Homology
BLAST of ClCG03G002490 vs. NCBI nr
Match:
XP_023521064.1 (berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 880.6 bits (2274), Expect = 8.6e-252
Identity = 432/531 (81.36%), Postives = 471/531 (88.70%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPS 189
NL +FL CVS DDSN I TPSS +YSSVLNFT+RNLRFSGPN PKP AII PS
Sbjct: 3 NLDDFLRCVSN-----DDSNPILINTPSSSTYSSVLNFTVRNLRFSGPNTPKPCAIINPS 62
Query: 190 QPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVAN-LPFLLIDLANLNAVTVE-DDNTA 249
Q S VQ +IC K HG+HIR+RSGGHDFEGLSYVA+ + F+LID+A LN+V+V+ DDNTA
Sbjct: 63 QASHVQAGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVAFVLIDMAKLNSVSVDTDDNTA 122
Query: 250 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 309
WV+SGASMGELYYKL +TS FGFPGGVQPNVGVGGF SGGGYGLMVRKYGL VDNVVDA
Sbjct: 123 WVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDA 182
Query: 310 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 369
Q+VDANG ++DR SMGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP+IVTSFTAFR W N
Sbjct: 183 QVVDANGRVVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIWDQN 242
Query: 370 STKLVHRWQYVAPEIDHRLFIGIMVTGANS---GGGRIMGVFMYSLFLGRAEELISIMDK 429
ST LVH+WQ VAPEID RLFIG MVT ANS GGGRIMGVF+YSLFLGRAEE+ISIM+
Sbjct: 243 STNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGGRIMGVFLYSLFLGRAEEVISIMEN 302
Query: 430 NFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPIS 489
NFPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFLLDRTPL NGRYKTKSDYAT+PIS
Sbjct: 303 NFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEPIS 362
Query: 490 ETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQ 549
+TAL GIWERFNAKGI+TVQLVLIPYGGKMN+FSETDTPSPHRA YPIKIAYYVTW++P+
Sbjct: 363 QTALHGIWERFNAKGIQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDTPE 422
Query: 550 DDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFG 609
DSRHLEWTRELYDYM PFVSKSPRAAYVNYRDLDIGRNNE+GIPTSYEEASVWGLKYFG
Sbjct: 423 GDSRHLEWTRELYDYMTPFVSKSPRAAYVNYRDLDIGRNNEEGIPTSYEEASVWGLKYFG 482
Query: 610 DNFERLVEVKTKVDPFNLFRHEQSIPPVARGGAQAKMSSCPDVSYNAMQYL 656
DNFERLVEVKTKVDP+NLFRHEQSIPPVA AQ K+SS DV+ + M+YL
Sbjct: 483 DNFERLVEVKTKVDPYNLFRHEQSIPPVA---AQVKISSGNDVACDKMEYL 525
BLAST of ClCG03G002490 vs. NCBI nr
Match:
XP_022964765.1 (berberine bridge enzyme-like 26 [Cucurbita moschata])
HSP 1 Score: 877.1 bits (2265), Expect = 9.5e-251
Identity = 431/533 (80.86%), Postives = 471/533 (88.37%), Query Frame = 0
Query: 127 FCDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAII 186
F NL +FL CVS DDSN I TPSS +YSSVLNFT+RNLRFSGP+ PKP AII
Sbjct: 23 FGGNLDDFLRCVSN-----DDSNPILINTPSSSTYSSVLNFTVRNLRFSGPDTPKPFAII 82
Query: 187 KPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVAN-LPFLLIDLANLNAVTVE-DD 246
PSQ S VQ +IC K HG+HIR+RSGGHDFEGLSYVA+ +PF+LID+A LN+V+V+ DD
Sbjct: 83 NPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVPFVLIDMAKLNSVSVDTDD 142
Query: 247 NTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNV 306
NTAWV+SGASMGELYYKL +TS FGFPGGVQPNVGVGGF SGGGYGLMVRKYGL VDNV
Sbjct: 143 NTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNV 202
Query: 307 VDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSW 366
VDAQ+VDANG L+DR SMGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP+IVTSFTAFR W
Sbjct: 203 VDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIW 262
Query: 367 GPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS--GGGRIMGVFMYSLFLGRAEELISIM 426
NST LVH+WQ VAPEID RLFIG MVT ANS GGGRIMGVF+YSLFLGRAEE+ISIM
Sbjct: 263 DQNSTNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGRIMGVFLYSLFLGRAEEVISIM 322
Query: 427 DKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKP 486
+ NFPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFLLDRTPL NGRYKTKSDYAT+P
Sbjct: 323 ENNFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEP 382
Query: 487 ISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNS 546
IS+TAL GIWERFNAKGI+TVQLVLIPYGGKMN+FSETDTPSPHRA YPIKIAYYVTW++
Sbjct: 383 ISQTALHGIWERFNAKGIQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDT 442
Query: 547 PQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKY 606
+ DSRHLEWTRELYDYM PFVSKSP+AAYVNYRDLDIGRNNE+GIPTSYEEA VWGLKY
Sbjct: 443 LEGDSRHLEWTRELYDYMTPFVSKSPKAAYVNYRDLDIGRNNEEGIPTSYEEAGVWGLKY 502
Query: 607 FGDNFERLVEVKTKVDPFNLFRHEQSIPPVARGGAQAKMSSCPDVSYNAMQYL 656
FGDNFERLVEVKTKVDP+NLFRHEQSIPPVA AQ K+SS DV+ + M+YL
Sbjct: 503 FGDNFERLVEVKTKVDPYNLFRHEQSIPPVA---AQVKISSGNDVACDKMEYL 547
BLAST of ClCG03G002490 vs. NCBI nr
Match:
XP_023525672.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 805.4 bits (2079), Expect = 3.5e-229
Identity = 388/513 (75.63%), Postives = 437/513 (85.19%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPS 189
NL +FLHC+S +S SNST +YT SS SYSSVLNFTIRNLRFSGPN+PKPIAIIKPS
Sbjct: 26 NLEDFLHCISSNS-----SNSTLLYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPS 85
Query: 190 QPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTAW 249
QPSQVQ A+ICC H + IR+RSGGHDFEGLSYVAN+ F++IDL NLN+VTV+ ++NTAW
Sbjct: 86 QPSQVQAAVICCNNHDLRIRVRSGGHDFEGLSYVANVTFVVIDLVNLNSVTVDIENNTAW 145
Query: 250 VQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQ 309
VQSGA+ GE+YYKL ++S+FGFPGGVQP VG+GGF SGGGYG+MVRKYGLG DNVVDA
Sbjct: 146 VQSGATQGEVYYKLGLKSSAFGFPGGVQPTVGIGGFLSGGGYGMMVRKYGLGADNVVDAL 205
Query: 310 LVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNS 369
LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVP VTSF A+RS N+
Sbjct: 206 LVDANGRLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPTVTSFAAYRSLDQNA 265
Query: 370 TKLVHRWQYVAPEIDHRLFIGIMVTGA-------NSGGGRIMGVFMYSLFLGRAEELISI 429
T LVHRWQ+VAP ID RLFIG++V+G GG R G F YSLFLG+AEELI I
Sbjct: 266 TDLVHRWQFVAPVIDERLFIGVIVSGLILILFFFRGGGRRTKGAFFYSLFLGKAEELIPI 325
Query: 430 MDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATK 489
M+K+FPELGLKK DC+E+NWVES AYPASGFVT D+N LLDR PL +GR+KTKSDY T+
Sbjct: 326 MEKSFPELGLKKADCLETNWVESTAYPASGFVTANDLNVLLDRDPLSSGRFKTKSDYITE 385
Query: 490 PISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWN 549
PISE AL+GIWER +A GIETVQLVLIPYGGKMNE +ET+ PSPHR GYPIKI YYVTW
Sbjct: 386 PISEAALDGIWERLDADGIETVQLVLIPYGGKMNEIAETELPSPHRDGYPIKIGYYVTWE 445
Query: 550 SPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLK 609
SP+ D RHLEW+RE+Y+YMAPFVSKSPRAAYVNYRDLDIG NNEDG PTS+EEASVWGLK
Sbjct: 446 SPEADMRHLEWSREVYEYMAPFVSKSPRAAYVNYRDLDIGTNNEDGTPTSFEEASVWGLK 505
Query: 610 YFGDNFERLVEVKTKVDPFNLFRHEQSIPPVAR 635
YFG NFERLV VKT VDP NLFRH+QSIPP+A+
Sbjct: 506 YFGVNFERLVRVKTMVDPCNLFRHQQSIPPMAQ 533
BLAST of ClCG03G002490 vs. NCBI nr
Match:
KAG6583521.1 (Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 785.4 bits (2027), Expect = 3.8e-223
Identity = 398/554 (71.84%), Postives = 444/554 (80.14%), Query Frame = 0
Query: 94 SDHHLPSKTREFFTLHSTIIINGHRGNSHYYYF-FCDNLHNFLH---CVSPHSS-----Q 153
S+ P+ R +H + HR ++ + + LH+++ SP ++
Sbjct: 419 SETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKD 478
Query: 154 LDDSNSTFIYTPSSPSYSSVLN---FTIRNLRFSGPNIPKPIAIIKPSQPSQVQTALICC 213
LD + P+S +SV F I RFSGPN PKP AII PSQ S VQ +IC
Sbjct: 479 LDIGRNNEDGIPTSYDEASVWGYRYFGINFQRFSGPNTPKPFAIINPSQASHVQGGVICA 538
Query: 214 KKHGIHIRIRSGGHDFEGLSYVAN-LPFLLIDLANLNAVTVE-DDNTAWVQSGASMGELY 273
K HG+HIR+RSGGHDFEGLSYVA+ +PF+LID+A LN+V+V+ DDNTAWV+SGASMGELY
Sbjct: 539 KNHGVHIRVRSGGHDFEGLSYVAHGVPFVLIDMAKLNSVSVDTDDNTAWVESGASMGELY 598
Query: 274 YKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQLVDANGDLLDR 333
YKL +TS FGFPGGVQPNVGVGGF SGGGYGLMVRKYGL VDNVVDAQ+VDANG L+DR
Sbjct: 599 YKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDAQVVDANGRLVDR 658
Query: 334 KSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNSTKLVHRWQYVA 393
SMGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP+IVTSFTAFR W NST LVH+WQ VA
Sbjct: 659 DSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVA 718
Query: 394 PEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFPELGLKKEDCVES 453
PEID RLFIG MVT GRAEE+ISIM+ NFPE+GL+ EDCVES
Sbjct: 719 PEIDERLFIGAMVT-------------------GRAEEVISIMENNFPEVGLRMEDCVES 778
Query: 454 NWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISETALEGIWERFNAKG 513
NWVESMAYPASGFVT KDMNFLLDRTPL NGRYKTKSDYAT+PIS+TAL GIWERFNAKG
Sbjct: 779 NWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEPISQTALHGIWERFNAKG 838
Query: 514 IETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDDSRHLEWTRELYDY 573
I+TVQLVLIPYGGKMN+FSETDTPSPHRA YPIKIAYYVTW++P+ DSRHLEWTRELYDY
Sbjct: 839 IQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDTPEGDSRHLEWTRELYDY 898
Query: 574 MAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDP 633
M PFVSKSPRAAYVNYRDLDIGRNNE+GIPTSYEEASVWGLKYFGDNFERLVEVKTKVDP
Sbjct: 899 MTPFVSKSPRAAYVNYRDLDIGRNNEEGIPTSYEEASVWGLKYFGDNFERLVEVKTKVDP 953
BLAST of ClCG03G002490 vs. NCBI nr
Match:
KAG6607170.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 778.9 bits (2010), Expect = 3.5e-221
Identity = 374/484 (77.27%), Postives = 419/484 (86.57%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPS 189
NL +FLHC+S +S SNST +YT SS SYSSVLNFTIRNLRFSGPN+PKPIAIIKPS
Sbjct: 26 NLEDFLHCISSNS-----SNSTLLYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPS 85
Query: 190 QPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTAW 249
QPSQVQ A+ICC H + IR+RSGGHDFEGLSYVAN+ F++IDL NLN+VTV+ ++NTAW
Sbjct: 86 QPSQVQAAVICCNNHDLRIRVRSGGHDFEGLSYVANVTFVVIDLVNLNSVTVDTENNTAW 145
Query: 250 VQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQ 309
VQSGA+ GE+YYKL Q+TS+FGFPGGVQP VG+GGF SGGGYG+MVR+YGLG DNVVDA
Sbjct: 146 VQSGATQGEVYYKLGQKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLGADNVVDAL 205
Query: 310 LVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNS 369
LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVP VTSF A+RS N+
Sbjct: 206 LVDANGRLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPTVTSFAAYRSLDQNA 265
Query: 370 TKLVHRWQYVAPEIDHRLFIGIMVTGANS---GGGRIMGVFMYSLFLGRAEELISIMDKN 429
T LVHRWQ+VAP ID RLFIG++V+ ANS GGGR G F YSLFLG+AEELI IM+K+
Sbjct: 266 TDLVHRWQFVAPVIDERLFIGVIVSAANSMEGGGGRTKGAFFYSLFLGKAEELIPIMEKS 325
Query: 430 FPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISE 489
FPELGLKKEDC+E+NWVES AYPASGFVT D+N LLDR PL +GRYKTKSDY T+PISE
Sbjct: 326 FPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPLSSGRYKTKSDYITEPISE 385
Query: 490 TALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQD 549
AL+GIWER + GIETVQLVLIPYGGKMNE +ET+ PSPHRAGYPIKI YYVTW S +
Sbjct: 386 AALDGIWERLDGDGIETVQLVLIPYGGKMNEIAETELPSPHRAGYPIKIGYYVTWESGEA 445
Query: 550 DSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGD 609
D RHLEW+RE+Y+YMAPFVSKSPRAAYVNYRDLDIG NNEDG PTS+EEASVWGLKYFG
Sbjct: 446 DMRHLEWSREVYEYMAPFVSKSPRAAYVNYRDLDIGTNNEDGTPTSFEEASVWGLKYFGV 504
BLAST of ClCG03G002490 vs. ExPASy Swiss-Prot
Match:
Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)
HSP 1 Score: 484.2 bits (1245), Expect = 2.4e-135
Identity = 242/506 (47.83%), Postives = 339/506 (67.00%), Query Frame = 0
Query: 134 FLHCVSPHSSQLDDSNSTFIY-TPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPS 193
F++CV ++ TF T + +S VL T +NLRF ++PKP I P S
Sbjct: 35 FINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHES 94
Query: 194 QVQTALICCKKHGIHIRIRSGGHDFEGLSYVANL--PFLLIDLANLNAVTVE-DDNTAWV 253
VQ ++IC KK +H+R+RSGGHD+EGLSYV+ + PF+L+DL+ + V + DN+AWV
Sbjct: 95 HVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWV 154
Query: 254 QSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQL 313
QSGA++GELYY++++++ GFP G+ ++G+GG +GG YG M+RKYGLG DNV+DA++
Sbjct: 155 QSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKI 214
Query: 314 VDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN-S 373
VDANG LLDR +MGED FWAIRGG GGSFGI+LAWK+KLVPVP VT FT ++ +
Sbjct: 215 VDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVG 274
Query: 374 TKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFPE 433
K++ +WQ VA ++ LFI ++ A +GG + + +LFLG L+++M K+FPE
Sbjct: 275 NKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPE 334
Query: 434 LGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD-RTPLKNGRYKTKSDYATKPISETA 493
LGL +DC+E +W+ES+AY SGF T N LL ++P +K KSD+ PI E+
Sbjct: 335 LGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESG 394
Query: 494 LEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTW-NSPQDD 553
L+GI+++ + I ++ PYGG M + E+ P PHR G K+ Y +W +S +
Sbjct: 395 LQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRP 454
Query: 554 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDN 613
SRH+ W R+LY YM P+VS +PR AYVNYRDLD+GRN +D + T ++A VWG YF +N
Sbjct: 455 SRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNN 514
Query: 614 FERLVEVKTKVDPFNLFRHEQSIPPV 633
F RL+ +K KVDP N FRHEQSIPP+
Sbjct: 515 FNRLMMIKAKVDPENFFRHEQSIPPM 536
BLAST of ClCG03G002490 vs. ExPASy Swiss-Prot
Match:
Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)
HSP 1 Score: 482.6 bits (1241), Expect = 6.9e-135
Identity = 238/511 (46.58%), Postives = 333/511 (65.17%), Query Frame = 0
Query: 133 NFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPS 192
NFL C S + + +N FIYT Y SVLN TI+NLRF+ PKP+ I+ PS S
Sbjct: 33 NFLKCFSEYIPN-NPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 92
Query: 193 QVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTAWVQS 252
+Q +++C KK G+ IR RSGGHD EGLSY++ +PF ++DL N++ V V+ TAWV++
Sbjct: 93 HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 152
Query: 253 GASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQLVD 312
GA++GE+YY +++ +F FPGG P VGVGG FSGGGYG ++R YGL DN++DA LV+
Sbjct: 153 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 212
Query: 313 ANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNS-TK 372
+G +LDRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+ T F+ ++ + K
Sbjct: 213 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 272
Query: 373 LVHRWQYVAPEIDHRLFIGI-----MVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKN 432
L ++WQ +A + D L + +T + + + S+FLG + L+ +M+K+
Sbjct: 273 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 332
Query: 433 FPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF----LLDRTPLKNGRYKTKSDYATK 492
FPELG+KK DC E +W+++ + SG V NF LLDR+ K + K DY K
Sbjct: 333 FPELGIKKTDCKELSWIDTTIF-YSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKK 392
Query: 493 PISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWN 552
I ETA+ I E+ + + VL PYGG M+E SE+ P PHRAG ++ Y TW
Sbjct: 393 LIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWE 452
Query: 553 SPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLK 612
+D+ +H+ W R +Y++ P+VS++PR AY+NYRDLD+G+ N + P +Y +A +WG K
Sbjct: 453 KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPES-PNNYTQARIWGEK 512
Query: 613 YFGDNFERLVEVKTKVDPFNLFRHEQSIPPV 633
YFG NF RLV+VKTK DP N FR+EQSIPP+
Sbjct: 513 YFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540
BLAST of ClCG03G002490 vs. ExPASy Swiss-Prot
Match:
Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)
HSP 1 Score: 478.8 bits (1231), Expect = 9.9e-134
Identity = 234/511 (45.79%), Postives = 332/511 (64.97%), Query Frame = 0
Query: 133 NFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPS 192
NFL C S H + +N +YT Y S+LN TI+NLRF PKP+ I+ PS S
Sbjct: 33 NFLKCFSKHIPN-NVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 92
Query: 193 QVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTAWVQS 252
+Q ++C KK G+ IR RSGGHD EG+SY++ +PF+++DL N++++ ++ TAWV++
Sbjct: 93 HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 152
Query: 253 GASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQLVD 312
GA++GE+YY ++++ + FPGG P VGVGG FSGGGYG ++R YGL DN++DA LV+
Sbjct: 153 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 212
Query: 313 ANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNS-TK 372
+G +LDRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+ T F+ ++ + K
Sbjct: 213 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 272
Query: 373 LVHRWQYVAPEIDHRL-----FIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKN 432
L ++WQ +A + D L FI +T + + + S+F G + L+ +M+K+
Sbjct: 273 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 332
Query: 433 FPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF----LLDRTPLKNGRYKTKSDYATK 492
FPELG+KK DC E +W+++ + SG V NF LLDR+ K + K DY K
Sbjct: 333 FPELGIKKTDCKEFSWIDTTIF-YSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKK 392
Query: 493 PISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWN 552
PI ETA+ I E+ + + VL PYGG M E SE+ P PHRAG ++ Y +W
Sbjct: 393 PIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE 452
Query: 553 SPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLK 612
+D+ +H+ W R +Y++ P+VS++PR AY+NYRDLD+G+ N P +Y +A +WG K
Sbjct: 453 KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNH-ASPNNYTQARIWGEK 512
Query: 613 YFGDNFERLVEVKTKVDPFNLFRHEQSIPPV 633
YFG NF RLV+VKTKVDP N FR+EQSIPP+
Sbjct: 513 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540
BLAST of ClCG03G002490 vs. ExPASy Swiss-Prot
Match:
Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)
HSP 1 Score: 476.5 bits (1225), Expect = 4.9e-133
Identity = 237/506 (46.84%), Postives = 341/506 (67.39%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSN--STFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIK 189
N +FL C+ S QL+DSN S I+TP+ S+SSVL +I+N RFS P++PKP+ I+
Sbjct: 33 NQSSFLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILT 92
Query: 190 PSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVEDDN-T 249
P QPS VQ+A+ C ++ GIHIR RSGGHD+EGLSYV + PF+++DL NL ++TV+ DN +
Sbjct: 93 PVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRS 152
Query: 250 AWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVD 309
WVQ+GA++GELYY++ ++ + FP GV P VGVGG FSGGGYG ++RK+GL D+V+D
Sbjct: 153 VWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVID 212
Query: 310 AQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGP 369
A++VDA G +L+R+ MGED FWAIRGGGG SF +VL+WK+ L+ VP+ VT F +
Sbjct: 213 ARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQ 272
Query: 370 NSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNF 429
++ K++HRWQ+VA ++ LFI +M+ + ++ L+LG + L+ +++K F
Sbjct: 273 SALKIIHRWQFVADKVSDDLFIRVMLQRYKN----MVRASFPGLYLGSVKNLLKMVNKEF 332
Query: 430 PELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISET 489
PELGL+++DC E +W+ES+ + A + + +N L RT +K KSD+ +P+ +T
Sbjct: 333 PELGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKT 392
Query: 490 ALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDD 549
A+ +W R E QL+ P+GGKM+E ++ +TP PHR G +I Y W +
Sbjct: 393 AISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRGDVKE 452
Query: 550 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTS-YEEASVWGLKYFGD 609
+++ W +YD M+ FV+KSPR AY+N RDLD+G G+ S YEE WG+KYF +
Sbjct: 453 -KYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKN 512
Query: 610 NFERLVEVKTKVDPFNLFRHEQSIPP 632
NFERLV VKT VDP + F EQSIPP
Sbjct: 513 NFERLVRVKTSVDPSDFFCDEQSIPP 525
BLAST of ClCG03G002490 vs. ExPASy Swiss-Prot
Match:
Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)
HSP 1 Score: 476.5 bits (1225), Expect = 4.9e-133
Identity = 230/508 (45.28%), Postives = 333/508 (65.55%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPS 189
N NFL C+ S ++++ +S I+T PSY S+LN +I+N RF PKP++II P
Sbjct: 33 NHENFLKCL---SHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPV 92
Query: 190 QPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVA-NLPFLLIDLANLNAVTVEDDN-TA 249
Q + VQ+ + C + HGIHIR RSGGHD+EGLSY+A + PF++IDL NL ++T++ DN T
Sbjct: 93 QATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTG 152
Query: 250 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 309
WVQSGA++GELYY++ + + S FP G+ P VG+GG F GGGYG ++RKYGL DNV+DA
Sbjct: 153 WVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDA 212
Query: 310 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 369
+VDANG LDR+ MGED FWAIRGGGG SF +VL+WK++L+ VP++VT F ++
Sbjct: 213 HIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKE 272
Query: 370 STKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFP 429
+ ++++WQY+A ++ + LFI M+ G L+LG +L+++M FP
Sbjct: 273 AVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFPG-----LYLGPVSDLLALMKDKFP 332
Query: 430 ELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISETA 489
ELGL+ +C E +W+ES+ + F+ + M L R +K K D+ +PI +TA
Sbjct: 333 ELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTA 392
Query: 490 LEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDDS 549
++ +W RF A +++L P+GGKM+E ++ + P PHR G +I Y W+ +D +
Sbjct: 393 IQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKN 452
Query: 550 -----RHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKY 609
++L W +Y++M P+VSKSPR AYVN+RD+D+G + T YEEA VWG+KY
Sbjct: 453 KTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKY 512
Query: 610 FGDNFERLVEVKTKVDPFNLFRHEQSIP 631
F +NF+RLV VKT VDP + F EQSIP
Sbjct: 513 FKNNFDRLVRVKTNVDPMDFFCDEQSIP 527
BLAST of ClCG03G002490 vs. ExPASy TrEMBL
Match:
A0A6J1HLR7 (berberine bridge enzyme-like 26 OS=Cucurbita moschata OX=3662 GN=LOC111464766 PE=3 SV=1)
HSP 1 Score: 877.1 bits (2265), Expect = 4.6e-251
Identity = 431/533 (80.86%), Postives = 471/533 (88.37%), Query Frame = 0
Query: 127 FCDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAII 186
F NL +FL CVS DDSN I TPSS +YSSVLNFT+RNLRFSGP+ PKP AII
Sbjct: 23 FGGNLDDFLRCVSN-----DDSNPILINTPSSSTYSSVLNFTVRNLRFSGPDTPKPFAII 82
Query: 187 KPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVAN-LPFLLIDLANLNAVTVE-DD 246
PSQ S VQ +IC K HG+HIR+RSGGHDFEGLSYVA+ +PF+LID+A LN+V+V+ DD
Sbjct: 83 NPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVPFVLIDMAKLNSVSVDTDD 142
Query: 247 NTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNV 306
NTAWV+SGASMGELYYKL +TS FGFPGGVQPNVGVGGF SGGGYGLMVRKYGL VDNV
Sbjct: 143 NTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNV 202
Query: 307 VDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSW 366
VDAQ+VDANG L+DR SMGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP+IVTSFTAFR W
Sbjct: 203 VDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIW 262
Query: 367 GPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANS--GGGRIMGVFMYSLFLGRAEELISIM 426
NST LVH+WQ VAPEID RLFIG MVT ANS GGGRIMGVF+YSLFLGRAEE+ISIM
Sbjct: 263 DQNSTNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGRIMGVFLYSLFLGRAEEVISIM 322
Query: 427 DKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKP 486
+ NFPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFLLDRTPL NGRYKTKSDYAT+P
Sbjct: 323 ENNFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEP 382
Query: 487 ISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNS 546
IS+TAL GIWERFNAKGI+TVQLVLIPYGGKMN+FSETDTPSPHRA YPIKIAYYVTW++
Sbjct: 383 ISQTALHGIWERFNAKGIQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDT 442
Query: 547 PQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKY 606
+ DSRHLEWTRELYDYM PFVSKSP+AAYVNYRDLDIGRNNE+GIPTSYEEA VWGLKY
Sbjct: 443 LEGDSRHLEWTRELYDYMTPFVSKSPKAAYVNYRDLDIGRNNEEGIPTSYEEAGVWGLKY 502
Query: 607 FGDNFERLVEVKTKVDPFNLFRHEQSIPPVARGGAQAKMSSCPDVSYNAMQYL 656
FGDNFERLVEVKTKVDP+NLFRHEQSIPPVA AQ K+SS DV+ + M+YL
Sbjct: 503 FGDNFERLVEVKTKVDPYNLFRHEQSIPPVA---AQVKISSGNDVACDKMEYL 547
BLAST of ClCG03G002490 vs. ExPASy TrEMBL
Match:
A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)
HSP 1 Score: 765.4 bits (1975), Expect = 2.0e-217
Identity = 366/527 (69.45%), Postives = 432/527 (81.97%), Query Frame = 0
Query: 129 DNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKP 188
DNL +FLHC+S HSS SNST I+TPSS SY VLNF+IRNLRF+ + PKP+AI+ P
Sbjct: 47 DNLQDFLHCISLHSSN-HHSNSTLIHTPSSSSYPHVLNFSIRNLRFAESHTPKPVAIVTP 106
Query: 189 SQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVEDDN-TA 248
QPS+VQ +ICCK HG+ IR RSGGHDFEG SYV+N+PF+LIDL NLN++T++ DN TA
Sbjct: 107 WQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLIDLINLNSITIDVDNETA 166
Query: 249 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 308
W QSGA++GELYYK+ +++ + FP G ++G+GGFFSGGG G++VRKYGL VDNVVDA
Sbjct: 167 WAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGLGMLVRKYGLAVDNVVDA 226
Query: 309 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 368
LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV VP VTSF +SW N
Sbjct: 227 YLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLN 286
Query: 369 STKLVHRWQYVAPEIDHRLFIGIMVTGANS----GGGRIMGVFMYSLFLGRAEELISIMD 428
+ KL+HRWQYVAP++D LFI VT +NS GGGR+M +SLFLG+A EL+S+M
Sbjct: 287 AAKLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMKASFFSLFLGKAAELVSLMK 346
Query: 429 KNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPI 488
K FPEL LKKEDC+E +WVE+MA+ AS +V+ +++ LLDRTPL GRYK KSDYATKPI
Sbjct: 347 KAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTPLITGRYKMKSDYATKPI 406
Query: 489 SETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSP 548
SETAL GIWERF + +ETVQL+LIP+GGKMNE SET TP+PHRA +PI I YYVTW+ P
Sbjct: 407 SETALNGIWERFKIEELETVQLILIPFGGKMNEISETATPNPHRASHPIHIGYYVTWHRP 466
Query: 549 QDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYF 608
DSRHLEWTREL+DYM PFVS+SPRAAY+NY+DLDIGRNNEDGIPTSY+EASVWG +YF
Sbjct: 467 DADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRNNEDGIPTSYDEASVWGYRYF 526
Query: 609 GDNFERLVEVKTKVDPFNLFRHEQSIPPVA-RGGAQAKMSSCPDVSY 650
G NFERLVEVKTKVDPFN F+HE+SIPP A G QAKM+SCPDVSY
Sbjct: 527 GINFERLVEVKTKVDPFNFFQHEESIPPAAPMGVIQAKMASCPDVSY 572
BLAST of ClCG03G002490 vs. ExPASy TrEMBL
Match:
A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)
HSP 1 Score: 759.6 bits (1960), Expect = 1.1e-215
Identity = 366/527 (69.45%), Postives = 430/527 (81.59%), Query Frame = 0
Query: 126 FFCDNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAI 185
F DNL +FLHC+S HSS SNST I+TPSS SY VLNF+IRNLRF+ + PKP+AI
Sbjct: 39 FGSDNLEDFLHCISLHSSN-RHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAI 98
Query: 186 IKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DD 245
+ P Q SQVQ +ICCK HG+ IR RSGGHDFEG SYV+N+PF+LIDL NLN++T++ DD
Sbjct: 99 VTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDD 158
Query: 246 NTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNV 305
TAW QSGA++GELYYK+ +++ + FP G+ ++G+GGFFSGGG G++VRKYGL VDNV
Sbjct: 159 ETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNV 218
Query: 306 VDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSW 365
VDA LVDANG LLDR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV VP VTSF +SW
Sbjct: 219 VDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSW 278
Query: 366 GPNSTKLVHRWQYVAPEIDHRLFIGIMVTGANSG--GGRIMGVFMYSLFLGRAEELISIM 425
N+ KL+H WQYVA E+D LFI VT +NS GGRIM +SLFLG+A EL+S+M
Sbjct: 279 DLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLGKAAELVSLM 338
Query: 426 DKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKP 485
+K FPEL LKKEDC+E +WVE+MA+ AS +V+ +++ LLDRT L GRYK KSDYATKP
Sbjct: 339 EKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITGRYKMKSDYATKP 398
Query: 486 ISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNS 545
ISETAL GIWERF + +ETVQL+LIP+GGKMNE SETDTP+PHRA +PI I YYVTW+
Sbjct: 399 ISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHR 458
Query: 546 PQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKY 605
P DSRHLEWTREL+DYM PFVS+SPRAAY+NY+DLDIG+NNEDGIPTSY+EASVWG +Y
Sbjct: 459 PDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRY 518
Query: 606 FGDNFERLVEVKTKVDPFNLFRHEQSIPPVA-RGGAQAKMSSCPDVS 649
FG NFERLVEVKTKVDPFN FRHEQSIPP A GG QAKM+SCPD S
Sbjct: 519 FGINFERLVEVKTKVDPFNFFRHEQSIPPAAPMGGIQAKMASCPDES 564
BLAST of ClCG03G002490 vs. ExPASy TrEMBL
Match:
A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)
HSP 1 Score: 759.6 bits (1960), Expect = 1.1e-215
Identity = 372/536 (69.40%), Postives = 433/536 (80.78%), Query Frame = 0
Query: 129 DNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKP 188
DNL +FLHC+S HSS +SNST I+TPSS SY VLNF+IRNLRF+ + PKP+AI+ P
Sbjct: 33 DNLQDFLHCISLHSSN-HNSNSTLIHTPSSSSYLYVLNFSIRNLRFAESDTPKPVAIVTP 92
Query: 189 SQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTA 248
Q S+VQT +ICCK HG+ IR RSGG DFEG SYV+N+PF+LIDL NLN++T++ +D TA
Sbjct: 93 WQASEVQTTVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLIDLTNLNSITIDTEDETA 152
Query: 249 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 308
W QSGA++GELYYK+ +++ + FP G+ N+G+GGFFSGGG+G++VRKYGL VDNVVDA
Sbjct: 153 WAQSGATVGELYYKIGEKSRTLAFPAGLVANLGLGGFFSGGGFGMLVRKYGLAVDNVVDA 212
Query: 309 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 368
QLVDANG LLDR SMGEDLFWAIRGGGGGSFGIVL WKLKLV VP VTSF +SW N
Sbjct: 213 QLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVRVPPTVTSFAVHKSWDLN 272
Query: 369 STKLVHRWQYVAPEIDHRLFIGIMVTGANS--GGGRIMGVFMYSLFLGRAEELISIMDKN 428
+ KL+HRWQYVAPE D LFI V +NS GGGRIM +SLFLG A EL+S+M K
Sbjct: 273 AAKLIHRWQYVAPEADEDLFITAWVIASNSSDGGGRIMKASFFSLFLGEAAELVSLMAKA 332
Query: 429 FPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDR-TPLKNGRYKTKSDYATKPIS 488
FPEL L KEDC+E +WVESMA+ AS FV+ + + LLDR T L NGRYK KSDYAT+PIS
Sbjct: 333 FPELELTKEDCLEMSWVESMAFSASVFVSAESLELLLDRTTQLSNGRYKIKSDYATEPIS 392
Query: 489 ETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQ 548
ETAL GIWERF + +ET+QLVLIP+GGKMNE SETDTP+PHRA +PI I YY TW+ P
Sbjct: 393 ETALNGIWERFKIEELETLQLVLIPFGGKMNEISETDTPNPHRARHPIHIGYYATWHRPD 452
Query: 549 DDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFG 608
DSRHLEWTREL+DYM PFVSKSPRAAY+N +DLDIGRNNEDGIPTSYEEAS+WG +YFG
Sbjct: 453 ADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNEDGIPTSYEEASIWGHQYFG 512
Query: 609 DNFERLVEVKTKVDPFNLFRHEQSIPPVAR-GGAQAKMSSCPDVSY----NAMQYL 656
NFERLV VKTK DPFN FRHEQSIPPVA GG QAKM+SC DVSY +AMQYL
Sbjct: 513 INFERLVAVKTKADPFNFFRHEQSIPPVAPIGGIQAKMASCSDVSYETMPSAMQYL 567
BLAST of ClCG03G002490 vs. ExPASy TrEMBL
Match:
A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)
HSP 1 Score: 755.4 bits (1949), Expect = 2.0e-214
Identity = 367/536 (68.47%), Postives = 435/536 (81.16%), Query Frame = 0
Query: 129 DNLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKP 188
DNL +FLHC+S HSS +SNST I+TPSS SY VLNF+IRNLRF+ + PKP+AI+ P
Sbjct: 33 DNLQDFLHCISLHSSN-HNSNSTLIHTPSSSSYLYVLNFSIRNLRFAESDTPKPVAIVTP 92
Query: 189 SQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVE-DDNTA 248
Q SQVQ +ICCK HG+ IR RSGG DFEG SYV+N+PF+LIDL NLN++T++ +D TA
Sbjct: 93 WQASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLIDLINLNSITIDTEDETA 152
Query: 249 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 308
W QSGA++GELYYK+ Q++ + FP G+ N+G+GGFFSGGG+G+++RKYGL VDNVVDA
Sbjct: 153 WAQSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGGGFGMLLRKYGLAVDNVVDA 212
Query: 309 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 368
QLVDANG LLDR SMGEDLFWAIRGGGGGSFGIVL WKLKLV VP VTSF +SW N
Sbjct: 213 QLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVRVPPTVTSFAVHKSWDLN 272
Query: 369 STKLVHRWQYVAPEIDHRLFIGIMVTGANS--GGGRIMGVFMYSLFLGRAEELISIMDKN 428
+ KL+H+WQYVAPE+D LFI VT +NS GGGRIM ++LFLG A EL+S+M K
Sbjct: 273 AAKLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKASFFALFLGEAAELVSLMAKA 332
Query: 429 FPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDR-TPLKNGRYKTKSDYATKPIS 488
FPEL L +EDC+E +WVESMA+ AS FV+ +++ LLDR T L NGRYK KSDYAT+PIS
Sbjct: 333 FPELELTREDCLEMSWVESMAFSASVFVSAENLELLLDRTTQLSNGRYKIKSDYATEPIS 392
Query: 489 ETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQ 548
ETAL GIWERF + +ET+QL+LIP+GGKMNE SETDTP+PHRA +PI I YY TW+ P
Sbjct: 393 ETALNGIWERFKIEELETLQLILIPFGGKMNEISETDTPNPHRARHPIHIGYYATWHRPD 452
Query: 549 DDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFG 608
DSRHLEWTREL+DYM PFVSKSPRAAY+N +DLDIGRNNEDGIPTSYEEAS+WG +YFG
Sbjct: 453 ADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNEDGIPTSYEEASIWGHQYFG 512
Query: 609 DNFERLVEVKTKVDPFNLFRHEQSIPPVAR-GGAQAKMSSCPDVSY----NAMQYL 656
NFERLVEVKTK DPFN FRHEQSIPPVA G QAKM+SC D+SY +A+QYL
Sbjct: 513 INFERLVEVKTKADPFNFFRHEQSIPPVAPIRGIQAKMASCSDMSYETMPSALQYL 567
BLAST of ClCG03G002490 vs. TAIR 10
Match:
AT5G44400.1 (FAD-binding Berberine family protein )
HSP 1 Score: 484.2 bits (1245), Expect = 1.7e-136
Identity = 242/506 (47.83%), Postives = 339/506 (67.00%), Query Frame = 0
Query: 134 FLHCVSPHSSQLDDSNSTFIY-TPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPS 193
F++CV ++ TF T + +S VL T +NLRF ++PKP I P S
Sbjct: 35 FINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHES 94
Query: 194 QVQTALICCKKHGIHIRIRSGGHDFEGLSYVANL--PFLLIDLANLNAVTVE-DDNTAWV 253
VQ ++IC KK +H+R+RSGGHD+EGLSYV+ + PF+L+DL+ + V + DN+AWV
Sbjct: 95 HVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWV 154
Query: 254 QSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQL 313
QSGA++GELYY++++++ GFP G+ ++G+GG +GG YG M+RKYGLG DNV+DA++
Sbjct: 155 QSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKI 214
Query: 314 VDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN-S 373
VDANG LLDR +MGED FWAIRGG GGSFGI+LAWK+KLVPVP VT FT ++ +
Sbjct: 215 VDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVG 274
Query: 374 TKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFPE 433
K++ +WQ VA ++ LFI ++ A +GG + + +LFLG L+++M K+FPE
Sbjct: 275 NKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPE 334
Query: 434 LGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD-RTPLKNGRYKTKSDYATKPISETA 493
LGL +DC+E +W+ES+AY SGF T N LL ++P +K KSD+ PI E+
Sbjct: 335 LGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESG 394
Query: 494 LEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTW-NSPQDD 553
L+GI+++ + I ++ PYGG M + E+ P PHR G K+ Y +W +S +
Sbjct: 395 LQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRP 454
Query: 554 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDN 613
SRH+ W R+LY YM P+VS +PR AYVNYRDLD+GRN +D + T ++A VWG YF +N
Sbjct: 455 SRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNN 514
Query: 614 FERLVEVKTKVDPFNLFRHEQSIPPV 633
F RL+ +K KVDP N FRHEQSIPP+
Sbjct: 515 FNRLMMIKAKVDPENFFRHEQSIPPM 536
BLAST of ClCG03G002490 vs. TAIR 10
Match:
AT4G20820.1 (FAD-binding Berberine family protein )
HSP 1 Score: 476.5 bits (1225), Expect = 3.5e-134
Identity = 237/506 (46.84%), Postives = 341/506 (67.39%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSN--STFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIK 189
N +FL C+ S QL+DSN S I+TP+ S+SSVL +I+N RFS P++PKP+ I+
Sbjct: 33 NQSSFLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILT 92
Query: 190 PSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANLPFLLIDLANLNAVTVEDDN-T 249
P QPS VQ+A+ C ++ GIHIR RSGGHD+EGLSYV + PF+++DL NL ++TV+ DN +
Sbjct: 93 PVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRS 152
Query: 250 AWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVD 309
WVQ+GA++GELYY++ ++ + FP GV P VGVGG FSGGGYG ++RK+GL D+V+D
Sbjct: 153 VWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVID 212
Query: 310 AQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGP 369
A++VDA G +L+R+ MGED FWAIRGGGG SF +VL+WK+ L+ VP+ VT F +
Sbjct: 213 ARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQ 272
Query: 370 NSTKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNF 429
++ K++HRWQ+VA ++ LFI +M+ + ++ L+LG + L+ +++K F
Sbjct: 273 SALKIIHRWQFVADKVSDDLFIRVMLQRYKN----MVRASFPGLYLGSVKNLLKMVNKEF 332
Query: 430 PELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISET 489
PELGL+++DC E +W+ES+ + A + + +N L RT +K KSD+ +P+ +T
Sbjct: 333 PELGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKT 392
Query: 490 ALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDD 549
A+ +W R E QL+ P+GGKM+E ++ +TP PHR G +I Y W +
Sbjct: 393 AISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRGDVKE 452
Query: 550 SRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTS-YEEASVWGLKYFGD 609
+++ W +YD M+ FV+KSPR AY+N RDLD+G G+ S YEE WG+KYF +
Sbjct: 453 -KYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKN 512
Query: 610 NFERLVEVKTKVDPFNLFRHEQSIPP 632
NFERLV VKT VDP + F EQSIPP
Sbjct: 513 NFERLVRVKTSVDPSDFFCDEQSIPP 525
BLAST of ClCG03G002490 vs. TAIR 10
Match:
AT5G44410.1 (FAD-binding Berberine family protein )
HSP 1 Score: 476.5 bits (1225), Expect = 3.5e-134
Identity = 230/508 (45.28%), Postives = 333/508 (65.55%), Query Frame = 0
Query: 130 NLHNFLHCVSPHSSQLDDSNSTFIYTPSSPSYSSVLNFTIRNLRFSGPNIPKPIAIIKPS 189
N NFL C+ S ++++ +S I+T PSY S+LN +I+N RF PKP++II P
Sbjct: 33 NHENFLKCL---SHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPV 92
Query: 190 QPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVA-NLPFLLIDLANLNAVTVEDDN-TA 249
Q + VQ+ + C + HGIHIR RSGGHD+EGLSY+A + PF++IDL NL ++T++ DN T
Sbjct: 93 QATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTG 152
Query: 250 WVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDA 309
WVQSGA++GELYY++ + + S FP G+ P VG+GG F GGGYG ++RKYGL DNV+DA
Sbjct: 153 WVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDA 212
Query: 310 QLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPN 369
+VDANG LDR+ MGED FWAIRGGGG SF +VL+WK++L+ VP++VT F ++
Sbjct: 213 HIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKE 272
Query: 370 STKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMYSLFLGRAEELISIMDKNFP 429
+ ++++WQY+A ++ + LFI M+ G L+LG +L+++M FP
Sbjct: 273 AVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFPG-----LYLGPVSDLLALMKDKFP 332
Query: 430 ELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISETA 489
ELGL+ +C E +W+ES+ + F+ + M L R +K K D+ +PI +TA
Sbjct: 333 ELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTA 392
Query: 490 LEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQDDS 549
++ +W RF A +++L P+GGKM+E ++ + P PHR G +I Y W+ +D +
Sbjct: 393 IQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKN 452
Query: 550 -----RHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKY 609
++L W +Y++M P+VSKSPR AYVN+RD+D+G + T YEEA VWG+KY
Sbjct: 453 KTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKY 512
Query: 610 FGDNFERLVEVKTKVDPFNLFRHEQSIP 631
F +NF+RLV VKT VDP + F EQSIP
Sbjct: 513 FKNNFDRLVRVKTNVDPMDFFCDEQSIP 527
BLAST of ClCG03G002490 vs. TAIR 10
Match:
AT5G44380.1 (FAD-binding Berberine family protein )
HSP 1 Score: 464.5 bits (1194), Expect = 1.4e-130
Identity = 238/526 (45.25%), Postives = 343/526 (65.21%), Query Frame = 0
Query: 123 YYYFFC--------DNLHNFLHCVSPHSSQLDDSNSTFIYTPSS--PSYSSVLNFTIRNL 182
Y+ F+C F++CV ++ T ++TP+ ++ VL T +NL
Sbjct: 19 YFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKT-LFTPAKNVSLFNQVLESTAQNL 78
Query: 183 RFSGPNIPKPIAIIKPSQPSQVQTALICCKKHGIHIRIRSGGHDFEGLSYVANL--PFLL 242
+F ++PKP I +P SQVQ ++IC KK GIH R+RSGGHDFE LSYV+ + PF+L
Sbjct: 79 QFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFIL 138
Query: 243 IDLANLNAVTVE-DDNTAWVQSGASMGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGG 302
+DL+ L + V+ + N+AWVQ GA++GELYY++++++ GFP G+ +VG+GG+ +GGG
Sbjct: 139 LDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGG 198
Query: 303 YGLMVRKYGLGVDNVVDAQLVDANGDLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 362
YG ++RKYGL DNV+D ++VDANG LLDR +MGEDLFWAIRGGGG SFGIVLAWK+KLV
Sbjct: 199 YGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLV 258
Query: 363 PVPAIVTSFTAFRSWGPNS-TKLVHRWQYVAPEIDHRLFIGIMVTGANSGGGRIMGVFMY 422
PVP VT FT ++ ++ K + +WQ ++ +I + I +++ A + G + + +
Sbjct: 259 PVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDGNKTVTMTYL 318
Query: 423 SLFLGRAEELISIMDKNFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLLD-RTPL 482
FLG L+ +M+K FPELGL ++DC E +W+E+ + GF T + LL ++PL
Sbjct: 319 GQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALF-HGGFPTGSPIEILLQLKSPL 378
Query: 483 KNGRYKTKSDYATKPISETALEGIWERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHR 542
+K SD+ +PI L+GI++R +G T L PYGG M++ E+ P PHR
Sbjct: 379 GKDYFKATSDFVKEPIPVIGLKGIFKRL-IEG-NTTFLNWTPYGGMMSKIPESAIPFPHR 438
Query: 543 AGYPIKIAYYVTW-NSPQDDSRHLEWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNED 602
G KI YY W + + SR + W +E+Y+YMAP+VS +PR AYVNYRDLD G+ N++
Sbjct: 439 NGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQ-NKN 498
Query: 603 GIPTSYEEASVWGLKYFGDNFERLVEVKTKVDPFNLFRHEQSIPPV 633
++ EA +WG KYF NF+RLV++KTKVDP N FRHEQSIPP+
Sbjct: 499 NAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539
BLAST of ClCG03G002490 vs. TAIR 10
Match:
AT2G34790.1 (FAD-binding Berberine family protein )
HSP 1 Score: 464.2 bits (1193), Expect = 1.8e-130
Identity = 228/500 (45.60%), Postives = 333/500 (66.60%), Query Frame = 0
Query: 145 LDDSNSTFIYTPS--SPS-----YSSVLNFTIRNLRFSGPNIPKPIAIIKPSQPSQVQTA 204
+D+S+ +F T S SP + L T +NLR+ P+ PKP+ I +P + VQ A
Sbjct: 38 VDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAA 97
Query: 205 LICCKKHGIHIRIRSGGHDFEGLSYVA--NLPFLLIDLANLNAVTVE-DDNTAWVQSGAS 264
++C KK +H+R+RSGGHD+EGLS+VA PF+++DL+ L V V+ D N+AW +GA+
Sbjct: 98 VVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGAT 157
Query: 265 MGELYYKLSQQTSSFGFPGGVQPNVGVGGFFSGGGYGLMVRKYGLGVDNVVDAQLVDANG 324
+GE+YY++ +++ + GFP G+ ++G+GG GG YG M+RK+GLG DNV+DA++VDANG
Sbjct: 158 IGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANG 217
Query: 325 DLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTSFTAFRSWGPNSTKLVHR 384
+LDR +MGED+FWAIRGGGGGSFG++LAWK+KLVPVPA VT FT ++ + TK++++
Sbjct: 218 QILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYK 277
Query: 385 WQYVAPEIDHRLFIGIMVTGAN---SGGGRIMGVFMYSLFLGRAEELISIMDKNFPELGL 444
W+ +A ++D LFI ++++ A+ G R + + + FLG + L+ +M K+FPELGL
Sbjct: 278 WEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGL 337
Query: 445 KKEDCVESNWVESMAYPASGFVTTKDMNFLLDRTPLKNGRYKTKSDYATKPISETALEGI 504
K+DC E +W++S+ Y A GF + LL L +K KSD+ +PI LEG+
Sbjct: 338 TKKDCTEMSWIKSVMYIA-GFPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGL 397
Query: 505 WERFNAKGIETVQLVLIPYGGKMNEFSETDTPSPHRAGYPIKIAYYVTWNSPQ-DDSRHL 564
WERF + ++ + PYGG M+ SE++ P PHR G KI + TW + + RH+
Sbjct: 398 WERFLEE--DSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHM 457
Query: 565 EWTRELYDYMAPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFGDNFERL 624
+W RE+Y YM +VSK+PR AYVNYRDLD+G N + +A WG KY+ NFERL
Sbjct: 458 KWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE------TDAREWGAKYYKGNFERL 517
Query: 625 VEVKTKVDPFNLFRHEQSIP 631
V++K + DP N FRHEQS+P
Sbjct: 518 VKIKGEFDPDNFFRHEQSVP 528
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_023521064.1 | 8.6e-252 | 81.36 | berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022964765.1 | 9.5e-251 | 80.86 | berberine bridge enzyme-like 26 [Cucurbita moschata] | [more] |
| XP_023525672.1 | 3.5e-229 | 75.63 | berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo] | [more] |
| KAG6583521.1 | 3.8e-223 | 71.84 | Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG6607170.1 | 3.5e-221 | 77.27 | Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9FKU8 | 2.4e-135 | 47.83 | Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... | [more] |
| Q33DQ2 | 6.9e-135 | 46.58 | Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1 | [more] |
| Q8GTB6 | 9.9e-134 | 45.79 | Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... | [more] |
| Q9SVG5 | 4.9e-133 | 46.84 | Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... | [more] |
| Q9FI25 | 4.9e-133 | 45.28 | Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1HLR7 | 4.6e-251 | 80.86 | berberine bridge enzyme-like 26 OS=Cucurbita moschata OX=3662 GN=LOC111464766 PE... | [more] |
| A0A6J1HP64 | 2.0e-217 | 69.45 | berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE... | [more] |
| A0A6J1HYZ7 | 1.1e-215 | 69.45 | berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3... | [more] |
| A0A6J1EVN7 | 1.1e-215 | 69.40 | berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE... | [more] |
| A0A6J1I038 | 2.0e-214 | 68.47 | berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3... | [more] |
