ClCG10G006350 (gene) Watermelon (Charleston Gray) v2.5

BLAST of ClCG10G006350 vs. NCBI nr
Match: KAG7015479.1 (Vacuolar protein sorting-associated protein 51-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 646/686 (94.17%), Postives = 661/686 (96.36%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFK 
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKV 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAEDLSS LVIGSSKDGN SE VYGASHEASVREFAEA+RAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASVREFAEAIRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDA EQFI+KQICAADLLRVFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSLQLALEASKKALLQGSM 360
           LKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSLQLALEASKKA+LQGSM
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSM 436

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           DVLLDFRQLL DQSGL I+ RDSIVDWVQEGFQDFF+ALV RFMLLSGKN+SFS+SQVL 
Sbjct: 437 DVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLP 496

Query: 421 EATQAEKVIAGLVL------EIAASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKLLHLY 480
           EATQAEKV+AGLVL      EIAASFSGGG RGYEYGPAFVPAEICRMFRAAGEK LHLY
Sbjct: 497 EATQAEKVVAGLVLVLAQVSEIAASFSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLY 556

Query: 481 INMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQVLPQGTRKHR 540
           INMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEA+GSEVKQ+LPQGTRKHR
Sbjct: 557 INMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAIGSEVKQILPQGTRKHR 616

Query: 541 RADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVL 600
           R DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSV+
Sbjct: 617 RTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVV 676

Query: 601 TTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAA 660
           TTAVKLSLKTL EFVRLQTFNRSGFQQ+QLDMQFLRTPLKEIADDEAAIDFLLDEVIVAA
Sbjct: 677 TTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAA 736

Query: 661 SERCLDPIPLEPPILDKLIQAKLAKA 680
           SERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 SERCLDAIPLEPPILDKLIQAKLAKA 762

BLAST of ClCG10G006350 vs. NCBI nr
Match: XP_038887792.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887793.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887794.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887795.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887796.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 645/698 (92.41%), Postives = 660/698 (94.56%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEE IA+VLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEVIAVVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQ+NAEDLSS LV GSSKDG+ SELVYGASHEASVREFAEAVRAYRVIF+D
Sbjct: 257 EKLEQSTLDLQVNAEDLSSALVNGSSKDGSTSELVYGASHEASVREFAEAVRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDATEQFIKKQICAADLL VFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDATEQFIKKQICAADLLLVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSMD 360
           LKAAQVAVKQYV   FSRLLQDISDALTQVHTRKKEG QEYSLQLALEA+KKA+LQGSMD
Sbjct: 377 LKAAQVAVKQYVKCKFSRLLQDISDALTQVHTRKKEGAQEYSLQLALEATKKAVLQGSMD 436

Query: 361 VLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLTE 420
           VLLDFRQLL D+SGLIIN RDSIVDWVQEGFQDFF+ALV RFMLLSGKNNS+S+SQVLTE
Sbjct: 437 VLLDFRQLLEDKSGLIINQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNNSYSQSQVLTE 496

Query: 421 ATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICRM 480
           ATQAEKVIAGLVL                   EIAASFSGGGIRGYEYGPAFVPAEICRM
Sbjct: 497 ATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFVPAEICRM 556

Query: 481 FRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 540
           FRAAGEK LHLYINMRSQRIS+L TKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV
Sbjct: 557 FRAAGEKFLHLYINMRSQRISILSTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 616

Query: 541 KQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIEI 600
           KQ+LPQGTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIEI
Sbjct: 617 KQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEI 676

Query: 601 FTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA 660
           FTRVEFTQGSV+TT VKLSLKTLQEFVR QTFNRSGFQQIQLDMQFLRTPLKEIADDEAA
Sbjct: 677 FTRVEFTQGSVVTTLVKLSLKTLQEFVRFQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA 736

Query: 661 IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 IDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKA 774

BLAST of ClCG10G006350 vs. NCBI nr
Match: XP_023552385.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552386.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552387.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552388.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 646/699 (92.42%), Postives = 661/699 (94.56%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFK 
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKV 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAEDLSS LVIGSSKDGN SE VYGASHEASVREFAEA+RAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAEDLSSALVIGSSKDGNASESVYGASHEASVREFAEAIRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDA EQFI+KQICAADLLRVFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSLQLALEASKKALLQGSM 360
           LKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSLQLALEASKKA+LQGSM
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSM 436

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           DVLLDFRQLL DQSGL I+ RDSIVDWVQEGFQDFF+ALV RFMLLSGKN+SFS+SQVL 
Sbjct: 437 DVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLP 496

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           EATQAEKV+AGLVL                   EIAASFSGGG RGYEYGPAFVPAEICR
Sbjct: 497 EATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGTRGYEYGPAFVPAEICR 556

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           MFRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEA+GSE
Sbjct: 557 MFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAIGSE 616

Query: 541 VKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIE 600
           VKQ+LPQGTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIE
Sbjct: 617 VKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIE 676

Query: 601 IFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEA 660
           IFTRVEFTQGSV+TTAVKLSLKTL EFVRLQTFNRSGFQQ+QLDMQFLRTPLKEIADDEA
Sbjct: 677 IFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEA 736

Query: 661 AIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           AIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 AIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKA 775

BLAST of ClCG10G006350 vs. NCBI nr
Match: KAG6577400.1 (Vacuolar protein sorting-associated protein 51-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 646/699 (92.42%), Postives = 661/699 (94.56%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFK 
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKV 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAEDLSS LVIGSSKDGN SE VYGASHEASVREFAEA+RAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASVREFAEAIRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDA EQFI+KQICAADLLRVFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSLQLALEASKKALLQGSM 360
           LKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSLQLALEASKKA+LQGSM
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSM 436

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           DVLLDFRQLL DQSGL I+ RDSIVDWVQEGFQDFF+ALV RFMLLSGKN+SFS+SQVL 
Sbjct: 437 DVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLP 496

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           EATQAEKV+AGLVL                   EIAASFSGGG RGYEYGPAFVPAEICR
Sbjct: 497 EATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGTRGYEYGPAFVPAEICR 556

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           MFRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEA+GSE
Sbjct: 557 MFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAIGSE 616

Query: 541 VKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIE 600
           VKQ+LPQGTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIE
Sbjct: 617 VKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIE 676

Query: 601 IFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEA 660
           IFTRVEFTQGSV+TTAVKLSLKTL EFVRLQTFNRSGFQQ+QLDMQFLRTPLKEIADDEA
Sbjct: 677 IFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEA 736

Query: 661 AIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           AIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 AIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKA 775

BLAST of ClCG10G006350 vs. NCBI nr
Match: XP_022929381.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929382.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929383.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929384.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 647/699 (92.56%), Postives = 661/699 (94.56%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFK 
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKV 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAEDLSS LVIGSSKDGN SE VYGASHEASVREFAEA+RAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASVREFAEAIRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDATEQFI+KQICAADLLRVFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSLQLALEASKKALLQGSM 360
           LKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSLQLALEASKKA+LQGSM
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSM 436

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           DVLLDFRQLL DQSGL I+ RDSIVDWVQEGFQDFF+ALV RFMLLSGKN+SFS+SQVL 
Sbjct: 437 DVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLP 496

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           EATQAEKV+AGLVL                   EIAASFSGGG RGYEYGPAFVPAEICR
Sbjct: 497 EATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGTRGYEYGPAFVPAEICR 556

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           MFRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE
Sbjct: 557 MFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 616

Query: 541 VKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIE 600
           VKQ+LPQGTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIE
Sbjct: 617 VKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIE 676

Query: 601 IFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEA 660
           IFTRVEFTQGSV+TTAVKLSLKTL EFVRLQTFNRSGFQQ+QLDMQFLRTPLKEIADDEA
Sbjct: 677 IFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEA 736

Query: 661 AIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           AIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 AIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKA 775

BLAST of ClCG10G006350 vs. ExPASy Swiss-Prot
Match: Q0WQ75 (Vacuolar protein sorting-associated protein 51 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS51 PE=1 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 5.5e-250
Identity = 468/702 (66.67%), Postives = 558/702 (79.49%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDG
Sbjct: 73  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDG 132

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI K 
Sbjct: 133 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKV 192

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLKAKLL
Sbjct: 193 YGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLL 252

Query: 181 EKLEQSTLDLQLNAEDLSSELV-IGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFS 240
           EKLEQS   LQ+  E+ S+ +    SS D   ++      HE +VR F+EA+RAYR IF 
Sbjct: 253 EKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFP 312

Query: 241 DSDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDY 300
           DS+ +L KLA+ L   HF+  E +IKK++ AAD L +F I+W DV+L+ EVL +A L D 
Sbjct: 313 DSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDL 372

Query: 301 SLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSM 360
           S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L++ LEAS+KA+LQG+ 
Sbjct: 373 SAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTT 432

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           ++  DFRQLL +++G+ I ++D I  W+Q+G QDFF++L A+F++LSGK +S ++     
Sbjct: 433 NIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSND----I 492

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           E   ++K+ AGL+L                   EIAASFSGG  + +E GPAF+P E+CR
Sbjct: 493 EGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCR 552

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           +F AA EKLL  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVHM+VD+FL ELE VG E
Sbjct: 553 VFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKE 612

Query: 541 VKQVLPQGT-RKHRRADSNGS--TTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQ 600
           VKQVLPQGT RKH+R DSNGS  TTSSRSN L  +K+ +SN+QRARSQL ETHLAKLFKQ
Sbjct: 613 VKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQ 672

Query: 601 KIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIAD 660
           K+EIFT+VEFTQ SV+TT VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+ PLKE  +
Sbjct: 673 KVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVE 732

Query: 661 DEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+
Sbjct: 733 DEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKS 770

BLAST of ClCG10G006350 vs. ExPASy Swiss-Prot
Match: Q54KG3 (Vacuolar protein sorting-associated protein 51 homolog OS=Dictyostelium discoideum OX=44689 GN=vps51 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 7.6e-58
Identity = 197/722 (27.29%), Postives = 349/722 (48.34%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           M +EI+ LD D++ LVY+NY KFI+ATD IK+M  N+  ME  M  L + +  + + S+ 
Sbjct: 208 MVSEIRTLDGDMKTLVYDNYTKFINATDIIKKMKTNVENMEEGMALLSKNMDLITNCSEK 267

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           +N++L  +R+ I++L   +   +K+QF+  LP+ L  C+  +AY  AVR+Y     I K 
Sbjct: 268 INSTLSVRRDRIDQLSGLQKFFQKLQFLTALPSSLNHCLAMQAYNQAVRYYNSNSGILKQ 327

Query: 121 YGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKL 180
           Y    SFQ+ +   +  +  +   L E+L S S S     E+A +L  L  PV+ +++K 
Sbjct: 328 YSHIPSFQNIQNECDSIMKTMKDKLYERLSSLSTSQTDCVESAEVLMDLLEPVELVRSKY 387

Query: 181 LEKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIF- 240
           LE  +  T+ L  N E  S E +    K+ N + L           E++  + +Y+ +F 
Sbjct: 388 LESRKHHTITLLENLEKKSVEHITDYIKELNANFL----------SEYSYNITSYKSLFI 447

Query: 241 -----SDSDR-------QLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLL 300
                SDS +       QL   ++DL  K+ +  +  +       + +    II  DV  
Sbjct: 448 NRLDGSDSKKERQQSLLQLEDFSKDLFNKYLNIAKSKLSSFKDPIEKIMALEIINSDVSR 507

Query: 301 LGEVLNDAGLPDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHT----RKKEGVQEYS 360
           LG  L+     +         V   + F F  L + I + + Q+++    R+ E ++ ++
Sbjct: 508 LGSELSS---HEKITNIINSTVHDQIDFYFDNLQKTIKEHIHQLNSTLNERRDEVLEGHN 567

Query: 361 LQLALEASKKALLQGSMDVLLDFRQL----LGDQSGLIINLRDSIVDWVQEGFQDFFKAL 420
           LQ   +A+ KA++    D++L F  L    L  ++  + + +D+I   +Q   Q FF  L
Sbjct: 568 LQELSDATSKAIVN---DIILLFANLKPFFLPTETQFLSSYQDTIFTKIQVKLQQFFLFL 627

Query: 421 VARFML-------LSGKNNSFSESQVLTEAT---QAEKVIAGLVLEIAASFSGGGIRGYE 480
           V    L        +     FS   +L  ++     E     LV+++ + F     +G +
Sbjct: 628 VNIHFLEYLDIIATTSNREQFSGRFLLVLSSICLYFENKGITLVVQLMSEFITVAKQGAK 687

Query: 481 YGP---AFVPAEICRMFRAAGEKLLHLYINMRSQRISVLLTKRFRT---PNWVKHKEPRE 540
                 +F   ++C+  R  G ++L+++  + SQ++  +L K   +    NW+  KEPR+
Sbjct: 688 KELNVFSFNAPDLCKRVRETGLQILNVFTRLSSQKLDKILKKGLESIPNNNWLVLKEPRD 747

Query: 541 VHMFVDLFLQELEAVGSEVKQVLP------QGTRKHRRADSNGSTTSSRSNPLREEKLNK 600
           V    D++L+E+    +E  ++LP         + H R  S G++ SS ++       ++
Sbjct: 748 VRSVNDIYLEEILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNSVSSNNSSSNTPDGHR 807

Query: 601 SNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRS 660
            N   + S         LF++K++    V+F   SVL   +KLSLK+  E +RL+TF  +
Sbjct: 808 RNPSASSSSSSNNTNTTLFEKKVD---HVDFNTLSVLIAIIKLSLKSFNESLRLKTFGTN 867

Query: 661 GFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKL 679
           G  QIQ+D+ +L+  L ++     + D LL E     +ERC+DP+PL   I+ K+ + K+
Sbjct: 868 GCHQIQIDLHYLKLSLYDLFGSN-SFDNLLQECENTVTERCVDPLPLAKSIVSKICETKM 909

BLAST of ClCG10G006350 vs. ExPASy Swiss-Prot
Match: Q3UVL4 (Vacuolar protein sorting-associated protein 51 homolog OS=Mus musculus OX=10090 GN=Vps51 PE=1 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 2.1e-55
Identity = 189/699 (27.04%), Postives = 334/699 (47.78%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   +  + + S  
Sbjct: 93  MVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATNMAVITNFSAR 152

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  AVR+   A  + + 
Sbjct: 153 ISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVLQQ 212

Query: 121 YGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKL 180
           Y    SF+  +   +   A + + L+++          +AE   LL  L  P + L  + 
Sbjct: 213 YQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALGEPAEELCEEF 272

Query: 181 LE----KLEQ--STLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRA 240
           L     +LE+  S+L+ +L     + +++  + + GNG            V    +   A
Sbjct: 273 LAHARGRLEEELSSLEAELGPSPPAPDVLEFTDRGGNG-----------FVGGLCQVAAA 332

Query: 241 YRVIFSDSD----RQLIKLAQDLITKHFDATEQFIKKQICAAD---LLRVFGIIWIDVLL 300
           Y+ +F+        +L   AQ+L  ++F   E+ + ++   +D   L+R        +  
Sbjct: 333 YQELFAAQGPAGAEKLAAFAQELGGRYFALVERRLAQEQGGSDNSLLVRALDRFHRRLRA 392

Query: 301 LGEVLNDAGLPDYSLKAAQVAVKQYVTF-------TFSRLLQDISDALTQVHTRKKEGVQ 360
            G +L  AGL + + +  +   ++ ++         F   L D+  AL       KEG  
Sbjct: 393 PGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLSSLTDVRQALAAPRLAGKEGPS 452

Query: 361 EYSLQLALEASKKALLQGSMDVLLDFRQLLGDQSGLIINLRDSIVDWVQEG-FQDFFKAL 420
              L   + +S  + ++ S+  +  F       S       +     V+EG    F +++
Sbjct: 453 LAELLANVASSILSHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVREGLIVGFIRSM 512

Query: 421 ---VARFMLLSGKNNSFSESQVLTEATQAEKVIAGLVLEIAASFSGGGIRGYEYGPAFVP 480
                 F    G+    +   +L   ++         +    + +       +  P    
Sbjct: 513 CQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQFLVQDQSPVTPV 572

Query: 481 AEICRMFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELE 540
           + +C   R    +LL  Y+ ++   IS +L K   T +W+   EPR V   +   +++  
Sbjct: 573 STLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLSTLEPRNVRAVMKRVVEDTT 632

Query: 541 AVGSEVKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQL-LETHLAKL 600
           A+  +V  +  +G RK + +DS+  T S  S+  ++ +   S T  A     L +++ KL
Sbjct: 633 AIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPSYTPSAPMDTNLLSNIQKL 692

Query: 601 FKQKIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKE 660
           F ++I++F+ VEF + SVLT  +K+SLKTL E VRL+TF R G QQ+Q+D  FL+  L  
Sbjct: 693 FSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVRLRTFGRFGLQQVQVDCHFLQLYLWR 752

Query: 661 IADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQ 674
              DE  +  LLDEV+ +A+ RC DP+P+EP +++ + +
Sbjct: 753 FVADEELVHLLLDEVVASAALRCPDPVPMEPSVVEVICE 780

BLAST of ClCG10G006350 vs. ExPASy Swiss-Prot
Match: Q155U0 (Vacuolar protein sorting-associated protein 51 homolog OS=Danio rerio OX=7955 GN=vps51 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 6.0e-55
Identity = 211/771 (27.37%), Postives = 351/771 (45.53%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           M  +I++LD+D+Q LVYENYNKFISATDTI++M N+   ME  M+ L   + ++   S  
Sbjct: 76  MVKQIRSLDSDMQTLVYENYNKFISATDTIRKMKNDFKKMEDEMDCLSANMAAITEFSAR 135

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           ++ +L ++   I KL     LLRK+QF+++LPARL KC++ +AYA AV  +  A  + + 
Sbjct: 136 ISGTLQDQHAQITKLSGVHTLLRKLQFLFELPARLNKCLELQAYAQAVSSHRRARCVLQQ 195

Query: 121 YGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKL 180
           Y    SF+  +      +  + + L++K      S +  +E   LL QLD P + L  K 
Sbjct: 196 YSHMPSFRGIQDDCHVIMEQLAQQLRQKFRDGGSSAKDLSECVELLLQLDEPAEELCDKF 255

Query: 181 L----EKLEQSTLDLQLNAEDLS-SELVIGS-SKDGNGSELVYGASHEAS---------- 240
           L     +LE     L+   +D + ++   GS  K   GS  V  +S  ++          
Sbjct: 256 LSHAQSRLEADLQGLEAELKDSAVTDTGAGSVQKTSPGSNPVSPSSSVSNPFLSPAAGTD 315

Query: 241 VREFAE------------AVRAYRVIFSD---------------SDRQLIKLAQDLITKH 300
           + EF +             + +Y+ +F +               ++ +L      L  ++
Sbjct: 316 ILEFIDRGCNEFVSNLCLVIASYQELFINRPQESELASKNIPEMANGKLHVFVDTLAARY 375

Query: 301 FDATEQFIKKQICAAD---LLRVFGIIWIDVLLLGEVLNDAGLP----DYSLKAAQVAVK 360
           F   E+ I+++    D   L+R        +  + ++L  + +P    +  ++AA+  +K
Sbjct: 376 FSLVERRIQEEKGVGDNSLLVRALDRFHRRLQAISKLLPGSAVPSQGTEIVVRAARERIK 435

Query: 361 QYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQG--SMDVLLDFRQ 420
           QY+    S L     D+LT V  R+       S+  A  +   AL+ G  S D      +
Sbjct: 436 QYL----SALQTFYHDSLTDV--RQALAAPRLSVGGASASGGGALVGGASSKDAPPSLPE 495

Query: 421 LLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLTEATQAEKV 480
           LL   S  I+N                 K+++A   L + K+ +FS          ++ V
Sbjct: 496 LLTSLSNFILNQ---------------LKSVLASVHLFTAKDITFSNKPYFKGEFCSQGV 555

Query: 481 IAGLVLEIAASF--------SGGGIRGYEYGPAFV------------------------- 540
             GLV+                 G RG    PA +                         
Sbjct: 556 REGLVVSFIKFICQSSRQYCESAGDRGGSTPPALLLLLSRLCLDYETSTISYILTLTDEQ 615

Query: 541 -----------PAEICRMFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREV 600
                         +C   R A +KLL+ Y+ ++   IS +L K   T +WV   EPR V
Sbjct: 616 FLVQHHTPVTPVTALCAEAREAAQKLLNHYVKVQGLIISQMLRKSVETRDWVNTIEPRNV 675

Query: 601 HMFVDLFLQELEAVGSEVKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRAR 660
              +   +++  ++  +V  +  +G RK   +DS+  T S  S+  ++ +   S T  A 
Sbjct: 676 RAVMKRVVEDTTSIDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRQQIRYAPSYTPSAP 735

Query: 661 SQL-LETHLAKLFKQKIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQ 674
               L +++ KLF ++I+IF+ VEF + SVLT  +K+SLKT  E VRL+TF R G QQIQ
Sbjct: 736 MDTNLLSNIHKLFSERIDIFSPVEFNKVSVLTGIIKISLKTFLECVRLRTFGRYGLQQIQ 795

BLAST of ClCG10G006350 vs. ExPASy Swiss-Prot
Match: Q4V9Y0 (Vacuolar protein sorting-associated protein 51 homolog OS=Xenopus tropicalis OX=8364 GN=vps51 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.3e-54
Identity = 191/705 (27.09%), Postives = 332/705 (47.09%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           M  +I++LD+++Q LVYENYNKFISATDTI++M N+   ME  M+ L   +  +   S  
Sbjct: 74  MVRQIRSLDSEMQTLVYENYNKFISATDTIRKMKNDFKKMEDEMDGLATNMAVITEFSAR 133

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           ++++L E+ + I KL     LLRK+QF+++LPARL KCI+  AYA AV +++ A  +   
Sbjct: 134 ISSTLQERHQQITKLSGVHTLLRKLQFLFELPARLKKCIELGAYAQAVSYHSKARSVLHQ 193

Query: 121 YGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKL 180
           Y    SF   +   +  +A +   L+++    + S Q  +E   +L  L+ P   L  + 
Sbjct: 194 YQHMPSFHGIQTDCQAIMAGLADTLRQRFRDPASSPQDLSECVEMLLNLEEPAHLLCDEF 253

Query: 181 LE----KLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYR 240
           L     +L     DLQ + + L  E V            +  AS+++   + A +     
Sbjct: 254 LAHGRGRLASHLSDLQESGDIL--EFVDRGCGGFISDACLLAASYQSLFSKEAGSTAQM- 313

Query: 241 VIFSDSDRQLIKLAQDLITKHFDATEQFIKKQICAAD---LLRVFGIIWIDVLLLGEVLN 300
                ++ +L    ++L T +F+  E+ ++++    D   L+R        +    +++ 
Sbjct: 314 -----AEAKLTSFLEELSTGYFELVEKRLRQEKSLGDNSLLVRALDRFHRRLQAPSKLVP 373

Query: 301 DAGL----PDYSLKAAQVAVKQYVTFT---FSRLLQDISDALTQVHTRKKEGVQEYSLQL 360
             G      +  ++AAQ  + QY+      F   L D+  AL       KE      L  
Sbjct: 374 GCGFNRRGTEIVVRAAQERLAQYLQALKDFFQGCLTDVRQALAAPRLPGKEMPALGDLLA 433

Query: 361 ALEAS-----KKAL----LQGSMDVLLDFRQLL-------GDQSGLIINLRDSIVDWVQE 420
            L AS     K  L    L  + DV    +          G + GLI+    S+    ++
Sbjct: 434 GLSASVLNQIKTVLAAVHLFTAKDVAFSDKPYFKGEFCSQGVREGLIVAFIKSVCQTARQ 493

Query: 421 GFQDFFKALVARFMLLSGKNNSFSESQVLTEATQAEKVIAGLVLEIAASFSGGGIRGYEY 480
                       F  + G+  + +   +L   ++         +    + +     G ++
Sbjct: 494 ------------FCEIPGEKGTSTPPALLLLLSRLCLDYETSTISYILTLTDEQFLGQDH 553

Query: 481 GPAFVPAEICRMFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDL 540
            P    + +C + R+  + LL+ Y+  +   +S +L K   T +WV   EPR V   +  
Sbjct: 554 SPVTPVSSLCSLARSTAQTLLNQYVKSQGLVVSQMLRKSVETRDWVTTIEPRNVRAVMKR 613

Query: 541 FLQELEAVGSEVKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQL-LE 600
            ++++  V  +V  +  +G RK   +DS+  T S  S+   + +  +S T  A     L 
Sbjct: 614 VVEDITGVDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRLQGRYAQSYTPSAPMDTNLL 673

Query: 601 THLAKLFKQKIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFL 660
           +++ KLF ++I+IF+ V+F + S+LT  +K+SLKT  E VRL+TF R G QQIQ+D  +L
Sbjct: 674 SNIQKLFSERIDIFSTVQFNKVSILTGIIKISLKTFLECVRLRTFGRYGLQQIQVDCHYL 733

Query: 661 RTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQ 674
           +  L     DE  +  LLDEV+ +A+ RCLDP P+E  +++ + +
Sbjct: 734 QLYLWRFVSDENLVHCLLDEVVGSAAHRCLDPAPMEQSVIEVICE 758

BLAST of ClCG10G006350 vs. ExPASy TrEMBL
Match: A0A6J1EMM4 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 647/699 (92.56%), Postives = 661/699 (94.56%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFK 
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKV 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAEDLSS LVIGSSKDGN SE VYGASHEASVREFAEA+RAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASVREFAEAIRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDATEQFI+KQICAADLLRVFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSLQLALEASKKALLQGSM 360
           LKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSLQLALEASKKA+LQGSM
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSM 436

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           DVLLDFRQLL DQSGL I+ RDSIVDWVQEGFQDFF+ALV RFMLLSGKN+SFS+SQVL 
Sbjct: 437 DVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLP 496

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           EATQAEKV+AGLVL                   EIAASFSGGG RGYEYGPAFVPAEICR
Sbjct: 497 EATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGTRGYEYGPAFVPAEICR 556

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           MFRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE
Sbjct: 557 MFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 616

Query: 541 VKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIE 600
           VKQ+LPQGTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIE
Sbjct: 617 VKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIE 676

Query: 601 IFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEA 660
           IFTRVEFTQGSV+TTAVKLSLKTL EFVRLQTFNRSGFQQ+QLDMQFLRTPLKEIADDEA
Sbjct: 677 IFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEA 736

Query: 661 AIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           AIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 AIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKA 775

BLAST of ClCG10G006350 vs. ExPASy TrEMBL
Match: A0A1S3CES5 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucumis melo OX=3656 GN=LOC103500085 PE=3 SV=1)

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 643/698 (92.12%), Postives = 659/698 (94.41%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLK KLL
Sbjct: 197 YGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKVKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAE+L+S LV  SSKDGN SELVYGASHEASVREF EAVRAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASVREFTEAVRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFD+TEQFIKKQICAADLL VFG IW DVLLLGEVLNDAGL DYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDVLLLGEVLNDAGLLDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSMD 360
           LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQL LEASKKA+LQGSMD
Sbjct: 377 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLELEASKKAVLQGSMD 436

Query: 361 VLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLTE 420
           +LLDFRQLL DQSGLIIN RDSIVDWVQEGFQDFF+ALV RFMLLSGKNNS+++SQ LTE
Sbjct: 437 ILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNNSYTQSQALTE 496

Query: 421 ATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICRM 480
           ATQAEKV AGLVL                   EIAASFSGGGIRGYEYGPAFVPAEICRM
Sbjct: 497 ATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFVPAEICRM 556

Query: 481 FRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 540
           FRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV
Sbjct: 557 FRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 616

Query: 541 KQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIEI 600
           KQ+LP+GTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIEI
Sbjct: 617 KQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEI 676

Query: 601 FTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA 660
           FTRVEFTQGSV+TT VKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA
Sbjct: 677 FTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA 736

Query: 661 IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA
Sbjct: 737 IDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKA 774

BLAST of ClCG10G006350 vs. ExPASy TrEMBL
Match: A0A6J1JAN2 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 641/699 (91.70%), Postives = 659/699 (94.28%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFK 
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKV 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNAEDLSS LVIGSSKDGN SE VYGASHE SVREFAEA+RAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSVREFAEAIRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHF+A EQFIKKQICAADLLRVFGIIW DVLLLGEVLNDAGLPDYS
Sbjct: 317 SDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSLQLALEASKKALLQGSM 360
           LKAAQVAVKQYVT  FSRLLQDISDAL QVHTRKK EGVQEYSLQLALEASKKA+LQGSM
Sbjct: 377 LKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSM 436

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           DVLLDFRQLL DQSGL I+ RDSIVDWVQEGFQDFF+ALV RFMLLSGKN+S+S+SQVL 
Sbjct: 437 DVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSYSQSQVLP 496

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           EATQAEKV+AGLVL                   EIAASFSGGG RGYEYGPAFVPAEICR
Sbjct: 497 EATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGTRGYEYGPAFVPAEICR 556

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           MFRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEA+GSE
Sbjct: 557 MFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAIGSE 616

Query: 541 VKQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIE 600
           VKQ+LPQGTRKHRR DSNGSTTSSRSNPLREEKLN+SNTQRARSQLLETHLAKLFKQKIE
Sbjct: 617 VKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIE 676

Query: 601 IFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEA 660
           IFTRVEFTQGSV+TTAVKLSLKTL EF+RLQTFNRSGFQQ+QLDMQFLRTPLKEIADDEA
Sbjct: 677 IFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEA 736

Query: 661 AIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           AIDFLLDEVIVAASERCLD IPLEPPILDKLI+AKLAKA
Sbjct: 737 AIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKA 775

BLAST of ClCG10G006350 vs. ExPASy TrEMBL
Match: A0A6J1GBC0 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111452610 PE=3 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 630/698 (90.26%), Postives = 653/698 (93.55%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLK LQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNA+DLSS L+I S K+ N SE  YGA+HEASVREFAEAVRAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGAAHEASVREFAEAVRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SD QLIKLAQDL+TKHFDA EQFIKKQI AADLLRVFGIIW DVLLLGEVLNDA L DYS
Sbjct: 317 SDMQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDVLLLGEVLNDACLHDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSMD 360
           LKAAQVAVKQYVT TFSRLLQ+ISDALT+VHTRKKE VQEYSLQLALEA KKA+LQGSMD
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQLALEAGKKAVLQGSMD 436

Query: 361 VLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLTE 420
           VLLDFR LL +QSGLIIN RDSIVDWVQEGFQDFF+ALV RFMLLSGKNNS+S+SQVLTE
Sbjct: 437 VLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNNSYSQSQVLTE 496

Query: 421 ATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICRM 480
           ATQA+KV+AGLVL                   EIAASFSGGG+RGYEYGPAFVPAEICRM
Sbjct: 497 ATQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVRGYEYGPAFVPAEICRM 556

Query: 481 FRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 540
           FRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV
Sbjct: 557 FRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 616

Query: 541 KQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIEI 600
           +Q+L QGTRKHRR +SNGSTTSSRS PLREEKLNK+N QRARSQLLE+HLAKLFKQKIEI
Sbjct: 617 RQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNKTNAQRARSQLLESHLAKLFKQKIEI 676

Query: 601 FTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA 660
           FTRVEFTQGSV+TTAVKLSLKTLQEFVRLQT+NRSGFQQIQLDMQFLRTPLKEIADDEAA
Sbjct: 677 FTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDMQFLRTPLKEIADDEAA 736

Query: 661 IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA
Sbjct: 737 IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 774

BLAST of ClCG10G006350 vs. ExPASy TrEMBL
Match: A0A6J1K9H7 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111492889 PE=3 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 629/698 (90.11%), Postives = 652/698 (93.41%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG
Sbjct: 77  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 136

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA
Sbjct: 137 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 196

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGDSSFQDCKRASEEAIAIVLK LQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL
Sbjct: 197 YGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 256

Query: 181 EKLEQSTLDLQLNAEDLSSELVIGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFSD 240
           EKLEQSTLDLQLNA+DLSS L+I S K+ N SE  YGASHEASVREFAEAVRAYRVIF+D
Sbjct: 257 EKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGASHEASVREFAEAVRAYRVIFAD 316

Query: 241 SDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDYS 300
           SDRQLIKLAQDL+TKHFDA EQFIKKQI AADLLRVFGIIW DVLLLGEVLNDA L DYS
Sbjct: 317 SDRQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDVLLLGEVLNDACLHDYS 376

Query: 301 LKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSMD 360
           LKAAQVAVKQYVT TFSRLLQ+ISDALT+VHTRKKE VQEYSLQLALEA KKA+LQGSMD
Sbjct: 377 LKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQLALEAGKKAVLQGSMD 436

Query: 361 VLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLTE 420
           VLLDFR LL +QSGLIIN RDSIVDWVQEGFQDFF+ LV RFMLLSGKNNS+S+SQVLTE
Sbjct: 437 VLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRELVDRFMLLSGKNNSYSQSQVLTE 496

Query: 421 ATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICRM 480
           A QA+KV+AGLVL                   EIAASFSGGG+RGYEYGPAFVPAEICRM
Sbjct: 497 ANQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVRGYEYGPAFVPAEICRM 556

Query: 481 FRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 540
           FRAAGEK LHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV
Sbjct: 557 FRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEV 616

Query: 541 KQVLPQGTRKHRRADSNGSTTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQKIEI 600
           +Q+L QGTRKHRR +SNGSTTSSRS PLREEKLN+S+ QRARSQLLE+HLAKLFKQKIEI
Sbjct: 617 RQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNRSSAQRARSQLLESHLAKLFKQKIEI 676

Query: 601 FTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAA 660
           FTRVEFTQGSV+TTAVKLSLKTLQEFVRLQT+NRSGFQQIQLDMQFLRTPLKEIADDEAA
Sbjct: 677 FTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDMQFLRTPLKEIADDEAA 736

Query: 661 IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA
Sbjct: 737 IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 774

BLAST of ClCG10G006350 vs. TAIR 10
Match: AT4G02030.1 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 865.1 bits (2234), Expect = 3.9e-251
Identity = 468/702 (66.67%), Postives = 558/702 (79.49%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDG
Sbjct: 73  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDG 132

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI K 
Sbjct: 133 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKV 192

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLKAKLL
Sbjct: 193 YGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLL 252

Query: 181 EKLEQSTLDLQLNAEDLSSELV-IGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFS 240
           EKLEQS   LQ+  E+ S+ +    SS D   ++      HE +VR F+EA+RAYR IF 
Sbjct: 253 EKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFP 312

Query: 241 DSDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDY 300
           DS+ +L KLA+ L   HF+  E +IKK++ AAD L +F I+W DV+L+ EVL +A L D 
Sbjct: 313 DSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDL 372

Query: 301 SLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSM 360
           S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L++ LEAS+KA+LQG+ 
Sbjct: 373 SAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTT 432

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           ++  DFRQLL +++G+ I ++D I  W+Q+G QDFF++L A+F++LSGK +S ++     
Sbjct: 433 NIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSND----I 492

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           E   ++K+ AGL+L                   EIAASFSGG  + +E GPAF+P E+CR
Sbjct: 493 EGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCR 552

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           +F AA EKLL  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVHM+VD+FL ELE VG E
Sbjct: 553 VFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKE 612

Query: 541 VKQVLPQGT-RKHRRADSNGS--TTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQ 600
           VKQVLPQGT RKH+R DSNGS  TTSSRSN L  +K+ +SN+QRARSQL ETHLAKLFKQ
Sbjct: 613 VKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQ 672

Query: 601 KIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIAD 660
           K+EIFT+VEFTQ SV+TT VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+ PLKE  +
Sbjct: 673 KVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVE 732

Query: 661 DEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+
Sbjct: 733 DEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKS 770

BLAST of ClCG10G006350 vs. TAIR 10
Match: AT4G02030.2 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 865.1 bits (2234), Expect = 3.9e-251
Identity = 468/702 (66.67%), Postives = 558/702 (79.49%), Query Frame = 0

Query: 1   MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDG 60
           MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDG
Sbjct: 98  MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDG 157

Query: 61  VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKA 120
           VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI K 
Sbjct: 158 VNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKV 217

Query: 121 YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLL 180
           YGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLKAKLL
Sbjct: 218 YGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLL 277

Query: 181 EKLEQSTLDLQLNAEDLSSELV-IGSSKDGNGSELVYGASHEASVREFAEAVRAYRVIFS 240
           EKLEQS   LQ+  E+ S+ +    SS D   ++      HE +VR F+EA+RAYR IF 
Sbjct: 278 EKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFP 337

Query: 241 DSDRQLIKLAQDLITKHFDATEQFIKKQICAADLLRVFGIIWIDVLLLGEVLNDAGLPDY 300
           DS+ +L KLA+ L   HF+  E +IKK++ AAD L +F I+W DV+L+ EVL +A L D 
Sbjct: 338 DSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDL 397

Query: 301 SLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKALLQGSM 360
           S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L++ LEAS+KA+LQG+ 
Sbjct: 398 SAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTT 457

Query: 361 DVLLDFRQLLGDQSGLIINLRDSIVDWVQEGFQDFFKALVARFMLLSGKNNSFSESQVLT 420
           ++  DFRQLL +++G+ I ++D I  W+Q+G QDFF++L A+F++LSGK +S ++     
Sbjct: 458 NIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSND----I 517

Query: 421 EATQAEKVIAGLVL-------------------EIAASFSGGGIRGYEYGPAFVPAEICR 480
           E   ++K+ AGL+L                   EIAASFSGG  + +E GPAF+P E+CR
Sbjct: 518 EGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCR 577

Query: 481 MFRAAGEKLLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSE 540
           +F AA EKLL  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVHM+VD+FL ELE VG E
Sbjct: 578 VFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKE 637

Query: 541 VKQVLPQGT-RKHRRADSNGS--TTSSRSNPLREEKLNKSNTQRARSQLLETHLAKLFKQ 600
           VKQVLPQGT RKH+R DSNGS  TTSSRSN L  +K+ +SN+QRARSQL ETHLAKLFKQ
Sbjct: 638 VKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQ 697

Query: 601 KIEIFTRVEFTQGSVLTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIAD 660
           K+EIFT+VEFTQ SV+TT VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+ PLKE  +
Sbjct: 698 KVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVE 757

Query: 661 DEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKA 680
           DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+
Sbjct: 758 DEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKS 795