ClCG02G004250 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG02G004250
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Glutamyl-tRNA(Gln) amidotransferase subunit A
Location	CG_Chr02: 4338495 .. 4340677 (-)
RNA-Seq Expression	ClCG02G004250
Synteny	ClCG02G004250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTACTCTTCACCACTTAGTTATGTAGCCTTCTCATTGCTTCTGATTGCTGCAGCGGTTCGAGGATTGCCAATCAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCAAGCAAAACCAGCTCACTTCGAGGGGACTTGTTGAGTTCTACATTGGAGAAATTCGCAGACTCAACCCGGTTCTCCATGGGGTCATAGAAATCAACCCAGACGCATGGCTGCAAGCTTACAAGGCTGACAGAGAACGTGAGGCTAAGAAGCCTGGATCGCTTCATGGGCTGCACGGAATTCCAGTATTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCGTTCGCATTGCTTGGTTCTATTGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAGGGCTGGAGCTATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCCCTCACTGCTCCAGCTGGTTTGAGCGCAAGAGGTAGTCAGGGAAAGGTTTGTTACTTGGAATTGGCTCCAAAGACAATTTCCCCACATATTTAGGGGTATTTGGGCCCCCAACTTGAAGTTGGTGGAGTGGACTATTATAGGCCACTTCATGTTTGGGGCTCCAAATATACTAGTGGTGTTTACAATTATTTATAACCTACAATTAACAACAATAATACTATTTGAAATCTCTATTTGTTATAGTATTTACTATTTACTCCCCAAACTAAAATAGTCAGCACTCTAAACACAGACTATTATAACTCAATGACTATAATAACCAACTCAATGCCCCAAACGCCCCTTCAATATTTTTTAAGTACAACTAAAAACCATTTGATTTCTACAATTCTTTTCAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACGGACGGTTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTTGTCGGCATCAAACCAACGGTTGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGTGAGCAATCTTGCAGTTTGCACCCATATCAGCAAAACACTATACAAAGATTATAAGAACTAAAACTATGTATCCTTCACATGTGAAAACAGGCCAATTGGCAGGACAGTGACAGATGCTGTAATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAAAACAGCCTCCAAGTACATTCCATATGTTGGCTATCAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAAAGATTAGGAATTGTGAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAGCCATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGTAAGAAAACCAACATCCCTACCTATCCATACAAATGCACTAATCTTTCCATCATATTCATTGAAACAAACAATTCAATTTGAACATACATAACCAATCTACACAAGGAATCTAACATTGTTGAAATTGAAGGCAAGGTGGTGCTATTTTGATAGACAATCTAGAGATAGAAAACATAGATATCATCTTAAACGTGACTGGAAGTGGAGAATCAGTGGCATTGCTTGCTGAATTCAAACAATCTTTAAATGGGTACTTGAAAGAGCTCGTGGTTTCCCCTGTTCGAAGTTTGGCCGACATAATCGCCTTCAATAACGCAAACCCAGATCAGGTAACACAATATTCAATCCATTTTTCATCGCACAGTGATCGAATTCATTCCCAATCGATTCTGAATCATGGGTGTGCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTGGCGGCCGAAGCCACAAATGGGATCGGCGACGAGCAGACGGCGGCTCTGTTGAACTTAGCGAAGCTGACGGAGGATGGGTTTGAGAAATTGGTGAAGGAGAAGCGGTTGGATGCGGTGGTGACGCCAGGTTCCGGCATAGCTACAGTGCTTGCAATTGGGGGTTTACCGGGAATCAATGTTCCGGCGGGATACGATGGCGGAGGAGTTCCATTTGGGATTAACTTTGGGGGATTGAAGGGTTCAGAGGGGAAGCTGATTGAGGTTGCTTATGGGTTTGAGCAAGCCACTATGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

mRNA sequence

ATGGCCTACTCTTCACCACTTAGTTATGTAGCCTTCTCATTGCTTCTGATTGCTGCAGCGGTTCGAGGATTGCCAATCAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCAAGCAAAACCAGCTCACTTCGAGGGGACTTGTTGAGTTCTACATTGGAGAAATTCGCAGACTCAACCCGGTTCTCCATGGGGTCATAGAAATCAACCCAGACGCATGGCTGCAAGCTTACAAGGCTGACAGAGAACGTGAGGCTAAGAAGCCTGGATCGCTTCATGGGCTGCACGGAATTCCAGTATTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCGTTCGCATTGCTTGGTTCTATTGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAGGGCTGGAGCTATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCCCTCACTGCTCCAGCTGGTTTGAGCGCAAGAGGTAGTCAGGGAAAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACGGACGGTTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTTGTCGGCATCAAACCAACGGTTGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATTGGCAGGACAGTGACAGATGCTGTAATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAAAACAGCCTCCAAGTACATTCCATATGTTGGCTATCAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAAAGATTAGGAATTGTGAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAGCCATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGCAAGGTGGTGCTATTTTGATAGACAATCTAGAGATAGAAAACATAGATATCATCTTAAACGTGACTGGAAGTGGAGAATCAGTGGCATTGCTTGCTGAATTCAAACAATCTTTAAATGGGTACTTGAAAGAGCTCGTGGTTTCCCCTGTTCGAAGTTTGGCCGACATAATCGCCTTCAATAACGCAAACCCAGATCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTGGCGGCCGAAGCCACAAATGGGATCGGCGACGAGCAGACGGCGGCTCTGTTGAACTTAGCGAAGCTGACGGAGGATGGGTTTGAGAAATTGGTGAAGGAGAAGCGGTTGGATGCGGTGGTGACGCCAGGTTCCGGCATAGCTACAGTGCTTGCAATTGGGGGTTTACCGGGAATCAATGTTCCGGCGGGATACGATGGCGGAGGAGTTCCATTTGGGATTAACTTTGGGGGATTGAAGGGTTCAGAGGGGAAGCTGATTGAGGTTGCTTATGGGTTTGAGCAAGCCACTATGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

Coding sequence (CDS)

ATGGCCTACTCTTCACCACTTAGTTATGTAGCCTTCTCATTGCTTCTGATTGCTGCAGCGGTTCGAGGATTGCCAATCAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCAAGCAAAACCAGCTCACTTCGAGGGGACTTGTTGAGTTCTACATTGGAGAAATTCGCAGACTCAACCCGGTTCTCCATGGGGTCATAGAAATCAACCCAGACGCATGGCTGCAAGCTTACAAGGCTGACAGAGAACGTGAGGCTAAGAAGCCTGGATCGCTTCATGGGCTGCACGGAATTCCAGTATTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCGTTCGCATTGCTTGGTTCTATTGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAGGGCTGGAGCTATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCCCTCACTGCTCCAGCTGGTTTGAGCGCAAGAGGTAGTCAGGGAAAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACGGACGGTTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTTGTCGGCATCAAACCAACGGTTGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATTGGCAGGACAGTGACAGATGCTGTAATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAAAACAGCCTCCAAGTACATTCCATATGTTGGCTATCAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAAAGATTAGGAATTGTGAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAGCCATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGCAAGGTGGTGCTATTTTGATAGACAATCTAGAGATAGAAAACATAGATATCATCTTAAACGTGACTGGAAGTGGAGAATCAGTGGCATTGCTTGCTGAATTCAAACAATCTTTAAATGGGTACTTGAAAGAGCTCGTGGTTTCCCCTGTTCGAAGTTTGGCCGACATAATCGCCTTCAATAACGCAAACCCAGATCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTGGCGGCCGAAGCCACAAATGGGATCGGCGACGAGCAGACGGCGGCTCTGTTGAACTTAGCGAAGCTGACGGAGGATGGGTTTGAGAAATTGGTGAAGGAGAAGCGGTTGGATGCGGTGGTGACGCCAGGTTCCGGCATAGCTACAGTGCTTGCAATTGGGGGTTTACCGGGAATCAATGTTCCGGCGGGATACGATGGCGGAGGAGTTCCATTTGGGATTAACTTTGGGGGATTGAAGGGTTCAGAGGGGAAGCTGATTGAGGTTGCTTATGGGTTTGAGCAAGCCACTATGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

Protein sequence

MAYSSPLSYVAFSLLLIAAAVRGLPIREATVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGESVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGIGDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP

Homology

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BLAST of ClCG02G004250 vs. NCBI nr
Match: XP_038887424.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 904.0 bits (2335), Expect = 5.6e-259
Identity = 475/518 (91.70%), Postives = 487/518 (94.02%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLI----------AAAVRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MAYSSPLS VAFSLLLI          +A VRGL IREATV DLQLAFKQNQLTSR LVE
Sbjct: 1   MAYSSPLSSVAFSLLLILVAFCLTAPHSATVRGLSIREATVQDLQLAFKQNQLTSRRLVE 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEI RLNPV+HGVIEINPDA LQAYKADRER AKKPGSL GLHGIPVLLKDNIGTKD
Sbjct: 61  FYIGEIHRLNPVVHGVIEINPDALLQAYKADRERAAKKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVP DAG+VKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPHDAGIVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGP+ISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPAISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+T SKYIP  GY+QFLNP G
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTTSKYIPNGGYKQFLNPIG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDSAIT+AFEDHFNTLKQGGAILIDNLEIENIDIILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSAITEAFEDHFNTLKQGGAILIDNLEIENIDIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           +VALLAEFKQSLN YLKELV SPVRSLADIIAFNNANPDQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AVALLAEFKQSLNEYLKELVASPVRSLADIIAFNNANPDQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD QTAALLNLAKLTEDGFEK+VK+KRLDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQTAALLNLAKLTEDGFEKVVKKKRLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT+IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. NCBI nr
Match: KAA0049736.1 (putative amidase isoform X2 [Cucumis melo var. makuwa] >TYK12141.1 putative amidase isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 899.0 bits (2322), Expect = 1.8e-257
Identity = 471/518 (90.93%), Postives = 483/518 (93.24%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLIAAA----------VRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MA SSPLS VAFSLLLI  A          VRGL IREATVHDLQLAFKQNQLTSR LV 
Sbjct: 1   MANSSPLSSVAFSLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPV+HGVIEINPDA +QAYKADREREA KPGSL GLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASLSEWADFRSLTAPAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+TASKYIPY GY+QFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEI NI+IILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           + ALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNAN DQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD Q AALLNL KLTEDGFEKLVKE +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. NCBI nr
Match: XP_016900445.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 899.0 bits (2322), Expect = 1.8e-257
Identity = 470/518 (90.73%), Postives = 483/518 (93.24%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLIAAA----------VRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MA SSPLS VAFS+LLI  A          VRGL IREATVHDLQLAFKQNQLTSR LV 
Sbjct: 1   MANSSPLSSVAFSMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPV+HGVIEINPDA +QAYKADREREA KPGSL GLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASLSEWADFRSLTAPAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+TASKYIPY GY+QFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEI NIDIILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           + ALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNAN DQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD Q AALLNL KLTEDGFEKLVK+ +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. NCBI nr
Match: XP_031745278.1 (probable amidase At4g34880 [Cucumis sativus] >KGN43234.1 hypothetical protein Csa_020255 [Cucumis sativus])

HSP 1 Score: 897.9 bits (2319), Expect = 4.0e-257
Identity = 472/518 (91.12%), Postives = 483/518 (93.24%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLIAAA----------VRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MA SSP S VAFSLLLI  A          VRGL IREATVHDLQLAFKQNQLTSR LV 
Sbjct: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPV+HGVIEINPDA LQAYKADREREA KPGSL GLHGIPVLLKDNIGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVPRDAGVVKRLR+AGAIILGKASLSEWADFRSL APAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+TASKYIPY GY+QFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAFEDHFNTLKQGGAILIDNLEI +IDIILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           + ALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNAN DQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD Q AA+LNL KLTEDGFEKLVKE +LDAVVTPG+GIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT+IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. NCBI nr
Match: XP_022953021.1 (putative amidase C869.01 [Cucurbita moschata])

HSP 1 Score: 878.6 bits (2269), Expect = 2.5e-251
Identity = 463/510 (90.78%), Postives = 480/510 (94.12%), Query Frame = 0


Query: 1   MAYSS-PLSYVAFSLLL-IAAAVRGLPIREATVHDLQLAFKQNQLTSRGLVEFYIGEIRR 60
           MAYSS  L  VAF L +  +AAVRG  IREATV DLQLAFK+N+LTSRGLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGS 120
           LNPV+HGVIEINPDA LQAYKADREREAKKPGSL GLHGIPVLLKD+IGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQGKNPYVLS 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSL APAGLSARG QGKNPYVLS
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
           ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYND AT+T+SKYIPY GY+QFLN NGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGESVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFN LKQGGAIL+DNLEIENIDIILNVT SGE+VALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGIGDEQTAAL 420
           KQSLN YLKELV SPVRSLADIIAF+NANPDQELLDVFGQEIFLAAEATNGIGD Q AAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFG 480
           LNLAKLT+DGFEK+V+E+RLDAVVTPG  IATVLAIGG PGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

Query: 481 GLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           GLKGSE  LIEVAY FEQAT+IRKPPSFKP
Sbjct: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 510

[Close]

BLAST of ClCG02G004250 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.3e-169
Identity = 310/481 (64.45%), Postives = 382/481 (79.42%), Query Frame = 0


Query: 26  IREATVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADRERE 85
           I+EAT+ D+++AF + +LTS+ LVE Y+  I +LNP+LH VIE NPDA +QA  ADRER+
Sbjct: 40  IQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERD 99

Query: 86  AKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILG 145
            K    L  LHG+PVLLKD+I TKDKLNTTAGSFALLGS+V RDAGVVKRLR +GA+ILG
Sbjct: 100 LKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILG 159

Query: 146 KASLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTET 205
           KASLSEWA FRS + P G SARG QGKNPYVLSA+P GSSSG +ISV AN+ AVS+GTET
Sbjct: 160 KASLSEWAHFRSFSIPDGWSARGLQGKNPYVLSANPSGSSSGSAISVTANLVAVSLGTET 219

Query: 206 DGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDY 265
           DGSIL PAS NSVVGIKP+VGLTSRAGV+P+S RQD+IGPI RTV+DAV +LD IVG+D 
Sbjct: 220 DGSILSPASQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDP 279

Query: 266 NDAATKTASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQ 325
            D ATKTAS++IP  GY+QFL  +GLKGKRLGIV             +   + H  TL++
Sbjct: 280 LDEATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKH----------SSLLDHHIKTLRR 339

Query: 326 GGAILIDNLEIENIDIILNVTGSGESVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNN 385
            GAI+I+NL I NI++I+  T SGE +ALLAEFK SLN YLKELV SPVRSLAD+IA+N 
Sbjct: 340 EGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNE 399

Query: 386 ANPDQELLDVFGQEIFLAAEATNGIGDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPG 445
              +QE +  +GQE+FL AEAT+G+G+++  AL  + +L+ +G EKL++E +LDA+VT G
Sbjct: 400 EFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLG 459

Query: 446 SGIATVLAIGGLPGINVPAGYDGGGVPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPS 505
           S +++VLAIGG PGINVPAGYD GGVP+GI+FGGL+ SE KLIE+A+ FEQAT+IRKPP 
Sbjct: 460 SDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPPK 510

Query: 506 F 507
           F
Sbjct: 520 F 510

[Close]

BLAST of ClCG02G004250 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 7.8e-78
Identity = 196/515 (38.06%), Postives = 277/515 (53.79%), Query Frame = 0


Query: 22  RGLPIREATVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKAD 81
           + + + +AT+  LQ   +   LTS  +V  Y+    ++NP ++G++++NPD    A + D
Sbjct: 62  QNITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELD 121

Query: 82  REREAKKPGSLHG-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAG 141
            ER     G + G LHGIP ++KDN  TKDK++TTAGS+ALLGSIVPRDA VVK+LR AG
Sbjct: 122 DER---ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 181

Query: 142 AIILGKASLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVS 201
           A++ G A+LSEWAD RS     G SARG Q + P+ L+ +P GSSSG +ISVA+N+ A +
Sbjct: 182 AVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFA 241

Query: 202 IGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTI 261
           +GTETDGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAV V  ++
Sbjct: 242 LGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSM 301

Query: 262 VGFDYNDAATKTASKYIPYVG-YQQFL-NPNGLKGKRLGIVRNPFFSFFNDSAITQAFED 321
            G D ND  T   +   P  G Y +FL N   L+G R G+     +       I +  E 
Sbjct: 302 WGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLE- 361

Query: 322 HFNTLKQGGAILIDNLEIENIDII--------LNVTGSGESVALLAEFKQSLNGYLKELV 381
               +++ GAI+ +N    N+D+I        L      E   +  +F  ++  YL E+ 
Sbjct: 362 VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVK 421

Query: 382 VSPVRSLADIIAFNNANPDQE-----LLDVF--GQEIFLAAEATNGIGDEQTAALLNLAK 441
            + + SL DI+ +NN     E     ++  F  GQ+ FLA+    G+ +E     +   +
Sbjct: 422 NTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVR 481

Query: 442 LT--EDGFE---KLVKEKRLDAVVTPG----SGIATV---LAIGGLPGINVPAGYDGGGV 501
            T  ++G +        K  D+ +  G    SG +      A  G P I +P G    G 
Sbjct: 482 RTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 541

Query: 502 PFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSF 507
           PFG+        E +LI+     E     +  P F
Sbjct: 542 PFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKPKF 572

[Close]

BLAST of ClCG02G004250 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 4.7e-67
Identity = 174/488 (35.66%), Postives = 253/488 (51.84%), Query Frame = 0


Query: 34  LQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLH 93
           LQ  ++Q  +    +V+ Y+  I  +N  +  V EINPDA   A + D ER   K G L 
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNER---KMGKLR 153

Query: 94  G-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEW 153
           G LHG+P+++K+NI T DK+++TAGS+A+ G+    DA V  +LR AG +I+GK+  S+W
Sbjct: 154 GPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 154 ADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCP 213
           A+FRSL +  G SA G Q    Y+ +  P GSSSG  ++    +A  ++GTET GSI+ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 214 ASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKT 273
           A  +++VG+KPTVGLTSR  V+P+S RQDT+GP+ R+V DA  +L  I G D ND  T +
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-S 333

Query: 274 ASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILID 333
           A  +     Y +  + N LKGKR+G+ RN    F +   +   F      +K+ GAI+++
Sbjct: 334 AIPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVE 393

Query: 334 NLEIENIDIILNVTGSGESVALLAEFKQSLNGYLKELVVSP--VRSLADIIAFNNANPDQ 393
           N +  +           +   L A+   +L  + K+L V+P  +  L  +  F   +  +
Sbjct: 394 NTDFTSFAEFAQSPIPDD--ILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHHRLE 453

Query: 394 EL--LDVFGQEIFLAAEATNGIGDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGI 453
           E    D    +I L     N           N+    E G    ++  +LDA V P    
Sbjct: 454 EYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLS 513

Query: 454 ATVLAIGGLPGINVPAGY---------------DGGGVPFGINFGGLKGSEGKLIEVAYG 502
             + A+ G P I VP G                 G G+P GI F G   SE KLI +AY 
Sbjct: 514 PYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYA 573

[Close]

BLAST of ClCG02G004250 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.9e-47
Identity = 158/500 (31.60%), Postives = 244/500 (48.80%), Query Frame = 0


Query: 30  TVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKP 89
           T+H+L+   K+ ++++  + + Y+  I+ + P +  ++ I  D  LQ  KA    E  K 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQ--KAKEADEKIKK 66

Query: 90  GSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASL 149
           G    L GIPV++KDNI T + + TT  S  L   I P +A VV++L   G IILGK++L
Sbjct: 67  GEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNL 126

Query: 150 SEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSI 209
            E+A   S    A  +      KNP+ LS  P GSS G + ++AA+ AA ++G++T GSI
Sbjct: 127 DEFAMGSSTENSAFKTT-----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSI 186

Query: 210 LCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAA 269
             PAS   VVG+KPT GL SR G++  +   D IGP  + VTD  IVL+TI+G D  D+ 
Sbjct: 187 RQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDST 246

Query: 270 TKTASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAI 329
           +    K      Y  +L  + +KG R+G+ +  FF    +  + +  ++    L+  GA 
Sbjct: 247 SLKIDK----PDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVLQDLGAE 306

Query: 330 LIDNLEIENIDIIL---NVTGSGESVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNA 389
           +ID + I  ++  L    +  S E+ + LA +     G++ E          D+I     
Sbjct: 307 IID-ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAE-------KYEDLIDMYMV 366

Query: 390 NPDQELLDVFGQEIFLAAEA-TNGIGDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPG 449
              +       + I L   A ++G  D      L +  L ++ FEK    ++ D ++ P 
Sbjct: 367 TRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVIIGPT 426

Query: 450 S-------------GIATVLA--------IGGLPGINVPAGYDGGGVPFGINFGGLKGSE 505
           S              +A  LA        I GLPGI++P G    G+P G+   G    E
Sbjct: 427 SPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVGLQIIGRHFDE 481

[Close]

BLAST of ClCG02G004250 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 9.3e-47
Identity = 160/504 (31.75%), Postives = 247/504 (49.01%), Query Frame = 0


Query: 30  TVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKP 89
           T+H+L+   K+ ++++  + + Y+  I+ + P +  +I I  D  LQ  +A    E  K 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQ--RAKEADEKIKN 66

Query: 90  GSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASL 149
           G    L GIPV++KDNI T + + TT  S  L   I P +A VV++L   G IILGK++L
Sbjct: 67  GEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNL 126

Query: 150 SEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSI 209
            E+A   S    A  +      KNP+ LS  P GSS G + ++AA+ AA ++G++T GSI
Sbjct: 127 DEFAMGSSTENSAFKTT-----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSI 186

Query: 210 LCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAA 269
             PAS   VVG+KPT GL SR G++  +   D IGP  + VTD  IVL+TI+G D  D+ 
Sbjct: 187 RQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDST 246

Query: 270 TKTASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAI 329
           +    K      Y  +L  + +KG R+G+ +  FF    +  + +  ++    L+  GA 
Sbjct: 247 SLKIDK----PDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVLQDLGAE 306

Query: 330 LIDNLEIENIDIIL---NVTGSGESVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNA 389
           +ID + I  ++  L    +  S E+ + LA +     G+    +      L D+     +
Sbjct: 307 IID-ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGH----IAGKYEDLIDMYMVTRS 366

Query: 390 NPDQELLDVFGQE----IFLAAEA-TNGIGDEQTAALLNLAKLTEDGFEKLVKEKRLDAV 449
                  + FG+E    I L   A ++G  D      L +  L ++ FEK    ++ D +
Sbjct: 367 -------EGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVI 426

Query: 450 VTPGS-------------GIATVLA--------IGGLPGINVPAGYDGGGVPFGINFGGL 505
           + P S              +A  LA        I GLPGI++P G    G+P G+   G 
Sbjct: 427 IGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVGLQIIGR 481

[Close]

BLAST of ClCG02G004250 vs. ExPASy TrEMBL
Match: A0A5A7U6F5 (Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00780 PE=4 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.8e-258
Identity = 471/518 (90.93%), Postives = 483/518 (93.24%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLIAAA----------VRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MA SSPLS VAFSLLLI  A          VRGL IREATVHDLQLAFKQNQLTSR LV 
Sbjct: 1   MANSSPLSSVAFSLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPV+HGVIEINPDA +QAYKADREREA KPGSL GLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASLSEWADFRSLTAPAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+TASKYIPY GY+QFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEI NI+IILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           + ALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNAN DQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD Q AALLNL KLTEDGFEKLVKE +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. ExPASy TrEMBL
Match: A0A1S4DXK2 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.8e-258
Identity = 470/518 (90.73%), Postives = 483/518 (93.24%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLIAAA----------VRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MA SSPLS VAFS+LLI  A          VRGL IREATVHDLQLAFKQNQLTSR LV 
Sbjct: 1   MANSSPLSSVAFSMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPV+HGVIEINPDA +QAYKADREREA KPGSL GLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASLSEWADFRSLTAPAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+TASKYIPY GY+QFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEI NIDIILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           + ALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNAN DQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD Q AALLNL KLTEDGFEKLVK+ +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. ExPASy TrEMBL
Match: A0A0A0K2I0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=4 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 2.0e-257
Identity = 472/518 (91.12%), Postives = 483/518 (93.24%), Query Frame = 0


Query: 1   MAYSSPLSYVAFSLLLIAAA----------VRGLPIREATVHDLQLAFKQNQLTSRGLVE 60
           MA SSP S VAFSLLLI  A          VRGL IREATVHDLQLAFKQNQLTSR LV 
Sbjct: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPV+HGVIEINPDA LQAYKADREREA KPGSL GLHGIPVLLKDNIGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQ 180
           KLNTTAGSFALLGSIVPRDAGVVKRLR+AGAIILGKASLSEWADFRSL APAGLSARG Q
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAAT+TASKYIPY GY+QFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAFEDHFNTLKQGGAILIDNLEI +IDIILNVT SGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360

Query: 361 SVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGI 420
           + ALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNAN DQELL+VFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GD Q AA+LNL KLTEDGFEKLVKE +LDAVVTPG+GIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           VPFGINFGGLKGSE KLIEVAYGFEQAT+IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518

[Close]

BLAST of ClCG02G004250 vs. ExPASy TrEMBL
Match: A0A6J1GLV2 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.2e-251
Identity = 463/510 (90.78%), Postives = 480/510 (94.12%), Query Frame = 0


Query: 1   MAYSS-PLSYVAFSLLL-IAAAVRGLPIREATVHDLQLAFKQNQLTSRGLVEFYIGEIRR 60
           MAYSS  L  VAF L +  +AAVRG  IREATV DLQLAFK+N+LTSRGLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGS 120
           LNPV+HGVIEINPDA LQAYKADREREAKKPGSL GLHGIPVLLKD+IGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQGKNPYVLS 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSL APAGLSARG QGKNPYVLS
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
           ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYND AT+T+SKYIPY GY+QFLN NGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGESVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFN LKQGGAIL+DNLEIENIDIILNVT SGE+VALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNGYLKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGIGDEQTAAL 420
           KQSLN YLKELV SPVRSLADIIAF+NANPDQELLDVFGQEIFLAAEATNGIGD Q AAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFG 480
           LNLAKLT+DGFEK+V+E+RLDAVVTPG  IATVLAIGG PGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

Query: 481 GLKGSEGKLIEVAYGFEQATMIRKPPSFKP 509
           GLKGSE  LIEVAY FEQAT+IRKPPSFKP
Sbjct: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 510

[Close]

BLAST of ClCG02G004250 vs. ExPASy TrEMBL
Match: A0A6J1HUS8 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.0e-250
Identity = 456/503 (90.66%), Postives = 474/503 (94.23%), Query Frame = 0


Query: 7   LSYVAFSLLL-IAAAVRGLPIREATVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHG 66
           L  VAF L +  +AAVRG  IREATV DL LAFK+N+LTSRGLVEFYIGEIRRLNPV+HG
Sbjct: 24  LIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIRRLNPVVHG 83

Query: 67  VIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSI 126
           VIEINPDA LQAYKADREREAKKPGSL GLHGIPVLLKD+IGTKDKLNTTAGSFALLGSI
Sbjct: 84  VIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSI 143

Query: 127 VPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSS 186
           VPRDAGVVKRLRRAGAIILGKASLSEWADFRSL APAGLSARG QGKNPYVLSASPCGSS
Sbjct: 144 VPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSS 203

Query: 187 SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGP 246
           SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGP
Sbjct: 204 SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGP 263

Query: 247 IGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFS 306
           IGRTVTDAVIVLDTIVGFDYNDAAT+T+SKYIPY GY+QFLN NGLKGKRLGIVRNPFFS
Sbjct: 264 IGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNPFFS 323

Query: 307 FFNDSAITQAFEDHFNTLKQGGAILIDNLEIENIDIILNVTGSGESVALLAEFKQSLNGY 366
           FFNDSAITQAFEDHFN LKQGGA+L+DNLEI NIDIILNVT SGE+ ALLAEFKQSLN Y
Sbjct: 324 FFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAEFKQSLNEY 383

Query: 367 LKELVVSPVRSLADIIAFNNANPDQELLDVFGQEIFLAAEATNGIGDEQTAALLNLAKLT 426
           LKELV SPVRSLADIIAF+NANPDQELLDVFGQEIFLAAEATNGIGD Q AALLNLAKLT
Sbjct: 384 LKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLT 443

Query: 427 EDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFGGLKGSEG 486
           +DGFEK+V+E+RLDAVVTPG  IATVLAIGG PGINVPAGYDGGGVPFGINFGGLKGSE 
Sbjct: 444 KDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKGSEP 503

Query: 487 KLIEVAYGFEQATMIRKPPSFKP 509
           KLIEVAY FEQAT+IRKPPSFKP
Sbjct: 504 KLIEVAYAFEQATLIRKPPSFKP 526

[Close]

BLAST of ClCG02G004250 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 508.1 bits (1307), Expect = 8.4e-144
Identity = 273/481 (56.76%), Postives = 341/481 (70.89%), Query Frame = 0


Query: 26  IREATVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADRERE 85
           I+EAT+ D+++AF + +LTS+ LVE Y+  I +LNP+LH VIE NPDA +QA  ADRER+
Sbjct: 40  IQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERD 99

Query: 86  AKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILG 145
            K    L  LHG+PVLLKD+I TKDKLNTTAGSFALLGS+V RDAGVVKRLR +GA+ILG
Sbjct: 100 LKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILG 159

Query: 146 KASLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTET 205
           KASLSEWA FRS + P G SA                                       
Sbjct: 160 KASLSEWAHFRSFSIPDGWSA--------------------------------------- 219

Query: 206 DGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDY 265
                   S NSVVGIKP+VGLTSRAGV+P+S RQD+IGPI RTV+DAV +LD IVG+D 
Sbjct: 220 -------PSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDP 279

Query: 266 NDAATKTASKYIPYVGYQQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQ 325
            D ATKTAS++IP  GY+QFL  +GLKGKRLGIV             +   + H  TL++
Sbjct: 280 LDEATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKH----------SSLLDHHIKTLRR 339

Query: 326 GGAILIDNLEIENIDIILNVTGSGESVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNN 385
            GAI+I+NL I NI++I+  T SGE +ALLAEFK SLN YLKELV SPVRSLAD+IA+N 
Sbjct: 340 EGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNE 399

Query: 386 ANPDQELLDVFGQEIFLAAEATNGIGDEQTAALLNLAKLTEDGFEKLVKEKRLDAVVTPG 445
              +QE +  +GQE+FL AEAT+G+G+++  AL  + +L+ +G EKL++E +LDA+VT G
Sbjct: 400 EFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLG 459

Query: 446 SGIATVLAIGGLPGINVPAGYDGGGVPFGINFGGLKGSEGKLIEVAYGFEQATMIRKPPS 505
           S +++VLAIGG PGINVPAGYD GGVP+GI+FGGL+ SE KLIE+A+ FEQAT+IRKPP 
Sbjct: 460 SDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPPK 464

Query: 506 F 507
           F
Sbjct: 520 F 464

[Close]

BLAST of ClCG02G004250 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 122.9 bits (307), Expect = 7.6e-28
Identity = 139/492 (28.25%), Postives = 222/492 (45.12%), Query Frame = 0


Query: 42  QLTSRGLVEFYIGEIRRLNPVLHGVIEINPDAWLQAYKADREREAKKPGSLHGLHGIPVL 101
           + T+  + + Y+  IR   P L   + ++ +    A + D  +   K   L  L G+ + 
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID--QRIAKGEELGPLAGVLIG 116

Query: 102 LKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLTAP 161
           +KDNI T+  + +TA S  L     P DA  VK+++  G I++GK ++ E+    S T  
Sbjct: 117 VKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFG-MGSTTEA 176

Query: 162 AGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGI 221
           +          NP+ LS  P GSS G + +VAA    VS+G++T GS+  PASF  VVG+
Sbjct: 177 SAFQVTA----NPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGL 236

Query: 222 KPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATKTASKYIPYVG 281
           KPT G  SR G++  +   D IG  G TV DA ++L  I G+D  D+   T+SK      
Sbjct: 237 KPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDS---TSSKQDVPEF 296

Query: 282 YQQFLN-----PNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEI 341
             QFL+        L G ++GI+R        DS +  A ++  + L+  G IL + + +
Sbjct: 297 QSQFLSVDHFESKPLNGVKVGIIRETLEDGV-DSGVRSATQEAASHLEALGCILTE-VSL 356

Query: 342 ENIDIIL---NVTGSGESVALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANPDQELL 401
            +  + L    V  S ES + L+ +     G   +++   +  L +         + ++ 
Sbjct: 357 PSFSLGLPAYYVIASSESSSNLSRYDGVRYG--NQVMAEELNKLYECSRGEGFGGEVKMR 416

Query: 402 DVFGQEIFLAA----------EATNGIGDEQTAALLN---LAKLTEDGFEKLVKEKRLDA 461
            + G     A           +    I  +  AAL     L           + EK+ D 
Sbjct: 417 ILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDP 476

Query: 462 VVTPGSGIATV-LAIGGLPGINVPAG-YDGG--GVPFGINFGGLKGSEGKLIEVAYGFEQ 509
           +      I TV + + GLP + +P G  +GG  G+P G+   G    E KL++V + FEQ
Sbjct: 477 LAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQ 530

[Close]

BLAST of ClCG02G004250 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.7e-25
Identity = 80/252 (31.75%), Postives = 131/252 (51.98%), Query Frame = 0


Query: 30  TVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPD-AWLQAYKADREREAKK 89
           +V +L    K  Q+TS+ LV  Y+ +++R N VL  V+    + A+ QA +AD   +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLS 263

Query: 90  PGSLHG-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 149
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 150 SLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDG 209
                A +  +         G + +NP+ +     GSS+GP+ S +A +   +IG+ET G
Sbjct: 324 VTGSMA-YDDIWF-------GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAG 383

Query: 210 SILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYND 269
           S+  PA+   +  ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D
Sbjct: 384 SMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 443

Query: 270 AATKTASKYIPY 280
            +++  +   P+
Sbjct: 444 LSSREIAFEDPF 443

[Close]

BLAST of ClCG02G004250 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 106.7 bits (265), Expect = 5.6e-23
Identity = 79/252 (31.35%), Postives = 128/252 (50.79%), Query Frame = 0


Query: 30  TVHDLQLAFKQNQLTSRGLVEFYIGEIRRLNPVLHGVIEINPD-AWLQAYKADREREAKK 89
           +V +L    K  Q+TS+ LV  Y+ +++R N VL  V+    + A+ QA +AD   +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLS 263

Query: 90  PGSLHG-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 149
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 150 SLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDG 209
                A +  +         G + +NP+ +     GSS+GP+       A+ S G+ET G
Sbjct: 324 VTGSMA-YDDIWF-------GGRTRNPWNIEEFSTGSSAGPA-------ASTSAGSETAG 383

Query: 210 SILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYND 269
           S+  PA+   +  ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D
Sbjct: 384 SMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 436

Query: 270 AATKTASKYIPY 280
            +++  +   P+
Sbjct: 444 LSSREIAFEDPF 436

[Close]

BLAST of ClCG02G004250 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 85.1 bits (209), Expect = 1.8e-16
Identity = 112/446 (25.11%), Postives = 191/446 (42.83%), Query Frame = 0


Query: 76  QAYKADREREAKKPGSLHGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKR 135
           QA  + R  E   P S+  L GI V +KD+I           ++      V +D+ VV +
Sbjct: 180 QAEASTRRFEQGNPISV--LDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSK 239

Query: 136 LRRAGAIILGKASLSEWADFRSLTAPAGLSARGSQGKNPYVLSASPCGSSSGPSISVAAN 195
           LR  GAI+LGKA++ E           G ++     +NP+       GSSSG +  VAA 
Sbjct: 240 LRSCGAILLGKANMHELG-----MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAG 299

Query: 196 IAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVI 255
           + + ++GT+  GS+  P++   + G+K T G T   G +      + IGP+  ++ DA +
Sbjct: 300 LCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFL 359

Query: 256 VLDTIVGFDYNDAATKTASKYIPYVGYQQFLNPNGLKGKRLGIVR-NPFFSFFND---SA 315
           V   I+G   + +A +   K  P   + + L+ NG     +G +R   +  +FND   S 
Sbjct: 360 VYAAILG---SSSADRYNLKPSPPC-FPKLLSHNG--SNAIGSLRLGKYTKWFNDVSSSD 419

Query: 316 ITQAFEDHFNTLKQGGA-----ILIDNLEIENIDIILNVTGSGESVALLAEFKQSLNGYL 375
           I+   ED    L          I++  LE      ++++ GS    +L    +   N  L
Sbjct: 420 ISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHVISI-GSPTLSSLTPYCEAGKNSKL 479

Query: 376 K-----ELVVSPVRSLADIIAFN--NANPDQELLDVFGQEIFLAAEATNGIGDEQTAALL 435
                    +    S +D IA         +  L++F +++ +    T G+    TA ++
Sbjct: 480 SYDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIF-KDVDVIVTPTTGM----TAPVI 539

Query: 436 NLAKLTEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFGG 495
               L ++G   +        V T          + G P I+VP GYD  G+P G+   G
Sbjct: 540 PPDAL-KNGETNI-------QVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMG 598

Query: 496 LKGSEGKLIEVAYGFEQATMIRKPPS 506
              +E  ++ +A   E+   + K P+
Sbjct: 600 RPWAEATVLGLAAAVEELAPVTKKPA 598

The following BLAST results are available for this feature:

BLAST of ClCG02G004250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887424.1	5.6e-259	91.70	probable amidase At4g34880 [Benincasa hispida]	[more]
KAA0049736.1	1.8e-257	90.93	putative amidase isoform X2 [Cucumis melo var. makuwa] >TYK12141.1 putative amid...	[more]
XP_016900445.1	1.8e-257	90.73	PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo]	[more]
XP_031745278.1	4.0e-257	91.12	probable amidase At4g34880 [Cucumis sativus] >KGN43234.1 hypothetical protein Cs...	[more]
XP_022953021.1	2.5e-251	90.78	putative amidase C869.01 [Cucurbita moschata]	[more]

BLAST of ClCG02G004250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1P8B760	4.3e-169	64.45	Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=...	[more]
Q9URY4	7.8e-78	38.06	Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
D4B3C8	4.7e-67	35.66	Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ...	[more]
B0K3S3	1.9e-47	31.60	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ...	[more]
B0KBN4	9.3e-47	31.75	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol...	[more]

BLAST of ClCG02G004250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U6F5	8.8e-258	90.93	Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S4DXK2	8.8e-258	90.73	putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4...	[more]
A0A0A0K2I0	2.0e-257	91.12	Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=...	[more]
A0A6J1GLV2	1.2e-251	90.78	putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1	[more]
A0A6J1HUS8	4.0e-250	90.66	putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1	[more]

BLAST of ClCG02G004250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34880.1	8.4e-144	56.76	Amidase family protein	[more]
AT3G25660.1	7.6e-28	28.25	Amidase family protein	[more]
AT5G07360.1	2.7e-25	31.75	Amidase family protein	[more]
AT5G07360.2	5.6e-23	31.35	Amidase family protein	[more]
AT5G64440.1	1.8e-16	25.11	fatty acid amide hydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 19..506 e-value: 3.0E-141 score: 473.8
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 26..505
IPR023631	Amidase signature domain	PFAM	PF01425	Amidase	coord: 48..450 e-value: 1.7E-68 score: 231.7
None	No IPR available	PANTHER	PTHR42678:SF19	C869.01-LIKE PROTEIN, PUTATIVE-RELATED	coord: 19..508
None	No IPR available	PANTHER	PTHR42678	AMIDASE	coord: 19..508

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG02G004250.2	ClCG02G004250.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane