ClCG01G000310 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG01G000310
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionGlucan endo-1,3-beta-glucosidase
LocationCG_Chr01: 242469 .. 244271 (+)
RNA-Seq ExpressionClCG01G000310
SyntenyClCG01G000310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCCTTTAACTCCCTCACACCTTCTTCTTGCTTACACTTCCACTCTAACCACTTTGCTCCTTCTTCTCTCCACACTCGTCCAAATCCAAGGTAATTACATCATTCATCAATGGCACACACTGTTCTGTGCTTTTCTTTTCATTTTCATATCAGCTTCCCTATTTCAATTATCATAGTTTTTCTAATTACCTTTCTTCAGGAGCCAAAGCTGGATCAATCGGAGTCAATTACGGTACGGTGGCTGACAATCTCCCTCCGCCGTCTAAGGTTGCAGACTTCCTCTTGGATTCTACAATTATAGATCGCGTCAGACTCTTCGATGCAAATCCCGACATCTTACGAGCTTTCGCTCATACTGGCATTTCAGTATCCATTACAATCCCTAACGATCAAATCCCCCGCTTAGCCAAGCCGAATTTCGCTGAAGAATGGATTAAATTCAACATCGAACCCTACATCCCTGCCACCAACATCATCCGCGTCTTAGTCGGCAATGAAGTCTTATCCACCGCCAACAAATTGCTCATCGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCCCTCACCGAAGCTTCCTTGGACCGCCGGATTCAAATCTCGACGCCGCACTCTCTCGGCATTTTGACAAACTCAAGCCCGCCGTCCACTGGAAGATTCCGGCAGGGCTACGACACACACGTTATCAAACCGATGCTGAGCTTCCTAAGAGAAACAAATTCGCCATTAATGGTGAATCCATATCCATTCTTCGCTTGCAACAATGAAAATCTAGATTACGCGCTCTTCCGGCCAAACCCCGGCGTGGTCGATTCAGATTCGGGGATTTTATACACCAACATGTTGGATGCTCAACTGGACGCGGTTTATTCCGCCATGAAAAGTTTGGGCTTTGCGGATCTGGACATTGTTATTGCCGAAACAGGCTGGCCCTCCAAGGGTGACCCGACCCAATTTGGTGTCGGCCCAAAAGAAGCTGCCGATTACAACGGGAATCTCATGCGACATGTCGTATCAGGCATGGGAACGCCGCTGATGCCGAACCGGACTTTCGAGACGTACATTTTCGCTCTATTCAATGAGGATCTCAAACCCGGTCCAGTTGGCGAGAGGAACTTCGGCCTTTTCGAACCGGATCTCAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGTAGCTGCCACAGCCCGAACCCACCCTAAAACATTTTTGATAATCATGATCGTTACATGTTGGTTGATTGGTTTGTTGTCGACTTGGATCTAACAGGCTCAATCCTCTACTCCGAGAGCCCATCACGGCCCAGCATCGACATCAAGAAGCCCGAGCCCAAGCCCAATCCCAATGAGTGAAGATAAAAGGTGGTGCCTTCCCAAAACTGGGGCCGACTTGGAGGCCTTGCAAAGAAATATAGATTACGTTTGTGGGTTGGGCTTGGATTGTGAGCCGATTAAAGAAAATGGGTCATGTTATGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAATGATTCTGATTGTGATTTTGACCAAACGGGTGCCCTAACTACGGTGGATCCAAGTAAGTCCTTAATAATTAATTTTTTTTAAAAATAAAAAAATAAAAAAATAAAAAAAACCAAAAGTTTGGTCATTATAAAAATTGTATGGGCATAATTAATCAAAATTTTGTAATTTAATCTTTTTATAAAAGTATTTTATTTAGCTATTTATTTTGGTTTAATTTTTCAGGTTATGGGAAGTGCAAATATTGGTAA

mRNA sequence

ATGGCTTCCCCTTTAACTCCCTCACACCTTCTTCTTGCTTACACTTCCACTCTAACCACTTTGCTCCTTCTTCTCTCCACACTCGTCCAAATCCAAGGAGCCAAAGCTGGATCAATCGGAGTCAATTACGGTACGGTGGCTGACAATCTCCCTCCGCCGTCTAAGGTTGCAGACTTCCTCTTGGATTCTACAATTATAGATCGCGTCAGACTCTTCGATGCAAATCCCGACATCTTACGAGCTTTCGCTCATACTGGCATTTCAGTATCCATTACAATCCCTAACGATCAAATCCCCCGCTTAGCCAAGCCGAATTTCGCTGAAGAATGGATTAAATTCAACATCGAACCCTACATCCCTGCCACCAACATCATCCGCGTCTTAGTCGGCAATGAAGTCTTATCCACCGCCAACAAATTGCTCATCGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCCCTCACCGAAGCTTCCTTGGACCGCCGGATTCAAATCTCGACGCCGCACTCTCTCGGCATTTTGACAAACTCAAGCCCGCCGTCCACTGGAAGATTCCGGCAGGGCTACGACACACACGTTATCAAACCGATGCTGAGCTTCCTAAGAGAAACAAATTCGCCATTAATGGTGAATCCATATCCATTCTTCGCTTGCAACAATGAAAATCTAGATTACGCGCTCTTCCGGCCAAACCCCGGCGTGGTCGATTCAGATTCGGGGATTTTATACACCAACATGTTGGATGCTCAACTGGACGCGGTTTATTCCGCCATGAAAAGTTTGGGCTTTGCGGATCTGGACATTGTTATTGCCGAAACAGGCTGGCCCTCCAAGGGTGACCCGACCCAATTTGGTGTCGGCCCAAAAGAAGCTGCCGATTACAACGGGAATCTCATGCGACATGTCGTATCAGGCATGGGAACGCCGCTGATGCCGAACCGGACTTTCGAGACGTACATTTTCGCTCTATTCAATGAGGATCTCAAACCCGGTCCAGTTGGCGAGAGGAACTTCGGCCTTTTCGAACCGGATCTCAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGCTCAATCCTCTACTCCGAGAGCCCATCACGGCCCAGCATCGACATCAAGAAGCCCGAGCCCAAGCCCAATCCCAATGAGTGAAGATAAAAGGTGGTGCCTTCCCAAAACTGGGGCCGACTTGGAGGCCTTGCAAAGAAATATAGATTACGTTTGTGGGTTGGGCTTGGATTGTGAGCCGATTAAAGAAAATGGGTCATGTTATGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAATGATTCTGATTGTGATTTTGACCAAACGGGTGCCCTAACTACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Coding sequence (CDS)

ATGGCTTCCCCTTTAACTCCCTCACACCTTCTTCTTGCTTACACTTCCACTCTAACCACTTTGCTCCTTCTTCTCTCCACACTCGTCCAAATCCAAGGAGCCAAAGCTGGATCAATCGGAGTCAATTACGGTACGGTGGCTGACAATCTCCCTCCGCCGTCTAAGGTTGCAGACTTCCTCTTGGATTCTACAATTATAGATCGCGTCAGACTCTTCGATGCAAATCCCGACATCTTACGAGCTTTCGCTCATACTGGCATTTCAGTATCCATTACAATCCCTAACGATCAAATCCCCCGCTTAGCCAAGCCGAATTTCGCTGAAGAATGGATTAAATTCAACATCGAACCCTACATCCCTGCCACCAACATCATCCGCGTCTTAGTCGGCAATGAAGTCTTATCCACCGCCAACAAATTGCTCATCGCCAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCCCTCACCGAAGCTTCCTTGGACCGCCGGATTCAAATCTCGACGCCGCACTCTCTCGGCATTTTGACAAACTCAAGCCCGCCGTCCACTGGAAGATTCCGGCAGGGCTACGACACACACGTTATCAAACCGATGCTGAGCTTCCTAAGAGAAACAAATTCGCCATTAATGGTGAATCCATATCCATTCTTCGCTTGCAACAATGAAAATCTAGATTACGCGCTCTTCCGGCCAAACCCCGGCGTGGTCGATTCAGATTCGGGGATTTTATACACCAACATGTTGGATGCTCAACTGGACGCGGTTTATTCCGCCATGAAAAGTTTGGGCTTTGCGGATCTGGACATTGTTATTGCCGAAACAGGCTGGCCCTCCAAGGGTGACCCGACCCAATTTGGTGTCGGCCCAAAAGAAGCTGCCGATTACAACGGGAATCTCATGCGACATGTCGTATCAGGCATGGGAACGCCGCTGATGCCGAACCGGACTTTCGAGACGTACATTTTCGCTCTATTCAATGAGGATCTCAAACCCGGTCCAGTTGGCGAGAGGAACTTCGGCCTTTTCGAACCGGATCTCAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGCTCAATCCTCTACTCCGAGAGCCCATCACGGCCCAGCATCGACATCAAGAAGCCCGAGCCCAAGCCCAATCCCAATGAGTGAAGATAAAAGGTGGTGCCTTCCCAAAACTGGGGCCGACTTGGAGGCCTTGCAAAGAAATATAGATTACGTTTGTGGGTTGGGCTTGGATTGTGAGCCGATTAAAGAAAATGGGTCATGTTATGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAATGATTCTGATTGTGATTTTGACCAAACGGGTGCCCTAACTACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Protein sequence

MASPLTPSHLLLAYTSTLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW
Homology
BLAST of ClCG01G000310 vs. NCBI nr
Match: XP_038906871.1 (glucan endo-1,3-beta-glucosidase [Benincasa hispida])

HSP 1 Score: 891.7 bits (2303), Expect = 2.7e-255
Identity = 440/479 (91.86%), Postives = 452/479 (94.36%), Query Frame = 0

Query: 1   MASPLTPSHLLLAYTSTLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFL 60
           MASP TPSHLL  + ST++T LLLL TL+QIQGAK GSIGVNYGT+ADNLPPPSKVA FL
Sbjct: 30  MASPFTPSHLLFVHISTVSTFLLLLPTLIQIQGAKDGSIGVNYGTLADNLPPPSKVAAFL 89

Query: 61  LDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIP 120
           LDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIP
Sbjct: 90  LDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIP 149

Query: 121 ATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSS 180
           ATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGIL+NSS
Sbjct: 150 ATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILSNSS 209

Query: 181 PPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDSD 240
           PPSTGRFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFAC  ENLDYALFRPNPGV DSD
Sbjct: 210 PPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVFDSD 269

Query: 241 SGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNL 300
           SGILYTNMLDAQLDAVYSAMKSLGF+DLDIVIAETGWPSKGD  Q GVGPKEAADYNGNL
Sbjct: 270 SGILYTNMLDAQLDAVYSAMKSLGFSDLDIVIAETGWPSKGDQAQIGVGPKEAADYNGNL 329

Query: 301 MRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTA 360
           MRHVVSG GTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTA
Sbjct: 330 MRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTA 389

Query: 361 QSSTPRAHHGP----ASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLD 420
           QSSTP+AH GP     ST RSPSPSP P+SE KRWCLPKTGAD EALQRNIDYVCGLGLD
Sbjct: 390 QSSTPKAHQGPIEGSVSTPRSPSPSPSPVSEGKRWCLPKTGADSEALQRNIDYVCGLGLD 449

Query: 421 CEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           C PI+ENG C+APNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW
Sbjct: 450 CGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 508

BLAST of ClCG01G000310 vs. NCBI nr
Match: XP_008437641.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo])

HSP 1 Score: 838.2 bits (2164), Expect = 3.6e-239
Identity = 422/482 (87.55%), Postives = 440/482 (91.29%), Query Frame = 0

Query: 1   MAS-PLTPS-HLLLAYTSTLTT-LLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVA 60
           MAS P TPS HLLL Y STL T LLL+L TL+QIQG   GSIGVNYGTVADNLPPPSKVA
Sbjct: 20  MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 79

Query: 61  DFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEP 120
            FLLD+TIIDRVRLFDA+PDILRAFAHTGISVSITIPNDQIPRL KPNFAEEWIKFNI+P
Sbjct: 80  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 139

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILT 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL EASLDRRIQISTPHSLGIL+
Sbjct: 140 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 199

Query: 181 NSSPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVV 240
           NS+PPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFAC  ENLDYALFRPNPGV 
Sbjct: 200 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 259

Query: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYN 300
           DSD GILYTNMLDAQLDAVYSAMKS+ F DLDIVIAETGWPSKGDP+Q GVGPKEAA YN
Sbjct: 260 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 319

Query: 301 GNLMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDL+PVYEIGILR
Sbjct: 320 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 379

Query: 361 PTAQSSTPRAHHGPA----STSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGL 420
           PTAQS+TPRAH GP     ST RSPS S +  SE KRWC+PKT A +EALQRNIDYVCGL
Sbjct: 380 PTAQSATPRAHQGPVEGPISTPRSPSRSTV--SESKRWCVPKTEASVEALQRNIDYVCGL 439

Query: 421 GLDCEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCK 476
           GLDC PI+ENG CYAPNTVR HAAYVMNAYFQATEG + DCDFDQTGALTTVDPSYGKC+
Sbjct: 440 GLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDPSYGKCR 499

BLAST of ClCG01G000310 vs. NCBI nr
Match: XP_011654592.1 (glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus])

HSP 1 Score: 834.3 bits (2154), Expect = 5.1e-238
Identity = 417/476 (87.61%), Postives = 432/476 (90.76%), Query Frame = 0

Query: 2   ASPLTPS-HLLLAYTSTLTT-LLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADF 61
           +SP T S HLLL Y STL T LLL+L TL QIQG   GSIGVNYGTVADNLPPPSKVA F
Sbjct: 23  SSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAF 82

Query: 62  LLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYI 121
           LLD+TIIDRVRLFDA+PDILRAFAHTGISVSITIPNDQIPRL KPNFAEEWIKFNI+PYI
Sbjct: 83  LLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYI 142

Query: 122 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNS 181
           PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL EASLDRRIQISTPHSLGIL+NS
Sbjct: 143 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNS 202

Query: 182 SPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDS 241
           +PPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFAC  +NLDYALFRPNPGV DS
Sbjct: 203 TPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDS 262

Query: 242 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGN 301
           D GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPTQ GVGPKEAA YNGN
Sbjct: 263 DLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGN 322

Query: 302 LMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPT 361
           LMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDLSPVYEIGILRPT
Sbjct: 323 LMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPT 382

Query: 362 AQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEP 421
           AQS+TPRAH G         PSP  +SE KRWCLPK+ A  E LQRNIDYVCGLGLDC P
Sbjct: 383 AQSATPRAHQGQVE-----GPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGP 442

Query: 422 IKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           IKENG+C+APNTVRAHAAYVMNAYFQATEGND DCDFDQTG LTTVDPSYGKCKYW
Sbjct: 443 IKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKYW 493

BLAST of ClCG01G000310 vs. NCBI nr
Match: XP_023521611.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.9 bits (2153), Expect = 6.7e-238
Identity = 410/476 (86.13%), Postives = 435/476 (91.39%), Query Frame = 0

Query: 1   MASPLTPSHLLLAYTS-TLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADF 60
           MAS    S LL AYTS   T LLLLL TL+ IQ AK GSIGVNYGT+ADNLP PSKVA+F
Sbjct: 16  MASSFALSRLLRAYTSIAATLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPLPSKVAEF 75

Query: 61  LLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYI 120
           LLDSTIIDR+RLFDANPDILRAFAHT ISV+IT+PNDQIPRL KPNFAEEWIKFN++PY+
Sbjct: 76  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 135

Query: 121 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNS 180
           PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL+EASL RRIQ+STPHSLGIL+NS
Sbjct: 136 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHSLGILSNS 195

Query: 181 SPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDS 240
           SPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFF    E LDYALFRPNPGV DS
Sbjct: 196 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 255

Query: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGN 300
           DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDP Q GVGPKEAADYNGN
Sbjct: 256 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 315

Query: 301 LMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSG+GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILRPT
Sbjct: 316 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 375

Query: 361 AQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEP 420
           AQSSTPRAHHGP  T + PSPSP P+++ K WCLPKT AD EALQRNIDYVCGLGLDC P
Sbjct: 376 AQSSTPRAHHGPEPTPKGPSPSPSPVADGKTWCLPKTEADSEALQRNIDYVCGLGLDCGP 435

Query: 421 IKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           I+ENG C+ PNTVRAHAAYVMNAYFQAT+GNDSDCDF QTG LTTVDPSYGKCKYW
Sbjct: 436 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKYW 490

BLAST of ClCG01G000310 vs. NCBI nr
Match: XP_023533272.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.9 bits (2153), Expect = 6.7e-238
Identity = 410/476 (86.13%), Postives = 435/476 (91.39%), Query Frame = 0

Query: 1   MASPLTPSHLLLAYTS-TLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADF 60
           MAS    S LL AYTS   T LLLLL TL+ IQ AK GSIGVNYGT+ADNLP PSKVA+F
Sbjct: 1   MASSFALSRLLRAYTSIAATLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPLPSKVAEF 60

Query: 61  LLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYI 120
           LLDSTIIDR+RLFDANPDILRAFAHT ISV+IT+PNDQIPRL KPNFAEEWIKFN++PY+
Sbjct: 61  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 120

Query: 121 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNS 180
           PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL+EASL RRIQ+STPHSLGIL+NS
Sbjct: 121 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHSLGILSNS 180

Query: 181 SPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDS 240
           SPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFF    E LDYALFRPNPGV DS
Sbjct: 181 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 240

Query: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGN 300
           DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDP Q GVGPKEAADYNGN
Sbjct: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 300

Query: 301 LMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSG+GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILRPT
Sbjct: 301 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360

Query: 361 AQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEP 420
           AQSSTPRAHHGP  T + PSPSP P+++ K WCLPKT AD EALQRNIDYVCGLGLDC P
Sbjct: 361 AQSSTPRAHHGPEPTPKGPSPSPSPVADGKTWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 421 IKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           I+ENG C+ PNTVRAHAAYVMNAYFQAT+GNDSDCDF QTG LTTVDPSYGKCKYW
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKYW 475

BLAST of ClCG01G000310 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 5.3e-129
Identity = 233/464 (50.22%), Postives = 316/464 (68.10%), Query Frame = 0

Query: 21  LLLLLSTLVQIQGAKAGS----IGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANP 80
           L+LL+       GA++ +    IGVNYG  ADNLP P+ VA FL   T IDRV+LFDANP
Sbjct: 4   LILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANP 63

Query: 81  DILRAFAHTGISVSITIPNDQIPRLAKP----NFAEEWIKFNIEPYIPATNIIRVLVGNE 140
             + AFA T IS+++++PN  +P LA      + A  WI+ N+ PY+PATN+  +L GNE
Sbjct: 64  TFISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNE 123

Query: 141 VLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSP-PSTGRFRQGY 200
           +L + +  LI +L+PAM+ L  AL    L   ++++TPH LGIL  S   PS   FR GY
Sbjct: 124 ILLSTDTNLILSLLPAMRRLAQALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGY 183

Query: 201 DTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDSDSGILYTNMLDA 260
           +T +   ML F R+T SP MVNPYP+F+   E L+YALFRPN G+ D  + + YT+MLDA
Sbjct: 184 NTKLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDA 243

Query: 261 QLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTP 320
           Q+DA+Y+AMK LG+ D+DI + E GWP++ +P Q GVG +EA D+N  ++R   SG GTP
Sbjct: 244 QMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTP 303

Query: 321 LMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQ-SSTPRAHHG 380
           LMPNRTFETY+F+LF+E+ KPGP+ ER+FGLF PD +PVY++G+LR  A  + TP  +  
Sbjct: 304 LMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPS 363

Query: 381 PASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPN 440
           P     +PSP P P S   +WC+ K GA+   LQ NI+Y CG  +DC+PI+  G+C++PN
Sbjct: 364 P-----NPSPKPAP-SGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPN 423

Query: 441 TVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
           +++AHA+YVMNAY+QA    D  CDF  TG +T+ DPSYG CKY
Sbjct: 424 SLQAHASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of ClCG01G000310 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 419.1 bits (1076), Expect = 6.8e-116
Identity = 211/441 (47.85%), Postives = 289/441 (65.53%), Query Frame = 0

Query: 39  IGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQI 98
           IGVNYG VADNLPPPS+    LL ST I +VRL+ A+P I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 99  PRLAK-PNFAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALT 158
           P LA  PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 159 EASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 218
             SL  +I++ST +S+ +L +S PPS+G F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 219 FACNN----ENLDYALFRPNPGVVDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 278
           FA  +    E L + LF PN G VDS +GI YTNM DAQ+DAV+SA+KS+GF  ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 279 ETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPG 338
           ETGW S+GD  + G     A  YNGNL+ H+ S +GTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 339 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCL 398
           P  ER FGLF+ DLS VY++G+ + ++ S TP    G  ++S               WC+
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP---SGKVTSS--------------GWCV 385

Query: 399 PKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSD 458
           PK GA  E LQ ++D+ CG G+DC  I+  G+C+ PN V +HAAY MN YFQ +    +D
Sbjct: 386 PKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTD 445

Query: 459 CDFDQTGALTTVDPSYGKCKY 475
           CDF +T  +T+ +PSY  C Y
Sbjct: 446 CDFSKTATVTSQNPSYNNCVY 447

BLAST of ClCG01G000310 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.6e-112
Identity = 207/470 (44.04%), Postives = 299/470 (63.62%), Query Frame = 0

Query: 11  LLAYTSTLTTLLLLLSTLVQIQGAKAGS-IGVNYGTVADNLPPPSKVADFLLDSTIIDRV 70
           + A   T + L L+   L     A + S +GVNYG ++DNLP      + LL ST I +V
Sbjct: 1   MAANVQTSSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVN-LLKSTTIQKV 60

Query: 71  RLFDANPDILRAFAHTGISVSITIPNDQIPRLA-KPNFAEEWIKFNIEPYIPATNIIRVL 130
           RLF A P +++AFA+TG+ + I   N  IP LA  PN A +++K N+  + PA+NII + 
Sbjct: 61  RLFGAEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAIT 120

Query: 131 VGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFR 190
           VGNEVL++ ++ LI+ L+PAMQ++  AL  ASL  ++++ST H++ +L+ S PPS+G F 
Sbjct: 121 VGNEVLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFN 180

Query: 191 QGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNN----ENLDYALFRPNPGVVDSDSGIL 250
            G     +K +L F    ++P M++PYP+FA  N    + L + LF+PN G VDS +G  
Sbjct: 181 PGLG-DTMKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHK 240

Query: 251 YTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHV 310
           YTNM DAQ+DAV+SA+ ++GF D++IV+AETGWP  GD  + G     A  Y GNL+ H+
Sbjct: 241 YTNMFDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHL 300

Query: 311 VSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSST 370
            S +GTPLMP ++ +TY+F+L++ED K G   E+ FGLF+PD S  Y++G+L+ T   +T
Sbjct: 301 KSKVGTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTT 360

Query: 371 PRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENG 430
           P             +P+P P +    WC+PK G   + L  NI+Y CG G+DC PI+  G
Sbjct: 361 P------------ATPTPTPKAAGS-WCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGG 420

Query: 431 SCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
           +C+ PNTV+AHAAYVMN Y+Q+   N  +CDF QT  LT  +PSYG C +
Sbjct: 421 ACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNF 455

BLAST of ClCG01G000310 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.3e-92
Identity = 182/471 (38.64%), Postives = 276/471 (58.60%), Query Frame = 0

Query: 23  LLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANPDILRAF 82
           + +S L  +    A  IG+ YG  ADNLP P++V++ L+    I  VR++DAN D+L+AF
Sbjct: 11  IFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSE-LIQHLNIKFVRIYDANIDVLKAF 70

Query: 83  AHTGISVSITIPNDQIPRLAK-PNFAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANKLL 142
           A+TGI + I +PN  +   A+  +  + W+  NI PY P+T I  + VG EV    +   
Sbjct: 71  ANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-A 130

Query: 143 IANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVIKPMLS 202
              ++PAM+++HTAL ++ LD++I+IS+ HSL IL+ S PPS+  F + +    +KPML 
Sbjct: 131 TGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLKPMLE 190

Query: 203 FLRETNSPLMVNPYPFFACNNEN----LDYALFRPNPGVVDSDSGILYTNMLDAQLDAVY 262
           FL E  SP M++ YP++A  +      L+YALF  +  VVD  +G+LY+NM DAQLDA+Y
Sbjct: 191 FLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIY 250

Query: 263 SAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLMPNRT 322
            A+ ++ F  + +++ E+GWPSKG P +    P+ A  YN NL+RHV+   GTP  P   
Sbjct: 251 FALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEE 310

Query: 323 FETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTP-RAHHGPASTSR 382
            + Y+F+LFNE+ KPG   ERN+G+F  + + VY +     T +++TP    +    TS 
Sbjct: 311 IDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDF---TGENTTPVSPTNSTTGTSP 370

Query: 383 SPSPSPIPMSED------------KRWCLPKTGADLEALQRNIDYVCGLG-LDCEPIKEN 442
           SPS SPI                 K+WC+  + A +  LQ  +D+ CG G +DC  ++ +
Sbjct: 371 SPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPD 430

Query: 443 GSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
             C+ P+TV +HA+Y  N Y+Q +  +  DC F+        DPSYG C Y
Sbjct: 431 QPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of ClCG01G000310 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 1.4e-89
Identity = 185/463 (39.96%), Postives = 268/463 (57.88%), Query Frame = 0

Query: 21  LLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANPDILR 80
           LLL L        ++   IGVN GT   N+P P++V   LL S  I+RVRL+DA+  +L 
Sbjct: 5   LLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVV-ALLKSQNINRVRLYDADRSMLL 64

Query: 81  AFAHTGISVSITIPNDQIPRLAKPN-FAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANK 140
           AFAHTG+ V I++PNDQ+  +++ N  A  W+  N+  Y PATNI  + VG+EVL++   
Sbjct: 65  AFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTN 124

Query: 141 LLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVIKPM 200
              + LV A++ +  AL  A+LDR+I++STPHS  I+ +S PPS   F + +D  VI P+
Sbjct: 125 -AASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDP-VIVPL 184

Query: 201 LSFLRETNSPLMVNPYPFFACNNEN----LDYALFRP---NPGVVDSDSGILYTNMLDAQ 260
           L FL+ T SPL++N YP+F     N    LDYALF+P   N   VD+++ + YTN+ DA 
Sbjct: 185 LKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAI 244

Query: 261 LDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPL 320
           +DA Y AM  L F ++ IV+ E+GWPSKG P++     + A  YN NL++HV++  GTP 
Sbjct: 245 VDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPK 304

Query: 321 MPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPA 380
            P     TYI+ L+NED +PGPV E+N+GLF  + +PVY + +    A  +         
Sbjct: 305 HPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILAND------- 364

Query: 381 STSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLG-LDCEPIKENGSCYAPNT 440
                        + ++ +C+ K   D + LQ  +D+ CG G +DC  + +  SCY P+ 
Sbjct: 365 -------------TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDD 424

Query: 441 VRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
           V AH+ Y  NAY+Q        CDF     +TT DPS G C +
Sbjct: 425 VVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVF 444

BLAST of ClCG01G000310 vs. ExPASy TrEMBL
Match: A0A1S3AV36 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.7e-239
Identity = 422/482 (87.55%), Postives = 440/482 (91.29%), Query Frame = 0

Query: 1   MAS-PLTPS-HLLLAYTSTLTT-LLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVA 60
           MAS P TPS HLLL Y STL T LLL+L TL+QIQG   GSIGVNYGTVADNLPPPSKVA
Sbjct: 20  MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 79

Query: 61  DFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEP 120
            FLLD+TIIDRVRLFDA+PDILRAFAHTGISVSITIPNDQIPRL KPNFAEEWIKFNI+P
Sbjct: 80  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 139

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILT 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL EASLDRRIQISTPHSLGIL+
Sbjct: 140 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 199

Query: 181 NSSPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVV 240
           NS+PPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFAC  ENLDYALFRPNPGV 
Sbjct: 200 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 259

Query: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYN 300
           DSD GILYTNMLDAQLDAVYSAMKS+ F DLDIVIAETGWPSKGDP+Q GVGPKEAA YN
Sbjct: 260 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 319

Query: 301 GNLMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDL+PVYEIGILR
Sbjct: 320 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 379

Query: 361 PTAQSSTPRAHHGPA----STSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGL 420
           PTAQS+TPRAH GP     ST RSPS S +  SE KRWC+PKT A +EALQRNIDYVCGL
Sbjct: 380 PTAQSATPRAHQGPVEGPISTPRSPSRSTV--SESKRWCVPKTEASVEALQRNIDYVCGL 439

Query: 421 GLDCEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCK 476
           GLDC PI+ENG CYAPNTVR HAAYVMNAYFQATEG + DCDFDQTGALTTVDPSYGKC+
Sbjct: 440 GLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDPSYGKCR 499

BLAST of ClCG01G000310 vs. ExPASy TrEMBL
Match: A0A0A0KJS0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.5e-238
Identity = 417/476 (87.61%), Postives = 432/476 (90.76%), Query Frame = 0

Query: 2   ASPLTPS-HLLLAYTSTLTT-LLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADF 61
           +SP T S HLLL Y STL T LLL+L TL QIQG   GSIGVNYGTVADNLPPPSKVA F
Sbjct: 23  SSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAF 82

Query: 62  LLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYI 121
           LLD+TIIDRVRLFDA+PDILRAFAHTGISVSITIPNDQIPRL KPNFAEEWIKFNI+PYI
Sbjct: 83  LLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYI 142

Query: 122 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNS 181
           PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL EASLDRRIQISTPHSLGIL+NS
Sbjct: 143 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNS 202

Query: 182 SPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDS 241
           +PPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFAC  +NLDYALFRPNPGV DS
Sbjct: 203 TPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDS 262

Query: 242 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGN 301
           D GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPTQ GVGPKEAA YNGN
Sbjct: 263 DLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGN 322

Query: 302 LMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPT 361
           LMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDLSPVYEIGILRPT
Sbjct: 323 LMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPT 382

Query: 362 AQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEP 421
           AQS+TPRAH G         PSP  +SE KRWCLPK+ A  E LQRNIDYVCGLGLDC P
Sbjct: 383 AQSATPRAHQGQVE-----GPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGP 442

Query: 422 IKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           IKENG+C+APNTVRAHAAYVMNAYFQATEGND DCDFDQTG LTTVDPSYGKCKYW
Sbjct: 443 IKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKYW 493

BLAST of ClCG01G000310 vs. ExPASy TrEMBL
Match: A0A6J1H2P3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.6e-237
Identity = 410/476 (86.13%), Postives = 437/476 (91.81%), Query Frame = 0

Query: 1   MASPLTPSHLLLAYTSTLTTLL-LLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADF 60
           MAS    S LL AYTS   TLL LLL TL+Q+Q AK GSIGVNYGT+ADNLP PSKVA+F
Sbjct: 1   MASSFALSRLLRAYTSIAATLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEF 60

Query: 61  LLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYI 120
           LLDSTIIDR+RLFDANPDILRAFAHT ISV+IT+PNDQIPRL KPNFAEEWIKFN++PY+
Sbjct: 61  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 120

Query: 121 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNS 180
           PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL+EASL+RRIQ+STPHSLGIL+NS
Sbjct: 121 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILSNS 180

Query: 181 SPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDS 240
           SPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFF    E LDYALFRPNPGV DS
Sbjct: 181 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 240

Query: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGN 300
           DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDP Q GVGPKEAADYNGN
Sbjct: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 300

Query: 301 LMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSG+GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILRPT
Sbjct: 301 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360

Query: 361 AQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEP 420
           AQSSTPRAHHGP  T + PSPSP+  ++ KRWCLPKT AD EALQRNIDYVCGLGLDC P
Sbjct: 361 AQSSTPRAHHGPEPTPKGPSPSPV--ADGKRWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 421 IKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           I+ENG C+ PNTVRAHAAYVMNAYFQAT+GNDSDCDF QTG LTTVDPSYGKCKYW
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKYW 473

BLAST of ClCG01G000310 vs. ExPASy TrEMBL
Match: A0A6J1K780 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.8e-234
Identity = 404/476 (84.87%), Postives = 433/476 (90.97%), Query Frame = 0

Query: 1   MASPLTPSHLLLAYTS-TLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADF 60
           MAS    S LL AYTS  +T LLLLL TL+Q+Q AK GSIGVNYGT+ADNLP PSKVA+F
Sbjct: 39  MASSFALSRLLRAYTSIAVTLLLLLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEF 98

Query: 61  LLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYI 120
           LLDSTII R+RLFDANPDILRAFAHT ISV+IT+PNDQIPRL KPNFAEEWIKFN++PY+
Sbjct: 99  LLDSTIIGRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 158

Query: 121 PATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNS 180
           PAT+IIR+LVGNEVLSTANK+LIANLVPAMQSLHTAL+EASL+ RIQ+STPHSLGIL+NS
Sbjct: 159 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNSRIQVSTPHSLGILSNS 218

Query: 181 SPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDS 240
           SPPST RFRQGYDTHVIKPMLSFL+ETNSPLMVNPYPFF    E LDYALFRPNPGV DS
Sbjct: 219 SPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 278

Query: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGN 300
           DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDP Q GVGPKEAADYNGN
Sbjct: 279 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 338

Query: 301 LMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSG+GTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDL+PVYEIGILRPT
Sbjct: 339 LLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLNPVYEIGILRPT 398

Query: 361 AQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEP 420
           AQSSTPRAHHGP  T + PSPSP+  ++ KRWCLPKT AD EALQRNIDYVCGLG DC P
Sbjct: 399 AQSSTPRAHHGPEPTPKGPSPSPV--ADGKRWCLPKTEADSEALQRNIDYVCGLGFDCGP 458

Query: 421 IKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKYW 476
           I+ NG C+ PNTVRAHAAYVMNAYFQA +GNDSDCDF QTG LTTVDPSYGKCKYW
Sbjct: 459 IQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDSDCDFQQTGDLTTVDPSYGKCKYW 511

BLAST of ClCG01G000310 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.1e-233
Identity = 414/474 (87.34%), Postives = 432/474 (91.14%), Query Frame = 0

Query: 1   MAS-PLTPS-HLLLAYTSTLTT-LLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVA 60
           MAS P TPS HLLL Y STL T LLL+L TL+QIQG   GSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 61  DFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEP 120
            FLLD+TIIDRVRLFDA+PDILRAFAHTGISVSITIPNDQIPRL KPNFAEEWIKFNI+P
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILT 180
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTAL EASLDRRIQISTPHSLGIL+
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 180

Query: 181 NSSPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVV 240
           NS+PPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFAC  ENLDYALFRPNPGV 
Sbjct: 181 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 240

Query: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYN 300
           DSD GILYTNMLDAQLDAVYSAMKS+ F DLDIVIAETGWPSKGDP+Q GVGPKEAA YN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 300

Query: 301 GNLMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVV+G GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDL+PVYEIGILR
Sbjct: 301 GNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 360

Query: 361 PTAQSSTPRAHHGPA----STSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGL 420
           PTAQS+TPRAH GP     ST RSPS S +  SE KRWC+PKT A +EALQRNIDYVCGL
Sbjct: 361 PTAQSATPRAHQGPVEGPISTPRSPSRSTV--SESKRWCVPKTEASVEALQRNIDYVCGL 420

Query: 421 GLDCEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDP 468
           GLDC PI+ENG CYAPNTVR HAAYVMNAYFQATEG + DCDFDQTGALTTVDP
Sbjct: 421 GLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

BLAST of ClCG01G000310 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 597.8 bits (1540), Expect = 7.5e-171
Identity = 290/458 (63.32%), Postives = 359/458 (78.38%), Query Frame = 0

Query: 17  TLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANP 76
           T+  +LL+LST     G++A SIGVNYGT+A+NLPPP +VA+FLL ST+I+R+RLFD +P
Sbjct: 14  TIVGVLLILST-----GSEA-SIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDP 73

Query: 77  DILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIPATNIIRVLVGNEVLST 136
            IL+AFA+TGI+V++T+PNDQIP L   + A++WI  +I+P+ P+TNIIR+LVGNEV+ST
Sbjct: 74  QILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVIST 133

Query: 137 ANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVI 196
           A+ LLI  L+PAMQSLHTAL  ASL RRIQISTPHSLG LTNS+PPS+ +FR+GYD  V+
Sbjct: 134 ADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVL 193

Query: 197 KPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDSDSGILYTNMLDAQLDAV 256
           KP+LSFLR T+SP +VNPYPFF  + E LD+ALFRPNPG+ D  + +LYTNMLDAQLD+V
Sbjct: 194 KPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSV 253

Query: 257 YSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLMPNR 316
           YSAM  LGF+D++IVI E GWPS+GD  Q GV    AA++N NL+  V SG GTPLMPNR
Sbjct: 254 YSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNR 313

Query: 317 TFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPASTSR 376
           TFETYIFALFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT +SS P   + P     
Sbjct: 314 TFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDP--VYNPR---- 373

Query: 377 SPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNTVRAHA 436
               SP+  S  KRWC+ K GA+  ALQRNIDYVCGLGLDC PI E G CY PNTV+AH+
Sbjct: 374 ----SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHS 433

Query: 437 AYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
            Y MN Y+Q  E ++ DCDFD TG +TT+DPSYG C+Y
Sbjct: 434 KYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of ClCG01G000310 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 585.9 bits (1509), Expect = 3.0e-167
Identity = 286/452 (63.27%), Postives = 354/452 (78.32%), Query Frame = 0

Query: 17  TLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANP 76
           T+  +LL+LST     G++A SIGVNYGT+A+NLPPP +VA+FLL ST+I+R+RLFD +P
Sbjct: 14  TIVGVLLILST-----GSEA-SIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDP 73

Query: 77  DILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIPATNIIRVLVGNEVLST 136
            IL+AFA+TGI+V++T+PNDQIP L   + A++WI  +I+P+ P+TNIIR+LVGNEV+ST
Sbjct: 74  QILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVIST 133

Query: 137 ANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVI 196
           A+ LLI  L+PAMQSLHTAL  ASL RRIQISTPHSLG LTNS+PPS+ +FR+GYD  V+
Sbjct: 134 ADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVL 193

Query: 197 KPMLSFLRETNSPLMVNPYPFFACNNENLDYALFRPNPGVVDSDSGILYTNMLDAQLDAV 256
           KP+LSFLR T+SP +VNPYPFF  + E LD+ALFRPNPG+ D  + +LYTNMLDAQLD+V
Sbjct: 194 KPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSV 253

Query: 257 YSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLMPNR 316
           YSAM  LGF+D++IVI E GWPS+GD  Q GV    AA++N NL+  V SG GTPLMPNR
Sbjct: 254 YSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNR 313

Query: 317 TFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPASTSR 376
           TFETYIFALFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT +SS P   + P     
Sbjct: 314 TFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDP--VYNPR---- 373

Query: 377 SPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNTVRAHA 436
               SP+  S  KRWC+ K GA+  ALQRNIDYVCGLGLDC PI E G CY PNTV+AH+
Sbjct: 374 ----SPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHS 433

Query: 437 AYVMNAYFQATEGNDSDCDFDQTGALTTVDPS 469
            Y MN Y+Q  E ++ DCDFD TG +TT+DPS
Sbjct: 434 KYAMNLYYQTMEKHEFDCDFDNTGEITTIDPS 449

BLAST of ClCG01G000310 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 422.9 bits (1086), Expect = 3.3e-118
Identity = 218/463 (47.08%), Postives = 301/463 (65.01%), Query Frame = 0

Query: 18  LTTLLLLLSTLVQIQGAKAGS-IGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANP 77
           L+ L LLL  L  I  + A S IGVNYG ++DNLPPPS+ A  LL ST I +VRL++A+ 
Sbjct: 3   LSILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAK-LLQSTSIQKVRLYNADS 62

Query: 78  DILRAFAHTGISVSITIPNDQIPRLAKP-NFAEEWIKFNIEPYIPATNIIRVLVGNEVLS 137
            I+ +   TGI + I + N  +P +A   N A +WI  N+ P+ PA+NII + VGNEVL 
Sbjct: 63  SIITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLL 122

Query: 138 TANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHV 197
           + +  L+  L+PAMQ++  AL   SL  +I++ST H++ +L NS PPS G F   Y    
Sbjct: 123 SNDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG- 182

Query: 198 IKPMLSFLRETNSPLMVNPYPFFACNN----ENLDYALFRPNPGVVDSDSGILYTNMLDA 257
           +K +L FL +T SP  +NPYPFFA  +    E L + LF+PNPG VDS++GI Y NM DA
Sbjct: 183 LKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDA 242

Query: 258 QLDAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTP 317
           Q+DAV+SA+KS+GF  +++++AETGWPS GD  + G   + A  YNGNL+ H+ S +GTP
Sbjct: 243 QVDAVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTP 302

Query: 318 LMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGP 377
           LMP ++ +TYIFALF+E+LKPGP  E++FGLF+PDLS  Y+IG+ + T+           
Sbjct: 303 LMPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTS----------- 362

Query: 378 ASTSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNT 437
           + TS+SP    +       WC+PK  A  E LQ ++D+VCG G+DC PI   G C+ PN 
Sbjct: 363 SQTSQSPQLGKV---TSMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPNN 422

Query: 438 VRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
           V +H AY MN YFQ +  N +DCDF +T  +T+ +PSY  C Y
Sbjct: 423 VASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVY 449

BLAST of ClCG01G000310 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 419.1 bits (1076), Expect = 4.8e-117
Identity = 211/441 (47.85%), Postives = 289/441 (65.53%), Query Frame = 0

Query: 39  IGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQI 98
           IGVNYG VADNLPPPS+    LL ST I +VRL+ A+P I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 99  PRLAK-PNFAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALT 158
           P LA  PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 159 EASLDRRIQISTPHSLGILTNSSPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 218
             SL  +I++ST +S+ +L +S PPS+G F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 219 FACNN----ENLDYALFRPNPGVVDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 278
           FA  +    E L + LF PN G VDS +GI YTNM DAQ+DAV+SA+KS+GF  ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 279 ETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLMPNRTFETYIFALFNEDLKPG 338
           ETGW S+GD  + G     A  YNGNL+ H+ S +GTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 339 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPASTSRSPSPSPIPMSEDKRWCL 398
           P  ER FGLF+ DLS VY++G+ + ++ S TP    G  ++S               WC+
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP---SGKVTSS--------------GWCV 385

Query: 399 PKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNTVRAHAAYVMNAYFQATEGNDSD 458
           PK GA  E LQ ++D+ CG G+DC  I+  G+C+ PN V +HAAY MN YFQ +    +D
Sbjct: 386 PKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTD 445

Query: 459 CDFDQTGALTTVDPSYGKCKY 475
           CDF +T  +T+ +PSY  C Y
Sbjct: 446 CDFSKTATVTSQNPSYNNCVY 447

BLAST of ClCG01G000310 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.0e-115
Identity = 215/461 (46.64%), Postives = 302/461 (65.51%), Query Frame = 0

Query: 17  TLTTLLLLLSTLVQIQGAKAGSIGVNYGTVADNLPPPSKVADFLLDSTIIDRVRLFDANP 76
           +++ LLLL + +     A   +IGVNYGT+  NLPPP++VA+F+   T ID V++FD NP
Sbjct: 7   SISLLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVNP 66

Query: 77  DILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIPATNIIRVLVGNEVLST 136
           DILRAFA TGISV +T+PN  IP LA    A  W+  NI P+ P T I  + VGNE+L T
Sbjct: 67  DILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLT 126

Query: 137 ANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILTN--SSPPSTGRFRQGYDTH 196
            +  +I NL+PAM++L+ AL  A + R ++++T HSL I+    +  PS+GRFR G+D  
Sbjct: 127 GDNNMINNLLPAMRNLNNALVRAGV-RDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKG 186

Query: 197 VIKPMLSFLRETNSPLMVNPYPFFACNNENLDYALFR-PNPGVVDSDSGILYTNMLDAQL 256
           ++ P+L++ R T SP MVNPYP+F  + +N+++A+FR P   V D  +  +YTNM DA +
Sbjct: 187 ILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALM 246

Query: 257 DAVYSAMKSLGFADLDIVIAETGWPSKGDPTQFGVGPKEAADYNGNLMRHVVSGMGTPLM 316
           D+ YSAMK+LG+ D++IV+ ETGWPS  D       P  AA +N N+++    G GTPLM
Sbjct: 247 DSTYSAMKALGYGDVNIVVGETGWPSACDAP--WCSPANAAWFNLNIIKR-AQGQGTPLM 306

Query: 317 PNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPAS 376
           PNR FETYIF LFNE+ KPGP  ERN+GLF  D SPVY++G+LR           +G   
Sbjct: 307 PNRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLR-----------NGQGG 366

Query: 377 TSRSPSPSPIPMSEDKRWCLPKTGADLEALQRNIDYVCGLGLDCEPIKENGSCYAPNTVR 436
             R   P+  P +   +WC+ ++GA    LQ +I++VCG G+DC+PI+  GSC+ P+++R
Sbjct: 367 GGRPALPA--PSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLR 426

Query: 437 AHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 475
            HA++VMNAYFQ+    D  C+F  TG +   +PS G CKY
Sbjct: 427 THASFVMNAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906871.12.7e-25591.86glucan endo-1,3-beta-glucosidase [Benincasa hispida][more]
XP_008437641.13.6e-23987.55PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo][more]
XP_011654592.15.1e-23887.61glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus][more]
XP_023521611.16.7e-23886.13glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
XP_023533272.16.7e-23886.13glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P524095.3e-12950.22Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q9M0696.8e-11647.85Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G861.6e-11244.04Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE52.3e-9238.64Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9ZU911.4e-8939.96Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Match NameE-valueIdentityDescription
A0A1S3AV361.7e-23987.55(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV... [more]
A0A0A0KJS02.5e-23887.61(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3... [more]
A0A6J1H2P31.6e-23786.13(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 P... [more]
A0A6J1K7802.8e-23484.87(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=... [more]
A0A5A7TGS64.1e-23387.34(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT5G24318.17.5e-17163.32O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.23.0e-16763.27O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.13.3e-11847.08O-Glycosyl hydrolases family 17 protein [more]
AT4G34480.14.8e-11747.85O-Glycosyl hydrolases family 17 protein [more]
AT3G55430.12.0e-11546.64O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 390..474
e-value: 8.5E-42
score: 154.8
IPR012946X8 domainPFAMPF07983X8coord: 391..460
e-value: 1.2E-19
score: 70.8
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 37..360
e-value: 1.0E-118
score: 397.9
NoneNo IPR availableGENE3D1.20.58.1040coord: 385..474
e-value: 1.3E-10
score: 43.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..382
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..388
NoneNo IPR availablePANTHERPTHR32227:SF62O-GLYCOSYL HYDROLASES FAMILY 17 PROTEINcoord: 28..467
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 39..353
e-value: 4.8E-79
score: 266.2
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 268..281
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 28..467
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 39..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G000310.1ClCG01G000310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds