ClCG01G002247 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG01G002247
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionMannan endo-1,4-beta-mannosidase
LocationCG_Chr01: 2189284 .. 2192264 (-)
RNA-Seq ExpressionClCG01G002247
SyntenyClCG01G002247
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGGACAACAATGGAAAAATATTGCTTTAGCTTGTGTTTTTGTTTTGTTAACTTTTAAGGCTTATTCATTGCCTCTTTCAACAAATGGAAGATGGATCATTGACGTTACAACTGGCCAATGCATGAAGTTGATGTGTGTAAATTGGCCGGGACACATGCAAGCTATGTTGGTAGAGGGTCTTCATCTCAGACCACTTGATGATATTGCATCCTCGGTGGCAAAGTTGAGGTTTAATTGTGTGCGTTTGACATACTCAATCCACATGTTCACACGCTATGCCAATTGGACTGTTCAACAATCCTTTGAGAATTTTGATTTGAAAGAAGCCATGGTAGGCATAGCCCAAAACAATCCTTCTTTATTGAACATGACGCTAGTTCAAGCTTATGAAGCAGTGGTTGATTCACTTGCTGCACATGGAATTATGATAGTTTCTGATAATCATATAAGCCAGCCAAGATGGTGTTGTAGTGATGATGATAGCAATGGCTTCTTTGGAGACCGCTATTTTGATCCTCAAGAATGGCTACAAGGAATCAATTTGGCAGCTCAAAGCCTAAAAAACAAACCCCAAGTATGTTTTGTTTATTTGTTTTTCATAAAATTACTTTTAAAACTATAAAAAGAGGTCAGAGTTTATGACATTGAACGCAACAACATTTTAGACTTCCTAACATGAAAATTCTAGTGAATTTGAGAACTAAGAACCCTTTAGTTATTTGAAGCCCTAATTAGCAAAATCTAAAACTTCCAAAATCAATCAGCACACAACTCCAAAGAGAGTTAGATAAAGTTACTTTGTGAATGAAGACAGGAGGTGAGGAAAACTAGTTCCCTCCTAAAGATGTGAGTACTCCACCAACGAGTTAATGAATCTTCCTCGAATGTTCAAGCATGCAACCTTAGATCCAAAAATTTCAAAAAAAAAAAAATGGTGATAAATATATGTAACTATTCTTTATTAGCAATAGAGAGGAAAATTCTCATTCCAAAAATTCAAAATCCAAAATTGTTACGTACAATCTATAGACATGGCTTTAAATATAACTTCCCAAAATCATAAAGTGTGCAACATCATAGATCTTAGTAAAAAGACTAAAATATCCTTATTTAACATAATCTAGAAAGGATAAAATAAGATACAATAAAGCTGCATTTAATAATATAAATAATAACTTTGAGCTATTGATTGCTTATATCACCTTATAACATCTAAGCTACGTATTAAGTTTAATGTGTGAATTCCATTGCCATTTTTGGATAGGTCAATGAAAGACATTTTTAGATAAAGTGATAGGTTAAGGGTTATATATAAACTTTTGAAGCTAACCTTATTATTCACAAAGATATTTTATATATTAATTTAGGTTAAAATACTATTTTTGTTGTTATACTATTTTGATCATTTTATCTTGGTTTCTATATATTTAAATATAAAAATTCTTTCCCTATATTTTCACTTTAATGCTAAAACAATTTTCTATTTAAGAAAATACACCATGTCAAATGCCTTTCAAAATTTAAAGAGGGAAGAAAAATATAGACTATTTATTTTTTTTTAAAAAAAATGTTAAAATAATTTTGACTAATCTTAAAATTGATTGATAGTATAAAAGCTAAATTTGTACAATTAATAGAGATTAAAATATAACACAACTTCTATAATATAGGAACAAAATTGGTGATTAAGCCTCTATTTTGTCATTGATTTATATGATATTTTTGAGAAATTACATGATAATGAATATATAAATTTAAAGGTGGTAGCAATAAGTCTGAGAAATGAACCACGAGGACCAAATCAAAACGTGGAGATATGGTTTCAATACATGAGCCGAGGAGCTAAGCTTGTTCACCAAATTAACCCAAATGCTTTAGTAGTGGTTTCTGGATTGAGCTATGATACCGATCTAAGCTTCTTGAAGGACAAGTCGATGGGCTTCAATTTGGATAATAAGCTTGTATTTGAGGCTCATTTGTACTCCTTCACAAACAACATGGGCGATTATTGGATGTCAAAGCCATTAAACACATTTTGTACTAATGTCAACCAAGGATTTGAAGATCGAGCTGGGTTTCTTGCAAGAGGACAAAACCCGATGCCAATCTTTGTGAGTGAGCTTGGGATCGACCAAACAGGTGCAAATGAGGGTCAAAATCGATTCCTAAGTTGCTTCTTTACTTATCTTACTGAGAATGATTTTGATTGGGGCCTATGGGCGTTGCAAGGTAGCTATTATTATAGGGAAGGTGTGAAAAATGCTGAGGAAACCTTTGGCGTCTTAGATTCAAATTTTGCAAATGTCAAAAATCCGAAATTCCTTCAGAGGTTTCAGCTTATGCAAACCAAACTTCAAGGTATAAATTTTAAAAAGTGACATGTTTCTTTTTTACATTTCTTCTTTTTAATTATCTTCCGTTCATATCTTAAGAAGAAGATTAGTTTCCACATATTTGTTTTTTTTTTTAGAATTTACATTTTTTCTCTTTACTAATTAAGAATCACATGGTTGTGCTCTTTAACACAGATCCAAGCTCAAATCTCACAACGTCATTCATAATGTACTATCCTCTTAGTGGCGAATGTGTGCGCATGGACAAAAAGTATCGATTAGGAATCCGTGGCTATAAGACCTCTAATCGTTAGAGTCATGAAAAAGATGGTGCTTCAATTAAGTTGGCACTTCAATTAAGTTGGCAGGATCTATATTGTGCTTGAAAGCTGTTGGAGATGGACTCCCTCCAATTCTTTCTAAAGACTGCTCAAGCCAACAAAATGCATGGAAATATGCTTCAAATGCCAAGCTTCAACTAGCCACTACAGATGAACAAGGACAAACCTTATGTTTGCAAAAGGCTTCACATTCGCATCAAATTTTGACTAACAAGTGCATATGTCCGAATGATTCCGAATGCCAAGGAGATCCACAAAGCCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAATTAA

mRNA sequence

ATGACAGGACAACAATGGAAAAATATTGCTTTAGCTTGTGTTTTTGTTTTGTTAACTTTTAAGGCTTATTCATTGCCTCTTTCAACAAATGGAAGATGGATCATTGACGTTACAACTGGCCAATGCATGAAGTTGATGTGTGTAAATTGGCCGGGACACATGCAAGCTATGTTGGTAGAGGGTCTTCATCTCAGACCACTTGATGATATTGCATCCTCGGTGGCAAAGTTGAGGTTTAATTGTGTGCGTTTGACATACTCAATCCACATGTTCACACGCTATGCCAATTGGACTGTTCAACAATCCTTTGAGAATTTTGATTTGAAAGAAGCCATGGTAGGCATAGCCCAAAACAATCCTTCTTTATTGAACATGACGCTAGTTCAAGCTTATGAAGCAGTGGTTGATTCACTTGCTGCACATGGAATTATGATAGTTTCTGATAATCATATAAGCCAGCCAAGATGGTGTTGTAGTGATGATGATAGCAATGGCTTCTTTGGAGACCGCTATTTTGATCCTCAAGAATGGCTACAAGGAATCAATTTGGCAGCTCAAAGCCTAAAAAACAAACCCCAAGTGGTAGCAATAAGTCTGAGAAATGAACCACGAGGACCAAATCAAAACGTGGAGATATGGTTTCAATACATGAGCCGAGGAGCTAAGCTTGTTCACCAAATTAACCCAAATGCTTTAGTAGTGGTTTCTGGATTGAGCTATGATACCGATCTAAGCTTCTTGAAGGACAAGTCGATGGGCTTCAATTTGGATAATAAGCTTGTATTTGAGGCTCATTTGTACTCCTTCACAAACAACATGGGCGATTATTGGATGTCAAAGCCATTAAACACATTTTGTACTAATGTCAACCAAGGATTTGAAGATCGAGCTGGGTTTCTTGCAAGAGGACAAAACCCGATGCCAATCTTTGTGAGTGAGCTTGGGATCGACCAAACAGGTGCAAATGAGGGTCAAAATCGATTCCTAAGTTGCTTCTTTACTTATCTTACTGAGAATGATTTTGATTGGGGCCTATGGGCGTTGCAAGGTAGCTATTATTATAGGGAAGGTGTGAAAAATGCTGAGGAAACCTTTGGCGTCTTAGATTCAAATTTTGCAAATGTCAAAAATCCGAAATTCCTTCAGAGGTTTCAGCTTATGCAAACCAAACTTCAAGGATCTATATTGTGCTTGAAAGCTGTTGGAGATGGACTCCCTCCAATTCTTTCTAAAGACTGCTCAAGCCAACAAAATGCATGGAAATATGCTTCAAATGCCAAGCTTCAACTAGCCACTACAGATGAACAAGGACAAACCTTATGTTTGCAAAAGGCTTCACATTCGCATCAAATTTTGACTAACAAGTGCATATGTCCGAATGATTCCGAATGCCAAGGAGATCCACAAAGCCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAATTAA

Coding sequence (CDS)

ATGACAGGACAACAATGGAAAAATATTGCTTTAGCTTGTGTTTTTGTTTTGTTAACTTTTAAGGCTTATTCATTGCCTCTTTCAACAAATGGAAGATGGATCATTGACGTTACAACTGGCCAATGCATGAAGTTGATGTGTGTAAATTGGCCGGGACACATGCAAGCTATGTTGGTAGAGGGTCTTCATCTCAGACCACTTGATGATATTGCATCCTCGGTGGCAAAGTTGAGGTTTAATTGTGTGCGTTTGACATACTCAATCCACATGTTCACACGCTATGCCAATTGGACTGTTCAACAATCCTTTGAGAATTTTGATTTGAAAGAAGCCATGGTAGGCATAGCCCAAAACAATCCTTCTTTATTGAACATGACGCTAGTTCAAGCTTATGAAGCAGTGGTTGATTCACTTGCTGCACATGGAATTATGATAGTTTCTGATAATCATATAAGCCAGCCAAGATGGTGTTGTAGTGATGATGATAGCAATGGCTTCTTTGGAGACCGCTATTTTGATCCTCAAGAATGGCTACAAGGAATCAATTTGGCAGCTCAAAGCCTAAAAAACAAACCCCAAGTGGTAGCAATAAGTCTGAGAAATGAACCACGAGGACCAAATCAAAACGTGGAGATATGGTTTCAATACATGAGCCGAGGAGCTAAGCTTGTTCACCAAATTAACCCAAATGCTTTAGTAGTGGTTTCTGGATTGAGCTATGATACCGATCTAAGCTTCTTGAAGGACAAGTCGATGGGCTTCAATTTGGATAATAAGCTTGTATTTGAGGCTCATTTGTACTCCTTCACAAACAACATGGGCGATTATTGGATGTCAAAGCCATTAAACACATTTTGTACTAATGTCAACCAAGGATTTGAAGATCGAGCTGGGTTTCTTGCAAGAGGACAAAACCCGATGCCAATCTTTGTGAGTGAGCTTGGGATCGACCAAACAGGTGCAAATGAGGGTCAAAATCGATTCCTAAGTTGCTTCTTTACTTATCTTACTGAGAATGATTTTGATTGGGGCCTATGGGCGTTGCAAGGTAGCTATTATTATAGGGAAGGTGTGAAAAATGCTGAGGAAACCTTTGGCGTCTTAGATTCAAATTTTGCAAATGTCAAAAATCCGAAATTCCTTCAGAGGTTTCAGCTTATGCAAACCAAACTTCAAGGATCTATATTGTGCTTGAAAGCTGTTGGAGATGGACTCCCTCCAATTCTTTCTAAAGACTGCTCAAGCCAACAAAATGCATGGAAATATGCTTCAAATGCCAAGCTTCAACTAGCCACTACAGATGAACAAGGACAAACCTTATGTTTGCAAAAGGCTTCACATTCGCATCAAATTTTGACTAACAAGTGCATATGTCCGAATGATTCCGAATGCCAAGGAGATCCACAAAGCCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAATTAA

Protein sequence

MTGQQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSNFANVKNPKFLQRFQLMQTKLQGSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKLQLATTDEQGQTLCLQKASHSHQILTNKCICPNDSECQGDPQSQWFTLVPSNVHLN
Homology
BLAST of ClCG01G002247 vs. NCBI nr
Match: KAE8648503.1 (hypothetical protein Csa_009407 [Cucumis sativus])

HSP 1 Score: 894.0 bits (2309), Expect = 5.6e-256
Identity = 425/535 (79.44%), Postives = 460/535 (85.98%), Query Frame = 0

Query: 1   MTGQQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVE 60
           MTGQ WKN+ALACVFVLLTFKAYSLPLSTNGRWI++ TTGQ +KLMCVNWPGHMQAM+ E
Sbjct: 1   MTGQPWKNLALACVFVLLTFKAYSLPLSTNGRWIVEATTGQRVKLMCVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNP 120
           GLHLRPLDDIA+ VA LRFNCVRLTYSIHMFTRYAN TV+QSFENFD+KEA+ GIAQNNP
Sbjct: 61  GLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNP 120

Query: 121 SLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQG 180
           ++LNM +V+AYEAVVDSL AHG+M+VSDNHISQPRWCCS+DD NGFFGDRYF+ QEWL+G
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEG 180

Query: 181 INLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSY 240
           ++LAAQSLKNKPQVVA+SLRNEPRGPNQNVE+WFQYMS+GAKLVHQINPNALVVVSGLSY
Sbjct: 181 LSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFL 300
           DTDLSFLK++SMGFNLDNKLVFEAHLYSFTNNM DYW SKPLNTFC NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 ARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
            RGQNP+P+FVSE GI+Q GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFANVKNPKFLQRFQLMQTKLQ---------------------------- 420
            EETFGVLD+ F NVKNPKFLQ+FQLMQTKLQ                            
Sbjct: 361 DEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMYHPLSGECVQVNKKYQ 420

Query: 421 ------------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKL 480
                                   GSILCL+AVGDGLPPILSKDCSSQQ+AWKYASNAKL
Sbjct: 421 LGVSSCKTSNRWSHEQDGTPIKLVGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKL 480

Query: 481 QLATTDEQGQTLCLQKASHSHQILTNKCICPNDSECQGDPQSQWFTLVPSNVHLN 484
           QLAT DE+GQ LCLQ+ASHSHQILTNKC+CPNDSECQGDPQSQWFTLVPSNVHLN
Sbjct: 481 QLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWFTLVPSNVHLN 535

BLAST of ClCG01G002247 vs. NCBI nr
Match: XP_031739082.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical protein Csa_010408 [Cucumis sativus])

HSP 1 Score: 887.9 bits (2293), Expect = 4.0e-254
Identity = 421/535 (78.69%), Postives = 459/535 (85.79%), Query Frame = 0

Query: 1   MTGQQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVE 60
           M+GQQWKN++LACVFVLLTF+AYSLPLSTNGRWI++ TTGQ +KL+CVNWPGHMQAM+ E
Sbjct: 1   MSGQQWKNVSLACVFVLLTFEAYSLPLSTNGRWIVEATTGQRVKLICVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNP 120
           GLHL+PLDDIA+ V KLRFNCVRLTYSIHMFTRYAN TV+QSFENFDLKEA+VGIAQNNP
Sbjct: 61  GLHLKPLDDIAAMVVKLRFNCVRLTYSIHMFTRYANLTVKQSFENFDLKEAIVGIAQNNP 120

Query: 121 SLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQG 180
           ++LNM +V+AYEAVVDSL AHG+M+VSDNHISQPRWCCS+DD NGFFGDRYF+ QEWLQG
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLQG 180

Query: 181 INLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSY 240
           ++LA QSLK KPQVVA+SLRNEPRGPNQNVE+WFQYMS+G KLVHQINPNALVVVSGLSY
Sbjct: 181 LSLATQSLKTKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGTKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFL 300
           DTDLSFLK++SMGFNLDNKLVFEAHLYSFTNNMGDYW SKPLNTFC NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 ARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
            RGQNP+P+FVSE GI+Q G NEGQNRFLSCFFTYLT+NDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGVNEGQNRFLSCFFTYLTKNDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFANVKNPKFLQRFQLMQTKLQ---------------------------- 420
            EETFGVLDS F NVKNPKFLQ+FQLMQTKLQ                            
Sbjct: 361 DEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420

Query: 421 ------------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKL 480
                                   GSILCL+AVGDGLPPILSKDCSSQQ+AWKYASNAKL
Sbjct: 421 LGVSSCKTSNRWSHEQDDTPIKLAGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKL 480

Query: 481 QLATTDEQGQTLCLQKASHSHQILTNKCICPNDSECQGDPQSQWFTLVPSNVHLN 484
           QLAT DEQGQ LCLQ+ASHSHQILTNKC+CPNDSECQGDPQSQWFTLVPSNVHL+
Sbjct: 481 QLATVDEQGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWFTLVPSNVHLS 535

BLAST of ClCG01G002247 vs. NCBI nr
Match: KAE8648505.1 (hypothetical protein Csa_007897 [Cucumis sativus])

HSP 1 Score: 886.7 bits (2290), Expect = 8.9e-254
Identity = 422/532 (79.32%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 1   MTGQQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVE 60
           MTGQ WKN+ALACVFVLLTFKAYSLPLSTNGRWI++ TTGQ +KLMCVNWPGHMQAM+ E
Sbjct: 1   MTGQPWKNLALACVFVLLTFKAYSLPLSTNGRWIVEATTGQRVKLMCVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNP 120
           GLHLRPLDDIA+ VA LRFNCVRLTYSIHMFTRYAN TV+QSFENFD+KEA+ GIAQNNP
Sbjct: 61  GLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNP 120

Query: 121 SLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQG 180
           ++LNM +V+AYEAVVDSL AHG+M+VSDNHISQPRWCCS+DD NGFFGDRYF+ QEWL+G
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEG 180

Query: 181 INLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSY 240
           ++LAAQSLKNKPQVVA+SLRNEPRGPNQNVE+WFQYMS+GAKLVHQINPNALVVVSGLSY
Sbjct: 181 LSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFL 300
           DTDLSFLK++SMGFNLDNKLVFEAHLYSFTNNM DYW SKPLNTFC NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 ARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
            RGQNP+P+FVSE GI+Q GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFANVKNPKFLQRFQLMQTKLQ---------------------------- 420
            EETFGVLDS F NVKNPKFLQ+FQLMQTKLQ                            
Sbjct: 361 DEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420

Query: 421 ------------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKL 480
                                   GSILCL+AVGDGLPPILSKDCSSQQ+AWKYASNAKL
Sbjct: 421 LGVSSCKTSNRWSHEQDGTPIKLVGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKL 480

BLAST of ClCG01G002247 vs. NCBI nr
Match: XP_038874277.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 854.4 bits (2206), Expect = 4.9e-244
Identity = 408/535 (76.26%), Postives = 446/535 (83.36%), Query Frame = 0

Query: 1   MTGQQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVE 60
           MT  QWKNIAL C+FVLLTFKAYSLPLSTNGRWI+D  TGQ MKLMCVNWPGHMQ ML E
Sbjct: 1   MTKHQWKNIALVCIFVLLTFKAYSLPLSTNGRWIVDAATGQRMKLMCVNWPGHMQVMLAE 60

Query: 61  GLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNP 120
           GLHLRPLDDIA  VAKLRFNCVRLTYSIHMFTRYAN TVQQSFENFDLKEAM GIA+NNP
Sbjct: 61  GLHLRPLDDIAVLVAKLRFNCVRLTYSIHMFTRYANLTVQQSFENFDLKEAMAGIARNNP 120

Query: 121 SLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQG 180
           S+LNMTL++AY+AVVDSLA HG+M+VSDNHISQPRWCC++DD NGFFGDRYFDPQEWLQG
Sbjct: 121 SILNMTLIEAYKAVVDSLAVHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPQEWLQG 180

Query: 181 INLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSY 240
           I+LAAQSLK+K QVVA+SLRNEPRGPNQNVE+WFQYMS+GAKL+HQ NP+ALVV+SGLSY
Sbjct: 181 ISLAAQSLKSKFQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLIHQNNPDALVVISGLSY 240

Query: 241 DTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFL 300
           DTDLSFLK++S+GFNL+NKLVFEAHLYSFTNNM D W SKPLNTFC NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSLGFNLNNKLVFEAHLYSFTNNMSDSWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 ARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
            RGQNP P+FVSE GIDQTG N+GQNR+LSCFFTYL ENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPTPLFVSEFGIDQTGKNDGQNRYLSCFFTYLVENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFANVKNPKFLQRFQLMQTKLQ---------------------------- 420
           AEE+FGV D NF + KNPKFLQRFQLMQTKLQ                            
Sbjct: 361 AEESFGVSDFNFRHAKNPKFLQRFQLMQTKLQDPSSNLTASFIMYHSLSGECVRMDKKYQ 420

Query: 421 ------------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKL 480
                                    S+LCLKA+G+GLPPILSKDCSSQQ+ WKYASNAKL
Sbjct: 421 LRVSSCKTSNRWNHKEDGAPIKLVDSLLCLKALGEGLPPILSKDCSSQQSVWKYASNAKL 480

Query: 481 QLATTDEQGQTLCLQKASHSHQILTNKCICPNDSECQGDPQSQWFTLVPSNVHLN 484
           QLAT D+QGQ LCLQ  SHSHQI+TNKC+C +DS+CQGDPQSQWFTLV SNV+LN
Sbjct: 481 QLATVDKQGQALCLQSVSHSHQIVTNKCMCLDDSQCQGDPQSQWFTLVFSNVNLN 535

BLAST of ClCG01G002247 vs. NCBI nr
Match: KGN50395.1 (hypothetical protein Csa_000438 [Cucumis sativus])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 411/533 (77.11%), Postives = 447/533 (83.86%), Query Frame = 0

Query: 4   QQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLH 63
           QQWKNIAL CVFVLLTFKAYSLPLSTNGRWI+D TTGQ +KLMCVNWPGHMQ ML EGLH
Sbjct: 5   QQWKNIALVCVFVLLTFKAYSLPLSTNGRWIVDATTGQRVKLMCVNWPGHMQGMLAEGLH 64

Query: 64  LRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSLL 123
            RPLDDI S VAKLRFNCVRLTYSIHMFTR+AN TVQQSFENFD+K+AM GIAQNNPSL+
Sbjct: 65  RRPLDDIISLVAKLRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDAMAGIAQNNPSLV 124

Query: 124 NMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINL 183
           N+TLV+AY AVVDSLAAHG+M+VSDNHISQPRWCC++DD NGFFGDRYFDP+EWLQGI+L
Sbjct: 125 NLTLVEAYGAVVDSLAAHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPEEWLQGISL 184

Query: 184 AAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTD 243
           AAQSLK+K +VVA+S+RNEPRGPNQNVE WFQYMS+GAKL+HQINPNALVVVSGLSYDTD
Sbjct: 185 AAQSLKSKAEVVAMSMRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 244

Query: 244 LSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARG 303
           LSFLK++SMGFNLDNKLVFEAHLYSFTNNMGD+WMSKPLNTFC +VNQGFEDRAGFL RG
Sbjct: 245 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRG 304

Query: 304 QNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEE 363
           QNPMP+FVSE GIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE
Sbjct: 305 QNPMPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEE 364

Query: 364 TFGVLDSNFANVKNPK-FLQRFQLMQTKLQ------------------------------ 423
            FGVLDS FA  KN K FLQRFQLMQTKLQ                              
Sbjct: 365 NFGVLDSTFAKAKNSKLFLQRFQLMQTKLQDPSSNFTTSLIMYHPLSGGCVRMNKKYQLG 424

Query: 424 ----------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKLQL 483
                                 GS+LCLKA+G GLPPILS+DCSSQQ+ WKY S+AKLQL
Sbjct: 425 ISSCKTSNRWIHEQDSSPIKLAGSVLCLKAIGVGLPPILSQDCSSQQSIWKYGSSAKLQL 484

BLAST of ClCG01G002247 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 3.9e-111
Identity = 216/540 (40.00%), Postives = 299/540 (55.37%), Query Frame = 0

Query: 12  ACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLHLRPLDDIA 71
           A + +L+   ++SLPL T GRWI+D  TG  +KL CVNW GH++  L EGL+  P+  +A
Sbjct: 16  ALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVA 75

Query: 72  SSVAKLRFNCVRLTYSIHMFTR--YANWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQ 131
            +++ L FNCVRLTYSIHM TR  Y N TV Q+F   +L EA  GI  NNP LL++  V 
Sbjct: 76  HTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVA 135

Query: 132 AYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLK 191
           AY  VV +L+  G+M++ DNH+S+P+WCC+ DD NGFFGDRYF+P  W++G+ L A    
Sbjct: 136 AYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFN 195

Query: 192 NKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTDLSFLKD 251
           N P VVA+SLRNE RG       W ++M  GA  VH+ NP  LV++SGL +DTDLSFL  
Sbjct: 196 NTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPV 255

Query: 252 KSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARGQN--PM 311
             +      K+V+E H YSF    G  W +   N  C N    F+   GF+    N    
Sbjct: 256 LPVTLPFKEKIVYEGHWYSF----GVPWRTGLPNDVCKNETGRFKSNVGFVTSSANATAA 315

Query: 312 PIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYR---EGVKNAEET 371
           P+F+SE GIDQ   N+  NR+L+C   YL E D DW LW + GSYYYR   + VK+ EET
Sbjct: 316 PLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEET 375

Query: 372 FGVLDSNFANVKNPKFLQRFQLMQTKLQ-------------------------------- 431
           +G  + +++ ++NP F+ R + +Q  +Q                                
Sbjct: 376 YGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESGIGNTVHL 435

Query: 432 -----------------------GSILCLKAVGDGLPPILSKDCSSQQNA-WKYASNAKL 483
                                  GS  C+   G+GLP I++++CS+  N  W   S+A+L
Sbjct: 436 GSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQL 495

BLAST of ClCG01G002247 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 9.5e-17
Identity = 81/369 (21.95%), Postives = 153/369 (41.46%), Query Frame = 0

Query: 29  TNGRWIIDVTTGQCMKLMCVNWPG-HMQAMLVEGLHLRPLDDIASSVAKLRFNCVRLTYS 88
           T G  I+D  +G+      +NW G       + GL  R +DD+   V K  +N +RL YS
Sbjct: 41  TQGNKIVD-ESGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 89  IHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQAYEAVVDSLAAHGIMIVS 148
                        Q F++    +++      NP L+ +  +Q  + +++     GI I+ 
Sbjct: 101 ------------NQLFDSSSRPDSI--DYHKNPDLVGLNPIQIMDKLIEKAGQRGIQIIL 160

Query: 149 DNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLKNKPQVVAISLRNEPRGP- 208
           D H  +P    S   S  ++  +Y     W+    + A   KN P V+   L NEP G  
Sbjct: 161 DRH--RPG---SGGQSELWYTSQY-PESRWISDWKMLADRYKNNPTVIGADLHNEPHGQA 220

Query: 209 -----NQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDT-----------DLSFLKDK 268
                N + + W     R    +  +NPN L++V G+ ++            +L+ + + 
Sbjct: 221 SWGTGNASTD-WRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGVANY 280

Query: 269 SMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARGQNPMPIF 328
            +  ++ N++V+  H Y         W + P   F +N+   ++   G++++ QN  P+ 
Sbjct: 281 PVVLDVPNRVVYSPHDYG-PGVSSQPWFNDP--AFPSNLPAIWDQTWGYISK-QNIAPVL 340

Query: 329 VSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDS 380
           V E G      +  + ++ +    Y+  N+  +  W+L           N+ +T G+L  
Sbjct: 341 VGEFGGRNVDLSCPEGKWQNALVHYIGANNLYFTYWSLN---------PNSGDTGGLLLD 374

BLAST of ClCG01G002247 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-08
Identity = 71/299 (23.75%), Postives = 116/299 (38.80%), Query Frame = 0

Query: 9   IALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQA-MLVEGLHLRPL 68
           +ALA    L    A+S  ++ N R I+D  +G+ ++L  VN  G      ++ GL  R  
Sbjct: 10  LALATALALAAGPAFSYSIN-NSRQIVD-DSGKVVQLKGVNVFGFETGNHVMHGLWARNW 69

Query: 69  DDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSLLNMTL 128
            D+   +  L FN VRL +               +    D   A +  ++ N  L  +T 
Sbjct: 70  KDMIVQMQGLGFNAVRLPFC-------------PATLRSDTMPASIDYSR-NADLQGLTS 129

Query: 129 VQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQS 188
           +Q  + V+    A G+ ++ D+H       C+      + G   +   +WL  +   A  
Sbjct: 130 LQILDKVIAEFNARGMYVLLDHHTPD----CAGISELWYTGS--YTEAQWLADLRFVANR 189

Query: 189 LKNKPQVVAISLRNEPR-----GPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDT 248
            KN P V+ + L+NEP      G       W +   RG+  V  + P  L+ V G++ + 
Sbjct: 190 YKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGITDNP 249

Query: 249 DLSFLKDKSMGFNLD-----------NKLVFEAHLY-------------SFTNNMGDYW 278
             S       G NL            N+L+   H+Y             +F NNM   W
Sbjct: 250 VCSTNGGIFWGGNLQPLACTPLNIPANRLLLAPHVYGPDVFVQSYFNDSNFPNNMPAIW 286

BLAST of ClCG01G002247 vs. ExPASy TrEMBL
Match: A0A0A0KL32 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171770 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.3e-244
Identity = 411/533 (77.11%), Postives = 447/533 (83.86%), Query Frame = 0

Query: 4   QQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLH 63
           QQWKNIAL CVFVLLTFKAYSLPLSTNGRWI+D TTGQ +KLMCVNWPGHMQ ML EGLH
Sbjct: 5   QQWKNIALVCVFVLLTFKAYSLPLSTNGRWIVDATTGQRVKLMCVNWPGHMQGMLAEGLH 64

Query: 64  LRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSLL 123
            RPLDDI S VAKLRFNCVRLTYSIHMFTR+AN TVQQSFENFD+K+AM GIAQNNPSL+
Sbjct: 65  RRPLDDIISLVAKLRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDAMAGIAQNNPSLV 124

Query: 124 NMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINL 183
           N+TLV+AY AVVDSLAAHG+M+VSDNHISQPRWCC++DD NGFFGDRYFDP+EWLQGI+L
Sbjct: 125 NLTLVEAYGAVVDSLAAHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPEEWLQGISL 184

Query: 184 AAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTD 243
           AAQSLK+K +VVA+S+RNEPRGPNQNVE WFQYMS+GAKL+HQINPNALVVVSGLSYDTD
Sbjct: 185 AAQSLKSKAEVVAMSMRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 244

Query: 244 LSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARG 303
           LSFLK++SMGFNLDNKLVFEAHLYSFTNNMGD+WMSKPLNTFC +VNQGFEDRAGFL RG
Sbjct: 245 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRG 304

Query: 304 QNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEE 363
           QNPMP+FVSE GIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE
Sbjct: 305 QNPMPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEE 364

Query: 364 TFGVLDSNFANVKNPK-FLQRFQLMQTKLQ------------------------------ 423
            FGVLDS FA  KN K FLQRFQLMQTKLQ                              
Sbjct: 365 NFGVLDSTFAKAKNSKLFLQRFQLMQTKLQDPSSNFTTSLIMYHPLSGGCVRMNKKYQLG 424

Query: 424 ----------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKLQL 483
                                 GS+LCLKA+G GLPPILS+DCSSQQ+ WKY S+AKLQL
Sbjct: 425 ISSCKTSNRWIHEQDSSPIKLAGSVLCLKAIGVGLPPILSQDCSSQQSIWKYGSSAKLQL 484

BLAST of ClCG01G002247 vs. ExPASy TrEMBL
Match: A0A5A7TI19 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00580 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.8e-242
Identity = 410/533 (76.92%), Postives = 446/533 (83.68%), Query Frame = 0

Query: 4   QQWKNIALACVFV-LLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGL 63
           QQWKNIAL CVFV LLTFKAYSLPLSTNGRWIID TTG+ +KLMCVNW GHMQ MLVEGL
Sbjct: 5   QQWKNIALVCVFVLLLTFKAYSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGMLVEGL 64

Query: 64  HLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSL 123
           H RPLDDIA+ VAKLRFNCVRLTYSIHMFTR+AN TV+QSFENFD+K+A+VGIAQNNPS+
Sbjct: 65  HRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANVTVKQSFENFDMKDALVGIAQNNPSI 124

Query: 124 LNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGIN 183
           LN+TLV+AY AVVDSLAAHGIM+VSDNHISQPRWCC +DD NGFFGDRYFDPQEW QGI+
Sbjct: 125 LNLTLVEAYGAVVDSLAAHGIMVVSDNHISQPRWCCDNDDGNGFFGDRYFDPQEWFQGIS 184

Query: 184 LAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDT 243
           LAAQSLK+K QVVA+SLRNEPRGPNQNVE WFQYMS+GAKL+HQINPNALVVVSGLSYDT
Sbjct: 185 LAAQSLKSKAQVVAMSLRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDT 244

Query: 244 DLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLAR 303
           DLSFL+++SMGFNLDNKLVFEAHLYSFTNNM D+WMSKPLNTFC ++NQGFEDRAGFL R
Sbjct: 245 DLSFLRNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVR 304

Query: 304 GQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAE 363
           GQNP+P+FVSE GIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAE
Sbjct: 305 GQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAE 364

Query: 364 ETFGVLDSNFANVKNPK-FLQRFQLMQTKLQ----------------------------- 423
           E FGVLDSNF   KN K FLQRFQLMQTKLQ                             
Sbjct: 365 ENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPSSNFTTSFIMYHPLSGGCMRMNKKYQL 424

Query: 424 -----------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKLQ 483
                                  GSILCLKA+G GLPPILS+DCSSQQ+ WKY SNAKLQ
Sbjct: 425 GISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQDCSSQQSIWKYGSNAKLQ 484

BLAST of ClCG01G002247 vs. ExPASy TrEMBL
Match: A0A0A0KNB6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171760 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.3e-238
Identity = 401/533 (75.23%), Postives = 441/533 (82.74%), Query Frame = 0

Query: 4   QQWKNIALACVFVLLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLH 63
           QQW+NIAL CVFV L  KA SLPLSTNGRWI+D TTG  +KLMCVNW GHMQ ML EGLH
Sbjct: 5   QQWRNIALVCVFVFLISKACSLPLSTNGRWIVDATTGNRVKLMCVNWAGHMQGMLAEGLH 64

Query: 64  LRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQNNPSLL 123
           LRPLDDIA+ V K RFNCVRLTYSIHMFTR+AN TVQQSFENFD+K+A+ GIAQNNPS+L
Sbjct: 65  LRPLDDIAALVVKSRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDALAGIAQNNPSIL 124

Query: 124 NMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINL 183
           NMT+VQAY AV+DSLAAH +M+VSDNHISQPRWCC++DD NGFFGDRYFDPQEWLQGI+L
Sbjct: 125 NMTVVQAYGAVIDSLAAHRVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPQEWLQGISL 184

Query: 184 AAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTD 243
           AAQ+LK+K QVVA+SLRNEPRGPNQNVE+WFQYMS+GAKL+HQINPNALVVVSGLSYDTD
Sbjct: 185 AAQNLKSKSQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 244

Query: 244 LSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARG 303
           LSFLK++SMGFNLDNKLVFEAHLYSFTNNMGD+WMSKPLNTFC +VNQGFEDRAGFL RG
Sbjct: 245 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRG 304

Query: 304 QNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEE 363
           QNP+P+FVSE GIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE
Sbjct: 305 QNPIPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEE 364

Query: 364 TFGVLDSNFANVKNPK-FLQRFQLMQTKLQ------------------------------ 423
            FGVLDS FA  KN K FLQRFQLMQTKLQ                              
Sbjct: 365 NFGVLDSTFAKAKNSKLFLQRFQLMQTKLQDPSSNFTTSLIMYHPLSGGCVRMNKKYQLG 424

Query: 424 ----------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASNAKLQL 483
                                 GS+LCLKA+G GLPPILS+DCSSQQ+ WKY SNAKLQL
Sbjct: 425 ISSCKTSNRWIHEQDSSPIKLAGSVLCLKAIGVGLPPILSQDCSSQQSIWKYGSNAKLQL 484

BLAST of ClCG01G002247 vs. ExPASy TrEMBL
Match: A0A5D3BM14 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00610 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.2e-237
Identity = 407/535 (76.07%), Postives = 443/535 (82.80%), Query Frame = 0

Query: 1   MTGQ-QWKNIALACVFV-LLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAML 60
           M GQ Q KNIAL CVFV LLTFKA+SLPLSTNGRWIID TTGQ +KLMCVNW GHMQ ML
Sbjct: 1   MKGQHQRKNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGQRVKLMCVNWAGHMQGML 60

Query: 61  VEGLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQN 120
           VEGLH RPLDDIA+ VAKLRFNCVRLTYSIHMFTR+AN TV+QSFENFD+K+AM GIAQN
Sbjct: 61  VEGLHRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQN 120

Query: 121 NPSLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWL 180
           NPS+LN+TLV+AY AVVDSL AHGIM+VSDNHISQPRWCC ++D NGFFGDRYFDPQEW 
Sbjct: 121 NPSILNLTLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWF 180

Query: 181 QGINLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGL 240
           QGI+LAAQSLK+K QVVA+SLRNE RGPNQNVE WFQYMS+GAKL+HQINPNALVVVSGL
Sbjct: 181 QGISLAAQSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGL 240

Query: 241 SYDTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAG 300
           SYDTDLSFLK++SMGFNLDNKLVFEAHLYSFTNNM D+WMSKPLNTFC ++NQGFEDRAG
Sbjct: 241 SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAG 300

Query: 301 FLARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGV 360
           FL RGQNP+P+FVSE GIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GV
Sbjct: 301 FLVRGQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGV 360

Query: 361 KNAEETFGVLDSNFANVKNPK-FLQRFQLMQTKLQ------------------------- 420
           KNAEE FGVLDSNF   KN K FLQRFQLMQTKLQ                         
Sbjct: 361 KNAEENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPNSNFTTTFIMYHPLSGGCVRMNK 420

Query: 421 ---------------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASN 480
                                      GSILCLKA+G GLPPILS+DCSSQQ+ W+YASN
Sbjct: 421 KYQLGISSCKTSNRWSHEQDSAPIKLAGSILCLKAIGVGLPPILSQDCSSQQSIWRYASN 480

BLAST of ClCG01G002247 vs. ExPASy TrEMBL
Match: A0A1S3CT43 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504654 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.2e-237
Identity = 407/535 (76.07%), Postives = 444/535 (82.99%), Query Frame = 0

Query: 1   MTGQ-QWKNIALACVFV-LLTFKAYSLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAML 60
           M GQ Q KNIAL CVFV LLTFKA+SLPLSTNGRWIID TTG+ +KLMCVNW GHMQ ML
Sbjct: 1   MKGQHQRKNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGML 60

Query: 61  VEGLHLRPLDDIASSVAKLRFNCVRLTYSIHMFTRYANWTVQQSFENFDLKEAMVGIAQN 120
           VEGLH RPLDDIA+ VAKLRFNCVRLTYSIHMFTR+AN TV+QSFENFD+K+AM GIAQN
Sbjct: 61  VEGLHRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQN 120

Query: 121 NPSLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWL 180
           NPS+LN+TLV+AY AVVDSL AHGIM+VSDNHISQPRWCC ++D NGFFGDRYFDPQEWL
Sbjct: 121 NPSILNLTLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWL 180

Query: 181 QGINLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGL 240
           QGI+LAAQSLK+K QVVA+SLRNE RGPNQNVE WFQYMS+GAKL+HQINPNALVVVSGL
Sbjct: 181 QGISLAAQSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGL 240

Query: 241 SYDTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAG 300
           SYDTDLSFLK++SMGFNLDNKLVFEAHLYSFTNNM D+WMSKPLNTFC ++NQGFEDRAG
Sbjct: 241 SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAG 300

Query: 301 FLARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGV 360
           FL RGQNP+P+FVSE GIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GV
Sbjct: 301 FLVRGQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGV 360

Query: 361 KNAEETFGVLDSNFANVKNPK-FLQRFQLMQTKLQ------------------------- 420
           KNAEE FGVLDSNF   KN K FLQRFQLMQTKLQ                         
Sbjct: 361 KNAEENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPSSNFTTTFIMYHPLSGGCVRMNK 420

Query: 421 ---------------------------GSILCLKAVGDGLPPILSKDCSSQQNAWKYASN 480
                                      GSILCLKA+G GLPPILS+DCSSQQ+ W+YASN
Sbjct: 421 KYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQDCSSQQSIWRYASN 480

BLAST of ClCG01G002247 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.7e-106
Identity = 202/510 (39.61%), Postives = 282/510 (55.29%), Query Frame = 0

Query: 26  PLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLHLRPLDDIASSVAKLRFNCVRLT 85
           PLSTN RWIID   GQ +KL CVNWP H+Q ++ EGL  + +DD+A  +  + FNCVR T
Sbjct: 4   PLSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YSIHMFTRYA---NWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQAYEAVVDSLAAHG 145
           + + + T      N TV+QSF++  L + + G    NPS++++ L++AY+ VV  L  + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 IMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLKNKPQVVAISLRNE 205
           +M++ DNH+++P WCC  +D NGFFGD +FDP  W+ G+   A + K    VV +SLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 PRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTDLSFLKDKSMGFNLDNKLVF 265
            RGP QNV+ WF+YM +GA+ VH+ NPN LV++SGLSYDTDLSF++ + +      KLVF
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVF 243

Query: 266 EAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARGQNPMPIFVSELGIDQTGAN 325
           E H YSFTN   + W SK  N  C  + +  E+  GF  R     P+F+SE GID  G N
Sbjct: 244 ELHRYSFTNT--NTWSSKNPNEACGEILKSIENGGGFNLR---DFPVFLSEFGIDLRGKN 303

Query: 326 EGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSNFANVKNPKFLQ 385
              NR++ C   +  END DW +W LQGSYY REGV    E +G+LDS++  V++  FLQ
Sbjct: 304 VNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQ 363

Query: 386 RFQLMQTKLQG------------------------------------------------- 445
           R  L+ + LQG                                                 
Sbjct: 364 RLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNTL 423

Query: 446 ----SILCLKAVGDGLPPILSK-DCSSQQ-NAWKYASNAKLQLATTDEQGQTLCLQKASH 477
                 LCL++ G   P  LS+  CSS   + W+  S + + LA       +LCL     
Sbjct: 424 TLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLA-AKSTNNSLCLDVDET 483

BLAST of ClCG01G002247 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 379.4 bits (973), Expect = 4.3e-105
Identity = 206/522 (39.46%), Postives = 290/522 (55.56%), Query Frame = 0

Query: 15  FVLLTFKAYSLPLSTNGRWII-DVTTGQCMKLMCVNWPGHMQAMLVEGLHLRPLDDIASS 74
           +V+ TF   + P ST+ RWI+ D   G+ +KL CVNWP H++  + EGL  +PLD IA  
Sbjct: 14  YVITTF---AFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIAEK 73

Query: 75  VAKLRFNCVRLTYSIHMFTR---YANWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQA 134
           +  + FNCVRLT+ +++ T     A  TV+QS   F L EA+ G   +NP++L++ L++A
Sbjct: 74  IVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLIKA 133

Query: 135 YEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLKN 194
           ++ VV  L  H +M++ DNHISQP WCCSD+D NGFFGD++ +PQ W++G+   A    N
Sbjct: 134 FQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMFAN 193

Query: 195 -KPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTDLSFLKD 254
               VV +SLRNE RGP QN++ W++YM  GA+ VH +NPN LV+VSGL+Y TDLSFL++
Sbjct: 194 VSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFLRE 253

Query: 255 KSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARGQNPMPI 314
           +    +   K+VFE H Y F N     W    LN  C    +     +GFL   +  +P+
Sbjct: 254 RPFEVSFRRKVVFEIHWYGFWNT----WEGDNLNKICGKETEKMMKMSGFLL--EKGIPL 313

Query: 315 FVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLD 374
           FVSE GIDQ G N   N+FLSCF     + D DW LW L GSYY RE    ++E++GVLD
Sbjct: 314 FVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYGVLD 373

Query: 375 SNFANVKNPKFLQRFQLMQTKLQG------------------------------------ 434
            N+++++N   LQ    +QT   G                                    
Sbjct: 374 FNWSSIRNSTILQMISAIQTPFIGLMETQPKKIMFHPSTGLCIVRKSLFQLKLGSCNRSE 433

Query: 435 ---------------SILCLKAVGDGLPPILSKDCS-SQQNAWKYASNAKLQLATTDEQG 479
                           ILCLKA   G    L    S S  + WK  S++K+QL++  + G
Sbjct: 434 SWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKMQLSSITKNG 493

BLAST of ClCG01G002247 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 372.9 bits (956), Expect = 4.0e-103
Identity = 188/510 (36.86%), Postives = 283/510 (55.49%), Query Frame = 0

Query: 24  SLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLHLRPLDDIASSVAKLRFNCVR 83
           S PLST+ RWI+D   G  +KL+C NWP H+Q ++ EGL  +P+D +A  + ++ FNCVR
Sbjct: 32  SYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 84  LTYSIHMFTRYA---NWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQAYEAVVDSLAA 143
           LT+ + + T      N TV+QSF++  L + +VG   NNPS++++ L++AY+ VV +L  
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 144 HGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLKNKPQVVAISLR 203
           + +M++ DNH+++P WCC++DD NGFFGD++FDP  W+  +   A +      VV +SLR
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLR 211

Query: 204 NEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTDLSFLKDKSMGFNLDNKL 263
           NE RGP QNV  WF+YM +GA+ VH  N   LV++SGLS+D DLSF++ + +  +   KL
Sbjct: 212 NELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLSFTGKL 271

Query: 264 VFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGFLARGQNPMPIFVSELGIDQTG 323
           VFE H YSF++  G+ W +   N  C  V     +  G+L       P+F+SE GID+ G
Sbjct: 272 VFELHWYSFSD--GNSWAANNPNDICGRVLNRIGNGGGYLL--NQGFPLFLSEFGIDERG 331

Query: 324 ANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSNFANVKNPKF 383
            N   NR+  C   +  END DW LWAL GSYY R+G     E +GVLDS++ +V+N  F
Sbjct: 332 VNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSSF 391

Query: 384 LQRFQLMQTKLQG----------------------------------------------- 443
           LQ+   +Q+ LQG                                               
Sbjct: 392 LQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKKA 451

Query: 444 -----SILCLKAVGDGLPPILSK-DCSSQQNAWKYASNAKLQLATTDEQGQTLCLQKASH 477
                  LCL++ G   P  +++  CS+  + W+  S +++ LA+T     +LCL     
Sbjct: 452 LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLD-VDT 511

BLAST of ClCG01G002247 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 328.6 bits (841), Expect = 8.7e-90
Identity = 162/391 (41.43%), Postives = 233/391 (59.59%), Query Frame = 0

Query: 7   KNIALACVFVLLTFKAYSL------PLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVE 66
           K IAL  VF+ L     SL      PL T  RWI++   G  +KL C NWP H++ ++ E
Sbjct: 3   KAIALT-VFLFLFLSLISLTLATDYPLFTKSRWIVN-NKGHRVKLACANWPSHLKPVVAE 62

Query: 67  GLHLRPLDDIASSVAKLRFNCVRLTYSIHMF---TRYANWTVQQSFENFDLKEAMVGIAQ 126
           GL  +P+D I+  +  + FNCVRLT+ + +    T   N TV+QSFE + L   + GI  
Sbjct: 63  GLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYT 122

Query: 127 NNPSLLNMTLVQAYEAVVDSLAAHGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEW 186
           +NP ++N  L+  ++AVV SL  H +M++ DNH + P WCCS+DD + FFGD  F+P  W
Sbjct: 123 HNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLW 182

Query: 187 LQGINLAAQSLKNKPQVVAISLRNEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSG 246
           + G+   A    N   VV +SLRNE RG N   + W++YM +GA+ VH  NPN LV++SG
Sbjct: 183 MLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSG 242

Query: 247 LSYDTDLSFLKDKSMGFNLDNKLVFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRA 306
           L++D DLSFLKD+ +  +   KLV E H YSFT+  G  W S  +N FC+ +        
Sbjct: 243 LNFDADLSFLKDRPVNLSFKKKLVLELHWYSFTDGTGQ-WKSHNVNDFCSQMFSKERRTG 302

Query: 307 GFLARGQNPMPIFVSELGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREG 366
           GF+       P+F+SE G DQ G +   NR+++C   +  E D DW +WA+ G YY+REG
Sbjct: 303 GFVL--DQGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREG 362

Query: 367 VKNAEETFGVLDSNFANVKNPKFLQRFQLMQ 389
            +   E +G+LD+N+ NV N  +L+R  ++Q
Sbjct: 363 KRGVVEAYGMLDANWHNVHNYTYLRRLSVIQ 388

BLAST of ClCG01G002247 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.6e-88
Identity = 177/477 (37.11%), Postives = 260/477 (54.51%), Query Frame = 0

Query: 24  SLPLSTNGRWIIDVTTGQCMKLMCVNWPGHMQAMLVEGLHLRPLDDIASSVAKLRFNCVR 83
           S PLST  RWI+D   GQ +KL CVNWP H+Q  + EGL  +PLD I+  +  + FNCVR
Sbjct: 23  SYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSISKKIVSMGFNCVR 82

Query: 84  LTYSIHMFTR---YANWTVQQSFENFDLKEAMVGIAQNNPSLLNMTLVQAYEAVVDSLAA 143
           LT+ + + T        TV+QSFE+  L E ++GI  +NP LL++ L  A++ VV +L  
Sbjct: 83  LTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPLFNAFQEVVSNLGE 142

Query: 144 HGIMIVSDNHISQPRWCCSDDDSNGFFGDRYFDPQEWLQGINLAAQSLKNKPQVVAISLR 203
           +G+M++ DNH++ P WCC D+D + FFG  +FDP  W +G+   A   +N   V+ +SLR
Sbjct: 143 NGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATLFRNFTHVIGMSLR 202

Query: 204 NEPRGPNQNVEIWFQYMSRGAKLVHQINPNALVVVSGLSYDTDLSFLKDKSMGFNLDNKL 263
           NEPRG     ++WF++M +GA+ VH  NP  LV++SG+ +DT+LSFL+D+S+  +  +KL
Sbjct: 203 NEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTDKL 262

Query: 264 VFEAHLYSFTNNMGDYWMSKPLNTFCTNVNQGFEDRAGF-LARGQNPMPIFVSELGIDQT 323
           VFE H YSF++   D W     N FC  + +      GF L RG    P+ +SE G DQ 
Sbjct: 263 VFELHWYSFSDGR-DSWRKHNSNDFCVKIIEKVTHNGGFLLGRG---FPLILSEFGTDQR 322

Query: 324 GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGV-----KNA--EETFGVLDSNF 383
           G +   NR+++C   +  END DW +WAL G YY R G      KN     + G+  +N 
Sbjct: 323 GGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGPGLRPNKNLLFHPSTGLCVTNN 382

Query: 384 ANVKNPKFL---------QRFQLMQTKLQGSILCLKA---VGDGLPPILSKDCSSQQNAW 443
            +   P              F   +  L  + +C++A   VG  +   +   CS      
Sbjct: 383 PSDNIPTLRLGPCPKSDPWTFNPSEGILWINKMCVEAPNVVGQKVKLGVGTKCSKLGQ-- 442

Query: 444 KYASNAKLQLATTDEQGQTLCLQKASHSHQILTNKC-ICPNDSECQGDPQSQWFTLV 477
              S  K+ L+     G  LCL      + ++ N+C     D+ C  DP SQWF ++
Sbjct: 443 --ISATKMHLSFKTSNGLLLCLDVDERDNSVVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8648503.15.6e-25679.44hypothetical protein Csa_009407 [Cucumis sativus][more]
XP_031739082.14.0e-25478.69glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical... [more]
KAE8648505.18.9e-25479.32hypothetical protein Csa_007897 [Cucumis sativus][more]
XP_038874277.14.9e-24476.26glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
KGN50395.11.1e-24377.11hypothetical protein Csa_000438 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
C0HLA03.9e-11140.00Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P235489.5e-1721.95Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
P194871.2e-0823.75Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
A0A0A0KL325.3e-24477.11Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171770 PE=3... [more]
A0A5A7TI193.8e-24276.92Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0KNB64.3e-23875.23Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171760 PE=3... [more]
A0A5D3BM146.2e-23776.07Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CT436.2e-23776.07Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504654 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G26140.11.7e-10639.61Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.14.3e-10539.46Cellulase (glycosyl hydrolase family 5) protein [more]
AT1G13130.14.0e-10336.86Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.18.7e-9041.43Glycosyl hydrolase superfamily protein [more]
AT5G16700.13.6e-8837.11Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..390
e-value: 4.5E-69
score: 235.3
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 391..477
coord: 8..392
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 66..349
e-value: 3.4E-24
score: 85.8
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G002247.1ClCG01G002247.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds