ClCG07G012480 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG07G012480
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionDelta 9 desaturase
LocationCG_Chr07: 28879849 .. 28881082 (+)
RNA-Seq ExpressionClCG07G012480
SyntenyClCG07G012480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCAATAAGAAAAGAGCTTGAAGATAAACCTGATATGAAGGAGCCATTTTCAGTGGTGAAGTCTAAACGAGCTTTCTTAAAGAGAAAATGGACAACTTTAGACCGACGTTCTGCTGGTGGAATTTTGTTCTTGCATGTGCTTTCTCTCTTGGCTCCATTTCACTACAATTGGGAAGCCTTTTGGGTTGCTTTTGCACTCTATTTTATTAGGGGCATATTAGGGATAACGCTCTCCTATCATAGAAATCTCGCACATAGAAGTTTTAAACTTTCCAAATGGCTCGAGTACACGTTTGCTTATCTAGGAGTGCAAGCCTTTCAGGTTTGTCATCTCTTAGTATTATATGTTTATTAATTAAAGTCATTATTGGAGTGATTAAAGTTTGGTAATCCTTTTATGATTTCTTTTATTGTTGTTGTTGTTTTGCTACAGGGTGATCCAATTGATTGGGTTAGTACACATAGATATCACCATCAGTTTGTTGATACGGATCGTGATCCACATAGCCCCTTAAAAGGATTTTGGTTTAGTCACATGACTTGGATGTTCGACTCGTATGGTTTGACCGAGAAGATTTACCAGAAAAATCTTGAAGATTTTGAAGAAAGAGAAGCAAAACGAAAAATTTCTCGGAATTGGTTCGTGGGTTTTCTAAAGTATGGAAGACCAAAGAATGTTGAGGATTTGCAGAAACAAGCTTTCTATAGGTTCCTTCGAAAAACATACCTTCTTCATCCCATAGCTCTTGCAATTGTTCTCTATAAATTTGGAGGACTTCCCTTTGTTGTTTGGGGAATGGTAAGATTTAACATGGAAAAAAAACTTCTATTTGTCTTCATTTTTTAACATTTTAGTTTCTACCTGCCTTATCTATCATGGAAAAATACTTACTACATCTCGTCAACACCTATTTAATTTTGTGTATCGTCCCATCAAATTTTATTTTTATATATTAAGGCATGNGACCATGCCTTTAAATTAAACCTATCATTTTCTTTATTACTAATTCTTTTTGGTAAGGATTAACATATTGGTTTTAATTGTTGGTTTTGACAATTAGCCATTTAGAAAGGAGAATGTATAAGAAAATTAATGAGAGTTTGATTGTGAATGGTGATGGCAGGGAGTGAGGCTGATTTGTGTGTTGCATGCTACACTCTTCGTAAATTCAGCATGCCACATTTGGGGCTACAGAAAATGGAACACCAATGATTTGTCCAAGAACAAT

mRNA sequence

ATGGAGGCAATAAGAAAAGAGCTTGAAGATAAACCTGATATGAAGGAGCCATTTTCAGTGGTGAAGTCTAAACGAGCTTTCTTAAAGAGAAAATGGACAACTTTAGACCGACGTTCTGCTGGTGGAATTTTGTTCTTGCATGTGCTTTCTCTCTTGGCTCCATTTCACTACAATTGGGAAGCCTTTTGGGTTGCTTTTGCACTCTATTTTATTAGGGGCATATTAGGGATAACGCTCTCCTATCATAGAAATCTCGCACATAGAAGTTTTAAACTTTCCAAATGGCTCGAGTACACGTTTGCTTATCTAGGAGTGCAAGCCTTTCAGGGTGATCCAATTGATTGGGTTAGTACACATAGATATCACCATCAGTTTGTTGATACGGATCGTGATCCACATAGCCCCTTAAAAGGATTTTGGTTTAGTCACATGACTTGGATGTTCGACTCGTATGGTTTGACCGAGAAGATTTACCAGAAAAATCTTGAAGATTTTGAAGAAAGAGAAGCAAAACGAAAAATTTCTCGGAATTGGTTCGTGGGTTTTCTAAAGTATGGAAGACCAAAGAATGTTGAGGATTTGCAGAAACAAGCTTTCTATAGGTTCCTTCGAAAAACATACCTTCTTCATCCCATAGCTCTTGCAATTGTTCTCTATAAATTTGGAGGACTTCCCTTTGTTGTTTGGGGAATGGGAGTGAGGCTGATTTGTGTGTTGCATGCTACACTCTTCGTAAATTCAGCATGCCACATTTGGGGCTACAGAAAATGGAACACCAATGATTTGTCCAAGAACAAT

Coding sequence (CDS)

ATGGAGGCAATAAGAAAAGAGCTTGAAGATAAACCTGATATGAAGGAGCCATTTTCAGTGGTGAAGTCTAAACGAGCTTTCTTAAAGAGAAAATGGACAACTTTAGACCGACGTTCTGCTGGTGGAATTTTGTTCTTGCATGTGCTTTCTCTCTTGGCTCCATTTCACTACAATTGGGAAGCCTTTTGGGTTGCTTTTGCACTCTATTTTATTAGGGGCATATTAGGGATAACGCTCTCCTATCATAGAAATCTCGCACATAGAAGTTTTAAACTTTCCAAATGGCTCGAGTACACGTTTGCTTATCTAGGAGTGCAAGCCTTTCAGGGTGATCCAATTGATTGGGTTAGTACACATAGATATCACCATCAGTTTGTTGATACGGATCGTGATCCACATAGCCCCTTAAAAGGATTTTGGTTTAGTCACATGACTTGGATGTTCGACTCGTATGGTTTGACCGAGAAGATTTACCAGAAAAATCTTGAAGATTTTGAAGAAAGAGAAGCAAAACGAAAAATTTCTCGGAATTGGTTCGTGGGTTTTCTAAAGTATGGAAGACCAAAGAATGTTGAGGATTTGCAGAAACAAGCTTTCTATAGGTTCCTTCGAAAAACATACCTTCTTCATCCCATAGCTCTTGCAATTGTTCTCTATAAATTTGGAGGACTTCCCTTTGTTGTTTGGGGAATGGGAGTGAGGCTGATTTGTGTGTTGCATGCTACACTCTTCGTAAATTCAGCATGCCACATTTGGGGCTACAGAAAATGGAACACCAATGATTTGTCCAAGAACAAT

Protein sequence

MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNTNDLSKNN
Homology
BLAST of ClCG07G012480 vs. NCBI nr
Match: XP_004152341.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KGN52907.1 hypothetical protein Csa_014904 [Cucumis sativus])

HSP 1 Score: 504.2 bits (1297), Expect = 6.8e-139
Identity = 236/266 (88.72%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+EDK D K+ FS+V  KRAF  RKWTTLDRR+A GILFLH+LSLLAPFH+NW 
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWA 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFALY I G LGITLSYHRNLAHRSFKL KWLEY FAYLGVQ FQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQFVDTDRDPHSP++GFWFSHMTWMFDSYGLTEKIY KNLEDF+EREAKR +SRNWFV
Sbjct: 121 YHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKQ FYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
           A LFVNSACHIWGYRKWNTNDLSKNN
Sbjct: 241 AALFVNSACHIWGYRKWNTNDLSKNN 266

BLAST of ClCG07G012480 vs. NCBI nr
Match: XP_022955953.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata] >KAG6581939.1 Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496.9 bits (1278), Expect = 1.1e-136
Identity = 231/266 (86.84%), Postives = 243/266 (91.35%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+EDK D K+PFSVV  KR FL RKWTTLDRR+A GILFLHVL L APFHYNW 
Sbjct: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LYF  G+LGITLSYHRNLAHRSFKL KWLEY+FAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQFVDT+RDPHSPL+GFWFSH+TWMFDSYGLT KIY K LEDFEER+AKR +SRNWFV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+ FYRFLRKTYLLHPIALA+VLY FGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
           A  FVNSACHIWGYRKWNTNDLSKNN
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNN 266

BLAST of ClCG07G012480 vs. NCBI nr
Match: XP_023528301.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 495.7 bits (1275), Expect = 2.4e-136
Identity = 230/266 (86.47%), Postives = 243/266 (91.35%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+EDK D K+PFS+V  KR FL RKWTTLDRR+A GILFLHVL L APFHYNW 
Sbjct: 1   MEATRKEVEDKYDKKKPFSMVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LYF  G+LGITLSYHRNLAHRSFKL KWLEY+FAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQFVDT+RDPHSPL+GFWFSH+TWMFDSYGLT KIY K +EDFEER+AKR +SRNWFV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYVEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKQ FYRFLRKTYLLHPIALA+VLY FGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
           A  FVNSACHIWGYRKWNTNDLSKNN
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNN 266

BLAST of ClCG07G012480 vs. NCBI nr
Match: XP_022153467.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 454.5 bits (1168), Expect = 6.2e-124
Identity = 212/266 (79.70%), Postives = 234/266 (87.97%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+ED+ D K  FSVVK +R  LKRKWT LDRRSAG ILFLH+LSLLAPFH NW 
Sbjct: 1   MEATRKEVEDQKDKKVAFSVVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWA 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFALYFI G+ GITLSYHRNLAH+SFKL KWLEY FAYLGV AFQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHH+FVDT+RDPHSPL+GFWFSH+ WMFD+Y LTEK+  KN++DF+ER+AKRK   N FV
Sbjct: 121 YHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GF+KYGRP NVEDLQKQAFYRFLRKTY+LHP ALAIVLY  GGLPF+VWGMGVRL+ VLH
Sbjct: 181 GFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
            T FVNSACHIWGYR+W+TNDLSKNN
Sbjct: 241 ITFFVNSACHIWGYRRWDTNDLSKNN 266

BLAST of ClCG07G012480 vs. NCBI nr
Match: XP_022153442.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 439.9 bits (1130), Expect = 1.6e-119
Identity = 208/270 (77.04%), Postives = 233/270 (86.30%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKE-PFS---VVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFH 60
           MEA RKELEDK + K+  FS   V+K +R FLKRKWT LDRRSAGGILFLH+L LLAPFH
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIVLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFH 60

Query: 61  YNWEAFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWV 120
            NW AFWVAFALYFI G+ GITLSYHRNLAH+SFKL KWLEY FAYLG+ AFQGDPIDWV
Sbjct: 61  CNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWV 120

Query: 121 STHRYHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISR 180
           STHRYHHQFVDT+RDPHSPL+GFWFSH+TWMFD+Y LT+K+  KN+EDFEE++AKRK   
Sbjct: 121 STHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFS 180

Query: 181 NWFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLI 240
           N FV  +KYGRP NV+DLQKQAFYRFLR+TY+LHP ALA+VLY  GGLPFVVWGMGVRL+
Sbjct: 181 NIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLV 240

Query: 241 CVLHATLFVNSACHIWGYRKWNTNDLSKNN 267
             LH T F+NSACHIWGYR+WNT DLSKNN
Sbjct: 241 WFLHITFFINSACHIWGYRRWNTTDLSKNN 270

BLAST of ClCG07G012480 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 305.1 bits (780), Expect = 8.0e-82
Identity = 140/245 (57.14%), Postives = 170/245 (69.39%), Query Frame = 0

Query: 22  KSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITLSY 81
           K K  + +R+W  +D  +   +L +H+LSLLAPF +NW A  VAF LY + G+LGITLS+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 82  HRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGFWF 141
           HRNL+H++FKL KWLEY FAY G QA QG+PIDWVSTHRYHHQF D+DRDPHSPL GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 142 SHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAFYR 201
           SHM WMFD+  +T++                             G P NV DL+KQ FYR
Sbjct: 208 SHMNWMFDTNTITQRC----------------------------GEPNNVGDLEKQPFYR 267

Query: 202 FLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNTND 261
           FLR TY+LHP+ALA+ LY  GG PF+VWGMGVR++ V H T  VNSACH+WG + WNT D
Sbjct: 268 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 304

Query: 262 LSKNN 267
           LSKNN
Sbjct: 328 LSKNN 304

BLAST of ClCG07G012480 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.6e-69
Identity = 131/265 (49.43%), Postives = 164/265 (61.89%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           + A  KE  +K    +   + + KRA  +RKW  LD   A   LF+H L LLAPF++ W 
Sbjct: 3   LSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWP 62

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           A  VA  +Y + G LGIT+SYHRNLAHRSFK+ KWLEY FAY G+ A QGDPIDWVSTHR
Sbjct: 63  ALRVALIVYTVGG-LGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHR 122

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQF D+DRDPHSP +GFWFSH+ W+FD+  L EK                        
Sbjct: 123 YHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------- 182

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
                GR  NVEDL++Q +Y+FL++T L H +    +LY FGGL F+ WGMG+ +    H
Sbjct: 183 -----GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHH 238

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKN 266
            T  +NS CH+WG R W TND S+N
Sbjct: 243 VTCLINSLCHVWGSRTWKTNDTSRN 238

BLAST of ClCG07G012480 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 264.2 bits (674), Expect = 1.6e-69
Identity = 129/247 (52.23%), Postives = 161/247 (65.18%), Query Frame = 0

Query: 20  VVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITL 79
           VV++KR+F +R WT+ D       +  H+LSLLAPF+++WEAFWV   L FI GI  ITL
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 80  SYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGF 139
           SYHRNL+HRSF L KWLEY FAY GV AFQGDPI+WVS HRYHH+  +T RDPHSP +GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 140 WFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAF 199
           WFSHM W+FD+  + E                              G  +NV+DL +Q F
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPF 258

Query: 200 YRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNT 259
           YRFL++T LLH +A + + Y  GG+P +VWG+G+ +   LH T  VNS CHIWG R WNT
Sbjct: 259 YRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNT 296

Query: 260 NDLSKNN 267
           +D SKNN
Sbjct: 319 SDFSKNN 296

BLAST of ClCG07G012480 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 9.4e-67
Identity = 124/244 (50.82%), Postives = 148/244 (60.66%), Query Frame = 0

Query: 22  KSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITLSY 81
           K +  F  R+W  LD         +H L+LLAPF++ W A WV F  Y I G LGIT+SY
Sbjct: 26  KRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGG-LGITVSY 85

Query: 82  HRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGFWF 141
           HRNLAHRSFK+ KWLEY  AY  + A QGDPIDWVSTHRYHHQF D++RDPHSP +GFWF
Sbjct: 86  HRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWF 145

Query: 142 SHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAFYR 201
           SH+ W++DS  L  K                             GR  NVEDL++Q FYR
Sbjct: 146 SHLLWIYDSAYLVSKC----------------------------GRRANVEDLKRQWFYR 205

Query: 202 FLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNTND 261
           FL+KT L H + L   L+  GG+ FV WGMGV     +H T  +NS CHIWG R W TND
Sbjct: 206 FLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTND 240

Query: 262 LSKN 266
            S+N
Sbjct: 266 TSRN 240

BLAST of ClCG07G012480 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 9.4e-67
Identity = 121/246 (49.19%), Postives = 156/246 (63.41%), Query Frame = 0

Query: 22  KSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALY-FIRGILGITLS 81
           +  + F +RKW   D ++A  +L LH L+ +APF+++W+AFW++F L  F  G+LGITL 
Sbjct: 71  RKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLC 130

Query: 82  YHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGFW 141
           +HR L H  FKL K +EY FAY G  A QGDP++WVS HRYHHQFVDT+RD HSP +GFW
Sbjct: 131 FHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFW 190

Query: 142 FSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAFY 201
           F H+ W+ D     EK                           + GR  NV DL+KQAFY
Sbjct: 191 FCHIGWVLDKDLFVEK---------------------------RGGRRNNVNDLKKQAFY 250

Query: 202 RFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNTN 261
           RFL+KTY+ H +AL  +LY  GG P++VWGMG RL+ + H+T  +NS CH WG R WNT 
Sbjct: 251 RFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTG 289

Query: 262 DLSKNN 267
           DLS NN
Sbjct: 311 DLSTNN 289

BLAST of ClCG07G012480 vs. ExPASy TrEMBL
Match: A0A0A0KWV4 (Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.3e-139
Identity = 236/266 (88.72%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+EDK D K+ FS+V  KRAF  RKWTTLDRR+A GILFLH+LSLLAPFH+NW 
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWA 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFALY I G LGITLSYHRNLAHRSFKL KWLEY FAYLGVQ FQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQFVDTDRDPHSP++GFWFSHMTWMFDSYGLTEKIY KNLEDF+EREAKR +SRNWFV
Sbjct: 121 YHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKQ FYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
           A LFVNSACHIWGYRKWNTNDLSKNN
Sbjct: 241 AALFVNSACHIWGYRKWNTNDLSKNN 266

BLAST of ClCG07G012480 vs. ExPASy TrEMBL
Match: A0A6J1GVF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457787 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 5.3e-137
Identity = 231/266 (86.84%), Postives = 243/266 (91.35%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+EDK D K+PFSVV  KR FL RKWTTLDRR+A GILFLHVL L APFHYNW 
Sbjct: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LYF  G+LGITLSYHRNLAHRSFKL KWLEY+FAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQFVDT+RDPHSPL+GFWFSH+TWMFDSYGLT KIY K LEDFEER+AKR +SRNWFV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+ FYRFLRKTYLLHPIALA+VLY FGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
           A  FVNSACHIWGYRKWNTNDLSKNN
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNN 266

BLAST of ClCG07G012480 vs. ExPASy TrEMBL
Match: A0A6J1DIZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020970 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.0e-124
Identity = 212/266 (79.70%), Postives = 234/266 (87.97%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           MEA RKE+ED+ D K  FSVVK +R  LKRKWT LDRRSAG ILFLH+LSLLAPFH NW 
Sbjct: 1   MEATRKEVEDQKDKKVAFSVVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWA 60

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFALYFI G+ GITLSYHRNLAH+SFKL KWLEY FAYLGV AFQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHH+FVDT+RDPHSPL+GFWFSH+ WMFD+Y LTEK+  KN++DF+ER+AKRK   N FV
Sbjct: 121 YHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFV 180

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
           GF+KYGRP NVEDLQKQAFYRFLRKTY+LHP ALAIVLY  GGLPF+VWGMGVRL+ VLH
Sbjct: 181 GFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLH 240

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKNN 267
            T FVNSACHIWGYR+W+TNDLSKNN
Sbjct: 241 ITFFVNSACHIWGYRRWDTNDLSKNN 266

BLAST of ClCG07G012480 vs. ExPASy TrEMBL
Match: A0A6J1DIX0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020949 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.6e-120
Identity = 208/270 (77.04%), Postives = 233/270 (86.30%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKE-PFS---VVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFH 60
           MEA RKELEDK + K+  FS   V+K +R FLKRKWT LDRRSAGGILFLH+L LLAPFH
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIVLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFH 60

Query: 61  YNWEAFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWV 120
            NW AFWVAFALYFI G+ GITLSYHRNLAH+SFKL KWLEY FAYLG+ AFQGDPIDWV
Sbjct: 61  CNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWV 120

Query: 121 STHRYHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISR 180
           STHRYHHQFVDT+RDPHSPL+GFWFSH+TWMFD+Y LT+K+  KN+EDFEE++AKRK   
Sbjct: 121 STHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFS 180

Query: 181 NWFVGFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLI 240
           N FV  +KYGRP NV+DLQKQAFYRFLR+TY+LHP ALA+VLY  GGLPFVVWGMGVRL+
Sbjct: 181 NIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLV 240

Query: 241 CVLHATLFVNSACHIWGYRKWNTNDLSKNN 267
             LH T F+NSACHIWGYR+WNT DLSKNN
Sbjct: 241 WFLHITFFINSACHIWGYRRWNTTDLSKNN 270

BLAST of ClCG07G012480 vs. ExPASy TrEMBL
Match: A0A6J1GCE1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452892 PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 5.5e-118
Identity = 201/252 (79.76%), Postives = 217/252 (86.11%), Query Frame = 0

Query: 15  KEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGI 74
           K   S+VKSK A L RKWT LDRRSAGG+LFLH L LLAPFH NW AFWVAFALYFI G+
Sbjct: 6   KVALSMVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGL 65

Query: 75  LGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHS 134
            G+TLSYHRNLAH+SFKL KWLEY FAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHS
Sbjct: 66  FGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHS 125

Query: 135 PLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDL 194
           PL GFWFSH+ WMFD+Y LT+K+Y KN++DFEER  KRK  RN  VG++KYGRP NVEDL
Sbjct: 126 PLDGFWFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDL 185

Query: 195 QKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGY 254
           QKQ FYRFLRKTY+LHP A AIVLY  GGLPFVVWGMGVRL+ VLH T FVNSACHIWGY
Sbjct: 186 QKQGFYRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGY 245

Query: 255 RKWNTNDLSKNN 267
           R W+TNDLSKNN
Sbjct: 246 RLWDTNDLSKNN 257

BLAST of ClCG07G012480 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 305.1 bits (780), Expect = 5.7e-83
Identity = 140/245 (57.14%), Postives = 170/245 (69.39%), Query Frame = 0

Query: 22  KSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITLSY 81
           K K  + +R+W  +D  +   +L +H+LSLLAPF +NW A  VAF LY + G+LGITLS+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 82  HRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGFWF 141
           HRNL+H++FKL KWLEY FAY G QA QG+PIDWVSTHRYHHQF D+DRDPHSPL GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 142 SHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAFYR 201
           SHM WMFD+  +T++                             G P NV DL+KQ FYR
Sbjct: 208 SHMNWMFDTNTITQRC----------------------------GEPNNVGDLEKQPFYR 267

Query: 202 FLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNTND 261
           FLR TY+LHP+ALA+ LY  GG PF+VWGMGVR++ V H T  VNSACH+WG + WNT D
Sbjct: 268 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 304

Query: 262 LSKNN 267
           LSKNN
Sbjct: 328 LSKNN 304

BLAST of ClCG07G012480 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-70
Identity = 131/265 (49.43%), Postives = 164/265 (61.89%), Query Frame = 0

Query: 1   MEAIRKELEDKPDMKEPFSVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWE 60
           + A  KE  +K    +   + + KRA  +RKW  LD   A   LF+H L LLAPF++ W 
Sbjct: 3   LSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWP 62

Query: 61  AFWVAFALYFIRGILGITLSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHR 120
           A  VA  +Y + G LGIT+SYHRNLAHRSFK+ KWLEY FAY G+ A QGDPIDWVSTHR
Sbjct: 63  ALRVALIVYTVGG-LGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHR 122

Query: 121 YHHQFVDTDRDPHSPLKGFWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFV 180
           YHHQF D+DRDPHSP +GFWFSH+ W+FD+  L EK                        
Sbjct: 123 YHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------- 182

Query: 181 GFLKYGRPKNVEDLQKQAFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240
                GR  NVEDL++Q +Y+FL++T L H +    +LY FGGL F+ WGMG+ +    H
Sbjct: 183 -----GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHH 238

Query: 241 ATLFVNSACHIWGYRKWNTNDLSKN 266
            T  +NS CH+WG R W TND S+N
Sbjct: 243 VTCLINSLCHVWGSRTWKTNDTSRN 238

BLAST of ClCG07G012480 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-70
Identity = 129/247 (52.23%), Postives = 161/247 (65.18%), Query Frame = 0

Query: 20  VVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITL 79
           VV++KR+F +R WT+ D       +  H+LSLLAPF+++WEAFWV   L FI GI  ITL
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 80  SYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGF 139
           SYHRNL+HRSF L KWLEY FAY GV AFQGDPI+WVS HRYHH+  +T RDPHSP +GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 140 WFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAF 199
           WFSHM W+FD+  + E                              G  +NV+DL +Q F
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPF 258

Query: 200 YRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNT 259
           YRFL++T LLH +A + + Y  GG+P +VWG+G+ +   LH T  VNS CHIWG R WNT
Sbjct: 259 YRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNT 296

Query: 260 NDLSKNN 267
           +D SKNN
Sbjct: 319 SDFSKNN 296

BLAST of ClCG07G012480 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 255.0 bits (650), Expect = 6.7e-68
Identity = 124/244 (50.82%), Postives = 148/244 (60.66%), Query Frame = 0

Query: 22  KSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGITLSY 81
           K +  F  R+W  LD         +H L+LLAPF++ W A WV F  Y I G LGIT+SY
Sbjct: 26  KRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGG-LGITVSY 85

Query: 82  HRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKGFWF 141
           HRNLAHRSFK+ KWLEY  AY  + A QGDPIDWVSTHRYHHQF D++RDPHSP +GFWF
Sbjct: 86  HRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWF 145

Query: 142 SHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQAFYR 201
           SH+ W++DS  L  K                             GR  NVEDL++Q FYR
Sbjct: 146 SHLLWIYDSAYLVSKC----------------------------GRRANVEDLKRQWFYR 205

Query: 202 FLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWNTND 261
           FL+KT L H + L   L+  GG+ FV WGMGV     +H T  +NS CHIWG R W TND
Sbjct: 206 FLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTND 240

Query: 262 LSKN 266
            S+N
Sbjct: 266 TSRN 240

BLAST of ClCG07G012480 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.1e-65
Identity = 126/247 (51.01%), Postives = 154/247 (62.35%), Query Frame = 0

Query: 19  SVVKSKRAFLKRKWTTLDRRSAGGILFLHVLSLLAPFHYNWEAFWVAFALYFIRGILGIT 78
           +V K KRAFL RKWT +D      +  +H+L LLAPF+Y WEAF  A A+  I   L IT
Sbjct: 15  AVRKEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFA-AMVGISTNLSIT 74

Query: 79  LSYHRNLAHRSFKLSKWLEYTFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLKG 138
            SYHRNL HRSFKL KWLEY FAY  + A QG PIDWVSTHR+HHQF D+DRDPHSP++G
Sbjct: 75  FSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEG 134

Query: 139 FWFSHMTWMFDSYGLTEKIYQKNLEDFEEREAKRKISRNWFVGFLKYGRPKNVEDLQKQA 198
           FWFSH+ W+FD+  + EK                             G   NV DL++Q 
Sbjct: 135 FWFSHVFWIFDTSYIREKC----------------------------GGRDNVMDLKQQW 194

Query: 199 FYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHATLFVNSACHIWGYRKWN 258
           FYRFL+ T  LH +   I++Y +GGLP++ W +GV      HAT  +NSACHIWG R WN
Sbjct: 195 FYRFLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWN 232

Query: 259 TNDLSKN 266
           T D S+N
Sbjct: 255 TKDTSRN 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004152341.26.8e-13988.72palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_022955953.11.1e-13686.84palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023528301.12.4e-13686.47palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022153467.16.2e-12479.70palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022153442.11.6e-11977.04palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
Match NameE-valueIdentityDescription
Q949X08.0e-8257.14Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657971.6e-6949.43Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9LVZ31.6e-6952.23Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SID29.4e-6750.82Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
Q9FV689.4e-6749.19Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KWV43.3e-13988.72Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1[more]
A0A6J1GVF35.3e-13786.84palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIZ73.0e-12479.70palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIX07.6e-12077.04palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GCE15.5e-11879.76palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.15.7e-8357.14fatty acid desaturase 5 [more]
AT1G06080.11.1e-7049.43delta 9 desaturase 1 [more]
AT3G15870.11.1e-7052.23Fatty acid desaturase family protein [more]
AT2G31360.16.7e-6850.8216:0delta9 desaturase 2 [more]
AT1G06120.11.1e-6551.01Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 82..102
score: 33.08
coord: 59..81
score: 24.03
coord: 119..148
score: 52.28
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 184..266
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 24..167
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 58..266
e-value: 1.11114E-45
score: 149.628
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 184..266
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 24..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG07G012480.1ClCG07G012480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water