ClCG06G000850 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG06G000850
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	PMEI domain-containing protein
Location	CG_Chr06: 831262 .. 831828 (-)
RNA-Seq Expression	ClCG06G000850
Synteny	ClCG06G000850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATATCTCATTATCTCTGTGTTTTCATTCTCTCCATTTTCTTCTTCTCCAATTTCCTCATTACACAATCCTCCAACAACATCAAAACATCTGATCTAATCCACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACATTTCCTTCAACTTTTGCTTAACATCTCTAGAATTTGCCACCAAGCATAGCCGATGCACCGATGTTCGTCGCCTCGGCCTCCTCTCGATTGGGCTCCTACATAGGAATGTGACCAGCACCCGCCACCATATCGTAAAGTCGATGAGAAACAAGAAGCTTGACCCGTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATTCCGATGCGATCCCGACGGTGAAGCAGGCGATGAGGGATTATAGGTTGAAACGATACGACGATGCGAACATCGCGATTAGTTCCGTGATGGATGCAGCAACGACATGTGAGGATGGGTTTAAGGAGAGGGAAGGGGTGGCTTCACCATTGAAGAAGAGGGATGAAGATGCTTTTGAATTGGGAGCCATTACTCTATCCATTATGAGTTTGCTTCGTTAA

mRNA sequence

ATGGCCATATCTCATTATCTCTGTGTTTTCATTCTCTCCATTTTCTTCTTCTCCAATTTCCTCATTACACAATCCTCCAACAACATCAAAACATCTGATCTAATCCACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACATTTCCTTCAACTTTTGCTTAACATCTCTAGAATTTGCCACCAAGCATAGCCGATGCACCGATGTTCGTCGCCTCGGCCTCCTCTCGATTGGGCTCCTACATAGGAATGTGACCAGCACCCGCCACCATATCGTAAAGTCGATGAGAAACAAGAAGCTTGACCCGTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATTCCGATGCGATCCCGACGGTGAAGCAGGCGATGAGGGATTATAGGTTGAAACGATACGACGATGCGAACATCGCGATTAGTTCCGTGATGGATGCAGCAACGACATGTGAGGATGGGTTTAAGGAGAGGGAAGGGGTGGCTTCACCATTGAAGAAGAGGGATGAAGATGCTTTTGAATTGGGAGCCATTACTCTATCCATTATGAGTTTGCTTCGTTAA

Coding sequence (CDS)

ATGGCCATATCTCATTATCTCTGTGTTTTCATTCTCTCCATTTTCTTCTTCTCCAATTTCCTCATTACACAATCCTCCAACAACATCAAAACATCTGATCTAATCCACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACATTTCCTTCAACTTTTGCTTAACATCTCTAGAATTTGCCACCAAGCATAGCCGATGCACCGATGTTCGTCGCCTCGGCCTCCTCTCGATTGGGCTCCTACATAGGAATGTGACCAGCACCCGCCACCATATCGTAAAGTCGATGAGAAACAAGAAGCTTGACCCGTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATTCCGATGCGATCCCGACGGTGAAGCAGGCGATGAGGGATTATAGGTTGAAACGATACGACGATGCGAACATCGCGATTAGTTCCGTGATGGATGCAGCAACGACATGTGAGGATGGGTTTAAGGAGAGGGAAGGGGTGGCTTCACCATTGAAGAAGAGGGATGAAGATGCTTTTGAATTGGGAGCCATTACTCTATCCATTATGAGTTTGCTTCGTTAA

Protein sequence

MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEFATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLLR

Homology

[Close]

BLAST of ClCG06G000850 vs. NCBI nr
Match: KAA0039956.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24547.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 318.5 bits (815), Expect = 3.7e-83
Identity = 160/188 (85.11%), Postives = 171/188 (90.96%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAI HY  VFILSIFFFSNFLI QSS  IKT+DLI+KTCKKISREDPNISFNFCLTSL+ 
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           AT HSRCTDVR LGLLSIGLL+RNVTST HHI K ++NKKLDPFVK CLDDCLELYSDAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTVKQAMRDY+ KRYDD N+AI SVMDAATTCEDGFKER+GVASPLKKRDE+AFELGAI 
Sbjct: 121 PTVKQAMRDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLLR 189
           LSIMSL+R
Sbjct: 181 LSIMSLVR 188

[Close]

BLAST of ClCG06G000850 vs. NCBI nr
Match: XP_008460048.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 317.4 bits (812), Expect = 8.4e-83
Identity = 159/188 (84.57%), Postives = 171/188 (90.96%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAI HY  VFILSIFFFSNFLI QSS  IKT+DLI+KTCKKISREDPNISFNFCLTSL+ 
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           AT HSRCTDVR LGLLSIGLL+RNVTST HHI K ++NKKLDPFVK CLDDCLELYSDAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTVKQAM+DY+ KRYDD N+AI SVMDAATTCEDGFKER+GVASPLKKRDE+AFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLLR 189
           LSIMSL+R
Sbjct: 181 LSIMSLVR 188

[Close]

BLAST of ClCG06G000850 vs. NCBI nr
Match: XP_004145026.1 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 311.2 bits (796), Expect = 6.0e-81
Identity = 156/187 (83.42%), Postives = 167/187 (89.30%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAI HY  VFILS+FFFSNFLI QSS  IKT+DLI+KTCKKISREDPN+SFNFCL SL+ 
Sbjct: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           A  HSRCTDVR LGL SIG L RNVTST HHI K +RNKKLDPFVKLCLDDCLELY+DAI
Sbjct: 61  AANHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTVKQAM+DY+ KRYDDAN+AISSVMDAATTCEDGFKER+GVASPLKKRD DAFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180

Query: 181 LSIMSLL 188
           LSIMSLL
Sbjct: 181 LSIMSLL 187

[Close]

BLAST of ClCG06G000850 vs. NCBI nr
Match: KAE8646718.1 (hypothetical protein Csa_005009 [Cucumis sativus])

HSP 1 Score: 297.7 bits (761), Expect = 6.8e-77
Identity = 149/179 (83.24%), Postives = 160/179 (89.39%), Query Frame = 0


Query: 9   VFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEFATKHSRCT 68
           VF  +IFFFSNFLI QSS  IKT+DLI+KTCKKISREDPN+SFNFCL SL+ A  HSRCT
Sbjct: 121 VFCKAIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLAANHSRCT 180

Query: 69  DVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMR 128
           DVR LGL SIG L RNVTST HHI K +RNKKLDPFVKLCLDDCLELY+DAIPTVKQAM+
Sbjct: 181 DVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMK 240

Query: 129 DYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLL 188
           DY+ KRYDDAN+AISSVMDAATTCEDGFKER+GVASPLKKRD DAFELGAI LSIMSLL
Sbjct: 241 DYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 299

[Close]

BLAST of ClCG06G000850 vs. NCBI nr
Match: XP_038875573.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 282.3 bits (721), Expect = 3.0e-72
Identity = 144/187 (77.01%), Postives = 159/187 (85.03%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAIS Y   FILSIFFFSNFLITQSS  I T+DLI +TC KISREDPNIS++FCLTSLE 
Sbjct: 1   MAISLYFSAFILSIFFFSNFLITQSSITINTTDLIQETCMKISREDPNISYDFCLTSLES 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           ATK   C ++R LGL+SIGLL RNVTSTRHHI+  M N KL PFVK CL+DCLELYSDA+
Sbjct: 61  ATKDIECINLRHLGLISIGLLRRNVTSTRHHIISLMENTKLYPFVKFCLEDCLELYSDAV 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTV QAM DY+LK+YDDANIA+S VMDAA TCEDGFKER+GV SPLKKRDEDAFELGAI 
Sbjct: 121 PTVTQAMWDYKLKQYDDANIAVSLVMDAAETCEDGFKERKGVVSPLKKRDEDAFELGAIA 180

Query: 181 LSIMSLL 188
           LSI+SLL
Sbjct: 181 LSIISLL 187

[Close]

BLAST of ClCG06G000850 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.4e-29
Identity = 67/178 (37.64%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 10  FILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEFATKHSRCTD 69
           F L IFFF+  L+ Q+   + ++D++  TCKK+++  PN++++FC+ SL  A   S   D
Sbjct: 5   FSLCIFFFNLLLLLQA---VISADIVQGTCKKVAQRSPNVNYDFCVKSLG-ADPKSHTAD 64

Query: 70  VRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMRD 129
           ++ LG++S  L  ++ +  +  I + +++ K+DP +K  L+DC+ LY+DA  +V++A+ D
Sbjct: 65  LQGLGVISANLAIQHGSKIQTFIGRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIAD 124

Query: 130 YRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLL 188
           ++ K Y  AN+ +S+ +D + TCEDGFKE++G+ SP+ K ++D  +L AI+L+I  LL
Sbjct: 125 FKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of ClCG06G000850 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.3e-27
Identity = 60/157 (38.22%), Postives = 108/157 (68.79%), Query Frame = 0


Query: 31  TSDLIHKTCKKISREDPNISFNFCLTSLEFATKHSRCTDVRRLGLLSIGLLHRNVTSTRH 90
           ++D++  TCKK+++  PN++++FC+ SL  A   S   D++ LG++S  L  +  +  + 
Sbjct: 14  SADIVQGTCKKVAQRSPNVNYDFCVKSLG-ADPKSHSADLQGLGVISANLAIQQGSKIQT 73

Query: 91  HIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMRDYRLKRYDDANIAISSVMDAAT 150
            I + +++ K+DP +K  L+DC+ LY+DA  +V++A+ D++ K Y  AN+ +S+ +D + 
Sbjct: 74  FIGRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSV 133

Query: 151 TCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLL 188
           TCEDGFKE++G+ASP+ K ++D  +L AI+L+I  LL
Sbjct: 134 TCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

[Close]

BLAST of ClCG06G000850 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 51/179 (28.49%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 10  FILSIFFFSNFLITQSSNNIKTSD-LIHKTCKKISREDPNISFNFCLTSLEFATKHSRCT 69
           F++S+  FS FL     N   T+  LI  +CKK   +DP +S++FC+ SL    +    T
Sbjct: 3   FLVSLVIFSLFL-----NGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAAT 62

Query: 70  DVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMR 129
            +  L L S       +T+ +  + + +++++    V+  L  CL  Y+DA   +  A+ 
Sbjct: 63  TLESLVLASTKTAAAKITNLKGIVAQDLKDQRYQDIVE-DLKLCLGFYNDANDDLTTALA 122

Query: 130 DYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLL 188
           + + + Y  ANI +S+ +D    CED FKE +   SP+   +   F+   I L+  ++L
Sbjct: 123 NIKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of ClCG06G000850 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 2.4e-08
Identity = 55/189 (29.10%), Postives = 90/189 (47.62%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFL--ITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSL 60
           MA S+      +S+     F+  ++Q  +    + LI + C K    +P++    CL +L
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPK--TRNPSL----CLQAL 60

Query: 61  EFATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSD 120
           E +   S   D++ LG  SI +   +   T   I+ S+ N+  DP +K   + C E Y+D
Sbjct: 61  E-SDPRSASKDLKGLGQFSIDIAQASAKQT-SKIIASLTNQATDPKLKGRYETCSENYAD 120

Query: 121 AIPTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGA 180
           AI ++ QA +      Y+  NI  S+  D A TCED F+    + + L + D    +L  
Sbjct: 121 AIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCD 180

Query: 181 ITLSIMSLL 188
           I L I +LL
Sbjct: 181 IVLVISNLL 181

[Close]

BLAST of ClCG06G000850 vs. ExPASy TrEMBL
Match: A0A5A7TCY0 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00920 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.8e-83
Identity = 160/188 (85.11%), Postives = 171/188 (90.96%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAI HY  VFILSIFFFSNFLI QSS  IKT+DLI+KTCKKISREDPNISFNFCLTSL+ 
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           AT HSRCTDVR LGLLSIGLL+RNVTST HHI K ++NKKLDPFVK CLDDCLELYSDAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTVKQAMRDY+ KRYDD N+AI SVMDAATTCEDGFKER+GVASPLKKRDE+AFELGAI 
Sbjct: 121 PTVKQAMRDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLLR 189
           LSIMSL+R
Sbjct: 181 LSIMSLVR 188

[Close]

BLAST of ClCG06G000850 vs. ExPASy TrEMBL
Match: A0A1S3CBQ9 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498981 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 4.0e-83
Identity = 159/188 (84.57%), Postives = 171/188 (90.96%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAI HY  VFILSIFFFSNFLI QSS  IKT+DLI+KTCKKISREDPNISFNFCLTSL+ 
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           AT HSRCTDVR LGLLSIGLL+RNVTST HHI K ++NKKLDPFVK CLDDCLELYSDAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTVKQAM+DY+ KRYDD N+AI SVMDAATTCEDGFKER+GVASPLKKRDE+AFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLLR 189
           LSIMSL+R
Sbjct: 181 LSIMSLVR 188

[Close]

BLAST of ClCG06G000850 vs. ExPASy TrEMBL
Match: A0A0A0K951 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080380 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.9e-81
Identity = 156/187 (83.42%), Postives = 167/187 (89.30%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAI HY  VFILS+FFFSNFLI QSS  IKT+DLI+KTCKKISREDPN+SFNFCL SL+ 
Sbjct: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           A  HSRCTDVR LGL SIG L RNVTST HHI K +RNKKLDPFVKLCLDDCLELY+DAI
Sbjct: 61  AANHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
           PTVKQAM+DY+ KRYDDAN+AISSVMDAATTCEDGFKER+GVASPLKKRD DAFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180

Query: 181 LSIMSLL 188
           LSIMSLL
Sbjct: 181 LSIMSLL 187

[Close]

BLAST of ClCG06G000850 vs. ExPASy TrEMBL
Match: A0A6J1KH00 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494271 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.3e-62
Identity = 134/187 (71.66%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAIS +  +FILSIFFFSNFLITQS +NI T  LIHKTC KI+ +DPN+SFNFCLTSL+F
Sbjct: 1   MAISPF-SLFILSIFFFSNFLITQSFSNIDT-PLIHKTCNKIAHQDPNVSFNFCLTSLQF 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           ATKHS   D+RRLGLLS+GL+  NVT+T H I + +RNKKLD  VKLCL DCLELYSDAI
Sbjct: 61  ATKHSYYVDLRRLGLLSMGLVCSNVTNTYHFITELIRNKKLDSSVKLCLADCLELYSDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
            TVK AMR+YRLKRY + N+AIS+VM+AA TCE+GFKE++ V SPLKKRDED FELGAI 
Sbjct: 121 STVKLAMRNYRLKRYKNDNVAISAVMNAAETCENGFKEKK-VNSPLKKRDEDVFELGAIV 180

Query: 181 LSIMSLL 188
           L+IMS+L
Sbjct: 181 LAIMSML 184

[Close]

BLAST of ClCG06G000850 vs. ExPASy TrEMBL
Match: A0A6J1HN05 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464420 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.9e-62
Identity = 133/187 (71.12%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           MAIS +  +FILSIFFFSNFLIT+S +N  T  LIHKTC KI R+DPN+SFNFCLTSL+F
Sbjct: 1   MAISPF-SLFILSIFFFSNFLITRSVSNTDT-PLIHKTCNKIVRQDPNVSFNFCLTSLQF 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
           A KHS   D+RRLGLLSIGL+  NVT+T H I + +RNK LD  +KLCL DCLELYSDAI
Sbjct: 61  AIKHSYYVDLRRLGLLSIGLVCSNVTNTYHFITELIRNKNLDSSIKLCLVDCLELYSDAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAIT 180
            TVK AMR+YRL+RY+DAN+AIS+VMDAA TCE+GFKE++ V SPLKKRDED FELGAI 
Sbjct: 121 STVKLAMRNYRLRRYNDANVAISAVMDAAETCENGFKEKK-VNSPLKKRDEDVFELGAIV 180

Query: 181 LSIMSLL 188
           L+IMS+L
Sbjct: 181 LAIMSML 184

[Close]

BLAST of ClCG06G000850 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.0e-18
Identity = 51/180 (28.33%), Postives = 95/180 (52.78%), Query Frame = 0


Query: 9   VFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEFATKHSRCT 68
           +++++ F  SN L    +       LI  +CKK +   P   +N C+TSLE   +     
Sbjct: 5   LYLVTFFVLSNGLANGQT-------LIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAK 64

Query: 69  DVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMR 128
           D+  L + S        T+ +  + K ++ KK++    + L DCL+LY+DAI ++ +A+ 
Sbjct: 65  DLAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALA 124

Query: 129 DYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLLR 188
             + + Y      +S+ MD  +TCE GFKER+   SP+ K +++ +++  I L+  ++L+
Sbjct: 125 GVKSRNYPTVKTVLSAAMDTPSTCETGFKERK-APSPVTKENDNLYQMILIPLAFTNMLK 176

[Close]

BLAST of ClCG06G000850 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 5.9e-18
Identity = 54/162 (33.33%), Postives = 90/162 (55.56%), Query Frame = 0


Query: 14  IFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEFATKHSRCTDVRRL 73
           +F+F+ F +  ++  I  S LI   CKK + ++P I +NFC+ SLE   +      + RL
Sbjct: 4   LFYFAMFFLLLNAFTIAQS-LIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRL 63

Query: 74  GLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMRDYRLK 133
            +LS        TS +  + K ++  + + + +  L DCLELYSDA  ++K+A+   + +
Sbjct: 64  VMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSR 123

Query: 134 RYDDANIAISSVMDAATTCEDGFKE-REGVASPLKKRDEDAF 175
            Y  AN+ IS+ M A  +CE GFKE ++ + SP  K ++  F
Sbjct: 124 DYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of ClCG06G000850 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 8.5e-17
Identity = 61/188 (32.45%), Postives = 95/188 (50.53%), Query Frame = 0


Query: 12  LSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEFA-TKHSRCTDV 71
           LS  F+  FL    S    TSD+I +TCK  + +     +NFC++SL  +       T++
Sbjct: 3   LSQVFYIIFLFFLVSQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNL 62

Query: 72  RRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMRDY 131
             L L+ +     N T+T   I + + +     F   CL DCLELY DA   +++A+R +
Sbjct: 63  SSLALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVF 122

Query: 132 -RLKRYDDANIAISSVMDAATTCEDGFKERE-------GV---ASPLKKRDEDAFELGAI 188
              K     N+ +S+ M++A TCE+GF+ER+       GV    SP+   +   FE G I
Sbjct: 123 ITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQI 182

[Close]

BLAST of ClCG06G000850 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.0e-14
Identity = 54/188 (28.72%), Postives = 93/188 (49.47%), Query Frame = 0


Query: 1   MAISHYLCVFILSIFFFSNFLITQSSNNIKTSDLIHKTCKKISREDPNISFNFCLTSLEF 60
           M  S YL +F L +  F+               LI  +CKK + +DP + ++FC+ SLE 
Sbjct: 1   MKFSLYLVMFFLLLNCFAT-----------AQSLIRDSCKKATTKDPKLKYDFCVKSLEE 60

Query: 61  ATKHSRCTDVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAI 120
             +      +  L  +S        TS +  + K ++  K +  V+  L DCLELY++AI
Sbjct: 61  NPQSKTAKSLEGLVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAI 120

Query: 121 PTVKQAMRDYRLKRYDDANIAISSVMDAATTCEDGF-KEREGVASPLKKRDEDAFELGAI 180
            ++ Q++   + + Y  A + +S+ MDA  +CE  F K ++ V SP  K ++  F +  I
Sbjct: 121 DSLNQSLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLI 175

Query: 181 TLSIMSLL 188
            ++  S+L
Sbjct: 181 PIAFTSML 175

[Close]

BLAST of ClCG06G000850 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 8.8e-14
Identity = 52/179 (29.05%), Postives = 92/179 (51.40%), Query Frame = 0


Query: 10  FILSIFFFSNFLITQSSNNIKTSD-LIHKTCKKISREDPNISFNFCLTSLEFATKHSRCT 69
           F++S+  FS FL     N   T+  LI  +CKK   +DP  S++FC+ SL    +    T
Sbjct: 3   FLVSLVIFSLFL-----NGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAAT 62

Query: 70  DVRRLGLLSIGLLHRNVTSTRHHIVKSMRNKKLDPFVKLCLDDCLELYSDAIPTVKQAMR 129
            +  L L S   +   +T+ +  + + +++++    V+  L  CL  Y DA  ++K A+ 
Sbjct: 63  TLEDLALASTKNVAAKITNLKGIVAQDLKDQRYQDIVE-DLKLCLGFYKDANDSLKTALA 122

Query: 130 DYRLKRYDDANIAISSVMDAATTCEDGFKEREGVASPLKKRDEDAFELGAITLSIMSLL 188
           + + + YD AN  +S+ ++A   CED FKE E   SP+   +   ++   I L+  ++L
Sbjct: 123 NIKSRDYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTML 174

The following BLAST results are available for this feature:

BLAST of ClCG06G000850 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039956.1	3.7e-83	85.11	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24547.1 putative inv...	[more]
XP_008460048.1	8.4e-83	84.57	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
XP_004145026.1	6.0e-81	83.42	putative invertase inhibitor [Cucumis sativus]	[more]
KAE8646718.1	6.8e-77	83.24	hypothetical protein Csa_005009 [Cucumis sativus]	[more]
XP_038875573.1	3.0e-72	77.01	putative invertase inhibitor [Benincasa hispida]	[more]

BLAST of ClCG06G000850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	9.4e-29	37.64	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	2.3e-27	38.22	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	2.8e-12	28.49	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
P83326	2.4e-08	29.10	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]

BLAST of ClCG06G000850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TCY0	1.8e-83	85.11	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CBQ9	4.0e-83	84.57	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498981 PE=4 SV=1	[more]
A0A0A0K951	2.9e-81	83.42	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080380 PE=4 S...	[more]
A0A6J1KH00	2.3e-62	71.66	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494271 PE=4 SV...	[more]
A0A6J1HN05	3.9e-62	71.12	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464420 PE=4 ...	[more]

BLAST of ClCG06G000850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	2.0e-18	28.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	5.9e-18	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	8.5e-17	32.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	3.0e-14	28.72	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	8.8e-14	29.05	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..183 e-value: 1.8E-25 score: 100.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 9..186 e-value: 5.9E-34 score: 114.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..183 e-value: 7.6E-23 score: 81.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..187 e-value: 1.2E-32 score: 115.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..187
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 28..188
None	No IPR available	PANTHER	PTHR31080:SF226	PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATED	coord: 28..188
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 39..183 e-value: 1.43555E-39 score: 130.178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG06G000850.1	ClCG06G000850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity