Chy9G160460 (gene) Cucumber (hystrix) v1

Overview
NameChy9G160460
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description2-methylene-furan-3-one reductase-like
LocationchrH09: 2865074 .. 2867054 (+)
RNA-Seq ExpressionChy9G160460
SyntenyChy9G160460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCGTTCCTATTCCCACCCACATGAAGGCTTGGGTTTACCATGACTTTGGCAACACCCCAGATGTTCTTAAACTGGATTTCAATTACCCAGTTCCTCAAATCAATGAAAACCAGTTGCTCATCAAGGTTGTTGCTGCATCTTTGAATCCCATTGATTACAAGAGAATCCATGGAGCTTTGAAAGCTTTTAACTATTTCCCTCCGGTCAGCTCTCTCTCTCTCTCTCTCTTTTCATTTAATTTTTACTTGGGTATCATTCTTTTGATCTGAACTTTTCTGGGTCTGTTTTTTATTTTGCATATATGGAATGAAATTATTAGTTCTAGATTTAGGTTATTCTCCATGAGCGTTGGAAATGGAAGAGGACATTATACGTGGATTAGAATATGGGTTTTTTTTTTTTACGAAGATTCCCCTTGCATAGGTATGTGACTCTTCGAACGACCAAGTATGCCATCGTTGGGTTATTTATGTTTTTTATCCTTGATTTAGAGACGTAGACGACATGTGTTCTACTTAAACTCTAACTCTAGAGATGCCTCTTTTTTTTATTCTGAATAATTCTCTCTAATAAAATTGTTCTCTAACACACTATCAGTGAACTATGTAGATACATTTGTTGATTCTTTACTGTTTATTACTATTTGTCGATTTCTTAACAACATTTTCAAGAATTTGGACAAATCTAAAACAGGAATGTTAGTTACAGGAGTTCATGGTAGATGATTAACTATTTCACAGATGCAAAATCAGGGTGGCTTTTTGAGATGATTTTGTTTGTGTAATTGCAAATAATCTCATGTGGTTTGGGGGAAGATTGGCTTCCTGATACTGATTTTTCTGTTTCTTTCTTTACTTGATTTGCTCTTACAGTCAATACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAATTCAAGGTAGGAGATGAAGTTTATGGAGATATAAACCATAAAGCATTGGATAAGCCAAAAAATGTTGGCACTTTGGCTGAGTACACTGCCACAGAAGAGAACGTTTTGGCACTGAAGCCTAAGAATCTGAGTTTCTTGGAGGCTGCTAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTTGAACGGGCCGGGCTTTCCCCTGGAAAATCGATTATTGTTTTGGGAGGTGCAGGGGGAGTCGGAGCTTTTATTATACAGGTAAACAATGGCTATGCTATTACATTCATTATTTGGAAGTTGGATCTGTTGTTTTTGTTGACTCCCATATTGATGTACAAAAGTTGTCTTTTGAGTGTATTAGCTCAACTATAATTGTATAACTTTCCTCACTTGAGATCTGAGGTTCGATCTTCCATCCTTACCGTTGCACTAAGAATGTTGTCTTCTGGGATTTTTTTTCTTTCTTTTCATTATTGGCTAATGTGATCTTGGTATTTGTTTTTCTGTATGAGGCAGCTTGCAAAGCATGTGTTTGGTGCATCAAAAGTAGTTGCTACTGCAAGCACAACAAAACAGGATCTCTTGAGAAGCTTAGGAGCTGATCAACCTATTGATTACACAAAGGAAAACTTTGAAGACCTTGATGAAAAATTCGATGTAGTCTACGACGCAGTCGGTAAGAAAATCAGTCATTTTCTTCAGATTTCTGCATCTGAAGTGTGAATGTGAAAATGTTTACTAGTGCAGTCTTGATTTAAAAACTGCAGGTCAGCCTGATAAAGCGGTTAAGGCGTTGAAAGAAGGCGGAAGTCTTGTGAGCATTGCTGCACCAGTTCCAGGGTTCCCGGATGCTTTCTTCCTCCTCACTTCAGATGCTGTCGTGTTGGAGAAACTAAATCCTTATCTGGAAAGTGGGAAGGTGAAACCGCTTATCGACCCCAAATCTCCATTCCCATTCTCTGATACCCTCGACGCATTCGCGTATCTCGAAACCTCCAGAGCTACTGGAAAAATTGTGATTTATCCCATCCCTTAG

mRNA sequence

ATGGCTGCCGTTCCTATTCCCACCCACATGAAGGCTTGGGTTTACCATGACTTTGGCAACACCCCAGATGTTCTTAAACTGGATTTCAATTACCCAGTTCCTCAAATCAATGAAAACCAGTTGCTCATCAAGGTTGTTGCTGCATCTTTGAATCCCATTGATTACAAGAGAATCCATGGAGCTTTGAAAGCTTTTAACTATTTCCCTCCGTCAATACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAATTCAAGGTAGGAGATGAAGTTTATGGAGATATAAACCATAAAGCATTGGATAAGCCAAAAAATGTTGGCACTTTGGCTGAGTACACTGCCACAGAAGAGAACGTTTTGGCACTGAAGCCTAAGAATCTGAGTTTCTTGGAGGCTGCTAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTTGAACGGGCCGGGCTTTCCCCTGGAAAATCGATTATTGTTTTGGGAGGTGCAGGGGGAGTCGGAGCTTTTATTATACAGCTTGCAAAGCATGTGTTTGGTGCATCAAAAGTAGTTGCTACTGCAAGCACAACAAAACAGGATCTCTTGAGAAGCTTAGGAGCTGATCAACCTATTGATTACACAAAGGAAAACTTTGAAGACCTTGATGAAAAATTCGATGTAGTCTACGACGCAGTCGGTCAGCCTGATAAAGCGGTTAAGGCGTTGAAAGAAGGCGGAAGTCTTGTGAGCATTGCTGCACCAGTTCCAGGGTTCCCGGATGCTTTCTTCCTCCTCACTTCAGATGCTGTCGTGTTGGAGAAACTAAATCCTTATCTGGAAAGTGGGAAGGTGAAACCGCTTATCGACCCCAAATCTCCATTCCCATTCTCTGATACCCTCGACGCATTCGCGTATCTCGAAACCTCCAGAGCTACTGGAAAAATTGTGATTTATCCCATCCCTTAG

Coding sequence (CDS)

ATGGCTGCCGTTCCTATTCCCACCCACATGAAGGCTTGGGTTTACCATGACTTTGGCAACACCCCAGATGTTCTTAAACTGGATTTCAATTACCCAGTTCCTCAAATCAATGAAAACCAGTTGCTCATCAAGGTTGTTGCTGCATCTTTGAATCCCATTGATTACAAGAGAATCCATGGAGCTTTGAAAGCTTTTAACTATTTCCCTCCGTCAATACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAATTCAAGGTAGGAGATGAAGTTTATGGAGATATAAACCATAAAGCATTGGATAAGCCAAAAAATGTTGGCACTTTGGCTGAGTACACTGCCACAGAAGAGAACGTTTTGGCACTGAAGCCTAAGAATCTGAGTTTCTTGGAGGCTGCTAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTTGAACGGGCCGGGCTTTCCCCTGGAAAATCGATTATTGTTTTGGGAGGTGCAGGGGGAGTCGGAGCTTTTATTATACAGCTTGCAAAGCATGTGTTTGGTGCATCAAAAGTAGTTGCTACTGCAAGCACAACAAAACAGGATCTCTTGAGAAGCTTAGGAGCTGATCAACCTATTGATTACACAAAGGAAAACTTTGAAGACCTTGATGAAAAATTCGATGTAGTCTACGACGCAGTCGGTCAGCCTGATAAAGCGGTTAAGGCGTTGAAAGAAGGCGGAAGTCTTGTGAGCATTGCTGCACCAGTTCCAGGGTTCCCGGATGCTTTCTTCCTCCTCACTTCAGATGCTGTCGTGTTGGAGAAACTAAATCCTTATCTGGAAAGTGGGAAGGTGAAACCGCTTATCGACCCCAAATCTCCATTCCCATTCTCTGATACCCTCGACGCATTCGCGTATCTCGAAACCTCCAGAGCTACTGGAAAAATTGTGATTTATCCCATCCCTTAG

Protein sequence

MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHGALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLAKHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKALKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLDAFAYLETSRATGKIVIYPIP*
Homology
BLAST of Chy9G160460 vs. ExPASy Swiss-Prot
Match: Q941I0 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 8.3e-118
Identity = 225/324 (69.44%), Postives = 259/324 (79.94%), Query Frame = 0

Query: 1   MAAVP---IPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKR 60
           MAA P   IP+  KAWVY ++G T DVLK D +  VP+I E+Q+LIKVVAASLNP+D+KR
Sbjct: 1   MAAAPSESIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEIKEDQVLIKVVAASLNPVDFKR 60

Query: 61  IHGALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAE 120
             G  K  +   P+IPGYDVAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAE
Sbjct: 61  ALGYFKDTDSPLPTIPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNETALVNPTRFGSLAE 120

Query: 121 YTATEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFII 180
           YTA  E VLA KPKNLSF+EAASLPLA+ETA+EGLERA LS GKS++VLGGAGGVG  II
Sbjct: 121 YTAAYERVLAHKPKNLSFIEAASLPLAIETAHEGLERAELSAGKSVLVLGGAGGVGTHII 180

Query: 181 QLAKHVFGASKVVATASTTKQDLLRSLG-ADQPIDYTKENFEDLDEKFDVVYDAVGQPDK 240
           QLAKHVFGASKV ATAST K DLLR+LG AD  IDYTKENFEDL EKFDVVYDAVG+ DK
Sbjct: 181 QLAKHVFGASKVAATASTKKLDLLRTLGAADLAIDYTKENFEDLPEKFDVVYDAVGETDK 240

Query: 241 AVKALKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFS 300
           AVKA+KEGG +V+I  P    P   F+LTS   VLEKL PYLESGKVKP++DP SP+PF+
Sbjct: 241 AVKAVKEGGKVVTIVGPATP-PAILFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFT 300

Query: 301 DTLDAFAYLETSRATGKIVIYPIP 321
             ++AF YLE+SRATGK+V+YPIP
Sbjct: 301 KVVEAFGYLESSRATGKVVVYPIP 323

BLAST of Chy9G160460 vs. ExPASy Swiss-Prot
Match: Q84V25 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-117
Identity = 221/323 (68.42%), Postives = 257/323 (79.57%), Query Frame = 0

Query: 1   MAAVP---IPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKR 60
           MAA P   IP+  KAWVY ++G T DVLK D +  VP++ E+Q+LIKVVAASLNP+D+KR
Sbjct: 1   MAAAPSESIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEVKEDQVLIKVVAASLNPVDFKR 60

Query: 61  IHGALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAE 120
             G  K  +   P++PGYDVAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAE
Sbjct: 61  ALGYFKDTDSPLPTVPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNEAALVNPTRFGSLAE 120

Query: 121 YTATEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFII 180
           YTA +E VLA KPK+LSF+EAASLPLA+ETAYEGLERA LS GKSI+VLGGAGGVG  II
Sbjct: 121 YTAADERVLAHKPKDLSFIEAASLPLAIETAYEGLERAELSAGKSILVLGGAGGVGTHII 180

Query: 181 QLAKHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKA 240
           QLAKHVFGASKV ATAST K D LR+LG D  IDYTKEN EDL EKFDVVYDAVG+ DKA
Sbjct: 181 QLAKHVFGASKVAATASTKKLDFLRTLGVDLAIDYTKENIEDLPEKFDVVYDAVGETDKA 240

Query: 241 VKALKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSD 300
           VKA+KEGG +V+I  P    P   F+LTS   VLEKL PYLESGKVKP++DP SP+PF+ 
Sbjct: 241 VKAVKEGGKVVTIVGPATP-PAIHFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTK 300

Query: 301 TLDAFAYLETSRATGKIVIYPIP 321
            ++AF YLE+SRATGK+V+YPIP
Sbjct: 301 LVEAFGYLESSRATGKVVVYPIP 322

BLAST of Chy9G160460 vs. ExPASy Swiss-Prot
Match: O23939 (2-methylene-furan-3-one reductase OS=Fragaria vesca OX=57918 GN=EO PE=2 SV=2)

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-117
Identity = 223/323 (69.04%), Postives = 258/323 (79.88%), Query Frame = 0

Query: 1   MAAVP---IPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKR 60
           MAA P   IP+  KAWV  ++G T DVLK D +  VP+I E+Q+LIKVVAASLNP+D+KR
Sbjct: 1   MAAAPSESIPSVNKAWVXSEYGKTSDVLKFDPSVAVPEIKEDQVLIKVVAASLNPVDFKR 60

Query: 61  IHGALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAE 120
             G  K  +   P+IPGY VAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAE
Sbjct: 61  ALGYFKDTDSPLPTIPGYYVAGVVVKVGSQVTKFKVGDEVYGDLNETALVNPTRFGSLAE 120

Query: 121 YTATEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFII 180
           YTA +E VLA KPKNLSF+EAASLPLA+ETA+EGLERA LS GKS++VLGGAGGVG  II
Sbjct: 121 YTAADERVLAHKPKNLSFIEAASLPLAIETAHEGLERAELSAGKSVLVLGGAGGVGTHII 180

Query: 181 QLAKHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKA 240
           QLAKHVFGASKV ATAST K DLLR+LGAD  IDYTKENFEDL EKFDVVYDAVG+ DKA
Sbjct: 181 QLAKHVFGASKVAATASTKKLDLLRTLGADLAIDYTKENFEDLPEKFDVVYDAVGETDKA 240

Query: 241 VKALKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSD 300
           VKA+KEGG +V+I  P    P   F+LTS   VLEKL PYLESGKVKP++DP SP+PF+ 
Sbjct: 241 VKAVKEGGKVVTIVGPATP-PAILFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTK 300

Query: 301 TLDAFAYLETSRATGKIVIYPIP 321
            ++AF YLE+SRATGK+V+YPIP
Sbjct: 301 VVEAFGYLESSRATGKVVVYPIP 322

BLAST of Chy9G160460 vs. ExPASy Swiss-Prot
Match: K4BW79 (2-methylene-furan-3-one reductase OS=Solanum lycopersicum OX=4081 GN=EO PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.0e-117
Identity = 217/320 (67.81%), Postives = 262/320 (81.88%), Query Frame = 0

Query: 3   AVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHGAL 62
           ++ IP+ MKAW Y D+G+  DVLKL+ N  VP I E+Q+LIK+VAA+LNP+D+KR  G  
Sbjct: 73  SISIPSEMKAWSYTDYGSV-DVLKLESNVAVPDIKEDQVLIKIVAAALNPVDFKRRLGKF 132

Query: 63  KAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATE 122
           KA +   P++PGYDVAGVVVKVGS+VK  K GDEVYGDI+ KALD PK  G+LAEYTA E
Sbjct: 133 KATDSPLPTVPGYDVAGVVVKVGSQVKGLKEGDEVYGDIHEKALDGPKQFGSLAEYTAVE 192

Query: 123 ENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLAKH 182
           E ++ALKPKNLSF EAA+LPLA+ETAYEGLE+AG S GKSI+VLGGAGGVG+ +IQLAKH
Sbjct: 193 EKLVALKPKNLSFAEAAALPLAIETAYEGLEKAGFSSGKSILVLGGAGGVGSLVIQLAKH 252

Query: 183 VFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKALK 242
           VFGASKV AT+ST K +LL+SLGAD  IDYTKENFEDL +KFDVVYD+VGQ +KAVK +K
Sbjct: 253 VFGASKVAATSSTGKLELLKSLGADLAIDYTKENFEDLPDKFDVVYDSVGQGEKAVKVVK 312

Query: 243 EGGSLVSIAAPV--PGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 302
           EGGS+V +   V  PGF    F++TS+  +L+KLNPYLESGKVKP+IDPK PF F   +D
Sbjct: 313 EGGSVVVLTGAVTPPGFR---FVVTSNGEMLKKLNPYLESGKVKPVIDPKGPFSFDKVVD 372

Query: 303 AFAYLETSRATGKIVIYPIP 321
           AF+YLET RATGK+VI+PIP
Sbjct: 373 AFSYLETGRATGKVVIHPIP 388

BLAST of Chy9G160460 vs. ExPASy Swiss-Prot
Match: Q9ZUC1 (NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AOR PE=1 SV=2)

HSP 1 Score: 399.1 bits (1024), Expect = 4.9e-110
Identity = 206/321 (64.17%), Postives = 250/321 (77.88%), Query Frame = 0

Query: 2   AAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHGA 61
           A   IP  MKAWVY D+G   DVLKL+ N  VP+I E+Q+LIKVVAA+LNP+D KR  G 
Sbjct: 70  ADASIPKEMKAWVYSDYGGV-DVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGK 129

Query: 62  LKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTAT 121
            KA +   P++PGYDVAGVVVKVGS VK  K GDEVY +++ KAL+ PK  G+LAEYTA 
Sbjct: 130 FKATDSPLPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAV 189

Query: 122 EENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLAK 181
           EE +LALKPKN+ F +AA LPLA+ETA EGL R   S GKSI+VL GAGGVG+ +IQLAK
Sbjct: 190 EEKLLALKPKNIDFAQAAGLPLAIETADEGLVRTEFSAGKSILVLNGAGGVGSLVIQLAK 249

Query: 182 HVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKAL 241
           HV+GASKV ATAST K +L+RSLGAD  IDYTKEN EDL +K+DVV+DA+G  DKAVK +
Sbjct: 250 HVYGASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAVKVI 309

Query: 242 KEGGSLVSIAAPV--PGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTL 301
           KEGG +V++   V  PGF    F++TS+  VL+KLNPY+ESGKVKP++DPK PFPFS   
Sbjct: 310 KEGGKVVALTGAVTPPGFR---FVVTSNGDVLKKLNPYIESGKVKPVVDPKGPFPFSRVA 369

Query: 302 DAFAYLETSRATGKIVIYPIP 321
           DAF+YLET+ ATGK+V+YPIP
Sbjct: 370 DAFSYLETNHATGKVVVYPIP 386

BLAST of Chy9G160460 vs. ExPASy TrEMBL
Match: E2S0A6 (Cytosolic alkenal/one oxidoreductase OS=Cucumis sativus OX=3659 GN=CsCytAOR PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.1e-176
Identity = 316/320 (98.75%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAAVPIPTHMKAWVYHDFGNTPDVLKLD NYPVPQINENQLLIKVVAASLNPIDYKRIHG
Sbjct: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDSNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKAFNYFPPSIPGYDVAGVV KVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYT 
Sbjct: 61  ALKAFNYFPPSIPGYDVAGVVAKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTV 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA
Sbjct: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           LKEGGSLVSIAAPVPGFPDAFFLLTSDAV+LEKLNPYLESGKVKPLIDPKSPFPFSDTLD
Sbjct: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVIYPIP 321
           AFAYLETSRATGKIVIYPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

BLAST of Chy9G160460 vs. ExPASy TrEMBL
Match: A0A5A7T3Y7 (2-methylene-furan-3-one reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00900 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.0e-174
Identity = 313/320 (97.81%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAAV IPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG
Sbjct: 1   MAAVSIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKS+IVLGGAGGVGA +IQLA
Sbjct: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSVIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K VFGASKVVATAST+KQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KQVFGASKVVATASTSKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           LKEGGSLVSIAAPVPGFPDAFFLLTSDAV+LEKLNPYLESGKVKPLIDPKSPFPFSDTLD
Sbjct: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVIYPIP 321
           AFAYLETSRATGKIVIYPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

BLAST of Chy9G160460 vs. ExPASy TrEMBL
Match: A0A1S3C8K8 (2-methylene-furan-3-one reductase-like OS=Cucumis melo OX=3656 GN=LOC103498067 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.0e-174
Identity = 313/320 (97.81%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAAV IPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG
Sbjct: 1   MAAVSIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKS+IVLGGAGGVGA +IQLA
Sbjct: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSVIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K VFGASKVVATAST+KQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KQVFGASKVVATASTSKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           LKEGGSLVSIAAPVPGFPDAFFLLTSDAV+LEKLNPYLESGKVKPLIDPKSPFPFSDTLD
Sbjct: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVIYPIP 321
           AFAYLETSRATGKIVIYPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

BLAST of Chy9G160460 vs. ExPASy TrEMBL
Match: A0A6J1FK96 (2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111444796 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.7e-163
Identity = 289/320 (90.31%), Postives = 307/320 (95.94%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAA+ +PTHMKAWVYH+FGNT DVLKLD NYPVP+INENQ+L+KVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           +LKA NY PPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  SLKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TE+ VLALKPKNLSFLEAASLPLAMETAYEGLERAGLS GKSIIVLGGAGGVGA +IQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K+VFGASK+ ATAST+KQDLLRSLGADQPIDYTKENFEDL EKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKENFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           +KEGG LVSIAAPV GFPDAFFLLTSDAV+LEKLNPYLESGKVKP+IDPKSPFPFSDTL+
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVILEKLNPYLESGKVKPIIDPKSPFPFSDTLE 300

Query: 301 AFAYLETSRATGKIVIYPIP 321
           AFAYLETSRATGKIV+YPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

BLAST of Chy9G160460 vs. ExPASy TrEMBL
Match: A0A6J1JTD8 (2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111489564 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.4e-162
Identity = 289/320 (90.31%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAA+ +PTHMKAWVYH+FGNT DVLKLD NYPVP+INENQ+L+KVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKA NY PPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TE+ VLALKPKNLSFLEAASLPLAMETAYEGLERAGLS GKSIIVLGGAGGVGA +IQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K+VFGASK+ ATAST+KQDLLRSLGADQPIDYTKE+FEDL EKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKESFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           +KEGG LVSIAAPV GFPDAFFLLTSDAV+LEKLNPYLESGKVKP+IDPKSPFPFSDTL 
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVMLEKLNPYLESGKVKPIIDPKSPFPFSDTLK 300

Query: 301 AFAYLETSRATGKIVIYPIP 321
           AFAYLETSRATGKIV+YPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

BLAST of Chy9G160460 vs. NCBI nr
Match: NP_001275527.1 (quinone oxidoreductase-like protein At1g23740, chloroplastic-like [Cucumis sativus] >KGN52377.1 hypothetical protein Csa_009027 [Cucumis sativus] >BAJ23911.1 cytosolic alkenal/one oxidoreductase [Cucumis sativus])

HSP 1 Score: 627 bits (1616), Expect = 3.23e-226
Identity = 316/320 (98.75%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAAVPIPTHMKAWVYHDFGNTPDVLKLD NYPVPQINENQLLIKVVAASLNPIDYKRIHG
Sbjct: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDSNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKAFNYFPPSIPGYDVAGVV KVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYT 
Sbjct: 61  ALKAFNYFPPSIPGYDVAGVVAKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTV 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA
Sbjct: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           LKEGGSLVSIAAPVPGFPDAFFLLTSDAV+LEKLNPYLESGKVKPLIDPKSPFPFSDTLD
Sbjct: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVIYPIP 320
           AFAYLETSRATGKIVIYPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

BLAST of Chy9G160460 vs. NCBI nr
Match: XP_008458755.1 (PREDICTED: 2-methylene-furan-3-one reductase-like [Cucumis melo] >KAA0037508.1 2-methylene-furan-3-one reductase-like [Cucumis melo var. makuwa] >TYK01983.1 2-methylene-furan-3-one reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 622 bits (1603), Expect = 3.10e-224
Identity = 313/320 (97.81%), Postives = 317/320 (99.06%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAAV IPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG
Sbjct: 1   MAAVSIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKS+IVLGGAGGVGA +IQLA
Sbjct: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSVIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K VFGASKVVATAST+KQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KQVFGASKVVATASTSKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           LKEGGSLVSIAAPVPGFPDAFFLLTSDAV+LEKLNPYLESGKVKPLIDPKSPFPFSDTLD
Sbjct: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVIYPIP 320
           AFAYLETSRATGKIVIYPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

BLAST of Chy9G160460 vs. NCBI nr
Match: XP_038890251.1 (2-methylene-furan-3-one reductase-like [Benincasa hispida])

HSP 1 Score: 596 bits (1536), Expect = 5.05e-214
Identity = 298/320 (93.12%), Postives = 311/320 (97.19%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAA+ +PTHMKAWVYH+FGNT DVLKLD NYPVP+INENQLLIKVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDSNYPVPRINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKAF+Y PP +PGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKAFDYSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TEE VLALKPKNLSFLEAASLPLAMETAYEGLERAGLS GKSIIVLGGAGGVGA++IQLA
Sbjct: 121 TEERVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           KHVFGASKVVATAST+KQDLLRSLGADQPIDYTKENFEDLDEKFD+VYDAVGQPDKAVKA
Sbjct: 181 KHVFGASKVVATASTSKQDLLRSLGADQPIDYTKENFEDLDEKFDIVYDAVGQPDKAVKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           LKEGG+LVSIA PVPGFPDAFFLLTSDAV+LEKLNPYLESGKVKP+ID KSPFPFSDTLD
Sbjct: 241 LKEGGNLVSIATPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPIIDSKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVIYPIP 320
           AFAYLETSRATGKIVIYPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

BLAST of Chy9G160460 vs. NCBI nr
Match: XP_023550772.1 (2-methylene-furan-3-one reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583 bits (1504), Expect = 3.80e-209
Identity = 290/320 (90.62%), Postives = 307/320 (95.94%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAA+ +PTHMKAWVYH+FGNT DVLKLD NYPVP+INENQ+L+KVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           ALKA NY PPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TE+ VLALKPKNLSFLEAASLPLAMETAYEGLERAGLS GKSIIVLGGAGGVGA +IQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K+VFGASK+ ATAST+KQDLLRSLGADQPIDYTKENFEDL EKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKENFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           +KEGG LVSIAAPV GFPDAFFLLTSDAV+LEKLNPYLESGKVKP+IDPKSPFPFSDTL+
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVILEKLNPYLESGKVKPIIDPKSPFPFSDTLE 300

Query: 301 AFAYLETSRATGKIVIYPIP 320
           AFAYLETSRATGKIV+YPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

BLAST of Chy9G160460 vs. NCBI nr
Match: XP_022938615.1 (2-methylene-furan-3-one reductase-like [Cucurbita moschata])

HSP 1 Score: 582 bits (1501), Expect = 1.09e-208
Identity = 289/320 (90.31%), Postives = 307/320 (95.94%), Query Frame = 0

Query: 1   MAAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHG 60
           MAA+ +PTHMKAWVYH+FGNT DVLKLD NYPVP+INENQ+L+KVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           +LKA NY PPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  SLKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLA 180
           TE+ VLALKPKNLSFLEAASLPLAMETAYEGLERAGLS GKSIIVLGGAGGVGA +IQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKA 240
           K+VFGASK+ ATAST+KQDLLRSLGADQPIDYTKENFEDL EKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKENFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 LKEGGSLVSIAAPVPGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTLD 300
           +KEGG LVSIAAPV GFPDAFFLLTSDAV+LEKLNPYLESGKVKP+IDPKSPFPFSDTL+
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVILEKLNPYLESGKVKPIIDPKSPFPFSDTLE 300

Query: 301 AFAYLETSRATGKIVIYPIP 320
           AFAYLETSRATGKIV+YPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

BLAST of Chy9G160460 vs. TAIR 10
Match: AT1G23740.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 399.1 bits (1024), Expect = 3.5e-111
Identity = 206/321 (64.17%), Postives = 250/321 (77.88%), Query Frame = 0

Query: 2   AAVPIPTHMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHGA 61
           A   IP  MKAWVY D+G   DVLKL+ N  VP+I E+Q+LIKVVAA+LNP+D KR  G 
Sbjct: 70  ADASIPKEMKAWVYSDYGGV-DVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGK 129

Query: 62  LKAFNYFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTAT 121
            KA +   P++PGYDVAGVVVKVGS VK  K GDEVY +++ KAL+ PK  G+LAEYTA 
Sbjct: 130 FKATDSPLPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAV 189

Query: 122 EENVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSPGKSIIVLGGAGGVGAFIIQLAK 181
           EE +LALKPKN+ F +AA LPLA+ETA EGL R   S GKSI+VL GAGGVG+ +IQLAK
Sbjct: 190 EEKLLALKPKNIDFAQAAGLPLAIETADEGLVRTEFSAGKSILVLNGAGGVGSLVIQLAK 249

Query: 182 HVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAVGQPDKAVKAL 241
           HV+GASKV ATAST K +L+RSLGAD  IDYTKEN EDL +K+DVV+DA+G  DKAVK +
Sbjct: 250 HVYGASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAVKVI 309

Query: 242 KEGGSLVSIAAPV--PGFPDAFFLLTSDAVVLEKLNPYLESGKVKPLIDPKSPFPFSDTL 301
           KEGG +V++   V  PGF    F++TS+  VL+KLNPY+ESGKVKP++DPK PFPFS   
Sbjct: 310 KEGGKVVALTGAVTPPGFR---FVVTSNGDVLKKLNPYIESGKVKPVVDPKGPFPFSRVA 369

Query: 302 DAFAYLETSRATGKIVIYPIP 321
           DAF+YLET+ ATGK+V+YPIP
Sbjct: 370 DAFSYLETNHATGKVVVYPIP 386

BLAST of Chy9G160460 vs. TAIR 10
Match: AT3G15090.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-33
Identity = 109/345 (31.59%), Postives = 170/345 (49.28%), Query Frame = 0

Query: 8   THMKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYK-RIHGALKAFN 67
           T  +A +   FG  P+V +L  N PVP +N N++L+K  A S+NP+D + R       F 
Sbjct: 30  TGCRAVILPRFGG-PEVFELRENVPVPNLNPNEVLVKAKAVSVNPLDCRIRAGYGRSVFQ 89

Query: 68  YFPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEENVL 127
              P I G DV+G V  +G+ VK  KVG EV+G ++  AL      GT  +Y    E+ L
Sbjct: 90  PHLPIIVGRDVSGEVAAIGTSVKSLKVGQEVFGALHPTALR-----GTYTDYGILSEDEL 149

Query: 128 ALKPKNLSFLEAASLPLAMETAYEGLE-RAGLSPGKSIIVLGGAGGVGAFIIQLAKHVFG 187
             KP ++S +EA+++P A  TA+  L+  A ++ G+ ++V GG G VG   IQLA  V  
Sbjct: 150 TEKPSSISHVEASAIPFAALTAWRALKSNARITEGQRLLVFGGGGAVGFSAIQLA--VAS 209

Query: 188 ASKVVATASTTKQDLLRSLGADQPIDYTKENFE-DLDEKFDVVYDAVGQPDK---AVKAL 247
              V A+     +D + + GA+Q +DYT E+ E  +  KFD V D +G P+     +  L
Sbjct: 210 GCHVTASCVGQTKDRILAAGAEQAVDYTTEDIELAVKGKFDAVLDTIGGPETERIGINFL 269

Query: 248 KEGGSLVSIAAP----------VPGFPDAFFLL--------------------TSDAVVL 307
           ++GG+ +++             V G P A  LL                     +D   L
Sbjct: 270 RKGGNYMTLQGEAASLTDKYGFVVGLPLATSLLMKKKIQYQYSHGIDYWWTYMRADPEGL 329

Query: 308 EKLNPYLESGKVKPLIDPKSPFPFSDTLDAFAYLETSRATGKIVI 317
            ++   + +GK+K  I  +  FP +D + A    E  +  GK+V+
Sbjct: 330 AEIQRLVGAGKLK--IPVEKTFPITDVVAAHEAKEKKQIPGKVVL 364

BLAST of Chy9G160460 vs. TAIR 10
Match: AT4G13010.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 134.0 bits (336), Expect = 2.1e-31
Identity = 115/341 (33.72%), Postives = 162/341 (47.51%), Query Frame = 0

Query: 10  MKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHGALKAFNYFP 69
           M A  Y+ +G     L+     PVP    N++ +K+ A SLNP+D+K   G ++ F   P
Sbjct: 6   MHALQYNSYGGGAAGLE-HVQVPVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRPF--LP 65

Query: 70  ---PSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEENVL 129
              P IP  DVAG VV+VGS VK FK GD+V   ++H         G LAE+    E + 
Sbjct: 66  RKFPCIPATDVAGEVVEVGSGVKNFKAGDKVVAVLSH------LGGGGLAEFAVATEKLT 125

Query: 130 ALKPKNLSFLEAASLPLAMETAYE------GLERAGLSPGKSIIVLGGAGGVGAFIIQLA 189
             +P+ +   EAA+LP+A  TA +      GL+  G     +I+V   +GGVG + +QLA
Sbjct: 126 VKRPQEVGAAEAAALPVAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLA 185

Query: 190 KHVFGASKVVATASTTKQDLLRSLGADQPIDYTKENFEDLDEKFDVVYDAV-----GQPD 249
           K     + V AT      + ++SLGAD+ +DY       L       YDAV     G P 
Sbjct: 186 K--LANAHVTATCGARNIEFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANGIPF 245

Query: 250 KAVKA-LKEGGSLVSIAAPVPGFPDAFF-----------------LLTSDAVVLEKLNPY 309
              +  L E G ++ I    PG P+A +                 LL   A  LE +   
Sbjct: 246 SVFEPNLSENGKVIDI---TPG-PNAMWTYAVKKITMSKKQLVPLLLIPKAENLEFMVNL 305

Query: 310 LESGKVKPLIDPKSPFPFSDTLDAFAYLETSRATGKIVIYP 319
           ++ GKVK +ID K   P S   DA+A      ATGKI++ P
Sbjct: 306 VKEGKVKTVIDSK--HPLSKAEDAWAKSIDGHATGKIIVEP 329

BLAST of Chy9G160460 vs. TAIR 10
Match: AT3G56460.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 107.1 bits (266), Expect = 2.7e-23
Identity = 78/245 (31.84%), Postives = 129/245 (52.65%), Query Frame = 0

Query: 19  GNTPDVLKLDFNYPVPQIN-ENQLLIKVVAASLNPIDYKRIHGALKAFNYFPPSIPGYDV 78
           G+    +++   +P+P +N +  + ++V+A SLN  +Y +I G  +      P IPG D 
Sbjct: 18  GSPESPVEVSKTHPIPSLNSDTSVRVRVIATSLNYANYLQILGKYQEKPPL-PFIPGSDY 77

Query: 79  AGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEENVLALKPKNLSFLE 138
           +G+V  +G  V KF+VGD V             ++G+ A++   +++ L L P+    + 
Sbjct: 78  SGIVDAIGPAVTKFRVGDRVCSF---------ADLGSFAQFIVADQSRLFLVPERCDMVA 137

Query: 139 AASLPLAMETAYEGL-ERAGLSPGKSIIVLGGAGGVGAFIIQLAKHVFGASKVVATASTT 198
           AA+LP+A  T++  L  RA L+ G+ ++VLG AGGVG   +Q+ K V GA  +     T 
Sbjct: 138 AAALPVAFGTSHVALVHRARLTSGQVLLVLGAAGGVGLAAVQIGK-VCGAIVIAVARGTE 197

Query: 199 KQDLLRSLGADQPIDYTKENFEDLDEKF---------DVVYDAVG--QPDKAVKALKEGG 251
           K  LL+S+G D  +D   EN     ++F         DV+YD VG     +++K LK G 
Sbjct: 198 KIQLLKSMGVDHVVDLGTENVISSVKEFIKTRKLKGVDVLYDPVGGKLTKESMKVLKWGA 251

BLAST of Chy9G160460 vs. TAIR 10
Match: AT4G21580.1 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 100.5 bits (249), Expect = 2.6e-21
Identity = 101/342 (29.53%), Postives = 161/342 (47.08%), Query Frame = 0

Query: 10  MKAWVYHDFGNTPDVLKLDFNYPVPQINENQLLIKVVAASLNPIDYKRIHGALKAFNYFP 69
           MKA V  + G  P+VL+L  +   P++ ++++LI+V+A +LN  D  +    L  +N  P
Sbjct: 1   MKAIVISEPGK-PEVLQLR-DVADPEVKDDEVLIRVLATALNRADTLQ---RLGLYNPPP 60

Query: 70  PSIP--GYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEENVLA 129
            S P  G + +G +  VG  V ++KVGD+V   ++          G  AE  +     + 
Sbjct: 61  GSSPYLGLECSGTIESVGKGVSRWKVGDQVCALLSG---------GGYAEKVSVPAGQIF 120

Query: 130 LKPKNLSFLEAASLPLAMETAYEGLERAG-LSPGKSIIVLGGAGGVGAFIIQLAKHVFGA 189
             P  +S  +AA+ P    T +  +   G LS G+S ++ GG+ G+G F IQ+AKH+ G 
Sbjct: 121 PIPAGISLKDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHL-GV 180

Query: 190 SKVVATASTTKQDLLRSLGADQPIDYTKENF------EDLDEKFDVVYDAVGQP--DKAV 249
              V   S  K    + LGAD  I+Y  E+F      E   +  DV+ D +G P   K +
Sbjct: 181 RVFVTAGSDEKLAACKELGADVCINYKTEDFVAKVKAETDGKGVDVILDCIGAPYLQKNL 240

Query: 250 KALKEGGSLVSIAAPVPGFPDAFFLLTS--------------------DAVVLEKLN--- 309
            +L   G L  I   + G  +A   L+S                     AVV+ ++    
Sbjct: 241 DSLNFDGRLCIIG--LMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVREVEKNV 300

Query: 310 -PYLESGKVKPLIDPKSPFPFSDTLDAFAYLETSRATGKIVI 317
            P +E+GKVKP+I      P S   +  + +E+S   GKI++
Sbjct: 301 WPAIEAGKVKPVI--YKYLPLSQAAEGHSLMESSNHIGKILL 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q941I08.3e-11869.442-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=2[more]
Q84V251.1e-11768.422-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1[more]
O239391.1e-11769.042-methylene-furan-3-one reductase OS=Fragaria vesca OX=57918 GN=EO PE=2 SV=2[more]
K4BW797.0e-11767.812-methylene-furan-3-one reductase OS=Solanum lycopersicum OX=4081 GN=EO PE=1 SV=... [more]
Q9ZUC14.9e-11064.17NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thalian... [more]
Match NameE-valueIdentityDescription
E2S0A63.1e-17698.75Cytosolic alkenal/one oxidoreductase OS=Cucumis sativus OX=3659 GN=CsCytAOR PE=2... [more]
A0A5A7T3Y71.0e-17497.812-methylene-furan-3-one reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3C8K81.0e-17497.812-methylene-furan-3-one reductase-like OS=Cucumis melo OX=3656 GN=LOC103498067 P... [more]
A0A6J1FK966.7e-16390.312-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1JTD84.4e-16290.312-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC1114895... [more]
Match NameE-valueIdentityDescription
NP_001275527.13.23e-22698.75quinone oxidoreductase-like protein At1g23740, chloroplastic-like [Cucumis sativ... [more]
XP_008458755.13.10e-22497.81PREDICTED: 2-methylene-furan-3-one reductase-like [Cucumis melo] >KAA0037508.1 2... [more]
XP_038890251.15.05e-21493.132-methylene-furan-3-one reductase-like [Benincasa hispida][more]
XP_023550772.13.80e-20990.632-methylene-furan-3-one reductase-like [Cucurbita pepo subsp. pepo][more]
XP_022938615.11.09e-20890.312-methylene-furan-3-one reductase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G23740.13.5e-11164.17Oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT3G15090.11.3e-3331.59GroES-like zinc-binding alcohol dehydrogenase family protein [more]
AT4G13010.12.1e-3133.72Oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT3G56460.12.7e-2331.84GroES-like zinc-binding alcohol dehydrogenase family protein [more]
AT4G21580.12.6e-2129.53oxidoreductase, zinc-binding dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020843Polyketide synthase, enoylreductase domainSMARTSM00829PKS_ER_names_modcoord: 20..316
e-value: 5.1E-14
score: 48.4
NoneNo IPR availablePFAMPF13602ADH_zinc_N_2coord: 204..316
e-value: 1.7E-19
score: 71.2
NoneNo IPR availableGENE3D3.90.180.10coord: 18..316
e-value: 1.8E-106
score: 357.7
NoneNo IPR availableGENE3D3.40.50.720coord: 152..267
e-value: 1.8E-106
score: 357.7
NoneNo IPR availablePANTHERPTHR44573:SF3CYTOSOLIC ALKENAL/ONE OXIDOREDUCTASEcoord: 3..320
NoneNo IPR availableCDDcd05289MDR_like_2coord: 10..316
e-value: 2.86585E-105
score: 308.335
IPR013154Alcohol dehydrogenase, N-terminalPFAMPF08240ADH_Ncoord: 39..101
e-value: 1.8E-9
score: 37.4
IPR044626NADPH-dependent oxidoreductase AOR-likePANTHERPTHR44573NADPH-DEPENDENT ALKENAL/ONE OXIDOREDUCTASE, CHLOROPLASTICcoord: 3..320
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 133..287
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 6..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G160460.1Chy9G160460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016491 oxidoreductase activity