Chy9G157810 (gene) Cucumber (hystrix) v1

Overview
NameChy9G157810
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationchrH09: 567757 .. 571541 (-)
RNA-Seq ExpressionChy9G157810
SyntenyChy9G157810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGGTAATCAAAGCAAAATGATGAGGTGGTTGATATGGGCACTTTTCTTCCTCATAAGTACCCTTTCCTCTTCATCTTCTCCGGCACCGGCGGCTTTCATCGGAGTTCAAGACGGCCATTTTCAGCTAAAAGGTTCTCCATTTTTGTTCAATGGCTTTAACTCTTATTGGATGATGTCCGTCGCTACTGACCCGAACCAACGCAACAAAGTAACCCAAGTCTTTCGTGATGCCGCCACTGCCGGCCTCACCGTCTGCCGAACCTGGGCCTTCAACGACGGTGGCTTCCATGCCCTCCAAATCTCTCCCGGCGTTTATGATGAATCAGTTTTTCAGGTTAACTTTTTACTGATTTTCTTTTCTTCTTTACTCTCTAATTCCATTGCTTAAAAAAAAGTAATACTTCGATGCAGTTCATCAACGATCTTCAATTACAAATGAAAAATTATATTTTCTTAATAACAAAAAAAATGCTTATGGATTATCATTATACTTAGATGAATAATACAGAAATAAGTGTAACCTAAAATAGGTTTTTATTTAAGTTTGTGTTTATTATCTAATAAATCTTTTTTTTTTTTTGCTAATTAGATATTTTATCTATTTAAAATCTTTAAAAACTAACCAATTTATTTGTTATAAAATTGAATTTTATGGCTAATATAATAATCCTAAATAATTATTTTAACTTTATGTTTAATTGAATTTCGAAATGTTTGTAAAATACAATTAATAATCTAATTAGATATAAATTAAGAATTGAAAGACGGATTAAAATTAGTTTAAATATGATTCATATTTTTTTGACACCTTTTAAAGTTAAAGATTTGATATAGAAGCAATTAATTGATACAAAAAGATATTTAGTGACTAAATATACAATTTAATATCAATTTTTTATATTTAACATTTTTAATTATTGTTTCTAAATACTATATTATATTTTAAATATTTTAGAATTATATAATTTTATTTCCTTTTGAATAAGGAAACTCTCGGAATAGACGACAAGTACACAAAACAAAATCACAAGAATAATATATCTTATGAAAAGTCTTTTCCAAACTCTTAATTAATTCTTCTAACAAATAAATAACCCTTTTTATATATAAACCTCTAAATAATATTTTAATTAACTTCTTCTTTCTATCATCATCATCATAATAATAATAATAATAATAACAATAATAATTTCTTGTTGTGGTTAGATTTTGAATCATATTTCTTAATCAAGAAATTTATTGGCTCGATTATTATATATGTGTCGATCTTTTTTATAATTATTGTATCAATCAAGACTCTTTAATTTTTAGGGTTCTAATATGTTCTATATACATATATATATAATAATTTAGTTGTTAGTTTTATTATTATTACTATTTTGATGTACTTTTCAAATTGAATAATTCTTTTTTTTTTCCATTTTTTGCATAGCCTTTTAATTTGTTTCTTTTTCTTTTTTTAGTGACACTTATTGTTAAATGATATCTAACATTAAGTGAAGATTTATTAAATAATTTAAATTTTTTTAGTCAATCGGTTATTATATTTAATATAATATTAAAACGAATGATTTATAGGATAAGATTCATCTATTGAACTTTTGAGTTAATTTGTAATTTAAAACTTATATGTGTGTTTGCTTTATTATCCATTTTCTAAAGTCAAATTAAATAGAAGTAAAAGTTGTTTTCAAAACTGATTTTTGTCTCCGAGAATTTCTAGGTGACACCAAAATAGAAAAAGAATGTAAAGAAAAGAAAACATAAATATTAATTAAATACAGAAAATAAACAAAGAAATAATTGTTACCAAGCGATTCTAAATTCTATAATTTAAAATGTTTATAAACATGTTATTAAATGAACTCTTTGTTGAAATATAAGAAAAGTTCATCAAATCATATAGATTATTTACGAGGTTGACAAGTATAAAAATCTATAAAAATCTACAATCTTTTGATGGCTTTTAATTTAGAATATTGGTTGCCTGCAGGGTCTTGATTTCGTGATATTTGAAGCAAAAAAGTATGGAATTAGAATGATATTGAGTTTGGTAAATAATTTCAAAGACTACGGTGGAAGAGCAGCGTATGTCCGGTGGGCCGAAGCCGCCGGCGTTCAAGTTCATGACGAAGATGACTTCTACACTAATCAACTTATCAGAACATACTACAAAAACCATGTTCAGGTGATCAAATCTATACCCATATATATATATATATATATCTAAAACTAGCTACTATATTATTATTGGACTTGAAAACTGAACTAAAAATCAAACAGAACTCAATAATATTACTTCTATTTTGTTTTTGTAGAAAGTACTGAGAAGGAAGAACACAATGAATGGTTTGAGATATATGGAAGATCCAACCATTATGGGATGGGAACTTATGAACGAACCACGTTGTCAAGTTGATTCCTCCGGCAACACTGTCAATGTAACCTTTCATTATTTAACTTCTCTCCTTTTTCTCTTTTCCTTAATTAATCACTTTTATTTTTAGTGTCTAATCCTATTTTTATAACCTAAATTTTCATTTAAAAAGAGATAGAGGAAAATCTTATCTAATTTACGTAGACTATTGTATTTGATGTAATGTTGATCAAATAAAAGATTGAATCAAAGACGTTTAGAATGATAATGTTTTGTTCAAATGGACACATTGATTATTGACTTATTAGTGTACATAGTATTTGTTAGTACAAGTTGCATTATATAGCATTATATTTGAACTAAAAATAATTGCATATATATATAGAGAGCGCACAATGATAAAATAATTGTATACATAACACAAAAATCAAATCGGTAAAAGACTAAAAAATACATATCCAACTACCATTTCAAAAGATTCTATCTGATTTATCAAAAATTAAATGTGCCATCACTAGTATTCGTTGTCATCACATTGCCGATAGCTTTCAATTTAAGAAATGTACTATTAGTTTATATCATTGATAACGACTATTGGTGATTGTTTCTATCGGTTATAGACATGTGCCCTTATTTTTTTTAAAATTATACACTTATATGTAAGAGTTGTTTCCTAATGAAAATAGAGATGGGTAGAGGAGATGGGATCATATGTGAAATCAATTGACAAACAACATATGGTGGGGATTGGAATGGAAGGATTCTATGGAGATTCAAGTCCAAATAAAATCAAAGCCAACCCAGGTAGTTTCAAATTTGGCACTGATTTTATCACCAATAACCTCAACAAAGCAATCGACTTTGCAACAATTCATGTATATCCTGATGCGTGGTTAGTTTCTTCTTCCTCACTAATTATCAACTATTTCCTATTTTCATTACGTGGTACATGGTCCACAAAGTACTCCTTGAGTTTACAACTCAATATAATGTTAAATTTGTTTTCCATCGTTACTTATAGGCTACCAGGGAAGAGTGAAGCCACCAAAATGGCATTCCTTGAAGAATGGATAGCACTTCATTGGACGGACGCAAAGACTATCTTAAAGAAGCCATTGATTTTAGAAGAATTTGGAAAGTCGATCAGAGACCAAAACCAAACATCTTGTGTAAGAGACAGAGATGCATTCTTGAGTAAGGTTTATTCAATTATCTACAACCTTGCAAGAAATGGAGCAACCATGGCTGGAGGTCTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCGTATTACGATGGGTACGAGATCGTTCTTTCCCAGAATCCTTCAACAACCGCCATTATAACACATCAGTCAAACAATATGGCTGCACTAAATACTAGAACTCAACACCACCTCTGA

mRNA sequence

ATGCATGGTAATCAAAGCAAAATGATGAGGTGGTTGATATGGGCACTTTTCTTCCTCATAAGTACCCTTTCCTCTTCATCTTCTCCGGCACCGGCGGCTTTCATCGGAGTTCAAGACGGCCATTTTCAGCTAAAAGGTTCTCCATTTTTGTTCAATGGCTTTAACTCTTATTGGATGATGTCCGTCGCTACTGACCCGAACCAACGCAACAAAGTAACCCAAGTCTTTCGTGATGCCGCCACTGCCGGCCTCACCGTCTGCCGAACCTGGGCCTTCAACGACGGTGGCTTCCATGCCCTCCAAATCTCTCCCGGCGTTTATGATGAATCAGTTTTTCAGGGTCTTGATTTCGTGATATTTGAAGCAAAAAAGTATGGAATTAGAATGATATTGAGTTTGGTAAATAATTTCAAAGACTACGGTGGAAGAGCAGCGTATGTCCGGTGGGCCGAAGCCGCCGGCGTTCAAGTTCATGACGAAGATGACTTCTACACTAATCAACTTATCAGAACATACTACAAAAACCATGTTCAGAAAGTACTGAGAAGGAAGAACACAATGAATGGTTTGAGATATATGGAAGATCCAACCATTATGGGATGGGAACTTATGAACGAACCACGTTGTCAAGTTGATTCCTCCGGCAACACTGTCAATAGATGGGTAGAGGAGATGGGATCATATGTGAAATCAATTGACAAACAACATATGGTGGGGATTGGAATGGAAGGATTCTATGGAGATTCAAGTCCAAATAAAATCAAAGCCAACCCAGGTAGTTTCAAATTTGGCACTGATTTTATCACCAATAACCTCAACAAAGCAATCGACTTTGCAACAATTCATGTATATCCTGATGCGTGGCTACCAGGGAAGAGTGAAGCCACCAAAATGGCATTCCTTGAAGAATGGATAGCACTTCATTGGACGGACGCAAAGACTATCTTAAAGAAGCCATTGATTTTAGAAGAATTTGGAAAGTCGATCAGAGACCAAAACCAAACATCTTGTGTAAGAGACAGAGATGCATTCTTGAGTAAGGTTTATTCAATTATCTACAACCTTGCAAGAAATGGAGCAACCATGGCTGGAGGTCTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCGTATTACGATGGGTACGAGATCGTTCTTTCCCAGAATCCTTCAACAACCGCCATTATAACACATCAGTCAAACAATATGGCTGCACTAAATACTAGAACTCAACACCACCTCTGA

Coding sequence (CDS)

ATGCATGGTAATCAAAGCAAAATGATGAGGTGGTTGATATGGGCACTTTTCTTCCTCATAAGTACCCTTTCCTCTTCATCTTCTCCGGCACCGGCGGCTTTCATCGGAGTTCAAGACGGCCATTTTCAGCTAAAAGGTTCTCCATTTTTGTTCAATGGCTTTAACTCTTATTGGATGATGTCCGTCGCTACTGACCCGAACCAACGCAACAAAGTAACCCAAGTCTTTCGTGATGCCGCCACTGCCGGCCTCACCGTCTGCCGAACCTGGGCCTTCAACGACGGTGGCTTCCATGCCCTCCAAATCTCTCCCGGCGTTTATGATGAATCAGTTTTTCAGGGTCTTGATTTCGTGATATTTGAAGCAAAAAAGTATGGAATTAGAATGATATTGAGTTTGGTAAATAATTTCAAAGACTACGGTGGAAGAGCAGCGTATGTCCGGTGGGCCGAAGCCGCCGGCGTTCAAGTTCATGACGAAGATGACTTCTACACTAATCAACTTATCAGAACATACTACAAAAACCATGTTCAGAAAGTACTGAGAAGGAAGAACACAATGAATGGTTTGAGATATATGGAAGATCCAACCATTATGGGATGGGAACTTATGAACGAACCACGTTGTCAAGTTGATTCCTCCGGCAACACTGTCAATAGATGGGTAGAGGAGATGGGATCATATGTGAAATCAATTGACAAACAACATATGGTGGGGATTGGAATGGAAGGATTCTATGGAGATTCAAGTCCAAATAAAATCAAAGCCAACCCAGGTAGTTTCAAATTTGGCACTGATTTTATCACCAATAACCTCAACAAAGCAATCGACTTTGCAACAATTCATGTATATCCTGATGCGTGGCTACCAGGGAAGAGTGAAGCCACCAAAATGGCATTCCTTGAAGAATGGATAGCACTTCATTGGACGGACGCAAAGACTATCTTAAAGAAGCCATTGATTTTAGAAGAATTTGGAAAGTCGATCAGAGACCAAAACCAAACATCTTGTGTAAGAGACAGAGATGCATTCTTGAGTAAGGTTTATTCAATTATCTACAACCTTGCAAGAAATGGAGCAACCATGGCTGGAGGTCTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCGTATTACGATGGGTACGAGATCGTTCTTTCCCAGAATCCTTCAACAACCGCCATTATAACACATCAGTCAAACAATATGGCTGCACTAAATACTAGAACTCAACACCACCTCTGA

Protein sequence

MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL*
Homology
BLAST of Chy9G157810 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 494.2 bits (1271), Expect = 1.4e-138
Identity = 228/373 (61.13%), Postives = 290/373 (77.75%), Query Frame = 0

Query: 34  FIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFN 93
           F+ V   HF+L GSPFLFNGFNSYW+M VA +P++R KV++V R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 94  DGGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAA 153
           DGG  ALQISPGVYDE VFQGLDFVI EAKKYGIR+ILS VNN+ D+GG+A YV+WA  A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 154 GVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDS 213
           G Q++ +DDFYTN + + YYKNH++KV+ R NT+ G+ Y +D TIM WELMNEPR Q D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 214 SGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLN 273
           SGNT+N WV+EM S+VKS+D +H++ IGMEGFYGDS P +   NPG ++ GTDFI+N+L 
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINPG-YQVGTDFISNHLI 278

Query: 274 KAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIRDQN 333
           K IDFATIH Y D WL G+S+  +M F+++W+  HW DAK ILKKPL+L EFGKS RD  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 334 QTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTT 393
               +  RD F+S +Y  IY+LA++G TM G L+WQ++A+GME+Y DGY I L +NPST 
Sbjct: 339 YNQNI--RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPSTA 398

Query: 394 AIITHQSNNMAAL 407
            IIT QS+ M AL
Sbjct: 399 GIITSQSHAMTAL 408

BLAST of Chy9G157810 vs. ExPASy Swiss-Prot
Match: Q6YM50 (Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.4e-138
Identity = 228/373 (61.13%), Postives = 290/373 (77.75%), Query Frame = 0

Query: 34  FIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFN 93
           F+ V   HF+L GSPFLFNGFNSYW+M VA +P++R KV++V R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 94  DGGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAA 153
           DGG  ALQISPGVYDE VFQGLDFVI EAKKYGIR+ILS VNN+ D+GG+A YV+WA  A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 154 GVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDS 213
           G Q++ +DDFYTN + + YYKNH++KV+ R NT+ G+ Y +D TIM WELMNEPR Q D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 214 SGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLN 273
           SGNT+N WV+EM S+VKS+D +H++ IGMEGFYGDS P +   NPG ++ GTDFI+N+L 
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINPG-YQVGTDFISNHLI 278

Query: 274 KAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIRDQN 333
           K IDFATIH Y D WL G+S+  +M F+++W+  HW DAK ILKKPL+L EFGKS RD  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 334 QTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTT 393
               +  RD F+S +Y  IY+LA++G TM G L+WQ++A+GME+Y DGY I L +NPST 
Sbjct: 339 YNQNI--RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPSTA 398

Query: 394 AIITHQSNNMAAL 407
            IIT QS+ M AL
Sbjct: 399 GIITSQSHAMTAL 408

BLAST of Chy9G157810 vs. ExPASy Swiss-Prot
Match: O48540 (Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 SV=2)

HSP 1 Score: 488.4 bits (1256), Expect = 7.9e-137
Identity = 234/397 (58.94%), Postives = 294/397 (74.06%), Query Frame = 0

Query: 10  RWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQR 69
           R  I  LF L   L      A + FIGV+D HF+L GSPFLFNGFNSYW+M VA DP +R
Sbjct: 6   RSCICGLFLLFLALVCE---ANSGFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADPTER 65

Query: 70  NKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRM 129
            KVT+V +DA+ AGL+VCRTWAF+DGG  ALQISPG+YDE VFQGLDFVI EAKKYGIR+
Sbjct: 66  YKVTEVLKDASVAGLSVCRTWAFSDGGDRALQISPGIYDERVFQGLDFVIAEAKKYGIRL 125

Query: 130 ILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNG 189
           ILS VN + D+GG+A YV WA  AG Q+ ++D+FYT+ +++ Y KNH++KV+ R N++  
Sbjct: 126 ILSFVNQWNDFGGKAQYVWWARNAGAQISNDDEFYTHPMLKKYLKNHIEKVVTRLNSITK 185

Query: 190 LRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDS 249
           + Y +DPTIM WELMNEPR Q D SG TVN WV+EM S+VKS+D +H++ +GMEGFYGDS
Sbjct: 186 VAYKDDPTIMAWELMNEPRDQADYSGKTVNGWVQEMASFVKSLDNKHLLEVGMEGFYGDS 245

Query: 250 SPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHW 309
            P +   NPG ++ GTDFI+N+L   IDFATIH Y D W+ G+S+  ++ ++E+WI  HW
Sbjct: 246 IPERKSVNPG-YQVGTDFISNHLINEIDFATIHAYTDQWVSGQSDDAQLVWMEKWITSHW 305

Query: 310 TDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQ 369
            DA+ ILKKPL+L EFGKS R Q        RD F+S VY  +YNLA+ G TMAG LVWQ
Sbjct: 306 EDARNILKKPLVLAEFGKSSRGQG------SRDIFMSSVYRNVYNLAKEGGTMAGSLVWQ 365

Query: 370 VMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAAL 407
           +MA GME+Y DGY IVL Q PSTT II+ Q++ M AL
Sbjct: 366 LMAHGMENYDDGYCIVLGQTPSTTQIISDQAHVMTAL 392

BLAST of Chy9G157810 vs. ExPASy Swiss-Prot
Match: Q9FUQ6 (Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 3.7e-134
Identity = 237/404 (58.66%), Postives = 298/404 (73.76%), Query Frame = 0

Query: 10  RWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQR 69
           R  I  LF L+  LS     A + FIGV+D HF+L GSPFLFNGFNSYW+M VA DP +R
Sbjct: 7   RSCISGLFLLLLALSCE---ANSGFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADPAER 66

Query: 70  NKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRM 129
            KVT+V +DA+TAGL+VCRTWAF+DGG  ALQISPGVYDE VFQGLDFVI EAKKYG+R+
Sbjct: 67  YKVTEVLKDASTAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVIAEAKKYGVRL 126

Query: 130 ILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNG 189
           ILS VN + D+GG+A YV WA  AGVQ+ ++D+FYT+ +++ Y KNH++ V+ R N++  
Sbjct: 127 ILSFVNQWNDFGGKAEYVWWARNAGVQISNDDEFYTHPILKKYLKNHIE-VVTRLNSITK 186

Query: 190 LRYMEDPTIMGWELMNEPRCQVDSSGNTVN-RWVEEMGSYVKSIDKQHMVGIGMEGFYGD 249
           + Y +D TIM WELMNEPR Q D SG TVN  WV+EM S+VKS+D +H++ +GMEGFYGD
Sbjct: 187 VAYKDDATIMAWELMNEPRDQADYSGKTVNVGWVQEMASFVKSLDNKHLLEVGMEGFYGD 246

Query: 250 SSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALH 309
           S P +   NPG ++ GTDFI+N+L   IDFATIH Y D WL G+S+  ++A++E+WI  H
Sbjct: 247 SIPERKLVNPG-YQVGTDFISNHLINEIDFATIHAYTDQWLSGQSDEAQLAWMEKWIRSH 306

Query: 310 WTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVW 369
           W DA+ ILKKPL+L EFGKS R     S    RD F+S VY  +YNLA+ G TM G LVW
Sbjct: 307 WEDARNILKKPLVLAEFGKSSR-----SGEGSRDIFMSSVYRNVYNLAKEGGTMGGSLVW 366

Query: 370 QVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQH 413
           Q+MA GME+Y DGY IVL  NPSTT II++Q++ M AL     H
Sbjct: 367 QLMAHGMENYDDGYSIVLGLNPSTTQIISNQAHIMTALAHSLNH 400

BLAST of Chy9G157810 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 423.7 bits (1088), Expect = 2.4e-117
Identity = 196/376 (52.13%), Postives = 264/376 (70.21%), Query Frame = 0

Query: 34  FIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFN 93
           F+  Q   F L G+P+  NGFN+YW+M +A DP+QR KV+    +AA  GLTV RTWAF+
Sbjct: 37  FVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALGEAAGHGLTVARTWAFS 96

Query: 94  D-GGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEA 153
           D GG +ALQ+SPG Y+E+ F+GLDFV+ EA+KYGI++ILSLV+N+  +GGR  YV WA A
Sbjct: 97  DGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDNYDSFGGRKQYVNWARA 156

Query: 154 AGVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVD 213
            G  +  +D+F+TN +++ +YKNHV+ VL RKNT+ G+ Y +DPTI+ WELMNEPRCQ D
Sbjct: 157 QGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITGVAYRDDPTILAWELMNEPRCQSD 216

Query: 214 SSGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDSSPNKIKA-NPGSFKFGTDFITNN 273
            SG TV  W+ EM ++VKSID+ HM+ +G+EGFYG SSP++I A NP  ++ GTDFI NN
Sbjct: 217 LSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGASSPSRIAAVNPSGYQLGTDFIANN 276

Query: 274 LNKAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIRD 333
               IDFAT+H YPD WL GK +  ++ F+  W+  H  DA+ +L+KPL++ EFGKS +D
Sbjct: 277 QVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAHIADAQAVLRKPLLIAEFGKSWKD 336

Query: 334 QNQTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNPS 393
              +S    RDA    VY+ IY  AR G    GGL WQ++  GM+SY DGYE+V  + PS
Sbjct: 337 PGYSS--GQRDALYGTVYAKIYESARRGGATVGGLFWQLLVPGMDSYRDGYEVVFGETPS 396

Query: 394 TTAIITHQSNNMAALN 408
           TT +IT  S  +  L+
Sbjct: 397 TTGVITTNSRRLRFLS 410

BLAST of Chy9G157810 vs. ExPASy TrEMBL
Match: A0A0A0KSS2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.1e-233
Identity = 398/416 (95.67%), Postives = 404/416 (97.12%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLS-SSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWM 60
           MHGNQSKMMRWLIWALFFLISTLS SSSSPAPAAF+GVQDGHFQL GSPFLFNGFNSYWM
Sbjct: 1   MHGNQSKMMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWM 60

Query: 61  MSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVI 120
           MSVATDPNQRNKVTQVFRDAA AGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVI
Sbjct: 61  MSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVI 120

Query: 121 FEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQK 180
           FEA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLI+TYYKNHVQK
Sbjct: 121 FEARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQK 180

Query: 181 VLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVG 240
           VL RKNTMNGL YMED TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQH+VG
Sbjct: 181 VLSRKNTMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVG 240

Query: 241 IGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMA 300
           IGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIH YPDAWLPGKSE TKMA
Sbjct: 241 IGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMA 300

Query: 301 FLEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQT-SCVRDRDAFLSKVYSIIYNLARN 360
           FLEEWIALHWTD+KTILKKPLI EEFGKSIRDQNQT S VRDRDAFLSKV+SIIYNLARN
Sbjct: 301 FLEEWIALHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSVRDRDAFLSKVFSIIYNLARN 360

Query: 361 GATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 415
           GATMAGGLVWQVMAEGMESYYDGYEIVLSQ PSTTAIITHQSNNMAALNTRTQHHL
Sbjct: 361 GATMAGGLVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAALNTRTQHHL 416

BLAST of Chy9G157810 vs. ExPASy TrEMBL
Match: A0A5D3BKZ3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00890 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.7e-220
Identity = 372/414 (89.86%), Postives = 390/414 (94.20%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +SKM+RWLIW L  LISTLSSSSSP  A F+GV+DGHFQL GSPFLFNGFNSYWMM
Sbjct: 1   MHG-ESKMIRWLIWGLLLLISTLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KV+QVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF
Sbjct: 61  SVAADPNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLIRTYYKNHVQKV
Sbjct: 121 EARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           LRRKNTM+ LRYM+DPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QH+VGI
Sbjct: 181 LRRKNTMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGFYGDS P KIKANP S+K GTDFITNNLNKAID+ATIH YPDAWLPGKSE TKMAF
Sbjct: 241 GMEGFYGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEW+ALHWTD+KTILKKPLI EEFG+SIRDQNQT  VRDRDAFLSKVYSIIYNLA+NGA
Sbjct: 301 LEEWVALHWTDSKTILKKPLIFEEFGRSIRDQNQTYSVRDRDAFLSKVYSIIYNLAKNGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 415
           TMAGGLVWQVMAEGMESYYDGYEIVLSQNPST AIIT QSN MAAL TRTQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 413

BLAST of Chy9G157810 vs. ExPASy TrEMBL
Match: A0A1S3CB04 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.7e-220
Identity = 372/414 (89.86%), Postives = 390/414 (94.20%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +SKM+RWLIW L  LISTLSSSSSP  A F+GV+DGHFQL GSPFLFNGFNSYWMM
Sbjct: 1   MHG-ESKMIRWLIWGLLLLISTLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KV+QVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF
Sbjct: 61  SVAADPNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLIRTYYKNHVQKV
Sbjct: 121 EARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           LRRKNTM+ LRYM+DPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QH+VGI
Sbjct: 181 LRRKNTMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGFYGDS P KIKANP S+K GTDFITNNLNKAID+ATIH YPDAWLPGKSE TKMAF
Sbjct: 241 GMEGFYGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEW+ALHWTD+KTILKKPLI EEFG+SIRDQNQT  VRDRDAFLSKVYSIIYNLA+NGA
Sbjct: 301 LEEWVALHWTDSKTILKKPLIFEEFGRSIRDQNQTYSVRDRDAFLSKVYSIIYNLAKNGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 415
           TMAGGLVWQVMAEGMESYYDGYEIVLSQNPST AIIT QSN MAAL TRTQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 413

BLAST of Chy9G157810 vs. ExPASy TrEMBL
Match: A0A5A7TCI2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00910 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.2e-197
Identity = 344/414 (83.09%), Postives = 362/414 (87.44%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +SKM+RWLIW L  LISTLSSSSSP  A F+GV+DGHFQL GSPFLFNGFNSYWMM
Sbjct: 1   MHG-ESKMIRWLIWGLLLLISTLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KV+QVFRDAATAGLT                            GLDFVIF
Sbjct: 61  SVAADPNQRHKVSQVFRDAATAGLT----------------------------GLDFVIF 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLIRTYYKNHVQKV
Sbjct: 121 EARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           LRRKNTM+ LRYM+DPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QH+VGI
Sbjct: 181 LRRKNTMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGFYGDS P KIKANP S+K GTDFITNNLNKAID+ATIH YPDAWLPGKSE TKMAF
Sbjct: 241 GMEGFYGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEW+ALHWTD+KTILKKPLI EEFG+SIRDQNQT  VRDRDAFLSKVYSIIYNLA+NGA
Sbjct: 301 LEEWVALHWTDSKTILKKPLIFEEFGRSIRDQNQTYSVRDRDAFLSKVYSIIYNLAKNGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 415
           TMAGGLVWQVMAEGMESYYDGYEIVLSQNPST AIIT QSN MAAL TRTQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 385

BLAST of Chy9G157810 vs. ExPASy TrEMBL
Match: A0A6J1CK63 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.8e-185
Identity = 309/403 (76.67%), Postives = 351/403 (87.10%), Query Frame = 0

Query: 9   MRWLIWALFFLI---STLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMMSVATD 68
           MRWL+W +  ++    + SSS +PAPAAF+G+ DGHFQL GSPF+FNGFNSYWMMSV  D
Sbjct: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60

Query: 69  PNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAKKY 128
           PNQR KV+  FRDAA AGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EA+KY
Sbjct: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120

Query: 129 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKN 188
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+H++DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180

Query: 189 TMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGF 248
           TM+GLRYM+DPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSIDK+H+VGIGMEGF
Sbjct: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240

Query: 249 YGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAFLEEWI 308
           YGDS+P++IK NP  +K GTDF+TNNL K IDFATIH YPD+WLPG+SEATKMAFLEEW+
Sbjct: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300

Query: 309 ALHWTDAKTILKKPLILEEFGKSIRDQNQTSC--VRDRDAFLSKVYSIIYNLARNGATMA 368
            +HW+D+KTILKKPLI  EFGKS ++QNQ     VRDRD+FL+KVYS IYNLARNG TMA
Sbjct: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360

Query: 369 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAAL 407
           GGLVWQVMAEGMESYYDGYEIVLSQNP TTAII  QS NMAAL
Sbjct: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 403

BLAST of Chy9G157810 vs. NCBI nr
Match: XP_004141536.2 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical protein Csa_008142 [Cucumis sativus])

HSP 1 Score: 812 bits (2098), Expect = 1.81e-296
Identity = 398/416 (95.67%), Postives = 404/416 (97.12%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLS-SSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWM 60
           MHGNQSKMMRWLIWALFFLISTLS SSSSPAPAAF+GVQDGHFQL GSPFLFNGFNSYWM
Sbjct: 1   MHGNQSKMMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWM 60

Query: 61  MSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVI 120
           MSVATDPNQRNKVTQVFRDAA AGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVI
Sbjct: 61  MSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVI 120

Query: 121 FEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQK 180
           FEA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLI+TYYKNHVQK
Sbjct: 121 FEARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQK 180

Query: 181 VLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVG 240
           VL RKNTMNGL YMED TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQH+VG
Sbjct: 181 VLSRKNTMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVG 240

Query: 241 IGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMA 300
           IGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIH YPDAWLPGKSE TKMA
Sbjct: 241 IGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMA 300

Query: 301 FLEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSC-VRDRDAFLSKVYSIIYNLARN 360
           FLEEWIALHWTD+KTILKKPLI EEFGKSIRDQNQTS  VRDRDAFLSKV+SIIYNLARN
Sbjct: 301 FLEEWIALHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSVRDRDAFLSKVFSIIYNLARN 360

Query: 361 GATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 414
           GATMAGGLVWQVMAEGMESYYDGYEIVLSQ PSTTAIITHQSNNMAALNTRTQHHL
Sbjct: 361 GATMAGGLVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAALNTRTQHHL 416

BLAST of Chy9G157810 vs. NCBI nr
Match: XP_004141631.3 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KAE8648995.1 hypothetical protein Csa_008431 [Cucumis sativus])

HSP 1 Score: 771 bits (1992), Expect = 2.68e-280
Identity = 374/414 (90.34%), Postives = 391/414 (94.44%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +SKMMRW IW +FFLISTLSSSSSPAPAAF+GVQD HFQL GSPFLFNGFNSYWMM
Sbjct: 1   MHG-ESKMMRWSIWGIFFLISTLSSSSSPAPAAFVGVQDDHFQLNGSPFLFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KVTQVFRDAATAGLTVCRTWAFNDG  HALQISPGVYDESVFQ LDFVIF
Sbjct: 61  SVAADPNQRHKVTQVFRDAATAGLTVCRTWAFNDGALHALQISPGVYDESVFQALDFVIF 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EA+KYGIRM+LSLVNNF++YGGRAAYVRWAEAAGVQVH +DDFYTNQLIRTYYKN+V+KV
Sbjct: 121 EARKYGIRMLLSLVNNFEEYGGRAAYVRWAEAAGVQVHSDDDFYTNQLIRTYYKNYVKKV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           LRRKNTMNGLRYMED TIMGWELMNE RCQVDSSGNT+NRWVEEMGSYVKSIDKQH+VGI
Sbjct: 181 LRRKNTMNGLRYMEDATIMGWELMNEARCQVDSSGNTINRWVEEMGSYVKSIDKQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGFYGDSSPNKIKANPGSFKFGTDF+TNNLNKAIDFATIHVYPDAWLPGKSEAT+MAF
Sbjct: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFVTNNLNKAIDFATIHVYPDAWLPGKSEATRMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEW+ALHW D+K ILKKPLILEEFGKSIR QNQT  VRD DAFLSKVYSIIYNLAR GA
Sbjct: 301 LEEWMALHWMDSKNILKKPLILEEFGKSIRGQNQTFSVRDSDAFLSKVYSIIYNLARKGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 414
           TMAGGLVWQVMAEGMESYYDGYEIVLSQNPST  IIT QSN MAALNTRTQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTNTIITKQSNKMAALNTRTQHHL 413

BLAST of Chy9G157810 vs. NCBI nr
Match: XP_008459570.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 769 bits (1985), Expect = 3.13e-279
Identity = 372/414 (89.86%), Postives = 390/414 (94.20%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +SKM+RWLIW L  LISTLSSSSSP  A F+GV+DGHFQL GSPFLFNGFNSYWMM
Sbjct: 1   MHG-ESKMIRWLIWGLLLLISTLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KV+QVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF
Sbjct: 61  SVAADPNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLIRTYYKNHVQKV
Sbjct: 121 EARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           LRRKNTM+ LRYM+DPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QH+VGI
Sbjct: 181 LRRKNTMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGFYGDS P KIKANP S+K GTDFITNNLNKAID+ATIH YPDAWLPGKSE TKMAF
Sbjct: 241 GMEGFYGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEW+ALHWTD+KTILKKPLI EEFG+SIRDQNQT  VRDRDAFLSKVYSIIYNLA+NGA
Sbjct: 301 LEEWVALHWTDSKTILKKPLIFEEFGRSIRDQNQTYSVRDRDAFLSKVYSIIYNLAKNGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 414
           TMAGGLVWQVMAEGMESYYDGYEIVLSQNPST AIIT QSN MAAL TRTQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 413

BLAST of Chy9G157810 vs. NCBI nr
Match: XP_038890634.1 (mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida])

HSP 1 Score: 718 bits (1853), Expect = 3.89e-259
Identity = 343/414 (82.85%), Postives = 376/414 (90.82%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +   MRWLIW +FF++S LSSS SPAP AF+GVQDGHFQL GSPF+FNGFNSYWMM
Sbjct: 1   MHGERK--MRWLIWEVFFILSGLSSSPSPAPEAFVGVQDGHFQLNGSPFVFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KV+ VF DAA+A LTVCRTWAF DGG  ALQISPGVYDE VFQGLDFVI 
Sbjct: 61  SVAADPNQRHKVSNVFHDAASAALTVCRTWAFADGGSPALQISPGVYDEIVFQGLDFVIC 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EAKKYGIRMILSLVNN+KDYGGR  YVRWA+AAGV ++++DDFYTNQLI++YYKNHVQ+V
Sbjct: 121 EAKKYGIRMILSLVNNYKDYGGREQYVRWAKAAGVDIYNDDDFYTNQLIKSYYKNHVQRV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           L RKNT++GLRYM+DPTIMGWELMNEPRCQ+DSSGNT+NRWVEEMGSYVKSIDKQH+VGI
Sbjct: 181 LTRKNTISGLRYMDDPTIMGWELMNEPRCQLDSSGNTINRWVEEMGSYVKSIDKQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGF GDSSP KIK+NP S++ GTDF+TNNLNK IDFATIH YPDAWLPG+SEATKMAF
Sbjct: 241 GMEGFCGDSSPTKIKSNPNSYRLGTDFLTNNLNKDIDFATIHAYPDAWLPGQSEATKMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEWI +HWTD+KTILKKPLI EEFGKS R QNQTS VRDRD+FLSKVYSIIYNLARNGA
Sbjct: 301 LEEWITIHWTDSKTILKKPLIFEEFGKSTRGQNQTSNVRDRDSFLSKVYSIIYNLARNGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 414
           TMAGGLVWQVMAEGMESYYDGYEIVL QNPST AIIT QSN MAALN +TQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLPQNPSTNAIITQQSNKMAALNIKTQHHL 412

BLAST of Chy9G157810 vs. NCBI nr
Match: KAA0039297.1 (mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 693 bits (1788), Expect = 1.13e-249
Identity = 344/414 (83.09%), Postives = 362/414 (87.44%), Query Frame = 0

Query: 1   MHGNQSKMMRWLIWALFFLISTLSSSSSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMM 60
           MHG +SKM+RWLIW L  LISTLSSSSSP  A F+GV+DGHFQL GSPFLFNGFNSYWMM
Sbjct: 1   MHG-ESKMIRWLIWGLLLLISTLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMM 60

Query: 61  SVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIF 120
           SVA DPNQR+KV+QVFRDAATAGLT                            GLDFVIF
Sbjct: 61  SVAADPNQRHKVSQVFRDAATAGLT----------------------------GLDFVIF 120

Query: 121 EAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKV 180
           EA+KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLIRTYYKNHVQKV
Sbjct: 121 EARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKV 180

Query: 181 LRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGI 240
           LRRKNTM+ LRYM+DPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QH+VGI
Sbjct: 181 LRRKNTMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGI 240

Query: 241 GMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAF 300
           GMEGFYGDS P KIKANP S+K GTDFITNNLNKAID+ATIH YPDAWLPGKSE TKMAF
Sbjct: 241 GMEGFYGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAF 300

Query: 301 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 360
           LEEW+ALHWTD+KTILKKPLI EEFG+SIRDQNQT  VRDRDAFLSKVYSIIYNLA+NGA
Sbjct: 301 LEEWVALHWTDSKTILKKPLIFEEFGRSIRDQNQTYSVRDRDAFLSKVYSIIYNLAKNGA 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAALNTRTQHHL 414
           TMAGGLVWQVMAEGMESYYDGYEIVLSQNPST AIIT QSN MAAL TRTQHHL
Sbjct: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 385

BLAST of Chy9G157810 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.6e-113
Identity = 184/373 (49.33%), Postives = 253/373 (67.83%), Query Frame = 0

Query: 34  FIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFN 93
           F+  +   F L G P+  NGFN+YW+M VA+DP+QR+K++  F+DA+  GLTV RTWAF+
Sbjct: 31  FVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFS 90

Query: 94  DGGFHALQISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAA 153
           DGG+ ALQ SPG Y+E +FQGLDF + EA+++GI++ILS  NN++ +GGR  YV WA + 
Sbjct: 91  DGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSR 150

Query: 154 GVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDS 213
           G  V  EDDF+T+ L++ +YKNH++ VL R NT   + Y +DPTIM WELMNEPRC  D 
Sbjct: 151 GRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDP 210

Query: 214 SGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLN 273
           SG  +  W+ EM ++VKS+D+ H++  G+EGFYG SSP     NP   +FGTDFI NN  
Sbjct: 211 SGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPG-QFGTDFIANNRI 270

Query: 274 KAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIRDQN 333
             IDF T+H YPD W P  SE ++M FL +W+  H  DA+ +L KP+IL EFGKS++   
Sbjct: 271 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 330

Query: 334 QTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTT 393
            T     RD   + VYS IY  A+ G   AGGL WQ++  G++++ DGY I+LSQ+ ST 
Sbjct: 331 YTPA--QRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTV 390

Query: 394 AIITHQSNNMAAL 407
            +I+ QS  +  +
Sbjct: 391 NVISQQSRKLTLI 400

BLAST of Chy9G157810 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 389.8 bits (1000), Expect = 2.7e-108
Identity = 191/405 (47.16%), Postives = 252/405 (62.22%), Query Frame = 0

Query: 9   MRWLIWALFFLISTLSSSS-----SPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMMSVA 68
           M+ L + +   I    +SS     S +   F+  +   F L G PF  NGFN+YW+   A
Sbjct: 1   MKCLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEA 60

Query: 69  TDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAK 128
           TD   R K+T VF++A    LT+ RTW F DGG+ ALQI+PGVYDE  FQGLDF I EAK
Sbjct: 61  TDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAK 120

Query: 129 KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKVLRR 188
           + GI+MI++ VNN+ D+GGR  YV WA+  G  V  +DDFYTN L++ YYKNHV+ ++ R
Sbjct: 121 RLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNR 180

Query: 189 KNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGIGME 248
            NT   + Y ++PTIMGWELMNEP+C+ D SG T+  W+ EM  YVKS+D +H++  G+E
Sbjct: 181 VNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLE 240

Query: 249 GFYGDSSP-NKIKANP-GSFKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEATKMAFL 308
           GFYGDSSP  K   NP  +   GTDFI N+   AIDFA+IH YPD W P   E +++  L
Sbjct: 241 GFYGDSSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 300

Query: 309 EEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGAT 368
            +W+  H  DA+ ILKKPLIL EFGK       T     RDA  +  +  IY  A  G  
Sbjct: 301 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQA--QRDAVFNATFDTIYESAEKGGP 360

Query: 369 MAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQSNNMAAL 407
            AG L W V+++GM ++ D   IVLS+N +T  IIT +S  +  +
Sbjct: 361 AAGALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLI 403

BLAST of Chy9G157810 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 7.7e-103
Identity = 177/369 (47.97%), Postives = 240/369 (65.04%), Query Frame = 0

Query: 42  FQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFNDGGFHALQ 101
           F L G PF  NGFN+YWMM++A D + R KVT+VF+ A+  G+TV RTWAFNDG + ALQ
Sbjct: 55  FTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQ 114

Query: 102 ISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDED 161
            SP VYDE VF+ LDFV+ EA+KY IR+ILSLVNN+  YGG+A YV+W  A+G+ +  +D
Sbjct: 115 KSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASGLNLTSDD 174

Query: 162 DFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRW 221
           DF+TN  +R +Y++HV+ VL R NT   + Y  DPTI  WELMNEPRC  D SG+ +  W
Sbjct: 175 DFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSW 234

Query: 222 VEEMGSYVKSIDKQHMVGIGMEGFYGDSSPNKIKANPGSF--KFGTDFITNNLNKAIDFA 281
           ++EM  +VKS+D +H+V IG+EGFYG S+P + + NP  +  + GTDFI NN    IDFA
Sbjct: 235 IQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFA 294

Query: 282 TIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVR 341
           ++HVYPD+W+      + + F   W+  H  DA+  L  P++  EFG S  D    +   
Sbjct: 295 SVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSF- 354

Query: 342 DRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITHQ 401
            RD  L+ VY +  N  R G   AG LVWQV  +G E   DGY + L++  + + II+ Q
Sbjct: 355 -RDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASKIISLQ 414

Query: 402 SNNMAALNT 409
           S  +A  N+
Sbjct: 415 SKRLAIFNS 421

BLAST of Chy9G157810 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 6.1e-100
Identity = 184/407 (45.21%), Postives = 251/407 (61.67%), Query Frame = 0

Query: 8   MMRWLIWALFFLISTLSSS--SSPAPAAFIGVQDGHFQLKGSPFLFNGFNSYWMMSVATD 67
           M+  L + LFFL   + ++         F+G     F L G     NGFN+YWMM+ A D
Sbjct: 1   MLNILPFFLFFLPFLIGNNRICVAVKTGFVGRNGTQFVLNGEQVYLNGFNAYWMMTTAAD 60

Query: 68  PNQRNK--VTQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAK 127
              + +  VT   R A+  G+ V R W FN+G +  LQISPG Y E VF+GLDFV++EA 
Sbjct: 61  TASKGRATVTTALRQASAVGMNVARIWGFNEGDYIPLQISPGSYSEDVFKGLDFVVYEAG 120

Query: 128 KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIRTYYKNHVQKVLRR 187
           ++ I++I+SLVNNF+DYGGR  YV WA      + + D+FYTN  ++ +YKNHV+ VL R
Sbjct: 121 RFNIKLIISLVNNFEDYGGRKKYVEWA-----GLDEPDEFYTNSAVKQFYKNHVKTVLTR 180

Query: 188 KNTMNGLRYMEDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHMVGIGME 247
           KNT+ G  Y +DPTI  WEL+NEPRC   ++ N +  WV+EM SYVKSID  H++ IG+E
Sbjct: 181 KNTITGRMYKDDPTIFSWELINEPRCNDSTASNILQDWVKEMASYVKSIDSNHLLEIGLE 240

Query: 248 GFYGDSSPNKIKANPGS-FKFGTDFITNNLNKAIDFATIHVYPDAWLPGKSEAT--KMAF 307
           GFYG+S P +   NPG     GTDFITNN    IDFATIH+YPD+WLP +S  T  +  F
Sbjct: 241 GFYGESIPERTVYNPGGRVLTGTDFITNNQIPDIDFATIHIYPDSWLPLQSSRTGEQDTF 300

Query: 308 LEEWIALHWTDAKTILKKPLILEEFGKSIRDQNQTSCVRDRDAFLSKVYSIIYNLARNGA 367
           ++ WI  H  D   I+KKPL++ EFGKS +    +  +  R+ F  +VY +IY+ AR G 
Sbjct: 301 VDRWIGAHIEDCDNIIKKPLLITEFGKSSKYPGFS--LEKRNKFFQRVYDVIYDSARAGG 360

Query: 368 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTA-IITHQSNNMAAL 407
           +  GG+ WQ+         DGYE+ +   P+TTA +I  QS+ +  L
Sbjct: 361 SCTGGVFWQLTTNRTGLLGDGYEVFMQAGPNTTAQLIADQSSKLKNL 400

BLAST of Chy9G157810 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 3.3e-98
Identity = 178/381 (46.72%), Postives = 235/381 (61.68%), Query Frame = 0

Query: 34  FIGVQDGHFQLKGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAATAGLTVCRTWAFN 93
           F+      F L G PF  NGFN+YW+   ATDP  R K+T VF++A + GLT+ RTW F 
Sbjct: 29  FVSRNGVQFILNGKPFYANGFNAYWLAYEATDPTTRFKITNVFQNATSLGLTIARTWGFR 88

Query: 94  DGG-FHALQISPGVYDESVFQGLDFVIFEAKKYGIRMILSLVNNFKDYGGRAAYVRWAEA 153
           DG  + ALQ +PG YDE  FQGLDFVI EAK+ GI++I+ LVNN+ DYGG+  YV WA +
Sbjct: 89  DGAIYRALQTAPGSYDEQTFQGLDFVIAEAKRIGIKLIILLVNNWDDYGGKKQYVDWARS 148

Query: 154 AGVQVHDEDDFYTNQLIRTYYKNHVQKVLRRKNTMNGLRYMEDPTIMGWELMNEPRCQVD 213
            G  V   DDFY N +I+ +YKNHV+ VL R NT   + Y ++P IM W+LMNEPRC VD
Sbjct: 149 KGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRVNTFTKVAYKDEPAIMAWQLMNEPRCGVD 208

Query: 214 SSGNTVNRWVEEMGSYVKSIDKQHMVGIGMEGFYGDSSP-NKIKANPGSFK-FGTDFITN 273
            SG T+  W+ EM  +VKS+D  H++  G EGFYGDSSP  K   NP S    G DFI N
Sbjct: 209 KSGKTLMDWINEMAPFVKSVDPNHLLSTGHEGFYGDSSPERKNSLNPVSANTVGADFIAN 268

Query: 274 NLNKAIDFATIHVYPDAWLPGKSEATKMAFLEEWIALHWTDAKTILKKPLILEEFGKSIR 333
           +   AIDFA++H   D W     + +++AF++ W+  H  DA+ ILKKP+IL EFG    
Sbjct: 269 HNIDAIDFASMHCGSDLWFQRLDQNSRLAFIKRWLEGHIEDAQNILKKPVILAEFGLGSD 328

Query: 334 DQNQTSCVRDRDAFLSKVYSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQNP 393
               T  + +RD   +  Y IIY  A+ G + AG L W+V++EGM ++     I+LS   
Sbjct: 329 TPRYT--LANRDGVFTTTYDIIYASAQKGGSAAGALFWEVISEGMSNFAGPSSIILSDKS 388

Query: 394 STTAIITHQSNNMAALNTRTQ 412
           ST  II+  +  M  L   T+
Sbjct: 389 STVNIISEHARKMGLLGETTR 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ031.4e-13861.13Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 ... [more]
Q6YM501.4e-13861.13Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 ... [more]
O485407.9e-13758.94Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 ... [more]
Q9FUQ63.7e-13458.66Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 ... [more]
Q0JKM92.4e-11752.13Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Match NameE-valueIdentityDescription
A0A0A0KSS22.1e-23395.67Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3... [more]
A0A5D3BKZ32.7e-22089.86Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CB042.7e-22089.86Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV... [more]
A0A5A7TCI23.2e-19783.09Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1CK632.8e-18576.67Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 ... [more]
Match NameE-valueIdentityDescription
XP_004141536.21.81e-29695.67mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical pr... [more]
XP_004141631.32.68e-28090.34mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo... [more]
XP_008459570.13.13e-27989.86PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 ma... [more]
XP_038890634.13.89e-25982.85mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida][more]
KAA0039297.11.13e-24983.09mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G66460.11.6e-11349.33Glycosyl hydrolase superfamily protein [more]
AT3G10890.12.7e-10847.16Glycosyl hydrolase superfamily protein [more]
AT5G01930.17.7e-10347.97Glycosyl hydrolase superfamily protein [more]
AT1G02310.16.1e-10045.21Glycosyl hydrolase superfamily protein [more]
AT3G10900.13.3e-9846.72Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 31..407
e-value: 1.6E-126
score: 424.2
NoneNo IPR availablePANTHERPTHR31451:SF53MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEINcoord: 6..412
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 45..344
e-value: 5.8E-17
score: 62.0
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 6..412
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 34..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G157810.1Chy9G157810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds