ClCG05G015400 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG05G015400
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Methyltransferase
Location	CG_Chr05: 27082477 .. 27083559 (+)
RNA-Seq Expression	ClCG05G015400
Synteny	ClCG05G015400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTCTCCCCTTATTCTTCACCGGAACTTTCCTGGCCGCCGGCCTTTACTGGTTCTTCTTCATCTATGGTTCCGCCGAACGCCAAGGAAAACTCGCCGTCAACCTCTCCGGCGGTTCAATCTCCTCTGAGAAAATTCAGGACAAGTACAAACAGTACTGGTCCTTTTTCCGCCGCCCCGAGAAGATCGAAACCACCGAAAAAGTCCCCGATTTCGTAGACACCTTTTACAATTTAGTCACCGACATCTACGAGTGGGGCTGGGGCCAGTCCTTCCATTTTGCCCGTCCGATTCCAGGAAAGTCTTACAAAGAAGCCACGCGCCTCCACGAAGAAATGGTCGCCGATCTAGTCAAAGCCAAACCCGGAGATCGAATTCTCGACGTCGGATGCGGCGTCGGTGGGCCGATGCGGACCATCGCCGCCCACTCCAAAGCTAATGTTGTCGGAATCACAATCAACGACTACCAAGTTCAACGCGCAAGAATGCACAACAGAAAAGCCGGACTGGACTCAATCTGTGAAGTGGTATGTGGAAATTTCCTAGAAATGCCGTTTTCAGAAGAGAGCTTCGATGGCGCTTACGCCATTGAAGCGACGTGTCACGCGCCGAAGCTTGAAGATGTGTACTCGGAAATTTACAGAGTGTTGAAGCCGGGTTCGTTATTCGTGTCGTTGGAATGGGTTTCGACGGATAAATACGATACTGCAAATCCGGAGCATGTTAAGATCATTGAAGAAATTGCGAGAGGGAACGCTTTGCCAGGGGTAAAATCGTACAACGACGTAGCGAAAGCGGCTAAGAAAGTGGGATTTGAAGTTTTGAAAGAGGAGGATCTGGCCAAGCCGCCGGCGTTGCCATGGTGGGAACGATTGAAAATGGGCCGAGTTGCGTACTGGAGGAATCATGTCCTCGTCACCATTCTGGCCGCATTGAGAATCGCTCCGAAAGGGACTTTGGAAGTTCATGAAATGTTGGTGGAGGCAGCCGATTATTTGACCCGAAGCGGCGACGCTGGAATTTTCTCGCCGATGCATTTGATCGTCTGTAGAAAACCTGAATCGTCTAAATCCTCTTAA

mRNA sequence

ATGGATTTTCTCCCCTTATTCTTCACCGGAACTTTCCTGGCCGCCGGCCTTTACTGGTTCTTCTTCATCTATGGTTCCGCCGAACGCCAAGGAAAACTCGCCGTCAACCTCTCCGGCGGTTCAATCTCCTCTGAGAAAATTCAGGACAAGTACAAACAGTACTGGTCCTTTTTCCGCCGCCCCGAGAAGATCGAAACCACCGAAAAAGTCCCCGATTTCGTAGACACCTTTTACAATTTAGTCACCGACATCTACGAGTGGGGCTGGGGCCAGTCCTTCCATTTTGCCCGTCCGATTCCAGGAAAGTCTTACAAAGAAGCCACGCGCCTCCACGAAGAAATGGTCGCCGATCTAGTCAAAGCCAAACCCGGAGATCGAATTCTCGACGTCGGATGCGGCGTCGGTGGGCCGATGCGGACCATCGCCGCCCACTCCAAAGCTAATGTTGTCGGAATCACAATCAACGACTACCAAGTTCAACGCGCAAGAATGCACAACAGAAAAGCCGGACTGGACTCAATCTGTGAAGTGGTATGTGGAAATTTCCTAGAAATGCCGTTTTCAGAAGAGAGCTTCGATGGCGCTTACGCCATTGAAGCGACGTGTCACGCGCCGAAGCTTGAAGATGTGTACTCGGAAATTTACAGAGTGTTGAAGCCGGGTTCGTTATTCGTGTCGTTGGAATGGGTTTCGACGGATAAATACGATACTGCAAATCCGGAGCATGTTAAGATCATTGAAGAAATTGCGAGAGGGAACGCTTTGCCAGGGGTAAAATCGTACAACGACGTAGCGAAAGCGGCTAAGAAAGTGGGATTTGAAGTTTTGAAAGAGGAGGATCTGGCCAAGCCGCCGGCGTTGCCATGGTGGGAACGATTGAAAATGGGCCGAGTTGCGTACTGGAGGAATCATGTCCTCGTCACCATTCTGGCCGCATTGAGAATCGCTCCGAAAGGGACTTTGGAAGTTCATGAAATGTTGGTGGAGGCAGCCGATTATTTGACCCGAAGCGGCGACGCTGGAATTTTCTCGCCGATGCATTTGATCGTCTGTAGAAAACCTGAATCGTCTAAATCCTCTTAA

Coding sequence (CDS)

ATGGATTTTCTCCCCTTATTCTTCACCGGAACTTTCCTGGCCGCCGGCCTTTACTGGTTCTTCTTCATCTATGGTTCCGCCGAACGCCAAGGAAAACTCGCCGTCAACCTCTCCGGCGGTTCAATCTCCTCTGAGAAAATTCAGGACAAGTACAAACAGTACTGGTCCTTTTTCCGCCGCCCCGAGAAGATCGAAACCACCGAAAAAGTCCCCGATTTCGTAGACACCTTTTACAATTTAGTCACCGACATCTACGAGTGGGGCTGGGGCCAGTCCTTCCATTTTGCCCGTCCGATTCCAGGAAAGTCTTACAAAGAAGCCACGCGCCTCCACGAAGAAATGGTCGCCGATCTAGTCAAAGCCAAACCCGGAGATCGAATTCTCGACGTCGGATGCGGCGTCGGTGGGCCGATGCGGACCATCGCCGCCCACTCCAAAGCTAATGTTGTCGGAATCACAATCAACGACTACCAAGTTCAACGCGCAAGAATGCACAACAGAAAAGCCGGACTGGACTCAATCTGTGAAGTGGTATGTGGAAATTTCCTAGAAATGCCGTTTTCAGAAGAGAGCTTCGATGGCGCTTACGCCATTGAAGCGACGTGTCACGCGCCGAAGCTTGAAGATGTGTACTCGGAAATTTACAGAGTGTTGAAGCCGGGTTCGTTATTCGTGTCGTTGGAATGGGTTTCGACGGATAAATACGATACTGCAAATCCGGAGCATGTTAAGATCATTGAAGAAATTGCGAGAGGGAACGCTTTGCCAGGGGTAAAATCGTACAACGACGTAGCGAAAGCGGCTAAGAAAGTGGGATTTGAAGTTTTGAAAGAGGAGGATCTGGCCAAGCCGCCGGCGTTGCCATGGTGGGAACGATTGAAAATGGGCCGAGTTGCGTACTGGAGGAATCATGTCCTCGTCACCATTCTGGCCGCATTGAGAATCGCTCCGAAAGGGACTTTGGAAGTTCATGAAATGTTGGTGGAGGCAGCCGATTATTTGACCCGAAGCGGCGACGCTGGAATTTTCTCGCCGATGCATTTGATCGTCTGTAGAAAACCTGAATCGTCTAAATCCTCTTAA

Protein sequence

MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRRPEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCGNFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAYWRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKSS

Homology

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BLAST of ClCG05G015400 vs. NCBI nr
Match: XP_008466453.1 (PREDICTED: 24-methylenesterol C-methyltransferase 2 [Cucumis melo] >KAA0063316.1 24-methylenesterol C-methyltransferase 2 [Cucumis melo var. makuwa] >TYK31459.1 24-methylenesterol C-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 710.3 bits (1832), Expect = 8.5e-201
Identity = 341/359 (94.99%), Postives = 354/359 (98.61%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD LPLF TGT LAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDSLPLFLTGTLLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPD+VDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDYVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQVQRAR+HNRKAGLDS+CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFS+E+F+GAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDT+NP
Sbjct: 181 NFLEMPFSDETFNGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTSNP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVKSY+DVAK+AKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY
Sbjct: 241 EHVKIIEEIARGNALPGVKSYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVTILA LRIAPKGTL+VHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPES K+
Sbjct: 301 WRNHILVTILATLRIAPKGTLDVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESPKT 359

[Close]

BLAST of ClCG05G015400 vs. NCBI nr
Match: XP_038899067.1 (24-methylenesterol C-methyltransferase 2 [Benincasa hispida])

HSP 1 Score: 708.0 bits (1826), Expect = 4.2e-200
Identity = 342/359 (95.26%), Postives = 352/359 (98.05%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD LPLF TGT LAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDSLPLFLTGTLLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLV 
Sbjct: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVN 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AK GDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQVQRAR+HN+KAGLDS+CEVVCG
Sbjct: 121 AKSGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVQRARLHNKKAGLDSLCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFS+ESF+GAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDT NP
Sbjct: 181 NFLEMPFSDESFNGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTTNP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVKSY+DVAK+AKKVGFEVLKEEDLAKPPALPWW RLKMGRVAY
Sbjct: 241 EHVKIIEEIARGNALPGVKSYDDVAKSAKKVGFEVLKEEDLAKPPALPWWGRLKMGRVAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSK+
Sbjct: 301 WRNHILVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKT 359

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BLAST of ClCG05G015400 vs. NCBI nr
Match: XP_004136483.1 (24-methylenesterol C-methyltransferase 2 [Cucumis sativus] >KGN60066.1 hypothetical protein Csa_002528 [Cucumis sativus])

HSP 1 Score: 704.1 bits (1816), Expect = 6.1e-199
Identity = 338/359 (94.15%), Postives = 352/359 (98.05%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD LP F TGT LAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDSLPFFLTGTLLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPD+VDTFYNLVTDIYEWGWGQSFHFARPIPGK+YKEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDYVDTFYNLVTDIYEWGWGQSFHFARPIPGKTYKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAA+SKANVVGITINDYQVQRAR+HNRKAGLDS+CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAYSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFS+E+F+GAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP
Sbjct: 181 NFLEMPFSDETFNGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVK Y+DVAK+AKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY
Sbjct: 241 EHVKIIEEIARGNALPGVKLYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVTILA LRIAPKGTL+VHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPES K+
Sbjct: 301 WRNHILVTILATLRIAPKGTLDVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESPKT 359

[Close]

BLAST of ClCG05G015400 vs. NCBI nr
Match: XP_022976336.1 (24-methylenesterol C-methyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 701.0 bits (1808), Expect = 5.1e-198
Identity = 338/359 (94.15%), Postives = 351/359 (97.77%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MDFLPLF TGT +AA LYWF FIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDFLPLFITGTLVAATLYWFLFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKS KEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSDKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQV RAR+HN+KAGLD++CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFSE +FDGAYAIEATCHAPKLEDVY+EIYRVLKPGSLFVSLEWVSTDKYDTANP
Sbjct: 181 NFLEMPFSEGTFDGAYAIEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHV+IIEEIARGNALPGVKSY+DVAKAAKKVGFEVLKEEDLAKPPALPWW+RLKMGR+AY
Sbjct: 241 EHVEIIEEIARGNALPGVKSYDDVAKAAKKVGFEVLKEEDLAKPPALPWWDRLKMGRLAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNHVLVT+LAA RIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS
Sbjct: 301 WRNHVLVTVLAAFRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 359

[Close]

BLAST of ClCG05G015400 vs. NCBI nr
Match: KAG6591249.1 (24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697.2 bits (1798), Expect = 7.4e-197
Identity = 336/359 (93.59%), Postives = 349/359 (97.21%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MDFLPLF TGT +AA LYWF FIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDFLPLFITGTLVAATLYWFLFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIET EKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKS KEATRLHEEMVADLVK
Sbjct: 61  PEKIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSDKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQV RAR+HN+KAGLD++CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFSE +FDGAYAIEATCHAPKLEDVY+EIYRVLKPGSLFVSLEWVSTDKYDTANP
Sbjct: 181 NFLEMPFSEGTFDGAYAIEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVKSY+DVAKAAKKVGF+VLKEEDLAKPPALPWW RLKMGR+AY
Sbjct: 241 EHVKIIEEIARGNALPGVKSYHDVAKAAKKVGFQVLKEEDLAKPPALPWWGRLKMGRLAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVT+LAA RIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS
Sbjct: 301 WRNHILVTLLAAFRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 359

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BLAST of ClCG05G015400 vs. ExPASy Swiss-Prot
Match: Q39227 (24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=SMT2 PE=1 SV=2)

HSP 1 Score: 578.9 bits (1491), Expect = 3.9e-164
Identity = 260/360 (72.22%), Postives = 318/360 (88.33%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD L LFFTG  +A G+YWF  + G AER+GK AV+LSGGSIS+EK+QD YKQYWSFFRR
Sbjct: 1   MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           P++IET EKVPDFVDTFYNLVTDIYEWGWGQSFHF+  IPGKS+K+ATRLHEEM  DL++
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
            KPG +ILDVGCGVGGPMR IA+HS+ANVVGITIN+YQV RAR+HN+KAGLD++CEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFL+MPF + SFDGAY+IEATCHAPKLE+VY+EIYRVLKPGS++VS EWV+T+K+   + 
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHV++I+ I RG+ALPG+++Y D+A+ AKKVGFE++KE+DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKSS 360
           WRNH++V IL+A+ +APKGT++VHEML + ADYLTR G+ GIFSPMH+I+CRKPES + S
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPESPEES 360

[Close]

BLAST of ClCG05G015400 vs. ExPASy Swiss-Prot
Match: Q94JS4 (24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=SMT3 PE=2 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 3.0e-156
Identity = 249/357 (69.75%), Postives = 305/357 (85.43%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD + L+ T   +A  +YWF  + G AER+GK A +LSGGSIS+EK++D Y QYWSFFR+
Sbjct: 1   MDSVALYCTAGLIAGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFRK 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           P++IE+ EKVPDFVDTFYNLVTDIYEWGWGQSFHF+  +PGKS K+ATR+HEEM  DL+K
Sbjct: 61  PKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
            KPG +ILD GCGVGGPMR IAAHSKA V GITIN+YQVQRA++HN+KAGLDS+C VVCG
Sbjct: 121 VKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFL+MPF E +FDGAY+IEATCHAPKLE+VYSEI+RV+KPGSLFVS EWV+T+KY   + 
Sbjct: 181 NFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDE 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EH  +I+ I RG+ALPG++SY D+A  AKKVGFEV+KE+DLAKPP+ PWW RLKMGR+AY
Sbjct: 241 EHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESS 358
           WRNHV+V IL+A+ +APKGT++VH+ML + ADYLTR G+ GIFSPMH+I+CRKPE +
Sbjct: 301 WRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGETGIFSPMHMILCRKPEKA 357

[Close]

BLAST of ClCG05G015400 vs. ExPASy Swiss-Prot
Match: O82427 (24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Smt2-1 PE=2 SV=2)

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-144
Identity = 238/346 (68.79%), Postives = 288/346 (83.24%), Query Frame = 0


Query: 17  LYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRRPEKIETT----EKVPD 76
           +YWF ++ G+AE +GK AV+L  GSI+++K++DKY QYWSFFRRP++  TT    EKVP 
Sbjct: 18  VYWFVWVMGAAEVKGKRAVDLKMGSITNDKVKDKYTQYWSFFRRPKETATTEASAEKVPA 77

Query: 77  FVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGC 136
           FVDTFYNLVTDIYEWGWGQSFHF+  +PG+S++EATR+HEE VADL++AKPG R+LDVGC
Sbjct: 78  FVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHREATRVHEERVADLLQAKPGHRLLDVGC 137

Query: 137 GVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCGNFLEMPFSEESF 196
           GVGGPMR IAAHS +NVVGITIN+YQV RAR HNRKAGLDS CEVVCGNFL MPFS+ SF
Sbjct: 138 GVGGPMRAIAAHSGSNVVGITINEYQVNRARAHNRKAGLDSRCEVVCGNFLSMPFSDASF 197

Query: 197 DGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARG 256
           DGAY+IEATCHAP+L+DVY E++RVLKPG L+VS EWV+T  Y   NPEHV+ I  I RG
Sbjct: 198 DGAYSIEATCHAPRLQDVYGEVFRVLKPGGLYVSYEWVTTSLYRADNPEHVEAIHGIERG 257

Query: 257 NALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAYWRNHVLVTILAA 316
           +ALPG++  +++A  AK+VGFEVLKE DLA PPALPWW RLKMGR+AYWRN ++V +L  
Sbjct: 258 DALPGLRRQDEIASIAKEVGFEVLKELDLALPPALPWWTRLKMGRIAYWRNSLVVRVLTM 317

Query: 317 LRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSK 359
           LRIAPKG  EVHEML E A +LTR G+ GIF+PMH+++ RKP  SK
Sbjct: 318 LRIAPKGVCEVHEMLYETAQHLTRGGETGIFTPMHMVLLRKPVESK 363

[Close]

BLAST of ClCG05G015400 vs. ExPASy Swiss-Prot
Match: H2E7T7 (Botryococcene C-methyltransferase OS=Botryococcus braunii OX=38881 GN=TMT-3 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 9.4e-78
Identity = 142/324 (43.83%), Postives = 203/324 (62.65%), Query Frame = 0


Query: 40  GSISSEKIQDKYKQYWSFFRRPEK----IETTEKVPDFVDTFYNLVTDIYEWGWGQSFHF 99
           G ++ EK+ +    Y   + + EK    ++   K+    +TFY+L+TD YEWGWG  FHF
Sbjct: 56  GGVAFEKVAEVVADYSDSYGQTEKGELIVKDNNKIVSLANTFYDLITDGYEWGWGSGFHF 115

Query: 100 ARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRTIAAHSKANVVGITIN 159
           +  +PG S+  +  LHE  +A  ++ KPG ++LDVGCGVG P RT+AA S A V GITIN
Sbjct: 116 SHRLPGMSFNASQLLHESRMASFLRLKPGMQVLDVGCGVGNPGRTVAACSGAVVTGITIN 175

Query: 160 DYQVQRARMHNRKAGLDSICEVVCGNFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIY 219
            YQ++RA +H ++AGL    + V GNF  MPF ++SFD A+A+++TCHAPKLEDVYSE++
Sbjct: 176 AYQIKRAELHTKRAGLVGYFKPVQGNFCAMPFQDKSFDAAFAMDSTCHAPKLEDVYSEVF 235

Query: 220 RVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEV 279
           RVLKPG+ F + EWVST  YD+ NPEHVK +  I  GN LP ++S+    +A K VGF +
Sbjct: 236 RVLKPGAYFATYEWVSTKNYDSNNPEHVKCMNSIILGNGLPNIRSWKQAEEAGKNVGFNL 295

Query: 280 LKEEDLA--KPPALPWWE-RLKMGRVAYWRNH-VLVTILAALRIAPKGTLEVHEMLVEAA 339
           L   D+A   P   PW+    +M     +R H   +   + L + P  + +   +L E A
Sbjct: 296 LTSLDMATNSPIGKPWYSVPERMVNWGLFRFHKACIRTASTLHLLPPESWKFFYILAEMA 355

Query: 340 DYLTRSGDAGIFSPMHLIVCRKPE 356
           + L + G   IF+PMHL++ +KPE
Sbjct: 356 ENLVKGGQWDIFTPMHLLIFQKPE 379

[Close]

BLAST of ClCG05G015400 vs. ExPASy Swiss-Prot
Match: H2E7T6 (Squalene methyltransferase 2 OS=Botryococcus braunii OX=38881 GN=TMT-2 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.3e-74
Identity = 143/331 (43.20%), Postives = 198/331 (59.82%), Query Frame = 0


Query: 35  VNLSGGSISSEKIQDKYKQYWSFFRRPE----KIETTEKVPDFVDTFYNLVTDIYEWGWG 94
           ++L+ G I  EK+ + +  Y   + +       ++ T KV    +TFY+ VTD YEW WG
Sbjct: 50  LDLTAGGIPFEKVGEVFNDYDKSYGKGTHGELHVQDTNKVFQLANTFYDFVTDGYEWAWG 109

Query: 95  QSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRTIAAHSKANVV 154
            SFHF++ +PG S+  +  LHE  +A  ++ KPG   LDVGCGVG P RT+AA S A V 
Sbjct: 110 SSFHFSQRMPGLSHAASQMLHESRMASYLRLKPGMTCLDVGCGVGNPGRTVAACSGAVVT 169

Query: 155 GITINDYQVQRARMHNRKAGLDSICEVVCGNFLEMPFSEESFDGAYAIEATCHAPKLEDV 214
           GITIN YQ+QRA  HNR+ GL    +   GNF  MPF  +SFD A+A++ATCHAPKLEDV
Sbjct: 170 GITINKYQIQRAEYHNRRTGLVGFFKPTVGNFCNMPFDAKSFDAAFAMDATCHAPKLEDV 229

Query: 215 YSEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPGVKSYNDVAKAAKK 274
           Y E++RVLKPG  F + EWVST  YD  N  HVK++  I  GN LP ++S+    +A + 
Sbjct: 230 YGEVFRVLKPGGFFATYEWVSTKNYDPTNTRHVKVMNSIIFGNGLPNIRSWKQAEEAGEN 289

Query: 275 VGFEVLKEEDLAKPPAL--PWWE----RLKMGRVAYWRNHVLVTILAALRIAPKGTLEVH 334
           VGF++L   DLA  P +  PW+      +K G +   +   LV    +L + P  + +V 
Sbjct: 290 VGFKLLTSFDLATAPPVGKPWYYVPELMVKYGLLKIQK--ALVRGACSLGLLPDQSWKVC 349

Query: 335 EMLVEAADYLTRSGDAGIFSPMHLIVCRKPE 356
            M+ +    L   G   IF+PMHL++ +KPE
Sbjct: 350 NMVADMVPNLVEGGATDIFTPMHLLIFQKPE 378

[Close]

BLAST of ClCG05G015400 vs. ExPASy TrEMBL
Match: A0A5A7VA53 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007740 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.1e-201
Identity = 341/359 (94.99%), Postives = 354/359 (98.61%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD LPLF TGT LAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDSLPLFLTGTLLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPD+VDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDYVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQVQRAR+HNRKAGLDS+CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFS+E+F+GAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDT+NP
Sbjct: 181 NFLEMPFSDETFNGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTSNP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVKSY+DVAK+AKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY
Sbjct: 241 EHVKIIEEIARGNALPGVKSYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVTILA LRIAPKGTL+VHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPES K+
Sbjct: 301 WRNHILVTILATLRIAPKGTLDVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESPKT 359

[Close]

BLAST of ClCG05G015400 vs. ExPASy TrEMBL
Match: A0A1S3CRG4 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503852 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.1e-201
Identity = 341/359 (94.99%), Postives = 354/359 (98.61%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD LPLF TGT LAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDSLPLFLTGTLLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPD+VDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDYVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQVQRAR+HNRKAGLDS+CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFS+E+F+GAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDT+NP
Sbjct: 181 NFLEMPFSDETFNGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTSNP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVKSY+DVAK+AKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY
Sbjct: 241 EHVKIIEEIARGNALPGVKSYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVTILA LRIAPKGTL+VHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPES K+
Sbjct: 301 WRNHILVTILATLRIAPKGTLDVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESPKT 359

[Close]

BLAST of ClCG05G015400 vs. ExPASy TrEMBL
Match: A0A0A0LJ82 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G875420 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 2.9e-199
Identity = 338/359 (94.15%), Postives = 352/359 (98.05%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD LP F TGT LAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDSLPFFLTGTLLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPD+VDTFYNLVTDIYEWGWGQSFHFARPIPGK+YKEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDYVDTFYNLVTDIYEWGWGQSFHFARPIPGKTYKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAA+SKANVVGITINDYQVQRAR+HNRKAGLDS+CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAYSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFS+E+F+GAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP
Sbjct: 181 NFLEMPFSDETFNGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVK Y+DVAK+AKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY
Sbjct: 241 EHVKIIEEIARGNALPGVKLYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVTILA LRIAPKGTL+VHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPES K+
Sbjct: 301 WRNHILVTILATLRIAPKGTLDVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESPKT 359

[Close]

BLAST of ClCG05G015400 vs. ExPASy TrEMBL
Match: A0A6J1IGM5 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476770 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.5e-198
Identity = 338/359 (94.15%), Postives = 351/359 (97.77%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MDFLPLF TGT +AA LYWF FIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MDFLPLFITGTLVAATLYWFLFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKS KEATRLHEEMVADLVK
Sbjct: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSDKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQV RAR+HN+KAGLD++CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFSE +FDGAYAIEATCHAPKLEDVY+EIYRVLKPGSLFVSLEWVSTDKYDTANP
Sbjct: 181 NFLEMPFSEGTFDGAYAIEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHV+IIEEIARGNALPGVKSY+DVAKAAKKVGFEVLKEEDLAKPPALPWW+RLKMGR+AY
Sbjct: 241 EHVEIIEEIARGNALPGVKSYDDVAKAAKKVGFEVLKEEDLAKPPALPWWDRLKMGRLAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNHVLVT+LAA RIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS
Sbjct: 301 WRNHVLVTVLAAFRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 359

[Close]

BLAST of ClCG05G015400 vs. ExPASy TrEMBL
Match: A0A6J1FB58 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443718 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.0e-197
Identity = 335/359 (93.31%), Postives = 349/359 (97.21%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           M+FLPLF TGT +AA LYWF FIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR
Sbjct: 1   MEFLPLFITGTLVAATLYWFLFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           PEKIET EKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKS KEATRLHEEMVADLVK
Sbjct: 61  PEKIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSDKEATRLHEEMVADLVK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
           AKPGDRILDVGCGVGGPMR+IAAHSKANVVGITINDYQV RAR+HN+KAGLD++CEVVCG
Sbjct: 121 AKPGDRILDVGCGVGGPMRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFLEMPFSE +FDGAYAIEATCHAPKLEDVY+EIYRVLKPGSLFVSLEWVSTDKYDTANP
Sbjct: 181 NFLEMPFSEGTFDGAYAIEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANP 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHVKIIEEIARGNALPGVKSY+DVAKAAKKVGF+VLKEEDLAKPPALPWW RLKMGR+AY
Sbjct: 241 EHVKIIEEIARGNALPGVKSYDDVAKAAKKVGFQVLKEEDLAKPPALPWWGRLKMGRLAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 360
           WRNH+LVT+LAA RIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS
Sbjct: 301 WRNHILVTVLAAFRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKS 359

[Close]

BLAST of ClCG05G015400 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 578.9 bits (1491), Expect = 2.7e-165
Identity = 260/360 (72.22%), Postives = 318/360 (88.33%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD L LFFTG  +A G+YWF  + G AER+GK AV+LSGGSIS+EK+QD YKQYWSFFRR
Sbjct: 1   MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           P++IET EKVPDFVDTFYNLVTDIYEWGWGQSFHF+  IPGKS+K+ATRLHEEM  DL++
Sbjct: 61  PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
            KPG +ILDVGCGVGGPMR IA+HS+ANVVGITIN+YQV RAR+HN+KAGLD++CEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFL+MPF + SFDGAY+IEATCHAPKLE+VY+EIYRVLKPGS++VS EWV+T+K+   + 
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EHV++I+ I RG+ALPG+++Y D+A+ AKKVGFE++KE+DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESSKSS 360
           WRNH++V IL+A+ +APKGT++VHEML + ADYLTR G+ GIFSPMH+I+CRKPES + S
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPESPEES 360

[Close]

BLAST of ClCG05G015400 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 552.7 bits (1423), Expect = 2.1e-157
Identity = 249/357 (69.75%), Postives = 305/357 (85.43%), Query Frame = 0


Query: 1   MDFLPLFFTGTFLAAGLYWFFFIYGSAERQGKLAVNLSGGSISSEKIQDKYKQYWSFFRR 60
           MD + L+ T   +A  +YWF  + G AER+GK A +LSGGSIS+EK++D Y QYWSFFR+
Sbjct: 1   MDSVALYCTAGLIAGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFRK 60

Query: 61  PEKIETTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKSYKEATRLHEEMVADLVK 120
           P++IE+ EKVPDFVDTFYNLVTDIYEWGWGQSFHF+  +PGKS K+ATR+HEEM  DL+K
Sbjct: 61  PKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIK 120

Query: 121 AKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLDSICEVVCG 180
            KPG +ILD GCGVGGPMR IAAHSKA V GITIN+YQVQRA++HN+KAGLDS+C VVCG
Sbjct: 121 VKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCG 180

Query: 181 NFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANP 240
           NFL+MPF E +FDGAY+IEATCHAPKLE+VYSEI+RV+KPGSLFVS EWV+T+KY   + 
Sbjct: 181 NFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDE 240

Query: 241 EHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAY 300
           EH  +I+ I RG+ALPG++SY D+A  AKKVGFEV+KE+DLAKPP+ PWW RLKMGR+AY
Sbjct: 241 EHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAY 300

Query: 301 WRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPESS 358
           WRNHV+V IL+A+ +APKGT++VH+ML + ADYLTR G+ GIFSPMH+I+CRKPE +
Sbjct: 301 WRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGETGIFSPMHMILCRKPEKA 357

[Close]

BLAST of ClCG05G015400 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 270.0 bits (689), Expect = 2.7e-72
Identity = 141/335 (42.09%), Postives = 196/335 (58.51%), Query Frame = 0


Query: 33  LAVNLSGGSISSEKIQ--DKYKQYWSFFRRPEKIETTEKVPDFVDTFYNLVTDIYEWGWG 92
           LA NL G    S+ +   +KY+QY  F    E+ E      D V+ +Y+L T  YE+GWG
Sbjct: 3   LASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEE-ERKANYTDMVNKYYDLATSFYEYGWG 62

Query: 93  QSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRTIAAHSKANVV 152
           +SFHFA+   G+S +E+ + HE  +A  +  +PG ++LDVGCG+GGP+R IA  S + V 
Sbjct: 63  ESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVT 122

Query: 153 GITINDYQVQRARMHNRKAGLDSICEVVCGNFLEMPFSEESFDGAYAIEATCHAPKLEDV 212
           G+  N+YQ+ R +  NR AG+D  C  V  +F++MPF E SFD  YAIEATCHAP     
Sbjct: 123 GLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGC 182

Query: 213 YSEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPGVKSYNDVAKAAKK 272
           Y EIYRVLKPG  F + EW  TD +D  N EH KI  EI  G+ LP ++      +A K+
Sbjct: 183 YKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQ 242

Query: 273 VGFEVLKEEDLAKPPALPWW----------ERLKMGRVAYWRNHVLVTILAALRIAPKGT 332
            GFEV+ E+DLAK   +PW+             ++  V  +    +V IL  +R+AP+G+
Sbjct: 243 AGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQGS 302

Query: 333 LEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPE 356
             V   L +AA+ L   G   IF+PM+  + RKPE
Sbjct: 303 QRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKPE 336

[Close]

BLAST of ClCG05G015400 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 270.0 bits (689), Expect = 2.7e-72
Identity = 141/335 (42.09%), Postives = 196/335 (58.51%), Query Frame = 0


Query: 33  LAVNLSGGSISSEKIQ--DKYKQYWSFFRRPEKIETTEKVPDFVDTFYNLVTDIYEWGWG 92
           LA NL G    S+ +   +KY+QY  F    E+ E      D V+ +Y+L T  YE+GWG
Sbjct: 3   LASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEE-ERKANYTDMVNKYYDLATSFYEYGWG 62

Query: 93  QSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRTIAAHSKANVV 152
           +SFHFA+   G+S +E+ + HE  +A  +  +PG ++LDVGCG+GGP+R IA  S + V 
Sbjct: 63  ESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVT 122

Query: 153 GITINDYQVQRARMHNRKAGLDSICEVVCGNFLEMPFSEESFDGAYAIEATCHAPKLEDV 212
           G+  N+YQ+ R +  NR AG+D  C  V  +F++MPF E SFD  YAIEATCHAP     
Sbjct: 123 GLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGC 182

Query: 213 YSEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPGVKSYNDVAKAAKK 272
           Y EIYRVLKPG  F + EW  TD +D  N EH KI  EI  G+ LP ++      +A K+
Sbjct: 183 YKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQ 242

Query: 273 VGFEVLKEEDLAKPPALPWW----------ERLKMGRVAYWRNHVLVTILAALRIAPKGT 332
            GFEV+ E+DLAK   +PW+             ++  V  +    +V IL  +R+AP+G+
Sbjct: 243 AGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQGS 302

Query: 333 LEVHEMLVEAADYLTRSGDAGIFSPMHLIVCRKPE 356
             V   L +AA+ L   G   IF+PM+  + RKPE
Sbjct: 303 QRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKPE 336

[Close]

BLAST of ClCG05G015400 vs. TAIR 10
Match: AT1G64970.1 (gamma-tocopherol methyltransferase )

HSP 1 Score: 87.8 bits (216), Expect = 1.9e-17
Identity = 76/305 (24.92%), Postives = 131/305 (42.95%), Query Frame = 0


Query: 66  TTEKVPDFVDTFYNLVTDIYEWGWGQSFHFARPIPGKS-------YKEA-TRLHEEM--- 125
           +TE +   +  FYN  + ++E  WG   H     P  S       +KEA  R+ EE    
Sbjct: 56  STEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESLRF 115

Query: 126 --VADLVKAKPGDRILDVGCGVGGPMRTIAAHSKANVVGITINDYQVQRARMHNRKAGLD 185
             V D  + K   +++DVGCG+GG  R +A+   A  +GIT++  Q +RA        L 
Sbjct: 116 AGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLA 175

Query: 186 SICEVVCGNFLEMPFSEESFDGAYAIEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVST 245
                   + L+ PF +  FD  +++E+  H P       E+ RV  PG   + + W   
Sbjct: 176 HKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWCHR 235

Query: 246 D---KYDTANPEHVKIIEEIARGNALPGVKSYNDVAKAAKKVGFEVLKEEDLAKPPALPW 305
           +     +   P    I+++I +   LP   S +D     +    + +K  D ++  A P+
Sbjct: 236 NLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENVA-PF 295

Query: 306 WERLKMGRVAYWRNHVLVTILAALRIAPKGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 355
           W  + +     W+   LV++L +   + KG L +  M+      + + G         +I
Sbjct: 296 WPAV-IRTALTWKG--LVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFG---------II 347

The following BLAST results are available for this feature:

BLAST of ClCG05G015400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008466453.1	8.5e-201	94.99	PREDICTED: 24-methylenesterol C-methyltransferase 2 [Cucumis melo] >KAA0063316.1...	[more]
XP_038899067.1	4.2e-200	95.26	24-methylenesterol C-methyltransferase 2 [Benincasa hispida]	[more]
XP_004136483.1	6.1e-199	94.15	24-methylenesterol C-methyltransferase 2 [Cucumis sativus] >KGN60066.1 hypotheti...	[more]
XP_022976336.1	5.1e-198	94.15	24-methylenesterol C-methyltransferase 2 [Cucurbita maxima]	[more]
KAG6591249.1	7.4e-197	93.59	24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of ClCG05G015400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39227	3.9e-164	72.22	24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=SMT2...	[more]
Q94JS4	3.0e-156	69.75	24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=SMT3...	[more]
O82427	1.2e-144	68.79	24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica OX=3994...	[more]
H2E7T7	9.4e-78	43.83	Botryococcene C-methyltransferase OS=Botryococcus braunii OX=38881 GN=TMT-3 PE=1...	[more]
H2E7T6	1.3e-74	43.20	Squalene methyltransferase 2 OS=Botryococcus braunii OX=38881 GN=TMT-2 PE=1 SV=1	[more]

BLAST of ClCG05G015400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VA53	4.1e-201	94.99	Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S3CRG4	4.1e-201	94.99	Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503852 PE=3 SV=1	[more]
A0A0A0LJ82	2.9e-199	94.15	Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G875420 PE=3 SV=1	[more]
A0A6J1IGM5	2.5e-198	94.15	Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476770 PE=3 SV=1	[more]
A0A6J1FB58	8.0e-197	93.31	Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443718 PE=3 SV=1	[more]

BLAST of ClCG05G015400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20330.1	2.7e-165	72.22	sterol methyltransferase 2	[more]
AT1G76090.1	2.1e-157	69.75	sterol methyltransferase 3	[more]
AT5G13710.1	2.7e-72	42.09	sterol methyltransferase 1	[more]
AT5G13710.2	2.7e-72	42.09	sterol methyltransferase 1	[more]
AT1G64970.1	1.9e-17	24.92	gamma-tocopherol methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013705	Sterol methyltransferase C-terminal	PFAM	PF08498	Sterol_MT_C	coord: 292..355 e-value: 8.6E-23 score: 80.3
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 44..308 e-value: 2.9E-51 score: 176.2
None	No IPR available	PANTHER	PTHR44742:SF6	METHYLTRANSFERASE	coord: 1..356
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 126..230 e-value: 3.77699E-17 score: 74.3886
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 128..225 e-value: 3.5E-20 score: 72.5
IPR044625	Sterol methyltransferase SMT2/3-like	PANTHER	PTHR44742	FAMILY NOT NAMED	coord: 1..356
IPR030384	SAM-dependent methyltransferase SMT-type	PROSITE	PS51685	SAM_MT_ERG6_SMT	coord: 77..354 score: 106.053833
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 76..355

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG05G015400.1	ClCG05G015400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0090378	seed trichome elongation
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0006694	steroid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008168	methyltransferase activity