Chy9G158350 (gene) Cucumber (hystrix) v1

Overview
NameChy9G158350
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationchrH09: 985812 .. 990453 (+)
RNA-Seq ExpressionChy9G158350
SyntenyChy9G158350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGAGAAAGAAAACCTGGCGCCAAGTTCAGTGAACTCTGTTTCAGTGCTGATTCCGCCGCACTTCGTTCTCGTTCATGGCATAAGTGGAGGAGGTTGGTGCTGGTACAAAATCAGATGTTTAATGGAGAATTCTGGCTTTAAAGTCACTTGTATTGATCTCAAAGGCGCTGGAATCGATCGCTCTGATCCGAATTCCGTCTTCAATTTCGACGATTACAATCAGCCTCTGCTCGATTTCATTTCTAGTTTGCCGGAAAACGAACAGGTAATTAATGTTTTTATTATACGTTCGTCGTTTTTTTTTGTTTGTTTGTTTGTAATTTTGTTTGTAAATCTCTGTGTGGAATCGATTGTTCCGATCTGATTTCCGTCGTTAGTTTAGACAATTACAATAAGCCTCTGCTCGATTTCATCTCTAGTTTAATAGAAAATGAGCAGGTAATTATATTGTTGTTATTATTATTATACATTCGTTGCTTCTTGTAATTTCATTTTCGTCTTTGGATGTGTCGTTCTTCAATCGATTGCGATCGAGTGAAGTTCTGTTTGATTTAGAGATCGTTGAAAACTTTTGCAGATCATTCTGGTTGGACATAGCGCTGGAGGATTAAGTATTACTCAAGCGACGCTTAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTATGTAGCGGCAACGATGCTGAGATTTGGATTCCAGACTGATCAAGATATTAAAGACGTAAGTAAATTCTCTTGTTCTCACTACTTTTAGTTCATGTTTGATTTTACGAATAGTAATTGAAGTAGAACTTGGAGTAGTAGTGCGGCGGCCGGACGACGAGGTTGTTGTGAAGCTTGTCGCCGGCCAGCGGTGGTGGTGCTGGCGATTATTTGCGAGTGAAAACGTGTGAATACTTTTGGCCGTTAGTCGAACCGCTAAGCTCTCTTGCTCTCATCGCAGATGGCATGAGGCAGAGAAAACGCCCCGCCTGCTAATTCATTCGGTTAAATTAGAAATTTAGTTAATAAAATTTAAAATTAGTCCCCATAATTTCAAATATTACAATATAGTCCTTAAAACGTAACAAGATTTTATAAACAAATCTTTAAGATTCGATAAAATTCTGCCACTAATTAAATTTTAATTTTCTTTGAAACGAAATTTATCATTTAATTATCATTAAAAAGCCACTAACTATTTATCGTTTTCTAGAAAAAAAAAGTCATTAGTCGGTTAATTAAACACTTTTGTTTTTGTCTTTTTAATTAGGAAATGAAATAGAGACAGCCACCAGCCAGCTGGTTCATGTAAACTAAACTTTTATTAACATTTTGATTAAGGATTCCATTATATTAAACAATCCAAATCCAGATTGTAAGACTTAAATCAGTGAAATGTTAATTGTCCAAGTGATTACTTCATTTTCTTTATTAAGTTTTTCATAAATTGAAGGTTTTTTCTTAAGAAATACCATAAAATAAATTAAAAGACATTGAATCACAAAAAATTACATCCTTCATACCTTTAATAAGCAAACAATACCATTCCTGAGGTTGAAGCTTAATTTCCACATAATAACAAGGATGTATGAAGAAGAATTTGTCAATCGATAATTTTAATATTTTCTTTAAGCAAGATGATATGATATATATCCTTGGTATTTAATATTTTTCTTAGGCAAACATCTACTTATATGCATCATTTTTTTTAAAACAAAAAAACAATTCAATCTTTTTAAAAAATAATAAAGAACTTTATTGAAGAGAGCGTCCAAGTTGTTTTCCTATAATAGGAAATAATTCAAAAGATGATTTTGTTGCGCATAAAAAGACTGAATTATGAAGTTTAAAAAATAAAGATAATTTGAGTTTGGCATAAACATAATTCAAAAGACTAAAAAACCAAAATTGTGTTAGATTTATGTTTAAAGGAAAAAAGAAAGTACATAAGTGATAAGTTGTGGTAATATAATGTAAAAGTGGAGGTCGCTTGAATGCGATTTTTATCCCGAAGTAGATTGGTACGGTGGAACCACAGCGGCAGCCAAGAACGTGTCCCCCTCTCTTTCGTGAAAAAAACCTTCCTTTAGCTGCTGTAATTTTTTGGTGCCACACCCTACGTTGTGGATAAAATGCTTTCTATATAATTTTTTACAAAATAAAATACATTTTACTCCAACACACATTTTCATAAATAAAAAAAATAATAAATTAATTAAAAATTTTAAATTAGAGGTGTAAGTCCAATCCAAATAATCCGACAACTTGAACGTTGAGATTGAAAAATTACGATCAACTCAACCTATATATATATATATATATATTATCTATTTAATAAAAATAAACTTAAAGAGTATTTTATTAAACAATCGCGTAGGAAGTTAAATGATTGTATAGAAAGTTATCCGATCGTATAAAAAATAAAGGATCGTTTGAAGGTAGAAAGCTAAACAATCGTATAAGAGGGTAAACGAACATTTAAAAAACTAAACGATCGTCTTGAAAGTGAAATATATATATATATATATAGTCCAACAACCCAACCCAACCCATATTTTACTGGTTATGTTGAAAAATTCATCTAGATTATTTGGGATGCCAACCCAATCCATTCACACCTCTAATTTACCTAACATACTTCTTATTCATCACATAATAGGTATCATAGGTAGATTCAAAGTTTTCTTTTCTTGTTGTTTAATTTTAACTTATATAAATTTAATATCTAATTGAGATTGAAACATTCCATTAGAAGAATATAGAGTACATTTAAATGAAAATTAAGAATGTAATTAATCATTATGTGTGTGTAATGTGTATAGTGGTAGAGTGGATGGAATATCTTTTGGTATTATATTATTCAGGAGTTGGAGTCAAAATTAGGTTAGGGGAGTTGGGAGTGTTGAAAGTCCACAAAAGTAATAGTGTTTAGTGTGCTGAATAGAAGGCGACAAGTTCACAGTTCTCACGTGTGCACTCAAATTGTATTCTATGGGTTTTTATTATATATATCTTTTTATTTTAAAATTTTTTAATGATGAAGGTAAAATAATAATAATAATAATAATAATAATAATACAAAAGCCATTCTATTTCTAATTCCCTATTGATTGGGCACAGGAATTCCTCCTGCTCTGCCCATGTGCCCCAATTTGATATGGACATCTCTTGTTTATTCAACTCTATTTTCTTTCTTTTCCGTTTTCGTTCCTACATTATTCAATATAATACTGTCCTACCTTTTTATTCCTCTCCCTGCTCATCTGCTTCAAATCTGTTGATGTGATTTTTCGAACCTTGGGTTTGTTTGTATTAATTTGTAAGTATAAATTATTTTAATTTAAATAAAGCAGTGAGGAATTTAAAATCAAATAAAAGAGTAATTTAAAAAATAATACTAATTTAATTACTTATAATATAGTTGGAGTTATTGATTCCACCAAGTTCAAAAGCGTTGGTGGTAGTCCAAACAATTCTTCTAGTTTTTCCCTCTCTCACTTCTCTCTAATCTCTACCTACCAATCCGGCTCGGGGAAACGCTTAGGAAACTTTTGAGGCAAAGAAAGGAAGAAAAAGAGGGAAGATTTTTTTTTACTAGATATGGCAAAATGAAAAGAAAAATGTTGACAACCACATTTCCTCACTTTATGGTGATTGATTGAAGTTGAGCTGTTGAGAGAGAGAGAAAGAGAGAGAATTGGGTTTTTTTTTTTTTTTTTTTTTTCTCTTTCTCTTTTTCTATTTTTTTAATGTATAAAATCAATGCATTTTTAGATAGAGACACGTGTGTTCTAAGCAATCGGGGAGTAAAATTTTCCAATGTCTTAGTACACACCCGTGTAACCTCCCTCCCTAATCTCAATCTATAGACCTTAGATAATTACACATTTTCTAGACCTTTACACAATTATTAATTCATGTGTTGACTTTGATAATCAATTAATGTGATCACATCAGTAGTGTGAAAAATGTTGTGTATAAGTCTTTTGCTAACTTATTCTTATCACGACACAACAAACAATTGAATAGTGGAGATGAAAGATGGAATCATAGTTTAATGAGATGGTATATCAATCATCATTAAGCTAAACTCATCTCAATTATGATTTTTAATGTGTAGGGAGTGCCAGATTTATCTGAATATGGAGATATATACAGCCTAGGATTTGGACTAGGATCCGAACAACCTCCCACCACTGCAGTCATCAAGAGGGAATTCCAACGTAAAATCGCCTATCACATGAGTCCCCAAGAGGTACCTAAACCCATTCTATAAAACAAATGAAATAAAGGACGTAGTGTTTGATAGTTATTAATTATATATATATATATGTGTGTGTGTGTGTGTGTGTTAGGATTCGACACTAGCAGCCATGCTACTGAGACCAGGGCCAATCCAGGCGTTAATGTCTGCAGAGTTCAGAGATGAGGAGCACAGCAGCAGGGAGGAGGGGGGAGAGAAGGTGAAAAGAGTGTATATAAGAACAATGTACGATCGAGTAATAAAACCGGAGCAACAAGAAGCCATGATAAAGAAATGGCCACCCGAAATCGTATACGAAATGGATACCGACCACAGCCCTTTTTTCTCCAACCCATCTCTACTCTTTGGCTTGCTTGTAAAAAGCTGTACCCTAAGTGGGGGGATATGTAATTAA

mRNA sequence

ATGGGAGAGAAAGAAAACCTGGCGCCAAGTTCAGTGAACTCTGTTTCAGTGCTGATTCCGCCGCACTTCGTTCTCGTTCATGGCATAAGTGGAGGAGGTTGGTGCTGGTACAAAATCAGATGTTTAATGGAGAATTCTGGCTTTAAAGTCACTTGTATTGATCTCAAAGGCGCTGGAATCGATCGCTCTGATCCGAATTCCGTCTTCAATTTCGACGATTACAATCAGCCTCTGCTCGATTTCATTTCTAGTTTGCCGGAAAACGAACAGATCATTCTGGTTGGACATAGCGCTGGAGGATTAAGTATTACTCAAGCGACGCTTAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTATGTAGCGGCAACGATGCTGAGATTTGGATTCCAGACTGATCAAGATATTAAAGACGGAGTGCCAGATTTATCTGAATATGGAGATATATACAGCCTAGGATTTGGACTAGGATCCGAACAACCTCCCACCACTGCAGTCATCAAGAGGGAATTCCAACGTAAAATCGCCTATCACATGAGTCCCCAAGAGGATTCGACACTAGCAGCCATGCTACTGAGACCAGGGCCAATCCAGGCGTTAATGTCTGCAGAGTTCAGAGATGAGGAGCACAGCAGCAGGGAGGAGGGGGGAGAGAAGGTGAAAAGAGTGTATATAAGAACAATGTACGATCGAGTAATAAAACCGGAGCAACAAGAAGCCATGATAAAGAAATGGCCACCCGAAATCGTATACGAAATGGATACCGACCACAGCCCTTTTTTCTCCAACCCATCTCTACTCTTTGGCTTGCTTGTAAAAAGCTGTACCCTAAGTGGGGGGATATGTAATTAA

Coding sequence (CDS)

ATGGGAGAGAAAGAAAACCTGGCGCCAAGTTCAGTGAACTCTGTTTCAGTGCTGATTCCGCCGCACTTCGTTCTCGTTCATGGCATAAGTGGAGGAGGTTGGTGCTGGTACAAAATCAGATGTTTAATGGAGAATTCTGGCTTTAAAGTCACTTGTATTGATCTCAAAGGCGCTGGAATCGATCGCTCTGATCCGAATTCCGTCTTCAATTTCGACGATTACAATCAGCCTCTGCTCGATTTCATTTCTAGTTTGCCGGAAAACGAACAGATCATTCTGGTTGGACATAGCGCTGGAGGATTAAGTATTACTCAAGCGACGCTTAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTATGTAGCGGCAACGATGCTGAGATTTGGATTCCAGACTGATCAAGATATTAAAGACGGAGTGCCAGATTTATCTGAATATGGAGATATATACAGCCTAGGATTTGGACTAGGATCCGAACAACCTCCCACCACTGCAGTCATCAAGAGGGAATTCCAACGTAAAATCGCCTATCACATGAGTCCCCAAGAGGATTCGACACTAGCAGCCATGCTACTGAGACCAGGGCCAATCCAGGCGTTAATGTCTGCAGAGTTCAGAGATGAGGAGCACAGCAGCAGGGAGGAGGGGGGAGAGAAGGTGAAAAGAGTGTATATAAGAACAATGTACGATCGAGTAATAAAACCGGAGCAACAAGAAGCCATGATAAAGAAATGGCCACCCGAAATCGTATACGAAATGGATACCGACCACAGCCCTTTTTTCTCCAACCCATCTCTACTCTTTGGCTTGCTTGTAAAAAGCTGTACCCTAAGTGGGGGGATATGTAATTAA

Protein sequence

MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLSGGICN*
Homology
BLAST of Chy9G158350 vs. ExPASy Swiss-Prot
Match: Q9SG92 (Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 5.3e-100
Identity = 166/261 (63.60%), Postives = 213/261 (81.61%), Query Frame = 0

Query: 20  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLL 79
           PPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YNQPL+
Sbjct: 18  PPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQPLI 77

Query: 80  DFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKDGV 139
           DF+SS PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G QTD+D+KDGV
Sbjct: 78  DFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKDGV 137

Query: 140 PDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQAL 199
           PDLSE+GD+Y LGFGLG E PPT+A+IK E++RK+ YHMSPQ++ +LAA+++RP PI AL
Sbjct: 138 PDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQECSLAALMMRPAPILAL 197

Query: 200 MSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEMDTDHSPF 259
            +A+  +EE    +E  E+V RVYI+T+ DRV+KPEQQ+AMI++WPP  VYE+++DHSPF
Sbjct: 198 TTAKLEEEEKEKGQE--EQVPRVYIKTLLDRVMKPEQQDAMIRRWPPSQVYELESDHSPF 257

Query: 260 FSNPSLLFGLLVKSCTLSGGI 281
           FSNP +LFGLL+K+    G I
Sbjct: 258 FSNPFVLFGLLIKAAVSVGSI 276

BLAST of Chy9G158350 vs. ExPASy Swiss-Prot
Match: Q2V0W1 (Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 8.5e-58
Identity = 113/253 (44.66%), Postives = 163/253 (64.43%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG S G WCWYK+  L+  +GFK T +DL GAGI+ +D N+VF+FD YN+PL   
Sbjct: 13  HFVFVHGASHGAWCWYKLTTLLVAAGFKATSVDLTGAGINLTDSNTVFDFDHYNRPLFSL 72

Query: 82  ISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKDGVPD 141
           +S LP + +I+LVGHS GG S+T+A  KF  KI + VY+AA M++ G  +     D +  
Sbjct: 73  LSDLPSHHKIVLVGHSIGGGSVTEALCKFTDKISMVVYLAADMVQPG--STSSTHDSIMT 132

Query: 142 LSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQALMS 201
           + E  DI+   +G G+++PPT  ++K EF+R   Y  SP ED +LA+ LLRP P++AL  
Sbjct: 133 VGE-EDIWEYIYGEGADKPPTGVLMKEEFRRHYYYSQSPLEDVSLASKLLRPAPVRALGG 192

Query: 202 AEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHSPFF 261
           A     +  S     EKV RVYI+T  D +  P +Q+ +++KWPP  +Y + ++DHS FF
Sbjct: 193 A-----DKLSPNPEAEKVPRVYIKTAKDNLFDPLRQDRLVEKWPPSQLYILEESDHSAFF 252

Query: 262 SNPSLLFGLLVKS 274
           S P+ LF  L+++
Sbjct: 253 SVPTTLFAYLLRA 257

BLAST of Chy9G158350 vs. ExPASy Swiss-Prot
Match: O23512 (Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.4e-54
Identity = 111/256 (43.36%), Postives = 158/256 (61.72%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI   D N VF+ D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  ISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTD---QDIKDG 141
           +S LP + ++ILVGHS GG S+T+A  KF  KI +A+Y+AA+M++ G        +I  G
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSNIHVG 131

Query: 142 VPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQA 201
             D+ EY       +G G+++PPT  ++K EF R   Y  SP ED TL++ LLRP P++A
Sbjct: 132 EEDIWEY------TYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVTLSSKLLRPAPMRA 191

Query: 202 LMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHS 261
                F+D +        EKV RVYI+T  D +    +Q+ +++ WPP  +Y + D+DHS
Sbjct: 192 -----FQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLYVLEDSDHS 251

Query: 262 PFFSNPSLLFGLLVKS 274
            FFS P+ LF  L+++
Sbjct: 252 AFFSVPTTLFAYLLRA 256

BLAST of Chy9G158350 vs. ExPASy Swiss-Prot
Match: Q9LVL9 (Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 109/260 (41.92%), Postives = 157/260 (60.38%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V + DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  ISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKDGVPD 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G    + +K+ +  
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 142 LS-----EYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPI 201
            S     E   I+   FG G +  PT+ ++K E+ R   Y+ SP ED TLA  LLRP P+
Sbjct: 125 CSGLIEEETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYTLATTLLRPAPV 184

Query: 202 QALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTD 261
            A +     D   +      +K+ RVY++T  D + +P  QE M+  WPP   + + D+D
Sbjct: 185 MAFIG--IMDIPGAPET---DKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTFLLPDSD 244

Query: 262 HSPFFSNPSLLFGLLVKSCT 276
           HS FFS P  L+  L+++ +
Sbjct: 245 HSAFFSQPQELYQFLLQAAS 259

BLAST of Chy9G158350 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.5e-43
Identity = 91/257 (35.41%), Postives = 150/257 (58.37%), Query Frame = 0

Query: 18  LIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQP 77
           LI   FVLVHG   G WCWYK+   +E SG     +DL G G + +D N+V   ++Y++P
Sbjct: 93  LIVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKP 152

Query: 78  LLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKD 137
           L+D + +LPE E++ILVGHS GG SI+ A  +F +KI  A++V ATM+  G +      +
Sbjct: 153 LIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSE 212

Query: 138 GVPDLSEY-GDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPI 197
            +     +  +   L +G G ++PPT  + ++   + + ++ SP +D  LA + +RP P+
Sbjct: 213 ELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPL 272

Query: 198 QALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTD 257
             +M      E+ S   E   K +R Y++T+ DR + P+ QE ++++  PE V+++  +D
Sbjct: 273 GPMM------EKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSD 332

Query: 258 HSPFFSNPSLLFGLLVK 273
           H PFFS P  L  +L++
Sbjct: 333 HCPFFSKPQSLHKILLE 343

BLAST of Chy9G158350 vs. ExPASy TrEMBL
Match: A0A5A7TBF5 (Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001670 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 7.6e-142
Identity = 252/277 (90.97%), Postives = 263/277 (94.95%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVS+  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV NFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIKDGVPDLSEYGDIY+L FGLG +QPPTTAVIKREFQRK+AYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAM 240
            EDSTLAAMLLRPGPIQALMSA+F++EE   REE  +KVKRVYIRTMYDRVIKPEQQEAM
Sbjct: 181 HEDSTLAAMLLRPGPIQALMSAQFKEEE--DREE-ADKVKRVYIRTMYDRVIKPEQQEAM 240

Query: 241 IKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLS 278
           IK WPPEIVYEMDTDHSPFFSNPSLLFGLL+KSC+L+
Sbjct: 241 IKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCSLN 274

BLAST of Chy9G158350 vs. ExPASy TrEMBL
Match: A0A1S3CAT2 (methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 7.6e-142
Identity = 252/277 (90.97%), Postives = 263/277 (94.95%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVS+  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV NFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIKDGVPDLSEYGDIY+L FGLG +QPPTTAVIKREFQRK+AYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAM 240
            EDSTLAAMLLRPGPIQALMSA+F++EE   REE  +KVKRVYIRTMYDRVIKPEQQEAM
Sbjct: 181 HEDSTLAAMLLRPGPIQALMSAQFKEEE--DREE-ADKVKRVYIRTMYDRVIKPEQQEAM 240

Query: 241 IKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLS 278
           IK WPPEIVYEMDTDHSPFFSNPSLLFGLL+KSC+L+
Sbjct: 241 IKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCSLN 274

BLAST of Chy9G158350 vs. ExPASy TrEMBL
Match: A0A6J1JV68 (methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.9e-130
Identity = 236/287 (82.23%), Postives = 251/287 (87.46%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+V  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIK+GVPDLSE+GD+Y +GFGLG +QPPTTAVIKRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGE--KVKRVYIRTMYDRVIKPEQQE 240
            EDSTLA+MLLRPGPI AL +A F       REE GE   VKRVYIRT YDRVIKPEQQE
Sbjct: 181 HEDSTLASMLLRPGPILALRTARF-------REENGELDTVKRVYIRTTYDRVIKPEQQE 240

Query: 241 AMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTL---SGGICN 283
           AMI++W PEIVYEMDTDHSPFFSNPSLLFGLLVK+      S   CN
Sbjct: 241 AMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAASSTAACN 280

BLAST of Chy9G158350 vs. ExPASy TrEMBL
Match: A0A6J1FL47 (methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.6e-129
Identity = 231/275 (84.00%), Postives = 247/275 (89.82%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+V  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIK+GVPDLSE+GD Y +GFG G +QPPTTAV+KRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGE--KVKRVYIRTMYDRVIKPEQQE 240
            EDSTLA+MLLRPGPI AL +A F       REE GE  +VKRVYIRT YDRVIKPEQQE
Sbjct: 181 HEDSTLASMLLRPGPILALRTARF-------REENGELDRVKRVYIRTTYDRVIKPEQQE 240

Query: 241 AMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKS 274
           AMI++W PEIVYEMDTDHSPFFSNPSLLFGLLVK+
Sbjct: 241 AMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKA 268

BLAST of Chy9G158350 vs. ExPASy TrEMBL
Match: A0A6J1BYB8 (methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.3e-129
Identity = 231/286 (80.77%), Postives = 256/286 (89.51%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60
           MGE++NLAPS+V SV+V  P    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEEDNLAPSAV-SVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRL 120
           GAGIDRSDPNSV  FDDYN+PLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRL
Sbjct: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAY 180
           AVYVAATML+ G+QTDQDIKDGVPDLSE+GD+Y L FGLGS+QPPTTA+IKREFQRKI Y
Sbjct: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180

Query: 181 HMSPQEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQ 240
           HMSP EDSTL AMLLRPGPI AL +A+FR+E     EE  +KVKRV+IRTMYDRVIK EQ
Sbjct: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREE-----EEFADKVKRVFIRTMYDRVIKREQ 240

Query: 241 QEAMIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLSGGICN 283
           Q+AMIK+WPPEIVY+MDTDHSPFFSNPSLLFGLL+K+   +G  CN
Sbjct: 241 QDAMIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTG--CN 278

BLAST of Chy9G158350 vs. NCBI nr
Match: XP_004141511.1 (methylesterase 17 [Cucumis sativus] >KAE8648969.1 hypothetical protein Csa_008717 [Cucumis sativus])

HSP 1 Score: 538 bits (1386), Expect = 2.18e-192
Identity = 267/280 (95.36%), Postives = 271/280 (96.79%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVSV IPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSVQIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           DRSDPNSVFNFDDYNQPLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DRSDPNSVFNFDDYNQPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQ DQDIKDGVPD SEYGD+YSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP
Sbjct: 121 AATMLRFGFQNDQDIKDGVPDFSEYGDVYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSS-REEGGEKVKRVYIRTMYDRVIKPEQQEA 240
           QEDSTLAAMLLRPGPIQALMSAEFRDEE SS  EEG EKVKRVYIRTMYDRVIKPEQQEA
Sbjct: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEERSSSEEEGAEKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLSGG 279
           MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKS TL+ G
Sbjct: 241 MIKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSSTLNMG 280

BLAST of Chy9G158350 vs. NCBI nr
Match: XP_008459483.1 (PREDICTED: methylesterase 17 [Cucumis melo] >KAA0039366.1 methylesterase 17 [Cucumis melo var. makuwa])

HSP 1 Score: 508 bits (1307), Expect = 1.60e-180
Identity = 252/277 (90.97%), Postives = 263/277 (94.95%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSVNSVS+  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV NFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIKDGVPDLSEYGDIY+L FGLG +QPPTTAVIKREFQRK+AYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAM 240
            EDSTLAAMLLRPGPIQALMSA+F++EE   REE  +KVKRVYIRTMYDRVIKPEQQEAM
Sbjct: 181 HEDSTLAAMLLRPGPIQALMSAQFKEEE--DREEA-DKVKRVYIRTMYDRVIKPEQQEAM 240

Query: 241 IKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLS 277
           IK WPPEIVYEMDTDHSPFFSNPSLLFGLL+KSC+L+
Sbjct: 241 IKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCSLN 274

BLAST of Chy9G158350 vs. NCBI nr
Match: XP_038890470.1 (methylesterase 17 [Benincasa hispida])

HSP 1 Score: 493 bits (1269), Expect = 1.06e-174
Identity = 244/282 (86.52%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSSV+SVSV  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVDSVSVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           DRSDPNSV  FDDYN+PLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV
Sbjct: 61  DRSDPNSVLKFDDYNKPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTD+D KDGVPDLSE+GD+Y + FGLG +QPPTTAVIKREFQRKI YHMSP
Sbjct: 121 AATMLRFGFQTDRDFKDGVPDLSEFGDVYDVEFGLGPQQPPTTAVIKREFQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAM 240
            EDSTLAAMLLRPGPI AL +A+F +E+     +G +KVKRVYIRTMYDRVIKPEQQ+AM
Sbjct: 181 HEDSTLAAMLLRPGPILALKTAQFMEEQ-----DGADKVKRVYIRTMYDRVIKPEQQDAM 240

Query: 241 IKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTLSGGICN 282
           IK WPPEIVYEMDTDHSPFFS+PSLLFGLLVKSC+LS G CN
Sbjct: 241 IKGWPPEIVYEMDTDHSPFFSDPSLLFGLLVKSCSLSMG-CN 276

BLAST of Chy9G158350 vs. NCBI nr
Match: XP_022993026.1 (methylesterase 17 [Cucurbita maxima])

HSP 1 Score: 468 bits (1205), Expect = 6.92e-165
Identity = 233/285 (81.75%), Postives = 250/285 (87.72%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+V  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIK+GVPDLSE+GD+Y +GFGLG +QPPTTAVIKRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAM 240
            EDSTLA+MLLRPGPI AL +A FR+E         + VKRVYIRT YDRVIKPEQQEAM
Sbjct: 181 HEDSTLASMLLRPGPILALRTARFREENGEL-----DTVKRVYIRTTYDRVIKPEQQEAM 240

Query: 241 IKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKSCTL---SGGICN 282
           I++W PEIVYEMDTDHSPFFSNPSLLFGLLVK+      S   CN
Sbjct: 241 IREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAASSTAACN 280

BLAST of Chy9G158350 vs. NCBI nr
Match: XP_022938905.1 (methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 465 bits (1196), Expect = 1.69e-163
Identity = 228/273 (83.52%), Postives = 246/273 (90.11%), Query Frame = 0

Query: 1   MGEKENLAPSSVNSVSVLIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+V  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DRSDPNSVFNFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120
           D SDPNSV  FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSP 180
           AATMLRFGFQTDQDIK+GVPDLSE+GD Y +GFG G +QPPTTAV+KRE QRKI YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180

Query: 181 QEDSTLAAMLLRPGPIQALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAM 240
            EDSTLA+MLLRPGPI AL +A FR+E         ++VKRVYIRT YDRVIKPEQQEAM
Sbjct: 181 HEDSTLASMLLRPGPILALRTARFREENGEL-----DRVKRVYIRTTYDRVIKPEQQEAM 240

Query: 241 IKKWPPEIVYEMDTDHSPFFSNPSLLFGLLVKS 273
           I++W PEIVYEMDTDHSPFFSNPSLLFGLLVK+
Sbjct: 241 IREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKA 268

BLAST of Chy9G158350 vs. TAIR 10
Match: AT3G10870.1 (methyl esterase 17 )

HSP 1 Score: 365.5 bits (937), Expect = 3.8e-101
Identity = 166/261 (63.60%), Postives = 213/261 (81.61%), Query Frame = 0

Query: 20  PPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLL 79
           PPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YNQPL+
Sbjct: 18  PPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQPLI 77

Query: 80  DFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKDGV 139
           DF+SS PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G QTD+D+KDGV
Sbjct: 78  DFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKDGV 137

Query: 140 PDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQAL 199
           PDLSE+GD+Y LGFGLG E PPT+A+IK E++RK+ YHMSPQ++ +LAA+++RP PI AL
Sbjct: 138 PDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQECSLAALMMRPAPILAL 197

Query: 200 MSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEMDTDHSPF 259
            +A+  +EE    +E  E+V RVYI+T+ DRV+KPEQQ+AMI++WPP  VYE+++DHSPF
Sbjct: 198 TTAKLEEEEKEKGQE--EQVPRVYIKTLLDRVMKPEQQDAMIRRWPPSQVYELESDHSPF 257

Query: 260 FSNPSLLFGLLVKSCTLSGGI 281
           FSNP +LFGLL+K+    G I
Sbjct: 258 FSNPFVLFGLLIKAAVSVGSI 276

BLAST of Chy9G158350 vs. TAIR 10
Match: AT4G16690.1 (methyl esterase 16 )

HSP 1 Score: 213.4 bits (542), Expect = 2.4e-55
Identity = 111/256 (43.36%), Postives = 158/256 (61.72%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI   D N VF+ D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  ISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTD---QDIKDG 141
           +S LP + ++ILVGHS GG S+T+A  KF  KI +A+Y+AA+M++ G        +I  G
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSNIHVG 131

Query: 142 VPDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQA 201
             D+ EY       +G G+++PPT  ++K EF R   Y  SP ED TL++ LLRP P++A
Sbjct: 132 EEDIWEY------TYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVTLSSKLLRPAPMRA 191

Query: 202 LMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHS 261
                F+D +        EKV RVYI+T  D +    +Q+ +++ WPP  +Y + D+DHS
Sbjct: 192 -----FQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLYVLEDSDHS 251

Query: 262 PFFSNPSLLFGLLVKS 274
            FFS P+ LF  L+++
Sbjct: 252 AFFSVPTTLFAYLLRA 256

BLAST of Chy9G158350 vs. TAIR 10
Match: AT5G58310.1 (methyl esterase 18 )

HSP 1 Score: 208.4 bits (529), Expect = 7.7e-54
Identity = 109/260 (41.92%), Postives = 157/260 (60.38%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V + DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  ISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKDGVPD 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G    + +K+ +  
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 142 LS-----EYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPI 201
            S     E   I+   FG G +  PT+ ++K E+ R   Y+ SP ED TLA  LLRP P+
Sbjct: 125 CSGLIEEETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYTLATTLLRPAPV 184

Query: 202 QALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTD 261
            A +     D   +      +K+ RVY++T  D + +P  QE M+  WPP   + + D+D
Sbjct: 185 MAFIG--IMDIPGAPET---DKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTFLLPDSD 244

Query: 262 HSPFFSNPSLLFGLLVKSCT 276
           HS FFS P  L+  L+++ +
Sbjct: 245 HSAFFSQPQELYQFLLQAAS 259

BLAST of Chy9G158350 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 176.4 bits (446), Expect = 3.2e-44
Identity = 91/257 (35.41%), Postives = 150/257 (58.37%), Query Frame = 0

Query: 18  LIPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQP 77
           LI   FVLVHG   G WCWYK+   +E SG     +DL G G + +D N+V   ++Y++P
Sbjct: 93  LIVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKP 152

Query: 78  LLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKD 137
           L+D + +LPE E++ILVGHS GG SI+ A  +F +KI  A++V ATM+  G +      +
Sbjct: 153 LIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSE 212

Query: 138 GVPDLSEY-GDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPI 197
            +     +  +   L +G G ++PPT  + ++   + + ++ SP +D  LA + +RP P+
Sbjct: 213 ELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPL 272

Query: 198 QALMSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTD 257
             +M      E+ S   E   K +R Y++T+ DR + P+ QE ++++  PE V+++  +D
Sbjct: 273 GPMM------EKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSD 332

Query: 258 HSPFFSNPSLLFGLLVK 273
           H PFFS P  L  +L++
Sbjct: 333 HCPFFSKPQSLHKILLE 343

BLAST of Chy9G158350 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 174.1 bits (440), Expect = 1.6e-43
Identity = 101/259 (39.00%), Postives = 138/259 (53.28%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDRSDPNSVFNFDDYNQPLLDF 81
           HFVLVHG S G WCWYK   L+E  GFKVT IDL G GI+  + N + +   Y +PL D 
Sbjct: 138 HFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDI 197

Query: 82  ISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYVAATMLRFGFQTDQ--DIKDGV 141
           +  LP  E++ILVGH  GG  I+ A   F  KI  AV++AA ML  G  T     +K G 
Sbjct: 198 LEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSLKAGQ 257

Query: 142 PDLSEYGDIYSLGFGLGSEQPPTTAVIKREFQRKIAYHMSPQEDSTLAAMLLRPGPIQAL 201
            DL     I+   +  G+E PPT   + +   + + ++ SP +D  LA++ +R  P   +
Sbjct: 258 NDLMRKAQIFI--YTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALASVSMRSIPFAPV 317

Query: 202 MSAEFRDEEHSSREEGGEKVKRVYIRTMYDRVIKPEQQEAMIKKWPPEIVYEM-DTDHSP 261
           +      E+ S  +     V+R YI T+ D  I    QE MI   PPE VY +   DH+P
Sbjct: 318 L------EKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGADHAP 377

Query: 262 FFSNPSLLFGLLVKSCTLS 278
           FFS P  L  LL++   +S
Sbjct: 378 FFSKPQALHKLLLEIARIS 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SG925.3e-10063.60Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1[more]
Q2V0W18.5e-5844.66Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1[more]
O235123.4e-5443.36Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1[more]
Q9LVL91.1e-5241.92Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1[more]
Q9FVW34.5e-4335.41Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
A0A5A7TBF57.6e-14290.97Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001... [more]
A0A1S3CAT27.6e-14290.97methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1[more]
A0A6J1JV683.9e-13082.23methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1[more]
A0A6J1FL475.6e-12984.00methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1[more]
A0A6J1BYB87.3e-12980.77methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141511.12.18e-19295.36methylesterase 17 [Cucumis sativus] >KAE8648969.1 hypothetical protein Csa_00871... [more]
XP_008459483.11.60e-18090.97PREDICTED: methylesterase 17 [Cucumis melo] >KAA0039366.1 methylesterase 17 [Cuc... [more]
XP_038890470.11.06e-17486.52methylesterase 17 [Benincasa hispida][more]
XP_022993026.16.92e-16581.75methylesterase 17 [Cucurbita maxima][more]
XP_022938905.11.69e-16383.52methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurb... [more]
Match NameE-valueIdentityDescription
AT3G10870.13.8e-10163.60methyl esterase 17 [more]
AT4G16690.12.4e-5543.36methyl esterase 16 [more]
AT5G58310.17.7e-5441.92methyl esterase 18 [more]
AT1G33990.13.2e-4435.41methyl esterase 14 [more]
AT3G29770.11.6e-4339.00methyl esterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 21..257
e-value: 1.3E-13
score: 51.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 20..274
e-value: 1.5E-56
score: 194.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 21..271
NoneNo IPR availablePANTHERPTHR10992:SF1043METHYLESTERASE 17-LIKEcoord: 21..273
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 21..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G158350.1Chy9G158350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033473 indoleacetic acid conjugate metabolic process
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
biological_process GO:0048367 shoot system development
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity