ClCG10G003210 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG10G003210
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionmethylsterol monooxygenase 1-1-like
LocationCG_Chr10: 3969376 .. 3973898 (-)
RNA-Seq ExpressionClCG10G003210
SyntenyClCG10G003210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCTCTTCCTCTTCCTCTTCCCTTCTTCCTTTTCAAACTTCCCGCATTAATTTAACTGTCTCTCACCCTCTCCTTCTTTCATTTCACCCCTTTTCGCCGCTTCTCAATCCATTCACCGCCGGCGAACTTCTCTCCACCTCTAATCCCCATCTGGGTTCCTCTCCGCCGGCGGATTCCTCCACCCAATGTTGCCCTTTCACTCCATCTCCGCCGCTCAGGCCGCTCTTGGCCGGAATCTCACCTTCTTTGAAACTCTCTGGTTCAATTACTCCCATGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTCTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTCATCTTCCTTGAGCTCTTGCATGCCTCTGGGATTCATAAATACAAAATTCAGCCCAAGGTCCGATTGCCATTCTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGTATGTTCTTCCTTGTCGTTGGCCCTCTTCAGCTCGTCTCATTCCCTTCCATTCAGGTGAATCTCGGATTTCCATTTTCTTCGATTTGCTCCCTCTTTTTTGATCTGGGATTTCGATTTTGATTTTTTTTTTCCCTGTTTCTATTGGGTTATTAGATGATTGGGATCAGGACGGGTTTGCCATTGCCTTCTGGGTGGGAAATTCTTTCCCAATTGGTGGTGTATTTCATGGTGGAAGATTATACCAATTATTGGATGCATAGATTCCTCCATGGCAAATGGGGTTATGAGAAAATCCATCGTGTTCACCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGATCCTTGGAATTCCTTCTTTTCTTGGTCCAGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTATGGATTGCTTTAAGGCAGATTGAGGCCATTGACACACATAGTGGGTATGAATTGCTAAAGCTATTTCTCTTCCTCTCTGTCTCTCACTCTGTGTCTAGCTTTCTTGCTTTCTATTTTAATAAATTTTTCTTCCCCTTTCTTTTCACTTCTGCACTGTGCTTTTATTCGACTGCGACAACATGATGCCGACTTTGTATTTTCTGCATTCTTTTATCTCTTCACCAATGCCTTTGTGTTAAAGTACTTTTAATCTGAAGGATCTTGAATGGTGGGCTTGCCTCTTTTTTGACTAGGACCGTGATCTGCTCTATGGCTTTCATCCTTTTTAATCTTAGTTGCCTTTTCCTTATTATATATATATTTTCTATATGTGTGGATATATGTTTAGTCACAATATAGAGTTCCTATCATTACTGTATGAACTTATTGCATGCTGAATCATTGCTCCTACTAACTTGATATAATGAAGGTTTGGAGTTTTTATTGCGAAATATTGAAAAGGTTAAATTTACCCTTTTTTCTAGCATCAACTTTCTCAGTTTCCCCATTCTTCTTCATACAGTTGTGGGAAATGTCAAGATTCCTTTGAGCTTATTCTAAGAGGATGTATAAATCTTCTATGAGAAGCTCAGCCTAAGAAGCTTGATTCTTATATACTAAACTAATTGGGGCCCCACTACCCCTTTTATGCTTTAAACTTATAAACACCATTTCAATATCTATGATTTAACATGCTATGAAAGTAGCAAGTCTTGAATTGCAGAGCAATGTTATTGTTATTATTTGTTTTTGTTTTTGTAATTTTGCATTTATGACATCTGTATTAGGCTTTGTGCCAACACTTGACTCACGTAAAGGGAACTAGGAGTACTGATAATTTAAGAAAAGTAAATATTGAATTTAGGCTAACCGATCAAAGTTCAATAGTGGACAACAGCATTTGACTTTACTTTCTCTGATTAGTGGGACCATAGATGATAGATAAATCTTAGGCTATAGGTTTTAAAGAAGAATGAAATTAGCGTATGAGTGAAGGAAAAACAGAAAAGTGGTTGTGTAAACGTATTTGTGTCGATGGTAGGTAAGTACAATGAAACGAATGGGGAATGTTGTTTTGAAGTGATGCTGCAATGATATTGATGGGCGAAGATCATGAACTAGATCATATCTTAACCTTATTGGTTTAAACATCCTTTGCTTCAGGGGGACCTTTAGCTCACAACCATTCCCAATCTTCTTGATCTTACAACATTGTAGTTGTTTATGAGGGTTTAGGTTTGGGGTTGTTGGGAAAAGAAAGGTAAAAAGAAATCTTAATAGAATTGCTTCTAGCACGATGGAATAGACATGTAGGAGCACTCATGGGTTAGAGTCGTGTAACCTGGGGTTAAGAAGCCGAACATATTCTAGCATATGGTTTGCTTATCTTGAGCTATGAGTTCGCTAGTTTCATGGGCTACATAAACCCACTAGTGATACAAATCCAAGTTTCCATAACCTATTCCTCGGTACAGTCTAAACACAAATTAACTCTATAAACTCAGCTTTTCAATAAACCAACCTTATTAAATTGTACTGTTCCTCTTCCTCCTCAGAATAATCAAAAGCAAGCTCTGTTATTCATGTTGACTGCATTTGTTTTAGAACTGTTATTAAATTTAGAGGAGTGAAGAGGGAAAGAGAAGTAAATTGACATCTCAAGGGGCCAGCTTACTCAAATAAGAGAATCCATTGATTTGACTTCCAAATTGTTTAATTGGGCCAGAAATGAAAAATAATTGATGCCAGGCTTATGGCTGGGAATAAATGCGGTGTCAGACAATCATTTCTTTGGTCATGTAGGACATAGCTTCACTCTTGGTGGGTTTCCTGTGGCTGAACATCCCCTTAGCCTTTTTGTTGAACTTGAATAACTTTTATATATTATTATTTTAGATTGCTGAAGTTTGAATGTTGTCTCTTTCATACAAAGACTGAGATTCGCATGAACATTGATGGCAAGATGTTGTACAACTTTTGATGCAAACAAGAATGCCAAAAAATAAAATAAAATAAAATAAAATATAAAAATTGAAGTCTTCTATTCATCACAGTAATCTGAAGTTGAAGGATCTTTTTTTTATTTTTTTATTTTTTAAATCAATATATTTCACAAGCATTAGTGTCAATGAACTATCGCCTCTTCATTCGTAATTGATTTATCGATGTTAGTTTAAATTGGCATTATAATTCTTATATTTTGAGCTTGATTTCATGTTTTCTAATGGACGTTATAGAGAGGAGTGACGAGTTGACATTTAGTATAAAGCATGTGTGAAGCTTATAAGAGGATAAGCCTTACTGCGTAACTTCAATGATCCTAATCTCCAAAATCCTCTCTACTGCGTCCATGGAAACTTACTAGTAGAGACGTTTTGGGAGGTATAACCAAACTATAGGGACTTCAATAAACTTTTGAGGACAAATTGAAACCGAGCACAAACTATAGGGAGCAAAATGGTAATATGACCTTATTTTTGTTACAAAAACTATTCTTGAGAATACATCATTTCTCTTGTTCTTTAGTGTGGACTTCTTTTACCTCTGCTTTTCTCTCAGATTGTGCTTTTATGATGATTGTTGAGATCATCCATGGTTTCATTCATATATTTTATATTTATATTCAGGTATGATTTCCCTTGGAGTCTAACAAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATGGTCTTGCTATAATTTTACCTCCCACTTATTCTTCCAGTAAAATCATGCTCATAAAAGTTACTCATTTGTTTACTTCCTCAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCGTTTCGTTTTTCCCCCGTCCCCACCTTCGCCCCAAAAGTTAGTCATATATGGTGTCATGGATTTGCATTAGTCTCAAGTTTTCTTCAACTGGACATTAGATTTAACCGAAAGTGAGCTTGGTTCGATGGCGTTGCCAATAAGCTTGATCAAGCCTTAGGATGGCTAGCTTGGCAATATTTACTATGCCTTGCTCCGGTTCTATTTGGTTGGATCAAATCTATGCCATTTGTTCTTTTGGTCCTCAGAACCTGTTCCATTTTTTATTTTGCTTTGTTTTGGAGGATGGGGTTTTGATTGTGAATAAGAAAGAAAAAGAAAGGACAATTTATGACTGCTAATGTTTTTCTCATGTTCGTATCGATACAATGCTACTATGTAGTTGGTGATATATCGCATGTTCTAATTGTTTGTTGTTGGTGTGATCAGTTGAAGGAAGAGGCCAAGAACAGTGAAGAGTCATACTACAGCACAGCCCAAGATGTTAAATCGGACTAGATGATGAAAAGGGGATTCGTGGGATTTTGTCGTGTCAGTAGCAGCGTTACGTGATCTCTGATTTGAAGTTACTTGGTCGGGATCAGATCATCAACGAGATAGCTAGGGACTGGGATGGGATGGGATGGAATGGGATGGAATGGAAGTTAACAAGGGGTCTATAAAGTGTTTTTTTTTTCTTTTTTTTTT

mRNA sequence

CTTCCTCTTCCTCTTCCTCTTCCCTTCTTCCTTTTCAAACTTCCCGCATTAATTTAACTGTCTCTCACCCTCTCCTTCTTTCATTTCACCCCTTTTCGCCGCTTCTCAATCCATTCACCGCCGGCGAACTTCTCTCCACCTCTAATCCCCATCTGGGTTCCTCTCCGCCGGCGGATTCCTCCACCCAATGTTGCCCTTTCACTCCATCTCCGCCGCTCAGGCCGCTCTTGGCCGGAATCTCACCTTCTTTGAAACTCTCTGGTTCAATTACTCCCATGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTCTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTCATCTTCCTTGAGCTCTTGCATGCCTCTGGGATTCATAAATACAAAATTCAGCCCAAGGTCCGATTGCCATTCTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGTATGTTCTTCCTTGTCGTTGGCCCTCTTCAGCTCGTCTCATTCCCTTCCATTCAGATGATTGGGATCAGGACGGGTTTGCCATTGCCTTCTGGGTGGGAAATTCTTTCCCAATTGGTGGTGTATTTCATGGTGGAAGATTATACCAATTATTGGATGCATAGATTCCTCCATGGCAAATGGGGTTATGAGAAAATCCATCGTGTTCACCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGATCCTTGGAATTCCTTCTTTTCTTGGTCCAGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTATGGATTGCTTTAAGGCAGATTGAGGCCATTGACACACATAGTGGGTATGATTTCCCTTGGAGTCTAACAAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGTTGAAGGAAGAGGCCAAGAACAGTGAAGAGTCATACTACAGCACAGCCCAAGATGTTAAATCGGACTAGATGATGAAAAGGGGATTCGTGGGATTTTGTCGTGTCAGTAGCAGCGTTACGTGATCTCTGATTTGAAGTTACTTGGTCGGGATCAGATCATCAACGAGATAGCTAGGGACTGGGATGGGATGGGATGGAATGGGATGGAATGGAAGTTAACAAGGGGTCTATAAAGTGTTTTTTTTTTCTTTTTTTTTT

Coding sequence (CDS)

ATGTTGCCCTTTCACTCCATCTCCGCCGCTCAGGCCGCTCTTGGCCGGAATCTCACCTTCTTTGAAACTCTCTGGTTCAATTACTCCCATGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTCTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTCATCTTCCTTGAGCTCTTGCATGCCTCTGGGATTCATAAATACAAAATTCAGCCCAAGGTCCGATTGCCATTCTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGTATGTTCTTCCTTGTCGTTGGCCCTCTTCAGCTCGTCTCATTCCCTTCCATTCAGATGATTGGGATCAGGACGGGTTTGCCATTGCCTTCTGGGTGGGAAATTCTTTCCCAATTGGTGGTGTATTTCATGGTGGAAGATTATACCAATTATTGGATGCATAGATTCCTCCATGGCAAATGGGGTTATGAGAAAATCCATCGTGTTCACCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGATCCTTGGAATTCCTTCTTTTCTTGGTCCAGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTATGGATTGCTTTAAGGCAGATTGAGGCCATTGACACACATAGTGGGTATGATTTCCCTTGGAGTCTAACAAAATTTATACCATTTTATGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGGCAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGTTGAAGGAAGAGGCCAAGAACAGTGAAGAGTCATACTACAGCACAGCCCAAGATGTTAAATCGGACTAG

Protein sequence

MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLELLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPLPSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD
Homology
BLAST of ClCG10G003210 vs. NCBI nr
Match: XP_038876881.1 (methylsterol monooxygenase 1-2-like [Benincasa hispida])

HSP 1 Score: 602.4 bits (1552), Expect = 2.1e-168
Identity = 289/300 (96.33%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL
Sbjct: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH KWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIISQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILK+LKEE KN E  Y+STAQDVKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKQLKEEVKNIEGPYHSTAQDVKSD 300

BLAST of ClCG10G003210 vs. NCBI nr
Match: XP_023552163.1 (methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 601.3 bits (1549), Expect = 4.6e-168
Identity = 287/300 (95.67%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQ ALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           +LHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH KWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEE K+SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of ClCG10G003210 vs. NCBI nr
Match: XP_022965134.1 (methylsterol monooxygenase 1-2 [Cucurbita maxima])

HSP 1 Score: 600.5 bits (1547), Expect = 7.9e-168
Identity = 287/300 (95.67%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSISAAQ ALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           +LHASGIHK+KIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH KWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEE K+SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of ClCG10G003210 vs. NCBI nr
Match: XP_022923513.1 (methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol monooxygenase 1-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015639.1 Methylsterol monooxygenase 1-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.1 bits (1546), Expect = 1.0e-167
Identity = 286/300 (95.33%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQ ALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           +LHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH KWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGG+DHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEE K+SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of ClCG10G003210 vs. NCBI nr
Match: XP_011650426.1 (methylsterol monooxygenase 1-2 [Cucumis sativus] >KGN55922.1 hypothetical protein Csa_009711 [Cucumis sativus])

HSP 1 Score: 596.3 bits (1536), Expect = 1.5e-166
Identity = 285/300 (95.00%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQA+LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           LLHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EI+SQLVVYFMVEDYTNYW+HRFLH KWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEE KNSEESYY+TAQ+VKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300

BLAST of ClCG10G003210 vs. ExPASy Swiss-Prot
Match: Q1EC69 (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 9.5e-132
Identity = 217/290 (74.83%), Postives = 258/290 (88.97%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ +I  A  AL RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  LLHASG--IHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
              ++    ++YKIQPKV+  FS +F+CYKDVM+MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLPS  EI++QLVVYF+VEDYTNYW+HRF H KWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE++K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKSNK 290

BLAST of ClCG10G003210 vs. ExPASy Swiss-Prot
Match: Q8L7W5 (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.8e-131
Identity = 217/290 (74.83%), Postives = 259/290 (89.31%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  ALGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  LLH-ASGI-HKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
           L   ASG+ ++YKIQPKV    S++F+CYKDVM MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLP+  E+LSQLVVYF++EDYTNYW+HRF H KWGY+KIHRVHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE +K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNK 290

BLAST of ClCG10G003210 vs. ExPASy Swiss-Prot
Match: Q9AV39 (Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-10 PE=2 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.2e-119
Identity = 197/280 (70.36%), Postives = 238/280 (85.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ + + A+AALGR +T  E+LWF YS    DY LF HNILFLF++F+  PLP+  LE
Sbjct: 1   MLPYATAAEAEAALGRAMTAAESLWFRYSAGIPDYVLFWHNILFLFVVFTLAPLPVALLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           L   + +  +K+QPKVRL   E FRCY+DVMR+FFLV+GPLQLVS+P+++M+GI TGLPL
Sbjct: 61  LRAPAAVGPFKLQPKVRLSREEFFRCYRDVMRLFFLVIGPLQLVSYPTVKMVGIHTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  E+ +QL+VYF+VEDY NYW+HR LHG+WGYEKIHRVHHE+TAPIGFAAPYAHWAEV
Sbjct: 121 PSLGEMAAQLLVYFLVEDYLNYWIHRLLHGEWGYEKIHRVHHEFTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSF+GPA+ PGHMITFWLWI LRQ+EAI+THSG+DFP++LTK+IPFYGGA++HDY
Sbjct: 181 LILGIPSFVGPALAPGHMITFWLWIVLRQMEAIETHSGFDFPFNLTKYIPFYGGAEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLK 281
           HHYVG QS SNFASVFTYCDY+YGTDKGYRY K    K+K
Sbjct: 241 HHYVGRQSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMK 280

BLAST of ClCG10G003210 vs. ExPASy Swiss-Prot
Match: F4JLZ6 (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.6e-118
Identity = 193/285 (67.72%), Postives = 245/285 (85.96%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
                   ++KIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EI++QLVVYF++EDYTNYW+HR++H KWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-KKLKEEAK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEEA+
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of ClCG10G003210 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.4e-57
Identity = 117/264 (44.32%), Postives = 158/264 (59.85%), Query Frame = 0

Query: 19  TFFETLWFNYSHDKSDYFLFCHNILFLF-LIFSFIPLPLIFLELLHASGIHKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPLPSGWEILSQLVVYFMVE 138
            P ++  +C   ++   F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++E
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFT 258
           +IT WLW+ LR +E ++ H GY FPWSL+ F+P YGGAD HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKILKKLKE 282
           Y D+I+GTDKGYR     LK LKE
Sbjct: 243 YMDWIFGTDKGYRR----LKTLKE 259

BLAST of ClCG10G003210 vs. ExPASy TrEMBL
Match: A0A6J1HQ57 (methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 3.8e-168
Identity = 287/300 (95.67%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSISAAQ ALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           +LHASGIHK+KIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH KWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEE K+SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of ClCG10G003210 vs. ExPASy TrEMBL
Match: A0A6J1E9U9 (methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.0e-168
Identity = 286/300 (95.33%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQ ALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           +LHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH KWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGG+DHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEE K+SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of ClCG10G003210 vs. ExPASy TrEMBL
Match: A0A0A0L5P8 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G036480 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.2e-167
Identity = 285/300 (95.00%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQA+LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           LLHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EI+SQLVVYFMVEDYTNYW+HRFLH KWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEE KNSEESYY+TAQ+VKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300

BLAST of ClCG10G003210 vs. ExPASy TrEMBL
Match: A0A1S3BKU6 (methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.0e-165
Identity = 286/301 (95.02%), Postives = 296/301 (98.34%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQA+LGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           LLHASGI KYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+MIGIRTGLPL
Sbjct: 61  LLHASGIRKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EI+SQLVVYFMVEDYTNYW+HRFLH KWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEE-SYYSTAQDVKS 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+KLKEE KNSEE SYY+TAQ+VKS
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESSYYNTAQNVKS 300

BLAST of ClCG10G003210 vs. ExPASy TrEMBL
Match: A0A6J1FUF2 (methylsterol monooxygenase 1-1-like OS=Cucurbita moschata OX=3662 GN=LOC111448229 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 280/300 (93.33%), Postives = 289/300 (96.33%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHS+SAAQAALGRNLT FETLWFNYSHDKS YFL+CHNILFLFLIFS IPLPLIFLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTSFETLWFNYSHDKSSYFLYCHNILFLFLIFSLIPLPLIFLE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           L H SGIHKYKIQPKVRLP +EIFRCYKDVMRMFFLVVGPLQLVS+PSI MIGIRTGLPL
Sbjct: 61  LFHVSGIHKYKIQPKVRLPSAEIFRCYKDVMRMFFLVVGPLQLVSYPSINMIGIRTGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYW+HRFLH +WGYEKIHRVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWVHRFLHCEWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWS+TK IPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSVTKLIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEESYYSTAQDVKSD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAK SE SYYSTAQDVKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKISEASYYSTAQDVKSD 300

BLAST of ClCG10G003210 vs. TAIR 10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2 )

HSP 1 Score: 471.1 bits (1211), Expect = 6.7e-133
Identity = 217/290 (74.83%), Postives = 258/290 (88.97%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ +I  A  AL RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  LLHASG--IHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
              ++    ++YKIQPKV+  FS +F+CYKDVM+MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLPS  EI++QLVVYF+VEDYTNYW+HRF H KWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE++K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKSNK 290

BLAST of ClCG10G003210 vs. TAIR 10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1 )

HSP 1 Score: 469.5 bits (1207), Expect = 2.0e-132
Identity = 217/290 (74.83%), Postives = 259/290 (89.31%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  ALGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  LLH-ASGI-HKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
           L   ASG+ ++YKIQPKV    S++F+CYKDVM MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLP+  E+LSQLVVYF++EDYTNYW+HRF H KWGY+KIHRVHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKNSEE 289
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE +K S +
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNK 290

BLAST of ClCG10G003210 vs. TAIR 10
Match: AT4G22753.2 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 428.3 bits (1100), Expect = 5.0e-120
Identity = 197/295 (66.78%), Postives = 249/295 (84.41%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
                   ++KIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EI++QLVVYF++EDYTNYW+HR++H KWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-KKLKEEAKNSEESYYSTA 295
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEEA   EE  + TA
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEA---EEKRFCTA 290

BLAST of ClCG10G003210 vs. TAIR 10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 427.2 bits (1097), Expect = 1.1e-119
Identity = 193/285 (67.72%), Postives = 245/285 (85.96%), Query Frame = 0

Query: 1   MLPFHSISAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A  ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
                   ++KIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEILSQLVVYFMVEDYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EI++QLVVYF++EDYTNYW+HR++H KWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-KKLKEEAK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEEA+
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of ClCG10G003210 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 223.4 bits (568), Expect = 2.4e-58
Identity = 117/264 (44.32%), Postives = 158/264 (59.85%), Query Frame = 0

Query: 19  TFFETLWFNYSHDKSDYFLFCHNILFLF-LIFSFIPLPLIFLELLHASGIHKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPLPSGWEILSQLVVYFMVE 138
            P ++  +C   ++   F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++E
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWMHRFLHGKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFT 258
           +IT WLW+ LR +E ++ H GY FPWSL+ F+P YGGAD HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKILKKLKE 282
           Y D+I+GTDKGYR     LK LKE
Sbjct: 243 YMDWIFGTDKGYRR----LKTLKE 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876881.12.1e-16896.33methylsterol monooxygenase 1-2-like [Benincasa hispida][more]
XP_023552163.14.6e-16895.67methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo][more]
XP_022965134.17.9e-16895.67methylsterol monooxygenase 1-2 [Cucurbita maxima][more]
XP_022923513.11.0e-16795.33methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol m... [more]
XP_011650426.11.5e-16695.00methylsterol monooxygenase 1-2 [Cucumis sativus] >KGN55922.1 hypothetical protei... [more]
Match NameE-valueIdentityDescription
Q1EC699.5e-13274.83Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV... [more]
Q8L7W52.8e-13174.83Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV... [more]
Q9AV393.2e-11970.36Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX... [more]
F4JLZ61.6e-11867.72Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV... [more]
Q8VWZ83.4e-5744.32Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1HQ573.8e-16895.67methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 ... [more]
A0A6J1E9U95.0e-16895.33methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=... [more]
A0A0A0L5P87.2e-16795.00Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BKU61.0e-16595.02methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3... [more]
A0A6J1FUF21.3e-16393.33methylsterol monooxygenase 1-1-like OS=Cucurbita moschata OX=3662 GN=LOC11144822... [more]
Match NameE-valueIdentityDescription
AT4G22756.16.7e-13374.83sterol C4-methyl oxidase 1-2 [more]
AT4G12110.12.0e-13274.83sterol-4alpha-methyl oxidase 1-1 [more]
AT4G22753.25.0e-12066.78sterol 4-alpha methyl oxidase 1-3 [more]
AT4G22753.11.1e-11967.72sterol 4-alpha methyl oxidase 1-3 [more]
AT1G07420.12.4e-5844.32sterol 4-alpha-methyl-oxidase 2-1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 130..265
e-value: 1.0E-29
score: 103.6
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 1..287
NoneNo IPR availablePANTHERPTHR11863:SF197METHYLSTEROL MONOOXYGENASE 1-2coord: 1..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG10G003210.1ClCG10G003210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000254 C-4 methylsterol oxidase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity