ClCG02G002230 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG02G002230
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionglucosidase 2 subunit beta
LocationCG_Chr02: 2279816 .. 2282695 (-)
RNA-Seq ExpressionClCG02G002230
SyntenyClCG02G002230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGCGCCTTCTTCTCCAAAGTCCACCAGCTTCGTTTCGCTGCTTTCCATCTGTCTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTCATCGGAGTTCACCCCCTCGGTACTCTTCTCTCTCTCTCTCCCTCCGTGCTTAATGCTCCCCCTGGATCTGATCTTAAAAAGCTCAAACATGGAATTTCTAGCCCCATTTCGCCCTTCCTCGAGCTAATTGCCCCTTTTGGGGTGTACCCATGTTGTGAATTGATGTTAAATTCGGTGAACTTTCGTGAATTGAAATGTGTATTTGATTGCAGATGAGAAGTATTATTCCTCCGAGGTCATAAAATGCAAGGATGGTTCTAGATCTTTCACTATTGATCGTCTCAACGATGATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTGCATTCTTAGTTCTTACTGAATTTTAATCATCAGGGTTACTGCCATTATTAGTTGAACCTCAAGTTGGAATGGCTGTGGACTTATGTATTGAACTAAGGCAACAATTACTAGCCATTGTAATCTGTGTTAAATGTGGACATAGAAAAGTTTAATGTTCTATTGTGTTAACTGTTGGAAAAGACTTACCATGTTTATCCTCGAAAAGCATGACTTGTTTTTCTATTGGAAAAGACTTACCATGTATATCCTAGAAAAGCATGACTTGTTTCTCTTTCTGAATTTTCACCAACATGTTCAGAATTGGAATGTTGATACCGTATCTATTTTGTCCGGTATCATTAGAATGTAGCCGTAGGCTAGGAAGGATAGGGAAATTTGTTAGAAATAGCACTCAAAATTTTCTACATTTTTGGCTAAATGTTTTAATGGCTCATCTCGGTATTGGTCCGGATCGATACCAAGACCAAAACAAAAATTTAACTTCTTTAAATGGCACATTCAAACGACCATTTTTTTTGGACCTTCTCAGTGGAATCCCGGTCTATACGAAGACCAAGACGGACTATTATTCTACGTAAGCTCATAAAATCTTATTCCTTCCTCATCATACTCTTTCCGATAAGATTACATACATAATATTTGGGAAAGTTTATGTAAGATTTGGGGAATACGTACATAATAATAGTTGGTCCTGATATTTGTCGTGGTTGGGATTTCACTGAAAAAAGAAAAAAATCAATGGTTTGGATGTGTCATTAAAAGAAGCTAAATTTTGTTTTGGTCTCATTATGCCAAGATGCACCCAATGGTCCATTAAAACAATTGCCCCTGTTAAAGGTGAAAAACTTTAGTACCCAGCTATTACCTACAGGATAGGTTCTGCATCAAAATGATGCTGGACTTATAAGCAATAAATCCTAATTCCGGTATGAACAATTGGTCATGCAAACAAAAGGAAAACAACCCTTCCCAAATAGCCCAAGAACAGCCCCCACACACTAACACAGTGCAAGAATCAGGCAGATTTTTTGTCTGTGCTCTGCTGCTGCTGGCTCATGGATTTTACCTTTGATCTTTGTATTTATATCTGTAGGTACTTCTGCCTGTGCAAGAGGGAAATTCTATTGCCGGAATATGGGAAGTACACCACGGTTCATATTTTCTTCCCGTGTCAACGATCATATTTGTGGTAACTATCTAATAAATCTTTTGTTTTCACTATGCACATGGCTCAATCAAATTTTGATCAAAAGAAGAAAAGCGTGTCGATTGAAACACCATAAATTTGTTGATGTAGATTTTAGAACAACCTTTCTGTATGGTGTGTGTAATGCAATGAGTATAGTCTTACGAGTCATGCGCTAGTTGTCACGATTATTTGTTCTTCCTGCTTTATGCTAAGATCATTTTGTTGAAACTTGAAAATCAGCATATTCCATAACTCTCGAAATAATCCTTGCAGACCATTTTGTGTCATGTAAAAAGTTTATAGGCATTTTTTTTACTTATGTTGAATCCACGGTTAATTGAGAAACTTGAGGTTTTCATTTAATCTTTGAAATTAAATGCTAGTTCCCTGTTCAACATATATTTGTAGATTGCTGTGATGGAAGTGATGAATATGATGGTAATATCATTTGCCCGAACACATGTGTCATGGGGGGAAATATAGTGTACAAGAGCAACAAAGATATTTCAACCACTCGAGATGTTGATATAGTCAACAGAAAAGTTAAGGAAGAAATCACCAAGGAGGACATGTTCCAAAAGCTAATCGGTAGGATTTTTGCTTCAGTAATTACAAAACACAGATACACAATACTCAACTGATTGTTGTAACATGCAATTTCAGGTCTGAAGCTAGTAACAATCCTACAGGTAGCTCTAACAAGTTTTGCAGTCCTCATTTGGGTAAACCGTTGTCGTATGAAATCTAAGAGAAGACGACACCGTTGAAGTGAGCATGACAATCGTCATTCTTCTCCGCTATAATCCCAAGTACGTCTCCCATGGCATATAATACTTTCTCTAGTCTATTCTGTTAGTCTTATTGTTTAGTGTTCAATGTCCATTCCATCTCCTGACTTTCTTATCAGCACGATTTCATGATGTTGTTTCTCAGGTGCTCACGAAGCTCAACGCACTGGATAAAGAACTCCTGCGACCTTCACACTATTTTGCAGAGTCATGCCTATCTTGAAGTTGATTTCAACAGCCTTAACAGACCGTGATCCATTGGCTACCAAAGGCTAAGTTTTATTTGTTACAATTAGGCTAGCTGATTGACTCATTAGAATAACACAAGGATTAGCAACTCAATCTCTATCAATTTTGTTACAATTAGGCTAGCTTATTGACTCATTAGTCAAATGAATGGTTTCTTCCTCAATCCTCTCCATCCGATGTATGAAACCCGCCCCCTACAACATACTATATCAAAAGGAACAATTTTGTTACA

mRNA sequence

ATGCAAGCGCCTTCTTCTCCAAAGTCCACCAGCTTCGTTTCGCTGCTTTCCATCTGTCTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTCATCGGAGTTCACCCCCTCGGTACTCTTCTCTCTCTCTCTCCCTCCGTGCTTAATGCTCCCCCTGGATCTGATCTTAAAAAGCTCAAACATGGAATTTCTAGCCCCATTTCGCCCTTCCTCGAGCTAATTGCCCCTTTTGGGGTGTACCCATGTTGTGAATTGATGTTAAATTCGGTGAACTTTCATGAGAAGTATTATTCCTCCGAGGTCATAAAATGCAAGGATGGTTCTAGATCTTTCACTATTGATCGTCTCAACGATGATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTACTTCTGCCTGTGCAAGAGGGAAATTCTATTGCCGGAATATGGGAAGTACACCACGGTTCATATTTTCTTCCCGTGTCAACGATCATATTTGTGATTGCTGTGATGGAAGTGATGAATATGATGGTAATATCATTTGCCCGAACACATGTGTCATGGGGGGAAATATAGTGTACAAGAGCAACAAAGATATTTCAACCACTCGAGATGTTGATATAGTCAACAGAAAAGTTAAGGAAGAAATCACCAAGGAGGACATGTTCCAAAAGCTAATCGGTCTGAAGCTAGTAACAATCCTACAGGTAGCTCTAACAAGTTTTGCAGTCCTCATTTGGGTAAACCGTTGTCGTATGAAATCTAAGAGAAGACGACACCGTTGAAGTGAGCATGACAATCGTCATTCTTCTCCGCTATAATCCCAAGTGCTCACGAAGCTCAACGCACTGGATAAAGAACTCCTGCGACCTTCACACTATTTTGCAGAGTCATGCCTATCTTGAAGTTGATTTCAACAGCCTTAACAGACCGTGATCCATTGGCTACCAAAGGCTAAGTTTTATTTGTTACAATTAGGCTAGCTGATTGACTCATTAGAATAACACAAGGATTAGCAACTCAATCTCTATCAATTTTGTTACAATTAGGCTAGCTTATTGACTCATTAGTCAAATGAATGGTTTCTTCCTCAATCCTCTCCATCCGATGTATGAAACCCGCCCCCTACAACATACTATATCAAAAGGAACAATTTTGTTACA

Coding sequence (CDS)

ATGCAAGCGCCTTCTTCTCCAAAGTCCACCAGCTTCGTTTCGCTGCTTTCCATCTGTCTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTCATCGGAGTTCACCCCCTCGGTACTCTTCTCTCTCTCTCTCCCTCCGTGCTTAATGCTCCCCCTGGATCTGATCTTAAAAAGCTCAAACATGGAATTTCTAGCCCCATTTCGCCCTTCCTCGAGCTAATTGCCCCTTTTGGGGTGTACCCATGTTGTGAATTGATGTTAAATTCGGTGAACTTTCATGAGAAGTATTATTCCTCCGAGGTCATAAAATGCAAGGATGGTTCTAGATCTTTCACTATTGATCGTCTCAACGATGATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTACTTCTGCCTGTGCAAGAGGGAAATTCTATTGCCGGAATATGGGAAGTACACCACGGTTCATATTTTCTTCCCGTGTCAACGATCATATTTGTGATTGCTGTGATGGAAGTGATGAATATGATGGTAATATCATTTGCCCGAACACATGTGTCATGGGGGGAAATATAGTGTACAAGAGCAACAAAGATATTTCAACCACTCGAGATGTTGATATAGTCAACAGAAAAGTTAAGGAAGAAATCACCAAGGAGGACATGTTCCAAAAGCTAATCGGTCTGAAGCTAGTAACAATCCTACAGGTAGCTCTAACAAGTTTTGCAGTCCTCATTTGGGTAAACCGTTGTCGTATGAAATCTAAGAGAAGACGACACCGTTGA

Protein sequence

MQAPSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLKHGISSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVLIWVNRCRMKSKRRRHR
Homology
BLAST of ClCG02G002230 vs. NCBI nr
Match: XP_038887466.1 (glucosidase 2 subunit beta [Benincasa hispida])

HSP 1 Score: 343.2 bits (879), Expect = 1.9e-90
Identity = 182/257 (70.82%), Postives = 189/257 (73.54%), Query Frame = 0

Query: 1   MQAPSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLK 60
           M+ P SPK TSFVSLLSIC FF SVRSVPSLIGVHPL                       
Sbjct: 1   METPRSPKPTSFVSLLSICFFFDSVRSVPSLIGVHPL----------------------- 60

Query: 61  HGISSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDF 120
                                            EKYY SEVIKCKDGS+SFTIDRLNDDF
Sbjct: 61  --------------------------------DEKYYYSEVIKCKDGSKSFTIDRLNDDF 120

Query: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNT 180
           CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVND ICDCCDGSDEYDGNI CPNT
Sbjct: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNT 180

Query: 181 CVMGGNIVYKSNKDISTTRDVD-IVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAV 240
           CVMGGNIVYKSN DISTTRDVD IVNRKV+EEIT+ED+FQKLIGLKLV ILQVALTSFA+
Sbjct: 181 CVMGGNIVYKSNNDISTTRDVDIIVNRKVQEEITEEDIFQKLIGLKLVIILQVALTSFAI 202

Query: 241 LIWVNRCRMKSKRRRHR 257
           LIW NRCR+KSKRRRHR
Sbjct: 241 LIWANRCRVKSKRRRHR 202

BLAST of ClCG02G002230 vs. NCBI nr
Match: XP_022136263.1 (glucosidase 2 subunit beta [Momordica charantia])

HSP 1 Score: 337.8 bits (865), Expect = 8.1e-89
Identity = 177/256 (69.14%), Postives = 186/256 (72.66%), Query Frame = 0

Query: 1   MQAPSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLK 60
           M+A   PKSTSFVS++SIC FFASVRSVPSLIGVHPL                       
Sbjct: 1   MEAGRPPKSTSFVSMISICFFFASVRSVPSLIGVHPL----------------------- 60

Query: 61  HGISSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDF 120
                                            EKYYSSEVIKCKDGSRSFTIDRLNDDF
Sbjct: 61  --------------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDF 120

Query: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNT 180
           CDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VND ICDCCDGSDEYDGNI CPNT
Sbjct: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNIFCPNT 180

Query: 181 CVMGGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVL 240
           CVMGGNI YKSN  ISTT+DV IVNRKVKEEITKED+FQKL+GLKLV +LQVALTSFAV 
Sbjct: 181 CVMGGNIAYKSNNYISTTQDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALTSFAVF 201

Query: 241 IWVNRCRMKSKRRRHR 257
           IWVNRCR +SKRRRHR
Sbjct: 241 IWVNRCRARSKRRRHR 201

BLAST of ClCG02G002230 vs. NCBI nr
Match: XP_004144515.1 (glucosidase 2 subunit beta [Cucumis sativus] >XP_031744257.1 glucosidase 2 subunit beta [Cucumis sativus] >KGN43490.1 hypothetical protein Csa_020313 [Cucumis sativus])

HSP 1 Score: 336.3 bits (861), Expect = 2.4e-88
Identity = 178/253 (70.36%), Postives = 184/253 (72.73%), Query Frame = 0

Query: 4   PSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLKHGI 63
           P SPKSTSF SLLSICLFF SVRS PS IGVHPL                          
Sbjct: 3   PRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPL-------------------------- 62

Query: 64  SSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDC 123
                                         EKYYSSEVIKCKDGSRSFTIDRLND+FCDC
Sbjct: 63  -----------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDNFCDC 122

Query: 124 VDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVM 183
           VDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEY+GNI CPNTCVM
Sbjct: 123 VDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVM 182

Query: 184 GGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVLIWV 243
           GGN +YKS  DISTTRDVDIV RKVKEEITKED+FQKL GLKLV ILQVALTSFA+LIW 
Sbjct: 183 GGN-MYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTSFAILIWA 199

Query: 244 NRCRMKSKRRRHR 257
           NRCR+KSKRRRHR
Sbjct: 243 NRCRVKSKRRRHR 199

BLAST of ClCG02G002230 vs. NCBI nr
Match: XP_008455474.1 (PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo] >XP_016901681.1 PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo] >KAA0059098.1 glucosidase 2 subunit beta isoform X1 [Cucumis melo var. makuwa] >TYJ99622.1 glucosidase 2 subunit beta isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 335.1 bits (858), Expect = 5.3e-88
Identity = 176/251 (70.12%), Postives = 184/251 (73.31%), Query Frame = 0

Query: 6   SPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLKHGISS 65
           SPKSTSFVSLLSICLFF SVR+ PS +GVHPL                            
Sbjct: 5   SPKSTSFVSLLSICLFFTSVRAAPSFLGVHPL---------------------------- 64

Query: 66  PISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD 125
                                       EKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD
Sbjct: 65  ---------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD 124

Query: 126 GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVMGG 185
           GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEY+GNI CPNTCVMGG
Sbjct: 125 GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGG 184

Query: 186 NIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVLIWVNR 245
           N +YKSN D+STTRDVDIV RKVKEEITKED+FQKL GLKLV ILQVALT FA+LIW NR
Sbjct: 185 N-MYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTIFAILIWANR 199

Query: 246 CRMKSKRRRHR 257
           CR+KSKRRRHR
Sbjct: 245 CRVKSKRRRHR 199

BLAST of ClCG02G002230 vs. NCBI nr
Match: XP_022952390.1 (glucosidase 2 subunit beta [Cucurbita moschata])

HSP 1 Score: 334.7 bits (857), Expect = 6.9e-88
Identity = 175/256 (68.36%), Postives = 184/256 (71.88%), Query Frame = 0

Query: 1   MQAPSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLK 60
           M+A  SPKS SFV LLS C FFASVRSVPSLIGVHPL                       
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPL----------------------- 60

Query: 61  HGISSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDF 120
                                            EKYYSSEVIKCKDGSRSFTIDRLNDDF
Sbjct: 61  --------------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDF 120

Query: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNT 180
           CDCVDGTDEPGTSACARGKFYCRNMGSTPRF+FSSRVNDHICDCCDGSDEYDGNI CPNT
Sbjct: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNIFCPNT 180

Query: 181 CVMGGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVL 240
           CVMGGN  YK++  IST+RDVD++NRKVKEEITKED+F +LIGLKLV ILQVALT FAVL
Sbjct: 181 CVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALTCFAVL 201

Query: 241 IWVNRCRMKSKRRRHR 257
           IW NRCR KSKRRRHR
Sbjct: 241 IWANRCRAKSKRRRHR 201

BLAST of ClCG02G002230 vs. ExPASy Swiss-Prot
Match: Q9FM96 (Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.4e-35
Identity = 76/133 (57.14%), Postives = 90/133 (67.67%), Query Frame = 0

Query: 94  EKYY-SSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFI 153
           EKYY SS  IKCKDGS+ FT  +LNDDFCDC DGTDEPGTSAC  GKFYCRN G +P  +
Sbjct: 45  EKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCSDGTDEPGTSACPTGKFYCRNAGHSPVIL 104

Query: 154 FSSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVYKSNKDISTTRDVDIVNRKVKEEI 213
           FSSRVND ICDCCDGSDEYDG++ C NTC   G    ++ K    T +  +V R+ + E 
Sbjct: 105 FSSRVNDGICDCCDGSDEYDGHVSCQNTCWEAGKAARENLKKKIETYNQGLVIRRQEIEQ 164

Query: 214 TKEDMFQKLIGLK 226
            K  + +    LK
Sbjct: 165 AKVGLEKDAAELK 177

BLAST of ClCG02G002230 vs. ExPASy Swiss-Prot
Match: A2WNF5 (Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.0e-33
Identity = 69/119 (57.98%), Postives = 84/119 (70.59%), Query Frame = 0

Query: 94  EKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIF 153
           E Y+   VI+C+DGS  F  D+LNDDFCDC DGTDEPGTSAC  GKFYC+N G +P  IF
Sbjct: 35  EAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDEPGTSACPEGKFYCQNAGHSPITIF 94

Query: 154 SSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVY-KSNKDISTTRD-VDIVNRKVKE 211
           SSRVND ICDCCDGSDEYD N+ C NTC   G     K  K ++T +  V I N+++++
Sbjct: 95  SSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAARDKLKKKVATYKSGVVIRNQEIQK 153

BLAST of ClCG02G002230 vs. ExPASy Swiss-Prot
Match: Q5NBP9 (Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0276800 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.0e-33
Identity = 69/119 (57.98%), Postives = 84/119 (70.59%), Query Frame = 0

Query: 94  EKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIF 153
           E Y+   VI+C+DGS  F  D+LNDDFCDC DGTDEPGTSAC  GKFYC+N G +P  IF
Sbjct: 35  EAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDEPGTSACPEGKFYCQNAGHSPITIF 94

Query: 154 SSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVY-KSNKDISTTRD-VDIVNRKVKE 211
           SSRVND ICDCCDGSDEYD N+ C NTC   G     K  K ++T +  V I N+++++
Sbjct: 95  SSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAARDKLKKKVATYKSGVVIRNQEIQK 153

BLAST of ClCG02G002230 vs. ExPASy Swiss-Prot
Match: O08795 (Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.0e-26
Identity = 59/140 (42.14%), Postives = 87/140 (62.14%), Query Frame = 0

Query: 89  SVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGST 148
           S++ H  Y  S+   C DG+ +   D++NDD+CDC DG+DEPGT+AC  G F+C N G  
Sbjct: 24  SLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYK 83

Query: 149 PRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVYKSNKDIS-TTRDVDIVNRK 208
           P +I SSRVND +CDCCDG+DEY+   +C NTC   G    +S + ++  TR+   + + 
Sbjct: 84  PLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKI 143

Query: 209 VKEE--ITKEDMFQKLIGLK 226
           + EE    +E+   KL+ L+
Sbjct: 144 LIEEWKTAREEKQSKLLELQ 163

BLAST of ClCG02G002230 vs. ExPASy Swiss-Prot
Match: P14314 (Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 2.7e-26
Identity = 61/140 (43.57%), Postives = 88/140 (62.86%), Query Frame = 0

Query: 89  SVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGST 148
           S+  H  Y  S+   C DGS +   D++NDD+CDC DG+DEPGT+AC  G F+C N G  
Sbjct: 24  SLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYK 83

Query: 149 PRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVYKSNKDIS-TTRDVDIVNRK 208
           P +I S+RVND +CDCCDG+DEY+  +IC NTC   G    +S + ++  TR+   + + 
Sbjct: 84  PLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKI 143

Query: 209 VKEEITK--EDMFQKLIGLK 226
           + E+  K  E+  +KLI L+
Sbjct: 144 LIEDWKKAREEKQKKLIELQ 163

BLAST of ClCG02G002230 vs. ExPASy TrEMBL
Match: A0A6J1C765 (glucosidase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111008003 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.9e-89
Identity = 177/256 (69.14%), Postives = 186/256 (72.66%), Query Frame = 0

Query: 1   MQAPSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLK 60
           M+A   PKSTSFVS++SIC FFASVRSVPSLIGVHPL                       
Sbjct: 1   MEAGRPPKSTSFVSMISICFFFASVRSVPSLIGVHPL----------------------- 60

Query: 61  HGISSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDF 120
                                            EKYYSSEVIKCKDGSRSFTIDRLNDDF
Sbjct: 61  --------------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDF 120

Query: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNT 180
           CDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VND ICDCCDGSDEYDGNI CPNT
Sbjct: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNIFCPNT 180

Query: 181 CVMGGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVL 240
           CVMGGNI YKSN  ISTT+DV IVNRKVKEEITKED+FQKL+GLKLV +LQVALTSFAV 
Sbjct: 181 CVMGGNIAYKSNNYISTTQDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALTSFAVF 201

Query: 241 IWVNRCRMKSKRRRHR 257
           IWVNRCR +SKRRRHR
Sbjct: 241 IWVNRCRARSKRRRHR 201

BLAST of ClCG02G002230 vs. ExPASy TrEMBL
Match: A0A0A0K1G3 (PRKCSH-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041350 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.1e-88
Identity = 178/253 (70.36%), Postives = 184/253 (72.73%), Query Frame = 0

Query: 4   PSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLKHGI 63
           P SPKSTSF SLLSICLFF SVRS PS IGVHPL                          
Sbjct: 3   PRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPL-------------------------- 62

Query: 64  SSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDC 123
                                         EKYYSSEVIKCKDGSRSFTIDRLND+FCDC
Sbjct: 63  -----------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDNFCDC 122

Query: 124 VDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVM 183
           VDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEY+GNI CPNTCVM
Sbjct: 123 VDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVM 182

Query: 184 GGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVLIWV 243
           GGN +YKS  DISTTRDVDIV RKVKEEITKED+FQKL GLKLV ILQVALTSFA+LIW 
Sbjct: 183 GGN-MYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTSFAILIWA 199

Query: 244 NRCRMKSKRRRHR 257
           NRCR+KSKRRRHR
Sbjct: 243 NRCRVKSKRRRHR 199

BLAST of ClCG02G002230 vs. ExPASy TrEMBL
Match: A0A1S4E0C8 (glucosidase 2 subunit beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495629 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.6e-88
Identity = 176/251 (70.12%), Postives = 184/251 (73.31%), Query Frame = 0

Query: 6   SPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLKHGISS 65
           SPKSTSFVSLLSICLFF SVR+ PS +GVHPL                            
Sbjct: 5   SPKSTSFVSLLSICLFFTSVRAAPSFLGVHPL---------------------------- 64

Query: 66  PISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD 125
                                       EKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD
Sbjct: 65  ---------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD 124

Query: 126 GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVMGG 185
           GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEY+GNI CPNTCVMGG
Sbjct: 125 GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGG 184

Query: 186 NIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVLIWVNR 245
           N +YKSN D+STTRDVDIV RKVKEEITKED+FQKL GLKLV ILQVALT FA+LIW NR
Sbjct: 185 N-MYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTIFAILIWANR 199

Query: 246 CRMKSKRRRHR 257
           CR+KSKRRRHR
Sbjct: 245 CRVKSKRRRHR 199

BLAST of ClCG02G002230 vs. ExPASy TrEMBL
Match: A0A5D3BIQ3 (Glucosidase 2 subunit beta isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold786G00030 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.6e-88
Identity = 176/251 (70.12%), Postives = 184/251 (73.31%), Query Frame = 0

Query: 6   SPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLKHGISS 65
           SPKSTSFVSLLSICLFF SVR+ PS +GVHPL                            
Sbjct: 5   SPKSTSFVSLLSICLFFTSVRAAPSFLGVHPL---------------------------- 64

Query: 66  PISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD 125
                                       EKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD
Sbjct: 65  ---------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVD 124

Query: 126 GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNTCVMGG 185
           GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEY+GNI CPNTCVMGG
Sbjct: 125 GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGG 184

Query: 186 NIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVLIWVNR 245
           N +YKSN D+STTRDVDIV RKVKEEITKED+FQKL GLKLV ILQVALT FA+LIW NR
Sbjct: 185 N-MYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTIFAILIWANR 199

Query: 246 CRMKSKRRRHR 257
           CR+KSKRRRHR
Sbjct: 245 CRVKSKRRRHR 199

BLAST of ClCG02G002230 vs. ExPASy TrEMBL
Match: A0A6J1GLM3 (glucosidase 2 subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111455090 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 3.3e-88
Identity = 175/256 (68.36%), Postives = 184/256 (71.88%), Query Frame = 0

Query: 1   MQAPSSPKSTSFVSLLSICLFFASVRSVPSLIGVHPLGTLLSLSPSVLNAPPGSDLKKLK 60
           M+A  SPKS SFV LLS C FFASVRSVPSLIGVHPL                       
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPL----------------------- 60

Query: 61  HGISSPISPFLELIAPFGVYPCCELMLNSVNFHEKYYSSEVIKCKDGSRSFTIDRLNDDF 120
                                            EKYYSSEVIKCKDGSRSFTIDRLNDDF
Sbjct: 61  --------------------------------DEKYYSSEVIKCKDGSRSFTIDRLNDDF 120

Query: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYDGNIICPNT 180
           CDCVDGTDEPGTSACARGKFYCRNMGSTPRF+FSSRVNDHICDCCDGSDEYDGNI CPNT
Sbjct: 121 CDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNIFCPNT 180

Query: 181 CVMGGNIVYKSNKDISTTRDVDIVNRKVKEEITKEDMFQKLIGLKLVTILQVALTSFAVL 240
           CVMGGN  YK++  IST+RDVD++NRKVKEEITKED+F +LIGLKLV ILQVALT FAVL
Sbjct: 181 CVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALTCFAVL 201

Query: 241 IWVNRCRMKSKRRRHR 257
           IW NRCR KSKRRRHR
Sbjct: 241 IWANRCRAKSKRRRHR 201

BLAST of ClCG02G002230 vs. TAIR 10
Match: AT2G42390.1 (protein kinase C substrate, heavy chain-related )

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-51
Identity = 97/167 (58.08%), Postives = 124/167 (74.25%), Query Frame = 0

Query: 94  EKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIF 153
           EKY+ S+VIKCKDGS+SFT DRLND+FCDC+DGTDEPGTSAC  GKFYCRN+GS+P+F++
Sbjct: 33  EKYFDSDVIKCKDGSKSFTRDRLNDNFCDCLDGTDEPGTSACPNGKFYCRNIGSSPKFVY 92

Query: 154 SSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNI--VYKSNKDISTTRDVDIVNRKVKEE 213
           SSRVND ICDCCDGSDEY+ +I CPNTC+MGGN+  +YK   ++ +           KE 
Sbjct: 93  SSRVNDRICDCCDGSDEYESSIHCPNTCIMGGNVNYIYKPRANLKSIHLQLGSTPHPKEF 152

Query: 214 IT---KEDMFQKLIGLKLVTILQVALTSFAVLIWVNRCRMKSKRRRH 256
            T    +DM + L G+KLV  LQ+    F V++W+ R R +SKRRR+
Sbjct: 153 YTIGNLQDMVKNLQGMKLVFALQMVFIGFLVILWMLRSRARSKRRRY 199

BLAST of ClCG02G002230 vs. TAIR 10
Match: AT5G56360.1 (calmodulin-binding protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.0e-36
Identity = 76/133 (57.14%), Postives = 90/133 (67.67%), Query Frame = 0

Query: 94  EKYY-SSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFI 153
           EKYY SS  IKCKDGS+ FT  +LNDDFCDC DGTDEPGTSAC  GKFYCRN G +P  +
Sbjct: 45  EKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCSDGTDEPGTSACPTGKFYCRNAGHSPVIL 104

Query: 154 FSSRVNDHICDCCDGSDEYDGNIICPNTCVMGGNIVYKSNKDISTTRDVDIVNRKVKEEI 213
           FSSRVND ICDCCDGSDEYDG++ C NTC   G    ++ K    T +  +V R+ + E 
Sbjct: 105 FSSRVNDGICDCCDGSDEYDGHVSCQNTCWEAGKAARENLKKKIETYNQGLVIRRQEIEQ 164

Query: 214 TKEDMFQKLIGLK 226
            K  + +    LK
Sbjct: 165 AKVGLEKDAAELK 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887466.11.9e-9070.82glucosidase 2 subunit beta [Benincasa hispida][more]
XP_022136263.18.1e-8969.14glucosidase 2 subunit beta [Momordica charantia][more]
XP_004144515.12.4e-8870.36glucosidase 2 subunit beta [Cucumis sativus] >XP_031744257.1 glucosidase 2 subun... [more]
XP_008455474.15.3e-8870.12PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo] >XP_016901681.1 ... [more]
XP_022952390.16.9e-8868.36glucosidase 2 subunit beta [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FM961.4e-3557.14Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1[more]
A2WNF51.0e-3357.98Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 P... [more]
Q5NBP91.0e-3357.98Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0276... [more]
O087952.0e-2642.14Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1[more]
P143142.7e-2643.57Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1C7653.9e-8969.14glucosidase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111008003 PE=4 S... [more]
A0A0A0K1G31.1e-8870.36PRKCSH-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041350... [more]
A0A1S4E0C82.6e-8870.12glucosidase 2 subunit beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495629 PE... [more]
A0A5D3BIQ32.6e-8870.12Glucosidase 2 subunit beta isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1GLM33.3e-8868.36glucosidase 2 subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111455090 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G42390.11.1e-5158.08protein kinase C substrate, heavy chain-related [more]
AT5G56360.11.0e-3657.14calmodulin-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR028146Glucosidase II beta subunit, N-terminalPFAMPF12999PRKCSH-likecoord: 103..188
e-value: 2.9E-29
score: 102.1
IPR039794Glucosidase II beta subunit-likePANTHERPTHR12630N-LINKED OLIGOSACCHARIDE PROCESSINGcoord: 26..254
NoneNo IPR availablePANTHERPTHR12630:SF17EXPRESSED PROTEINcoord: 26..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG02G002230.1ClCG02G002230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006491 N-glycan processing
cellular_component GO:0017177 glucosidase II complex
cellular_component GO:0016021 integral component of membrane