Chy8G151680 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G151680
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	tRNA_int_end_N2 domain-containing protein
Location	chrH08: 13224917 .. 13226891 (-)
RNA-Seq Expression	Chy8G151680
Synteny	Chy8G151680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCTACGAATTGGGAAAGCTCTTCAGGAGGAGCTAGTGATACTGGTGATGAAGACAATTACGAACAAGACATAAACGACGAAGAAGAATGTCTCAGTGCATCTGGGTGCCTGCGCAAGTTGCAATTCAGGTTGAGGATTTCAAGAAGTTTAACTCCTCTGAATCCCTCAATAGGATGATGCTATTTGATTATTATCAAGTGTGTTACAATATTTTATCTATTTCTAGGAAGCATGCTTCAACTGCTCGATGGAATGACCAAATGGGAATGGCAGAAGTTTTAGAGAATAAGGGCAGCCTTTGGACGACAACTGGCATTGTACGTTGTGGCAAGATTTATTGTTCCATTGAGGAAACTTTGTAGGTTAAACCATGAGTTTATTTTGGTTTTGTTATCCTGATTGTTTTTTGATATTTACCACAACATTTTTTGGGGAGTTCTTCTCTTTCGCATTGTCTTAATTTGATTTCTTTTAAGTAATAGGGTAATGTCATTGAAAACAAAAGAAATTGGAAACGTTTCCAAGCCAAGGCCAGAAATTGTAGCCTATGAGGTGAGGAGAGCTGCATTAGCTGCATCTTGCAATTACCATGGTCGCCTCTTGGTCAGTTTTTTTAATTGCGTTTTGGTTGTCTTTGGGCCTGTATTTAGACCATTACATTATGAATAAAGAGAAGAAATTCTAACCAAAAATATACAAGAGGTAGCAGTTGTTGCCTTTTATGTCTTAGTAAAGACGAGAAATTACCATTTTGAAGTATTTTCTTTTGCTAAAATAAAGAAGTTTTTTCCTATCATTATCTCTTGAAAATAAGTAGCATCAATGTAATTTATTCAGCATGTTTGTGTCAAGTAATATATCGTTTTATGTATTCTTTTCTACTTACAATATATATTCATCTTCTAGTCTATTTACAATATCTATGTGCATTGTTTTACACTCACTTGGGCAACGTTTCTTAATAATTTTTTTTTTATTGTCTCTTAATAATTTCTTTTCTATATTTTCAGATTTCTTATTGAAGTCGGGGCCTTACATCTGCTGGATCATGATAATTCAAGTCTTTCTTTGAAGGATGTATATAAGAAGGTAGCTGAAGGAAGAAGTGGCTGTCCTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTACATTGTTGGAAAGCACAACGTTCCTTGGTCTCTAAAGAATGTTAGGAATGACTGTGACATTTCATCTCCAAGTTCTACTGAAAATAAAGGAGCATCAGATGTCAAATCAGAAGATGAGCAGTCGATTTCCAGGCTATTAAATGCTATTCAGCTCGATGAAGTGACACCCATTTTTGATGTTTTTCTTCCACATAACAAGTTTAGAAAATCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAGGTAAACATTTTCCTCTATAAGATAGTCTGCAGTATGAGTTTTGTGTTCAATTTCTTTGTCAACTACACTTAGTTAAATGCTTAAGTGACACACATATGATGTAGGCAGTTTTGGAAGAAAATTAGTTAAATGTTTCCTGTATAAACACTCGATATATTTTTTACTAGTTTCTAGAATTTCTCAAACTACTTCTCATTATTAAATCTTTTTAAAAATGCATTTAGTCAATGGTTCTGAAATTGTTTAGGAATTGTATCGATTTTCTTTTTTAGCCTTTTGCGACTATTTAGTTGCATTCATGTAATAACACTAATATCTAATAATAGGTTTCGACTCGTGTTGTGCCTTTCATCTGGGAAGTTCTATGCATTACTTAGAATTGATTTGATATTCATGTGATATAATTTTATTTCTGTTATAGGGGATATCCACCATCAAAAGAAGAGATTGAAGTTCTTGAGAGAACATCAAGAGGCATTCCAATGAAATATTGTCACGTTGAACATGGAAGAGTCTGCTTCTTTTCATTTGATAAGGTGGAGCTCCCTCTCTTACCTTGA

mRNA sequence

ATGGAGGCTACGAATTGGGAAAGCTCTTCAGGAGGAGCTAGTGATACTGGTGATGAAGACAATTACGAACAAGACATAAACGACGAAGAAGAATGTCTCAGTGCATCTGGGTGCCTGCGCAAGTTGCAATTCAGGAAGCATGCTTCAACTGCTCGATGGAATGACCAAATGGGAATGGCAGAAGTTTTAGAGAATAAGGGCAGCCTTTGGACGACAACTGGCATTGTACGTTGTGGCAAGATTTATTGTTCCATTGAGGAAACTTTATTTCTTATTGAAGTCGGGGCCTTACATCTGCTGGATCATGATAATTCAAGTCTTTCTTTGAAGGATGTATATAAGAAGGTAGCTGAAGGAAGAAGTGGCTGTCCTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTACATTGTTGGAAAGCACAACGTTCCTTGGTCTCTAAAGAATGTTAGGAATGACTGTGACATTTCATCTCCAAGTTCTACTGAAAATAAAGGAGCATCAGATGTCAAATCAGAAGATGAGCAGTCGATTTCCAGGCTATTAAATGCTATTCAGCTCGATGAAGTGACACCCATTTTTGATGTTTTTCTTCCACATAACAAGTTTAGAAAATCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAGGGGATATCCACCATCAAAAGAAGAGATTGAAGTTCTTGAGAGAACATCAAGAGGCATTCCAATGAAATATTGTCACGTTGAACATGGAAGAGTCTGCTTCTTTTCATTTGATAAGGTGGAGCTCCCTCTCTTACCTTGA

Coding sequence (CDS)

ATGGAGGCTACGAATTGGGAAAGCTCTTCAGGAGGAGCTAGTGATACTGGTGATGAAGACAATTACGAACAAGACATAAACGACGAAGAAGAATGTCTCAGTGCATCTGGGTGCCTGCGCAAGTTGCAATTCAGGAAGCATGCTTCAACTGCTCGATGGAATGACCAAATGGGAATGGCAGAAGTTTTAGAGAATAAGGGCAGCCTTTGGACGACAACTGGCATTGTACGTTGTGGCAAGATTTATTGTTCCATTGAGGAAACTTTATTTCTTATTGAAGTCGGGGCCTTACATCTGCTGGATCATGATAATTCAAGTCTTTCTTTGAAGGATGTATATAAGAAGGTAGCTGAAGGAAGAAGTGGCTGTCCTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTACATTGTTGGAAAGCACAACGTTCCTTGGTCTCTAAAGAATGTTAGGAATGACTGTGACATTTCATCTCCAAGTTCTACTGAAAATAAAGGAGCATCAGATGTCAAATCAGAAGATGAGCAGTCGATTTCCAGGCTATTAAATGCTATTCAGCTCGATGAAGTGACACCCATTTTTGATGTTTTTCTTCCACATAACAAGTTTAGAAAATCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAGGGGATATCCACCATCAAAAGAAGAGATTGAAGTTCTTGAGAGAACATCAAGAGGCATTCCAATGAAATATTGTCACGTTGAACATGGAAGAGTCTGCTTCTTTTCATTTGATAAGGTGGAGCTCCCTCTCTTACCTTGA

Protein sequence

MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMAEVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGRSGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQSISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRGIPMKYCHVEHGRVCFFSFDKVELPLLP*

Homology

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BLAST of Chy8G151680 vs. ExPASy Swiss-Prot
Match: O74908 (Probable tRNA-splicing endonuclease subunit sen54 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen54 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 7.2e-06
Identity = 35/109 (32.11%), Postives = 55/109 (50.46%), Query Frame = 0


Query: 39  LRKLQFRKHASTARWNDQMGMAEVLENKGSLWTTTGIVRC-GKIYCSIEETLFLIEVGAL 98
           + +L   KHA  A WN Q GM+ V +  G L+ T G      +++   EETL+L+E G++
Sbjct: 74  VERLISAKHAIIATWNAQNGMSCVEKAHGPLFKTMGTADSQNRMWLLPEETLYLVERGSM 133

Query: 99  HLLDHDNSSLSLKDVYKKVAEGRSGCPWEQFEVYRHLKSLGYIVGKHNV 147
                +   +SL+ VY   A        E + VY HL+  G+ V + N+
Sbjct: 134 ECWSEEGLPMSLQAVYS--ASIPLCGSLENYLVYAHLRRCGFSVIRSNL 180

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BLAST of Chy8G151680 vs. ExPASy Swiss-Prot
Match: Q74ZJ5 (tRNA-splicing endonuclease subunit SEN54 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEN54 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.3e-04
Identity = 30/102 (29.41%), Postives = 51/102 (50.00%), Query Frame = 0


Query: 51  ARWNDQMGMAEVLENKGSLWTTTGIV-RCGKIYCSIEETLFLIEVGAL----------HL 110
           A W ++  MA +   +GS   T G V + G+ +  + E ++L+E G +            
Sbjct: 88  AYWEEEQRMARIPNARGSFTNTMGKVDKHGQCWLQLHEFVYLVERGTVSPYLALTVGDEK 147

Query: 111 LDHDNSSLSLKDVYKKVAEGRSGCPWEQFEVYRHLKSLGYIV 142
            +H++  LS++DVY   +        ++F VY HLK LG+IV
Sbjct: 148 SNHEDVLLSVQDVYALFSSTEE---LDEFLVYAHLKRLGFIV 186

[Close]

BLAST of Chy8G151680 vs. ExPASy TrEMBL
Match: A0A0A0KXV5 (tRNA_int_end_N2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G334710 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.4e-150
Identity = 262/267 (98.13%), Postives = 263/267 (98.50%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR
Sbjct: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           SG  WEQFEVYRHLKSLGYIVGKH VPWSLKNVRNDCDISSPSSTENKGASDVKS+DEQS
Sbjct: 121 SGRLWEQFEVYRHLKSLGYIVGKHRVPWSLKNVRNDCDISSPSSTENKGASDVKSDDEQS 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           I RLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG
Sbjct: 181 ICRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 268
           IPMKYCHVEHGRVCFFSFDKVELPLLP
Sbjct: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 267

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BLAST of Chy8G151680 vs. ExPASy TrEMBL
Match: A0A1S4E447 (uncharacterized protein LOC103501474 OS=Cucumis melo OX=3656 GN=LOC103501474 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 3.8e-143
Identity = 248/260 (95.38%), Postives = 252/260 (96.92%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEATNW+SSSGGASDTGDEDNYEQDINDEEECLSAS CLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWKSSSGGASDTGDEDNYEQDINDEEECLSASRCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTTTGIVRCGKIYC+IEETLFLIEVGALHLLDHDNS+LSLKDVYKKVAEGR
Sbjct: 61  EVLENKGSLWTTTGIVRCGKIYCTIEETLFLIEVGALHLLDHDNSTLSLKDVYKKVAEGR 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           SGC WEQFEVYRHLKSLGYIVGKH VPWSLKNVRNDCD+SSPSSTE KG SDVKSEDEQ 
Sbjct: 121 SGCIWEQFEVYRHLKSLGYIVGKHKVPWSLKNVRNDCDLSSPSSTEKKGVSDVKSEDEQL 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           IS LLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCL RGYPPSKEEIEVLERTSRG
Sbjct: 181 ISGLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLARGYPPSKEEIEVLERTSRG 240

Query: 241 IPMKYCHVEHGRVCFFSFDK 261
           IPMKYCHVEHGRVCFFSFDK
Sbjct: 241 IPMKYCHVEHGRVCFFSFDK 260

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BLAST of Chy8G151680 vs. ExPASy TrEMBL
Match: A0A6J1I8D9 (tRNA-splicing endonuclease subunit Sen54 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.2e-127
Identity = 222/267 (83.15%), Postives = 245/267 (91.76%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+WE SSGGASD  D+DN+EQDI +EEECL +SG +RKLQFRKHASTARWND+MGMA
Sbjct: 1   MEATDWEISSGGASD--DDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTT+GIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEG+
Sbjct: 61  EVLENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGK 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           S C WEQFEVYRHLKSLGYIVGKH VPWS+K  RN  DISS SS ENKG++D +SEDE+S
Sbjct: 121 SECIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSIENKGSTDFESEDEKS 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           I  LLNA+QL+E+TPIFDV+LPH+KFRKSSPGDPNFMVCLTRGYPP K +IEV+ER S G
Sbjct: 181 ICELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGG 240

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 268
           IPMKYCHVEHGRVCFFSFDKV+LP+LP
Sbjct: 241 IPMKYCHVEHGRVCFFSFDKVKLPVLP 265

[Close]

BLAST of Chy8G151680 vs. ExPASy TrEMBL
Match: A0A6J1ICR8 (tRNA-splicing endonuclease subunit Sen54 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.2e-127
Identity = 222/267 (83.15%), Postives = 245/267 (91.76%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+WE SSGGASD  D+DN+EQDI +EEECL +SG +RKLQFRKHASTARWND+MGMA
Sbjct: 5   MEATDWEISSGGASD--DDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMA 64

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTT+GIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEG+
Sbjct: 65  EVLENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGK 124

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           S C WEQFEVYRHLKSLGYIVGKH VPWS+K  RN  DISS SS ENKG++D +SEDE+S
Sbjct: 125 SECIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSIENKGSTDFESEDEKS 184

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           I  LLNA+QL+E+TPIFDV+LPH+KFRKSSPGDPNFMVCLTRGYPP K +IEV+ER S G
Sbjct: 185 ICELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGG 244

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 268
           IPMKYCHVEHGRVCFFSFDKV+LP+LP
Sbjct: 245 IPMKYCHVEHGRVCFFSFDKVKLPVLP 269

[Close]

BLAST of Chy8G151680 vs. ExPASy TrEMBL
Match: A0A6J1I794 (tRNA-splicing endonuclease subunit Sen54 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.2e-127
Identity = 222/267 (83.15%), Postives = 245/267 (91.76%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+WE SSGGASD  D+DN+EQDI +EEECL +SG +RKLQFRKHASTARWND+MGMA
Sbjct: 15  MEATDWEISSGGASD--DDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMA 74

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTT+GIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEG+
Sbjct: 75  EVLENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGK 134

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           S C WEQFEVYRHLKSLGYIVGKH VPWS+K  RN  DISS SS ENKG++D +SEDE+S
Sbjct: 135 SECIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSIENKGSTDFESEDEKS 194

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           I  LLNA+QL+E+TPIFDV+LPH+KFRKSSPGDPNFMVCLTRGYPP K +IEV+ER S G
Sbjct: 195 ICELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGG 254

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 268
           IPMKYCHVEHGRVCFFSFDKV+LP+LP
Sbjct: 255 IPMKYCHVEHGRVCFFSFDKVKLPVLP 279

[Close]

BLAST of Chy8G151680 vs. NCBI nr
Match: XP_011653726.1 (uncharacterized protein LOC101210680 isoform X2 [Cucumis sativus] >KGN54460.1 hypothetical protein Csa_012673 [Cucumis sativus])

HSP 1 Score: 534 bits (1375), Expect = 2.91e-191
Identity = 262/267 (98.13%), Postives = 263/267 (98.50%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR
Sbjct: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           SG  WEQFEVYRHLKSLGYIVGKH VPWSLKNVRNDCDISSPSSTENKGASDVKS+DEQS
Sbjct: 121 SGRLWEQFEVYRHLKSLGYIVGKHRVPWSLKNVRNDCDISSPSSTENKGASDVKSDDEQS 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           I RLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG
Sbjct: 181 ICRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 267
           IPMKYCHVEHGRVCFFSFDKVELPLLP
Sbjct: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 267

[Close]

BLAST of Chy8G151680 vs. NCBI nr
Match: XP_016902999.1 (PREDICTED: uncharacterized protein LOC103501474 [Cucumis melo])

HSP 1 Score: 509 bits (1310), Expect = 2.63e-181
Identity = 248/260 (95.38%), Postives = 252/260 (96.92%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEATNW+SSSGGASDTGDEDNYEQDINDEEECLSAS CLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWKSSSGGASDTGDEDNYEQDINDEEECLSASRCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTTTGIVRCGKIYC+IEETLFLIEVGALHLLDHDNS+LSLKDVYKKVAEGR
Sbjct: 61  EVLENKGSLWTTTGIVRCGKIYCTIEETLFLIEVGALHLLDHDNSTLSLKDVYKKVAEGR 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           SGC WEQFEVYRHLKSLGYIVGKH VPWSLKNVRNDCD+SSPSSTE KG SDVKSEDEQ 
Sbjct: 121 SGCIWEQFEVYRHLKSLGYIVGKHKVPWSLKNVRNDCDLSSPSSTEKKGVSDVKSEDEQL 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           IS LLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCL RGYPPSKEEIEVLERTSRG
Sbjct: 181 ISGLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLARGYPPSKEEIEVLERTSRG 240

Query: 241 IPMKYCHVEHGRVCFFSFDK 260
           IPMKYCHVEHGRVCFFSFDK
Sbjct: 241 IPMKYCHVEHGRVCFFSFDK 260

[Close]

BLAST of Chy8G151680 vs. NCBI nr
Match: XP_031739606.1 (uncharacterized protein LOC101210680 isoform X1 [Cucumis sativus])

HSP 1 Score: 491 bits (1263), Expect = 4.78e-174
Identity = 250/276 (90.58%), Postives = 253/276 (91.67%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA
Sbjct: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60

Query: 61  EVLENKGSLWTTTGI------VRCGKIYCSIEETL---FLIEVGALHLLDHDNSSLSLKD 120
           EVLENKGSLWTTTGI       R  K + +        FLIEVGALHLLDHDNSSLSLKD
Sbjct: 61  EVLENKGSLWTTTGIGNVIENKRNWKRFQAKARNWPMRFLIEVGALHLLDHDNSSLSLKD 120

Query: 121 VYKKVAEGRSGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGAS 180
           VYKKVAEGRSG  WEQFEVYRHLKSLGYIVGKH VPWSLKNVRNDCDISSPSSTENKGAS
Sbjct: 121 VYKKVAEGRSGRLWEQFEVYRHLKSLGYIVGKHRVPWSLKNVRNDCDISSPSSTENKGAS 180

Query: 181 DVKSEDEQSISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEI 240
           DVKS+DEQSI RLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEI
Sbjct: 181 DVKSDDEQSICRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEI 240

Query: 241 EVLERTSRGIPMKYCHVEHGRVCFFSFDKVELPLLP 267
           EVLERTSRGIPMKYCHVEHGRVCFFSFDKVELPLLP
Sbjct: 241 EVLERTSRGIPMKYCHVEHGRVCFFSFDKVELPLLP 276

[Close]

BLAST of Chy8G151680 vs. NCBI nr
Match: XP_038883355.1 (uncharacterized protein LOC120074337 [Benincasa hispida] >XP_038883356.1 uncharacterized protein LOC120074337 [Benincasa hispida] >XP_038883357.1 uncharacterized protein LOC120074337 [Benincasa hispida])

HSP 1 Score: 474 bits (1219), Expect = 1.62e-167
Identity = 232/267 (86.89%), Postives = 251/267 (94.01%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEA +WESSSGGAS  GDEDNYE+DIN+EEECL ASG LRKLQFRKHASTARWND+MGMA
Sbjct: 1   MEAVDWESSSGGAS--GDEDNYEEDINNEEECLCASGYLRKLQFRKHASTARWNDRMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSL+DVYKK+AEG+
Sbjct: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLEDVYKKIAEGK 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           +GC WEQFEVYRHLKSLG+IVGKH VPWSLK+VR+  +ISSPSS ENKGASDVKSEDE+S
Sbjct: 121 NGCLWEQFEVYRHLKSLGFIVGKHKVPWSLKSVRDGSNISSPSSIENKGASDVKSEDERS 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           IS LL+ IQL+EV PIFDVFLPHNKFRKSSPGDPNFMV LTRGYPPSK+++EVL RTSRG
Sbjct: 181 ISELLDGIQLEEVMPIFDVFLPHNKFRKSSPGDPNFMVYLTRGYPPSKKDLEVLARTSRG 240

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 267
           IPMKYCHVEHGRVCFFSFD VELP+LP
Sbjct: 241 IPMKYCHVEHGRVCFFSFDTVELPILP 265

[Close]

BLAST of Chy8G151680 vs. NCBI nr
Match: XP_022973340.1 (tRNA-splicing endonuclease subunit Sen54 isoform X3 [Cucurbita maxima] >XP_022973341.1 tRNA-splicing endonuclease subunit Sen54 isoform X3 [Cucurbita maxima])

HSP 1 Score: 457 bits (1175), Expect = 8.22e-161
Identity = 222/267 (83.15%), Postives = 245/267 (91.76%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           MEAT+WE SSGGASD  D+DN+EQDI +EEECL +SG +RKLQFRKHASTARWND+MGMA
Sbjct: 1   MEATDWEISSGGASD--DDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGR 120
           EVLENKGSLWTT+GIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEG+
Sbjct: 61  EVLENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGK 120

Query: 121 SGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQS 180
           S C WEQFEVYRHLKSLGYIVGKH VPWS+K  RN  DISS SS ENKG++D +SEDE+S
Sbjct: 121 SECIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSIENKGSTDFESEDEKS 180

Query: 181 ISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSRG 240
           I  LLNA+QL+E+TPIFDV+LPH+KFRKSSPGDPNFMVCLTRGYPP K +IEV+ER S G
Sbjct: 181 ICELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGG 240

Query: 241 IPMKYCHVEHGRVCFFSFDKVELPLLP 267
           IPMKYCHVEHGRVCFFSFDKV+LP+LP
Sbjct: 241 IPMKYCHVEHGRVCFFSFDKVKLPVLP 265

[Close]

BLAST of Chy8G151680 vs. TAIR 10
Match: AT3G57360.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G02370.2); Has 122 Blast hits to 122 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 74; Fungi - 4; Plants - 41; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 243.4 bits (620), Expect = 2.0e-64
Identity = 117/267 (43.82%), Postives = 175/267 (65.54%), Query Frame = 0


Query: 1   MEATNWESSSGGASDTGDEDNYEQDINDEEECLSASGCLRKLQFRKHASTARWNDQMGMA 60
           ME  +WE+SS   ++ G         ND++E   + G + KLQFR  +S ARW  ++GMA
Sbjct: 1   MEEKDWEASSSSENEGGFP-------NDDDEEFHSGGSVPKLQFRVGSSKARWITELGMA 60

Query: 61  EVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLL-DHDNSSLSLKDVYKKVAEG 120
           EV   +G LWTTTGI+R GK YC IEE L+L E+G L +L + D+  + LKD+Y+K+AE 
Sbjct: 61  EVEVKRGKLWTTTGIIRSGKTYCFIEEALYLSEIGELQILGNEDDIVIPLKDLYEKIAEE 120

Query: 121 RSGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSEDEQ 180
           +SGC WE +EVYR+LK LGYI+G+H V W+LK      D + P+  E    +     D  
Sbjct: 121 KSGCSWENYEVYRYLKGLGYILGRHGVSWTLK------DAARPNGEEESACAGECPADND 180

Query: 181 SISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIEVLERTSR 240
           ++++LL  +Q+ +   +FDV+LP+++F+KSSPG+P+F+ C +   PPSKE+I+VL++   
Sbjct: 181 TVTKLLGDMQICDAKAVFDVYLPNSRFKKSSPGEPSFVACFSGDSPPSKEDIKVLQKRI- 240

Query: 241 GIPMKYCHVEHGRVCFFSFDKVELPLL 267
             P+ +CH+  GR  FFSF  ++LP+L
Sbjct: 241 AAPLTFCHIAQGRASFFSFSSIDLPVL 253

[Close]

BLAST of Chy8G151680 vs. TAIR 10
Match: AT3G02370.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57360.1). )

HSP 1 Score: 184.5 bits (467), Expect = 1.1e-46
Identity = 86/175 (49.14%), Postives = 124/175 (70.86%), Query Frame = 0


Query: 59  MAEVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLL-DHDNSSLSLKDVYKKVA 118
           MAEV   +G LWTTTGI+R GK YC IEE L+L E+G L LL D D+  +SLKD+Y ++A
Sbjct: 1   MAEVEVKRGKLWTTTGIIRTGKTYCFIEEALYLSEIGELQLLGDEDDVVISLKDLYGEIA 60

Query: 119 EGRSGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSED 178
           EG+ GC WE +EVYR+LK LGYI+G+H VPW+ K   N    ++PS  +    +    +D
Sbjct: 61  EGKYGCCWENYEVYRYLKGLGYILGRHGVPWTTKYAVN----TTPSDEDESLCAAEFFQD 120

Query: 179 EQSISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLTRGYPPSKEEIE 233
             S+++LL+ + + +  P+FDV+LP+++F+KSSPG+P+F+ C +   PPSKEEI+
Sbjct: 121 RDSVTKLLSDMHICDARPVFDVYLPNSQFKKSSPGEPSFVTCFSGDSPPSKEEIK 171

[Close]

BLAST of Chy8G151680 vs. TAIR 10
Match: AT3G02370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57360.1); Has 95 Blast hits to 95 proteins in 40 species: Archae - 0; Bacteria - 0; Metazoa - 53; Fungi - 2; Plants - 39; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 171.4 bits (433), Expect = 9.8e-43
Identity = 79/164 (48.17%), Postives = 116/164 (70.73%), Query Frame = 0


Query: 59  MAEVLENKGSLWTTTGIVRCGKIYCSIEETLFLIEVGALHLL-DHDNSSLSLKDVYKKVA 118
           MAEV   +G LWTTTGI+R GK YC IEE L+L E+G L LL D D+  +SLKD+Y ++A
Sbjct: 1   MAEVEVKRGKLWTTTGIIRTGKTYCFIEEALYLSEIGELQLLGDEDDVVISLKDLYGEIA 60

Query: 119 EGRSGCPWEQFEVYRHLKSLGYIVGKHNVPWSLKNVRNDCDISSPSSTENKGASDVKSED 178
           EG+ GC WE +EVYR+LK LGYI+G+H VPW+ K   N    ++PS  +    +    +D
Sbjct: 61  EGKYGCCWENYEVYRYLKGLGYILGRHGVPWTTKYAVN----TTPSDEDESLCAAEFFQD 120

Query: 179 EQSISRLLNAIQLDEVTPIFDVFLPHNKFRKSSPGDPNFMVCLT 222
             S+++LL+ + + +  P+FDV+LP+++F+KSSPG+P+F+ C +
Sbjct: 121 RDSVTKLLSDMHICDARPVFDVYLPNSQFKKSSPGEPSFVTCFS 160

The following BLAST results are available for this feature:

BLAST of Chy8G151680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O74908	7.2e-06	32.11	Probable tRNA-splicing endonuclease subunit sen54 OS=Schizosaccharomyces pombe (...	[more]
Q74ZJ5	2.3e-04	29.41	tRNA-splicing endonuclease subunit SEN54 OS=Ashbya gossypii (strain ATCC 10895 /...	[more]

BLAST of Chy8G151680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXV5	1.4e-150	98.13	tRNA_int_end_N2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G33...	[more]
A0A1S4E447	3.8e-143	95.38	uncharacterized protein LOC103501474 OS=Cucumis melo OX=3656 GN=LOC103501474 PE=...	[more]
A0A6J1I8D9	2.2e-127	83.15	tRNA-splicing endonuclease subunit Sen54 isoform X3 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1ICR8	2.2e-127	83.15	tRNA-splicing endonuclease subunit Sen54 isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1I794	2.2e-127	83.15	tRNA-splicing endonuclease subunit Sen54 isoform X1 OS=Cucurbita maxima OX=3661 ...	[more]

BLAST of Chy8G151680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653726.1	2.91e-191	98.13	uncharacterized protein LOC101210680 isoform X2 [Cucumis sativus] >KGN54460.1 hy...	[more]
XP_016902999.1	2.63e-181	95.38	PREDICTED: uncharacterized protein LOC103501474 [Cucumis melo]	[more]
XP_031739606.1	4.78e-174	90.58	uncharacterized protein LOC101210680 isoform X1 [Cucumis sativus]	[more]
XP_038883355.1	1.62e-167	86.89	uncharacterized protein LOC120074337 [Benincasa hispida] >XP_038883356.1 unchara...	[more]
XP_022973340.1	8.22e-161	83.15	tRNA-splicing endonuclease subunit Sen54 isoform X3 [Cucurbita maxima] >XP_02297...	[more]

BLAST of Chy8G151680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57360.1	2.0e-64	43.82	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G02370.2	1.1e-46	49.14	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G02370.1	9.8e-43	48.17	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024336	tRNA-splicing endonuclease, subunit Sen54, N-terminal	PFAM	PF12928	tRNA_int_end_N2	coord: 43..99 e-value: 1.6E-9 score: 37.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
IPR024337	tRNA-splicing endonuclease, subunit Sen54	PANTHER	PTHR21027	TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54	coord: 1..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G151680.1	Chy8G151680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0000379	tRNA-type intron splice site recognition and cleavage
cellular_component	GO:0000214	tRNA-intron endonuclease complex
molecular_function	GO:0004519	endonuclease activity