ClCG08G017430 (gene) Watermelon (Charleston Gray) v2.5

BLAST of ClCG08G017430 vs. NCBI nr
Match: KAA0061032.1 (vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 799.3 bits (2063), Expect = 2.2e-227
Identity = 387/444 (87.16%), Postives = 413/444 (93.02%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTG-RGEDLDF 184
           ++LEVDIISEELIKPSTPTP HLR Y LSFIDQITVHIYAP LYFYQSTDTG  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYPPLAGRPNYAS+FIDCND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V GVFKPHMEGAKIFPPK MSYDTAMT  RNRV+RRFVFKQSKLEAIRAKY E+Q  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 365 NQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYFN 424
           N+PSR+ESLTAF YSRFLA FKHDS+I NDMS+L+NYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 425 LMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNGE 484
           +MIFPS E LNDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM+TV E+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 485 IISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQHM 544
           IISCAFSS CRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSKD + +DAIVHLE+++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 545 NKLEADELFLKYATPIPTLSSPLA 568
           NKLE DE+F+ YATP+PT SSPLA
Sbjct: 421 NKLEGDEVFMNYATPLPTPSSPLA 444

BLAST of ClCG08G017430 vs. NCBI nr
Match: XP_004143048.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical protein Csa_018752 [Cucumis sativus])

HSP 1 Score: 793.1 bits (2047), Expect = 1.6e-225
Identity = 386/445 (86.74%), Postives = 413/445 (92.81%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTG-RGEDLDF 184
           ++LEVDIISEELIKPSTPTP HLR Y LSFIDQITVHIYAP LYFYQSTDTG  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYPPLAGRPNYAS+FIDCND GVPFREA VNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V GVFKPHMEGAKIFPPK MSYD+AMT  RNRV+RRFVFKQSK+EAIR KY E+Q MINQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 365 N-QPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYF 424
           N QPSR+ESLTAF YSRFLA FKHDS I NDMS+L+NYTVNLRPKMNPPLPHDAFGNYYF
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKHDSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300

Query: 425 NLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNG 484
           N+MIFPS E LNDDENCYGLVKQLREETNKI+GEMAKKFLNED++LMKTV E+ASKVV+G
Sbjct: 301 NVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVVSG 360

Query: 485 EIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQH 544
           EI+SCAFSS CRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSKD + IDAIVHLE+++
Sbjct: 361 EIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEERY 420

Query: 545 MNKLEADELFLKYATPIPTLSSPLA 568
           MNKLE DE+F+KYATPIPT SS +A
Sbjct: 421 MNKLEGDEVFMKYATPIPTPSSRVA 445

BLAST of ClCG08G017430 vs. NCBI nr
Match: XP_038886173.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 786.2 bits (2029), Expect = 1.9e-223
Identity = 385/444 (86.71%), Postives = 412/444 (92.79%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGR-GEDLDF 184
           ++LEVDIISEELIKPSTPTP HLRRYPLS IDQITVHIYAP LYFY+STD+G  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRRYPLSLIDQITVHIYAPTLYFYRSTDSGSGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYP LAGRPNYAS+FI+CNDAGVPFREARVNSQLADVVQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPQLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V  VFKPHMEGAKIFP KTMSYDTAMT  RNRVARRFVF+QSKLEAIRAKYAE+QA INQ
Sbjct: 181 VKDVFKPHMEGAKIFPAKTMSYDTAMTLVRNRVARRFVFEQSKLEAIRAKYAENQATINQ 240

Query: 365 NQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYFN 424
            +PSR+ESL+AF YSRF+A  KH S++ ND+S+LINYTVNLRPKMNPPLPHDAFGNYYFN
Sbjct: 241 IRPSRIESLSAFVYSRFIAALKHGSAMSNDVSFLINYTVNLRPKMNPPLPHDAFGNYYFN 300

Query: 425 LMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNGE 484
           +MIFPS EMLN DENCYGLVKQLREETNKINGEMAKKFLNE++DLM+TV + ASKVVNGE
Sbjct: 301 VMIFPSPEMLNTDENCYGLVKQLREETNKINGEMAKKFLNENKDLMETVKDFASKVVNGE 360

Query: 485 IISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQHM 544
           IISCAFSSTCR PLYDVDFGWGRPVWVTFP LWFKNLV FL+SKD D IDAIVHLE+Q+M
Sbjct: 361 IISCAFSSTCRLPLYDVDFGWGRPVWVTFPGLWFKNLVAFLESKDGDGIDAIVHLEEQYM 420

Query: 545 NKLEADELFLKYATPIPTLSSPLA 568
           NKLEADE+FLKYA  IPT SSPLA
Sbjct: 421 NKLEADEVFLKYAATIPTPSSPLA 444

BLAST of ClCG08G017430 vs. NCBI nr
Match: XP_008445555.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 770.0 bits (1987), Expect = 1.4e-218
Identity = 373/424 (87.97%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTG-RGEDLDF 184
           ++LEVDIISEELIKPSTPTP HLR Y LSFIDQITVHIYAP LYFYQSTDTG  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYPPLAGRPNYAS+FIDCND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V GVFKPHMEGAKIFPPK MSYDTAMT  RNRV+RRFVFKQSKLEAIRAKY E+Q  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 365 NQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYFN 424
           N+PSR+ESLTAF YSRFLA FKHDS+I NDMS+L+NYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 425 LMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNGE 484
           +MIFPS E LNDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM+TV E+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 485 IISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQHM 544
           IISCAFSS CRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSKD + +DAIVHLE+++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 545 NKLE 548
           NKLE
Sbjct: 421 NKLE 424

BLAST of ClCG08G017430 vs. NCBI nr
Match: KAG7020113.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 668.7 bits (1724), Expect = 4.5e-188
Identity = 330/439 (75.17%), Postives = 373/439 (84.97%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFA 184
           ++LE+DIISE+LIKP+TPTP HLRRYPLSFIDQITVHIYAP LYFYQ    G   D DFA
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAG---DPDFA 60

Query: 185 LAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPD 244
           + F+NRLR+TL  VLSHYPPLAGRPNY S F+DCND G  FREARVN++LADVV++A+PD
Sbjct: 61  IDFRNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPD 120

Query: 245 DLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARGV 304
           DLNRLFPVELD+FNEELMAVQFTEFACGGVA+ASCISHKIAD M LFSLNNNWAAMARGV
Sbjct: 121 DLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGV 180

Query: 305 NGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQN 364
            GVFKPHMEGAK+FP KTM+YDTA T  RNRVARRFVF+ SK+EAIR KY E+ A    +
Sbjct: 181 KGVFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AH 240

Query: 365 QPSRVESLTAFFYSRFLATFKHDSS--IPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYF 424
           +PSRVESLTAF Y RFLA  K  SS  +P+D + L+NYTVNLR KM PPLP DAFGNYYF
Sbjct: 241 RPSRVESLTAFMYGRFLAALKDGSSSEVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYF 300

Query: 425 NLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNG 484
           N MIFPS ++LN D  CYGLV +LREETNK+N +  K  LNED+ LM++V EIASK V G
Sbjct: 301 NAMIFPSPDILN-DPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKG 360

Query: 485 EIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQH 544
           E+ISCAF+S CRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSK++  IDAIVHLE+ H
Sbjct: 361 EMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPH 420

Query: 545 MNKLEADELFLKYATPIPT 562
           MNKL+AD+LFL+YATP PT
Sbjct: 421 MNKLQADQLFLQYATPTPT 432

BLAST of ClCG08G017430 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.1e-62
Identity = 153/434 (35.25%), Postives = 239/434 (55.07%), Query Frame = 0

Query: 128 EVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFALAF 187
           ++ I+SEELI+PS+PTP  L+ + LS +DQ+ +  + P++ FY +       +LD A   
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQ---LDSNLDRAQRS 63

Query: 188 KNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPDDLN 247
           +N L+ +L  VL+ + PLAGR N  S+ +DCND+GVPF EARV+SQL++ ++    D+LN
Sbjct: 64  EN-LKRSLSTVLTQFYPLAGRININSS-VDCNDSGVPFLEARVHSQLSEAIKNVAIDELN 123

Query: 248 RLFPVELDRFNEEL-------MAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAM 307
           +  P +     EE        +AV+ + F CGG AI  CISHKIAD ++L +  N+W A 
Sbjct: 124 QYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTAT 183

Query: 308 ARGVNGVFKPHME-GAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQA 367
            +    + +P+ + G+  FPP             N V +RFVF + KLEA++A+ A S  
Sbjct: 184 CQEETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSAT 243

Query: 368 MINQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGN 427
            +  +  SRV+ + A  + +F+   +      N +  +    VNLR +MNPP P  A GN
Sbjct: 244 EVKNS--SRVQIVIAVIWKQFIDVTRAKFDTKNKL--VAAQAVNLRSRMNPPFPQSAMGN 303

Query: 428 YYFNLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKV 487
                +   +  +  +D++   LV  L+    KI+ E  K       +L K VT +  + 
Sbjct: 304 -----IATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVK-------ELQKGVTYLDYEA 363

Query: 488 VNGEIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLE 547
              E+ S  FSS CR   YD+DFGWG+PV V    +  KNLV  +D+++ED ++A + + 
Sbjct: 364 EPQELFS--FSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMA 414

Query: 548 QQHMNKLEADELFL 554
           +  M+ L +D L L
Sbjct: 424 EDEMSMLSSDFLSL 414

BLAST of ClCG08G017430 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 3.4e-50
Identity = 141/437 (32.27%), Postives = 229/437 (52.40%), Query Frame = 0

Query: 128 EVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFALAF 187
           +++ +SEELI PS+PTP  L+ Y +S +DQ+ +  + P + FY +       +LD A   
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNP---LDSNLDPAQT- 63

Query: 188 KNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLA----DVVQFAQP 247
              L+ +L  VL+H+ PLAGR N  S+ +DCND+GVPF EARV +QL+    +VV+  + 
Sbjct: 64  SQHLKQSLSKVLTHFYPLAGRINVNSS-VDCNDSGVPFVEARVQAQLSQAIQNVVELEKL 123

Query: 248 DDL--NRLFPVELDRFNEEL-MAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAM 307
           D    +  +P      NE++ +AV+ + F CGG AI   +SHKIAD ++L +  N W A 
Sbjct: 124 DQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTAT 183

Query: 308 ARGVNGVFKPHME-GAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQA 367
            RG   +  P+ +  A+ FPP   +    +    N V +RFVF + K+ A+R   A++ +
Sbjct: 184 CRGETEIVLPNFDLAARHFPPVDNTPSPELVPDENVVMKRFVFDKEKIGALR---AQASS 243

Query: 368 MINQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGN 427
              +   SRV+ + A+ +   +   +      N   +++   VNLR +MNPPLPH A GN
Sbjct: 244 ASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKN--KFVVVQAVNLRSRMNPPLPHYAMGN 303

Query: 428 YYFNLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKV 487
               L      E   D  +  G ++   E+T            + + +L+K +T    ++
Sbjct: 304 IATLLFAAVDAEWDKDFPDLIGPLRTSLEKTED----------DHNHELLKGMT-CLYEL 363

Query: 488 VNGEIISCAFSSTCRFPLYDVDFGWGRPV---WVTFPALWFKNLVTFLDSKDEDAIDAIV 547
              E++S  F+S CR   YD+DFGWG+P+     TFP    +N    +D++  D ++A +
Sbjct: 364 EPQELLS--FTSWCRLGFYDLDFGWGKPLSACTTTFPK---RNAALLMDTRSGDGVEAWL 414

Query: 548 HLEQQHMNKLEADELFL 554
            + +  M  L  + L L
Sbjct: 424 PMAEDEMAMLPVELLSL 414

BLAST of ClCG08G017430 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 5.0e-49
Identity = 147/454 (32.38%), Postives = 236/454 (51.98%), Query Frame = 0

Query: 124 RLELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDF 183
           ++ +E + +S+ LIKPS+PTP  L RY LS+ DQ        V +FY++ D   G ++  
Sbjct: 5   KISVETETLSKTLIKPSSPTPQSLSRYNLSYNDQNIYQTCVSVGFFYENPD---GIEIS- 64

Query: 184 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQF--- 243
               + +L+ +L   L  Y P AG+    +++I CND G+ F E R+  ++ D++++   
Sbjct: 65  --TIREQLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVEVRIRCRMNDILKYELR 124

Query: 244 AQPDDLNRLFPVELDRFNEELMA-VQFTEFACGGVAIASCISHKIADGMALFSLNNNWAA 303
           +   DL  + P  +   +E+  A VQ + F CGG+A+A  ISHK+ADG  + S   +WAA
Sbjct: 125 SYARDL--VLPKRVTVGSEDTTAIVQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAA 184

Query: 304 MA---RGVNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAE 363
            A      + V  P +    IFP +         T +N V + F+F    +E +++K  E
Sbjct: 185 SACYLSSSHHVPTPLLVSDSIFPRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLKSKAVE 244

Query: 364 SQAMINQNQPSRVESLTAFFYSRFLATFKHDSSIPND-----MSYLINYTVNLRPKMNPP 423
                   +P+RVE LTAF  SR  AT    S+  N+     + + +   +NLRP +   
Sbjct: 245 ----FGIEKPTRVEVLTAFL-SR-CATVAGKSAAKNNNCGQSLPFPVLQAINLRPILE-- 304

Query: 424 LPHDAFGN---YYFNLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKK--FLNEDE 483
           LP ++ GN    YF+  I    + LN+ E    ++ +LR+E  KI     +K  ++ + E
Sbjct: 305 LPQNSVGNLVSIYFSRTI-KENDYLNEKEYTKLVINELRKEKQKIKNLSREKLTYVAQME 364

Query: 484 DLMKTVTEIASKVVNGEIISCAFS-STCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLD 543
           + +K++ E    + N   I    S S CRFP YDVDFGWG+P+WV     + KN V  +D
Sbjct: 365 EFVKSLKEF--DISNFLDIDAYLSDSWCRFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMD 424

Query: 544 SK--DEDAIDAIVHLEQQHMNKLEADELFLKYAT 558
               D+  I+AIV  EQ+ M+  E +E  L++ +
Sbjct: 425 YPFGDDYGIEAIVSFEQEKMSAFEKNEQLLQFVS 438

BLAST of ClCG08G017430 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.4e-46
Identity = 131/436 (30.05%), Postives = 220/436 (50.46%), Query Frame = 0

Query: 129 VDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFALAFK 188
           V+I+S+  IKPS+PTP  LRRY LS IDQ        + +FY    +     +       
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIG------ 65

Query: 189 NRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPDDLNR 248
           + L+ +L   L  Y   AGR    +++IDCND GV F E R++ ++ D+++  +    + 
Sbjct: 66  DLLKNSLSKTLVSYYQFAGR-LIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDL 125

Query: 249 LFPVELDRFNEE-LMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARGVNGV 308
           + P  +   +E+ L+ VQ + F CGG+AI    SHK++DG++      +WA+ +  ++  
Sbjct: 126 VLPTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWAS-STSLSTF 185

Query: 309 FKPH--MEGAKIFPPK--TMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 368
            KP   +    IFPP+   +  + ++ + +  + +RFVF    +E +++K  E       
Sbjct: 186 HKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE----YGI 245

Query: 369 NQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYFN 428
            +PSRVE +TA     FL     +  +P    Y I   VNLRP +   LP ++ GN    
Sbjct: 246 QKPSRVEVVTA-----FLCQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGN---- 305

Query: 429 LMIFPSQEMLND---DENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVV 488
             IF     +ND   D     L+ +LR    K+   +  K     E  M+ +T+   ++ 
Sbjct: 306 --IFSFYFCINDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQLN 365

Query: 489 NGEIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQ 548
              + +   SS CRFP+YD+DFGWG+P+ V+      K+L+  +DS + D I+A++ +E+
Sbjct: 366 ISSLDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALITMEE 416

Query: 549 QHMNKLEADELFLKYA 557
           + M   E +E    +A
Sbjct: 426 KKMAAFEKNEELRSFA 416

BLAST of ClCG08G017430 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.4e-43
Identity = 140/458 (30.57%), Postives = 227/458 (49.56%), Query Frame = 0

Query: 127 LEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFALA 186
           +E + +S+ LIKPS+PTP  L  Y LS+ DQ     Y    +FY + D   G ++     
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFYSNPD---GHEIS---T 66

Query: 187 FKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPDDL 246
            + +L+ +L   L  Y P AG+    +++I CND G+ F + R++ ++ D++   +P+  
Sbjct: 67  IREQLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVDVRIHCRMNDIL---KPE-- 126

Query: 247 NRLFPVELDRFNEELMA-------VQFTEFACGGVAIASCISHKIADGMALFSLNNNWAA 306
            R +  EL R N   +        VQ + F CGGVA+A  ISHK+AD   + S   +WAA
Sbjct: 127 LRSYASELIRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWAA 186

Query: 307 MARGVNG---VFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAE 366
               ++    V  P +    IFP +            N V +RF+F    +E +++K  E
Sbjct: 187 STCDLSSSHDVSTPVLVSDSIFPRQDNIICGQFPASPNCVRKRFLFSPEAIERLKSKAIE 246

Query: 367 SQAMINQNQPSRVESLTAFFYSRFLATFKHDSSIPND-----MSYLINYTVNLRPKMNPP 426
                   +P+RVE LTAF      AT    S+  N+     + + +   VNLRP +   
Sbjct: 247 ----FGIEKPTRVEVLTAFLCR--CATVAGKSAAKNNNCGQSLPFAVIQAVNLRPLLE-- 306

Query: 427 LPHDAFGN---YYFNLMIFPSQEMLNDDENCYGLV-KQLREETNKINGEMAKK--FLNED 486
           LP ++ GN    YF+ +     + +N ++    LV  +LR+  +K+     +K  ++   
Sbjct: 307 LPKNSVGNLISIYFSTI--KENDTVNIEQEFTKLVIGELRKAKDKLKNLSQEKLNYVARM 366

Query: 487 EDLMKTVTEIASK----VVNGEIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLV 546
           +D    + E+       + N +I +  FSS CRFP YD+DFG G+P+WV      FKN +
Sbjct: 367 QDFANCLKELDISSFFDMENVDIDAYLFSSWCRFPFYDIDFGLGKPIWVCMFQPHFKNCI 426

Query: 547 TFLDSK--DEDAIDAIVHLEQQHMNKLEADELFLKYAT 558
             +D    D+  I+A++ LEQ+ M   E +EL L +A+
Sbjct: 427 ILMDYPFGDDYGIEALITLEQEKMPAFENNELLLSFAS 442

BLAST of ClCG08G017430 vs. ExPASy TrEMBL
Match: A0A5A7UYQ7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001680 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 1.1e-227
Identity = 387/444 (87.16%), Postives = 413/444 (93.02%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTG-RGEDLDF 184
           ++LEVDIISEELIKPSTPTP HLR Y LSFIDQITVHIYAP LYFYQSTDTG  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYPPLAGRPNYAS+FIDCND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V GVFKPHMEGAKIFPPK MSYDTAMT  RNRV+RRFVFKQSKLEAIRAKY E+Q  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 365 NQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYFN 424
           N+PSR+ESLTAF YSRFLA FKHDS+I NDMS+L+NYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 425 LMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNGE 484
           +MIFPS E LNDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM+TV E+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 485 IISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQHM 544
           IISCAFSS CRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSKD + +DAIVHLE+++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 545 NKLEADELFLKYATPIPTLSSPLA 568
           NKLE DE+F+ YATP+PT SSPLA
Sbjct: 421 NKLEGDEVFMNYATPLPTPSSPLA 444

BLAST of ClCG08G017430 vs. ExPASy TrEMBL
Match: A0A0A0LQS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 7.6e-226
Identity = 386/445 (86.74%), Postives = 413/445 (92.81%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTG-RGEDLDF 184
           ++LEVDIISEELIKPSTPTP HLR Y LSFIDQITVHIYAP LYFYQSTDTG  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYPPLAGRPNYAS+FIDCND GVPFREA VNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V GVFKPHMEGAKIFPPK MSYD+AMT  RNRV+RRFVFKQSK+EAIR KY E+Q MINQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 365 N-QPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYF 424
           N QPSR+ESLTAF YSRFLA FKHDS I NDMS+L+NYTVNLRPKMNPPLPHDAFGNYYF
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKHDSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300

Query: 425 NLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNG 484
           N+MIFPS E LNDDENCYGLVKQLREETNKI+GEMAKKFLNED++LMKTV E+ASKVV+G
Sbjct: 301 NVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVVSG 360

Query: 485 EIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQH 544
           EI+SCAFSS CRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSKD + IDAIVHLE+++
Sbjct: 361 EIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEERY 420

Query: 545 MNKLEADELFLKYATPIPTLSSPLA 568
           MNKLE DE+F+KYATPIPT SS +A
Sbjct: 421 MNKLEGDEVFMKYATPIPTPSSRVA 445

BLAST of ClCG08G017430 vs. ExPASy TrEMBL
Match: A0A1S3BD01 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 6.9e-219
Identity = 373/424 (87.97%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTG-RGEDLDF 184
           ++LEVDIISEELIKPSTPTP HLR Y LSFIDQITVHIYAP LYFYQSTDTG  GEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 185 ALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQP 244
           ALAFKNRLR+TLPDVLSHYPPLAGRPNYAS+FIDCND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 245 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG 304
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVA+ASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 305 VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQ 364
           V GVFKPHMEGAKIFPPK MSYDTAMT  RNRV+RRFVFKQSKLEAIRAKY E+Q  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 365 NQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYFN 424
           N+PSR+ESLTAF YSRFLA FKHDS+I NDMS+L+NYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 425 LMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNGE 484
           +MIFPS E LNDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM+TV E+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 485 IISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQHM 544
           IISCAFSS CRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSKD + +DAIVHLE+++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 545 NKLE 548
           NKLE
Sbjct: 421 NKLE 424

BLAST of ClCG08G017430 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 4.8e-188
Identity = 330/439 (75.17%), Postives = 372/439 (84.74%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFA 184
           ++LE+DIISE+LIKP+TPTP HLRRYPLSFIDQITVHIYAP LYFYQ    G   D DFA
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAG---DPDFA 60

Query: 185 LAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPD 244
           + F+NRLR+TL  VLSHYPPLAGRPNY S F+DCND G  FREARVN++LADVV++A+PD
Sbjct: 61  IDFRNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPD 120

Query: 245 DLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARGV 304
           DLNRLFPVELD+FNEELMAVQFTEFACGGVA+ASCISHKIAD M LFSLNNNWAAMARGV
Sbjct: 121 DLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGV 180

Query: 305 NGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQN 364
            GVFKPHMEGAK+FP KTM+YDTA T  RNRVARRFVF+ SK+EAIR KY E+ A    +
Sbjct: 181 KGVFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AH 240

Query: 365 QPSRVESLTAFFYSRFLATFKHDSS--IPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYF 424
           +PSRVESLTAF Y RFLA  K  SS  +P+D + L+NYTVNLR KM PPLP DAFGNYYF
Sbjct: 241 RPSRVESLTAFMYGRFLAALKDGSSSQVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYF 300

Query: 425 NLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNG 484
           N MIFPS ++LN D  CYGLV +LREETNK+N +  K  LNED+ LM++V EIASK V G
Sbjct: 301 NAMIFPSPDILN-DPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKG 360

Query: 485 EIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQH 544
           E+ISCAF+S CRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSK++  IDAIVHLE+ H
Sbjct: 361 EMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPH 420

Query: 545 MNKLEADELFLKYATPIPT 562
           M KLEAD+LFL+YATP PT
Sbjct: 421 MKKLEADQLFLQYATPTPT 432

BLAST of ClCG08G017430 vs. ExPASy TrEMBL
Match: A0A6J1KPC8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 4.5e-186
Identity = 327/439 (74.49%), Postives = 369/439 (84.05%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFA 184
           ++LE+DIISE+LIKP+TPTP HLR YPLSFIDQITVHIYAP LYFY+ T  G   D DFA
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG---DPDFA 60

Query: 185 LAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPD 244
           + F+NRLR+TL  VLSHYPPLAGRPNY S F+DCND G  FREARVN++LADVV++AQPD
Sbjct: 61  IHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPD 120

Query: 245 DLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARGV 304
           DLNRLFPVELD+FNEELMAVQFTEFACGGVA+ASCISHKIAD M LFSLNNNWAAMARGV
Sbjct: 121 DLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGV 180

Query: 305 NGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAMINQN 364
            GVFKPHMEGAK+FP KT  YDTA T  RNRVARRFVF+ SK+EAIR KY E+ A    +
Sbjct: 181 KGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AH 240

Query: 365 QPSRVESLTAFFYSRFLATFKHDSS--IPNDMSYLINYTVNLRPKMNPPLPHDAFGNYYF 424
           +PSRVESLTAF Y RFLA  K  SS  +P+D + L+NYTVNLRPKM PPLP DAFGNYYF
Sbjct: 241 RPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYF 300

Query: 425 NLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVVNG 484
           N MIFPS ++LN D  C+GLV +LREETNK+N +  K  LNED+ LM++V EIASK V G
Sbjct: 301 NAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKG 360

Query: 485 EIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQQH 544
           E+ISCAF+S CRFPLYDVDFGWGRPVWVTFPALWFKNLVT LDSK+   IDAIVHLE+ H
Sbjct: 361 EMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPH 420

Query: 545 MNKLEADELFLKYATPIPT 562
           M +LEAD+LFL+YATP PT
Sbjct: 421 MKRLEADQLFLEYATPTPT 432

BLAST of ClCG08G017430 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 286.6 bits (732), Expect = 4.4e-77
Identity = 157/442 (35.52%), Postives = 263/442 (59.50%), Query Frame = 0

Query: 127 LEVDIISEELIKPSTPTPSHLR-RYPLSFIDQITVHIYAPVLYFYQS-TDTGRGEDLDFA 186
           LE+ + S+EL+KPS    +H    + LSF+DQ+   I+ P L+FY + T+    E  D  
Sbjct: 3   LEITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERSD-- 62

Query: 187 LAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPD 246
                 ++++L ++L+ Y PLAGR   + + + CND GV F EA+ +  ++ +++   P+
Sbjct: 63  -----HIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPN 122

Query: 247 DLNRLFPVELDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAMARG- 306
           +LN+L P E    ++  + VQ T F CGG+A+   +SHK+ D ++     N+WAA ARG 
Sbjct: 123 ELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQ 182

Query: 307 VNGVFKPHMEGAKIFPP---KTMSYDTAMTTGRNRVARRFVFKQSKLEAIRAKYAESQAM 366
            + +  P  + AK+FPP   + ++  T +T   N V RRFVF +S +E++R +++ ++ +
Sbjct: 183 TDEIITPSFDLAKMFPPCDIENLNMATGITK-ENIVTRRFVFLRSSVESLRERFSGNKKI 242

Query: 367 INQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDAFGNY 426
               + +RVE L+ F +SRF+A+  HD        Y + + VNLR + +P +P + FGN 
Sbjct: 243 ----RATRVEVLSVFIWSRFMASTNHDDK--TGKIYTLIHPVNLRRQADPDIPDNMFGNI 302

Query: 427 YFNLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTVTEIASKVV 486
               +  P   +  +DE    LV Q+REE  KI+    KK   ++   ++ + + AS  V
Sbjct: 303 MRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFV 362

Query: 487 NGEIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAIDAIVHLEQ 546
           NGEI+S +F+S C+FP+Y+ DFGWG+P+WV    + +KNLV F+D+K+ D I+A ++L+Q
Sbjct: 363 NGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQ 422

Query: 547 QHMNKLEADELFLKYATPIPTL 563
             M++ EADE  L+Y +  P++
Sbjct: 423 NDMSRFEADEELLRYVSSNPSV 430

BLAST of ClCG08G017430 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 240.7 bits (613), Expect = 2.8e-63
Identity = 146/452 (32.30%), Postives = 249/452 (55.09%), Query Frame = 0

Query: 127 LEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFALA 186
           + VD++S ++IKPS+PTP+HL+++ LS ++Q+   I+ P+++FY + ++ +  +      
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQ----- 60

Query: 187 FKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQFAQPDDL 246
               L+ +L + L+H+ PLAGR    +  IDCND+G  F EARVNS L++++     D L
Sbjct: 61  -LQMLKKSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSL 120

Query: 247 NRLFPVELDRF--NEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAAM-ARG 306
            +L P  +D       L+  Q + F CG ++I  CISHK+AD  ++     +WAA+ +RG
Sbjct: 121 QQLIPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRG 180

Query: 307 -VNGVFKPHMEGAKIFPPKTMSYDTAMTTGRNRV------ARRFVFKQSKLEAIRAKYAE 366
            +  +  P  +  KIFPP   S  +        +      ++RF+F  S ++A++AK + 
Sbjct: 181 SIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASS 240

Query: 367 SQAMINQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPHDA 426
            +     NQP+RVE+++A  +   +   +  S   +  S L N +V+LR +++PP   ++
Sbjct: 241 FEV----NQPTRVEAVSALIWKSAMKATRTVSG-TSKPSILAN-SVSLRSRVSPPFTKNS 300

Query: 427 FGNYYFNLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFL---NEDEDLMKTVT 486
            GN   +     ++E +N  +    LV ++R+   +       K +   N  E +     
Sbjct: 301 IGN-LVSYFAAKAEEGINQTK-LQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQK 360

Query: 487 EIASKVVNGEIISCAFSSTCRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKDEDAID 546
           E    + +G+     FSS CRF LY+ DFGWG+PVWV FP++  KN+VT LD+K+   I+
Sbjct: 361 EAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIE 420

Query: 547 AIVHLEQQHMNKLEADELFLKYATPIPTLSSP 566
           A V+L +Q MN  E D   L++A+  P++  P
Sbjct: 421 AWVNLNEQEMNLFEQDRELLQFASLNPSVIQP 437

BLAST of ClCG08G017430 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 203.0 bits (515), Expect = 6.4e-52
Identity = 148/451 (32.82%), Postives = 227/451 (50.33%), Query Frame = 0

Query: 127 LEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFALA 186
           ++V+ I++E+IKPS+ TP+ L+   LS  D I   +Y     FY        +DL     
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTK------DDLISPEQ 63

Query: 187 FKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNS-QLADVVQFAQPDD 246
             ++L+T+L + L+ + PLAGR    +  IDCND G  F +ARVN+  L+D ++      
Sbjct: 64  SSHKLKTSLAETLTKFYPLAGRIKGVT--IDCNDEGAVFVDARVNNYPLSDFLRSPDFKT 123

Query: 247 LNRLFPVEL--DRFNE----ELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNNWAA 306
           L +L P+++  D +       L+ V+ T F CGG+AI  CI+HKIAD  ++ +   +WAA
Sbjct: 124 LQQLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 183

Query: 307 MARGVNG--VFKPHMEGAKIFPPKTMSYDTAMTTGRNRVA---RRFVFKQSKLEAIRAKY 366
           MARG  G  V  P    A  +PP    +   +    N+++   +RFVF  SKLE +R K 
Sbjct: 184 MARGEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKA 243

Query: 367 AESQAMINQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNPPLPH 426
           A    +    +P RVES+TA  +  F+A    +++   DM  LI  + NLRPK+   L  
Sbjct: 244 ASEDFV---GRPKRVESVTALLWKAFVAAASSNTN-ACDMKVLIQPS-NLRPKIPSLLAE 303

Query: 427 DAFGNYYFNLMI--FPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMKTV 486
              GN  F+ ++     QE +  ++    L KQ  +  N I  E           L   +
Sbjct: 304 SLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLM 363

Query: 487 TEIASKVVNGEIISCAFSSTCRFPLYDVDFGWGRPVWVT-FPALWFKNLVTFLDSKDEDA 546
               SK+          SS C+ PLY+  FGWG PVW+    A   +N+   +DSKD   
Sbjct: 364 LTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQG 423

Query: 547 IDAIVHLEQQHMNKLEADELFLKYATPIPTL 563
           I+A V L +++M  LE +   L +A+  P++
Sbjct: 424 IEAFVTLPEENMLSLEQNTELLAFASVNPSV 441

BLAST of ClCG08G017430 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 199.9 bits (507), Expect = 5.5e-51
Identity = 145/468 (30.98%), Postives = 233/468 (49.79%), Query Frame = 0

Query: 122 MKRLELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDL 181
           M+ + ++V+ IS+E+IKPS+PTP++L+   LS  D I   +Y     FY   D    E  
Sbjct: 1   METMTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHT 60

Query: 182 DFALAFKNRLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNS-QLADVVQF 241
                  ++L+T+L + L+ + PLAGR    +  +DC D G  F +ARVN+  L + ++ 
Sbjct: 61  ------SHKLKTSLSETLTKFYPLAGRITGVT--VDCTDEGAIFVDARVNNCPLTEFLKC 120

Query: 242 AQPDDLNRLFPVE------LDRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLN 301
              D L +L P++      +      L+ V+ T F CGG+AI  CI+HKIAD  ++ +  
Sbjct: 121 PDFDALQQLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFI 180

Query: 302 NNWAAMARGVN---GVFKPHMEGAKIFPPKTMSYD---TAMTTGRNRVARRFVFKQSKLE 361
            +WAA ARG N    +  P   GA  +PP   ++          R+ + +RFVF+ SK+E
Sbjct: 181 RSWAATARGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVE 240

Query: 362 AIRAKYAESQAMINQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKM 421
            +R K A  + +   +QP+RVES+TA  +  F+A+ K  +    D   L+    NLR K+
Sbjct: 241 DLRTKAASEETV---DQPTRVESVTALIWKCFVASSKTTTC---DHKVLVQ-LANLRSKI 300

Query: 422 NPPLPHDAFGNYYFNLMIFPSQEMLNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDL 481
              L   + GN  F+ ++         +      V+ LR++  ++   +  +  + D   
Sbjct: 301 PSLLQESSIGNLMFSSVVLSIGR--GGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSS 360

Query: 482 MKTVTEIASKVVNGEIISCA-----------FSSTCRFPLYDVDFGWGRPVWV---TFPA 541
           M     I SK+ N  + + +            SS C+ PLY+  FGW  PVWV     P 
Sbjct: 361 M-----IGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPV 420

Query: 542 LWFKNLVTFLDSKDEDAIDAIVHLEQQHMNKLEADELFLKYATPIPTL 563
           L   NL   +DSKD   I+A V L +++M+  E +   L +AT  P++
Sbjct: 421 L--GNLAMLIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATMNPSV 444

BLAST of ClCG08G017430 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 190.7 bits (483), Expect = 3.3e-48
Identity = 138/453 (30.46%), Postives = 226/453 (49.89%), Query Frame = 0

Query: 125 LELEVDIISEELIKPSTPTPSHLRRYPLSFIDQITVHIYAPVLYFYQSTDTGRGEDLDFA 184
           +E  V+I+S E++KPS+PTP   R   LS +D ++  +Y   L FY +       D    
Sbjct: 1   MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAA-------DPQNL 60

Query: 185 LAFKN-----RLRTTLPDVLSHYPPLAGRPNYASNFIDCNDAGVPFREARVNSQLADVVQ 244
           L F       +L+ +L   L  + PLAGR     +F++CND G  F EARV+  L++ ++
Sbjct: 61  LGFSTEETSLKLKKSLSKTLPIFYPLAGR--IIGSFVECNDEGAVFIEARVDHLLSEFLK 120

Query: 245 FAQPDDLNRLFPVEL---DRFNEELMAVQFTEFACGGVAIASCISHKIADGMALFSLNNN 304
              P+ L  L PVE    +     ++ +Q   F+CGG+ I  C+SHKI D  +L      
Sbjct: 121 CPVPESLELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRG 180

Query: 305 WAAMARGVNGVFKPHMEGAKIFP------PKTMSYDTAMTTGRNRVARRFVFKQSKLEAI 364
           WA  +RG+     P    +++FP      P             + V +RFVF  SK++ +
Sbjct: 181 WAESSRGLGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKL 240

Query: 365 RAKYAESQAMINQNQPSRVESLTAFFYSRFLATFKHDSSIPNDMSYLINYTVNLRPKMNP 424
           RAK + +        P+RVE++TA F+       +  S  P   + ++   VNLR K++ 
Sbjct: 241 RAKASRNLV----KNPTRVEAVTALFWRCVTKVSRLSSLTPR--TSVLQILVNLRGKVD- 300

Query: 425 PLPHDAFGNYYFNLMIFPSQEMLNDDENCYGLVKQLR--EETNKINGEMAKKFLNEDEDL 484
            L  +  GN   +LMI  ++E     E    +V ++R  +E   +N +   K  +   +L
Sbjct: 301 SLCENTIGN-MLSLMILKNEEAA--IERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFEL 360

Query: 485 MKTVTEIASKVVNGEIISCAFSSTCRFPLYDVDFGWGRPVWVTFPAL-WFKNLVTFLDSK 544
           ++ + ++  +    E+     +S C+  LYD DFGWG+PVWVT      FKNL+  +D+K
Sbjct: 361 LEEIGKVYGR--GNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTK 420

Query: 545 DEDAIDAIVHLEQQHMNKLEADELFLKYATPIP 561
           D + I+A + L ++ M+  E D+  L+ A+  P
Sbjct: 421 DGEGIEAWITLTEEQMSLFECDQELLESASLNP 432