ClCG01G003740 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG01G003740
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	CG_Chr01: 3975009 .. 3975623 (+)
RNA-Seq Expression	ClCG01G003740
Synteny	ClCG01G003740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTCTCCTCCCTCTAAATTTGCAATGCCAAATCTCAAGATCTCACTACCAATTCTCATTATCCTTGTAGCTCTCCACAATGCGGCAACAGCAGAGTCGTCGGCGGCGACAAGCTTCATCGAGACATCCTGCAGAGTGACCCGCTACCCGGCGCTGTGCGTTCAGTCCTTGTCAAGCTACGCCACCACCATCCGCCAGAGCGGTCGTCAGCTGGCCCGAATGGCCCTGTCCGTGAGCCTCAGCAAGGCCCGTCTCGCAGCAGCCTTCGTGGCCAAACTGGGCAAAGGCGGTGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGACTGCATAGAGAATATGGGGGACAGCGTGGACAGGCTCAGCCAATCGGTGAAGGAGCTTGGGGACTTACGTCGAACAGCTGGTGGGGATTTCTTGTGGCACATGAATAACGTTCAGACTTGGGTCAGCGCCGCCCTCACTGATGAAACCACTTGCCTTGATGGCTTCGCCGGCCACCGCTTAGACGGAAAAGTCAAGGCTACGATTCGCCGGAGAATTACCCTTGTTGCTCAAATCACTAGTAATGCTCTGGCTCTTGTTAATCGCTTTGCTGACAGAAACCATTGA

mRNA sequence

ATGCTTTCTCCTCCCTCTAAATTTGCAATGCCAAATCTCAAGATCTCACTACCAATTCTCATTATCCTTGTAGCTCTCCACAATGCGGCAACAGCAGAGTCGTCGGCGGCGACAAGCTTCATCGAGACATCCTGCAGAGTGACCCGCTACCCGGCGCTGTGCGTTCAGTCCTTGTCAAGCTACGCCACCACCATCCGCCAGAGCGGTCGTCAGCTGGCCCGAATGGCCCTGTCCGTGAGCCTCAGCAAGGCCCGTCTCGCAGCAGCCTTCGTGGCCAAACTGGGCAAAGGCGGTGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGACTGCATAGAGAATATGGGGGACAGCGTGGACAGGCTCAGCCAATCGGTGAAGGAGCTTGGGGACTTACGTCGAACAGCTGGTGGGGATTTCTTGTGGCACATGAATAACGTTCAGACTTGGGTCAGCGCCGCCCTCACTGATGAAACCACTTGCCTTGATGGCTTCGCCGGCCACCGCTTAGACGGAAAAGTCAAGGCTACGATTCGCCGGAGAATTACCCTTGTTGCTCAAATCACTAGTAATGCTCTGGCTCTTGTTAATCGCTTTGCTGACAGAAACCATTGA

Coding sequence (CDS)

ATGCTTTCTCCTCCCTCTAAATTTGCAATGCCAAATCTCAAGATCTCACTACCAATTCTCATTATCCTTGTAGCTCTCCACAATGCGGCAACAGCAGAGTCGTCGGCGGCGACAAGCTTCATCGAGACATCCTGCAGAGTGACCCGCTACCCGGCGCTGTGCGTTCAGTCCTTGTCAAGCTACGCCACCACCATCCGCCAGAGCGGTCGTCAGCTGGCCCGAATGGCCCTGTCCGTGAGCCTCAGCAAGGCCCGTCTCGCAGCAGCCTTCGTGGCCAAACTGGGCAAAGGCGGTGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGACTGCATAGAGAATATGGGGGACAGCGTGGACAGGCTCAGCCAATCGGTGAAGGAGCTTGGGGACTTACGTCGAACAGCTGGTGGGGATTTCTTGTGGCACATGAATAACGTTCAGACTTGGGTCAGCGCCGCCCTCACTGATGAAACCACTTGCCTTGATGGCTTCGCCGGCCACCGCTTAGACGGAAAAGTCAAGGCTACGATTCGCCGGAGAATTACCCTTGTTGCTCAAATCACTAGTAATGCTCTGGCTCTTGTTAATCGCTTTGCTGACAGAAACCATTGA

Protein sequence

MLSPPSKFAMPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNALALVNRFADRNH

Homology

[Close]

BLAST of ClCG01G003740 vs. NCBI nr
Match: XP_038875547.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 192/204 (94.12%), Postives = 199/204 (97.55%), Query Frame = 0


Query: 1   MLSPPSKFAMPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSS 60
           +LSPPSK AM NLKISLPILIILVALHNAATAESSAATSFIE+SC+VTRYPALCVQSLSS
Sbjct: 4   ILSPPSKLAMANLKISLPILIILVALHNAATAESSAATSFIESSCKVTRYPALCVQSLSS 63

Query: 61  YATTIRQSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVD 120
           YATTIRQSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVD
Sbjct: 64  YATTIRQSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVD 123

Query: 121 RLSQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRR 180
           RL QSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG++KA IR+
Sbjct: 124 RLGQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAAIRQ 183

Query: 181 RITLVAQITSNALALVNRFADRNH 205
           RITLVAQITSNALALVNRFAD+NH
Sbjct: 184 RITLVAQITSNALALVNRFADKNH 207

[Close]

BLAST of ClCG01G003740 vs. NCBI nr
Match: KAA0039678.1 (21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 331.6 bits (849), Expect = 4.6e-87
Identity = 175/195 (89.74%), Postives = 182/195 (93.33%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           M NLKISLP+LI L+ALHNAAT   S +TSFIE+SC+VTRYPALCVQSLS+YA TIRQSG
Sbjct: 1   MANLKISLPLLITLIALHNAATT-GSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQLAR ALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL
Sbjct: 61  RQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
           GDLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG+ KA IRRRITLVAQIT
Sbjct: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVNRFAD NH
Sbjct: 181 SNALALVNRFADENH 194

[Close]

BLAST of ClCG01G003740 vs. NCBI nr
Match: XP_004147584.1 (21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cucumis sativus])

HSP 1 Score: 330.9 bits (847), Expect = 7.9e-87
Identity = 174/195 (89.23%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           M NLKISLP+LI LVALHNAAT   SAATSFIE+SC+VTRYPALCVQSLS+YA  IRQSG
Sbjct: 1   MANLKISLPLLITLVALHNAATT-GSAATSFIESSCKVTRYPALCVQSLSTYANVIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQLAR ALSVSLSKARLA+AFVAKLGKGGGMKGLEYQAVKDCIENMGD+VDRLSQSVKEL
Sbjct: 61  RQLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
           GDLR+TAG DFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG++KA IRRRITLVAQIT
Sbjct: 121 GDLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQIT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVNRFAD NH
Sbjct: 181 SNALALVNRFADENH 194

[Close]

BLAST of ClCG01G003740 vs. NCBI nr
Match: XP_008437134.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 328.9 bits (842), Expect = 3.0e-86
Identity = 174/195 (89.23%), Postives = 181/195 (92.82%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           M NLKISLP+LI L+ALHNAAT   S +TSFIE+SC+VTRYPALCVQSLS+YA TIRQSG
Sbjct: 1   MANLKISLPLLITLIALHNAATT-GSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQLAR ALSVSLSKA LAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL
Sbjct: 61  RQLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
           GDLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG+ KA IRRRITLVAQIT
Sbjct: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVNRFAD NH
Sbjct: 181 SNALALVNRFADENH 194

[Close]

BLAST of ClCG01G003740 vs. NCBI nr
Match: KAG6606163.1 (Pectinesterase inhibitor 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 305.1 bits (780), Expect = 4.7e-79
Identity = 156/195 (80.00%), Postives = 176/195 (90.26%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           MPNLKISLP L++LV LHNAA   S+ ATSFIE+SCRVTRYPALCV SLS+YAT+IRQSG
Sbjct: 1   MPNLKISLP-LLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQL + ALSVSL+K+RLAAAFV+ L KGG ++GLEYQA+KDCIENMGD+VDRL QSVKEL
Sbjct: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
            DLRRTAGGD LWH++NVQTWVSAALTDETTCLDGFA  RLDG+VKA IRR+ITLVAQ+T
Sbjct: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVN FAD+NH
Sbjct: 181 SNALALVNHFADKNH 194

[Close]

BLAST of ClCG01G003740 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.6e-45
Identity = 93/191 (48.69%), Postives = 135/191 (70.68%), Query Frame = 0


Query: 13  LKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQL 72
           +K+S   L+    L   A A ++ A  FI+TSC +T YPA+C QSLS+YA TI+ + ++L
Sbjct: 1   MKLSKSTLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQEL 60

Query: 73  ARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL 132
           A  AL VSL++ + A  F+ +L K  G+K  +Y A+ DC+E + DS+DR+S+S  E+ +L
Sbjct: 61  ASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNL 120

Query: 133 RRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNA 192
               G DF + M+NV+TWVSAALTDETTC+DGFAG  +DGK+K ++R ++  VA++TSNA
Sbjct: 121 SHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNA 180

Query: 193 LALVNRFADRN 204
           LALVN FA ++
Sbjct: 181 LALVNNFAAKH 191

[Close]

BLAST of ClCG01G003740 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.9e-44
Identity = 102/187 (54.55%), Postives = 133/187 (71.12%), Query Frame = 0


Query: 19  ILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQ-LARMAL 78
           +L I +   ++AT   S +  FI +SC+ T+YP+LCV +LS+YAT IR +  Q LA+ AL
Sbjct: 12  LLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTAL 71

Query: 79  SVSLSKARLAAAFVAKLGK-GGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL-RRT 138
            +SL++A+    FVAKL K     K  EY A+KDCIE +G+SVDRL+QSVKEL       
Sbjct: 72  IISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAV 131

Query: 139 AGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNALAL 198
           A  DF+W M+NVQTWVSAALTDETTCLDGF+   + GKVK  IR ++  VAQ+TSNALAL
Sbjct: 132 ASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALAL 191

Query: 199 VNRFADR 203
           VN+FA++
Sbjct: 192 VNQFAEK 198

[Close]

BLAST of ClCG01G003740 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.3e-39
Identity = 90/198 (45.45%), Postives = 132/198 (66.67%), Query Frame = 0


Query: 12  NLKISLPILIILVALHNAATA-----ESSAATSFIETSCRVTRYPALCVQSLSSYATTIR 71
           N ++SL + ++ ++      A     ESS  T FI+ SC  T YP  C QSLSSYA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 72  QSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSV 131
           +  R+LA  AL+VS+++A+ A  +V+++    G+   +++AV DC+E MGD+VDRLS S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 132 KELGDLRR-TAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLV 191
           KEL  L    +G DF + ++NV+TW SAALTDET C+DGF G  + G++K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 192 AQITSNALALVNRFADRN 204
           A+ TSNALAL+N FA ++
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]

BLAST of ClCG01G003740 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-37
Identity = 88/184 (47.83%), Postives = 121/184 (65.76%), Query Frame = 0


Query: 22  ILVALHNA--ATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSV 81
           IL A  +A  A   S  A +FI+ SC+ T YP +CV SL+ YA +I+ S R+LA  AL+V
Sbjct: 18  ILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNV 77

Query: 82  SLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGD 141
           ++++A+    FV +LG+   +K  E QAVKDCIE + D+VDRL+ S+ E+       G D
Sbjct: 78  TVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRD 137

Query: 142 FLW-HMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNALALVNR 201
             W HM+N QTW SAALT+  TC DGFAG  +DG+VK ++R RI  + + TSNALAL+N 
Sbjct: 138 QFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINA 197

Query: 202 FADR 203
           FA +
Sbjct: 198 FAKK 201

[Close]

BLAST of ClCG01G003740 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.8e-33
Identity = 75/173 (43.35%), Postives = 114/173 (65.90%), Query Frame = 0


Query: 28  NAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSVSLSKARLA 87
           +++T  +     +I+TSC +T Y  +C  SLS YA+TIR + ++LA +AL+++LS A+ A
Sbjct: 135 SSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSA 194

Query: 88  AAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGDFLWHMNNV 147
           + FV  +  GGG+  LE  AV DC+E +GDSV  L  S++EL  +       F   M++V
Sbjct: 195 SKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDV 254

Query: 148 QTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNALALVNRFA 201
           +TWVSAALT++ TC+DGF+   +   VK  +RR +  VA++TSNALAL+N +A
Sbjct: 255 ETWVSAALTNDDTCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of ClCG01G003740 vs. ExPASy TrEMBL
Match: A0A5A7T9V3 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00090 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.2e-87
Identity = 175/195 (89.74%), Postives = 182/195 (93.33%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           M NLKISLP+LI L+ALHNAAT   S +TSFIE+SC+VTRYPALCVQSLS+YA TIRQSG
Sbjct: 1   MANLKISLPLLITLIALHNAATT-GSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQLAR ALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL
Sbjct: 61  RQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
           GDLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG+ KA IRRRITLVAQIT
Sbjct: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVNRFAD NH
Sbjct: 181 SNALALVNRFADENH 194

[Close]

BLAST of ClCG01G003740 vs. ExPASy TrEMBL
Match: A0A0A0KR60 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.8e-87
Identity = 174/195 (89.23%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           M NLKISLP+LI LVALHNAAT   SAATSFIE+SC+VTRYPALCVQSLS+YA  IRQSG
Sbjct: 1   MANLKISLPLLITLVALHNAATT-GSAATSFIESSCKVTRYPALCVQSLSTYANVIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQLAR ALSVSLSKARLA+AFVAKLGKGGGMKGLEYQAVKDCIENMGD+VDRLSQSVKEL
Sbjct: 61  RQLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
           GDLR+TAG DFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG++KA IRRRITLVAQIT
Sbjct: 121 GDLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQIT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVNRFAD NH
Sbjct: 181 SNALALVNRFADENH 194

[Close]

BLAST of ClCG01G003740 vs. ExPASy TrEMBL
Match: A0A1S3ATE5 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.5e-86
Identity = 174/195 (89.23%), Postives = 181/195 (92.82%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           M NLKISLP+LI L+ALHNAAT   S +TSFIE+SC+VTRYPALCVQSLS+YA TIRQSG
Sbjct: 1   MANLKISLPLLITLIALHNAATT-GSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQLAR ALSVSLSKA LAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL
Sbjct: 61  RQLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
           GDLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAG RLDG+ KA IRRRITLVAQIT
Sbjct: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVNRFAD NH
Sbjct: 181 SNALALVNRFADENH 194

[Close]

BLAST of ClCG01G003740 vs. ExPASy TrEMBL
Match: A0A6J1H2L8 (pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.6e-78
Identity = 154/195 (78.97%), Postives = 174/195 (89.23%), Query Frame = 0


Query: 10  MPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSG 69
           MPNLKISLP L++LV LHNAA   S+ ATSFIE+SCRVTRYPALCV SLS+YAT+IRQS 
Sbjct: 1   MPNLKISLP-LLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSS 60

Query: 70  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 129
           RQL + ALSVSL+K+RLAAAFV+ L KGG ++GLEYQA+KDCIENMGD+VDRL QSVKEL
Sbjct: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120

Query: 130 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQIT 189
            DLRRTAGGD LWH++NVQTWVSAALTDETTCLDGFA  RLDG+VKA IRR+ITLVAQ+T
Sbjct: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAVIRRKITLVAQVT 180

Query: 190 SNALALVNRFADRNH 205
           SNALALVN FAD+ H
Sbjct: 181 SNALALVNHFADKKH 194

[Close]

BLAST of ClCG01G003740 vs. ExPASy TrEMBL
Match: A0A6J1K807 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491486 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.2e-77
Identity = 154/196 (78.57%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 9   AMPNLKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQS 68
           AMPNLKISLP L++LV LHNAA   S+ ATSFIE+SCRVTRYPALCV SLS+YAT+IRQS
Sbjct: 21  AMPNLKISLP-LLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQS 80

Query: 69  GRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKE 128
           GRQL + ALSVSL+K+R AAAFV+ L KG  ++GLEYQA+KDCIENMGD+VDRL QSVKE
Sbjct: 81  GRQLTQTALSVSLNKSRYAAAFVSNLAKGRAVRGLEYQALKDCIENMGDTVDRLRQSVKE 140

Query: 129 LGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQI 188
           L DLRRTAGGD LWH++NVQTWVSAALTDETTCLDGFA  RLDG+VKA IRR+ITLVAQ+
Sbjct: 141 LRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQV 200

Query: 189 TSNALALVNRFADRNH 205
           TSNALALVN FAD+ H
Sbjct: 201 TSNALALVNHFADKKH 215

[Close]

BLAST of ClCG01G003740 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 2.7e-45
Identity = 102/187 (54.55%), Postives = 133/187 (71.12%), Query Frame = 0


Query: 19  ILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQ-LARMAL 78
           +L I +   ++AT   S +  FI +SC+ T+YP+LCV +LS+YAT IR +  Q LA+ AL
Sbjct: 12  LLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTAL 71

Query: 79  SVSLSKARLAAAFVAKLGK-GGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL-RRT 138
            +SL++A+    FVAKL K     K  EY A+KDCIE +G+SVDRL+QSVKEL       
Sbjct: 72  IISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAV 131

Query: 139 AGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNALAL 198
           A  DF+W M+NVQTWVSAALTDETTCLDGF+   + GKVK  IR ++  VAQ+TSNALAL
Sbjct: 132 ASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALAL 191

Query: 199 VNRFADR 203
           VN+FA++
Sbjct: 192 VNQFAEK 198

[Close]

BLAST of ClCG01G003740 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 178.7 bits (452), Expect = 4.7e-45
Identity = 96/193 (49.74%), Postives = 137/193 (70.98%), Query Frame = 0


Query: 13  LKISLPILIILVALHNAATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQL 72
           L + LP+L        +++   S++ +FI +SCRVTRY  LCV+ L+++A  IR++  QL
Sbjct: 12  LLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQL 71

Query: 73  ARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL 132
           A+ AL+V+L + +    +V KL K   +K  EY AVKDC+EN+GD ++ L+QS++EL  +
Sbjct: 72  AQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQV 131

Query: 133 RRTA--GGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITS 192
            R+     +FLW ++NV+TWVSAALTDETTCLDGF G  +DG VK+ IRRR+  VA++TS
Sbjct: 132 GRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTS 191

Query: 193 NALALVNRFADRN 204
           NALALVNRFA R+
Sbjct: 192 NALALVNRFAARH 204

[Close]

BLAST of ClCG01G003740 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 3.5e-40
Identity = 92/190 (48.42%), Postives = 128/190 (67.37%), Query Frame = 0


Query: 13  LKISLPILIILVALHNAA-TAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQ 72
           L IS  + + L A   A+ T  S  A +FI++SC+ T YPALCV SLS YA  I+ S ++
Sbjct: 10  LSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKR 69

Query: 73  LARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGD 132
           LA  A++V+LS+A+    FV++L +  G+K  E +A+KDC+E M D+VDRL++SV+EL  
Sbjct: 70  LAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKL 129

Query: 133 LRRTAGGD-FLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITS 192
                  D F +HM+N QTW SAALTDE TC DGF+G  +DG++K ++R RI  V   TS
Sbjct: 130 CGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETS 189

Query: 193 NALALVNRFA 201
           NAL+L+N FA
Sbjct: 190 NALSLINAFA 199

[Close]

BLAST of ClCG01G003740 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 5.9e-40
Identity = 90/198 (45.45%), Postives = 132/198 (66.67%), Query Frame = 0


Query: 12  NLKISLPILIILVALHNAATA-----ESSAATSFIETSCRVTRYPALCVQSLSSYATTIR 71
           N ++SL + ++ ++      A     ESS  T FI+ SC  T YP  C QSLSSYA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 72  QSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSV 131
           +  R+LA  AL+VS+++A+ A  +V+++    G+   +++AV DC+E MGD+VDRLS S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 132 KELGDLRR-TAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLV 191
           KEL  L    +G DF + ++NV+TW SAALTDET C+DGF G  + G++K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 192 AQITSNALALVNRFADRN 204
           A+ TSNALAL+N FA ++
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]

BLAST of ClCG01G003740 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.5e-38
Identity = 88/184 (47.83%), Postives = 121/184 (65.76%), Query Frame = 0


Query: 22  ILVALHNA--ATAESSAATSFIETSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSV 81
           IL A  +A  A   S  A +FI+ SC+ T YP +CV SL+ YA +I+ S R+LA  AL+V
Sbjct: 18  ILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNV 77

Query: 82  SLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGD 141
           ++++A+    FV +LG+   +K  E QAVKDCIE + D+VDRL+ S+ E+       G D
Sbjct: 78  TVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRD 137

Query: 142 FLW-HMNNVQTWVSAALTDETTCLDGFAGHRLDGKVKATIRRRITLVAQITSNALALVNR 201
             W HM+N QTW SAALT+  TC DGFAG  +DG+VK ++R RI  + + TSNALAL+N 
Sbjct: 138 QFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINA 197

Query: 202 FADR 203
           FA +
Sbjct: 198 FAKK 201

The following BLAST results are available for this feature:

BLAST of ClCG01G003740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875547.1	2.0e-98	94.12	21 kDa protein-like [Benincasa hispida]	[more]
KAA0039678.1	4.6e-87	89.74	21 kDa protein-like [Cucumis melo var. makuwa]	[more]
XP_004147584.1	7.9e-87	89.23	21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cu...	[more]
XP_008437134.1	3.0e-86	89.23	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
KAG6606163.1	4.7e-79	80.00	Pectinesterase inhibitor 9, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of ClCG01G003740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	1.6e-45	48.69	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI72	3.9e-44	54.55	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	8.3e-39	45.45	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9STY5	2.1e-37	47.83	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI74	6.8e-33	43.35	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of ClCG01G003740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T9V3	2.2e-87	89.74	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G...	[more]
A0A0A0KR60	3.8e-87	89.23	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 S...	[more]
A0A1S3ATE5	1.5e-86	89.23	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1	[more]
A0A6J1H2L8	5.6e-78	78.97	pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE...	[more]
A0A6J1K807	1.2e-77	78.57	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491486 PE=4 SV=1	[more]

BLAST of ClCG01G003740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G62770.1	2.7e-45	54.55	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	4.7e-45	49.74	pectin methylesterase inhibitor 1	[more]
AT5G62350.1	3.5e-40	48.42	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	5.9e-40	45.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	1.5e-38	47.83	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 36..195 e-value: 9.8E-54 score: 194.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 39..195 e-value: 6.2E-41 score: 140.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..197 e-value: 1.0E-36 score: 123.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 35..199 e-value: 2.5E-41 score: 143.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 39..199
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 23..202
None	No IPR available	PANTHER	PTHR31080:SF207	PECTINESTERASE INHIBITOR 9	coord: 23..202
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 43..200 e-value: 3.79481E-48 score: 152.978

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG01G003740.1	ClCG01G003740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity