Chy8G153130 (gene) Cucumber (hystrix) v1

BLAST of Chy8G153130 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 563.1 bits (1450), Expect = 3.5e-159
Identity = 285/537 (53.07%), Postives = 379/537 (70.58%), Query Frame = 0

Query: 17  KETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFA 76
           K+ +GGWRA+++I+ NE+FEKLASMSLIGNL +YL T YNL  V    V  IW G+ N  
Sbjct: 15  KKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNIL 74

Query: 77  PLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGESNCPQPLPWQ 136
            LAGAF++DAYLGRF+TLL GSIAS +GMG+  L A LP +RP  CI   +   QP  WQ
Sbjct: 75  TLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQ 134

Query: 137 LGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIAL 196
           LG L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWYF F++AL+IAL
Sbjct: 135 LGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIAL 194

Query: 197 TIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRK 256
           T VVY+QTNISW +GF IPTAC   SI  F++G   YIC   +GSVFAD+ KVV A C+K
Sbjct: 195 TGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKK 254

Query: 257 CQIPKTTQFDQLHNPPMNSSK---LAHTNRFLIFDKAATVVDSSEELDEEGKSKNEWRLC 316
            ++   +       P  + S    +   +R   FDK A++V +  EL+E+G +K +WRLC
Sbjct: 255 RKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDK-ASIVTNPNELNEDGNAKYKWRLC 314

Query: 317 SVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPH-FQIPPAWMSL 376
           SV QV+  KCV  I+PVW+ GI CF+   Q + +GILQA+QM+++ GPH FQ+P  WM+L
Sbjct: 315 SVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNL 374

Query: 377 TPMIALSIWIYIYE-KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFRRD 436
             MI L+IWI +YE   +  +K+ T   KR ++K RIE  IVM ++CM+VAG  EK RR 
Sbjct: 375 VSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRA 434

Query: 437 AAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRTVAGAIFFLSLS 496
           +A++N SF SP+ + +L+P+FAL+GLTEAF A+A+ME LT  +PE +R VAGAIFFLS S
Sbjct: 435 SALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSS 494

Query: 497 LASYLSSVLTGIVREVERE----WLGGNDLNKNRLDYFFYVVGVIAALNFFYFRFFA 545
           +ASY+ ++L  ++  V R+    WLG  DLNKNRL+ +F+++  I   N  YFR FA
Sbjct: 495 IASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLFA 548

BLAST of Chy8G153130 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 3.1e-115
Identity = 221/576 (38.37%), Postives = 350/576 (60.76%), Query Frame = 0

Query: 9   SSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 68
           S LDA + ++  GGWRAV FILGNET E+L S+ L+ N ++YL  +++L+ V +A V  I
Sbjct: 41  SILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINI 100

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGES- 128
           W G TN  PL GA+++D Y+GRF T+ F S A+LLG+  +TL A  PQ+ P  C S +  
Sbjct: 101 WSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPL 160

Query: 129 NCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFL 188
           +C  P   Q+G L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  
Sbjct: 161 SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMT 220

Query: 189 FSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMA 248
           F++ LII  T+VVY+Q  +SW +GF IPT     ++ +F  G + Y+   P+GS+F+ +A
Sbjct: 221 FTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIA 280

Query: 249 KVVVATCRKCQIPKTTQFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSEELD 308
           +V+VA  +K ++    + D     ++P + S   SKL  +N+F   DKAA V++   +L 
Sbjct: 281 QVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLT 340

Query: 309 EEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGP 368
            EG   ++WRLCSV +VE+ KC++ IVP+W AGI    +M    +F + QA++M+R++GP
Sbjct: 341 PEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGP 400

Query: 369 HFQIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 428
            F+IP   +S+  ++ + I++  Y++ +V  M++ T      ++  RI  GIV ++  M+
Sbjct: 401 KFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMI 460

Query: 429 VAGVLEKFRRDAAVE--NKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESL 488
           VAG++E+ RR  ++   + +  +P+ V+ L P+  L GL EAF  I  +E   +  PE +
Sbjct: 461 VAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHM 520

Query: 489 RTVAGAIFFLSLSLASYLSSVLTGIVREV-----EREWLGGNDLNKNRLDYFFYVVGVIA 548
           R++A ++F LS + +SYLSS L  +V +        +WL  N LN  +LDYF+Y++ V+ 
Sbjct: 521 RSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLG 580

Query: 549 ALNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAEL 570
            +N  YF + A  +  K  +        +  +D E+
Sbjct: 581 VVNLVYFWYCARGYRYKVGLPIEDFEEDKSSDDVEM 613

BLAST of Chy8G153130 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 415.2 bits (1066), Expect = 1.2e-114
Identity = 228/568 (40.14%), Postives = 347/568 (61.09%), Query Frame = 0

Query: 21  GGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFAPLAG 80
           GGWRA+ FILGNET EKL S+ +  N +LYL  +++++ V +  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 81  AFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGE-SNCPQPLPWQLGF 140
           A ++DAY+GRF T+ + S+ S+LG+  +TL A LPQ+ PPPC +     C  P   QLG 
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 141 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTIV 200
           L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ LI + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 201 VYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRKCQI 260
           VY+QT +SW +GF IPT+    ++ +F +G R+Y+   P+GSVF+ +A+V+VA  +K  +
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 261 PKTTQFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSEELDEEGKSKNEWRLC 320
             +   D   + + PP+     SKL  T++F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 321 SVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHFQIPPAWMSLT 380
           S+ +VE+ KC++ +VPVW AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 381 PMIALSIWIYIYE----KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFR 440
             I + IW+ IYE     ++  M+K      R ++  R+ IGIV ++L M  AG +E  R
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRMRK-----FRVTLLQRMGIGIVFAILSMFTAGFVEGVR 435

Query: 441 RDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRTVAGAIFFLS 500
           R  A E    T     W+ +P   L GL E+F  I ++E   +  PE +R++A ++F LS
Sbjct: 436 RTRATE---MTQMSVFWLALP-LILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLS 495

Query: 501 LSLASYLSSVLTGIVREV-----EREWLGGNDLNKNRLDYFFYVVGVIAALNFFYFRFFA 560
            + A+YLSS+L   V +V       +WL   DL++ +LDYF+Y++ V+  +N  YF + A
Sbjct: 496 FAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCA 555

Query: 561 VSFLPKPDVDPRQRAHQRQLEDAELGRR 573
             +  K          ++ L D E  +R
Sbjct: 556 HRYQYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of Chy8G153130 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.7e-101
Identity = 197/545 (36.15%), Postives = 331/545 (60.73%), Query Frame = 0

Query: 9   SSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 68
           SS D + ++    GW+A+ +I+GNET E+LA+  L+ N ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGESN 128
           W   TNFAP+ GAF++D+Y G+F T++FGSIA LLGM VLT  + +P +RPPPC + +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPC-TADQI 127

Query: 129 CPQPLPWQLGFLYL---GLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY 188
             Q +P+    LY+   GL L+ +G GGIR C++ F  DQFD +TE+GR    SF +W+Y
Sbjct: 128 TGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYY 187

Query: 189 FLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFAD 248
              +I  ++++T+V+YVQ NISW +GF IPT    F++ +  +G RYY+   P+GSVF+ 
Sbjct: 188 TTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSG 247

Query: 249 MAKVVVATCRKCQIPKTTQFDQLHNP----PMNSSKLAHTNRFLIFDKAATVVDSSEELD 308
           + KV+VA  +K +   T+  D  H P     + S+KL  T++F   +KA  V+++ E  +
Sbjct: 248 VFKVLVAAYKKRKARFTSGID-YHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNNDEAGN 307

Query: 309 EEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSI-G 368
           E      EWR C+V Q+E  K ++ I+P++ + I  F++M Q  +F + QA++M+    G
Sbjct: 308 E------EWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPG 367

Query: 369 PHFQIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCM 428
             + IPPA +++  ++ + IW+  YE   V H++  T+ N   S+  ++ IG + S+  M
Sbjct: 368 TSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTM 427

Query: 429 VVAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLR 488
           +++G++E+ RRD ++        + V+ L P+  L G  + F  + + E     +P ++R
Sbjct: 428 LISGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMR 487

Query: 489 TVAGAIFFLSLSLASYLSSVLTGIVREVE----REWLGGNDLNKNRLDYFFYVVGVIAAL 541
           ++  ++ +L LSLASYLSS +  IV  V     + WL  +D++K++LD F+Y +  ++ L
Sbjct: 488 SIGNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTL 539

BLAST of Chy8G153130 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.7e-101
Identity = 204/546 (37.36%), Postives = 321/546 (58.79%), Query Frame = 0

Query: 14  SRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 73
           ++KK    GW+ + FI+GNETFEKL  +  + NL++YL  ++NL ++ +A +   + GT 
Sbjct: 38  NQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTI 97

Query: 74  NFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPC-ISGESNCPQP 133
           NF     AFL D Y GR+ TL    IA  LG  V+ L A +PQ+ P  C  + +S C  P
Sbjct: 98  NFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGP 157

Query: 134 LPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIAL 193
              Q+ FL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A 
Sbjct: 158 SGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQ 217

Query: 194 IIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVA 253
           I++LT+VVYVQ+N+SWT+G  IP      +  IF  G + Y+     GS  A +A+V+  
Sbjct: 218 ILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAV 277

Query: 254 TCRKCQI-PKTTQFDQLHN---PPMNSSKLAHTNRFLIFDKAATVVDSSEELDEEGKSKN 313
             +K  + P    +  L+N   P   +SKL +T++F   DKAA ++   ++L  +GK  +
Sbjct: 278 AIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPAD 337

Query: 314 EWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGP-HFQIPP 373
            W+LC++ QVE+ KC+V ++P+W A    ++++ Q  ++ + QA+Q +R +G   F IP 
Sbjct: 338 PWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPA 397

Query: 374 AWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLE 433
           A   +  M  ++++I +Y++  V  M++ T  +   ++  RI  GI  +   +VVAG +E
Sbjct: 398 ATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVE 457

Query: 434 KFRRDAAV--------ENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESL 493
           + RR  A+          K   S +    LIP+ +L+G+ EAF AI  ME      PE++
Sbjct: 458 ERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENM 517

Query: 494 RTVAGAIFFLSLSLASYLSSVLTGIVREVEREWLGGN----DLNKNRLDYFFYVVGVIAA 541
           R+ AG+IF++   ++SYL S L   V    +   GGN    DLNK RLD F++++  I A
Sbjct: 518 RSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILA 577

BLAST of Chy8G153130 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.1e-260
Identity = 457/578 (79.07%), Postives = 504/578 (87.20%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           M+NG  L SSLD S K +  GGWRAVRFIL NETFEKLASMSLI NL+LYLHTMYNLDNV
Sbjct: 1   MDNGT-LRSSLDPSPKPQL-GGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIAS LGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC SG+++C Q   WQL FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWYFLFSIAL+IALT+VVY+QTNISWTLGF IPTA F+FSI IFLLG   YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSEEL 300
           SVFADMAKV++AT RK QIP+ +  + LH+PPM+SSKL HTNRFL+FDKAA V+DSS EL
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSP-NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DE+G+SKNEW+LC+VHQVEQ KCVVGIVP+W AGI CF+SMQQM SFGILQAIQMNR +G
Sbjct: 301 DEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHF+IPPAWMSL PMIALS+WIYIYEKYVE M++KT  NKRFSMK RIEIGIVMSVLCM+
Sbjct: 361 PHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCMI 420

Query: 421 VAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRT 480
           VAG+LEK RRD+A+EN++F SPLHV VLIPEFALSGLTEAF AI++MELLTT LPESLRT
Sbjct: 421 VAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLTGIVREVER------EWLGGNDLNKNRLDYFFYVVGVIAA 540
           VAGAIFFLSLSLASYLSSVL GIV  V +       WL GNDLNKN LDYFFYVVGVIAA
Sbjct: 481 VAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAELGRR 573
           LNFFYFRF    FLP+ DVDPR +A +R+ ED E G R
Sbjct: 541 LNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of Chy8G153130 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.6e-258
Identity = 442/468 (94.44%), Postives = 455/468 (97.22%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGAD 164
           MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQL FLYLGLGLIVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIF 224
           QFDTNTEKGRAKLESFLNWWYFLFS+AL+IALTIVVYVQTN+SWTLGFGIPTACFVFSI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 225 IFLLGHRYYICKAPQGSVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRF 284
           IFLLGHRYYICKAPQGSVFAD+AKVVVATCRK QIPK TQFDQLHNPPMNSSKLAHTNRF
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPMNSSKLAHTNRF 180

Query: 285 LIFDKAATVVDSSEELDEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQM 344
           LIFDKAATVVDSS ELDE+GKSKNEWRLCSVHQVE+FKCVVGI+PVWLAGIPCFMSMQQM
Sbjct: 181 LIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQQM 240

Query: 345 SSFGILQAIQMNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSM 404
            SFGILQAIQMNR IGPHFQIPPAWMSLTPMI LSIWIYIYEKYVEHMKKKT+SNKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRFSM 300

Query: 405 KTRIEIGIVMSVLCMVVAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAI 464
           KTRIEIGIVMSVLCMVVAG+LEK RRDAAVENKSF SPLHVWVLIPEFALSGLTEAF AI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFAAI 360

Query: 465 AIMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVREVEREWLGGNDLNKNRLDY 524
           A+MELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIV +VEREWLGGNDLNKNRLDY
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKVEREWLGGNDLNKNRLDY 420

Query: 525 FFYVVGVIAALNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAELGRR 573
           FFYVVGVIAALNFFYFRFFA +FLPKPDVDPRQ+AHQRQLEDAELGRR
Sbjct: 421 FFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of Chy8G153130 vs. ExPASy TrEMBL
Match: A0A0A0KZQ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297490 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.8e-249
Identity = 455/563 (80.82%), Postives = 460/563 (81.71%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           MENGNLLH+SLDASR KETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGS+ASLLGMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCISGESNCPQPLPWQLGFLYLGLGLIVIGAG                            
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAG---------------------------- 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSEEL 300
               DMAKVVVATCRK QIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSS EL
Sbjct: 241 ----DMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DEEGKSKNEWRLCSVHQVEQFKCVVGI PVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG
Sbjct: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRT 480
           VAGVLEKFRRDAAVENKSF SPLHVWVLIPEFALSGLTEAFGAIA+MELLTTHLPESLRT
Sbjct: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 471

Query: 481 VAGAIFFLSLSLASYLSSVLTGIVREVEREWLGGNDLNKNRLDYFFYVVGVIAALNFFYF 540
           VAGAIFF SLSLASYLSSVLTGIVR VEREWLGGNDLNKNRLDYFF VVGVIAALNFFYF
Sbjct: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF 471

Query: 541 RFFAVSFLPKPDVDPRQRAHQRQ 564
           RFFAV+FLPKPDVDP  +AHQ Q
Sbjct: 541 RFFAVAFLPKPDVDPSHKAHQMQ 471

BLAST of Chy8G153130 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 2.3e-246
Identity = 432/585 (73.85%), Postives = 481/585 (82.22%), Query Frame = 0

Query: 1   MENGNLLHSSLD---ASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNL 60
           MEN  + HSSLD    +  K   GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 61  DNVASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQM 120
           DNVASA V  IWGGTTNFAP+AGAFLADAYLGRFYTLLFGSIAS  GMGVLTLGAGLP++
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 121 RPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180
           RPP C   ++ CPQP PWQL FLYLGLGL+VIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKA 240
           ESFLNWWYFLFSIALI+ALT+VVY+QTNISW  GF IPTACFV SI IFLLG   YI K 
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 241 PQGSVFADMAKVVVATCRKCQIPKTTQFDQLHNPP---MNSSKLAHTNRFLIFDKAATVV 300
           PQGSVFADMAKV++ATCRK ++ K    + LH+PP    +S+KL HTNRFL+FDKAATVV
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 301 DSSEELDEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQ 360
           D   ELDE G S + WRLCSV QVEQ KCV+GIVPVW+AGI CF++MQQM SFG+LQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 361 MNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVM 420
           M+R +G HF+IPPAWM+LTPMIALSIWI++YEKYV  MK+KT  NKR SM+ RIEIGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 421 SVLCMVVAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHL 480
           SV+CMVV+G+ EK RRD AV N SF + LHV  L PEFALSGLTEAF AIA+MELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLTGIVREV-------EREWLGGNDLNKNRLDYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVL GI+ ++       +  W+GGNDLN NRL+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 541 VVGVIAALNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAELGRR 573
           VV VI  LNFFYFRFFA  FLPK DVD   + H+RQLED E GRR
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSDVDDHDQTHERQLEDEETGRR 585

BLAST of Chy8G153130 vs. ExPASy TrEMBL
Match: A0A6J1IS88 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111478233 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.7e-223
Identity = 384/455 (84.40%), Postives = 411/455 (90.33%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGAD 164
           MGVLTLGAG P+MRPPPC  GESNCPQP PWQLGFLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIF 224
           QFDTNTEKGRAKLESFLNWWY LFS+AL+IALT+VVY+QT+ISWTLGF IPTACFVFSI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 225 IFLLGHRYYICKAPQGSVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRF 284
           IFLLG R+YICK PQGSVFADMAKV+VATCRK  +PK TQ D L++PP++SSKLAHT+RF
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 180

Query: 285 LIFDKAATVVDSSEELDEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQM 344
           L+FDKAATV DSS ELDE+GKSKNEW+LCSVHQVEQ KCVVGIVP+W AGIPCF+SMQQM
Sbjct: 181 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 240

Query: 345 SSFGILQAIQMNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSM 404
            SFGILQAIQMNR IGPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT  NKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 300

Query: 405 KTRIEIGIVMSVLCMVVAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAI 464
           KTRIEIGIVMSVLCMVVAG+LE  RR +AVENK F SPLHVW LIPEFALSGLTEAF AI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 360

Query: 465 AIMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVREVER------EWLGGNDLN 524
           A+MELLTTHLPESLRTVAGAIFFLSLSL SY+SSVL  IV ++ R      EWLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 420

Query: 525 KNRLDYFFYVVGVIAALNFFYFRFFAVSFLPKPDV 554
           KNRLDYFFYVVGVIAA NFFYFRFFA  FLPKP+V
Sbjct: 421 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

BLAST of Chy8G153130 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 1111 bits (2874), Expect = 0.0
Identity = 554/572 (96.85%), Postives = 561/572 (98.08%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           MENGNLLH+SLDASR KETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGS+ASLLGMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWYFLFSI+L+IALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG
Sbjct: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSEEL 300
           SVFADMAKVVVATCRK QIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSS EL
Sbjct: 241 SVFADMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DEEGKSKNEWRLCSVHQVEQFKCVVGI PVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG
Sbjct: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRT 480
           VAGVLEKFRRDAAVENKSF SPLHVWVLIPEFALSGLTEAFGAIA+MELLTTHLPESLRT
Sbjct: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLTGIVREVEREWLGGNDLNKNRLDYFFYVVGVIAALNFFYF 540
           VAGAIFF SLSLASYLSSVLTGIVR VEREWLGGNDLNKNRLDYFF VVGVIAALNFFYF
Sbjct: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF 540

Query: 541 RFFAVSFLPKPDVDPRQRAHQRQLEDAELGRR 572
           RFFAV+FLPKPDVDP  +AHQ QLEDAELGRR
Sbjct: 541 RFFAVAFLPKPDVDPSHKAHQMQLEDAELGRR 572

BLAST of Chy8G153130 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1016 bits (2627), Expect = 0.0
Identity = 511/578 (88.41%), Postives = 533/578 (92.21%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           M+N N  HSS DA RKK  RGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MDNENR-HSSSDAPRKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIAS LGMGVL+LGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCISGES+CPQP PWQLGFLYLGLGLIV+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWY LFSIAL+IALTIVVYVQTNISWTLGF IPTACFV SI IFL G R+Y+CKAPQG
Sbjct: 181 LNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQG 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSEEL 300
           SVF+D+AKVVVA  RK +IPK  Q D LHNPPMNS KLAHT+RFL+FDKAATVVDSS EL
Sbjct: 241 SVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRFLVFDKAATVVDSSIEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DEEGKSK+EWRLCSVHQVEQFKCVVGI+PVWLAGI CF+SMQQM SFGILQAIQMNR IG
Sbjct: 301 DEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT SNKRFSMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRT 480
           VAG+LEK RRDAAVENKSF SPLHVW+LIPEFALSGLTEAF AIA+MELLTTHLPESLRT
Sbjct: 421 VAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLTGIVREV------EREWLGGNDLNKNRLDYFFYVVGVIAA 540
           VAGAIFFLSLSLASYLSSVL GIV ++      E +WLGGNDLNKNRLDYFFYVVG IAA
Sbjct: 481 VAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAA 540

Query: 541 LNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAELGRR 572
           LNFFYFRFFA  FLP PDVDPRQ+AHQRQLEDAELGRR
Sbjct: 541 LNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 577

BLAST of Chy8G153130 vs. NCBI nr
Match: XP_023544027.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 943 bits (2437), Expect = 0.0
Identity = 471/559 (84.26%), Postives = 504/559 (90.16%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           ME+G L HSS +  R K   GGWRAVRFILGNETFEKLASMSLI NLVLYLHTMYNLD+V
Sbjct: 1   MESGRL-HSSFN--RPKAALGGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIW GTTNFA LAGAFLADAYLGRFYTLLFGS ASLLGMGVLTLGAG P+MRPP
Sbjct: 61  DSAYVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC  GESNCPQP PWQLGFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWY LFS+AL+IALT+VVYVQT+ISWTLGF IPTACFVFSI IFLLG R+YICK PQG
Sbjct: 181 LNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQG 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSEEL 300
           SVFADMAKV+VATCRK  +PK TQ D LH+PPM+SSKL+HT+RFL+FDKAATVVDSS EL
Sbjct: 241 SVFADMAKVLVATCRKRNVPKFTQIDHLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DE+GKSKNEW+LCSVHQVEQFKCVVGIVP+W+AGIPCF+SMQQM SFGILQAIQMNR IG
Sbjct: 301 DEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWVAGIPCFISMQQMGSFGILQAIQMNRFIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEK+VE MKKK   NKRFSMKTRIEIGIVMSV+CMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKFVEFMKKKNHKNKRFSMKTRIEIGIVMSVICMV 420

Query: 421 VAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRT 480
           VAG+LE  RR++AVENKSF SPLHVW+LIPEFALSGLTEAF AIA+MELLTTHLPESLRT
Sbjct: 421 VAGILENVRRNSAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLTGIVREVERE------WLGGNDLNKNRLDYFFYVVGVIAA 540
           VAGAIFFLSLSL SY+SSVL  IV ++ R       WLGGNDLNKNRLDYFFYVVGVIAA
Sbjct: 481 VAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAVSFLPKPDV 553
            NF YFRFFA  FLPKP+V
Sbjct: 541 FNFLYFRFFATHFLPKPNV 556

BLAST of Chy8G153130 vs. NCBI nr
Match: KAG6603629.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 472/560 (84.29%), Postives = 503/560 (89.82%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           ME+G L HSS++  R K   GGWRAVRFILGNETFEKLASMSLI NLVLYLHTMYNLD+V
Sbjct: 1   MESGRL-HSSVN--RPKIALGGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIW GTTNFA LAGAFLADAYLGRFYTLLFGS ASLLGMGVLTLGAG P+MRPP
Sbjct: 61  DSAYVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC  GESNCPQP PWQLGFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWY LFS+AL+IALT+VVYVQT+ISWTLGF IPTACFVFSI IFLLG R+YICK PQG
Sbjct: 181 LNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQG 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQ-LHNPPMNSSKLAHTNRFLIFDKAATVVDSSEE 300
           SVFADMAKV+VATCRK  +PK TQ D  LH+PPM+SSKL+HT+RFL+FDKAATVVDSS E
Sbjct: 241 SVFADMAKVLVATCRKRNVPKFTQIDHFLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTE 300

Query: 301 LDEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSI 360
           LDE+GKSKNEW+LCSVHQVEQFKC VGIVP+W AGIPCF+SMQQM SFGILQAIQMNR I
Sbjct: 301 LDEQGKSKNEWKLCSVHQVEQFKCFVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFI 360

Query: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCM 420
           GPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT  NKRFS+KTRIEIGIVMSVLCM
Sbjct: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSLKTRIEIGIVMSVLCM 420

Query: 421 VVAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLR 480
           VVAG+LE  RR +A ENKSF SPLHVW+LIPEFALSGLTEAF AIA+MELLTTHLPESLR
Sbjct: 421 VVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLR 480

Query: 481 TVAGAIFFLSLSLASYLSSVLTGIVREVERE------WLGGNDLNKNRLDYFFYVVGVIA 540
           TVAGAIFFLSLSL SY+SSVL  IV ++ R       WLGGNDLNKNRLDYFFYVVGVIA
Sbjct: 481 TVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIA 540

Query: 541 ALNFFYFRFFAVSFLPKPDV 553
           A NFFYFRFFA  FLPKP+V
Sbjct: 541 AFNFFYFRFFATHFLPKPNV 557

BLAST of Chy8G153130 vs. NCBI nr
Match: XP_022931895.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita moschata])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 456/576 (79.17%), Postives = 503/576 (87.33%), Query Frame = 0

Query: 1   MENGNLLHSSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           M+NG L  SSLD S K +  GGWRAVRFIL NETFEKLASMSLI NL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSPKPQL-GGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIAS LGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC SG+++C Q   WQL FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWYFLFSIAL+IALT+VVY+QTNISWTLGF IPTA F+FSI IFLLG   YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVVVATCRKCQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSEEL 300
           SVFADMAKV++AT RK QIP+ +  + LH+PPM+SSKL HTNRFL+FDKAA V+DSS EL
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSP-NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DE+G+SKNEW+LC+VHQVEQ KCVVGIVP+W AGI CF+SMQQM SFGILQAIQMNR +G
Sbjct: 301 DEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHF+IPPAWMSL PMIALS+WIYIYEKYVE M++KT  NKRFSMK RIEIGIVMSVLCM+
Sbjct: 361 PHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCMI 420

Query: 421 VAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRT 480
           VAG+LEK RRD+A+EN++F SPLHV VLIPEFALSGLTEAF AI++MELLTT LPESLRT
Sbjct: 421 VAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLTGIVREVER------EWLGGNDLNKNRLDYFFYVVGVIAA 540
           VAGAIFFLSLSLASYLSSVL GIV  V +       WL GNDLNKN LDYFFYVVGVIAA
Sbjct: 481 VAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAELG 570
           LNFFYFRF    FLP+ DVDPR +A +R+ ED E G
Sbjct: 541 LNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESG 573

BLAST of Chy8G153130 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.5e-160
Identity = 285/537 (53.07%), Postives = 379/537 (70.58%), Query Frame = 0

Query: 17  KETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFA 76
           K+ +GGWRA+++I+ NE+FEKLASMSLIGNL +YL T YNL  V    V  IW G+ N  
Sbjct: 15  KKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNIL 74

Query: 77  PLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGESNCPQPLPWQ 136
            LAGAF++DAYLGRF+TLL GSIAS +GMG+  L A LP +RP  CI   +   QP  WQ
Sbjct: 75  TLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQ 134

Query: 137 LGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIAL 196
           LG L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWYF F++AL+IAL
Sbjct: 135 LGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIAL 194

Query: 197 TIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRK 256
           T VVY+QTNISW +GF IPTAC   SI  F++G   YIC   +GSVFAD+ KVV A C+K
Sbjct: 195 TGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKK 254

Query: 257 CQIPKTTQFDQLHNPPMNSSK---LAHTNRFLIFDKAATVVDSSEELDEEGKSKNEWRLC 316
            ++   +       P  + S    +   +R   FDK A++V +  EL+E+G +K +WRLC
Sbjct: 255 RKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDK-ASIVTNPNELNEDGNAKYKWRLC 314

Query: 317 SVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPH-FQIPPAWMSL 376
           SV QV+  KCV  I+PVW+ GI CF+   Q + +GILQA+QM+++ GPH FQ+P  WM+L
Sbjct: 315 SVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNL 374

Query: 377 TPMIALSIWIYIYE-KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFRRD 436
             MI L+IWI +YE   +  +K+ T   KR ++K RIE  IVM ++CM+VAG  EK RR 
Sbjct: 375 VSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRA 434

Query: 437 AAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRTVAGAIFFLSLS 496
           +A++N SF SP+ + +L+P+FAL+GLTEAF A+A+ME LT  +PE +R VAGAIFFLS S
Sbjct: 435 SALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSS 494

Query: 497 LASYLSSVLTGIVREVERE----WLGGNDLNKNRLDYFFYVVGVIAALNFFYFRFFA 545
           +ASY+ ++L  ++  V R+    WLG  DLNKNRL+ +F+++  I   N  YFR FA
Sbjct: 495 IASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLFA 548

BLAST of Chy8G153130 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 417.2 bits (1071), Expect = 2.2e-116
Identity = 221/576 (38.37%), Postives = 350/576 (60.76%), Query Frame = 0

Query: 9   SSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 68
           S LDA + ++  GGWRAV FILGNET E+L S+ L+ N ++YL  +++L+ V +A V  I
Sbjct: 41  SILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINI 100

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGES- 128
           W G TN  PL GA+++D Y+GRF T+ F S A+LLG+  +TL A  PQ+ P  C S +  
Sbjct: 101 WSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPL 160

Query: 129 NCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFL 188
           +C  P   Q+G L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  
Sbjct: 161 SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMT 220

Query: 189 FSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMA 248
           F++ LII  T+VVY+Q  +SW +GF IPT     ++ +F  G + Y+   P+GS+F+ +A
Sbjct: 221 FTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIA 280

Query: 249 KVVVATCRKCQIPKTTQFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSEELD 308
           +V+VA  +K ++    + D     ++P + S   SKL  +N+F   DKAA V++   +L 
Sbjct: 281 QVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLT 340

Query: 309 EEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGP 368
            EG   ++WRLCSV +VE+ KC++ IVP+W AGI    +M    +F + QA++M+R++GP
Sbjct: 341 PEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGP 400

Query: 369 HFQIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 428
            F+IP   +S+  ++ + I++  Y++ +V  M++ T      ++  RI  GIV ++  M+
Sbjct: 401 KFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMI 460

Query: 429 VAGVLEKFRRDAAVE--NKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESL 488
           VAG++E+ RR  ++   + +  +P+ V+ L P+  L GL EAF  I  +E   +  PE +
Sbjct: 461 VAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHM 520

Query: 489 RTVAGAIFFLSLSLASYLSSVLTGIVREV-----EREWLGGNDLNKNRLDYFFYVVGVIA 548
           R++A ++F LS + +SYLSS L  +V +        +WL  N LN  +LDYF+Y++ V+ 
Sbjct: 521 RSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLG 580

Query: 549 ALNFFYFRFFAVSFLPKPDVDPRQRAHQRQLEDAEL 570
            +N  YF + A  +  K  +        +  +D E+
Sbjct: 581 VVNLVYFWYCARGYRYKVGLPIEDFEEDKSSDDVEM 613

BLAST of Chy8G153130 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 415.2 bits (1066), Expect = 8.4e-116
Identity = 228/568 (40.14%), Postives = 347/568 (61.09%), Query Frame = 0

Query: 21  GGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFAPLAG 80
           GGWRA+ FILGNET EKL S+ +  N +LYL  +++++ V +  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 81  AFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGE-SNCPQPLPWQLGF 140
           A ++DAY+GRF T+ + S+ S+LG+  +TL A LPQ+ PPPC +     C  P   QLG 
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 141 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTIV 200
           L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ LI + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 201 VYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRKCQI 260
           VY+QT +SW +GF IPT+    ++ +F +G R+Y+   P+GSVF+ +A+V+VA  +K  +
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 261 PKTTQFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSEELDEEGKSKNEWRLC 320
             +   D   + + PP+     SKL  T++F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 321 SVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHFQIPPAWMSLT 380
           S+ +VE+ KC++ +VPVW AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 381 PMIALSIWIYIYE----KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFR 440
             I + IW+ IYE     ++  M+K      R ++  R+ IGIV ++L M  AG +E  R
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRMRK-----FRVTLLQRMGIGIVFAILSMFTAGFVEGVR 435

Query: 441 RDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLRTVAGAIFFLS 500
           R  A E    T     W+ +P   L GL E+F  I ++E   +  PE +R++A ++F LS
Sbjct: 436 RTRATE---MTQMSVFWLALP-LILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLS 495

Query: 501 LSLASYLSSVLTGIVREV-----EREWLGGNDLNKNRLDYFFYVVGVIAALNFFYFRFFA 560
            + A+YLSS+L   V +V       +WL   DL++ +LDYF+Y++ V+  +N  YF + A
Sbjct: 496 FAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCA 555

Query: 561 VSFLPKPDVDPRQRAHQRQLEDAELGRR 573
             +  K          ++ L D E  +R
Sbjct: 556 HRYQYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of Chy8G153130 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 4.0e-102
Identity = 197/545 (36.15%), Postives = 331/545 (60.73%), Query Frame = 0

Query: 9   SSLDASRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 68
           SS D + ++    GW+A+ +I+GNET E+LA+  L+ N ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPCISGESN 128
           W   TNFAP+ GAF++D+Y G+F T++FGSIA LLGM VLT  + +P +RPPPC + +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPC-TADQI 127

Query: 129 CPQPLPWQLGFLYL---GLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY 188
             Q +P+    LY+   GL L+ +G GGIR C++ F  DQFD +TE+GR    SF +W+Y
Sbjct: 128 TGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYY 187

Query: 189 FLFSIALIIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFAD 248
              +I  ++++T+V+YVQ NISW +GF IPT    F++ +  +G RYY+   P+GSVF+ 
Sbjct: 188 TTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSG 247

Query: 249 MAKVVVATCRKCQIPKTTQFDQLHNP----PMNSSKLAHTNRFLIFDKAATVVDSSEELD 308
           + KV+VA  +K +   T+  D  H P     + S+KL  T++F   +KA  V+++ E  +
Sbjct: 248 VFKVLVAAYKKRKARFTSGID-YHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNNDEAGN 307

Query: 309 EEGKSKNEWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSI-G 368
           E      EWR C+V Q+E  K ++ I+P++ + I  F++M Q  +F + QA++M+    G
Sbjct: 308 E------EWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPG 367

Query: 369 PHFQIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCM 428
             + IPPA +++  ++ + IW+  YE   V H++  T+ N   S+  ++ IG + S+  M
Sbjct: 368 TSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTM 427

Query: 429 VVAGVLEKFRRDAAVENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESLR 488
           +++G++E+ RRD ++        + V+ L P+  L G  + F  + + E     +P ++R
Sbjct: 428 LISGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMR 487

Query: 489 TVAGAIFFLSLSLASYLSSVLTGIVREVE----REWLGGNDLNKNRLDYFFYVVGVIAAL 541
           ++  ++ +L LSLASYLSS +  IV  V     + WL  +D++K++LD F+Y +  ++ L
Sbjct: 488 SIGNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTL 539

BLAST of Chy8G153130 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 4.0e-102
Identity = 204/546 (37.36%), Postives = 321/546 (58.79%), Query Frame = 0

Query: 14  SRKKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 73
           ++KK    GW+ + FI+GNETFEKL  +  + NL++YL  ++NL ++ +A +   + GT 
Sbjct: 38  NQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTI 97

Query: 74  NFAPLAGAFLADAYLGRFYTLLFGSIASLLGMGVLTLGAGLPQMRPPPC-ISGESNCPQP 133
           NF     AFL D Y GR+ TL    IA  LG  V+ L A +PQ+ P  C  + +S C  P
Sbjct: 98  NFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGP 157

Query: 134 LPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIAL 193
              Q+ FL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A 
Sbjct: 158 SGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQ 217

Query: 194 IIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVA 253
           I++LT+VVYVQ+N+SWT+G  IP      +  IF  G + Y+     GS  A +A+V+  
Sbjct: 218 ILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAV 277

Query: 254 TCRKCQI-PKTTQFDQLHN---PPMNSSKLAHTNRFLIFDKAATVVDSSEELDEEGKSKN 313
             +K  + P    +  L+N   P   +SKL +T++F   DKAA ++   ++L  +GK  +
Sbjct: 278 AIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPAD 337

Query: 314 EWRLCSVHQVEQFKCVVGIVPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGP-HFQIPP 373
            W+LC++ QVE+ KC+V ++P+W A    ++++ Q  ++ + QA+Q +R +G   F IP 
Sbjct: 338 PWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPA 397

Query: 374 AWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLE 433
           A   +  M  ++++I +Y++  V  M++ T  +   ++  RI  GI  +   +VVAG +E
Sbjct: 398 ATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVE 457

Query: 434 KFRRDAAV--------ENKSFTSPLHVWVLIPEFALSGLTEAFGAIAIMELLTTHLPESL 493
           + RR  A+          K   S +    LIP+ +L+G+ EAF AI  ME      PE++
Sbjct: 458 ERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENM 517

Query: 494 RTVAGAIFFLSLSLASYLSSVLTGIVREVEREWLGGN----DLNKNRLDYFFYVVGVIAA 541
           R+ AG+IF++   ++SYL S L   V    +   GGN    DLNK RLD F++++  I A
Sbjct: 518 RSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILA 577