ClCG05G020240 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG05G020240
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionIsoaspartyl peptidase/L-asparaginase
LocationCG_Chr05: 32432202 .. 32433640 (+)
RNA-Seq ExpressionClCG05G020240
SyntenyClCG05G020240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGAGAGAGAGAGAGAGAAACTGAAAAATGGGAGGTTGGGCCATAGCGGTTCATGGTGGGGCTGGCGTGGATCCCAATCTCCCACTTCAACGCCAAGACGATGCCAAAAAACTTCTCACTCGCTGCCTTGATCTTGGCATCCACGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTTGAACTCGTCGTACGCTTTCCCCCTTCCCCTTTTCTATTTCCCTTCTTCCTCTTCATGCATCATTATTTCATTACATGTTTTTCTTATATAACCAAACCAACAATCTCCTCTTCATTTTCAAGGTCAGAGAATTGGAAACCGATCCCCTGTTCAATTCGGGGCGTGGATCTGCCCTCACAGAGGAGGGGACCGTAGAGATGGAGGCCAGCATCATGGACGGCCCAAAACGGAGATGCGGGGCGGTATCTGGACTCACCACCGTCAAAAACCCCGTCTCCCTCGCTCGTCTTGTCATGGATAAATCTCCTCATTCTTATCTGGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGTTTCATTACATTTCCATTCTCATTTCCCTTTTTCTTGATTTCATCACTTCCATTTTCTCTGACATTTTGGATTGTCTCAGGGTGTGGAGATTGTGTGCAATGACTATTTCATCACCCAAGAAAACGTCGGACTGCTTGAACTAGCCAAAGAAGCCAACAGTATATGGGTACGCCCGCGATATAATCATTCGTTACAAATAACCGTCCGAAACGGTCACACTTTTAACATTTTCTTTTGGTTTTTGATTTGGAACAGTTGGATCATAGGGTCCCAAGCGAGACGTGTAGCGCGAGCGTGGAGACACCATTGCAAATGAACGGCTTCCCAATCAGCGTGTACGCGCCCGAGACGGTGGGGTGCGTGGTGGTTGATAGCGAGGGAAGGTGCGCCGCGGCTACATCCACTGGTGGATTGATAAACAAAAAGATTGGACGCATCGGAGACTCGCCGTTGATTGGATCAGGGACGTACGCGTGTGATGTATGTGGAGTATCTTGCACGGGAGAAGGGGAGGCGATCATAAGAGGGACTTTGGCCCGAGAAGTGGCGGCGGTGATGGAATACAAAGGGTTGGGGCTTCAAGAGGCGGTGAATTACGTGATAGAGGAAAGATTGGACGAAGGCAAGGCGGGATTGATTGCAGTGTCTAGCAATGGGGAAGTGGCGTGTGGGTTCAACACAACCGGGATGTTCAGGGGATGCGCCACGGAGGATGGGTTTATGGAGGTTGGGATTTGGTAATCTCAATAGTTACATAAATTAAGAGTGGAATCAAGTTTTATTTGGGTTTTAATGTTGTTTTAGTATGCTGTGTTCTCTTCTTGGTGAATCTTTTTTCTTCATTTTCTGAATTCAGATGCCATCTCTATGTTAGCAAATAATAAAACCCTTAGTCCTAA

mRNA sequence

GAGAGAGAGAGAGAGAGAGAAACTGAAAAATGGGAGGTTGGGCCATAGCGGTTCATGGTGGGGCTGGCGTGGATCCCAATCTCCCACTTCAACGCCAAGACGATGCCAAAAAACTTCTCACTCGCTGCCTTGATCTTGGCATCCACGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTTGAACTCGTCGTCAGAGAATTGGAAACCGATCCCCTGTTCAATTCGGGGCGTGGATCTGCCCTCACAGAGGAGGGGACCGTAGAGATGGAGGCCAGCATCATGGACGGCCCAAAACGGAGATGCGGGGCGGTATCTGGACTCACCACCGTCAAAAACCCCGTCTCCCTCGCTCGTCTTGTCATGGATAAATCTCCTCATTCTTATCTGGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGGTGTGGAGATTGTGTGCAATGACTATTTCATCACCCAAGAAAACGTCGGACTGCTTGAACTAGCCAAAGAAGCCAACAGTATATGGTTGGATCATAGGGTCCCAAGCGAGACGTGTAGCGCGAGCGTGGAGACACCATTGCAAATGAACGGCTTCCCAATCAGCGTGTACGCGCCCGAGACGGTGGGGTGCGTGGTGGTTGATAGCGAGGGAAGGTGCGCCGCGGCTACATCCACTGGTGGATTGATAAACAAAAAGATTGGACGCATCGGAGACTCGCCGTTGATTGGATCAGGGACGTACGCGTGTGATGTATGTGGAGTATCTTGCACGGGAGAAGGGGAGGCGATCATAAGAGGGACTTTGGCCCGAGAAGTGGCGGCGGTGATGGAATACAAAGGGTTGGGGCTTCAAGAGGCGGTGAATTACGTGATAGAGGAAAGATTGGACGAAGGCAAGGCGGGATTGATTGCAGTGTCTAGCAATGGGGAAGTGGCGTGTGGGTTCAACACAACCGGGATGTTCAGGGGATGCGCCACGGAGGATGGGTTTATGGAGGTTGGGATTTGGTAATCTCAATAGTTACATAAATTAAGAGTGGAATCAAGTTTTATTTGGGTTTTAATGTTGTTTTAGTATGCTGTGTTCTCTTCTTGGTGAATCTTTTTTCTTCATTTTCTGAATTCAGATGCCATCTCTATGTTAGCAAATAATAAAACCCTTAGTCCTAA

Coding sequence (CDS)

ATGGGAGGTTGGGCCATAGCGGTTCATGGTGGGGCTGGCGTGGATCCCAATCTCCCACTTCAACGCCAAGACGATGCCAAAAAACTTCTCACTCGCTGCCTTGATCTTGGCATCCACGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTTGAACTCGTCGTCAGAGAATTGGAAACCGATCCCCTGTTCAATTCGGGGCGTGGATCTGCCCTCACAGAGGAGGGGACCGTAGAGATGGAGGCCAGCATCATGGACGGCCCAAAACGGAGATGCGGGGCGGTATCTGGACTCACCACCGTCAAAAACCCCGTCTCCCTCGCTCGTCTTGTCATGGATAAATCTCCTCATTCTTATCTGGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGGTGTGGAGATTGTGTGCAATGACTATTTCATCACCCAAGAAAACGTCGGACTGCTTGAACTAGCCAAAGAAGCCAACAGTATATGGTTGGATCATAGGGTCCCAAGCGAGACGTGTAGCGCGAGCGTGGAGACACCATTGCAAATGAACGGCTTCCCAATCAGCGTGTACGCGCCCGAGACGGTGGGGTGCGTGGTGGTTGATAGCGAGGGAAGGTGCGCCGCGGCTACATCCACTGGTGGATTGATAAACAAAAAGATTGGACGCATCGGAGACTCGCCGTTGATTGGATCAGGGACGTACGCGTGTGATGTATGTGGAGTATCTTGCACGGGAGAAGGGGAGGCGATCATAAGAGGGACTTTGGCCCGAGAAGTGGCGGCGGTGATGGAATACAAAGGGTTGGGGCTTCAAGAGGCGGTGAATTACGTGATAGAGGAAAGATTGGACGAAGGCAAGGCGGGATTGATTGCAGTGTCTAGCAATGGGGAAGTGGCGTGTGGGTTCAACACAACCGGGATGTTCAGGGGATGCGCCACGGAGGATGGGTTTATGGAGGTTGGGATTTGGTAA

Protein sequence

MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELETDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYLAFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEDGFMEVGIW
Homology
BLAST of ClCG05G020240 vs. NCBI nr
Match: XP_038896168.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida])

HSP 1 Score: 626.7 bits (1615), Expect = 1.1e-175
Identity = 309/322 (95.96%), Postives = 316/322 (98.14%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ DAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQHDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSG+TTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVE+V NDYFIT++NVGLLELAKEANSIWLDHRVPSETCSASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVGNDYFITEDNVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MNG  ISVYAPETVGCVVVD+EGRCAAATSTGGLINKK+GRIGDSPLIGSGTYACDVCGV
Sbjct: 181 MNGLRISVYAPETVGCVVVDTEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of ClCG05G020240 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 620.5 bits (1599), Expect = 7.9e-174
Identity = 304/322 (94.41%), Postives = 315/322 (97.83%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVE+ CNDYF+T+ENVG+LELA+EANSIWLD+RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MNG PISVYAPETVGCVVVDSEGRCAAATSTGGL+NKKIGRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of ClCG05G020240 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.8 bits (1592), Expect = 5.1e-173
Identity = 302/322 (93.79%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVE+ CNDYF+T+ENVG+LELA+EANSIWLD+RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MNG PISVYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of ClCG05G020240 vs. NCBI nr
Match: XP_023525179.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615.5 bits (1586), Expect = 2.5e-172
Identity = 303/323 (93.81%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNSSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVP-SETCSASVETPL 180
           AFSGAEKFAR+QGVE+ CNDYF+T+ENVG+LELA+EANSIWLD+RVP +E CSASVETPL
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPTNEKCSASVETPL 180

Query: 181 QMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCG 240
           QMNG PISVYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIGSGTYAC VCG
Sbjct: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV C
Sbjct: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
           GFNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 GFNTTGMFRGCATEDGFMEVGIW 323

BLAST of ClCG05G020240 vs. NCBI nr
Match: XP_008452604.1 (PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA0064417.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa] >TYK20170.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 601.7 bits (1550), Expect = 3.8e-168
Identity = 298/322 (92.55%), Postives = 305/322 (94.72%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVE+V NDYFIT+ NV LL+LAKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MN  PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of ClCG05G020240 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 532.3 bits (1370), Expect = 3.7e-150
Identity = 259/324 (79.94%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP +RQ++AK+LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETC--SASVETP 180
           AFSGAE FAR+QGVEIV N+YF+T +NVG+L+LAKEANSI  D+R+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVC 240
           +QMNG PIS+YAPETVGCVVVD +G CAA TSTGGL+NK +GRIGDSPLIG+GTYA + C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIW 323
           CGFN+ GMFRGCATEDGFMEV IW
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIW 324

BLAST of ClCG05G020240 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 369.4 bits (947), Expect = 4.2e-101
Identity = 191/323 (59.13%), Postives = 233/323 (72.14%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NP+SLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPL 180
           LAF  AE FAR  GVE V + +FIT EN+  L+ AKE N + LD+ VPS       + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPD 180

Query: 181 QMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

BLAST of ClCG05G020240 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.0e-100
Identity = 193/328 (58.84%), Postives = 239/328 (72.87%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R+   ++ L  CL +G+ AL+S    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NP+SLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHR-----VPSETCSAS 180
           LAF GA+ FA+QQGVE V + +FIT ENV  L+LA EAN + +D+       P++   A 
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQD-DAE 180

Query: 181 VETPLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYA 240
            E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA
Sbjct: 181 KELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYA 240

Query: 241 CDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSN 300
            ++C VS TG+GEAII  T+AR+VAA+ME+KGL L+EA +YV+ ER  +G  GLIAVS+ 
Sbjct: 241 NELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAA 300

Query: 301 GEVACGFNTTGMFRGCATEDGFMEVGIW 323
           GE+A  FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 GEIAMPFNTTGMFRASATEDGYSEIAIW 322

BLAST of ClCG05G020240 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.3e-99
Identity = 192/327 (58.72%), Postives = 237/327 (72.48%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R+   ++ L  CL +G+ AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NP+SLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLD---HRVPSET-CSASV 180
           LAF GA+ FA+QQGVE V + + IT ENV  L+LA EAN + +D   +  P      A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 DVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
           ++C VS TG+GE IIR T+AR+VAA+ME+KGL L+EA ++VI ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

BLAST of ClCG05G020240 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.7e-99
Identity = 190/327 (58.10%), Postives = 237/327 (72.48%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R+   ++ L  CL +GI AL++ +  +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NP+SLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSET----CSASV 180
           LAF GA+   +QQGVE V + +FIT+ENV  L+LA EAN + +D+   + T      A  
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 DVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
           ++C VS TG+GEAIIR T+AR+VAA+ME+KGL L+EA + V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

BLAST of ClCG05G020240 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.8e-174
Identity = 304/322 (94.41%), Postives = 315/322 (97.83%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVE+ CNDYF+T+ENVG+LELA+EANSIWLD+RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MNG PISVYAPETVGCVVVDSEGRCAAATSTGGL+NKKIGRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of ClCG05G020240 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.5e-173
Identity = 302/322 (93.79%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVE+ CNDYF+T+ENVG+LELA+EANSIWLD+RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MNG PISVYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of ClCG05G020240 vs. ExPASy TrEMBL
Match: A0A5D3D9K2 (Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003020 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-168
Identity = 298/322 (92.55%), Postives = 305/322 (94.72%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVE+V NDYFIT+ NV LL+LAKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MN  PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of ClCG05G020240 vs. ExPASy TrEMBL
Match: A0A1S3BV13 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC103493576 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-168
Identity = 298/322 (92.55%), Postives = 305/322 (94.72%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVE+V NDYFIT+ NV LL+LAKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGV 240
           MN  PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of ClCG05G020240 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.8e-163
Identity = 287/325 (88.31%), Postives = 310/325 (95.38%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           M GWAIAVHGGAGVDPNLPL RQ++AKKLL RCL+LGI ALRS+H AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPS---ETCSASVET 180
           AFSGAE+FARQQGVE+VCNDYFIT+ENVG+L+LAKEANSI  D+R+P+   E CSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDV 240
           PLQMNG PISVYAPETVGCVVVDS+GRCAAATSTGGL+NK+ GRIGDSPLIG+GTYAC +
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEDGFMEVGIW 323
           ACGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

BLAST of ClCG05G020240 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 532.3 bits (1370), Expect = 2.6e-151
Identity = 259/324 (79.94%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP +RQ++AK+LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETC--SASVETP 180
           AFSGAE FAR+QGVEIV N+YF+T +NVG+L+LAKEANSI  D+R+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVC 240
           +QMNG PIS+YAPETVGCVVVD +G CAA TSTGGL+NK +GRIGDSPLIG+GTYA + C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIW 323
           CGFN+ GMFRGCATEDGFMEV IW
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIW 324

BLAST of ClCG05G020240 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 369.4 bits (947), Expect = 3.0e-102
Identity = 191/323 (59.13%), Postives = 233/323 (72.14%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NP+SLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFARQQGVEIVCNDYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPL 180
           LAF  AE FAR  GVE V + +FIT EN+  L+ AKE N + LD+ VPS       + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPD 180

Query: 181 QMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

BLAST of ClCG05G020240 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 3.6e-76
Identity = 144/243 (59.26%), Postives = 176/243 (72.43%), Query Frame = 0

Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYLAFSGAEKFARQQGVEIVCN 139
           MEASIMDG  +RCGAVSGLTTV NP+SLARLVM+K+PH YLAF  AE FAR  GVE V +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFITQENVGLLELAKEANSIWLDHRVPSETCSASVETPLQMNGFPISVYAPETVGCVVV 199
            +FIT EN+  L+ AKE N + LD+ VPS       + P       I      TVGCV V
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPDNCGDSQIG-----TVGCVAV 120

Query: 200 DSEGRCAAATSTGGLINKKIGRIGDSPLIGSGTYACDVCGVSCTGEGEAIIRGTLAREVA 259
           DS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C +S TG+GE IIRGT+AR+VA
Sbjct: 121 DSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVA 180

Query: 260 AVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEDGFMEV 319
           A+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV   FNTTGMFR CA+EDG+ E+
Sbjct: 181 ALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEI 232

Query: 320 GIW 323
            IW
Sbjct: 241 AIW 232

BLAST of ClCG05G020240 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-21
Identity = 90/336 (26.79%), Postives = 143/336 (42.56%), Query Frame = 0

Query: 6   IAVHGGAGVDPNLPLQRQDDAKKLLTR-CLDLGIHALRSNHSAIDVVELVVRELETDPLF 65
           +AVH GAG         +   + ++ R CL       + +   ID V   ++ LE DP  
Sbjct: 15  VAVHVGAGYH---AAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 66  NSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARL----------VMDK 125
           N+GRGS LTE+G VE +AS+MDG     G V  +  V+N + +A L          ++ +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 126 SPHSYLAFSGAEKFARQQGVEI-----VCNDYFITQENVGLLELAKE------ANSIWLD 185
            P   L   GA ++ + + V I       + + +T+         K       A SI   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 186 HRVP-----SETCSASVETPLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKK 245
              P     +ETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K 
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKI 254

Query: 246 IGRIGDSPLIGSGTYACD--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQ 298
            GR+G +   GSG +A          + G   +G GE ++RG  ARE    +     G  
Sbjct: 255 SGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPA 314

BLAST of ClCG05G020240 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 9.1e-19
Identity = 90/328 (27.44%), Postives = 142/328 (43.29%), Query Frame = 0

Query: 6   IAVHGGAGVDPNLPLQRQDDAKKLLTR-CLDLGIHALRSNHSAIDVVELVVRELETDPLF 65
           +AVH GAG         +   + ++ R CL       + +   ID V   ++ LE DP  
Sbjct: 15  VAVHVGAGYH---AAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 66  NSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVS--LARLVMDKSPHSYLAF 125
           N+GRGS LTE+G VE +AS+MDG     G   G+  V + VS  L++L++       L  
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWLLVG 134

Query: 126 SGAEKFARQQGVEI-----VCNDYFITQENVGLLELAKE------ANSIWLDHRVP---- 185
            GA ++ + + V I       + + +T+         K       A SI      P    
Sbjct: 135 EGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTE 194

Query: 186 -SETCSASVETPLQMNGFPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSP 245
            +ETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K  GR+G + 
Sbjct: 195 NNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAA 254

Query: 246 LIGSGTYACD--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIE 298
             GSG +A          + G   +G GE ++RG  ARE    +     G   A   V+ 
Sbjct: 255 TYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLR 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896168.11.1e-17595.96probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida][more]
XP_022982271.17.9e-17494.41probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima][more]
XP_022941449.15.1e-17393.79probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447... [more]
XP_023525179.12.5e-17293.81probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo][more]
XP_008452604.13.8e-16892.55PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
Q8GXG13.7e-15079.94Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ... [more]
P502874.2e-10159.13Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
P303646.0e-10058.84Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
Q9ZSD62.3e-9958.72Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
P502888.7e-9958.10Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IYV73.8e-17494.41probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FND22.5e-17393.79probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5D3D9K21.8e-16892.55Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BV131.8e-16892.55probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1CHT11.8e-16388.31probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT3G16150.12.6e-15179.94N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.13.0e-10259.13N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.23.6e-7659.26N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.11.1e-2126.79N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.29.1e-1927.44N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 4..322
e-value: 3.1E-102
score: 341.7
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..322
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 193..322
e-value: 2.7E-43
score: 149.2
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 3..317
e-value: 6.9E-92
score: 305.8
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 3..317
e-value: 6.9E-92
score: 305.8
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 3..317
e-value: 6.9E-92
score: 305.8
NoneNo IPR availablePANTHERPTHR10188:SF36PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATEDcoord: 1..322
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 5..315
e-value: 4.87269E-122
score: 349.067
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 4..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG05G020240.1ClCG05G020240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity