+ +E  +F  FVK+
Sbjct: 301 TRLVRRWKVSQM---VSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

BLAST of ClCG10G012100 vs. TAIR 10
Match: AT1G34500.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 238.0 bits (606), Expect = 2.3e-62
Identity = 140/360 (38.89%), Postives = 203/360 (56.39%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ NF+ VWIS  + +SYCY +   I  G+ R   + P+  +F  LP+   S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
            TAF+++ +A+ KL+LFA  QGPL   A ++  F     FP+KL++NP            
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAPNLIQFVCFTCFPIKLQRNP------------ 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCF-HIYFLLE 180
                  KS  S++      +A+KI +   ++  YNY+H LPQ +L L LCF H+Y  LE
Sbjct: 121 -------KSQPSQNHFHKRAFAIKIMIFGVVLHVYNYSHFLPQTVL-LSLCFLHLYVELE 180

Query: 181 LSLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAAT 240
           + L      ++     +L P F++PYL+TSLQDFWGRRWNL+V+ +LR  ++ P   A  
Sbjct: 181 ILLGPLKVLLSMALGCDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQ 240

Query: 241 RLLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVK 300
           R +   WA   G   TFLVS + HEL++FY++R+   PTWEV  FFVL+G C   E+ VK
Sbjct: 241 RPMNSGWARFMGYLVTFLVSGLFHELVYFYITRE--TPTWEVTLFFVLNGVCTGTEVAVK 300

Query: 301 R-LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVK 359
           R  ++  W   P+ ++VS  +T+GF   T   LFFP FI +   + R   E   F  FVK
Sbjct: 301 RTAFLQRWW--PVRSSVSRLLTMGFVVVTGGLLFFPLFIRSGMME-RRANETLFFLDFVK 335

BLAST of ClCG10G012100 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 236.5 bits (602), Expect = 6.6e-62
Identity = 134/336 (39.88%), Postives = 189/336 (56.25%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           M++E+ N +KVW+S  + +SYCY +  RI  G  RF+ + PV+++FL LP+   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
           +TAFF+ WLANFKL+LF+  +GPL     +   F     FP+K++QNP            
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQNP------------ 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
                     +S++    L +A+K+ + A L+  Y+Y   L   +L      H+Y  +E+
Sbjct: 121 ----------KSQNHLPKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEI 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
            L      V      +L P  ++PYL+TSLQDFWGRRWNL+V  ILRPAV+ P    + R
Sbjct: 181 ILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSER 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVKR 300
            +   WA   G+ A F+VS ++HEL+FFY+ R+   PT EV  FFVLHG C   E+ VK+
Sbjct: 241 KMSSGWALFPGILAAFIVSGLVHELLFFYLIREM--PTGEVTLFFVLHGVCTAVELAVKK 300

Query: 301 --LWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFP 335
                  WR+ P    VS  +TVGF F T  WLF P
Sbjct: 301 KTTVAQRWRLSP---GVSRVLTVGFVFVTGGWLFTP 309

BLAST of ClCG10G012100 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 229.9 bits (585), Expect = 6.2e-60
Identity = 138/339 (40.71%), Postives = 188/339 (55.46%), Query Frame = 0

Query: 1   MEDEMGNFLKVWISIWMCLSYCYGVGKRIPEGITRFVFIFPVISIFLYLPIILHSLHLGG 60
           ME+E+ NF+K+WIS  + +SYCY +   I  G+ R + + PV ++FL  P+    +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  LTAFFIAWLANFKLLLFAAGQGPLATPAISIGTFCAIASFPVKLKQNPLQKTSEAAINGG 120
             AFF++WLANFKL+LF+  QGPL+    ++  F  I  FP+K +QNP        I   
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLSPLPRTLSRFICITCFPIKPQQNP-------NIQNY 120

Query: 121 SAPINYTKSDQSRHRQSPLNYAVKIFLVAALVKAYNYTHILPQKLLWLFLCFHIYFLLEL 180
             PI                +A+K+ +   L++ Y Y   L   LL +F   HI+  LE+
Sbjct: 121 KIPI--------------WLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEI 180

Query: 181 SLAAAASAVACGGSVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATR 240
                 + V      +L P  ++PYL+TSLQDFWGRRWNL+V  ILRPAV+ PA   A R
Sbjct: 181 VFMLVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACR 240

Query: 241 LLGRRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVAAFFVLHGACLVAEMMVK- 300
            +    A   GV A FLVS  +HE++FFY++R+   PT EV  FF+LHG C VAE+ VK 
Sbjct: 241 KVNSDQAMFLGVFAAFLVSGAVHEMLFFYLTRE--VPTGEVTWFFLLHGVCTVAEVAVKK 300

Query: 301 ----RLWIDTWRMPPLPAAVSIPVTVGFFFWTCFWLFFP 335
               R W   WR+ P    VS  +TVGF   T  W FFP
Sbjct: 301 STFVRRW---WRVSP---TVSRLLTVGFVVVTSGWFFFP 310