Chy9G160140 (gene) Cucumber (hystrix) v1

Overview
NameChy9G160140
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionmetalloendoproteinase 2-MMP-like
LocationchrH09: 2585556 .. 2586849 (+)
RNA-Seq ExpressionChy9G160140
SyntenyChy9G160140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTTGAAACATTATTATCATTCTTGACAACCTCTTTTTTTCTTTTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAATAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAATGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCGAAATCCTTTGATGATAACGACTTCGACGGTGTTTTAGAGTCTGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTCTCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGGTTGCAGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACAGAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCTAAATCCTATCTCACTTATGGTACATTTCATCTTCTATTCATATATACTTTTCGTACATTTTTCATCTATATTTCAACAATATGGACTTTTTATGACACAAGACTAAAGGCAAATCAGTAGAGAGAAAAGACCGCCATAAAAGCTAAACTGGCTTTTTTTTTTTTTTTTTTTGGTGAAAAAGAACACTTCGTTTTCGAAAATTTCAAAATTTATGACAATTTTTCATGTCATGAATTTATGAAGGTAAATAATTATCATTAAAGTTTGATTATAACAAACTTGATCATCTATTTTTATACTATATTGATTTCATTGTTGAATTTAAAGTTATTATTATAACCTTTTATTCACATTCTTAACAGGATTTCTCTCAACAACCCCATCGGAGGCAGTAGCTCCGATAGGTCGAGCTTTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAGCATCTCAATACAAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGACCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAACAGATGGGAGATTACATTTCGACGCAGTTGAATCATGGGCTGTCGGCGCCGTTGCTGATTCGTTTGACTTGGAGACAGTAGCGTTACATGAGATTGGACACCTTCTCGGCCTCCATCACAGCTCCGTCGAAGGGGCTATTATGTGGCCAAGCATCATGGAAGGATCTACCAAAGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

mRNA sequence

ATGGCATTTGAAACATTATTATCATTCTTGACAACCTCTTTTTTTCTTTTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAATAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAATGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCGAAATCCTTTGATGATAACGACTTCGACGGTGTTTTAGAGTCTGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTCTCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGGTTGCAGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACAGAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCTAAATCCTATCTCACTTATGGATTTCTCTCAACAACCCCATCGGAGGCAGTAGCTCCGATAGGTCGAGCTTTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAGCATCTCAATACAAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGACCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAACAGATGGGAGATTACATTTCGACGCAGTTGAATCATGGGCTGTCGGCGCCGTTGCTGATTCGTTTGACTTGGAGACAGTAGCGTTACATGAGATTGGACACCTTCTCGGCCTCCATCACAGCTCCGTCGAAGGGGCTATTATGTGGCCAAGCATCATGGAAGGATCTACCAAAGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Coding sequence (CDS)

ATGGCATTTGAAACATTATTATCATTCTTGACAACCTCTTTTTTTCTTTTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAATAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAATGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCGAAATCCTTTGATGATAACGACTTCGACGGTGTTTTAGAGTCTGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTCTCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGGTTGCAGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACAGAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCTAAATCCTATCTCACTTATGGATTTCTCTCAACAACCCCATCGGAGGCAGTAGCTCCGATAGGTCGAGCTTTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAGCATCTCAATACAAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGACCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAACAGATGGGAGATTACATTTCGACGCAGTTGAATCATGGGCTGTCGGCGCCGTTGCTGATTCGTTTGACTTGGAGACAGTAGCGTTACATGAGATTGGACACCTTCTCGGCCTCCATCACAGCTCCGTCGAAGGGGCTATTATGTGGCCAAGCATCATGGAAGGATCTACCAAAGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Protein sequence

MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTPSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKGLDADDIAGINALYNAN*
Homology
BLAST of Chy9G160140 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-61
Identity = 124/285 (43.51%), Postives = 169/285 (59.30%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNL 101
           N  GC  G  V+G++++K+Y Q FGY+    + +S +F D DFD +L++A++ YQ N+NL
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD-DFDDILKAAVELYQTNFNL 105

Query: 102 KATGTLNAKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGN 161
             TG L+A T+  +  PRCG  D+V+G + M  G++   +      H H+V  Y  F G 
Sbjct: 106 NVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGE 165

Query: 162 PKWPASKSYLTYGFLSTTP--SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFES 221
           P+WP ++  LTY F    P   E  +   RAF  W   T  NF+ +  + T+DI I F +
Sbjct: 166 PRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYT 225

Query: 222 GDHGDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAVGAVADSF-------DLETVALHE 281
           GDHGD   FDGV G +AH F+P  G+ H DA E+W V    DSF       DLE+VA+HE
Sbjct: 226 GDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHE 285

Query: 282 IGHLLGLHHSSVEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           IGHLLGL HSSVE +IM+P+I  G  K  L  DD+ GI  LY AN
Sbjct: 286 IGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYGAN 326

BLAST of Chy9G160140 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.5e-60
Identity = 123/287 (42.86%), Postives = 169/287 (58.89%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F D DFD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTD-DFDDILKNAVEMYQRNFQL 107

Query: 102 KATGTLNAKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKING-HFHSVSHYAFFDG 161
             TG L+  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSYLTYGF--LSTTPSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFE 221
            P+WP ++  LTY F   +    E  +   RAF  W   T   F++  ++ T+DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 SGDHGDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAVG--------AVADSFDLETVAL 281
           SG+HGD   FDG    +AH F+P  G  H D  E+W V         +V+++ DLE+VA+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLHHSSVEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSSVEG+IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

BLAST of Chy9G160140 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.1e-52
Identity = 128/327 (39.14%), Postives = 168/327 (51.38%), Query Frame = 0

Query: 5   TLLSFLTTSFFLLIVLFPLISSSHPNSHLS---NGLVFLKNLNGCKKGDKVEGIHQMKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRCG 124
            + FGY+       +     +DFD VL+SAI TYQ N+NLK TG L++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 VADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFL--STT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F   +  
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGF 244
             E      RAF  W   T  NF+++     ADI I F SG+HGD   FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APTDGRLHFDAVESWAVG---------AVADSFDLETVALHEIGHLLGLHHSSVEGAIMW 304
           +P  G LH D  E W +           V    DLE+VA+HEIGHLLGL HSSVE AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

BLAST of Chy9G160140 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 120/280 (42.86%), Postives = 157/280 (56.07%), Query Frame = 0

Query: 49  GDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLN 108
           G  V G+ ++K YL  FGY+N+  + +S     + FDG LESAI  YQ N  L  TG L+
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASK 168
             T+ LMS PRCGV+D     T M      +N     N   H+ +HY +F+G PKW  ++
Sbjct: 124 TSTVTLMSLPRCGVSD-----THM-----TIN-----NDFLHTTAHYTYFNGKPKW--NR 183

Query: 169 SYLTYGFLST------TPSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDH 228
             LTY    T      T  +      RAF  W +    +F +   + TAD+KI F +GDH
Sbjct: 184 DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDH 243

Query: 229 GDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAV-----GAVADSFDLETVALHEIGHLL 288
           GD   FDGV G +AH FAP +GRLH DA E+W V     G+   + DLE+VA HEIGHLL
Sbjct: 244 GDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLL 303

Query: 289 GLHHSSVEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           GL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 GLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

BLAST of Chy9G160140 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 1.5e-50
Identity = 135/325 (41.54%), Postives = 168/325 (51.69%), Query Frame = 0

Query: 4   ETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKEYLQ 63
           + LL  L T +FL   L P +S+  P  +  +G    K       G   +G+  +K Y  
Sbjct: 7   QELLVALATLYFLATSL-PSVSAHGP--YAWDGEATYK-FTTYHPGQNYKGLSNVKNYFH 66

Query: 64  HFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRCGVA 123
           H GY+ N     +  FDDN FD  L SAIKTYQ NYNL  TG  +  TL  +  PRCGV 
Sbjct: 67  HLGYIPN-----APHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVP 126

Query: 124 DIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGF-----LST 183
           DI+    +  S              F  +S Y FF   P+W A  + LTY F     L  
Sbjct: 127 DIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDD 186

Query: 184 TPSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHG 243
           T   A+A   RAF  W    +  F + + Y+TA+IKI F S +HGD + FDG  G++ H 
Sbjct: 187 TFKSAIA---RAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHA 246

Query: 244 FAPTDGRLHFDAVESWAVGA------VADSFDLETVALHEIGHLLGLHHSSVEGAIMWPS 303
           FAPTDGR HFDA E W          V  +FDLE+VA+HEIGHLLGL HSS   AIM+PS
Sbjct: 247 FAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPS 304

Query: 304 IMEGSTK-GLDADDIAGINALYNAN 317
           I   + K  L  DDI GI  LY  N
Sbjct: 307 IPPRTRKVNLAQDDIDGIRKLYGIN 304

BLAST of Chy9G160140 vs. ExPASy TrEMBL
Match: A0A0A0KU08 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 5.8e-167
Identity = 291/316 (92.09%), Postives = 304/316 (96.20%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           MAFETLLSFLTTSFFL+IVLFPLISSSHP SHLSNGLVFLKNLNGCKKGDKVEGIHQ+KE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDD++FDGVLESAIKTYQLNYNLKATGT+NAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           G ADIVDGKTRMKSGKKMVNQ RKINGHFHSVSHYAFFDGNPKWPASKS+LTYGFL  TP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           S+AVA IGRAFKTWGANTHFNFSQ S+YK ADIKISFE+GDHGD HSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of Chy9G160140 vs. ExPASy TrEMBL
Match: A0A5D3BMC5 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00180 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 285/316 (90.19%), Postives = 303/316 (95.89%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           M FE LLSFLTTSFFL+IVLFPLISSSHPN H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDD++FDGVLESAIKTYQLNYNLKATGTL+AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           GVADI+DGKTRMKSGKKMVNQ RKINGHFH+VSHYAFFDGNPKWPASKS+LTYGFL  TP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           S+AVAP+GRAFKTWGA+THFNFS+AS+YK ADIKISF SGDHGD HSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDA+ESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of Chy9G160140 vs. ExPASy TrEMBL
Match: A0A1S3C9I6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 285/316 (90.19%), Postives = 303/316 (95.89%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           M FETLLSFLTTSFFL+IVLFPLISSSHPN H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDD++FDGVLESAIKTYQLNYNLKATGTL+AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           GVADI+DGKTRMKSGKKMVNQ RKINGHFH+VSHYAFFDGNPKWPASK +LTYGFL  TP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           S+AVAP+GRAFKTWGA+THFNFS+AS+YK ADIKISF SGDHGD HSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDA+ESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of Chy9G160140 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.5e-134
Identity = 241/313 (77.00%), Postives = 270/313 (86.26%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           MA    LSFL +S FLLI LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQMK 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YL+ FGYLN  VQN+SK++DD +FD VLESAI+TYQLNYNL+ATGTL+A TL LMS+PRC
Sbjct: 61  YLRRFGYLNR-VQNHSKTYDD-EFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           GVAD+  GKTRM SGKKMVN+ RKI+G FH VSH+AFFDGNPKWPASKS+LTYGFL  TP
Sbjct: 121 GVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           +EA+AP+GRAF TW ANTHF FS AS Y+TADIKISFESGDHGD  +FDGV GVIAH F+
Sbjct: 181 TEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDAVE WA+GA+ DSFD+ETVALHEIGHLLGL HSSVEGAIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALY 314
           L ADDIAGINALY
Sbjct: 301 LHADDIAGINALY 311

BLAST of Chy9G160140 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 6.7e-131
Identity = 237/315 (75.24%), Postives = 269/315 (85.40%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           MA    LSF  +S FLLI LFPLISSS      S+GL+FL NLNGCKKGD VEG+HQMK 
Sbjct: 1   MAVLQALSFFASSLFLLITLFPLISSSPQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YL  FGYL ++VQN+SK++DD +FD +LESAI+TYQLNYNL+ATGTL+A TL LMS+PRC
Sbjct: 61  YLCRFGYL-SHVQNHSKTYDD-EFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           GVAD+  GKT M SGKKMVN+ RKI+ HFH VSH+AFFDGNPKWPASKS+L YGFL  TP
Sbjct: 121 GVADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
            EA+AP+GRAF TW ANTHF FSQAS Y+TADIKISFESGDHGD  +FDGV GVIAH F+
Sbjct: 181 IEAIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFS 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDA+E+WA+GAV +SFD+ETVALHEIGHLLGLHHSSVEGAIMWP+IMEG+TKG
Sbjct: 241 PTDGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKG 300

Query: 301 LDADDIAGINALYNA 316
           L ADDIAGINALY A
Sbjct: 301 LHADDIAGINALYTA 313

BLAST of Chy9G160140 vs. NCBI nr
Match: XP_004142461.1 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52414.1 hypothetical protein Csa_009393 [Cucumis sativus])

HSP 1 Score: 597 bits (1538), Expect = 1.84e-214
Identity = 291/316 (92.09%), Postives = 304/316 (96.20%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           MAFETLLSFLTTSFFL+IVLFPLISSSHP SHLSNGLVFLKNLNGCKKGDKVEGIHQ+KE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDD++FDGVLESAIKTYQLNYNLKATGT+NAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           G ADIVDGKTRMKSGKKMVNQ RKINGHFHSVSHYAFFDGNPKWPASKS+LTYGFL  TP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           S+AVA IGRAFKTWGANTHFNFSQ S+YK ADIKISFE+GDHGD HSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 316
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of Chy9G160140 vs. NCBI nr
Match: XP_008458907.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 588 bits (1516), Expect = 4.14e-211
Identity = 285/316 (90.19%), Postives = 303/316 (95.89%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           M FETLLSFLTTSFFL+IVLFPLISSSHPN H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDD++FDGVLESAIKTYQLNYNLKATGTL+AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           GVADI+DGKTRMKSGKKMVNQ RKINGHFH+VSHYAFFDGNPKWPASK +LTYGFL  TP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           S+AVAP+GRAFKTWGA+THFNFS+AS+YK ADIKISF SGDHGD HSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDA+ESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 316
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of Chy9G160140 vs. NCBI nr
Match: KAA0037435.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00225.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 588 bits (1516), Expect = 4.14e-211
Identity = 285/316 (90.19%), Postives = 303/316 (95.89%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           M FE LLSFLTTSFFL+IVLFPLISSSHPN H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDD++FDGVLESAIKTYQLNYNLKATGTL+AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           GVADI+DGKTRMKSGKKMVNQ RKINGHFH+VSHYAFFDGNPKWPASKS+LTYGFL  TP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           S+AVAP+GRAFKTWGA+THFNFS+AS+YK ADIKISF SGDHGD HSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDA+ESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 316
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

BLAST of Chy9G160140 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 528 bits (1361), Expect = 1.65e-187
Identity = 261/313 (83.39%), Postives = 286/313 (91.37%), Query Frame = 0

Query: 1   MAFETLLSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKE 60
           MAFE L SF  TSFFLLI++FPLISSS PN+  S+GLVFL NL+GCKKGDKVEGIHQMK 
Sbjct: 1   MAFEGL-SFFITSFFLLIIIFPLISSSEPNNAHSHGLVFLNNLHGCKKGDKVEGIHQMKA 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRC 120
           YLQHFGYLN +VQN+SKSF D++FD VLESAIKTYQLNYNLKATGTL+A TL LMS+PRC
Sbjct: 61  YLQHFGYLN-HVQNHSKSFSDDEFDEVLESAIKTYQLNYNLKATGTLDATTLALMSKPRC 120

Query: 121 GVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTP 180
           G+AD+VDGKT MKSGKKMVNQ RKINGHFHSVSHYAFF+GNPKWPASKS+LTYGFL  TP
Sbjct: 121 GIADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFA 240
           SEAVAP+GRAF TW ANTHF+FSQAS Y+TADIKISFESGDHGD H+FDGV GVIAH F+
Sbjct: 181 SEAVAPVGRAFTTWAANTHFSFSQASAYETADIKISFESGDHGDGHAFDGVGGVIAHAFS 240

Query: 241 PTDGRLHFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKG 300
           PTDGRLHFDAVE WA+GAVADS+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM G+TKG
Sbjct: 241 PTDGRLHFDAVEPWAIGAVADSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKG 300

Query: 301 LDADDIAGINALY 313
           L ADDIAGINALY
Sbjct: 301 LHADDIAGINALY 311

BLAST of Chy9G160140 vs. NCBI nr
Match: XP_022938357.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 487 bits (1253), Expect = 4.62e-171
Identity = 239/307 (77.85%), Postives = 268/307 (87.30%), Query Frame = 0

Query: 7   LSFLTTSFFLLIVLFPLISSSHPNSHLSNGLVFLKNLNGCKKGDKVEGIHQMKEYLQHFG 66
           LSFL +S FLLI LFPLISSSH     S+GL+FL NLNGCKKGD VEG+HQMK YL+ FG
Sbjct: 7   LSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKRYLRRFG 66

Query: 67  YLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRCGVADIV 126
           YLN  VQN+SK++DD +FD VLESAI+TYQLNYNL+ATGTL+A TL LMS+PRCGVAD+ 
Sbjct: 67  YLNR-VQNHSKTYDD-EFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADVF 126

Query: 127 DGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFLSTTPSEAVAP 186
            GKTRM SGKKMVN+ RKI+G FH VSH+AFFDGNPKWPASKS+LTYGFL  TP+EA+AP
Sbjct: 127 HGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIAP 186

Query: 187 IGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGFAPTDGRL 246
           +GRAF TW ANTHF FS AS Y+TADIKISFESGDHGD  +FDGV GVIAH F+PTDGRL
Sbjct: 187 VGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGRL 246

Query: 247 HFDAVESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGSTKGLDADDI 306
           HFDAVE WA+GA+ DSFD+ETVALHEIGHLLGL HSSVEGAIMWP+IMEG+TKGL ADDI
Sbjct: 247 HFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADDI 306

Query: 307 AGINALY 313
           AGINALY
Sbjct: 307 AGINALY 311

BLAST of Chy9G160140 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-62
Identity = 124/285 (43.51%), Postives = 169/285 (59.30%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNL 101
           N  GC  G  V+G++++K+Y Q FGY+    + +S +F D DFD +L++A++ YQ N+NL
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD-DFDDILKAAVELYQTNFNL 105

Query: 102 KATGTLNAKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGN 161
             TG L+A T+  +  PRCG  D+V+G + M  G++   +      H H+V  Y  F G 
Sbjct: 106 NVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGE 165

Query: 162 PKWPASKSYLTYGFLSTTP--SEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFES 221
           P+WP ++  LTY F    P   E  +   RAF  W   T  NF+ +  + T+DI I F +
Sbjct: 166 PRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYT 225

Query: 222 GDHGDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAVGAVADSF-------DLETVALHE 281
           GDHGD   FDGV G +AH F+P  G+ H DA E+W V    DSF       DLE+VA+HE
Sbjct: 226 GDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHE 285

Query: 282 IGHLLGLHHSSVEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           IGHLLGL HSSVE +IM+P+I  G  K  L  DD+ GI  LY AN
Sbjct: 286 IGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYGAN 326

BLAST of Chy9G160140 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-61
Identity = 123/287 (42.86%), Postives = 169/287 (58.89%), Query Frame = 0

Query: 42  NLNGCKKGDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F D DFD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTD-DFDDILKNAVEMYQRNFQL 107

Query: 102 KATGTLNAKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKING-HFHSVSHYAFFDG 161
             TG L+  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSYLTYGF--LSTTPSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFE 221
            P+WP ++  LTY F   +    E  +   RAF  W   T   F++  ++ T+DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 SGDHGDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAVG--------AVADSFDLETVAL 281
           SG+HGD   FDG    +AH F+P  G  H D  E+W V         +V+++ DLE+VA+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLHHSSVEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSSVEG+IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

BLAST of Chy9G160140 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.5e-53
Identity = 128/327 (39.14%), Postives = 168/327 (51.38%), Query Frame = 0

Query: 5   TLLSFLTTSFFLLIVLFPLISSSHPNSHLS---NGLVFLKNLNGCKKGDKVEGIHQMKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDLMSRPRCG 124
            + FGY+       +     +DFD VL+SAI TYQ N+NLK TG L++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 VADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSYLTYGFL--STT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F   +  
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHSFDGVRGVIAHGF 244
             E      RAF  W   T  NF+++     ADI I F SG+HGD   FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APTDGRLHFDAVESWAVG---------AVADSFDLETVALHEIGHLLGLHHSSVEGAIMW 304
           +P  G LH D  E W +           V    DLE+VA+HEIGHLLGL HSSVE AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

BLAST of Chy9G160140 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 120/280 (42.86%), Postives = 157/280 (56.07%), Query Frame = 0

Query: 49  GDKVEGIHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLN 108
           G  V G+ ++K YL  FGY+N+  + +S     + FDG LESAI  YQ N  L  TG L+
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASK 168
             T+ LMS PRCGV+D     T M      +N     N   H+ +HY +F+G PKW  ++
Sbjct: 124 TSTVTLMSLPRCGVSD-----THM-----TIN-----NDFLHTTAHYTYFNGKPKW--NR 183

Query: 169 SYLTYGFLST------TPSEAVAPIGRAFKTWGANTHFNFSQASQYKTADIKISFESGDH 228
             LTY    T      T  +      RAF  W +    +F +   + TAD+KI F +GDH
Sbjct: 184 DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDH 243

Query: 229 GDDHSFDGVRGVIAHGFAPTDGRLHFDAVESWAV-----GAVADSFDLETVALHEIGHLL 288
           GD   FDGV G +AH FAP +GRLH DA E+W V     G+   + DLE+VA HEIGHLL
Sbjct: 244 GDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLL 303

Query: 289 GLHHSSVEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           GL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 GLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

BLAST of Chy9G160140 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-42
Identity = 107/274 (39.05%), Postives = 143/274 (52.19%), Query Frame = 0

Query: 55  IHQMKEYLQHFGYLNNYVQNYSKSFDDNDFDGVLESAIKTYQLNYNLKATGTLNAKTLDL 114
           I ++K +LQ +GYL       +K  DD  F    E A+  YQ N  L  TG  ++ TL  
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 115 MSRPRCGVADIVDGKTRMKSGKKMVNQQRKINGHFHSVSHYAFFDGNPKWPASKSY-LTY 174
           +  PRCG  D V+ KT                  FH+   Y +F G P+W       LTY
Sbjct: 110 ILLPRCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTY 169

Query: 175 GFLSTTPSEAVAP------IGRAFKTWGANTHFNFSQASQYKTADIKISFESGDHGDDHS 234
            F     +  +AP        RAF  W +    +F +   Y  ADIKI F +GDHGD   
Sbjct: 170 AFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEP 229

Query: 235 FDGVRGVIAHGFAPTDGRLHFDAVESWAVGAVAD----SFDLETVALHEIGHLLGLHHSS 294
           FDGV GV+AH F+P +GRLH D  E+WAV    +    + DLE+VA+HEIGH+LGL HSS
Sbjct: 230 FDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 295 VEGAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           V+ A M+P++   S K  L+ DD+ G+ +LY  N
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTN 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O045291.9e-6143.51Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q5XF513.5e-6042.86Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Q9ZUJ52.1e-5239.14Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O235071.7e-5142.86Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
P291361.5e-5041.54Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KU085.8e-16792.09ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632130 PE=3 S... [more]
A0A5D3BMC52.1e-16490.19Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C9I62.1e-16490.19metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV... [more]
A0A6J1FDT96.5e-13477.00metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P... [more]
A0A6J1JTF56.7e-13175.24metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=... [more]
Match NameE-valueIdentityDescription
XP_004142461.11.84e-21492.09metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52414.1 hypothetical protein C... [more]
XP_008458907.14.14e-21190.19PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo][more]
KAA0037435.14.14e-21190.19metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00225.1 metalloe... [more]
XP_038889209.11.65e-18783.39metalloendoproteinase 2-MMP-like [Benincasa hispida][more]
XP_022938357.14.62e-17177.85metalloendoproteinase 2-MMP-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G70170.11.3e-6243.51matrix metalloproteinase [more]
AT1G24140.12.5e-6142.86Matrixin family protein [more]
AT1G59970.11.5e-5339.14Matrixin family protein [more]
AT4G16640.11.2e-5242.86Matrixin family protein [more]
AT2G45040.11.2e-4239.05Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 187..202
score: 36.59
coord: 211..239
score: 52.73
coord: 301..314
score: 52.7
coord: 268..293
score: 57.13
coord: 115..128
score: 49.39
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 160..315
e-value: 1.4E-30
score: 117.6
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 42..316
e-value: 5.3E-74
score: 250.7
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 167..313
e-value: 2.3E-42
score: 144.5
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 53..115
e-value: 2.6E-10
score: 40.3
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 14..314
NoneNo IPR availablePANTHERPTHR10201:SF269METALLOENDOPROTEINASE 3-MMP-LIKEcoord: 14..314
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 157..316
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 163..313
e-value: 2.47816E-52
score: 167.381
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 46..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G160140.1Chy9G160140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity