Chy7G144200 (gene) Cucumber (hystrix) v1

Overview


Name	Chy7G144200
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Ribonuclease 3-like protein 1
Location	chrH07: 20056713 .. 20059019 (+)
RNA-Seq Expression	Chy7G144200
Synteny	Chy7G144200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACACTACACAGACATCGTCGGAGCAGACCGGAAGAATCGAGGAATTCAGATGGAGAACAATCCTTCTTGTCCTCCTCCAAGATTGAATATCAATCTCAAAAATCTTCCTCCAATTAATAACCCGCGCAATATATCTGGTAGTCACTCCTCGATTCCGGTACAGCTCTGTTGATTTCCTGCGATTGTATTGGATTTTCTTGGGAATTTGAAACCCTTTTGATTTCGATCTGACTTGTTTCAGGCTAAATTTGAGAAATCTAGGTATATTAGGCGCGTTACTCAGTTTATGCCGTCTGATCACCGTCCGGAAATAAGGTCGAATGATTACGCTGGCTTGCCGATGAAACTATCGTATTCTGATTCCGCTCGAGCTCTGAATACGAAGACGATTCCGGTGGCTCCGTCGGCGGATTCCTTCGTTCAAAAGAAAGATAATTGCAAGGATTTAGCTTCGAGGTGTAGTTGTTGCAGACATGGCTCGACCAAAGAAGGTGCCTGTTTAAACCTAGTTCCTCAACTTCATTGATTGTTGTTTGGAATTCGAGATAGATGATTCTTGTTATGTGTTATCATTTTCGGTGATTGTTGTTTTCTTCGTGATTTTATGATTTTCTTCTAGCAATTGATTGTTCAACTGAACTCACATGCATTTCATCATGAACACCTTCTTTATCGCCTGTTTACCCCTAATCACTTCCCAATTCGTCATTAATATTTGTCCATTAATTTCAAAGTGCAGTCTAAAAAAAAAGAAGAAAGAAAATGCAAGCATTTGGTCTCTAAAAATTTTCCAAAACAGAACTCTGGATCAAAACCATCTTCTGGATCATGTTTGAACAGCTTAGTTTCTTCCAATTTTCTCTCTCTTGTTTTTCTTTCGATTTTTCTCAGGATGACTTGTTTTCAATAATGAATGAAGTTGTGTGTGAATGATAACATGCATTAGACTTCATAAATCATTCGATCACAATCCCTAATGGTTTCTTGAGAGTTGTTTCTGAATTTATGTTTGTTTCCTCTCAATTTTTCAGTTATAATTTTTAGTTAGTCAGAGAAACACTCATCAAATTCTAAAATTAATATGTTTTTGAAAACTATTTATTTAGTTTTCAAAACTCTGACTTGTTTTATTTGAAATATCAATAGAAAGTGAATTACAAAAATAGAAACTTATGGGTGTTATTAGTATTTATAAACTTATGGTTATATAATGACTTATAATTCAGTACTTTCTCTTTCTCCAAAGAACAAGTTATGATGCTCTGCTCTTGAGAGTTGTTCTTTGGAGTTGAGCTCTCTAAAGACTAGTTACTCAAACATGTTTATACCTTTTTTCCCCTTAATTAAAAAGATGGAATATAATTTTGCAAGCACATAAGCTTTTTCACTTTGATGGCTTACTTTATCTTTGTTTTAGTTGGATGAGGAAGACTACTTATTGCCTATCTGATTTCCATAAGCGTTGCCATGGCGCTTCAGAAATTTTGATTTTGACTACTCAAAGTTTGCTCTAATGGTTTGGCTTCACTCTTTTTTATTTTAGGTACTCCAGAAAAGAGAGCGGCAAAGTCCCTTCTATTTGAGATATGCACTGCAAATCACTGGCAGCCTCCTCTATTTGAATGCTGTGAAGAAGAAGGTCCAAGCCATGCAAAAAAGTAAGCTCAATATATAACTCTCATCAGCATAAGTTATAAACTTACAAACTCTTTGCTCAAGTAACCTGTCAGAGCCAAACTTTCAACGATACTTGGCTAAAGATGTATTCCTTATTATGCCTTTTGGTCTTGTAGATTATGCTCTCTTCTAACAACTACTTTTTGAAGCTTTTATAACGTTTACAGCCTTACTTGAACAAAATTTGTTTTATAAGTTTGTGTACAACTCTGTTTCTTCTTTTTATATTCGAAACGGGTACGTACAATTAGATGCCACCATGGAGTCAGCTGATGAAGGTTTGTTGTTAGCTATGTTTGGATTTGACATGTCCATATAGCATCTGTACTGTGCAAACATGAGCAAGAATATATAGATCGAATCAAACACCCGAGCAAAGAATGAAATTAGTAACTAGCTTCATTTATGGAAAACAGGGTTATTTGATGGGATTATAACAATTGATGCAAAATTTTGATTTTGATTCAGGTATAGATTCAAGGTTAGAATTGAAATGAAGGGTGATTGTGAAGCAGTTGTGGAATGCTATGGAAACCTTCAAACAAGAAAGAAAGTGGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAACCATTTGGGATATCGTTTTAAAAGATAA

mRNA sequence

ATGAGACACTACACAGACATCGTCGGAGCAGACCGGAAGAATCGAGGAATTCAGATGGAGAACAATCCTTCTTGTCCTCCTCCAAGATTGAATATCAATCTCAAAAATCTTCCTCCAATTAATAACCCGCGCAATATATCTGGTAGTCACTCCTCGATTCCGGCTAAATTTGAGAAATCTAGGTATATTAGGCGCGTTACTCAGTTTATGCCGTCTGATCACCGTCCGGAAATAAGGTCGAATGATTACGCTGGCTTGCCGATGAAACTATCGTATTCTGATTCCGCTCGAGCTCTGAATACGAAGACGATTCCGGTGGCTCCGTCGGCGGATTCCTTCGTTCAAAAGAAAGATAATTGCAAGGATTTAGCTTCGAGGTGTAGTTGTTGCAGACATGGCTCGACCAAAGAAGGTACTCCAGAAAAGAGAGCGGCAAAGTCCCTTCTATTTGAGATATGCACTGCAAATCACTGGCAGCCTCCTCTATTTGAATGCTGTGAAGAAGAAGGTCCAAGCCATGCAAAAAAGTATAGATTCAAGGTTAGAATTGAAATGAAGGGTGATTGTGAAGCAGTTGTGGAATGCTATGGAAACCTTCAAACAAGAAAGAAAGTGGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAACCATTTGGGATATCGTTTTAAAAGATAA

Coding sequence (CDS)

ATGAGACACTACACAGACATCGTCGGAGCAGACCGGAAGAATCGAGGAATTCAGATGGAGAACAATCCTTCTTGTCCTCCTCCAAGATTGAATATCAATCTCAAAAATCTTCCTCCAATTAATAACCCGCGCAATATATCTGGTAGTCACTCCTCGATTCCGGCTAAATTTGAGAAATCTAGGTATATTAGGCGCGTTACTCAGTTTATGCCGTCTGATCACCGTCCGGAAATAAGGTCGAATGATTACGCTGGCTTGCCGATGAAACTATCGTATTCTGATTCCGCTCGAGCTCTGAATACGAAGACGATTCCGGTGGCTCCGTCGGCGGATTCCTTCGTTCAAAAGAAAGATAATTGCAAGGATTTAGCTTCGAGGTGTAGTTGTTGCAGACATGGCTCGACCAAAGAAGGTACTCCAGAAAAGAGAGCGGCAAAGTCCCTTCTATTTGAGATATGCACTGCAAATCACTGGCAGCCTCCTCTATTTGAATGCTGTGAAGAAGAAGGTCCAAGCCATGCAAAAAAGTATAGATTCAAGGTTAGAATTGAAATGAAGGGTGATTGTGAAGCAGTTGTGGAATGCTATGGAAACCTTCAAACAAGAAAGAAAGTGGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAACCATTTGGGATATCGTTTTAAAAGATAA

Protein sequence

MRHYTDIVGADRKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEKSRYIRRVTQFMPSDHRPEIRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR*

Homology

[Close]

BLAST of Chy7G144200 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 3.6e-22
Identity = 46/88 (52.27%), Postives = 58/88 (65.91%), Query Frame = 0


Query: 136  KEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVEC 195
            K G P  + AKSLL E C AN W+PP FECCEEEGP H K + +KV +E++      +EC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 196  YGNLQTRKKVAAEHAAEGALWYLNHLGY 224
            YG  +  KK AAEHAA+ A+W L H G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Chy7G144200 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.7e-21
Identity = 41/81 (50.62%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 143  RAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYGNLQTR 202
            + A+S LFE+C AN+W+PP F+ C+EEGPSH +K+ +KV +E+KG    ++EC+ + + +
Sbjct: 1568 KTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGASATLLECHSDGKLQ 1627

Query: 203  KKVAAEHAAEGALWYLNHLGY 224
            KK A EHAA+GALW L  LG+
Sbjct: 1628 KKAAQEHAAQGALWCLKQLGH 1648

[Close]

BLAST of Chy7G144200 vs. ExPASy Swiss-Prot
Match: Q9M8N2 (Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 9.7e-20
Identity = 39/86 (45.35%), Postives = 61/86 (70.93%), Query Frame = 0


Query: 137 EGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDC-EAVVEC 196
           E    + +AKS+L E+C +  W+PP++ECC  +GP H + + +KV +E++    + V+EC
Sbjct: 107 ESKTRRGSAKSVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLEC 166

Query: 197 YGNLQTRKKVAAEHAAEGALWYLNHL 222
           +G+ + +KK AAEHAAEGALWYL H+
Sbjct: 167 FGDPRRKKKAAAEHAAEGALWYLEHV 192

[Close]

BLAST of Chy7G144200 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 3.0e-05
Identity = 39/142 (27.46%), Postives = 61/142 (42.96%), Query Frame = 0


Query: 78   IRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNCKDLASRCSCCRHGSTKE 137
            +  N    +  KL+  ++  AL  K I  A S +  +   +  +D         +G+ K 
Sbjct: 1773 VAQNPQKKMAQKLAARNALAALKEKEI--AESKEKHINNGNAGEDQGEN----ENGNKKN 1832

Query: 138  GTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYG 197
            G   +   +  L +IC   +W  P + C +E GP+HAK++ F VR+    D     EC G
Sbjct: 1833 G--HQPFTRQTLNDICLRKNWPMPSYRCVKEGGPAHAKRFTFGVRVN-TSDRGWTDECIG 1892

Query: 198  NLQTRKKVAAEHAAEGALWYLN 220
                  K A + AA   L  LN
Sbjct: 1893 EPMPSVKKAKDSAAVLLLELLN 1905

[Close]

BLAST of Chy7G144200 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.5e-04
Identity = 31/99 (31.31%), Postives = 44/99 (44.44%), Query Frame = 0


Query: 121  KDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180
            K+ A++    R G  K G   +   +  L +IC    W  P + C  E GP+HAK++ + 
Sbjct: 1776 KETATKKEDERDGEKKNGA--QMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAKRFVYS 1835

Query: 181  VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLN 220
            VR+    D     EC G      K A + AA   L  LN
Sbjct: 1836 VRVN-TSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLN 1871

[Close]

BLAST of Chy7G144200 vs. ExPASy TrEMBL
Match: A0A0A0KYI7 (DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.6e-126
Identity = 217/227 (95.59%), Postives = 222/227 (97.80%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEKS 60
           MRHY+DIVG +RKNRGIQMENNPSCPPPRLNINLKNLPPINNPRN+SGSHSSIPAKFEKS
Sbjct: 1   MRHYSDIVGPERKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKS 60

Query: 61  RYIRRVTQFMPSDHRPEIRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNC 120
           RYIRRV QFMPSDHRPEIRSNDYAGLPMKL+YSDSARALNTKT PVAPSADSFV+KKD C
Sbjct: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTC 120

Query: 121 KDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180
           KDLASRCSCCRH STKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK
Sbjct: 121 KDLASRCSCCRHDSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180

Query: 181 VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 228
           VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR
Sbjct: 181 VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of Chy7G144200 vs. ExPASy TrEMBL
Match: A0A5D3E0Z9 (Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001930 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.6e-116
Identity = 206/229 (89.96%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQME-NNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEK 60
           MRHYT+IVGA+RKNRGIQME NNPSC PPRLNINLKNLPPINNPRN++G+HSSIPAKFEK
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSC-PPRLNINLKNLPPINNPRNVTGTHSSIPAKFEK 60

Query: 61  SRYIRRVTQFMPSDHRPEIRSNDYAGLP-MKLSYSDSARALNTKTIPVAPSADSFVQKKD 120
           SRYIRRV QF+P DHRPEIRSN+Y GLP MKL+ SDSARA+NTKTIPVAPSAD FVQKKD
Sbjct: 61  SRYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKD 120

Query: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYR 180
           NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+KYR
Sbjct: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYR 180

Query: 181 FKVRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 228
           FKV IEMKGDCEAVVECYGNLQTRKK+AAEHAAEGALWYL +LGYRF+R
Sbjct: 181 FKVSIEMKGDCEAVVECYGNLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of Chy7G144200 vs. ExPASy TrEMBL
Match: A0A1S3BXK0 (uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.6e-116
Identity = 206/229 (89.96%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQME-NNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEK 60
           MRHYT+IVGA+RKNRGIQME NNPSC PPRLNINLKNLPPINNPRN++G+HSSIPAKFEK
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSC-PPRLNINLKNLPPINNPRNVTGTHSSIPAKFEK 60

Query: 61  SRYIRRVTQFMPSDHRPEIRSNDYAGLP-MKLSYSDSARALNTKTIPVAPSADSFVQKKD 120
           SRYIRRV QF+P DHRPEIRSN+Y GLP MKL+ SDSARA+NTKTIPVAPSAD FVQKKD
Sbjct: 61  SRYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKD 120

Query: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYR 180
           NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+KYR
Sbjct: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYR 180

Query: 181 FKVRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 228
           FKV IEMKGDCEAVVECYGNLQTRKK+AAEHAAEGALWYL +LGYRF+R
Sbjct: 181 FKVSIEMKGDCEAVVECYGNLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of Chy7G144200 vs. ExPASy TrEMBL
Match: A0A1S4DZP1 (uncharacterized protein LOC103494682 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 9.5e-87
Identity = 160/178 (89.89%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQME-NNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEK 60
           MRHYT+IVGA+RKNRGIQME NNPSC PPRLNINLKNLPPINNPRN++G+HSSIPAKFEK
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSC-PPRLNINLKNLPPINNPRNVTGTHSSIPAKFEK 60

Query: 61  SRYIRRVTQFMPSDHRPEIRSNDYAGLP-MKLSYSDSARALNTKTIPVAPSADSFVQKKD 120
           SRYIRRV QF+P DHRPEIRSN+Y GLP MKL+ SDSARA+NTKTIPVAPSAD FVQKKD
Sbjct: 61  SRYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKD 120

Query: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKK 177
           NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+K
Sbjct: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARK 177

[Close]

BLAST of Chy7G144200 vs. ExPASy TrEMBL
Match: A0A6J1GW48 (ribonuclease 3-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458030 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.3e-75
Identity = 144/221 (65.16%), Postives = 169/221 (76.47%), Query Frame = 0


Query: 3   HYTDIVGADRKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEKSRY 62
           HY +IVG +RKNR  QMEN PSC P  +N+NLKNLPPI+NP   + +HSSIPAKFEKSRY
Sbjct: 6   HYREIVGEERKNRENQMENTPSC-PRSVNVNLKNLPPIHNPCVGNANHSSIPAKFEKSRY 65

Query: 63  IRRVTQFMPSDHRPEIRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNCKD 122
           I RV QFMP +H P++R N+Y G+PM  +  DSAR+ N +T P         ++K+N  D
Sbjct: 66  IGRVPQFMPPNHSPDVRPNEYDGVPMNPACFDSARSPNARTTP--------KERKENFGD 125

Query: 123 LASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVR 182
            ASR S C H STKEGT  K++AKSLL+EICTANHW+PPLFECCEEEGPSHAKKY FKV 
Sbjct: 126 SASRSSGCSHDSTKEGTSVKKSAKSLLYEICTANHWKPPLFECCEEEGPSHAKKYTFKVI 185

Query: 183 IEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGY 224
           +EMKGDC   +ECYG  QTRKKVAAE AAEGA+WYL HLGY
Sbjct: 186 VEMKGDCGEDLECYGKPQTRKKVAAEDAAEGAVWYLKHLGY 217

[Close]

BLAST of Chy7G144200 vs. NCBI nr
Match: XP_004152191.1 (uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical protein Csa_014441 [Cucumis sativus])

HSP 1 Score: 461 bits (1185), Expect = 1.23e-163
Identity = 217/227 (95.59%), Postives = 222/227 (97.80%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEKS 60
           MRHY+DIVG +RKNRGIQMENNPSCPPPRLNINLKNLPPINNPRN+SGSHSSIPAKFEKS
Sbjct: 1   MRHYSDIVGPERKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKS 60

Query: 61  RYIRRVTQFMPSDHRPEIRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNC 120
           RYIRRV QFMPSDHRPEIRSNDYAGLPMKL+YSDSARALNTKT PVAPSADSFV+KKD C
Sbjct: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTC 120

Query: 121 KDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180
           KDLASRCSCCRH STKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK
Sbjct: 121 KDLASRCSCCRHDSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180

Query: 181 VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227
           VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR
Sbjct: 181 VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of Chy7G144200 vs. NCBI nr
Match: XP_008454206.1 (PREDICTED: uncharacterized protein LOC103494682 isoform X1 [Cucumis melo] >KAA0044459.1 ribonuclease 3-like protein 1 [Cucumis melo var. makuwa] >TYK29586.1 ribonuclease 3-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 427 bits (1097), Expect = 3.33e-150
Identity = 206/229 (89.96%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQME-NNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEK 60
           MRHYT+IVGA+RKNRGIQME NNPSCPP RLNINLKNLPPINNPRN++G+HSSIPAKFEK
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSCPP-RLNINLKNLPPINNPRNVTGTHSSIPAKFEK 60

Query: 61  SRYIRRVTQFMPSDHRPEIRSNDYAGLP-MKLSYSDSARALNTKTIPVAPSADSFVQKKD 120
           SRYIRRV QF+P DHRPEIRSN+Y GLP MKL+ SDSARA+NTKTIPVAPSAD FVQKKD
Sbjct: 61  SRYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKD 120

Query: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYR 180
           NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+KYR
Sbjct: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYR 180

Query: 181 FKVRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227
           FKV IEMKGDCEAVVECYGNLQTRKK+AAEHAAEGALWYL +LGYRF+R
Sbjct: 181 FKVSIEMKGDCEAVVECYGNLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of Chy7G144200 vs. NCBI nr
Match: XP_038878769.1 (ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 346 bits (888), Expect = 1.76e-118
Identity = 175/223 (78.48%), Postives = 191/223 (85.65%), Query Frame = 0


Query: 5   TDIVGADRKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEKSRYIR 64
           T+IV A+RKNRG QMEN  SCP  RLN NLKNLPPINN    +G+HSSI AKFEKSRYIR
Sbjct: 4   TEIVEAERKNRGNQMENTHSCPR-RLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIR 63

Query: 65  RVTQFMPSDHRPEIRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNCKDLA 124
           RV QF+P +HRP    N+YAGLPMK + S SARALN+KTIP APS DSFVQKKDN +++A
Sbjct: 64  RVPQFIPQNHRP----NEYAGLPMKPACSGSARALNSKTIPEAPS-DSFVQKKDNSQEIA 123

Query: 125 SRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIE 184
           SR SCC HGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKV +E
Sbjct: 124 SRSSCCGHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVE 183

Query: 185 MKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227
           MKG  E V+ECYGNLQTRKKVAAEHAAEGA+WYL +LGY FKR
Sbjct: 184 MKGASETVLECYGNLQTRKKVAAEHAAEGAIWYLKNLGYCFKR 220

[Close]

BLAST of Chy7G144200 vs. NCBI nr
Match: XP_016901448.1 (PREDICTED: uncharacterized protein LOC103494682 isoform X2 [Cucumis melo])

HSP 1 Score: 330 bits (845), Expect = 2.68e-112
Identity = 160/178 (89.89%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MRHYTDIVGADRKNRGIQME-NNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEK 60
           MRHYT+IVGA+RKNRGIQME NNPSCPP RLNINLKNLPPINNPRN++G+HSSIPAKFEK
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSCPP-RLNINLKNLPPINNPRNVTGTHSSIPAKFEK 60

Query: 61  SRYIRRVTQFMPSDHRPEIRSNDYAGLP-MKLSYSDSARALNTKTIPVAPSADSFVQKKD 120
           SRYIRRV QF+P DHRPEIRSN+Y GLP MKL+ SDSARA+NTKTIPVAPSAD FVQKKD
Sbjct: 61  SRYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKD 120

Query: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKK 176
           NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+K
Sbjct: 121 NCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARK 177

[Close]

BLAST of Chy7G144200 vs. NCBI nr
Match: XP_038878786.1 (ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 330 bits (846), Expect = 3.67e-112
Identity = 167/212 (78.77%), Postives = 182/212 (85.85%), Query Frame = 0


Query: 5   TDIVGADRKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNISGSHSSIPAKFEKSRYIR 64
           T+IV A+RKNRG QMEN  SCP  RLN NLKNLPPINN    +G+HSSI AKFEKSRYIR
Sbjct: 4   TEIVEAERKNRGNQMENTHSCPR-RLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIR 63

Query: 65  RVTQFMPSDHRPEIRSNDYAGLPMKLSYSDSARALNTKTIPVAPSADSFVQKKDNCKDLA 124
           RV QF+P +HRP    N+YAGLPMK + S SARALN+KTIP APS DSFVQKKDN +++A
Sbjct: 64  RVPQFIPQNHRP----NEYAGLPMKPACSGSARALNSKTIPEAPS-DSFVQKKDNSQEIA 123

Query: 125 SRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIE 184
           SR SCC HGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKV +E
Sbjct: 124 SRSSCCGHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVE 183

Query: 185 MKGDCEAVVECYGNLQTRKKVAAEHAAEGALW 216
           MKG  E V+ECYGNLQTRKKVAAEHAAEGA+W
Sbjct: 184 MKGASETVLECYGNLQTRKKVAAEHAAEGAIW 209

[Close]

BLAST of Chy7G144200 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 106.7 bits (265), Expect = 2.5e-23
Identity = 46/88 (52.27%), Postives = 58/88 (65.91%), Query Frame = 0


Query: 136  KEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVEC 195
            K G P  + AKSLL E C AN W+PP FECCEEEGP H K + +KV +E++      +EC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 196  YGNLQTRKKVAAEHAAEGALWYLNHLGY 224
            YG  +  KK AAEHAA+ A+W L H G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Chy7G144200 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 106.7 bits (265), Expect = 2.5e-23
Identity = 46/88 (52.27%), Postives = 58/88 (65.91%), Query Frame = 0


Query: 136  KEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVEC 195
            K G P  + AKSLL E C AN W+PP FECCEEEGP H K + +KV +E++      +EC
Sbjct: 1599 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1658

Query: 196  YGNLQTRKKVAAEHAAEGALWYLNHLGY 224
            YG  +  KK AAEHAA+ A+W L H G+
Sbjct: 1659 YGEARATKKGAAEHAAQAAIWCLKHSGF 1686

[Close]

BLAST of Chy7G144200 vs. TAIR 10
Match: AT1G80650.1 (RNAse THREE-like protein 1 )

HSP 1 Score: 98.6 bits (244), Expect = 6.9e-21
Identity = 39/86 (45.35%), Postives = 61/86 (70.93%), Query Frame = 0


Query: 137 EGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDC-EAVVEC 196
           E    + +AKS+L E+C +  W+PP++ECC  +GP H + + +KV +E++    + V+EC
Sbjct: 107 ESKTRRGSAKSVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLEC 166

Query: 197 YGNLQTRKKVAAEHAAEGALWYLNHL 222
           +G+ + +KK AAEHAAEGALWYL H+
Sbjct: 167 FGDPRRKKKAAAEHAAEGALWYLEHV 192

[Close]

BLAST of Chy7G144200 vs. TAIR 10
Match: AT4G00420.2 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-19
Identity = 44/90 (48.89%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 135 TKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCE-AVV 194
           T E   ++ +AKS L+ +C+  HW+ PL+E    EGP H K +  KV +EMK D    V+
Sbjct: 75  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 134

Query: 195 ECYGNLQTRKKVAAEHAAEGALWYLNHLGY 224
           EC+GN Q +KK+AAE AAE ALWYL ++GY
Sbjct: 135 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 163

[Close]

BLAST of Chy7G144200 vs. TAIR 10
Match: AT4G00420.3 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-19
Identity = 44/90 (48.89%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 135 TKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCE-AVV 194
           T E   ++ +AKS L+ +C+  HW+ PL+E    EGP H K +  KV +EMK D    V+
Sbjct: 53  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 112

Query: 195 ECYGNLQTRKKVAAEHAAEGALWYLNHLGY 224
           EC+GN Q +KK+AAE AAE ALWYL ++GY
Sbjct: 113 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 141

The following BLAST results are available for this feature:

BLAST of Chy7G144200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P84634	3.6e-22	52.27	Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2	[more]
A7LFZ6	6.7e-21	50.62	Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
Q9M8N2	9.7e-20	45.35	Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1	[more]
Q9SP32	3.0e-05	27.46	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]
Q8LMR2	1.5e-04	31.31	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]

BLAST of Chy7G144200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYI7	1.6e-126	95.59	DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 S...	[more]
A0A5D3E0Z9	2.6e-116	89.96	Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BXK0	2.6e-116	89.96	uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S4DZP1	9.5e-87	89.89	uncharacterized protein LOC103494682 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1GW48	1.3e-75	65.16	ribonuclease 3-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Chy7G144200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152191.1	1.23e-163	95.59	uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical ...	[more]
XP_008454206.1	3.33e-150	89.96	PREDICTED: uncharacterized protein LOC103494682 isoform X1 [Cucumis melo] >KAA00...	[more]
XP_038878769.1	1.76e-118	78.48	ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 rib...	[more]
XP_016901448.1	2.68e-112	89.89	PREDICTED: uncharacterized protein LOC103494682 isoform X2 [Cucumis melo]	[more]
XP_038878786.1	3.67e-112	78.77	ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida]	[more]

BLAST of Chy7G144200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20320.1	2.5e-23	52.27	dicer-like 4	[more]
AT5G20320.2	2.5e-23	52.27	dicer-like 4	[more]
AT1G80650.1	6.9e-21	45.35	RNAse THREE-like protein 1	[more]
AT4G00420.2	3.8e-19	48.89	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]
AT4G00420.3	3.8e-19	48.89	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 145..219 e-value: 2.1E-8 score: 43.9
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 144..220 score: 9.513474
None	No IPR available	GENE3D	3.30.160.20		coord: 151..220 e-value: 4.6E-21 score: 77.1
None	No IPR available	PFAM	PF14709	DND1_DSRM	coord: 145..218 e-value: 1.6E-14 score: 54.0
None	No IPR available	PANTHER	PTHR14950:SF67	DOUBLE-STRANDED RNA-BINDING DOMAIN (DSRBD)-CONTAINING PROTEIN	coord: 11..227
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 11..227
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 145..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy7G144200.1	Chy7G144200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding