ClCG01G000210 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG01G000210
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptioncytochrome b5
LocationCG_Chr01: 156146 .. 158349 (-)
RNA-Seq ExpressionClCG01G000210
SyntenyClCG01G000210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGCAATTAACAAGATCAAGAACTCATCTCTAATCGCTCGGGCTTTGCCGCAAACAGGGATCCGAACAATACGTTCCCTCTTCACCTTTTCTCAATCTGTGAGTTAGCTTCTTTCTCACCGACCCATTTCCATTTCTTTTGCTTTTTTCTGCTCTGTCCATGTCTTTTTATTAATTCCCCCCTTTTCAATGCCTCTACACTTGGTTTTGGATCCCTGTTCTTTGTGGGGTTTGTCAATTTCAATTGTTGCAGTGATAAATGGCTTCGGATCCGAAGGTTCATCTATTTGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGCTGGCTTGTAATCTCTGGGAAGGTTGGATCTCTCTTAGTTGATTGTTCTTATCAATTTATTTAACACACAAGATCAGTTCTGGTTTAATTGTTGACTATTTCTTATTTGGTTGGCAACGCATATGGCTAATGTTTTTGGAGAAGGAAAATGAAAAACTCTCGCTAAAGAAAAACATATGGCTGTGGCTAAGGGTTTTAACACTTGCTAACTCTGGATTCTCATGGAATATCCACTAAAAAAGAGTTTGATATAGATGCCTTAGCTTTGGGGTTTCATTTTGTTTTGCATGCTCGTTCTCACATTTCCGTTTACAGATTCTTAAATTCAGATATCTTCTTTCTTTTCCTCATTGTTTTGATTCATGCACAAGATTCTTATGTGTACTTCTAATACAGAAGATAACTTAGTCTCGTTTCGTTCTGTTCCGTTTGTATTATCCGGATTTGCTTGTATGAATGAATCATATGTCACTTATATGTAATGTCTTAATGTCACTGTGCCATAGTCACCTCGTAATGGCGGAGAGCTGTTGTGATGTTTATATATAGTGTATGAAGGCTATATGAAGGACTGACATTGACTAAAGAGTCTTAGAGTTTTGTTTTCCTTCTCCCCATAAGAAATCATAACTTCCATTGGATTGCTTGTTATAATATGCTTAGAAGTATACCCACCCCAAGAGTTGGGCATGGTGGTGAAGACTTGGGGTCAACCAGAGTGGTAATGGGTTTAAATGCCTTTTTTGGCTACCTTGTAAAGATATTGCATTTGTCATATTCTTGGTGAATATTGAGAGCTTTGGGGACCATGCTAAAAGTTCAAAATGCTTATAACTAGAGATGAGGTAATTTAGAAATTAATTGATCGGCTCTCGCTACGATATTTTGAGCCCCAACCACCAGTGGTTGACTCAATAGTAAAAAAAAAAAAAAAAACCACTTAAATTTCCCAGAATCAAATGCCTTAGAGATCATGTGTTCAAAATGAGGTGTTACTAAGCTGGCTTGTTGGGCATCATGAGCAATTTATATTCTGTGGAAGTAGTGAGATATCTTTTCTCATGTTTGATTGCTTGTGACCTTGGCTCTAGGTTTATGATGTAACTCCTTTCATGGAGGATCACCCCGGAGGTGATGAAGTATTATTGTCAGCAACTGGTAATCTCAGAGTTCCACAAACACTGATTCTGTTTCTTTCAGGTCCCTTGCAAATGATGATTAGGATGGATATGATAACAGTTACTCATTGGAATGTTTTGCAGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGATTCAGCAAGAGAAATGATGGACAAATATTACATTGGAGAAATTGATCCTTCAACAGTTCCTTTGAAGAAGATTTACATTCCATCACAACAAACGCAGTATAACCCAGATAAGACACCCGAGTTTGTGATTAAGATCTTGCAGTTTTTGGTTCCCATATTGATTTTGGGTTTGGCGTTTGCTGTTCGACACTACACCAAGAATGAATAAAACCCTCTGGAGGGTAGACTGCTTATTGATATCGATTATGTTTGGTAGAAAGGACGTTCCCTTTTTCTGTTTTAGTTTCTTTTGTTCTGTGTTTTCATGTGGGGGGTTGGTCGTAGACTAGAGTTCTCTGGATCGGATGTTGTATTCTACTTGATAATAAGTAAAGCCAAGGACTATCTTTAGTTTTTTTTTTTCCTTCCTTTTTTCCCTTGTGGCCCTTTAATGATAAACCAACATAGTGAAACTTCATGGTTTTCTATTGTTTTTGTTTCTGATCGAGACAATTGTTCATAGCCGACTTTGGAGTGAACTTTCTCGTTTACATGACACTTATCTATGAAAAAAGTACATCATATCATCCCAAAAC

mRNA sequence

ATAGCAATTAACAAGATCAAGAACTCATCTCTAATCGCTCGGGCTTTGCCGCAAACAGGGATCCGAACAATACGTTCCCTCTTCACCTTTTCTCAATCTTGATAAATGGCTTCGGATCCGAAGGTTCATCTATTTGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGCTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCACCCCGGAGGTGATGAAGTATTATTGTCAGCAACTGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGATTCAGCAAGAGAAATGATGGACAAATATTACATTGGAGAAATTGATCCTTCAACAGTTCCTTTGAAGAAGATTTACATTCCATCACAACAAACGCAGTATAACCCAGATAAGACACCCGAGTTTGTGATTAAGATCTTGCAGTTTTTGGTTCCCATATTGATTTTGGGTTTGGCGTTTGCTGTTCGACACTACACCAAGAATGAATAAAACCCTCTGGAGGGTAGACTGCTTATTGATATCGATTATGTTTGGTAGAAAGGACGTTCCCTTTTTCTGTTTTAGTTTCTTTTGTTCTGTGTTTTCATGTGGGGGGTTGGTCGTAGACTAGAGTTCTCTGGATCGGATGTTGTATTCTACTTGATAATAAGTAAAGCCAAGGACTATCTTTAGTTTTTTTTTTTCCTTCCTTTTTTCCCTTGTGGCCCTTTAATGATAAACCAACATAGTGAAACTTCATGGTTTTCTATTGTTTTTGTTTCTGATCGAGACAATTGTTCATAGCCGACTTTGGAGTGAACTTTCTCGTTTACATGACACTTATCTATGAAAAAAGTACATCATATCATCCCAAAAC

Coding sequence (CDS)

ATGGCTTCGGATCCGAAGGTTCATCTATTTGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGCTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCACCCCGGAGGTGATGAAGTATTATTGTCAGCAACTGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGATTCAGCAAGAGAAATGATGGACAAATATTACATTGGAGAAATTGATCCTTCAACAGTTCCTTTGAAGAAGATTTACATTCCATCACAACAAACGCAGTATAACCCAGATAAGACACCCGAGTTTGTGATTAAGATCTTGCAGTTTTTGGTTCCCATATTGATTTTGGGTTTGGCGTTTGCTGTTCGACACTACACCAAGAATGAATAA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE
Homology
BLAST of ClCG01G000210 vs. NCBI nr
Match: XP_011654591.1 (cytochrome b5 [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_000069 [Cucumis sativus])

HSP 1 Score: 275.4 bits (703), Expect = 2.6e-70
Identity = 131/134 (97.76%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKI+IPSQQ+Q+NPDKTPEFVIKILQFLVPILI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. NCBI nr
Match: XP_038875650.1 (cytochrome b5 [Benincasa hispida])

HSP 1 Score: 275.0 bits (702), Expect = 3.4e-70
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AREMMDKYYIGEIDPSTVP KKIYIPSQQTQ+NPDKTPEFVIKILQFLVPILI
Sbjct: 61  DVGHSDAAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. NCBI nr
Match: XP_008437642.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 274.2 bits (700), Expect = 5.8e-70
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKV LFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVRLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQ+NPDKTPEFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. NCBI nr
Match: XP_022922141.1 (cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_023549751.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG6579306.1 Cytochrome b5 isoform E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016804.1 Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271.2 bits (692), Expect = 4.9e-69
Identity = 129/134 (96.27%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KIYIPSQQTQYNPDKT EFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. NCBI nr
Match: XP_022137808.1 (cytochrome b5 [Momordica charantia])

HSP 1 Score: 270.0 bits (689), Expect = 1.1e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLK++YIP+QQTQYN DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 3.7e-59
Identity = 106/134 (79.10%), Postives = 119/134 (88.81%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKTPEF+IKILQFLVPILI
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

BLAST of ClCG01G000210 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 2.6e-57
Identity = 105/134 (78.36%), Postives = 117/134 (87.31%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDATNDFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MM+KYYIGEID STVP  + Y+   Q  YN DKTPEF+IKILQFLVPILI
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VR YTK E
Sbjct: 121 LGLALVVRQYTKKE 134

BLAST of ClCG01G000210 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 198.7 bits (504), Expect = 4.0e-50
Identity = 89/129 (68.99%), Postives = 109/129 (84.50%), Query Frame = 0

Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHS 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLLS+TGKDAT+DFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  DSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAF 125
            +AR MMD+YY+G+ID ST+P +  Y+P +Q  YN DKTPEF+IKILQFLVP+ ILGLA 
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 AVRHYTKNE 135
           A+R YTK+E
Sbjct: 127 AIRIYTKSE 135

BLAST of ClCG01G000210 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 6.9e-50
Identity = 89/132 (67.42%), Postives = 107/132 (81.06%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLLSATGKDAT+DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHS SAR M+D+YY+G+ID +T+P K  Y P  Q  YN DKT EFV+K+LQFLVP++I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFAVRHYTK 133
           LG+AF +R YTK
Sbjct: 121 LGVAFGIRFYTK 132

BLAST of ClCG01G000210 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 5.8e-49
Identity = 86/133 (64.66%), Postives = 110/133 (82.71%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  Y P +Q  YN DKT EF+IK+LQFLVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

BLAST of ClCG01G000210 vs. ExPASy TrEMBL
Match: A0A0A0KN46 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-70
Identity = 131/134 (97.76%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKI+IPSQQ+Q+NPDKTPEFVIKILQFLVPILI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. ExPASy TrEMBL
Match: A0A1S3AU68 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103482987 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.8e-70
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKV LFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVRLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQ+NPDKTPEFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. ExPASy TrEMBL
Match: A0A6J1E3B3 (cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-69
Identity = 129/134 (96.27%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KIYIPSQQTQYNPDKT EFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. ExPASy TrEMBL
Match: A0A6J1CBE4 (cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 5.3e-69
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLK++YIP+QQTQYN DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. ExPASy TrEMBL
Match: A0A6J1K8E3 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111491071 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 6.9e-69
Identity = 129/134 (96.27%), Postives = 130/134 (97.01%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP KKIYIPSQQTQYN DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

BLAST of ClCG01G000210 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 228.8 bits (582), Expect = 2.6e-60
Identity = 106/134 (79.10%), Postives = 119/134 (88.81%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKTPEF+IKILQFLVPILI
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

BLAST of ClCG01G000210 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 194.9 bits (494), Expect = 4.2e-50
Identity = 86/133 (64.66%), Postives = 110/133 (82.71%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQFLVPILI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  Y P +Q  YN DKT EF+IK+LQFLVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

BLAST of ClCG01G000210 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 182.6 bits (462), Expect = 2.1e-46
Identity = 81/136 (59.56%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L++TGKDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQ--YNPDKTPEFVIKILQFLVPI 120
           DVGHS +A+ M+D+YY+G+ID +TVP+K  ++P   T+     DK+ +FVIK+LQFLVP+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LILGLAFAVRHYTKNE 135
           LILGLAF +R+YTK +
Sbjct: 121 LILGLAFGIRYYTKTK 136

BLAST of ClCG01G000210 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 139.8 bits (351), Expect = 1.6e-33
Identity = 66/123 (53.66%), Postives = 90/123 (73.17%), Query Frame = 0

Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAR 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLL+ TGKDA+ DFEDV HS  A+
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKT--PEFVIKILQFLVPILILGLAFAV 129
           E+M KY IG++D STVP+ + YIP  + +    +T   E   K+L +L+P+LILG+AFA+
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 130 RHY 131
           R Y
Sbjct: 127 RFY 129

BLAST of ClCG01G000210 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 125.6 bits (314), Expect = 3.1e-29
Identity = 62/127 (48.82%), Postives = 88/127 (69.29%), Query Frame = 0

Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHS 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLL+  GKDAT+DFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQYNPDKTPEFVIKILQ-----FLVPILI 125
             ARE+M+KY+IGE+D S++P     IP  +  Y  D+  + V K+       ++VP+ I
Sbjct: 66  KDARELMEKYFIGELDESSLP----EIPELKI-YKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAFAV 128
           + ++ AV
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011654591.12.6e-7097.76cytochrome b5 [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_000069 [Cuc... [more]
XP_038875650.13.4e-7097.76cytochrome b5 [Benincasa hispida][more]
XP_008437642.15.8e-7097.76PREDICTED: cytochrome b5 [Cucumis melo][more]
XP_022922141.14.9e-6996.27cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5... [more]
XP_022137808.11.1e-6895.52cytochrome b5 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q423423.7e-5979.10Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2[more]
P409342.6e-5778.36Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1[more]
P491004.0e-5068.99Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2[more]
P490986.9e-5067.42Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O488455.8e-4964.66Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KN461.3e-7097.76Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
A0A1S3AU682.8e-7097.76cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103482987 PE=3 SV=1[more]
A0A6J1E3B32.4e-6996.27cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3... [more]
A0A6J1CBE45.3e-6995.52cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1[more]
A0A6J1K8E36.9e-6996.27cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111491071 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G53560.12.6e-6079.10cytochrome B5 isoform E [more]
AT2G32720.14.2e-5064.66cytochrome B5 isoform B [more]
AT5G48810.12.1e-4659.56cytochrome B5 isoform D [more]
AT2G46650.11.6e-3353.66cytochrome B5 isoform C [more]
AT1G26340.13.1e-2948.82cytochrome B5 isoform A [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 30..40
score: 60.74
coord: 40..54
score: 54.65
coord: 55..62
score: 45.27
coord: 68..80
score: 44.99
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..81
e-value: 1.1E-30
score: 117.9
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 11..80
e-value: 3.0E-27
score: 94.5
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 5..81
score: 29.527899
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 1..86
e-value: 1.2E-36
score: 126.5
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 4..87
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..132
NoneNo IPR availablePANTHERPTHR19359:SF103CYTOCHROME B5-LIKEcoord: 1..132
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G000210.1ClCG01G000210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035672 oligopeptide transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0035673 oligopeptide transmembrane transporter activity