ClCG02G019460 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG02G019460
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Asparagine synthetase [glutamine-hydrolyzing]
Location	CG_Chr02: 34177905 .. 34178087 (+)
RNA-Seq Expression	ClCG02G019460
Synteny	ClCG02G019460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGGAATTCTTGCGGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTTCCTAACCTTCATTCTTTATAGTGTTGTTGATCAAATTGAATCGGAAATAATTAAGTATCGTGCAATTCAAGATTATGAATAG

mRNA sequence

ATGTGTGGAATTCTTGCGGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTTCCTAACCTTCATTCTTTATAGTGTTGTTGATCAAATTGAATCGGAAATAATTAAGTATCGTGCAATTCAAGATTATGAATAG

Coding sequence (CDS)

ATGTGTGGAATTCTTGCGGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTTCCTAACCTTCATTCTTTATAGTGTTGTTGATCAAATTGAATCGGAAATAATTAAGTATCGTGCAATTCAAGATTATGAATAG

Protein sequence

MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTFILYSVVDQIESEIIKYRAIQDYE

Homology

[Close]

BLAST of ClCG02G019460 vs. NCBI nr
Match: CDP09210.1 (unnamed protein product [Coffea canephora])

HSP 1 Score: 65.9 bits (159), Expect = 1.4e-07
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTF 38
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F++FF  +
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQFFILFFFFY 37

[Close]

BLAST of ClCG02G019460 vs. NCBI nr
Match: MBA0615314.1 (hypothetical protein [Gossypium davidsonii])

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQV  + FL
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVIFLSFL 35

[Close]

BLAST of ClCG02G019460 vs. NCBI nr
Match: KAA3458584.1 (asparagine synthetase [glutamine-hydrolyzing] 1 [Gossypium australe])

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-07
Identity = 30/35 (85.71%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQ+ + F L
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQIHIFFLL 35

[Close]

BLAST of ClCG02G019460 vs. NCBI nr
Match: KAF6170425.1 (hypothetical protein GIB67_014355 [Kingdonia uniflora])

HSP 1 Score: 62.4 bits (150), Expect = 1.5e-06
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIF 34
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F +F
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQCFSLF 33

[Close]

BLAST of ClCG02G019460 vs. NCBI nr
Match: KAG7035357.1 (Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 61.2 bits (147), Expect = 3.4e-06
Identity = 34/42 (80.95%), Postives = 35/42 (83.33%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQV---FLIFFLTFIL 40
          MCGILAVLGCSDDSQAKRVRVLELSRRQV     IF L F+L
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVLNLLCIFPLVFVL 42

[Close]

BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P49078 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P49093 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=AS2 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: P49091 (Asparagine synthetase [glutamine-hydrolyzing] OS=Brassica oleracea OX=3712 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot
Match: O24338 (Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca OX=61864 GN=AND1 PE=2 SV=3)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A068UL30 (Asparagine synthetase [glutamine-hydrolyzing] OS=Coffea canephora OX=49390 GN=GSCOC_T00028440001 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.8e-08
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTF 38
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F++FF  +
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQFFILFFFFY 37

[Close]

BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A7J8RNA2 (Glutamine amidotransferase type-2 domain-containing protein (Fragment) OS=Gossypium davidsonii OX=34287 GN=Godav_015468 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQV  + FL
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVIFLSFL 35

[Close]

BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A5B6UQC0 (Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=asns PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-07
Identity = 30/35 (85.71%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFL 36
          MCGILAVLGCSDDSQAKRVRVLELSRRQ+ + F L
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQIHIFFLL 35

[Close]

BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A7J7NT90 (Asparagine synthetase [glutamine-hydrolyzing] OS=Kingdonia uniflora OX=39325 GN=GIB67_014355 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.5e-07
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIF 34
          MCGILAVLGCSDDSQAKRVRVLELSRRQ F +F
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQCFSLF 33

[Close]

BLAST of ClCG02G019460 vs. ExPASy TrEMBL
Match: A0A5B6UE31 (Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=asns PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-06
Identity = 31/39 (79.49%), Postives = 32/39 (82.05%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQVFLIFFLTFIL 40
          MCGILAVLGCSDDSQAKRVRVLELSRRQ   +F    IL
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRRQFIFLFSFLLIL 39

[Close]

BLAST of ClCG02G019460 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 57.0 bits (136), Expect = 6.0e-09
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. TAIR 10
Match: AT3G47340.3 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 57.0 bits (136), Expect = 6.0e-09
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. TAIR 10
Match: AT3G47340.2 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 57.0 bits (136), Expect = 6.0e-09
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGCSDDSQAKRVRVLELSRR
Sbjct: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 27

[Close]

BLAST of ClCG02G019460 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-06
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGC D+SQAKR R++ELSRR
Sbjct: 1  MCGILAVLGCVDNSQAKRSRIIELSRR 27

[Close]

BLAST of ClCG02G019460 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-06
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 0


Query: 1  MCGILAVLGCSDDSQAKRVRVLELSRR 28
          MCGILAVLGC D+SQAKR R++ELSRR
Sbjct: 1  MCGILAVLGCIDNSQAKRSRIIELSRR 27

The following BLAST results are available for this feature:

BLAST of ClCG02G019460 vs. NCBI nr


Match Name	E-value	Identity	Description
CDP09210.1	1.4e-07	83.78	unnamed protein product [Coffea canephora]	[more]
MBA0615314.1	9.1e-07	88.57	hypothetical protein [Gossypium davidsonii]	[more]
KAA3458584.1	9.1e-07	85.71	asparagine synthetase [glutamine-hydrolyzing] 1 [Gossypium australe]	[more]
KAF6170425.1	1.5e-06	90.91	hypothetical protein GIB67_014355 [Kingdonia uniflora]	[more]
KAG7035357.1	3.4e-06	80.95	Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma...	[more]

BLAST of ClCG02G019460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49078	8.5e-08	100.00	Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 ...	[more]
P49093	8.5e-08	100.00	Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=A...	[more]
P31752	8.5e-08	100.00	Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P...	[more]
P49091	8.5e-08	100.00	Asparagine synthetase [glutamine-hydrolyzing] OS=Brassica oleracea OX=3712 PE=2 ...	[more]
O24338	8.5e-08	100.00	Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca OX=61864...	[more]

BLAST of ClCG02G019460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A068UL30	6.8e-08	83.78	Asparagine synthetase [glutamine-hydrolyzing] OS=Coffea canephora OX=49390 GN=GS...	[more]
A0A7J8RNA2	4.4e-07	88.57	Glutamine amidotransferase type-2 domain-containing protein (Fragment) OS=Gossyp...	[more]
A0A5B6UQC0	4.4e-07	85.71	Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=...	[more]
A0A7J7NT90	7.5e-07	90.91	Asparagine synthetase [glutamine-hydrolyzing] OS=Kingdonia uniflora OX=39325 GN=...	[more]
A0A5B6UE31	1.7e-06	79.49	Asparagine synthetase [glutamine-hydrolyzing] OS=Gossypium australe OX=47621 GN=...	[more]

BLAST of ClCG02G019460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47340.1	6.0e-09	100.00	glutamine-dependent asparagine synthase 1	[more]
AT3G47340.3	6.0e-09	100.00	glutamine-dependent asparagine synthase 1	[more]
AT3G47340.2	6.0e-09	100.00	glutamine-dependent asparagine synthase 1	[more]
AT5G10240.1	1.3e-06	81.48	asparagine synthetase 3	[more]
AT5G65010.1	1.3e-06	81.48	asparagine synthetase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PIRSR	PIRSR001589-1	PIRSR001589-1	coord: 1..27 e-value: 5.9E-5 score: 19.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG02G019460.2	ClCG02G019460.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006529	asparagine biosynthetic process
biological_process	GO:0006541	glutamine metabolic process
molecular_function	GO:0004066	asparagine synthase (glutamine-hydrolyzing) activity
molecular_function	GO:0005524	ATP binding