ClCG02G019460 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG02G019460
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionAsparagine synthetase [glutamine-hydrolyzing]
LocationCG_Chr02: 34177905 .. 34178087 (+)
RNA-Seq ExpressionClCG02G019460
SyntenyClCG02G019460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGGAATTCTTGCGGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTTCCTAACCTTCATTCTTTATAGTGTTGTTGATCAAATTGAATCGGAAATAATTAAGTATCGTGCAATTCAAGATTATGAATAG

mRNA sequence

ATGTGTGGAATTCTTGCGGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTTCCTAACCTTCATTCTTTATAGTGTTGTTGATCAAATTGAATCGGAAATAATTAAGTATCGTGCAATTCAAGATTATGAATAG

Coding sequence (CDS)

ATGTGTGGAATTCTTGCGGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTTCCTAACCTTCATTCTTTATAGTGTTGTTGATCAAATTGAATCGGAAATAATTAAGTATCGTGCAATTCAAGATTATGAATAG

Protein sequence

MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTFILYSVVDQIESEIIKYRAIQDYE
Homology
[Close]
BLAST of ClCG02G019460 vs. NCBI nr
Match: CDP09210.1 (unnamed protein product [Coffea canephora])

HSP 1 Score: 65.9 bits (159), Expect = 1.4e-07
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTF 38
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F++FF  +
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQFFILFFFFY 37

[Close]
BLAST of ClCG02G019460 vs. NCBI nr
Match: MBA0615314.1 (hypothetical protein [Gossypium davidsonii])

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQV  + FL
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVIFLSFL 35

[Close]
BLAST of ClCG02G019460 vs. NCBI nr
Match: KAA3458584.1 (asparagine synthetase [glutamine-hydrolyzing] 1 [Gossypium australe])

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-07
Identity = 30/35 (85.71%), Postives = 32/35 (91.43%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQ+ + F L
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQIHIFFLL 35

[Close]
BLAST of ClCG02G019460 vs. NCBI nr
Match: KAF6170425.1 (hypothetical protein GIB67_014355 [Kingdonia uniflora])

HSP 1 Score: 62.4 bits (150), Expect = 1.5e-06
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIF 34
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F +F
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQCFSLF 33

[Close]
BLAST of ClCG02G019460 vs. NCBI nr
Match: KAG7035357.1 (Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 61.2 bits (147), Expect = 3.4e-06
Identity = 34/42 (80.95%), Postives = 35/42 (83.33%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQV---FLIFFLTFIL 40
          MCGILAVLGCSDDSQAKRVRVLELSRRQV     IF L F+L
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVLNLLCIFPLVFVL 42

[Close]
BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P49078 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P49093 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=AS2 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P49091 (Asparagine synthetase [glutamine-hydrolyzing] OS=Brassica oleracea OX=3712 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: O24338 (Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca OX=61864 GN=AND1 PE=2 SV=3)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A068UL30 (Asparagine synthetase [glutamine-hydrolyzing] OS=Coffea canephora OX=49390 GN=GSCOC_T00028440001 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.8e-08
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTF 38
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F++FF  +
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQFFILFFFFY 37

[Close]
BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A7J8RNA2 (Glutamine amidotransferase type-2 domain-containing protein (Fragment) OS=Gossypium davidsonii OX=34287 GN=Godav_015468 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQV  + FL
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVIFLSFL 35

[Close]
BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A5B6UQC0 (Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=asns PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-07
Identity = 30/35 (85.71%), Postives = 32/35 (91.43%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQ+ + F L
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQIHIFFLL 35

[Close]
BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A7J7NT90 (Asparagine synthetase [glutamine-hydrolyzing] OS=Kingdonia uniflora OX=39325 GN=GIB67_014355 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.5e-07
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIF 34
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F +F
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQCFSLF 33

[Close]
BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A5B6UE31 (Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=asns PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-06
Identity = 31/39 (79.49%), Postives = 32/39 (82.05%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTFIL 40
          MCGILAVLGCSDDSQAKRVRVLELSRRQ   +F    IL
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQFIFLFSFLLIL 39

[Close]
BLAST of ClCG02G019460 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 57.0 bits (136), Expect = 6.0e-09
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. TAIR 10
Match: AT3G47340.3 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 57.0 bits (136), Expect = 6.0e-09
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. TAIR 10
Match: AT3G47340.2 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 57.0 bits (136), Expect = 6.0e-09
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]
BLAST of ClCG02G019460 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-06
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGC D+SQAKR R++ELSRR
Sbjct: 1  MCGILAVLGCVDNSQAKRSRIIELSRR 27

[Close]
BLAST of ClCG02G019460 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-06
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 0 

Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGC D+SQAKR R++ELSRR
Sbjct: 1  MCGILAVLGCIDNSQAKRSRIIELSRR 27

The following BLAST results are available for this feature:
BLAST of ClCG02G019460 vs. NCBI nr
Match NameE-valueIdentityDescription
CDP09210.11.4e-0783.78unnamed protein product [Coffea canephora][more]
MBA0615314.19.1e-0788.57hypothetical protein [Gossypium davidsonii][more]
KAA3458584.19.1e-0785.71asparagine synthetase [glutamine-hydrolyzing] 1 [Gossypium australe][more]
KAF6170425.11.5e-0690.91hypothetical protein GIB67_014355 [Kingdonia uniflora][more]
KAG7035357.13.4e-0680.95Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma... [more]
BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P490788.5e-08100.00Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 ... [more]
P490938.5e-08100.00Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=A... [more]
P317528.5e-08100.00Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P... [more]
P490918.5e-08100.00Asparagine synthetase [glutamine-hydrolyzing] OS=Brassica oleracea OX=3712 PE=2 ... [more]
O243388.5e-08100.00Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca OX=61864... [more]
BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A068UL306.8e-0883.78Asparagine synthetase [glutamine-hydrolyzing] OS=Coffea canephora OX=49390 GN=GS... [more]
A0A7J8RNA24.4e-0788.57Glutamine amidotransferase type-2 domain-containing protein (Fragment) OS=Gossyp... [more]
A0A5B6UQC04.4e-0785.71Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=... [more]
A0A7J7NT907.5e-0790.91Asparagine synthetase [glutamine-hydrolyzing] OS=Kingdonia uniflora OX=39325 GN=... [more]
A0A5B6UE311.7e-0679.49Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=... [more]
BLAST of ClCG02G019460 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G47340.16.0e-09100.00glutamine-dependent asparagine synthase 1 [more]
AT3G47340.36.0e-09100.00glutamine-dependent asparagine synthase 1 [more]
AT3G47340.26.0e-09100.00glutamine-dependent asparagine synthase 1 [more]
AT5G10240.11.3e-0681.48asparagine synthetase 3 [more]
AT5G65010.11.3e-0681.48asparagine synthetase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSRPIRSR001589-1PIRSR001589-1coord: 1..27
e-value: 5.9E-5
score: 19.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG02G019460.2ClCG02G019460.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0006541 glutamine metabolic process
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
molecular_function GO:0005524 ATP binding