ClCG04G003250 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG04G003250
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	CG_Chr04: 11999650 .. 12000313 (+)
RNA-Seq Expression	ClCG04G003250
Synteny	ClCG04G003250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGATTGGAATCGAGTGAAAGTTTTGGGTATAGATTTTTATGGGACAATGTTCTTACCAGTTCCAATGAGTGGGGCTCTTGATTTTGTTGCAATTAAGGTAGTTTCCATAGAAGATGCTCATTCCCTCATGACAGAAGAAACTATTTTGCTCAATTTTAAGGGATGTCTTGAAATTTTCCAATGCCTTGGAAGTGGTATCGGATTTGAAGGGGATTCTTTAGATTCTATTTCTTACAATCTTGCATTAGAATATGCTGCAACAGGTAGTTTAGCAGACTTGATTAGGAGAAGAGAGAAATTGCCCGACACCGAAGTCAAAGAGTATCTCAGAATGATACTGAGATGCCTTTATCAAATACATGAACAAGGGTATGTACATGGCGATATCAAACCAGATAATATTCTTGTGTTCCCCGATGATAATGGCAATAGTTGAAGATAGCTGCTTTGGGCTTTCCAAGAAATGCGATGAGTTTGAGGAAGTGGTGATAACCACTAATGCCAAGCCAAGATTTCAAGGAACACCAAGATATATGTCACCAGAATCTATTCTTTTTGGTAAGATCAACACTTCACTTGATATCTGGTCTCTGAGTTGCATTTTGATTGAGATGATTTCTGGAAAGCACGTGTGGAGTGATTGTAAAAGTGTGGGATGA

mRNA sequence

ATGTCAGATTGGAATCGAGTGAAAGTTTTGGGTATAGATTTTTATGGGACAATGTTCTTACCAGTTCCAATGAGTGGGGCTCTTGATTTTGTTGCAATTAAGGTAGTTTCCATAGAAGATGCTCATTCCCTCATGACAGAAGAAACTATTTTGCTCAATTTTAAGGGATGTCTTGAAATTTTCCAATGCCTTGGAAGTGGTATCGGATTTGAAGGGGATTCTTTAGATTCTATTTCTTACAATCTTGCATTAGAATATGCTGCAACAGGTAGTTTAGCAGACTTGATTAGGAGAAGAGAGAAATTGCCCGACACCGAAGTCAAAGAGTATCTCAGAATGATACTGAGATGCCTTTATCAAATACATGAACAAGGGTATGTACATGGCGATATCAAACCAGATAATATTCTTGTGTTCCCCGATGATAATGGCAATAATAGCTGCTTTGGGCTTTCCAAGAAATGCGATGAGTTTGAGGAAGTGGTGATAACCACTAATGCCAAGCCAAGATTTCAAGGAACACCAAGATATATGTCACCAGAATCTATTCTTTTTGGTAAGATCAACACTTCACTTGATATCTGGTCTCTGAGTTGCATTTTGATTGAGATGATTTCTGGAAAGCACGTGTGGAGTGATTGTAAAAGTGTGGGATGA

Coding sequence (CDS)

ATGTCAGATTGGAATCGAGTGAAAGTTTTGGGTATAGATTTTTATGGGACAATGTTCTTACCAGTTCCAATGAGTGGGGCTCTTGATTTTGTTGCAATTAAGGTAGTTTCCATAGAAGATGCTCATTCCCTCATGACAGAAGAAACTATTTTGCTCAATTTTAAGGGATGTCTTGAAATTTTCCAATGCCTTGGAAGTGGTATCGGATTTGAAGGGGATTCTTTAGATTCTATTTCTTACAATCTTGCATTAGAATATGCTGCAACAGGTAGTTTAGCAGACTTGATTAGGAGAAGAGAGAAATTGCCCGACACCGAAGTCAAAGAGTATCTCAGAATGATACTGAGATGCCTTTATCAAATACATGAACAAGGGTATGTACATGGCGATATCAAACCAGATAATATTCTTGTGTTCCCCGATGATAATGGCAATAATAGCTGCTTTGGGCTTTCCAAGAAATGCGATGAGTTTGAGGAAGTGGTGATAACCACTAATGCCAAGCCAAGATTTCAAGGAACACCAAGATATATGTCACCAGAATCTATTCTTTTTGGTAAGATCAACACTTCACTTGATATCTGGTCTCTGAGTTGCATTTTGATTGAGATGATTTCTGGAAAGCACGTGTGGAGTGATTGTAAAAGTGTGGGATGA

Protein sequence

MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEIFQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQIHEQGYVHGDIKPDNILVFPDDNGNNSCFGLSKKCDEFEEVVITTNAKPRFQGTPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG

Homology

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BLAST of ClCG04G003250 vs. NCBI nr
Match: XP_038896352.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 342.0 bits (876), Expect = 3.7e-90
Identity = 170/222 (76.58%), Postives = 188/222 (84.68%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           MS+WNRVKVLG   +G +FL VP S  LDFVA+KVVSIE ++SLM EETILLNF GC EI
Sbjct: 1   MSNWNRVKVLGKGSHGIVFLAVPTSAPLDFVALKVVSIEYSYSLMIEETILLNFNGCPEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            Q +GSGI FEGDSLD+++YNLALEYA  GSL DLIRRREKLP+ EVKEYLRMILR L  
Sbjct: 61  IQRVGSGICFEGDSLDTVTYNLALEYATGGSLTDLIRRREKLPEVEVKEYLRMILRGLCC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPRFQGTPR 180
           IHE+GYVHGDIKPDNILVFPDD G        FGLSKKCDEFEEVVI+TNAKPRF+GTP+
Sbjct: 121 IHERGYVHGDIKPDNILVFPDDTGKMKLKIADFGLSKKCDEFEEVVISTNAKPRFRGTPK 180

Query: 181 YMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG 219
           YMSPESILFG INTSLDIWSL CILIEM+SGK VWSDCK++G
Sbjct: 181 YMSPESILFGMINTSLDIWSLGCILIEMVSGKQVWSDCKTLG 222

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BLAST of ClCG04G003250 vs. NCBI nr
Match: XP_038896344.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 341.3 bits (874), Expect = 6.3e-90
Identity = 169/222 (76.13%), Postives = 189/222 (85.14%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           MS+WNRVKVLG   +GT+FL +  S  LDFVA+KVVS+E A+SLM EETILLNF GC EI
Sbjct: 1   MSNWNRVKVLGKGSHGTVFLAMSTSAPLDFVALKVVSVEYAYSLMIEETILLNFNGCSEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            Q +GSGI FEGDSLD+++YNLALEYA  G+L DLIRRREKLP+ EVKEYLRMILR L  
Sbjct: 61  IQRVGSGICFEGDSLDTVTYNLALEYAVGGNLTDLIRRREKLPEVEVKEYLRMILRGLCC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPRFQGTPR 180
           IHE+GYVHGDIKPDNILVFPDD G        FGLSKKCDEFEEVVI+TNAKPRF+GTP+
Sbjct: 121 IHERGYVHGDIKPDNILVFPDDTGKVKLKIADFGLSKKCDEFEEVVISTNAKPRFRGTPK 180

Query: 181 YMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG 219
           YMSPESILFGKINTSLDIWSL C+LIEMISGK VWSDCK++G
Sbjct: 181 YMSPESILFGKINTSLDIWSLGCVLIEMISGKPVWSDCKTLG 222

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BLAST of ClCG04G003250 vs. NCBI nr
Match: XP_038896328.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 323.6 bits (828), Expect = 1.4e-84
Identity = 166/222 (74.77%), Postives = 185/222 (83.33%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           MS+WNRVKVLG   +GT+FL VP+S   DFVA+KVVS+E A+SLM EETILLNF GC EI
Sbjct: 1   MSNWNRVKVLGRGSHGTVFLAVPISAPFDFVALKVVSVEYAYSLMIEETILLNFNGCPEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            Q +GSGI FEGDSLD+++YNLALEYAA GSL DLIRRREKLP+ EVKEYL+MILR L  
Sbjct: 61  IQRVGSGICFEGDSLDTVTYNLALEYAAGGSLTDLIRRREKLPEVEVKEYLKMILRGLCC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPRFQGTPR 180
           IHE+GYVHGDIKPDNILVF DD G        FGLSKKCDEFEEV+I+TNAKPRF+GTP+
Sbjct: 121 IHERGYVHGDIKPDNILVFLDDIGKVKLKIADFGLSKKCDEFEEVMISTNAKPRFRGTPK 180

Query: 181 YMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG 219
           YMSPESILFGKINTSLDIWSL CILIEMISGK     C+S G
Sbjct: 181 YMSPESILFGKINTSLDIWSLGCILIEMISGK---PSCESNG 219

[Close]

BLAST of ClCG04G003250 vs. NCBI nr
Match: XP_038896342.1 (mitogen-activated protein kinase kinase kinase 3-like [Benincasa hispida])

HSP 1 Score: 321.6 bits (823), Expect = 5.1e-84
Identity = 160/222 (72.07%), Postives = 181/222 (81.53%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           MS+WN+VKVLG   +GT+FL  P S  LDFVA+KVV +E  +SLM EETILLNF GC EI
Sbjct: 1   MSNWNQVKVLGKGSHGTVFLAGPTSAPLDFVALKVVLVEYVYSLMIEETILLNFNGCPEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            Q +GSGI FEGDSLD+++YNL LEY A GSL DLIRRREK P+ EVKEYL+MILR L  
Sbjct: 61  IQRVGSGICFEGDSLDTVTYNLTLEYDAGGSLIDLIRRREKFPEVEVKEYLKMILRGLCC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPRFQGTPR 180
           IHE+GYVHGDIK DNILVFPDD G        FGLSKKCDEFEEV I+TNAKPRF+GTP+
Sbjct: 121 IHERGYVHGDIKVDNILVFPDDTGKVKLKIANFGLSKKCDEFEEVAISTNAKPRFRGTPK 180

Query: 181 YMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG 219
           Y+SPESILFGKINTSLDIWSL C+LIEMI GK  WSDCK++G
Sbjct: 181 YISPESILFGKINTSLDIWSLGCVLIEMIYGKPEWSDCKTLG 222

[Close]

BLAST of ClCG04G003250 vs. NCBI nr
Match: XP_038896341.1 (mitogen-activated protein kinase kinase 1-like [Benincasa hispida])

HSP 1 Score: 276.2 bits (705), Expect = 2.5e-70
Identity = 138/191 (72.25%), Postives = 158/191 (82.72%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           MS+WN+VKVLG   +GT+FL VP S  LDFVA+KVV +E A+SLM EETILLNF GC EI
Sbjct: 1   MSNWNQVKVLGKGSHGTVFLAVPTSAPLDFVALKVVLVEYAYSLMIEETILLNFNGCPEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            QC+G GI FEGDSLD+++Y+LALEYAA GSL DLIRRREKLP+ EVK+YL+MILR L  
Sbjct: 61  IQCVGGGIYFEGDSLDTVTYSLALEYAARGSLTDLIRRREKLPEVEVKKYLKMILRGLCC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPRFQGTPR 180
           IHE+GYVHGDIK DNILVFPDD G        FGLSKKCDEFEEV+I+TN KPRF+GTP+
Sbjct: 121 IHERGYVHGDIKVDNILVFPDDTGKVKLKIANFGLSKKCDEFEEVMISTNVKPRFRGTPK 180

Query: 181 YMSPESILFGK 188
           YMSPESILF K
Sbjct: 181 YMSPESILFIK 191

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BLAST of ClCG04G003250 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-26
Identity = 72/216 (33.33%), Postives = 115/216 (53.24%), Query Frame = 0


Query: 3   DWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAH---SLMTEETILLNFKGCLE 62
           +W R + +G   + T+        + DF A+  V   DA+   SL  E+++L +   C E
Sbjct: 2   EWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPE 61

Query: 63  IFQCLGSGIGFE-GDSLDSISYNLALEYAATGSLADLIRR--REKLPDTEVKEYLRMILR 122
           I +C G     E G+ +    +NL LEYA+ GSLA  +++   E LP++ V+ +   +LR
Sbjct: 62  IIRCYGEDSTVENGEEM----HNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 121

Query: 123 CLYQIHEQGYVHGDIKPDNILVFPDDNGNNSCFGLSKKCDEFEEVVITTNAKPRFQGTPR 182
            L  IH +G+ H DIK  NIL+F D +   + FGL+ + D     +         +GTP 
Sbjct: 122 GLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVD---GDLTALRKSVEIRGTPL 181

Query: 183 YMSPESILFGKINTSLDIWSLSCILIEMISGKHVWS 213
           YM+PE +   +  ++ D+W+L C ++EM SGK  WS
Sbjct: 182 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWS 210

[Close]

BLAST of ClCG04G003250 vs. ExPASy Swiss-Prot
Match: P28829 (Protein kinase byr2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=byr2 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.3e-23
Identity = 68/214 (31.78%), Postives = 109/214 (50.93%), Query Frame = 0


Query: 4   WNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLN-FKGCLEIFQ 63
           W R  ++G   +G ++L +  S   + +A+K V ++           LL+   G + + Q
Sbjct: 394 WIRGALIGSGSFGQVYLGMNASSG-ELMAVKQVILDSVSESKDRHAKLLDALAGEIALLQ 453

Query: 64  CLGSG--IGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 123
            L     + + G +L+S   N+ LEY   GS+A L+       +T VK +++  L+ L  
Sbjct: 454 ELSHEHIVQYLGSNLNSDHLNIFLEYVPGGSVAGLLTMYGSFEETLVKNFIKQTLKGLEY 513

Query: 124 IHEQGYVHGDIKPDNILVFPDDNGNNSCFGLSKKCDEFEEVVITTNAKPRFQGTPRYMSP 183
           +H +G VH DIK  NILV        S FG+SKK +       T  A+P FQG+  +M+P
Sbjct: 514 LHSRGIVHRDIKGANILVDNKGKIKISDFGISKKLELNSTSTKTGGARPSFQGSSFWMAP 573

Query: 184 ESILFGKINTSLDIWSLSCILIEMISGKHVWSDC 215
           E +         DIWSL C++IEM++ KH + +C
Sbjct: 574 EVVKQTMHTEKTDIWSLGCLVIEMLTSKHPYPNC 606

[Close]

BLAST of ClCG04G003250 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 2.1e-19
Identity = 64/212 (30.19%), Postives = 108/212 (50.94%), Query Frame = 0


Query: 4   WNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEIFQC 63
           W + ++LG   YG+++L +      +  A+K + I D +S    + ++L+F   +E+ + 
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTG-ELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRS 229

Query: 64  L--GSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQI 123
           L   + + + G SLD    ++ LEY   GS++ L+ +     +  +K Y + IL+ L  +
Sbjct: 230 LRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLSFL 289

Query: 124 HEQGYVHGDIKPDNILVFPDDNGNNSCFGLSKKCDEFEEVVITTNAKPRFQGTPRYMSPE 183
           H    +H DIK  NIL+        S FG SK    F  +V    +    QGTP +M+PE
Sbjct: 290 HANSIIHRDIKGANILIDTKGIVKLSDFGCSK---SFSGIVSQFKS---MQGTPYWMAPE 349

Query: 184 SILFGKINTSLDIWSLSCILIEMISGKHVWSD 214
            I       S DIWSL C+++EM + +  WS+
Sbjct: 350 VIKQTGHGRSSDIWSLGCVIVEMATAQPPWSN 374

[Close]

BLAST of ClCG04G003250 vs. ExPASy Swiss-Prot
Match: Q8T2I8 (Serine/threonine-protein kinase sepA OS=Dictyostelium discoideum OX=44689 GN=sepA PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.5e-19
Identity = 66/220 (30.00%), Postives = 110/220 (50.00%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSI-----EDAHSLMTEETIL--LN 60
           + ++N   V+G   +GT++  + +    DFVAIK +++     +    +M E  +L  LN
Sbjct: 15  VGNYNLGVVIGKGGFGTVYQGLDIEDG-DFVAIKQINLTKIPKDQLQGIMNEIDLLKNLN 74

Query: 61  FKGCLEIFQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRM 120
               ++  + + +      D+L      + LEY   GSL+ +I++  K P+T V  Y+R 
Sbjct: 75  HANIVKYIKYVKT-----KDNL-----YIVLEYVENGSLSGIIKKFGKFPETLVCVYIRQ 134

Query: 121 ILRCLYQIHEQGYVHGDIKPDNILVFPDDNGNNSCFGLSKKCDEFEEVVITTNAKPRFQG 180
           +L  L  +HEQG VH DIK  NIL   +     + FG++ K D+     +         G
Sbjct: 135 VLEGLVYLHEQGVVHRDIKGANILTTKEGKIKLADFGVATKFDDTSAAAVV--------G 194

Query: 181 TPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSD 214
           TP +M+PE I      T  DIWS+ C +IE+++G   + D
Sbjct: 195 TPYWMAPEIIELNGATTKSDIWSVGCTVIELLTGSPPYYD 215

[Close]

BLAST of ClCG04G003250 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.3e-18
Identity = 71/221 (32.13%), Postives = 114/221 (51.58%), Query Frame = 0


Query: 4   WNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSI---------EDAHSLMTEETI-LLN 63
           W + +++G   +G +++ + +    + +AIK VSI           AH    EE + LL 
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSG-ELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLK 139

Query: 64  FKGCLEIFQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRM 123
                 I + LG+       + ++ S N+ LE+   GS++ L+ +    P++ ++ Y + 
Sbjct: 140 NLSHPNIVRYLGT-------AREAGSLNILLEFVPGGSISSLLGKFGSFPESVIRMYTKQ 199

Query: 124 ILRCLYQIHEQGYVHGDIKPDNILVFPDDNG--NNSCFGLSKKCDEFEEVVITTNAKPRF 183
           +L  L  +H+ G +H DIK  NILV  D+ G    + FG SKK     E+   T AK   
Sbjct: 200 LLLGLEYLHKNGIMHRDIKGANILV--DNKGCIKLADFGASKK---VVELATMTGAK-SM 259

Query: 184 QGTPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWS 213
           +GTP +M+PE IL    + S DIWS+ C +IEM +GK  WS
Sbjct: 260 KGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWS 286

[Close]

BLAST of ClCG04G003250 vs. ExPASy TrEMBL
Match: A0A6J1DHA9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111020049 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.7e-53
Identity = 113/226 (50.00%), Postives = 149/226 (65.93%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           +  W RV +LG    G +FL  P  G + +VA+K   I  + SL+ E+ IL +F GC EI
Sbjct: 12  LGTWTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSEI 71

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            QC+G  I  E  SL    YNL LEYAA G+L +LI++++ LP+ EVKEYL+MILR L  
Sbjct: 72  IQCIGDEITLEESSL---CYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLSC 131

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKC-DEFEEVV---ITTNAKPRFQ 180
           IH++G+VHGD+KP NIL FP  +G        FGLSK+C  +  EVV   +T  +KP+F+
Sbjct: 132 IHDKGFVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKFK 191

Query: 181 GTPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG 219
           GTP YMSPES+ FG I+   DIWSL CIL+EMI+GK VW  C++ G
Sbjct: 192 GTPIYMSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSG 234

[Close]

BLAST of ClCG04G003250 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 8.6e-53
Identity = 112/218 (51.38%), Postives = 148/218 (67.89%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           M++W RV  LG   YG +FL  P + +L + A+KV  ++   SL+ E+ IL +F G  EI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            QC G  +  EGDS DS  Y+L LEYAA G+LADLIR+++KL +  VKEYL+MILR L  
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPRFQGTPR 180
           IH+ G+VH D+KP NIL FP  +G        FGLS++C++  E     N K +F+GTP 
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE----CNFKLKFRGTPM 180

Query: 181 YMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDC 215
           YMSPESI +G++NT LDIWSL CIL+EM++GKH W DC
Sbjct: 181 YMSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDC 213

[Close]

BLAST of ClCG04G003250 vs. ExPASy TrEMBL
Match: A0A6J1DDK5 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020053 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.6e-52
Identity = 112/226 (49.56%), Postives = 150/226 (66.37%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           +  W RV VLG   +G +FL  P  G + +VA+KV  I  + SL+ E+ IL +F GC EI
Sbjct: 418 LGTWTRVMVLGEGSHGIVFLAKPTMGPIYYVAVKVAFITKSSSLVIEKDILQHFIGCPEI 477

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            QC+G  I  E  SL    YNL LEYAA G+L +LI++ +KLP+ +VKEYL+MILR L  
Sbjct: 478 IQCIGDEITLEESSL---CYNLILEYAAGGTLVNLIKKEKKLPENQVKEYLKMILRGLSC 537

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKK-CDEFEEVVI---TTNAKPRFQ 180
           IH +G+VH D+KP NIL FP D+G        F LSK+   +  EVV+   T ++KP+F+
Sbjct: 538 IHSKGFVHSDLKPTNILAFPRDDGKMKLKIADFELSKRWVHQDHEVVLNTTTASSKPKFK 597

Query: 181 GTPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKSVG 219
           GTP YMSPES++ G I+   DIWSL CIL+EMI+GK VW +C++ G
Sbjct: 598 GTPIYMSPESVVLGSISPPCDIWSLGCILVEMITGKQVWKNCQTSG 640

[Close]

BLAST of ClCG04G003250 vs. ExPASy TrEMBL
Match: A0A6J1DIL5 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111021423 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.1e-51
Identity = 112/224 (50.00%), Postives = 147/224 (65.62%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEI 60
           M  W +VK LG   YG +FL     G +D+VA+KV  +E + SL  E+ IL + +GC EI
Sbjct: 1   MERWTKVKELGRGSYGKVFLAKSTIGPIDYVAVKVAPVERSSSLELEKDILQHLRGCSEI 60

Query: 61  FQCLGSGIGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMILRCLYQ 120
            QC+GS        + S+ Y L LEYA  G+LA+LIR+++KL +++VKEYLRMILR L  
Sbjct: 61  VQCIGS-------EMTSVCYYLILEYAGGGTLAELIRKQKKLTESKVKEYLRMILRGLSC 120

Query: 121 IHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKC----DEFEEVVITTNAKPRFQ 180
           IH +G+VH D+KP NIL FP  NG        FGLSK+     DE  E     +++PRF+
Sbjct: 121 IHRRGFVHADLKPANILAFPRPNGKMKLKIADFGLSKRWVVGQDEAAE-----HSEPRFR 180

Query: 181 GTPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDCKS 217
           GT RYMSPESI+FGKI+  LDIWSL CI IEMI+G  VW+ C++
Sbjct: 181 GTARYMSPESIIFGKISPPLDIWSLGCIFIEMITGSPVWNSCRT 212

[Close]

BLAST of ClCG04G003250 vs. ExPASy TrEMBL
Match: A0A6J1DKV0 (mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia OX=3673 GN=LOC111021457 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.9e-47
Identity = 108/224 (48.21%), Postives = 143/224 (63.84%), Query Frame = 0


Query: 1   MSDWNRVKVLGIDFYGTMFLPVPMSGALDFVAIKVVSIEDAH-----SLMTEETILLNFK 60
           M++W RVK LG   YG +FL  P + +LD+ AIK   +   H     S + E+ IL  F 
Sbjct: 1   MANWIRVKPLGQGSYGEVFLAKPTADSLDYFAIKATQLPLHHCSSFSSPLREKCILECFI 60

Query: 61  GCLEIFQCLGSG-IGFEGDSLDSISYNLALEYAATGSLADLIRRREKLPDTEVKEYLRMI 120
           GC EI QC G   I  EGDS D  S+ L +EYAA GSLADLI++R+KL + EVK+YLRMI
Sbjct: 61  GCPEIVQCYGGEIIIMEGDSFDLASFFLIMEYAAGGSLADLIKKRKKLAENEVKDYLRMI 120

Query: 121 LRCLYQIHEQGYVHGDIKPDNILVFPDDNGNNSC----FGLSKKCDEFEEVVITTNAKPR 180
           LR +  IH++G+VH D+KP NIL FP  +G        FGL+++  E EE       + R
Sbjct: 121 LRGVACIHDKGFVHADLKPGNILAFPQPDGKMKLKITDFGLTRRSCEEEEAYDDYCVERR 180

Query: 181 FQGTPRYMSPESILFGKINTSLDIWSLSCILIEMISGKHVWSDC 215
           F+GTP YMSPES+ +G++   LDIWS+ CI +E++SGK VW  C
Sbjct: 181 FRGTPIYMSPESV-YGQVKAPLDIWSVGCIAVEIVSGKPVWDGC 223

[Close]

BLAST of ClCG04G003250 vs. TAIR 10
Match: AT2G34290.1 (Protein kinase superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 2.8e-35
Identity = 84/185 (45.41%), Postives = 110/185 (59.46%), Query Frame = 0


Query: 35  VVSIEDAHSLMTEETILLNFKGCLEIFQCLGSGIGFEGDSLDSISYNLALEYAATGSLAD 94
           V + +DA SL  E  IL  FKGC  I QC GSG+    +    + Y + +EYAA GSL+D
Sbjct: 33  VKTSDDAKSLYEEFQILSKFKGCSRIVQCYGSGVEQRLNDKGYVEYTIPMEYAAGGSLSD 92

Query: 95  LIRR--REKLPDTEVKEYLRMILRCLYQIHEQGYVHGDIKPDNILVFP----DDNGNNSC 154
            + R   +KLPD  ++++ RM+L  L  IH  GYVH D+KP+NILVFP    D     S 
Sbjct: 93  FMDRFNDKKLPDPMIRKFTRMLLEGLATIHRHGYVHCDLKPENILVFPGSVCDLKLKISD 152

Query: 155 FGLSKKCDEFEEVVITTNAKP--RFQGTPRYMSPESILFGKINTSLDIWSLSCILIEMIS 212
           FGLSK+  +      TT   P   + GTP YMSPESI  G+I   LD+WSL C+++EM +
Sbjct: 153 FGLSKRDGD------TTWWHPLKSYAGTPIYMSPESISHGEIGKGLDLWSLGCVVLEMYT 211

[Close]

BLAST of ClCG04G003250 vs. TAIR 10
Match: AT2G41920.1 (Protein kinase superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.8e-34
Identity = 84/201 (41.79%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 26  GALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEIFQCLGSGIGFEGDSLDSISYNLALE 85
           G   + A+K  S E+A SL  E  IL  FKGC  I QC G+G+    +    + Y +A+E
Sbjct: 31  GETQYAAVKTSSDENAKSLYKEFQILSQFKGCSRIVQCYGNGVKEIFNDKGYVEYKIAME 90

Query: 86  YAATGSLADLIRR--REKLPDTEVKEYLRMILRCLYQIHEQGYVHGDIKPDNILVFPDDN 145
           YA  GSL+D + R    KL D+ ++E+ RM+L  L  IH  GYVH D+KP+NILVFP   
Sbjct: 91  YAFGGSLSDFMDRFKDRKLSDSMIREFTRMLLEGLATIHRHGYVHCDLKPENILVFPSSV 150

Query: 146 GNN-----------SCFGLSKKCDEFEEVVITTNAKPR--FQGTPRYMSPESILFGKINT 205
             N           S FG+SK+  +      T   +PR  + GTP YMSPESI  G+I  
Sbjct: 151 YKNGAWIRSYELKISDFGMSKRDGD------TQWWQPRKPYVGTPIYMSPESISHGEIGK 210

Query: 206 SLDIWSLSCILIEMISGKHVW 212
            LD+WSL C+++EM + K  W
Sbjct: 211 GLDLWSLGCVVLEMYTRKKPW 225

[Close]

BLAST of ClCG04G003250 vs. TAIR 10
Match: AT2G05060.1 (Protein kinase superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 5.2e-34
Identity = 85/211 (40.28%), Postives = 117/211 (55.45%), Query Frame = 0


Query: 8   KVLGIDFYGTMFL---PVPMSGALDFVAIKVVSIEDAHSLMTEETILLNFKGCLEIFQCL 67
           KVLG    G++ L      + G   + A+K  +I  A SL+ E  IL  FKGC  I QC 
Sbjct: 18  KVLGKGSSGSVSLIKYKSRLDGQTLYAAVKTSNIIHADSLLKEFQILSEFKGCSRIVQCY 77

Query: 68  GSGIGFEGDSLDSISYNLALEYAATGSLADLIRRRE--KLPDTEVKEYLRMILRCLYQIH 127
           G+ +    +    + + + +EYA+ GSL   + R +  KLPD  ++ + RMIL  L  IH
Sbjct: 78  GTKVQETINEEGDVEFTIPMEYASGGSLRHFMSRFKDMKLPDALIRRFTRMILEGLAVIH 137

Query: 128 EQGYVHGDIKPDNILVFPDDNGNNSCFGLSKKCDEFEEVVITTNAKPRFQGTPRYMSPES 187
             GYVH D+KP+NILVFP      S FGLSK+  + +  +        F GTP YMSPES
Sbjct: 138 GHGYVHCDLKPENILVFPSFELKISDFGLSKREGDSKWWL----PSHPFAGTPVYMSPES 197

Query: 188 ILFGKINTSLDIWSLSCILIEMISGKHVWSD 214
           I  G+    LD+WSL C+++EM +GK  W D
Sbjct: 198 ISNGETRRGLDLWSLGCVVLEMYTGKRPWWD 224

[Close]

BLAST of ClCG04G003250 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-31
Identity = 77/190 (40.53%), Postives = 106/190 (55.79%), Query Frame = 0


Query: 30  FVAIKVVSIEDAHSLMTEETILLNFKGCLEIFQCLGSGIGFEGDSLDSIS-YNLALEYAA 89
           + A+K    ED +SL  E  IL   +GC  I QC G+    E   +     Y + +EYAA
Sbjct: 35  YAAVKTAECEDYNSLEREIQILSKLEGCRRIVQCYGNYTLEEDFDVGGFRVYKMVMEYAA 94

Query: 90  TGSLADLI--RRREKLPDTEVKEYLRMILRCLYQIHEQGYVHGDIKPDNILVFPDDNGNN 149
            GSL   +   +  KLP+T +K++ RMIL+ L  +H  GYVH D+KPDN+LVFP      
Sbjct: 95  AGSLFSFMDSYKDRKLPETMIKDFTRMILQGLVSVHRLGYVHCDLKPDNLLVFPCRQSYE 154

Query: 150 ---SCFGLSKKCDEFEEVVITTNAKPRFQGTPRYMSPESILFGKINTSLDIWSLSCILIE 209
              S FG S+K  E+ +     +    F GTP YMSPES+  G    +LD+WSL CI++E
Sbjct: 155 LKISDFGSSRKVGEYSD---CWDVDLPFVGTPVYMSPESVRSGVAEKALDLWSLGCIVLE 214

Query: 210 MISGKHVWSD 214
           M +G   WS+
Sbjct: 215 MYTGVIPWSE 221

[Close]

BLAST of ClCG04G003250 vs. TAIR 10
Match: AT5G27790.1 (Protein kinase superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 5.4e-31
Identity = 77/190 (40.53%), Postives = 104/190 (54.74%), Query Frame = 0


Query: 35  VVSIEDAHSLMTEETILLNFKGCLEIFQCLGSGIGFEGDSLDSISYNLALEYAATGSLAD 94
           V + +DA SL  E  IL  FKGC  I QC G+G+    +    + Y + +EYA  GSL +
Sbjct: 52  VKTSDDAKSLYEEFQILSKFKGCPRIVQCYGNGVKQRFNDKGYVEYMIPMEYATGGSLNN 111

Query: 95  LIRR--REKLPDTEVKEYLRMILRCLYQIHEQGYVHGDIKPDNILVFPDDNGNN------ 154
            + R    KLPD  ++++ RM+L  L  IH  GYVH DIKP+NILVFP            
Sbjct: 112 FMDRFNDRKLPDPMIRKFTRMLLEGLATIHRYGYVHYDIKPENILVFPGSVYKEGAWRYS 171

Query: 155 -----SCFGLSKKCDEFEEVVITTNAKPRFQGTPRYMSPESILFGKINTSLDIWSLSCIL 212
                S FGLSK+  + +      +    + GT  YMSPESI  G+I   LD+WSL C++
Sbjct: 172 YKLKISDFGLSKRDGDTK----WWHPLKSYAGTRIYMSPESISHGEIGKGLDLWSLGCVV 231

The following BLAST results are available for this feature:

BLAST of ClCG04G003250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896352.1	3.7e-90	76.58	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_038896344.1	6.3e-90	76.13	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_038896328.1	1.4e-84	74.77	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_038896342.1	5.1e-84	72.07	mitogen-activated protein kinase kinase kinase 3-like [Benincasa hispida]	[more]
XP_038896341.1	2.5e-70	72.25	mitogen-activated protein kinase kinase 1-like [Benincasa hispida]	[more]

BLAST of ClCG04G003250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	1.3e-26	33.33	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
P28829	5.3e-23	31.78	Protein kinase byr2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28...	[more]
Q54R82	2.1e-19	30.19	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
Q8T2I8	3.5e-19	30.00	Serine/threonine-protein kinase sepA OS=Dictyostelium discoideum OX=44689 GN=sep...	[more]
Q40541	1.3e-18	32.13	Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097...	[more]

BLAST of ClCG04G003250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHA9	1.7e-53	50.00	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A6J1D9A6	8.6e-53	51.38	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A6J1DDK5	5.6e-52	49.56	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020053 ...	[more]
A0A6J1DIL5	8.1e-51	50.00	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A6J1DKV0	1.9e-47	48.21	mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia ...	[more]

BLAST of ClCG04G003250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34290.1	2.8e-35	45.41	Protein kinase superfamily protein	[more]
AT2G41920.1	1.8e-34	41.79	Protein kinase superfamily protein	[more]
AT2G05060.1	5.2e-34	40.28	Protein kinase superfamily protein	[more]
AT2G42550.1	1.1e-31	40.53	Protein kinase superfamily protein	[more]
AT5G27790.1	5.4e-31	40.53	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 4..217 e-value: 5.1E-11 score: 23.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 29..212 e-value: 6.3E-30 score: 104.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 4..218 score: 26.407272
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 2..217 e-value: 2.5E-36 score: 127.2
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 1..215
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..215
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 126..138
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG04G003250.1	ClCG04G003250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds
molecular_function	GO:0004672	protein kinase activity