ClCG09G000970 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG09G000970
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCG_Chr09: 866901 .. 870432 (-)
RNA-Seq ExpressionClCG09G000970
SyntenyClCG09G000970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCACATCGGAGCAAGCCCTCGTCACTTTACTCTCCCGCTTTGCTTTATCGTTTGATGGAGCCGTTCTGGGTGCTGCCCTAGCTTATGCTGCCGTTCGTACCGTCCTCAAACTCACTGCCTCCTGCTCCTCTCTCAACGAGCTCCGTAAGGCTCCTTATGTTAGCGTTTCTGATCTTCGCCATATTATTCCCGACGGAGATTCGGGACAATCCGAGTCCGATCGAAAGCTTGTTGTTGTTCGTGGCACCGTGGAGGCCAGGTCATCTGTGGAAGGTAATTGGAAGAGCTTACTGCCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGGTAACTCCTTTGGTATTTCTGTTAAGATTCTGCGCGACTGTGTTGAGATTTTGTTTTTTTTTTTGTTTTTTTTTCTTTTGCGTTAGCTGAGTTATGCAGTTTAGAAGATTGCACGATTAAAAATTATTATTCCTCCGTTTGTTAGTGCGTTATGCTTGCAGAGTGTTGTGGCAATATTCGAGTAATTTGATTGCTCCACTAAAATTGCCAACAAAGTCTAAACAATTAATCAACTAACTTGTGCATCTTTTAGATTTTTCACATTACAAGGTCTCTAGTTTAGATACTCGATTCAAGAGGTAGTTATTATGATACGATTCTATCCTGGATGAACCACTATAGAGAAGGATCACTGGGTGAAATAGCTGTTGATTTATCTGTATAGTTCTTCTGGCTTGCCTGAAGTTGATCTGTTGTTGTGTGTGCCTGTGTGTATAGAACTACGATGTGGGGTCTTAAGTATTTAGGAAATCTTGAACTACTTAATTAGCATAACTTCTTTATGAATTTAATCATGGGGGAAATGAGTTTCTCTTTTTTCAGGATTAACTATCGAAATCTCACTGGTTCTGCAAGTGTTTGTATTTGTTCCCCCTCGCTATTCAGTGTGATGGACATGTGTATATGATAATCCAATTCTATAAACTAAAGCTTTTTTGGTTGTATTGTGGCATTGTTCAGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGACTTGCGTGCCATCTTTGGAAGAACTTTTAGAAAGCAAGAGTCGACATCATTTAGAACGGTAAAGTCAAGGCTTATATCTGTTATTGTTATGATTGATTCACTCTTTTCATTACCCTTACCAAAAAAAGATCTTTGTATCAAAAACCTTTTTGTTTAGAATGATATGGGACTTTTTTTTATTCTTTCTGTGAATAAAGAACTAACTTATTGAAGTTGGGGCCATGAGTAGGTTTGTAAGTGTACCTCTTAAGAAATGGGTCCAACAGAGTATGTCTGAAGACACACCCCTTAGTGTCAAGAAATAAGGATTTTATGTTAGCTTACTTATTATATATAAAAGCTAAATCTTCATAACATGAGATTGATGTGTGGACGTTGTGATCTGTGAAGGTGAAATATATATTTTAGATTTCTGGTTGAGCAGCAGGAAACTATATTCCAGTGGTCATTGTACCAGAATTCAGTGCAGTTTGCATATGAAGTCTTATGCGATGCTGATATTTGAGAAAATTTTAACAGGTTCCTTTTGTTCTTGTTGAGGGTCATTCGCCATACTCTGATTTTCTTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTACCCTGTGAGTTAGGCAATCTCTATTGAATTCGAGCACCACCTTTATATATGTATACATATAGATTTTAGCTAAAGATTCTTTAATGTGTTCTACCTTTGAAGGTTGGGGTGCTTGATGAGGAGAAAATTCTTCCATTAGGAAAAGCTATCAGTGCTGTTGGCATATGCAGTTTTGAAAATGGGATTCCTGTGATAAAGTCGTGCAATGATTTTCCCCATTTCCTGTAAGTTTATACATTTTGAAGTAGAGATCATAGATGCATATCATATATTATTTCCCTAGACTCAGCCAATGGTGAACCCAGTAGATTTGGGTTTGGGGCTCTGGATAGGATTTTAACTCAGGATCAAGCCTCCGAGTAAAAAGCTTTTGTTGTCTGTTAGAGCTTAATGGATTTGTGTGTATTTGTAATATCAGTGCTCTCTGCAGTTTAAGCATGGATATTGGTTTGTACCTAAAAACACACGTTTTCTCGTCAGTGATACTAGATAATGGCTAGTCTCTCCTCTCCCACACCCGAAGTAAAAAAAGAAAAAGAAAAAAGATTTCGCTTTTATGTAATAAAACTGAAGCCGTTCGTGATATAGTGCAATTTCTTCTTTGTTGAATATGGGGAATAGTGTAATTGGACAAAAAGTATTTCTGTTTGCGCTTCGTGATAATTGTCATTGGAATAATGGATATTATGATGATATACTCTTTGCAGATGTGAAATGACCAAGGACCAGATGATTTTAGATCTAGTCTTCAAAACAAAATTTCTATTCTGGAGTAGTATTATCCTTGGTTCATTGTCAGTTGTCATTCTTGGTTATTCTGTTGTGAGGTATGTTTTCTTTCTTAATTTCTTCTTTTTCATTTTTCAAGACATCTTTGTGTTGATATTTTTCTTTAGGCAAAGCAACGACTTCTTAATATACCTTCCATGAATTGTTTATTTCTGAATACCTTCCAACTTCCAATTTCCACCAGTCCTTTCATTAGAGACTGTACCCAAGTATACTCCACTTGTCTTGCTTCCTTTCCTTCTTCCTATAATCGTATTTTAAAAGTTAACGAGTATCAATATCCATTCTCTTCAGAAATTGGAATAGATGGAAACAATGGAGGAGGCAACATAGACAGTTACAGAATTCAGGGAATGATTCCACCACGTCACAACTGGATTCAAGCAATGACATTTCCATTTATGGTGATTTTGGAACTGCATCAGACGACGAGGTAGCAGCCATTCCAGACGACGAGTTATCATCAAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTTGTTTGCTGCGAACGTTGTGCCGTCTCAGTTGAACGCGATTCAGCTCCCAAATGTCCCGTCTGCCGCCAGCAAATCCGTAGTTCTTTGCGGATTTACGATTCATGAGGAGAATATAATCATCTTTTCAGATTCTAACCAAGACACATCTACATGGACACATCTCCTTCATCTGGGCTACCCTTTATGCTGATACTGTTTGGGATTTCATATCAATGCCAATCCTTCCACAGGGCAGCAGATCTGAAGTTTGTTTTTTCCACATCTATACTAACTATTTATCCCTATAGTCTTTACTTTGATGACAGAGCTCGTTGTTAAGAGTTTCTAAATCCAAATTTGAAGTGATGGCTCTTGTAAATATCATATATACTCTTTTACTTTATAACATGCTGATATGTGATATGAAAAAGAAACTTCAATATTGGCTCCTG

