ClCG04G010350 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG04G010350
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionLipid droplet-associated hydrolase
LocationCG_Chr04: 25298144 .. 25302717 (-)
RNA-Seq ExpressionClCG04G010350
SyntenyClCG04G010350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCCAGCAAGTTCTGCAATCTTTTAGTAAAAGCCCTGTGGAGTTTCGTTTATGCAATGTATCAGGGTAATTGATTATTCTCGTTTTTATTCACTGAAATTTTCTCTTGGAATTGAGTTTTCTTGCATTGGATCCCCTTACCGTTTCTGGGTTTTGATAGTTACACCAATGAATTACTGGAGATTCATTCCGACGATCCATCATTGCATGTATTGTTCATACCTGGAAATCCAGGTGCATCTGCTTTTTGGGGATGTTGGTTTTCGATGCTTCTGTTGATTTTTGTATAATTTCTGAATGTAAATTGTTATGGGCTTTGTTTCCAAGACAGGGATTATTTCATTTTATAAAGATTTTGTTGAATCACTGTATCAGTTGCTTGGAGGCCGTGTGTCTATAACAGGCAAGATATGAGTTGTTGACATTATTCATATCCCTTTCATTTTTTCTTAGAAAGGATTGAATCTTTTATATTTTAGCTGACATTTTTTCTTGTTCTGTTTATCTTCTTTGGCCCTCCTTTTGTCTATTGTCAGCTATTGGACATATATGTCAAACGAAGAAGGTACTTTGTCATGGAACATACGTTTTAGCACTCATTTTCTTCTAAGTGGCAAAGTCTTTTAGAAATGATGTTTCTGTGTACTGAAAATTTTTATATTTGAAACATAAATGGCTCTGTTTAATTAGTGGTTTTGGTTAGTTATGCTTAATTTTCATGTTTGAAAAAGGAAATTAGTAGATGTTGGTCGGACGGGTATAATGAAGTTTCTTTTATAAATTTCCCCGGTGATTTTATGTAATGGATATGATGCTTCCTCTCTTTTAGGACTGGGAAGGTGGAAGGTTATTTTCGTTACAAGAACAAATTGATCATAAGGTAACTAGCTGTTTTCTCTGTCCATTTGATTGAATTGTTGATGGCTTAAGGTGGCAAGTGGTCCATGGAAAATTTAAATTTTGAAGTTCCTTTATAAGCTAGTGGACTTCATTTTTCTTGTCCAAGATAGGTATGGAATTGGAGATGGCATCGCAGAATTTTGAAATTAACATCTCTTGCTCAAGATTACTGTTCATTTCATTATTATTTGTTCCCTGCAGGTCCCTTTCTCTTTCATATTTCAATTGTTGATACCAGAATTCTGTATGGGCTGAACAATATTCATCTTTCTTTTGCAGATTGAATTTGTTAGACAGGAGTTACCAAATAAAGACATCCCGTTACTACTGGTAATGATACCTTTATGATTACAATTGATGCTATGTTTTCACACTAGTCTTAAAAGAGTTGCACAGAGAAAAATTTTAAATTGTATTATCCTGATATTTTCTGAGTAAATGTCAGGTCAGTTATCTTTTACTAGTAAGCTCCGCAACTTGTATAAAATTTGATTCTGTTTTAACCCATACATAATAGTTCCTTGTGGTGCTTGTATTCTTCTTGTTACTTTGCTAAAAACAAAACAATCCACTTGATTGTTTGTACTTGTGCATTATTGGCATCTTGAATATAATTAATTGGAAATTGATATGAGATGATACAGGCCTTGAGAATAAGAATGACGGTTAGATACTTGTTTGTTAAAACGGTTTTCTTTTTTTTTTCCCCGTTCTTGGTAACTAACTCGTCAGTTGTCTTTAAAGGTAGGGCATTCTGTTGGATCGTATATCTCCATTGAATTGTACCGTAGGTTCCAGGATCGGGTATTGCCATAAACTCTTTGTGCCTCTTATGTGTTCCCAACTTTCAAGAGATGGTTCAATAGCCATTCTTCGTGATCCTATAGGTGGTGTATTGCATTGGATTGCATCCATTTATGATGGTGAACAGACAGTCACGTCAGCAATTTTTTATTGAAAAACTTGCTGGGTATGCACGTTATTTTTTCTGATAACATGAACTATGTATGCCTTCTTGCATCTACATTTCAGATTTTTTAGCAGTGATTTTGTTGCATAACATGGTTTTCAAAATTTCCCATGTCACTTATATAGTTATATATTGGTATCAAAGGAGTGCAGTGTCATGGCATCCATTTTCATTAAATTAGAACGTGTATCCATAAAACCCTTTCCACTCATTCTGATTATAGGACCCTTCTAACAATCTTGTCCACTTCATCGAGTTCAAAGTTCCAAACTTAGCCTTATAGATGGAGCTTTTCAGTTATGTCCATTTTTTCGATACTGGCATGTTGAAATAAATACTCTATTTAGTTGGTAGCAAATGATAAATATAAATGAAATGACAGTTGATAGGATTCGAGTTTGAGACTTTTGACCCTCAAAAGTCATACTGCCTCAAACAGTTACACACTGCATCATGTGATGTGGCTTCGTTGGGCTGTGGGCTTTATCAAATATCTAGAATCCCCTCAGAACCACAAACTGGTGTAATTTGGCAATTTTCGGATTTCTGTTGGTTGTGTGGGGAGCATTTGCATTGTCCAAGTGGGTATGCACTTGGGTTGAACCAATTTCGAGTCACTTAGATTAATGGATGTGTGCTGAGTCTATATCCAGTTAAGTGTGTCATATTATTTTGTAGGACTCGGCAAGTTTCATGGCTTTGTGACATTGTTAACTATTTAAACAGCCTTTTGGCTGCTTTGTAATTGACCTTAATAATTAATAAAATTGGACCATGTAGAAGTCCTTCGTTTCCATACATTGTCCACAACTGGACCTTAATCCTTTAGGCATCTACTACTATGGCTGATCTTATTTTGGTCCATGCGTGATTACAAACTAACATATTAGGTCATTTTTAAGTTTTGGTATTGCAAGTAGTATCTTTCTGAGCACCACCCAGTTCATTCGGATGATCTGTTATGTTGTTTTCTTCCACAAAATATACTTTTGAGCACATGAAAATTTTGTATTGCAGATCCCCACTGCTATCAACTTTGTTTTCTTCTTTTGTTGCGCTGTTGGGTGTATTACCAATTCGAGCTTCAAGTTTTGTGGTGAGAAAAACTATTGGGAAGTCATGGTCCAGAACTGCTTGTGAGGCTGCTTGCTCTCATTTGCTAAAGGTGAAATGAAATCTGATTATTTTGTGTAGCATGTGCTATTTTAGTATCCACTCGTAACTGCTACAAATTTTGGTTTGACGACATGACGGTTTGTTCTAATATTTGGAATATTATCTTTGTTTTCTACAGTACCATAGCATGCGAAATGTGCTATATTTCGCAATGACGGAGTTTGAGAAGGTATTCACTGTAAACAATCACTTTAGTTTGGTGAACATGTTTAGGGCGTGTTGGAAACCATGACACTCCTGACACTTAGTTGCTCATGTATGAAGAAATTTGCTTCATTTGTGTCATTTTTTTTTAATTTGAGAACCATTGAACAAAAAGAACCATAAGAGAATTAATAAAACTTGAGAGTGAAAAACATCCAACTTACTTTCTAAGCATATGATTGTATGGACCATAATTAATTATAATTTTCGTTTTTGTAACATGTTAGATCACTTAGAGAAGTGCCTAGTTTATTTTTGGTTATGTTCTCATCTTTTGCTTTGAGGCATTGCATTATCAGTATGGTATAGTAGTCCAAATATAGAAGAAGCCCCGTCACAAGATGTAATTTGATGAGCTTTTGAAGTTGCAGTAAGTTCAAACACAATATGTGAGTAAATGAAACTTGTAAATCAGAATATCGTTCTCATTGTTTACTTATTAGGTTTTGACATAGTTGTCACTTGTCAGAATTATGCAGTACAATGCCTAATATCAATAATTAATCAAATTTCCGCAACTGATTGTTTATGGACATTGTTTAGTTTTCAGAAACACCAGATTGGGCATTTATGAAGAAAGCAAGTCAGAAGTTGTCATTTTTGTTCTGTATGGATGACCATTGGGCTCCAATGCATTTGTATGAGGAGGTAAGATATAAACTACTTTCCATTTTGTCAATCAACTCTGCTGCATGAAGATAGTTCTCCAAATACTTTGGCTAAAGAGGTTGTAGTCATAATAGTGTTTATGATGATTAGAACATTGATAATCGGTTTGGAATCTTTAACTTTGTAATACGTAGTCAATAGCATCGAAATAATAAGGAACTATCATTGGTTGGCCTAGTGGTAAATAAAGAAAGGACTGTAGTTTGATAAAGGGCTAAGAGAGAATGAGTTCAATCCATGATAGCTACCTACCAAAGATTTAATATCCTACGAGTTTCATTAACACCAAAATGTAGTAGGGTCAGACGGGTTATCCTGTGAGATTAGTCGAGGAGTACATAAGTTGGCCTGACACTCATGGATATCAAAAAAGGAAAAAAAAAAAAAAAACAGGGTTGAATCCTACTGATTAGCTTTTTCAAGAAAAATGGAGGGGCAAAAGAGCTCAAAATTATTACATTGCAATGGTGATGTCAAGTTCTACTTTGTTAGTTTGTGATCTCTTTTCTCCACAGATTTCCAAGCAGGTCCCTGAAATTGATCTATCAGTTGAAAGGGAGGGCCATTCACATGCATTTTGCTGCTCTGAAGCTGGCTCATTTTATATTGCTCAATATGTGGCAAGCTTGATCAAGAAACATCTCTCAGATTAA

mRNA sequence

ATGGGCCAGCAAGTTCTGCAATCTTTTAGTAAAAGCCCTGTGGAGTTTCGTTTATGCAATGTATCAGGTTACACCAATGAATTACTGGAGATTCATTCCGACGATCCATCATTGCATGTATTGTTCATACCTGGAAATCCAGGGATTATTTCATTTTATAAAGATTTTGTTGAATCACTGTATCAGTTGCTTGGAGGCCGTGTGTCTATAACAGCTATTGGACATATATGTCAAACGAAGAAGGACTGGGAAGGTGGAAGGTTATTTTCGTTACAAGAACAAATTGATCATAAGATTGAATTTGTTAGACAGGAGTTACCAAATAAAGACATCCCGTTACTACTGGTAGGGCATTCTGTTGGATCGTATATCTCCATTGAATTGTACCGTAGGTTCCAGGATCGGGTGGTGTATTGCATTGGATTGCATCCATTTATGATGGTGAACAGACAGTCACGTCAGCAATTTTTTATTGAAAAACTTGCTGGATCCCCACTGCTATCAACTTTGTTTTCTTCTTTTGTTGCGCTGTTGGGTGTATTACCAATTCGAGCTTCAAGTTTTGTGGTGAGAAAAACTATTGGGAAGTCATGGTCCAGAACTGCTTGTGAGGCTGCTTGCTCTCATTTGCTAAAGTACCATAGCATGCGAAATGTGCTATATTTCGCAATGACGGAGTTTGAGAAGTTTTCAGAAACACCAGATTGGGCATTTATGAAGAAAGCAAGTCAGAAGTTGTCATTTTTGTTCTGTATGGATGACCATTGGGCTCCAATGCATTTGTATGAGGAGATTTCCAAGCAGGTCCCTGAAATTGATCTATCAGTTGAAAGGGAGGGCCATTCACATGCATTTTGCTGCTCTGAAGCTGGCTCATTTTATATTGCTCAATATGTGGCAAGCTTGATCAAGAAACATCTCTCAGATTAA

Coding sequence (CDS)

ATGGGCCAGCAAGTTCTGCAATCTTTTAGTAAAAGCCCTGTGGAGTTTCGTTTATGCAATGTATCAGGTTACACCAATGAATTACTGGAGATTCATTCCGACGATCCATCATTGCATGTATTGTTCATACCTGGAAATCCAGGGATTATTTCATTTTATAAAGATTTTGTTGAATCACTGTATCAGTTGCTTGGAGGCCGTGTGTCTATAACAGCTATTGGACATATATGTCAAACGAAGAAGGACTGGGAAGGTGGAAGGTTATTTTCGTTACAAGAACAAATTGATCATAAGATTGAATTTGTTAGACAGGAGTTACCAAATAAAGACATCCCGTTACTACTGGTAGGGCATTCTGTTGGATCGTATATCTCCATTGAATTGTACCGTAGGTTCCAGGATCGGGTGGTGTATTGCATTGGATTGCATCCATTTATGATGGTGAACAGACAGTCACGTCAGCAATTTTTTATTGAAAAACTTGCTGGATCCCCACTGCTATCAACTTTGTTTTCTTCTTTTGTTGCGCTGTTGGGTGTATTACCAATTCGAGCTTCAAGTTTTGTGGTGAGAAAAACTATTGGGAAGTCATGGTCCAGAACTGCTTGTGAGGCTGCTTGCTCTCATTTGCTAAAGTACCATAGCATGCGAAATGTGCTATATTTCGCAATGACGGAGTTTGAGAAGTTTTCAGAAACACCAGATTGGGCATTTATGAAGAAAGCAAGTCAGAAGTTGTCATTTTTGTTCTGTATGGATGACCATTGGGCTCCAATGCATTTGTATGAGGAGATTTCCAAGCAGGTCCCTGAAATTGATCTATCAGTTGAAAGGGAGGGCCATTCACATGCATTTTGCTGCTCTGAAGCTGGCTCATTTTATATTGCTCAATATGTGGCAAGCTTGATCAAGAAACATCTCTCAGATTAA

Protein sequence

MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGVLPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMKKASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVASLIKKHLSD
Homology
BLAST of ClCG04G010350 vs. NCBI nr
Match: XP_038881163.1 (lipid droplet-associated hydrolase [Benincasa hispida])

HSP 1 Score: 598.2 bits (1541), Expect = 4.0e-167
Identity = 294/309 (95.15%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QVLQSFSKS VE RLCNVSGYTNELLEIHSD PSLHVLFIPGNPGIISFYKDFVESL
Sbjct: 34  MGHQVLQSFSKSRVELRLCNVSGYTNELLEIHSDHPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQEL NKDIPLLLVGHSV
Sbjct: 94  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELQNKDIPLLLVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLA S LLSTLFSSFVALLGV
Sbjct: 154 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLARSSLLSTLFSSFVALLGV 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPIRASSFVVRKT GKSWSRTA EAACSHLLKYHSMRNVLY AM EFEKFSETPDWAFMK
Sbjct: 214 LPIRASSFVVRKTFGKSWSRTASEAACSHLLKYHSMRNVLYMAMMEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           KASQKLSFLFCMDDHWAPMH+YEEISKQVPEIDLSVEREGHSHAFCCSEA S YIAQYVA
Sbjct: 274 KASQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGHSHAFCCSEAASLYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. NCBI nr
Match: KAA0025227.1 (lipid droplet-associated hydrolase [Cucumis melo var. makuwa] >TYK07439.1 lipid droplet-associated hydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 592.4 bits (1526), Expect = 2.2e-165
Identity = 285/309 (92.23%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QVLQSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL
Sbjct: 34  MGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPL+LVGHSV
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLILVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVN++SRQQFFIEKLA SPLLST FSSFVALLG+
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLSTFFSSFVALLGI 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLY AMTEFEKFSETPDWAFMK
Sbjct: 214 LPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEI KQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVEREGYSHAFCCSEAASIYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. NCBI nr
Match: XP_008462607.1 (PREDICTED: LOW QUALITY PROTEIN: lipid droplet-associated hydrolase [Cucumis melo])

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-164
Identity = 284/309 (91.91%), Postives = 295/309 (95.47%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QVLQSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL
Sbjct: 34  MGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPL+LVGHSV
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLILVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVN++SRQQFFIEKLA SPLLS  FSSFVALLG+
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLSXFFSSFVALLGI 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLY AMTEFEKFSETPDWAFMK
Sbjct: 214 LPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEI KQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVEREGYSHAFCCSEAASIYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. NCBI nr
Match: XP_011657723.1 (lipid droplet-associated hydrolase isoform X2 [Cucumis sativus])

HSP 1 Score: 584.3 bits (1505), Expect = 6.0e-163
Identity = 282/309 (91.26%), Postives = 294/309 (95.15%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QV QSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESL
Sbjct: 1   MGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPLLLVGHSV
Sbjct: 61  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSV 120

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVNR+SRQQFFIEKL+ SPLLSTLFSSF ALLG 
Sbjct: 121 GSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLSTLFSSFAALLGT 180

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVV+KTIGKSWSRTA EAACSHLLKYHSMRNVLY AMTEF+KFSETPDWAFMK
Sbjct: 181 LPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMK 240

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEISKQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 241 KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVA 300

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 301 SLVKKHLSD 309

BLAST of ClCG04G010350 vs. NCBI nr
Match: XP_004143334.1 (lipid droplet-associated hydrolase isoform X1 [Cucumis sativus] >KGN48293.1 hypothetical protein Csa_004459 [Cucumis sativus])

HSP 1 Score: 584.3 bits (1505), Expect = 6.0e-163
Identity = 282/309 (91.26%), Postives = 294/309 (95.15%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QV QSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESL
Sbjct: 34  MGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPLLLVGHSV
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVNR+SRQQFFIEKL+ SPLLSTLFSSF ALLG 
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLSTLFSSFAALLGT 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVV+KTIGKSWSRTA EAACSHLLKYHSMRNVLY AMTEF+KFSETPDWAFMK
Sbjct: 214 LPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEISKQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. ExPASy Swiss-Prot
Match: Q5HZX7 (Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 8.0e-25
Identity = 82/290 (28.28%), Postives = 133/290 (45.86%), Query Frame = 0

Query: 14  VEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAI 73
           VE ++     +TN   +     P   +  IPGNPG+  FY  F ++LY L+ G   +  I
Sbjct: 21  VETQIIKCGPWTNLFDKQGVSKPKHLIFVIPGNPGLSPFYVPFAKALYSLVKGHFPVWII 80

Query: 74  GH--ICQTKKD------------WEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHS 133
            H   C T KD             E   ++ L  QI+HKI F+R  +P KD+ L+ +GHS
Sbjct: 81  SHAGFCLTPKDKKILTAPQEPNAQEIEDIYGLNGQIEHKIAFLRAHVP-KDVKLIFIGHS 140

Query: 134 VGSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLS----TLFSSFV 193
           VGSYI++ +  R  +  V    +H F++     R          +P L      L+++  
Sbjct: 141 VGSYIALHVMNRAPELPV----VHTFLLFPTIERMSESPNGKFATPFLCRFRYMLYAASY 200

Query: 194 ALLGVLPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPD 253
            +    P    SF+V+K + K   ++  E   + LL+   + N  Y    E     +  D
Sbjct: 201 LIFKPCPEMIKSFIVQKVLEKMSFKS--ELRLTDLLQPFCLANAAYLGGQEMIHVVKRDD 260

Query: 254 WAFMKKASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAF 286
              +K+   KL+F +   D W P++ YE++ +  PE +L +  +G  HAF
Sbjct: 261 -GIIKELLPKLTFYYGKTDGWCPVNYYEDMKRDFPEANLHLCEKGIPHAF 302

BLAST of ClCG04G010350 vs. ExPASy Swiss-Prot
Match: Q8BVA5 (Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.2e-22
Identity = 78/291 (26.80%), Postives = 131/291 (45.02%), Query Frame = 0

Query: 14  VEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAI 73
           VE ++     +TN   +     P   +  IPGNPG  +FY  F ++LY L+  R  +  I
Sbjct: 21  VETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTLMKSRFPVWII 80

Query: 74  GH--ICQTKKD-------------WEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGH 133
            H     T KD              +   ++ L  QI+HKI F+R  +P KD+ L+L+GH
Sbjct: 81  SHAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVP-KDVKLILIGH 140

Query: 134 SVGSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLS----TLFSSF 193
           SVG+Y+++ + +R  +  V     H F++     R          +P L      L+++ 
Sbjct: 141 SVGTYMTLHVMKRVLELPV----AHAFLLFPTIERMSESPNGKFATPFLCQFRYLLYATS 200

Query: 194 VALLGVLPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETP 253
             L    P    SF+++K +G+       E   + +L+   + N  Y    E  +  +  
Sbjct: 201 YLLFKPCPEVIKSFIIQKLMGQ--MNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRD 260

Query: 254 DWAFMKKASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAF 286
           D   +K+   KL F +   D W P+  YE++ K  PE ++ +  +G  HAF
Sbjct: 261 D-DIIKEFLPKLKFYYGKTDGWCPVKYYEDMKKDFPEGNIYLCEKGIPHAF 303

BLAST of ClCG04G010350 vs. ExPASy Swiss-Prot
Match: Q54LL8 (Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0286581 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.1e-21
Identity = 81/271 (29.89%), Postives = 126/271 (46.49%), Query Frame = 0

Query: 27  ELLEIHSDDPS-LHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAIGHICQTKKDWEG 86
           E++   S  PS + ++ I GNPGI SFY++FV+ L      +  I  +GHI    K    
Sbjct: 21  EIIYTKSQTPSNIKIIVIAGNPGIESFYQEFVKVLNLSFNSKYDIYGVGHIGHCGK--IE 80

Query: 87  GRLFSLQEQIDHKIEFVRQELPN-------KDIPLLLVGHSVGSYISIELYRRFQDRVVY 146
            + FS++EQI HK  F+   L N       KDI  +L+GHSVGSYIS+++  RF ++  +
Sbjct: 81  NKTFSVEEQIKHKELFLEYLLKNKYGDKDRKDIKFILIGHSVGSYISLKVVSRFSEKFEF 140

Query: 147 --CIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGVLPIRASSFVVRKTIGK 206
              + L P    N       FI+ +          S+F   L  +P    S V++  +  
Sbjct: 141 LSVVNLFP-TFKNLYDGLSPFIKMVVMRESTRNGLSTF---LHYIPSIVVSNVLKWILPS 200

Query: 207 SWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPD--WAFMKKASQKLSFLFCMDD 266
             SR A ++     + Y+S  N+LY A TE E   E  D   +       +L F++   D
Sbjct: 201 DESRIAVQSK----INYYSALNILYMAYTETEDIKEIDDECHSVFNSRLNQLLFIYGQTD 260

Query: 267 HWAPMHLYEEISKQVPEIDLSVEREGHSHAF 286
            + P   Y+E+ +  P  ++        HAF
Sbjct: 261 SYTPKSFYDEMKQLYPAGNIEYSSSYVPHAF 281

BLAST of ClCG04G010350 vs. ExPASy Swiss-Prot
Match: Q5F477 (Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-21
Identity = 78/268 (29.10%), Postives = 130/268 (48.51%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAIGHICQTK-------------KD 95
           P L  + IPGNPG+  +Y+ F+++LY  L  +  +  + H    K             K+
Sbjct: 43  PRLLFMIIPGNPGLAGYYRTFIKALYCGLNQQYPVWVVSHAGHCKPPSGMEMIEDTDIKE 102

Query: 96  WEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGSYISIELYRRFQD-RVVYCIG 155
            E   +F L  Q++HK+ F+++ + +KDI L+L+ HS+G YI++E+ +R  + +V+  + 
Sbjct: 103 LED--VFGLNGQVEHKLNFLKKNV-SKDIKLVLIAHSIGCYITLEMMKRASELQVLRSVL 162

Query: 156 LHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSF---VALLGVLPIRASSFVVRKTI-GKS 215
           L P +    QS Q   +     +PLL  L  +    V LL  LP    + +VR  + G  
Sbjct: 163 LFPTIERMAQSPQGKLM-----TPLLCKLRYALYMPVYLLSFLPEGVKASLVRFALRGMK 222

Query: 216 WSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMKKASQKLSFLFCMDDHWA 275
               +      +L     + N+LY A  E  K  E  D   +K+  +KL F +   D W 
Sbjct: 223 TCDESSITTSVNLFSVDCIANILYMASQEMMKVVER-DSTTIKQNLKKLIFYYGTGDSWC 282

Query: 276 PMHLYEEISKQVPEIDLSVEREGHSHAF 286
           P + Y+EI    P+ D+ +  +G  HAF
Sbjct: 283 PQNYYDEIKMDFPDGDIRLCEKGLRHAF 301

BLAST of ClCG04G010350 vs. ExPASy Swiss-Prot
Match: Q9H6V9 (Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 7.0e-21
Identity = 81/292 (27.74%), Postives = 130/292 (44.52%), Query Frame = 0

Query: 36  PSLHVLFIPGNPGIISFYKDFVESLYQLLGGRVSITAIGH-----------ICQTKKD-- 95
           P L +  IPGNPG  +FY  F ++LY L   R  +  I H           I  T +D  
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRRFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 96  -WEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGSYISIELYRRFQDRVVYCIG 155
             E   ++ L  QI+HK+ F+R  +P KD+ L+L+GHS+GSY ++++ +R  +  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSIGSYFTLQMLKRVPELPV---- 162

Query: 156 LHPFMMVNRQSRQQFFIEKLAGSPLLS----TLFSSFVALLGVLPIRASSFVVRKTIGKS 215
           +  F++     R          +PLL      L+ +   LL   P    S ++R+  G  
Sbjct: 163 IRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRR--GLQ 222

Query: 216 WSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMKKASQKLSFLFCMDDHWA 275
                 E +  ++L+   + N  Y    E  +  +  D   +K+   KL+F +   D W 
Sbjct: 223 VMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDD-ETIKEHLCKLTFYYGTIDPWC 282

Query: 276 PMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVASLIKKHLSD 310
           P   YE+I K  PE D+ +  +   HAF        +  Q +A +I   L D
Sbjct: 283 PKEYYEDIKKDFPEGDIRLCEKNIPHAFIT------HFNQEMADMIADSLKD 320

BLAST of ClCG04G010350 vs. ExPASy TrEMBL
Match: A0A5A7SKD4 (Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001690 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 285/309 (92.23%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QVLQSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL
Sbjct: 34  MGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPL+LVGHSV
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLILVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVN++SRQQFFIEKLA SPLLST FSSFVALLG+
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLSTFFSSFVALLGI 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLY AMTEFEKFSETPDWAFMK
Sbjct: 214 LPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEI KQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVEREGYSHAFCCSEAASIYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. ExPASy TrEMBL
Match: A0A1S3CHU9 (LOW QUALITY PROTEIN: lipid droplet-associated hydrolase OS=Cucumis melo OX=3656 GN=LOC103500925 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.3e-165
Identity = 284/309 (91.91%), Postives = 295/309 (95.47%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QVLQSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL
Sbjct: 34  MGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPL+LVGHSV
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLILVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVN++SRQQFFIEKLA SPLLS  FSSFVALLG+
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLSXFFSSFVALLGI 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLY AMTEFEKFSETPDWAFMK
Sbjct: 214 LPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFEKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEI KQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVEREGYSHAFCCSEAASIYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. ExPASy TrEMBL
Match: A0A0A0KFF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 2.9e-163
Identity = 282/309 (91.26%), Postives = 294/309 (95.15%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MG QV QSFSK  VEFRLCNVSG+TNELLEIHSDDPSLHVLFIPGNPGIISFYKDF+ESL
Sbjct: 34  MGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESL 93

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGG VSITAIGHICQTKKDWEGGRLFSLQEQIDHK+EFVRQEL NKDIPLLLVGHSV
Sbjct: 94  YQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQNKDIPLLLVGHSV 153

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIEL+RRFQDR VYCIGLHPFMMVNR+SRQQFFIEKL+ SPLLSTLFSSF ALLG 
Sbjct: 154 GSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLSTLFSSFAALLGT 213

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LPI+ASSFVV+KTIGKSWSRTA EAACSHLLKYHSMRNVLY AMTEF+KFSETPDWAFMK
Sbjct: 214 LPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFDKFSETPDWAFMK 273

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K SQKLSFLFCMDDHWAPMH+YEEISKQVPEIDLSVEREG+SHAFCCSEA S YIAQYVA
Sbjct: 274 KVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCSEAASMYIAQYVA 333

Query: 301 SLIKKHLSD 310
           SL+KKHLSD
Sbjct: 334 SLVKKHLSD 342

BLAST of ClCG04G010350 vs. ExPASy TrEMBL
Match: A0A6J1EDI9 (lipid droplet-associated hydrolase-like OS=Cucurbita moschata OX=3662 GN=LOC111433317 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.7e-153
Identity = 271/310 (87.42%), Postives = 284/310 (91.61%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           M Q+ LQ FS+  VEFRLCNVSGYTNELLEIHSD+PSLHVLFIPGNPGIISFYKDFVESL
Sbjct: 1   MDQEALQLFSRRRVEFRLCNVSGYTNELLEIHSDNPSLHVLFIPGNPGIISFYKDFVESL 60

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFV+QEL NK+IPLLLVGHS+
Sbjct: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVKQELQNKEIPLLLVGHSI 120

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GS ISIEL R F DRVVYCIGL+PFMMVNRQSR Q  IEKLAGSPLLS LFSSF ALLGV
Sbjct: 121 GSPISIELCRWFLDRVVYCIGLYPFMMVNRQSRWQLLIEKLAGSPLLSALFSSFAALLGV 180

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LP+RASSFVV+KTIGKSWSRTA EAACSHLLKYH+MRNVLY AMTEFEK SETPDW FMK
Sbjct: 181 LPVRASSFVVKKTIGKSWSRTASEAACSHLLKYHTMRNVLYMAMTEFEKLSETPDWKFMK 240

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVER-EGHSHAFCCSEAGSFYIAQYV 300
           KASQKLSFLFC DDHWAPMH++EEI KQVPEID SVER EGH HAFCCSEAGS +IAQYV
Sbjct: 241 KASQKLSFLFCKDDHWAPMHVFEEICKQVPEIDASVEREEGHKHAFCCSEAGSLHIAQYV 300

Query: 301 ASLIKKHLSD 310
           ASLIK HLSD
Sbjct: 301 ASLIKNHLSD 310

BLAST of ClCG04G010350 vs. ExPASy TrEMBL
Match: A0A6J1I921 (lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471128 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.7e-153
Identity = 263/309 (85.11%), Postives = 287/309 (92.88%), Query Frame = 0

Query: 1   MGQQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESL 60
           MGQ+VL  FS+   +FRLCNVSGYTNELLEIH+DDPSLHVLFIPGNPGI+SFYK FVESL
Sbjct: 43  MGQEVLNPFSRWRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPGIVSFYKYFVESL 102

Query: 61  YQLLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSV 120
           YQLLGGRVSITAIGH+ QT KDWEGGRLFSLQEQIDHKIEFVRQEL +KDIPLLLVGHS+
Sbjct: 103 YQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQSKDIPLLLVGHSI 162

Query: 121 GSYISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGV 180
           GSYISIELYR+FQDRVVYCIGLHPFMMVNRQSRQQF I KLAGS LLSTLFSSFVALLGV
Sbjct: 163 GSYISIELYRKFQDRVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLSTLFSSFVALLGV 222

Query: 181 LPIRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMK 240
           LP R SSFVV KTIGKSWSRTA EAAC++LLKYH MRN+L+ A+TEFEK SETPDW FMK
Sbjct: 223 LPSRVSSFVVGKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFEKLSETPDWGFMK 282

Query: 241 KASQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVA 300
           K+S+KLSFLFCMDDHWAPMH++EEISKQ+PE+D+SVEREGH+HAFCCSEAGS YIAQ+VA
Sbjct: 283 KSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREGHTHAFCCSEAGSLYIAQHVA 342

Query: 301 SLIKKHLSD 310
           SLIK H+SD
Sbjct: 343 SLIKNHISD 351

BLAST of ClCG04G010350 vs. TAIR 10
Match: AT3G11620.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.2e-106
Identity = 174/306 (56.86%), Postives = 232/306 (75.82%), Query Frame = 0

Query: 3   QQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQ 62
           Q  L + +K  V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDF+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHS+GS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 YISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGVLP 182
           YIS+EL ++F D+VVYCIGL+PF+ +N+QS +Q  I KLA S +LS   S  +A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 IRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMKKA 242
           + A+  +V K+IG SWS TA +A C+HL +YH+MRNVL+ A +EF + +  PDW FM++ 
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMREN 243

Query: 243 SQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVASL 302
             KL+FLF +DDHW P+ L+EEISKQ P   LS+EREGH+H FCC+ AGS ++AQ+VA+L
Sbjct: 244 QSKLAFLFGIDDHWGPLQLFEEISKQAPHTSLSIEREGHTHGFCCTVAGSAWVAQHVATL 303

Query: 303 IKKHLS 309
           IK   S
Sbjct: 304 IKNRFS 309

BLAST of ClCG04G010350 vs. TAIR 10
Match: AT3G11620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.2e-106
Identity = 174/306 (56.86%), Postives = 232/306 (75.82%), Query Frame = 0

Query: 3   QQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQ 62
           Q  L + +K  V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDF+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHS+GS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 YISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGVLP 182
           YIS+EL ++F D+VVYCIGL+PF+ +N+QS +Q  I KLA S +LS   S  +A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 IRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEKFSETPDWAFMKKA 242
           + A+  +V K+IG SWS TA +A C+HL +YH+MRNVL+ A +EF + +  PDW FM++ 
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMREN 243

Query: 243 SQKLSFLFCMDDHWAPMHLYEEISKQVPEIDLSVEREGHSHAFCCSEAGSFYIAQYVASL 302
             KL+FLF +DDHW P+ L+EEISKQ P   LS+EREGH+H FCC+ AGS ++AQ+VA+L
Sbjct: 244 QSKLAFLFGIDDHWGPLQLFEEISKQAPHTSLSIEREGHTHGFCCTVAGSAWVAQHVATL 303

Query: 303 IKKHLS 309
           IK   S
Sbjct: 304 IKNRFS 309

BLAST of ClCG04G010350 vs. TAIR 10
Match: AT3G11620.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.3e-75
Identity = 129/227 (56.83%), Postives = 173/227 (76.21%), Query Frame = 0

Query: 3   QQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQ 62
           Q  L + +K  V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDF+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHS+GS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 YISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGVLP 182
           YIS+EL ++F D+VVYCIGL+PF+ +N+QS +Q  I KLA S +LS   S  +A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 IRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEK 230
           + A+  +V K+IG SWS TA +A C+HL +YH+MRNVL+ A +EF +
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRE 230

BLAST of ClCG04G010350 vs. TAIR 10
Match: AT3G11620.4 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.3e-75
Identity = 129/227 (56.83%), Postives = 173/227 (76.21%), Query Frame = 0

Query: 3   QQVLQSFSKSPVEFRLCNVSGYTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFVESLYQ 62
           Q  L + +K  V+ RLC VSG   E++EI +++P+ HVLFIPGNPG++SFYKDF+ESLY+
Sbjct: 4   QNKLMNETKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYE 63

Query: 63  LLGGRVSITAIGHICQTKKDWEGGRLFSLQEQIDHKIEFVRQELPNKDIPLLLVGHSVGS 122
            LGG  S+ AIG I  T KDWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHS+GS
Sbjct: 64  FLGGNASVIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGS 123

Query: 123 YISIELYRRFQDRVVYCIGLHPFMMVNRQSRQQFFIEKLAGSPLLSTLFSSFVALLGVLP 182
           YIS+EL ++F D+VVYCIGL+PF+ +N+QS +Q  I KLA S +LS   S  +A L +LP
Sbjct: 124 YISLELLKKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLP 183

Query: 183 IRASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYFAMTEFEK 230
           + A+  +V K+IG SWS TA +A C+HL +YH+MRNVL+ A +EF +
Sbjct: 184 MSAARLLVSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRE 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881163.14.0e-16795.15lipid droplet-associated hydrolase [Benincasa hispida][more]
KAA0025227.12.2e-16592.23lipid droplet-associated hydrolase [Cucumis melo var. makuwa] >TYK07439.1 lipid ... [more]
XP_008462607.11.1e-16491.91PREDICTED: LOW QUALITY PROTEIN: lipid droplet-associated hydrolase [Cucumis melo... [more]
XP_011657723.16.0e-16391.26lipid droplet-associated hydrolase isoform X2 [Cucumis sativus][more]
XP_004143334.16.0e-16391.26lipid droplet-associated hydrolase isoform X1 [Cucumis sativus] >KGN48293.1 hypo... [more]
Match NameE-valueIdentityDescription
Q5HZX78.0e-2528.28Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV... [more]
Q8BVA52.2e-2226.80Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1[more]
Q54LL81.1e-2129.89Lipid droplet-associated hydrolase OS=Dictyostelium discoideum OX=44689 GN=DDB_G... [more]
Q5F4771.8e-2129.10Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2[more]
Q9H6V97.0e-2127.74Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SKD41.1e-16592.23Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CHU95.3e-16591.91LOW QUALITY PROTEIN: lipid droplet-associated hydrolase OS=Cucumis melo OX=3656 ... [more]
A0A0A0KFF62.9e-16391.26Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1[more]
A0A6J1EDI92.7e-15387.42lipid droplet-associated hydrolase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I9214.7e-15385.11lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT3G11620.23.2e-10656.86alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.13.2e-10656.86alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.32.3e-7556.83alpha/beta-Hydrolases superfamily protein [more]
AT3G11620.42.3e-7556.83alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 18..304
e-value: 6.2E-12
score: 47.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 30..288
IPR019363Lipid droplet-associated hydrolasePFAMPF10230LIDHydrolasecoord: 38..286
e-value: 1.3E-63
score: 215.1
IPR019363Lipid droplet-associated hydrolasePANTHERPTHR13390LIPASEcoord: 7..307
NoneNo IPR availablePANTHERPTHR13390:SF2BNAA05G27450D PROTEINcoord: 7..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG04G010350.2ClCG04G010350.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019915 lipid storage
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005811 lipid droplet
molecular_function GO:0016298 lipase activity