Chy9G170470 (gene) Cucumber (hystrix) v1

Overview
NameChy9G170470
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationchrH09: 15163118 .. 15167188 (+)
RNA-Seq ExpressionChy9G170470
SyntenyChy9G170470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAATTCAAGATGATGGCATGATCAGGATTCTTAATAATAATCACAGCCGTTCCTCACCCATCTCCAATATTCCGATTCCTTCTCATTCCCCACCAATGGCGAGAGCCTTCTTAGGTTCCACGCTTTCCTCTTCCCCACTAATCGCTTTACGCCTCTCTTCCTTTAACCCATCATTTTTTTTAACCAATCAACGCCCTCGCTCCCTTCGTCTCACCACCGACACTGTCGTCGCTATGGCGGAGCTTCCGTCGGAGAAGCGCATTAAGCCCCACCCATCTCTCGAGGTCATCGGTGGAGGCTGCGATCAGTTCCTCCCAGCTTTTAAGGACTTGGATCTCCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTGGAGACCATTTTTGCTTCCTTTTTTAGGACTTGTCCCAGTGTTAACCTCCACCGGGAGTGTCTTCGTGCGGCTGATAATGGAACTGTTGCGCTCGATTGGGTCGCCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGGTATGTGCTTAATGATTATTGGTTTTTTGAAGAATTTATGAATAAGATAGAAATGGCAAAGATATTCCTAAACCAAAATTATATGAATCGATTTGGGCATCCTTGAAAAGAAACGATTTTACATACCCAATTCGTTAATCCATTTAATCCTACTTGTATTTGTAATTACTCTATGGTCTATAGCTCTCGTGTTGCTCTTTTTTCCTTTTTTTTTTTGTTTCCCTTCTCCCATTGAGGGGCTTTAATTTTTCCCATCTTTAGTAAAACCTCCTAAGGAAGTGCAAGTTCTTTGCTTGGAAACCTTTACATGGTAGAATGTGGCATTCTTCTTAAATTCTATGCCCAAAGTGTGCCCTGTTTGCCACCTACCTAGTATTTAATATCCTATGGATTTCTTGACACCCTTTCTTGACACCCCATTGTTGTAGAGACAATTATCTTGTGAGGTTAGTCCAGGTGCACATCTAACTTGAACACACGGTTAAAAAAAAGAAGGTAGTGTGTCCTCTGTGTCCTCTATAGTGGGGAAGACTCTACAAACTCTCCCTTTCCTGGTAAAGGAAGCGTAGGAGGTTGTGAGGTTTAATCGTCTTTATGAGTGTGTGGATAGATCATTGTGTAATTGTCAGCTTGTAGTAATTAGTTTGGAGGTTAGGTGCGGTTTATGGAGAAATGAGAATCAGTCTACAGTACTGATTGTGGGAGAGAAATATTTCTCTTGAAAGGCTATTGATACTGTAATTTTACCTTTGATATTGCGATATAGTTTTACTGTCGACTTCTTGTAATTGGGATCATTATAGTTCCTCGATTCATATCAAAACATTTAATTTTTTTTTCTTGAACTTCTGCAGCCTGGTTTAACAGGAGGGAGTCAGGATTCATATGTAAGACACATGTTGGTTAGAGCTAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAATAGCCGTGGATGTGGGAATAGCCCTGTTACCACTCCTCAGGTATCCATTTTTTGTTCTTCTTTATGTTGTATTGAGTATCATATTGCTTTGACATCAGATGATTTTTTTCTTTCTCAATATTCGGAATTGACATTCTTGTTTATGATTCACTTTACAACACTGATATATGCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCATGTTACAGAAAGATATCCCAAGGCTAATTTGTATGCTGTTGGCTGGTCACTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGTACAAGTAAAACAATACATATTAATCTCCTTCACTCTTTACTCTACTTATTGGTTGCCATTTTGTTTAAACCTCCTGAGTCTCATGGAACGACGTAAATTGGGGTATTTAATTCAAATTTGCGAGAAAAAGTCATTTGGAATAATGTTTGTCTTGACGAGACAACGAAGCAAAAATTTAAGTTCGTGGGCTAATGTTTGAAATGAACAATATTATAACATTTTGGGGATTCAGCGTAACAGAAGTTCAGCAACACATAAGACACTTCAAGATTTGTGTGAATAAACTTGAAACAAAGAGAGCTATTCTTTTAAAGATTACATTGTTGAATGACATATTTTGTGATAACGCAAAGTTCAATGTCTGAGAGTTCGGTGTTCTGGGGACTCTTTGTTTTTGTTTGTTTGTTTTTTTTTTTTTTTTCTAATTCATCAAGTGGGGTATGGGAATTCAAACCGACCTCCAGGGAGGTAATACATGTTAATTACCCTCTTATGCCAAAATCTCTCAGTACAATGTTTAAGTCGCTTTTCCATATCATATTTATATCTATATATCCATCTTTATCTCTTACATATCACTCCTACATCTTCATTGCAGGATTCCCTGACCTGTCCATTGTCTGGTGCTGTGTCTTTATGTAATCCATTCGATCTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAACGTCTATGATAAATCCCTGGCTGGAGCGTTACGTAAAATTTTCAACAAGTAATTCTCTTATCTGAATACGTTTTATTTTGTTAATTGAGTCATAACCTGAAACTGAAACATTCGTCGAGCCTTAGATTGTAAAATGTATAATCTCACTAGACTCCCATCGTAGGCATGCTGCTCTCTTCACTGATATGCCTAGTGAATTCAATATTCGCTTGGCTGCCAAAGCTACAACTGTTAGAGACTATGATGATGCCTTAACACGCGGTATACATTCTCTTAGTTTTTGTTATTTTCATTGGCAGAACATTATCATATATTCCATTTCTTCTCCTTTAAACTTTTATTTTACCTCTATCTTATTATTATTTTTTTCTTTGAACAACTTTCAGTTTCATTTGGTTTCAACTCTGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAATTCATAAAAGATGTCAAAATTCCTTTGCTTTGCATCCAGGTACCATTTTTGTTTTTTCAAATGTTGTCTTGTTTAATTGTTCTTTAAGAACTCAAAATTGCTGTCAAATCTCTTTTAAGTTATCATCTGTAAGGAAAATAATATATTGTAATTACTCTTTTCAAAACAGTTTCCAAACAACTATTATAGTCTCTCTTTTCCTTTAACTTCTTGTTAATTGATTTGGGGAACTTACAGAATGTCTAGGGAAATTGTAGCTAAGAGCACTGTTCTCTCACAGATATTTTTGGGCATTCTATACAATATTGTTCTTGTAATGTTAAGGGGCATTTAATTTGGTTTTCAGGCAGCAAATGATCCAATTGCTCCAAATAGAGGAATCCCCCGAGAAGATATAGAGGTTCCACACATGTTTATCTTCAAGTTTTCTAAGTTATCTATTAAAAATCTATTGAAAAGTTTAATGTCATTTCATAAGCATTTACTTTTTTAGAGGCCAAGTTTTAAAAACTAAGGCCCGTTTTATTTTTTGTTTTTGAAAATCATGCTTATAAGCAATATGTGTGCCATAAGTTCTTGGTTTATTCTTCCTTTAACAGCCGTTTCATTGTTAAGGTTGTTGAAATTTGTGTATTTTTTCACACAATTTCTTCTAATAAGTTTCATTTTTTCCAAAAAAAAATCAGAGTTCTCAACCAAATTTTGAAAACAAAAATATGTTTCCAAAAATTATCTTCTTGTTTGTTTCCGAAACCTTGCTCTAATTTTAAAAATATTGTTAAAAGTTAGATGGAAAAACATAAAAACCTTTACGTGGAACGAGTGTTTTACCATTGCTTTTGTTTTTAGAGTTTGATTAAAGATTTAAATATGATTCTACGTACACCTCTATTGTTTAACATGCACCCTTAGCTTCTTGTGATTGAGCATATTTTTGAAACGTGATTGTTGTTGCAGGAAAATCCAAACTGTATGTTAATAGTAACACCCAAAGGTGGGCATCTAGGGTGGGTTGCAGGGCCCGGTGCTCCCCTCGGTTTTCCATGGACCGATCCATTCGTGATCGATTTCCTCATGTATTTGGAAAACCAAAAATTCAAAGCCTCAGCTTCTTCTACAGGTTTAACAACAGCAGAGGTAATGACATAG

mRNA sequence

ATGCAAATTCAAGATGATGGCATGATCAGGATTCTTAATAATAATCACAGCCGTTCCTCACCCATCTCCAATATTCCGATTCCTTCTCATTCCCCACCAATGGCGAGAGCCTTCTTAGGTTCCACGCTTTCCTCTTCCCCACTAATCGCTTTACGCCTCTCTTCCTTTAACCCATCATTTTTTTTAACCAATCAACGCCCTCGCTCCCTTCGTCTCACCACCGACACTGTCGTCGCTATGGCGGAGCTTCCGTCGGAGAAGCGCATTAAGCCCCACCCATCTCTCGAGGTCATCGGTGGAGGCTGCGATCAGTTCCTCCCAGCTTTTAAGGACTTGGATCTCCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTGGAGACCATTTTTGCTTCCTTTTTTAGGACTTGTCCCAGTGTTAACCTCCACCGGGAGTGTCTTCGTGCGGCTGATAATGGAACTGTTGCGCTCGATTGGGTCGCCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACAGGAGGGAGTCAGGATTCATATGTAAGACACATGTTGGTTAGAGCTAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAATAGCCGTGGATGTGGGAATAGCCCTGTTACCACTCCTCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCATGTTACAGAAAGATATCCCAAGGCTAATTTGTATGCTGTTGGCTGGTCACTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGATTCCCTGACCTGTCCATTGTCTGGTGCTGTGTCTTTATGTAATCCATTCGATCTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAACGTCTATGATAAATCCCTGGCTGGAGCGTTACGTAAAATTTTCAACAAGCATGCTGCTCTCTTCACTGATATGCCTAGTGAATTCAATATTCGCTTGGCTGCCAAAGCTACAACTGTTAGAGACTATGATGATGCCTTAACACGCGTTTCATTTGGTTTCAACTCTGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAATTCATAAAAGATGTCAAAATTCCTTTGCTTTGCATCCAGGCAGCAAATGATCCAATTGCTCCAAATAGAGGAATCCCCCGAGAAGATATAGAGGAAAATCCAAACTGTATGTTAATAGTAACACCCAAAGGTGGGCATCTAGGGTGGGTTGCAGGGCCCGGTGCTCCCCTCGGTTTTCCATGGACCGATCCATTCGTGATCGATTTCCTCATGTATTTGGAAAACCAAAAATTCAAAGCCTCAGCTTCTTCTACAGGTTTAACAACAGCAGAGGTAATGACATAG

Coding sequence (CDS)

ATGCAAATTCAAGATGATGGCATGATCAGGATTCTTAATAATAATCACAGCCGTTCCTCACCCATCTCCAATATTCCGATTCCTTCTCATTCCCCACCAATGGCGAGAGCCTTCTTAGGTTCCACGCTTTCCTCTTCCCCACTAATCGCTTTACGCCTCTCTTCCTTTAACCCATCATTTTTTTTAACCAATCAACGCCCTCGCTCCCTTCGTCTCACCACCGACACTGTCGTCGCTATGGCGGAGCTTCCGTCGGAGAAGCGCATTAAGCCCCACCCATCTCTCGAGGTCATCGGTGGAGGCTGCGATCAGTTCCTCCCAGCTTTTAAGGACTTGGATCTCCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTGGAGACCATTTTTGCTTCCTTTTTTAGGACTTGTCCCAGTGTTAACCTCCACCGGGAGTGTCTTCGTGCGGCTGATAATGGAACTGTTGCGCTCGATTGGGTCGCCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACAGGAGGGAGTCAGGATTCATATGTAAGACACATGTTGGTTAGAGCTAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAATAGCCGTGGATGTGGGAATAGCCCTGTTACCACTCCTCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCATGTTACAGAAAGATATCCCAAGGCTAATTTGTATGCTGTTGGCTGGTCACTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGATTCCCTGACCTGTCCATTGTCTGGTGCTGTGTCTTTATGTAATCCATTCGATCTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAACGTCTATGATAAATCCCTGGCTGGAGCGTTACGTAAAATTTTCAACAAGCATGCTGCTCTCTTCACTGATATGCCTAGTGAATTCAATATTCGCTTGGCTGCCAAAGCTACAACTGTTAGAGACTATGATGATGCCTTAACACGCGTTTCATTTGGTTTCAACTCTGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAATTCATAAAAGATGTCAAAATTCCTTTGCTTTGCATCCAGGCAGCAAATGATCCAATTGCTCCAAATAGAGGAATCCCCCGAGAAGATATAGAGGAAAATCCAAACTGTATGTTAATAGTAACACCCAAAGGTGGGCATCTAGGGTGGGTTGCAGGGCCCGGTGCTCCCCTCGGTTTTCCATGGACCGATCCATTCGTGATCGATTTCCTCATGTATTTGGAAAACCAAAAATTCAAAGCCTCAGCTTCTTCTACAGGTTTAACAACAGCAGAGGTAATGACATAG

Protein sequence

MQIQDDGMIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRPRSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKFKASASSTGLTTAEVMT*
Homology
BLAST of Chy9G170470 vs. ExPASy Swiss-Prot
Match: Q40863 (Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.3e-127
Identity = 234/405 (57.78%), Postives = 289/405 (71.36%), Query Frame = 0

Query: 49  IALRLSSFNPSFFLTNQRPRSLRL-TTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLP 108
           I+ R+S F   F   +++P +      D + + +    ++R K  P  E+IG    + + 
Sbjct: 25  ISRRISQFRAGF--PDKKPAAGACEEQDELTSGSAARIQRRDKQLP--EIIGSD-RELMS 84

Query: 109 AFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDW-VAGDD 168
               L  PY+ FP +G NRH+ETIFASFFR+ P +   RECLR  D GTV LDW + G+D
Sbjct: 85  KLTTLGRPYRHFPFMG-NRHVETIFASFFRSWPVIKSRRECLRMEDGGTVELDWPLEGED 144

Query: 169 L-----RLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTP 228
                  LP++SPVLILLPGLTGGS DSYV+HML+RAR  GW  VVFNSRGC +SPVTTP
Sbjct: 145 AELWNGELPVNSPVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFNSRGCADSPVTTP 204

Query: 229 QFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCN 288
           QFYSASF  D+ +VV HV  R+ ++N+YAVGWSLG NILV YLG+ +  CPLSGAVSLCN
Sbjct: 205 QFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAGNCPLSGAVSLCN 264

Query: 289 PFDLIVADEDFRK--GFNNVYDKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRD 348
           PF+L++ADEDF K  GFNNVYDK+LA  LR+IF KH  LF  +  E+NI   AKA +VRD
Sbjct: 265 PFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYNIPTVAKARSVRD 324

Query: 349 YDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENP 408
           +D  LTRVSFGF SV +YYS SSSS  IK V+  LLCIQA+NDPIAP+RGIP EDI+ENP
Sbjct: 325 FDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPSRGIPWEDIKENP 384

Query: 409 NCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKFK 445
           NC+L+VTP GGHLGWVAG  AP G PWTDP V+++L  LE  + +
Sbjct: 385 NCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLEKNQIE 423

BLAST of Chy9G170470 vs. ExPASy Swiss-Prot
Match: Q0VC00 (Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.5e-51
Identity = 100/293 (34.13%), Postives = 166/293 (56.66%), Query Frame = 0

Query: 129 ETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDS---PVLILLPGLTGG 188
           +T+   F  + P V    E ++ AD G ++LDW   D+ +  +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPLVQYRNELIKTADGGQISLDWFDNDNSKHYMDASTRPTVLLLPGLTGT 151

Query: 189 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPK 248
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  S   D+  V+ HV   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRYVVFNNRGVAGENLLTPRTYCCSNTEDLETVIHHVHSLYPS 211

Query: 249 ANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKSL 308
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGPKTPLKAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 309 AGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSS 368
              L+   NKH  +F     + ++    KA ++R++D   T V FG+ ++D+YY+ +S +
Sbjct: 272 TTCLQSSVNKHRHMFV---KQIDVDHVMKAKSIREFDKRFTSVMFGYRTIDDYYTDASPN 331

Query: 369 KFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAG 418
           + +K V IP+LC+ + +D  +P+  IP E  ++NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Chy9G170470 vs. ExPASy Swiss-Prot
Match: Q8WU67 (Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2)

HSP 1 Score: 203.4 bits (516), Expect = 5.6e-51
Identity = 100/293 (34.13%), Postives = 164/293 (55.97%), Query Frame = 0

Query: 129 ETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDS---PVLILLPGLTGG 188
           +T+   F  + P V    E ++ AD G ++LDW   D+    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNDNSTCYMDASTRPTILLLPGLTGT 151

Query: 189 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPK 248
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  V+ HV   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYPS 211

Query: 249 ANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKSL 308
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 309 AGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSS 368
              L+   NKH  +F     + ++    KA ++R++D   T V FG+ ++D+YY+ +S S
Sbjct: 272 TTCLQSSVNKHRHMFV---KQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPS 331

Query: 369 KFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAG 418
             +K V IP+LC+ + +D  +P+  IP E  ++NPN  L++T  GGH+G++ G
Sbjct: 332 PRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Chy9G170470 vs. ExPASy Swiss-Prot
Match: Q91ZH7 (Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 8.1e-50
Identity = 98/293 (33.45%), Postives = 165/293 (56.31%), Query Frame = 0

Query: 129 ETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDS---PVLILLPGLTGG 188
           +T+   F  + P V    E ++ AD G ++LDW   ++    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNNNSAYYVDASTRPTILLLPGLTGT 151

Query: 189 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPK 248
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  VV HV   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYPG 211

Query: 249 ANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKSL 308
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   +  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYL 271

Query: 309 AGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSS 368
              L+    KH  +F +   + ++    KA ++R++D   T V FG+ ++D+YY+ +S +
Sbjct: 272 TTCLQSSVKKHRHMFVE---QIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPN 331

Query: 369 KFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAG 418
           + +K V IP+LC+ A +D  +P+  IP E  ++NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNATDDVFSPSHAIPIETAKQNPNVALVLTAYGGHIGFLEG 381

BLAST of Chy9G170470 vs. ExPASy Swiss-Prot
Match: Q54H38 (Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=abhd PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.2e-46
Identity = 113/337 (33.53%), Postives = 182/337 (54.01%), Query Frame = 0

Query: 107 PAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNL--HRECL-RAADNGTVALDWVA 166
           P  K+    Y P+ +   N H    + S+    P +NL   RE L    D GT++LD+  
Sbjct: 53  PTIKNGVTFYPPYYLY--NSHFMNYYGSY--KIPKLNLKTRREILVNPIDGGTISLDFFE 112

Query: 167 GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRA-RDRGWRVVVFNSRGCGNSPVTTPQ 226
             + +   D+P +++  GLTGGS + YV++   RA +++G+R VVFN RGC  +P+T  +
Sbjct: 113 LGEFK--EDTPTIVINHGLTGGSHERYVQYFAQRAYKEKGFRSVVFNYRGCAGNPITADR 172

Query: 227 FYSASFLGDMREVVAHVTERYPK--ANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLC 286
            YSA  L D++ V  ++T+         + VG+SLG  ILVNY+       P    VS+ 
Sbjct: 173 AYSAVQLDDIKFVTEYLTKTALPLVKKWFLVGFSLGSAILVNYMADAGKDSPYLAHVSIS 232

Query: 287 NPFDLIVADEDFRKGF--NNVYDKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVR 346
           NP +++   ++    +  N +Y+K LA  L+++F K         ++  I     A T+ 
Sbjct: 233 NPMNMVECTKNLSSTYINNLIYNKGLANNLKRLFRKFDGRLDKYATKEQI---MAAQTIA 292

Query: 347 DYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEEN 406
           D+DD +T   FGF +  +YY  +SSSK I+++  P+L I A +DPIAP  G P +D + N
Sbjct: 293 DFDDLITSKMFGFETAHDYYLAASSSKSIRNLVKPILFINAIDDPIAPTSGFPWKDFKSN 352

Query: 407 PNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFL 436
           PN +L V+  GGHLG+++         W+D   +++L
Sbjct: 353 PNTILCVSRWGGHLGFISYED---HMSWSDKAAVEYL 377

BLAST of Chy9G170470 vs. ExPASy TrEMBL
Match: A0A0A0KNN2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148530 PE=4 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.2e-263
Identity = 447/459 (97.39%), Postives = 452/459 (98.47%), Query Frame = 0

Query: 1   MQIQDDGMIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSF 60
           +QIQDDG+IRILNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALRLSSF PSF
Sbjct: 22  IQIQDDGIIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTPSSSPLIALRLSSFTPSF 81

Query: 61  FLTNQRPRSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLRLTTDTVV MAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 82  FLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 141

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP
Sbjct: 142 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 201

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 202 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 261

Query: 241 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 262 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 321

Query: 301 DKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSK 360
           DKSLAGALRKIFNKHAALFTDM  EFNIRLAA+ATTVRDYDDALTRVSFGFN+VDEYYSK
Sbjct: 322 DKSLAGALRKIFNKHAALFTDMTGEFNIRLAAQATTVRDYDDALTRVSFGFNTVDEYYSK 381

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA
Sbjct: 382 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 441

Query: 421 PLGFPWTDPFVIDFLMYLENQKFKASASSTGLTTAEVMT 460
           PLGFPWTDPFVIDFLMYLEN+KFKASASST LT AEVMT
Sbjct: 442 PLGFPWTDPFVIDFLMYLENKKFKASASSTDLTMAEVMT 480

BLAST of Chy9G170470 vs. ExPASy TrEMBL
Match: A0A5A7TMR0 (Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001510 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.6e-258
Identity = 438/459 (95.42%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 1   MQIQDDGMIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSF 60
           MQIQDD M   LNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALR+SSF PSF
Sbjct: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60

Query: 61  FLTNQRPRSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLR TT +VVAMAE+PSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWV GDDLRLPLDSPVLILLP
Sbjct: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240

Query: 241 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           +RYPKAN+YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300

Query: 301 DKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSK 360
           DKSLA ALRKIFNKHAALFTDMPSEFNIRLAAKA TVRDYDDALTRVSFGFN+VDEYYSK
Sbjct: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDI+ENPNCMLIVTP+GGHLGWVAGPGA
Sbjct: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420

Query: 421 PLGFPWTDPFVIDFLMYLENQKFKASASSTGLTTAEVMT 460
           PLGFPWTDPFVIDFLMYLENQK KASASST LTTAEVMT
Sbjct: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 459

BLAST of Chy9G170470 vs. ExPASy TrEMBL
Match: A0A1S3AU57 (LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=3656 GN=LOC103482859 PE=4 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 432/452 (95.58%), Postives = 440/452 (97.35%), Query Frame = 0

Query: 8   MIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRP 67
           M   LNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALR+SSF PSFFLTNQRP
Sbjct: 1   MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP 60

Query: 68  RSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 127
           RSLR TT +VVAMAE+PSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH
Sbjct: 61  RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 120

Query: 128 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQ 187
           LETIFASFFRTCPSVNLHRECLRAADNGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ 180

Query: 188 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKAN 247
           DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT+RYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN 240

Query: 248 LYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGA 307
           +YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLA A
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA 300

Query: 308 LRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFI 367
           LRKIFNKHAALFTDMPSEFNIRLAAKA TVRDYDDALTRVSFGFN+VDEYYSKSSSSKFI
Sbjct: 301 LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI 360

Query: 368 KDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWT 427
           KDVKIPLLCIQAANDPIAPNRGIPREDI+ENPNCMLIVTP+GGHLGWVAGPGAPLGFPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT 420

Query: 428 DPFVIDFLMYLENQKFKASASSTGLTTAEVMT 460
           DPFVIDFLMYLENQK KASASST LTTAEVMT
Sbjct: 421 DPFVIDFLMYLENQKSKASASSTDLTTAEVMT 452

BLAST of Chy9G170470 vs. ExPASy TrEMBL
Match: A0A6J1GD89 (embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC111453123 PE=4 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 8.1e-218
Identity = 370/433 (85.45%), Postives = 395/433 (91.22%), Query Frame = 0

Query: 26  PIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRPRSLRLTTDTVVAMAELPS 85
           PIP H PPMARAFLGST SSS L   RLSSF PS  + NQR RSLRLTT  VVAM+E PS
Sbjct: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LINNQRRRSLRLTTAAVVAMSEFPS 79

Query: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145
           EK I+PHPSLEVIGGGCD+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 80  EKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139

Query: 146 RECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199

Query: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLG 265
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT+RYPKAN+YAVGWSLGGNILVNYLG
Sbjct: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259

Query: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMPSE 325
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LA ALR+IF+KHA LF DMP E
Sbjct: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319

Query: 326 FNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385
           FNI LAA A TVR+YDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379

Query: 386 PNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKFKA 445
           P+RG+PREDI+ENPNC+LIVTPKGGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+ KA
Sbjct: 380 PDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439

Query: 446 SASSTGLTTAEVM 459
           SASST LT AE +
Sbjct: 440 SASSTDLTAAETL 451

BLAST of Chy9G170470 vs. ExPASy TrEMBL
Match: A0A6J1IJD9 (embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111477973 PE=4 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.6e-216
Identity = 369/433 (85.22%), Postives = 394/433 (90.99%), Query Frame = 0

Query: 26  PIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRPRSLRLTTDTVVAMAELPS 85
           PIP H PPMARAFLGST SSS L   RLSSF PS  +TNQR RSLRLTT  VVAM+E PS
Sbjct: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LITNQRRRSLRLTTAAVVAMSEFPS 79

Query: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145
           EK I+PHPSLEVIGGG D+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 80  EKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139

Query: 146 RECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199

Query: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLG 265
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT+RYPKAN+YAVGWSLGGNILVNYLG
Sbjct: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259

Query: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMPSE 325
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LA ALR+IF+KHA LF DMP E
Sbjct: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319

Query: 326 FNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385
           FNI LAA A TVR+YDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379

Query: 386 PNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKFKA 445
           P+RG+PREDI+ NPNC+LIVTPKGGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+ KA
Sbjct: 380 PDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439

Query: 446 SASSTGLTTAEVM 459
           SASST LT AE +
Sbjct: 440 SASSTDLTAAETL 451

BLAST of Chy9G170470 vs. NCBI nr
Match: XP_004143840.3 (embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical protein Csa_000306 [Cucumis sativus])

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 448/459 (97.60%), Postives = 452/459 (98.47%), Query Frame = 0

Query: 1   MQIQDDGMIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSF 60
           +QIQDDG+IRILNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALRLSSF PSF
Sbjct: 22  IQIQDDGIIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTPSSSPLIALRLSSFTPSF 81

Query: 61  FLTNQRPRSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLRLTTDTVV MAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 82  FLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 141

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP
Sbjct: 142 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 201

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 202 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 261

Query: 241 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 262 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 321

Query: 301 DKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSK 360
           DKSLAGALRKIFNKHAALFTDM  EFNIRLAAKATTVRDYDDALTRVSFGFN+VDEYYSK
Sbjct: 322 DKSLAGALRKIFNKHAALFTDMTGEFNIRLAAKATTVRDYDDALTRVSFGFNTVDEYYSK 381

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA
Sbjct: 382 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 441

Query: 421 PLGFPWTDPFVIDFLMYLENQKFKASASSTGLTTAEVMT 459
           PLGFPWTDPFVIDFLMYLEN+KFKASASST LT AEVMT
Sbjct: 442 PLGFPWTDPFVIDFLMYLENKKFKASASSTDLTMAEVMT 480

BLAST of Chy9G170470 vs. NCBI nr
Match: KAA0042649.1 (embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa])

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 438/459 (95.42%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 1   MQIQDDGMIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSF 60
           MQIQDD M   LNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALR+SSF PSF
Sbjct: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60

Query: 61  FLTNQRPRSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLR TT +VVAMAE+PSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWV GDDLRLPLDSPVLILLP
Sbjct: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240

Query: 241 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           +RYPKAN+YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300

Query: 301 DKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSK 360
           DKSLA ALRKIFNKHAALFTDMPSEFNIRLAAKA TVRDYDDALTRVSFGFN+VDEYYSK
Sbjct: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDI+ENPNCMLIVTP+GGHLGWVAGPGA
Sbjct: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420

Query: 421 PLGFPWTDPFVIDFLMYLENQKFKASASSTGLTTAEVMT 459
           PLGFPWTDPFVIDFLMYLENQK KASASST LTTAEVMT
Sbjct: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 459

BLAST of Chy9G170470 vs. NCBI nr
Match: XP_008437445.1 (PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis melo])

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 432/452 (95.58%), Postives = 440/452 (97.35%), Query Frame = 0

Query: 8   MIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRP 67
           M   LNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALR+SSF PSFFLTNQRP
Sbjct: 1   MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP 60

Query: 68  RSLRLTTDTVVAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 127
           RSLR TT +VVAMAE+PSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH
Sbjct: 61  RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 120

Query: 128 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQ 187
           LETIFASFFRTCPSVNLHRECLRAADNGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ 180

Query: 188 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKAN 247
           DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT+RYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN 240

Query: 248 LYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGA 307
           +YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLA A
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA 300

Query: 308 LRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFI 367
           LRKIFNKHAALFTDMPSEFNIRLAAKA TVRDYDDALTRVSFGFN+VDEYYSKSSSSKFI
Sbjct: 301 LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI 360

Query: 368 KDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWT 427
           KDVKIPLLCIQAANDPIAPNRGIPREDI+ENPNCMLIVTP+GGHLGWVAGPGAPLGFPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT 420

Query: 428 DPFVIDFLMYLENQKFKASASSTGLTTAEVMT 459
           DPFVIDFLMYLENQK KASASST LTTAEVMT
Sbjct: 421 DPFVIDFLMYLENQKSKASASSTDLTTAEVMT 452

BLAST of Chy9G170470 vs. NCBI nr
Match: XP_038875981.1 (embryogenesis-associated protein EMB8 [Benincasa hispida])

HSP 1 Score: 791 bits (2043), Expect = 3.71e-287
Identity = 388/426 (91.08%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 34  MARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRPRSLRLTTDTVVAMAELPSEKRIKPHP 93
           MARAFLGST SSSPLIALR SSF PSF +TNQR RSLR T  TV+AMAELPSEKRI+PHP
Sbjct: 1   MARAFLGSTHSSSPLIALRFSSFTPSF-ITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60

Query: 94  SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 153
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120

Query: 154 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 213
           NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180

Query: 214 GNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPL 273
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKAN+YAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240

Query: 274 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMPSEFNIRLAAK 333
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LA AL KIFNKHA LF DMP EFNIRLAA 
Sbjct: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300

Query: 334 ATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 393
           A TVRDYD+ALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360

Query: 394 DIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKFKASASSTGLT 453
           DI+ENPNC+LIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN   KASASS GLT
Sbjct: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420

Query: 454 TAEVMT 459
            AEVMT
Sbjct: 421 PAEVMT 425

BLAST of Chy9G170470 vs. NCBI nr
Match: XP_022949852.1 (embryogenesis-associated protein EMB8-like [Cucurbita moschata])

HSP 1 Score: 762 bits (1968), Expect = 2.72e-275
Identity = 370/433 (85.45%), Postives = 395/433 (91.22%), Query Frame = 0

Query: 26  PIPSHSPPMARAFLGSTLSSSPLIALRLSSFNPSFFLTNQRPRSLRLTTDTVVAMAELPS 85
           PIP H PPMARAFLGST SSS L   RLSSF PS  + NQR RSLRLTT  VVAM+E PS
Sbjct: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSL-INNQRRRSLRLTTAAVVAMSEFPS 79

Query: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145
           EK I+PHPSLEVIGGGCD+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 80  EKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139

Query: 146 RECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199

Query: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLG 265
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT+RYPKAN+YAVGWSLGGNILVNYLG
Sbjct: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259

Query: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMPSE 325
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LA ALR+IF+KHA LF DMP E
Sbjct: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319

Query: 326 FNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385
           FNI LAA A TVR+YDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379

Query: 386 PNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKFKA 445
           P+RG+PREDI+ENPNC+LIVTPKGGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+ KA
Sbjct: 380 PDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439

Query: 446 SASSTGLTTAEVM 458
           SASST LT AE +
Sbjct: 440 SASSTDLTAAETL 451

BLAST of Chy9G170470 vs. TAIR 10
Match: AT3G50790.1 (esterase/lipase/thioesterase family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 5.8e-160
Identity = 264/366 (72.13%), Postives = 305/366 (83.33%), Query Frame = 0

Query: 78  VAMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKD-LDLPYKPFPVIGSNRHLETIFASFF 137
           V  A  P   R   H SLEVIGGG D+FLP+ KD L  PY  FP+IG NRH+ETI+ASFF
Sbjct: 26  VFAAANPEMSRPSHHSSLEVIGGGSDRFLPSLKDSLAKPYNAFPLIGFNRHVETIYASFF 85

Query: 138 RTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLV 197
           R+ P V L RECLR  DNG+VALDWVAG+D   P DSP+LILLPGLTGGSQDSYVRHML+
Sbjct: 86  RSVPFVRLRRECLRTKDNGSVALDWVAGEDRHFPPDSPILILLPGLTGGSQDSYVRHMLL 145

Query: 198 RARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLG 257
           RA+ + WR VVFNSRGCG+SPVTTPQFYSASFLGD+ EV+ HV +++PKANLYA GWSLG
Sbjct: 146 RAQSKKWRCVVFNSRGCGDSPVTTPQFYSASFLGDIGEVIDHVVDKFPKANLYAAGWSLG 205

Query: 258 GNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHA 317
           GNILVNYLGQ+S  CPL+ AVSLCNPFDL++ADEDF KGFNNVYDK+L+ +LR+IF+KH+
Sbjct: 206 GNILVNYLGQESHNCPLTAAVSLCNPFDLVIADEDFHKGFNNVYDKALSKSLRRIFSKHS 265

Query: 318 ALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLC 377
            LF D+  EFNI LAA A TVRD+DD LTRVSFGF SVDEYYSKSSSSK IK V+IPLLC
Sbjct: 266 LLFEDIGGEFNIPLAANAETVRDFDDGLTRVSFGFKSVDEYYSKSSSSKAIKHVRIPLLC 325

Query: 378 IQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLM 437
           IQAANDPIAP RGIPR+DI+ NPNC+LIVTP+GGHLGWVAG  AP G PWTD  V++FL 
Sbjct: 326 IQAANDPIAPERGIPRDDIKANPNCVLIVTPRGGHLGWVAGEEAPNGAPWTDQVVMEFLQ 385

Query: 438 YLENQK 443
           ++EN++
Sbjct: 386 HVENRE 391

BLAST of Chy9G170470 vs. TAIR 10
Match: AT5G49950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 3.7e-58
Identity = 111/307 (36.16%), Postives = 176/307 (57.33%), Query Frame = 0

Query: 124 SNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLD----------- 183
           S+ HL+T F + F   P  +  R   +A D GT+ALDW+   D+   +            
Sbjct: 87  SSPHLQTAFLTLFGKSPPFSYKRILYQATDGGTIALDWLMHSDVVEGISQVVNASNPGTD 146

Query: 184 -SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGD 243
            +P+ I++PGLT  S  +Y++H+  R    GW VVV N RG G   +T+   Y+A +  D
Sbjct: 147 RTPIAIIVPGLTSDSSAAYIKHIAFRLAKEGWNVVVQNHRGLGGISLTSDCVYTAGWTED 206

Query: 244 MREVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADED 303
           +R+V+AH+  ++P+A L+AVG S+G N+LV YLG+D    PL GA ++C+P+DL++ D  
Sbjct: 207 LRKVIAHIHSQFPEAPLFAVGTSIGANVLVKYLGEDGPNTPLIGATAVCSPWDLLICDRF 266

Query: 304 F-RKGFNNVYDKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFG 363
             RK    VYD+ L   L+     H ++ + +     I+   K+ +VR++D+  TR+   
Sbjct: 267 INRKLVQKVYDRMLTIGLQGYAQLHHSIISRIADWEGIK---KSRSVREFDNYATRLVAK 326

Query: 364 FNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGG 418
           F + D YY +SSSS+++++V +PLLCI A +DP+     IP ++   N N +L  T  GG
Sbjct: 327 FETTDTYYRRSSSSQYVENVAVPLLCISALDDPVCTREAIPWDECRANKNIVLATTTHGG 386

BLAST of Chy9G170470 vs. TAIR 10
Match: AT1G34340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.9e-54
Identity = 107/306 (34.97%), Postives = 171/306 (55.88%), Query Frame = 0

Query: 127 HLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLD-------------- 186
           H++T F +F    P     R+    +D GT+ALDW+   D+   LD              
Sbjct: 96  HIQTCFLNFHGLPPVFTYTRQLFLTSDGGTIALDWLTNSDV---LDGSLHNKSEITKEDT 155

Query: 187 SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDM 246
           +P+ +++PGLT  S  +Y++H+       GW VV+ N RG G   VT+  FY+A +  D+
Sbjct: 156 TPIAVVIPGLTSDSSSAYLKHLAYDTAKTGWNVVISNHRGLGGVSVTSDCFYNAGWTDDI 215

Query: 247 REVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDF 306
           R V+ ++  +YP+A L+A+G S+G N+LV YLG++    PL GAV++C+P+DL++ D   
Sbjct: 216 RVVLDYLQHKYPRAPLFAIGTSIGANVLVKYLGEEGEKTPLRGAVAICSPWDLLIGDRFI 275

Query: 307 -RKGFNNVYDKSLAGALRKIFNKHAALFTDMPSEFNIRLAAKATTVRDYDDALTRVSFGF 366
            R     +YDK+L   L+     H   F  + +   I+   K+ ++RD+D+  T +   F
Sbjct: 276 CRTLKQKLYDKALTIGLQGYAQLHEPQFLRLANWEGIK---KSRSIRDFDNHATCLVGKF 335

Query: 367 NSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGH 418
            +VD YY KSSS++++ +V +PLLCI A +DP+     IP ++   N N +L  T  GGH
Sbjct: 336 ETVDTYYRKSSSTQYVGNVAVPLLCISALDDPLCTKEAIPWDECRANKNIVLATTNHGGH 395

BLAST of Chy9G170470 vs. TAIR 10
Match: AT2G03140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 8.7e-39
Identity = 95/270 (35.19%), Postives = 144/270 (53.33%), Query Frame = 0

Query: 146 RECLRAADNGTVALDWVAGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGW 205
           R C+   D G V+LDW A  D+R    LD+ V + +PG   GS +  VR  +  A  RG 
Sbjct: 172 RVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEALRRGV 231

Query: 206 RVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNY 265
             VV N RGC  SP+TTP+ ++A    D+   +  +++  P   L AVG   G N+L  Y
Sbjct: 232 FPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANMLTKY 291

Query: 266 LGQDSLTCPLSGAVSLCNPFDLIVADEDFRKG-FNNVYDKSLAGALRKIFNKHAALFTDM 325
           L +     PL+ AV + NPFDL   +E  R   ++   D+ L   L +I   +  LF   
Sbjct: 292 LAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKELFQGR 351

Query: 326 PSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAAND 385
              F++  A  + +VR++D AL+ V++G  S++++YS  ++ + I +VK+PLL IQ  ND
Sbjct: 352 AKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ--ND 411

Query: 386 PIAPNRGIPREDIEENPNCMLIVTPKGGHL 413
            + P   IPR  I ENP   L++     +L
Sbjct: 412 DVVPPYTIPRSSIAENPFTSLLLCSSSPNL 435

BLAST of Chy9G170470 vs. TAIR 10
Match: AT2G03140.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 8.7e-39
Identity = 95/270 (35.19%), Postives = 144/270 (53.33%), Query Frame = 0

Query: 146 RECLRAADNGTVALDWVAGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGW 205
           R C+   D G V+LDW A  D+R    LD+ V + +PG   GS +  VR  +  A  RG 
Sbjct: 172 RVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEALRRGV 231

Query: 206 RVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNY 265
             VV N RGC  SP+TTP+ ++A    D+   +  +++  P   L AVG   G N+L  Y
Sbjct: 232 FPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANMLTKY 291

Query: 266 LGQDSLTCPLSGAVSLCNPFDLIVADEDFRKG-FNNVYDKSLAGALRKIFNKHAALFTDM 325
           L +     PL+ AV + NPFDL   +E  R   ++   D+ L   L +I   +  LF   
Sbjct: 292 LAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKELFQGR 351

Query: 326 PSEFNIRLAAKATTVRDYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAAND 385
              F++  A  + +VR++D AL+ V++G  S++++YS  ++ + I +VK+PLL IQ  ND
Sbjct: 352 AKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ--ND 411

Query: 386 PIAPNRGIPREDIEENPNCMLIVTPKGGHL 413
            + P   IPR  I ENP   L++     +L
Sbjct: 412 DVVPPYTIPRSSIAENPFTSLLLCSSSPNL 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q408631.3e-12757.78Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1[more]
Q0VC001.5e-5134.13Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1[more]
Q8WU675.6e-5134.13Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2[more]
Q91ZH78.1e-5033.45Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1[more]
Q54H383.2e-4633.53Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=ab... [more]
Match NameE-valueIdentityDescription
A0A0A0KNN22.2e-26397.39AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148... [more]
A0A5A7TMR03.6e-25895.42Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AU571.1e-25495.58LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=36... [more]
A0A6J1GD898.1e-21885.45embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1IJD92.6e-21685.22embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_004143840.30.097.60embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical... [more]
KAA0042649.10.095.42embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 emb... [more]
XP_008437445.10.095.58PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis m... [more]
XP_038875981.13.71e-28791.08embryogenesis-associated protein EMB8 [Benincasa hispida][more]
XP_022949852.12.72e-27585.45embryogenesis-associated protein EMB8-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G50790.15.8e-16072.13esterase/lipase/thioesterase family protein [more]
AT5G49950.13.7e-5836.16alpha/beta-Hydrolases superfamily protein [more]
AT1G34340.11.9e-5434.97alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.18.7e-3935.19alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.28.7e-3935.19alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 174..412
e-value: 5.9E-21
score: 75.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 163..416
e-value: 2.5E-25
score: 91.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 139..421
NoneNo IPR availablePANTHERPTHR10794ABHYDROLASE DOMAIN-CONTAINING PROTEINcoord: 69..441
NoneNo IPR availablePANTHERPTHR10794:SF81EMBRYOGENESIS-ASSOCIATED PROTEIN EMB8-LIKEcoord: 69..441
IPR000952AB hydrolase 4, conserved sitePROSITEPS01133UPF0017coord: 341..382

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G170470.1Chy9G170470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044255 cellular lipid metabolic process
molecular_function GO:0047372 acylglycerol lipase activity
molecular_function GO:0034338 short-chain carboxylesterase activity