mRNA sequence

ATGTCCACATCGGAGCAAGCCCTCGTCACTTTACTCTCCCGCTTTGCTTTATCGTTTGATGGAGCCGTTCTGGGTGCTGCCCTAGCTTATGCTGCCGTTCGTACCGTCCTCAAACTCACTGCCTCCTGCTCCTCTCTCAACGAGCTCCGTAAGGCTCCTTATGTTAGCGTTTCTGATCTTCGCCATATTATTCCCGACGGAGATTCGGGACAATCCGAGTCCGATCGAAAGCTTGTTGTTGTTCGTGGCACCGTGGAGGCCAGGTCATCTGTGGAAGGTAATTGGAAGAGCTTACTGCCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGGTAACTCCTTTGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGACTTGCGTGCCATCTTTGGAAGAACTTTTAGAAAGCAAGAGTCGACATCATTTAGAACGGTTCCTTTTGTTCTTGTTGAGGGTCATTCGCCATACTCTGATTTTCTTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATTCTTCCATTAGGAAAAGCTATCAGTGCTGTTGGCATATGCAGTTTTGAAAATGGGATTCCTGTGATAAAGTCGTGCAATGATTTTCCCCATTTCCTATGTGAAATGACCAAGGACCAGATGATTTTAGATCTAGTCTTCAAAACAAAATTTCTATTCTGGAGTAGTATTATCCTTGGTTCATTGTCAGTTGTCATTCTTGGTTATTCTGTTGTGAGAAATTGGAATAGATGGAAACAATGGAGGAGGCAACATAGACAGTTACAGAATTCAGGGAATGATTCCACCACGTCACAACTGGATTCAAGCAATGACATTTCCATTTATGGTGATTTTGGAACTGCATCAGACGACGAGGTAGCAGCCATTCCAGACGACGAGTTATCATCAAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTTGTTTGCTGCGAACGTTGTGCCGTCTCAGTTGAACGCGATTCAGCTCCCAAATGTCCCGTCTGCCGCCAGCAAATCCGTAGTTCTTTGCGGATTTACGATTCATGAGGAGAATATAATCATCTTTTCAGATTCTAACCAAGACACATCTACATGGACACATCTCCTTCATCTGGGCTACCCTTTATGCTGATACTGTTTGGGATTTCATATCAATGCCAATCCTTCCACAGGGCAGCAGATCTGAAGTTTGTTTTTTCCACATCTATACTAACTATTTATCCCTATAGTCTTTACTTTGATGACAGAGCTCGTTGTTAAGAGTTTCTAAATCCAAATTTGAAGTGATGGCTCTTGTAAATATCATATATACTCTTTTACTTTATAACATGCTGATATGTGATATGAAAAAGAAACTTCAATATTGGCTCCTG

Coding sequence (CDS)

ATGTCCACATCGGAGCAAGCCCTCGTCACTTTACTCTCCCGCTTTGCTTTATCGTTTGATGGAGCCGTTCTGGGTGCTGCCCTAGCTTATGCTGCCGTTCGTACCGTCCTCAAACTCACTGCCTCCTGCTCCTCTCTCAACGAGCTCCGTAAGGCTCCTTATGTTAGCGTTTCTGATCTTCGCCATATTATTCCCGACGGAGATTCGGGACAATCCGAGTCCGATCGAAAGCTTGTTGTTGTTCGTGGCACCGTGGAGGCCAGGTCATCTGTGGAAGGTAATTGGAAGAGCTTACTGCCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGGTAACTCCTTTGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGACTTGCGTGCCATCTTTGGAAGAACTTTTAGAAAGCAAGAGTCGACATCATTTAGAACGGTTCCTTTTGTTCTTGTTGAGGGTCATTCGCCATACTCTGATTTTCTTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATTCTTCCATTAGGAAAAGCTATCAGTGCTGTTGGCATATGCAGTTTTGAAAATGGGATTCCTGTGATAAAGTCGTGCAATGATTTTCCCCATTTCCTATGTGAAATGACCAAGGACCAGATGATTTTAGATCTAGTCTTCAAAACAAAATTTCTATTCTGGAGTAGTATTATCCTTGGTTCATTGTCAGTTGTCATTCTTGGTTATTCTGTTGTGAGAAATTGGAATAGATGGAAACAATGGAGGAGGCAACATAGACAGTTACAGAATTCAGGGAATGATTCCACCACGTCACAACTGGATTCAAGCAATGACATTTCCATTTATGGTGATTTTGGAACTGCATCAGACGACGAGGTAGCAGCCATTCCAGACGACGAGTTATCATCAAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTTGTTTGCTGCGAACGTTGTGCCGTCTCAGTTGAACGCGATTCAGCTCCCAAATGTCCCGTCTGCCGCCAGCAAATCCGTAGTTCTTTGCGGATTTACGATTCATGA

Protein sequence

MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDLRHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQVVTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFLVVNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFENGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNRWKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS
Homology
BLAST of ClCG09G000970 vs. NCBI nr
Match: XP_038897215.1 (E3 ubiquitin-protein ligase SPL2 [Benincasa hispida])

HSP 1 Score: 757.7 bits (1955), Expect = 5.3e-215
Identity = 381/412 (92.48%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCS+LNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVGVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSL PN+LISQESGDRAVIIQRTQV
Sbjct: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNILISQESGDRAVIIQRTQV 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFLVVNMDG 180
               CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDF+VVNMDG
Sbjct: 121 ----CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDG 180

Query: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFEN 240
           SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGK ISAVGICSFEN
Sbjct: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFEN 240

Query: 241 GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNRW 300
           G+PVIKSC+DFPHFLCEMTKDQMILDLVFKTKFLFWSSI+LGSLSV ILGYSVVRNWNRW
Sbjct: 241 GVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLSVGILGYSVVRNWNRW 300

Query: 301 KQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLC 360
           KQW RQHR+LQNSGNDSTTSQLDSSNDI IYGDFGTASDDEVA          +PDGQLC
Sbjct: 301 KQW-RQHRRLQNSGNDSTTSQLDSSNDIPIYGDFGTASDDEVA---------TVPDGQLC 360

Query: 361 VICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           VICL RRKRSAFIPCGHFVCCERCAVS+ERDSAPKCPVCRQQIRSS+RIYDS
Sbjct: 361 VICLTRRKRSAFIPCGHFVCCERCAVSIERDSAPKCPVCRQQIRSSVRIYDS 398

BLAST of ClCG09G000970 vs. NCBI nr
Match: XP_008462309.1 (PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KAA0059400.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa] >TYK03927.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa])

HSP 1 Score: 748.4 bits (1931), Expect = 3.2e-212
Identity = 374/412 (90.78%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCS+LNELRKAPYV+VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG+S QSESDRKLVVVRGTVE RSSVEGNWKSL PNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFLVVNMDG 180
               CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV+GHS YSDF+VVNM+G
Sbjct: 121 ----CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEG 180

Query: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFEN 240
           SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGK ISAVGICSFEN
Sbjct: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFEN 240

Query: 241 GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNRW 300
           G+PVIKSC+DFPHFLCEMTKDQMILDLVFKTKFLFWSSI+LGSL+V ILGYS VRNWNRW
Sbjct: 241 GVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRW 300

Query: 301 KQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLC 360
           KQW RQHR+LQNS NDSTTSQLDSSNDI IYGDF  ASDDE A +PDDELSSN+PDGQLC
Sbjct: 301 KQW-RQHRRLQNSRNDSTTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSNVPDGQLC 360

Query: 361 VICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           VICLMRRKRSAFIPCGH VCCERCAVSVERDS+PKCP+CRQQIRSS+RIYDS
Sbjct: 361 VICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

BLAST of ClCG09G000970 vs. NCBI nr
Match: XP_023549225.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 738.0 bits (1904), Expect = 4.3e-209
Identity = 371/413 (89.83%), Postives = 394/413 (95.40%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+SEQALV+LLS+FAL FDGAVLGAALAYAAVRTVLKLTASCS+L+ELRKAPYVSVSDL
Sbjct: 1   MSSSEQALVSLLSQFALXFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+ PNVLISQESGDRAVIIQRTQ 
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQ- 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFLVVNMD 180
              +CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV EGHSPYSDF+VVNMD
Sbjct: 121 ---MCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMD 180

Query: 181 GSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFE 240
           GSRHPLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+
Sbjct: 181 GSRHPLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFK 240

Query: 241 NGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNR 300
           NG+PVIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS++LGSLSV +LGYSVVRNWNR
Sbjct: 241 NGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNR 300

Query: 301 WKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQL 360
           WKQWR+Q R+LQ+SGNDST SQLD SNDI IYGDFGTASD+E AA P+DE+SSN+PDGQL
Sbjct: 301 WKQWRQQ-RRLQDSGNDSTMSQLD-SNDIPIYGDFGTASDNEEAADPEDEVSSNVPDGQL 360

Query: 361 CVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           CVICLMRRKRSAFIPCGHFVCCERCA+SVERDSAPKCPVCRQQIRSS+RIYDS
Sbjct: 361 CVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of ClCG09G000970 vs. NCBI nr
Match: XP_022954158.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita moschata] >XP_022954159.1 E3 ubiquitin-protein ligase SPL2 [Cucurbita moschata])

HSP 1 Score: 736.1 bits (1899), Expect = 1.7e-208
Identity = 370/413 (89.59%), Postives = 394/413 (95.40%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYAAVRTVLKLTASCS+L+ELRKAPYVSVSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+ PNVLISQESGDRAVIIQRTQ 
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQ- 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFLVVNMD 180
              +CIFNEWKGFFGWTSDLRAIFGRTFRKQESTS RTVPFVLV EGHSPYSDF+VVNMD
Sbjct: 121 ---MCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMD 180

Query: 181 GSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFE 240
           GSRHPLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+
Sbjct: 181 GSRHPLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKKISAVGICSFK 240

Query: 241 NGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNR 300
           NG+PVIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS++LGSLSV +LGYSVVRNWNR
Sbjct: 241 NGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNR 300

Query: 301 WKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQL 360
           WKQWR+Q R+LQ+SGNDST SQLD SN+I IYGDFGTASD+E AA P+DE+SSN+PDGQL
Sbjct: 301 WKQWRQQ-RRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQL 360

Query: 361 CVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           CVICLMRRKRSAFIPCGHFVCCERCA+SVERDSAPKCPVCRQQIRSS+RIYDS
Sbjct: 361 CVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of ClCG09G000970 vs. NCBI nr
Match: KAG7013989.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 735.7 bits (1898), Expect = 2.2e-208
Identity = 370/413 (89.59%), Postives = 394/413 (95.40%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYAAVRTVLKLTASCS+L+ELRKAPYVSVSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+ PNVLISQESGDRAVIIQRTQ 
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQ- 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFLVVNMD 180
              +CIFNEWKGFFGWTSDLRAIFGRTFRKQESTS RTVPFVLV EGHSPYSDF+VVNMD
Sbjct: 121 ---MCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMD 180

Query: 181 GSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFE 240
           GSRHPLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+
Sbjct: 181 GSRHPLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFK 240

Query: 241 NGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNR 300
           NG+PVIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS++LGSLSV +LGYSVVRNWNR
Sbjct: 241 NGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNR 300

Query: 301 WKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQL 360
           WKQWR+Q R+LQ+SGNDST SQLD SN+I IYGDFGTASD+E AA P+DE+SSN+PDGQL
Sbjct: 301 WKQWRQQ-RRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQL 360

Query: 361 CVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           CVICLMRRKRSAFIPCGHFVCCERCA+SVERDSAPKCPVCRQQIRSS+RIYDS
Sbjct: 361 CVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of ClCG09G000970 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.2e-98
Identity = 206/419 (49.16%), Postives = 275/419 (65.63%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+ E+AL+ LL+  ALSFDGA+LG  LA +AV + LK  ++ ++L +++ AP VS+SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIIP------DGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVI 120
           R ++P      + +  +  +D+++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQVVTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDF 180
           IQRTQ      +++ WK  F  T   R +  R+ RK  +   RTVPFV+V +     S F
Sbjct: 121 IQRTQTY----VYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSF 180

Query: 181 LVVNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAV 240
           + VNMDGSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK I+AV
Sbjct: 181 VAVNMDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAV 240

Query: 241 GICSFENGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSV 300
           GI SF NG+P IKSC D P+FL EMTKD+MI DL+ +T F+F  S+ILG +SV IL Y+ 
Sbjct: 241 GIYSFNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAA 300

Query: 301 VRNWNRWKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSN 360
           VR WN+WKQW  Q R+L    NDS                     DDE    P+D  +  
Sbjct: 301 VRTWNKWKQWNHQ-RELPQRPNDSVV-------------------DDE----PED--ADE 360

Query: 361 IPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           IPDG+LCVIC+ RR+  AFIPCGH VCC RCA +VER+  PKCPVC Q IR S+R+Y S
Sbjct: 361 IPDGELCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

BLAST of ClCG09G000970 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.8e-08
Identity = 34/105 (32.38%), Postives = 57/105 (54.29%), Query Frame = 0

Query: 314 GNDSTTS------QLDSS--NDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLCVICLM 373
           GN + TS      ++D +   DI +  D  +   D++AA+P +E    + + ++C +C+ 
Sbjct: 499 GNTAATSFRNSLREIDPALYRDIFVQQDIRSLPTDDIAALPMEEQLRKLQEERMCKVCMD 558

Query: 374 RRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIY 411
           R     FIPCGH V C+ CA S+      KCP+CR  I+ ++R +
Sbjct: 559 REVSIVFIPCGHLVVCKDCAPSLR-----KCPICRGTIKGTVRTF 598

BLAST of ClCG09G000970 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-07
Identity = 27/62 (43.55%), Postives = 38/62 (61.29%), Query Frame = 0

Query: 351 SSNIPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIY 410
           S++IP+ +LC IC      +AF+PCGH V C +CA SV      KCP+CR+     +R+Y
Sbjct: 382 STSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVY 438

Query: 411 DS 413
            S
Sbjct: 442 FS 438

BLAST of ClCG09G000970 vs. ExPASy Swiss-Prot
Match: P41436 (Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=IAP PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.5e-07
Identity = 26/64 (40.62%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 349 ELSSNIPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLR 408
           E    + D +LC IC +      F+PCGH V C +CA+SV+     KCP+CR+ + S L+
Sbjct: 217 EKEPQVEDSKLCKICYVEECIVCFVPCGHVVACAKCALSVD-----KCPMCRKIVTSVLK 275

Query: 409 IYDS 413
           +Y S
Sbjct: 277 VYFS 275

BLAST of ClCG09G000970 vs. ExPASy Swiss-Prot
Match: Q94HV7 (E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.4e-07
Identity = 57/239 (23.85%), Postives = 108/239 (45.19%), Query Frame = 0

Query: 176 VNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYP---VGVLDEEKILPLGKAISA 235
           VN+D S+  L L           A P + +Q   G+      +GV   E+++P+G  ++ 
Sbjct: 120 VNVDVSQGELGLALTVGSDVFEKAEPVSLVQGALGYLKGFKILGVRHVERVVPIGTPLTV 179

Query: 236 VGICSFEN-GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGY 295
           VG    +  G   I+     P ++  +  DQ+I  L   ++   ++S+ L  L V+++  
Sbjct: 180 VGEAVRDGMGNVRIQKPEQGPFYVTYIPLDQLISKLGDLSRRFKYASMGLTVLGVILISK 239

Query: 296 SVVRNWNRWKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELS 355
            V+    +  +   + R+ Q +      +    +  ++     GT+ D +          
Sbjct: 240 PVIEYILKRIEDTLERRRRQFALKRVVDAAARRAKPVT---GGGTSRDGDT--------- 299

Query: 356 SNIPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIY 411
              PD  LCV+CL ++  +AF+ CGH  CC  C++ +       CP+CR++I+  L+IY
Sbjct: 300 ---PD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-----TCPLCRERIQQVLKIY 336

BLAST of ClCG09G000970 vs. ExPASy TrEMBL
Match: A0A5D3BWQ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001240 PE=4 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.6e-212
Identity = 374/412 (90.78%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCS+LNELRKAPYV+VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG+S QSESDRKLVVVRGTVE RSSVEGNWKSL PNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFLVVNMDG 180
               CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV+GHS YSDF+VVNM+G
Sbjct: 121 ----CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEG 180

Query: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFEN 240
           SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGK ISAVGICSFEN
Sbjct: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFEN 240

Query: 241 GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNRW 300
           G+PVIKSC+DFPHFLCEMTKDQMILDLVFKTKFLFWSSI+LGSL+V ILGYS VRNWNRW
Sbjct: 241 GVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRW 300

Query: 301 KQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLC 360
           KQW RQHR+LQNS NDSTTSQLDSSNDI IYGDF  ASDDE A +PDDELSSN+PDGQLC
Sbjct: 301 KQW-RQHRRLQNSRNDSTTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSNVPDGQLC 360

Query: 361 VICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           VICLMRRKRSAFIPCGH VCCERCAVSVERDS+PKCP+CRQQIRSS+RIYDS
Sbjct: 361 VICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

BLAST of ClCG09G000970 vs. ExPASy TrEMBL
Match: A0A1S3CGP0 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.6e-212
Identity = 374/412 (90.78%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCS+LNELRKAPYV+VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG+S QSESDRKLVVVRGTVE RSSVEGNWKSL PNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFLVVNMDG 180
               CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV+GHS YSDF+VVNM+G
Sbjct: 121 ----CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEG 180

Query: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFEN 240
           SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGK ISAVGICSFEN
Sbjct: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFEN 240

Query: 241 GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNRW 300
           G+PVIKSC+DFPHFLCEMTKDQMILDLVFKTKFLFWSSI+LGSL+V ILGYS VRNWNRW
Sbjct: 241 GVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRW 300

Query: 301 KQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLC 360
           KQW RQHR+LQNS NDSTTSQLDSSNDI IYGDF  ASDDE A +PDDELSSN+PDGQLC
Sbjct: 301 KQW-RQHRRLQNSRNDSTTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSNVPDGQLC 360

Query: 361 VICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           VICLMRRKRSAFIPCGH VCCERCAVSVERDS+PKCP+CRQQIRSS+RIYDS
Sbjct: 361 VICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

BLAST of ClCG09G000970 vs. ExPASy TrEMBL
Match: A0A6J1GS48 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504 PE=4 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 8.0e-209
Identity = 370/413 (89.59%), Postives = 394/413 (95.40%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYAAVRTVLKLTASCS+L+ELRKAPYVSVSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+ PNVLISQESGDRAVIIQRTQ 
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQ- 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFLVVNMD 180
              +CIFNEWKGFFGWTSDLRAIFGRTFRKQESTS RTVPFVLV EGHSPYSDF+VVNMD
Sbjct: 121 ---MCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMD 180

Query: 181 GSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFE 240
           GSRHPLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+
Sbjct: 181 GSRHPLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKKISAVGICSFK 240

Query: 241 NGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNR 300
           NG+PVIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS++LGSLSV +LGYSVVRNWNR
Sbjct: 241 NGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNR 300

Query: 301 WKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQL 360
           WKQWR+Q R+LQ+SGNDST SQLD SN+I IYGDFGTASD+E AA P+DE+SSN+PDGQL
Sbjct: 301 WKQWRQQ-RRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQL 360

Query: 361 CVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           CVICLMRRKRSAFIPCGHFVCCERCA+SVERDSAPKCPVCRQQIRSS+RIYDS
Sbjct: 361 CVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of ClCG09G000970 vs. ExPASy TrEMBL
Match: A0A0A0K708 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE=4 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.0e-208
Identity = 370/412 (89.81%), Postives = 387/412 (93.93%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCS+LNELRKAPYV+VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG S QSESDRKLVVVRGTVE RSSVEGN KSL PNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFLVVNMDG 180
               CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+EGHS YSDF+VVNM+G
Sbjct: 121 ----CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEG 180

Query: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFEN 240
           SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGK ISAVGICSFEN
Sbjct: 181 SRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFEN 240

Query: 241 GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNRW 300
           G+PVIKSC+DFPHFLCEMTKDQMILDLVFKTKFLFWSSI+LGSL+V ILGYS  RNWNRW
Sbjct: 241 GVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRW 300

Query: 301 KQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLC 360
           KQW RQHRQLQNS NDS TSQLDSSNDI IYGDF  ASDDE A +PDDELSS++PDGQLC
Sbjct: 301 KQW-RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDGQLC 360

Query: 361 VICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           VICLMRRKRSAFIPCGH VCCERCAVSVER+S+PKCP+CRQQIRSS+RIYDS
Sbjct: 361 VICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 405

BLAST of ClCG09G000970 vs. ExPASy TrEMBL
Match: A0A6J1JT39 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.8e-208
Identity = 369/413 (89.35%), Postives = 394/413 (95.40%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYAAVRTVLKLTASCS+L+ELRKAPYVSVSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSG+SESD KLVVVRGTVEARSSVEGNWKS+ PNVLISQESGDRAVIIQRTQ 
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQ- 120

Query: 121 VTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFLVVNMD 180
              +CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV EGHSPYSDF+VVNMD
Sbjct: 121 ---MCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMD 180

Query: 181 GSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAVGICSFE 240
           GSRHPLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+
Sbjct: 181 GSRHPLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFK 240

Query: 241 NGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSVVRNWNR 300
           NG+PVIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS++LGSLSV +LGYSVVRNWNR
Sbjct: 241 NGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNR 300

Query: 301 WKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQL 360
           WKQWR+Q R+LQ+SGNDST SQLD SN+I IYGDFGTASD+E A  P+DE+SSN+PDGQL
Sbjct: 301 WKQWRQQ-RRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAVDPEDEVSSNVPDGQL 360

Query: 361 CVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           CVICLMRRKRSAFIPCGHFVCCERCA+SVERDSAPKCPVCRQQIRSS+RIYDS
Sbjct: 361 CVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of ClCG09G000970 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 360.1 bits (923), Expect = 2.3e-99
Identity = 206/419 (49.16%), Postives = 275/419 (65.63%), Query Frame = 0

Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSSLNELRKAPYVSVSDL 60
           MS+ E+AL+ LL+  ALSFDGA+LG  LA +AV + LK  ++ ++L +++ AP VS+SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIIP------DGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLLPNVLISQESGDRAVI 120
           R ++P      + +  +  +D+++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQVVTPLCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDF 180
           IQRTQ      +++ WK  F  T   R +  R+ RK  +   RTVPFV+V +     S F
Sbjct: 121 IQRTQTY----VYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSF 180

Query: 181 LVVNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKAISAV 240
           + VNMDGSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK I+AV
Sbjct: 181 VAVNMDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAV 240

Query: 241 GICSFENGIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGYSV 300
           GI SF NG+P IKSC D P+FL EMTKD+MI DL+ +T F+F  S+ILG +SV IL Y+ 
Sbjct: 241 GIYSFNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAA 300

Query: 301 VRNWNRWKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSN 360
           VR WN+WKQW  Q R+L    NDS                     DDE    P+D  +  
Sbjct: 301 VRTWNKWKQWNHQ-RELPQRPNDSVV-------------------DDE----PED--ADE 360

Query: 361 IPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIYDS 413
           IPDG+LCVIC+ RR+  AFIPCGH VCC RCA +VER+  PKCPVC Q IR S+R+Y S
Sbjct: 361 IPDGELCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

BLAST of ClCG09G000970 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.4e-08
Identity = 57/239 (23.85%), Postives = 108/239 (45.19%), Query Frame = 0

Query: 176 VNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYP---VGVLDEEKILPLGKAISA 235
           VN+D S+  L L           A P + +Q   G+      +GV   E+++P+G  ++ 
Sbjct: 120 VNVDVSQGELGLALTVGSDVFEKAEPVSLVQGALGYLKGFKILGVRHVERVVPIGTPLTV 179

Query: 236 VGICSFEN-GIPVIKSCNDFPHFLCEMTKDQMILDLVFKTKFLFWSSIILGSLSVVILGY 295
           VG    +  G   I+     P ++  +  DQ+I  L   ++   ++S+ L  L V+++  
Sbjct: 180 VGEAVRDGMGNVRIQKPEQGPFYVTYIPLDQLISKLGDLSRRFKYASMGLTVLGVILISK 239

Query: 296 SVVRNWNRWKQWRRQHRQLQNSGNDSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELS 355
            V+    +  +   + R+ Q +      +    +  ++     GT+ D +          
Sbjct: 240 PVIEYILKRIEDTLERRRRQFALKRVVDAAARRAKPVT---GGGTSRDGDT--------- 299

Query: 356 SNIPDGQLCVICLMRRKRSAFIPCGHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRIY 411
              PD  LCV+CL ++  +AF+ CGH  CC  C++ +       CP+CR++I+  L+IY
Sbjct: 300 ---PD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-----TCPLCRERIQQVLKIY 336

BLAST of ClCG09G000970 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 7.1e-08
Identity = 31/94 (32.98%), Postives = 46/94 (48.94%), Query Frame = 0

Query: 316 DSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLCVICLMRRKRSAFIPC 375
           D T   ++ S +++  GD  TAS            S ++    LCVIC   R+   F+PC
Sbjct: 34  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 93

Query: 376 GHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRI 410
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 94  GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 118

BLAST of ClCG09G000970 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 7.1e-08
Identity = 31/94 (32.98%), Postives = 46/94 (48.94%), Query Frame = 0

Query: 316 DSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLCVICLMRRKRSAFIPC 375
           D T   ++ S +++  GD  TAS            S ++    LCVIC   R+   F+PC
Sbjct: 68  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 127

Query: 376 GHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRI 410
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 128 GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 152

BLAST of ClCG09G000970 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 7.1e-08
Identity = 31/94 (32.98%), Postives = 46/94 (48.94%), Query Frame = 0

Query: 316 DSTTSQLDSSNDISIYGDFGTASDDEVAAIPDDELSSNIPDGQLCVICLMRRKRSAFIPC 375
           D T   ++ S +++  GD  TAS            S ++    LCVIC   R+   F+PC
Sbjct: 89  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 148

Query: 376 GHFVCCERCAVSVERDSAPKCPVCRQQIRSSLRI 410
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 149 GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897215.15.3e-21592.48E3 ubiquitin-protein ligase SPL2 [Benincasa hispida][more]
XP_008462309.13.2e-21290.78PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KA... [more]
XP_023549225.14.3e-20989.83LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pep... [more]
XP_022954158.11.7e-20889.59E3 ubiquitin-protein ligase SPL2 [Cucurbita moschata] >XP_022954159.1 E3 ubiquit... [more]
KAG7013989.12.2e-20889.59E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9SYH33.2e-9849.16E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV... [more]
O088631.8e-0832.38Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE... [more]
Q243061.2e-0743.55Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=... [more]
P414361.5e-0740.63Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963... [more]
Q94HV73.4e-0723.85E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A5D3BWQ01.6e-21290.78RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CGP01.6e-21290.78RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 ... [more]
A0A6J1GS488.0e-20989.59RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504... [more]
A0A0A0K7081.0e-20889.81RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE... [more]
A0A6J1JT391.8e-20889.35RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 P... [more]
Match NameE-valueIdentityDescription
AT1G54150.12.3e-9949.16E3 Ubiquitin ligase family protein [more]
AT1G59560.12.4e-0823.85E3 Ubiquitin ligase family protein [more]
AT4G24204.17.1e-0832.98RING/U-box superfamily protein [more]
AT4G24204.27.1e-0832.98RING/U-box superfamily protein [more]
AT4G24204.37.1e-0832.98RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 360..399
e-value: 0.0023
score: 27.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 360..400
score: 10.792683
IPR022170E3 Ubiquitin ligase MUL1-likePFAMPF12483GIDEcoord: 146..288
e-value: 2.4E-33
score: 114.8
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 358..405
e-value: 6.5E-14
score: 51.5
NoneNo IPR availablePANTHERPTHR47355:SF1E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..412
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 354..406
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 335..411
e-value: 8.4E-17
score: 62.4
IPR044247E3 ubiquitin-protein ligase SPL2-likePANTHERPTHR47355E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..412

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG09G000970.2ClCG09G000970.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006996 organelle organization
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016874 ligase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity